CN214584918U

CN214584918U - Bioluminescence bacterium culture and detection device

Info

Publication number: CN214584918U
Application number: CN202120406586.7U
Authority: CN
Inventors: 唐怀武; 周磊; 胡兴斌; 祝佳杰; 蒋自然; 周荣
Original assignee: Hangzhou Chunlai Technology Co Ltd
Current assignee: Hangzhou Chunlai Technology Co Ltd
Priority date: 2021-02-24
Filing date: 2021-02-24
Publication date: 2021-11-02
Anticipated expiration: 2031-02-24

Abstract

The utility model belongs to the technical field of biological toxicity detection, in particular to a bioluminescent bacterium culture and detection device, which comprises a box body, a culture tank, a bacterium storage box and a detector, wherein the culture tank is provided with a plurality of bacteria, the culture tank and the bacterium storage box are arranged in the box body, the detector is fixedly connected with the culture tank, a needle tube is arranged in the box body, and the needle tube is used for transferring a bacterium solution from the bacterium storage box to the culture tank; culturing the bacterial liquid through a culture tank, and detecting through a detector; the utility model discloses can realize the cultivation and the detection integration to the fungus liquid, improve simultaneously and detect the precision, it is more convenient to use.

Description

Bioluminescence bacterium culture and detection device

Technical Field

The utility model belongs to the technical field of biological toxicity detects, concretely relates to detection device is cultivateed to bioluminescence fungus.

Background

The overall technology of the biotoxicity online analysis equipment is mature day by day, and a luminous bacteria method is adopted for more quickly reflecting the comprehensive toxicity and the change of the water body. The luminous bacteria method is to mix and culture luminous bacteria with water and pure water, and measure the change difference before and after the luminous bacteria culture to determine the toxicity level of the water.

The luminescent bacteria method needs a specific culture device, and generally adopts a TEC refrigeration mode, so that the water body and the bacteria for reaction are cultured at a specific temperature, and errors are reduced. The refrigeration mode adopts and directly pastes on culture apparatus for culture apparatus can appear heating inhomogeneous phenomenon. Because general detection equipment cannot meet the requirement that culture and detection are carried out simultaneously, and inconvenience is brought in the using process, the improvement is needed to overcome the defects in practical application.

SUMMERY OF THE UTILITY MODEL

Based on the above-mentioned shortcomings and deficiencies of the prior art, it is an object of the present invention to at least solve one or more of the above-mentioned problems of the prior art, in other words, to provide a bioluminescent bacteria culture detection device that meets one or more of the above-mentioned needs.

In order to achieve the purpose of the utility model, the utility model adopts the following technical scheme:

a bioluminescent bacterium culture device comprises a box body, a culture tank, a bacterium storage box and a detector, wherein the culture tank is provided with a plurality of culture tanks, the culture tanks and the bacterium storage box are arranged in the box body, the detector is fixedly connected with the culture tank, and a needle tube is arranged in the box body and used for transferring bacterium liquid from the bacterium storage box to the culture tank; the bacteria liquid is cultured through the culture tank, and detection is realized through the detector.

Preferably, the two culture tanks are respectively provided with a first base, the first base is fixedly connected with a first pressing sheet, and a first gasket is arranged on the first pressing sheet.

Preferably, the first gasket is provided with a first puncture hole.

As a preferred scheme, a second base is installed on the bacterium storage box and fixedly connected with a second pressing sheet, and a second gasket is installed on the second pressing sheet.

Preferably, the second gasket is provided with a second puncture hole.

Preferably, the needle tube has a sampling needle that engages the first puncture and the second puncture.

Preferably, the outer wall of the box body is provided with a control device.

Preferably, the box body is provided with a refrigerator, a heater and a radiator.

Preferably, the detector, the refrigerator, the heater and the control device are electrically connected.

As a preferred scheme, a transmission shaft is arranged in the box body and comprises an X transmission shaft, a Y transmission shaft and a Z transmission shaft, and the Z transmission shaft is connected with the needle tube so that the needle tube can move horizontally along the X axis, the Y axis and the Z axis of the box body.

Compared with the prior art, the utility model, beneficial effect is:

the utility model discloses a detection device is cultivateed to bioluminescence fungus can realize going on with the detection integration to the cultivation of fungus liquid, has improved the detection precision simultaneously, and it is more convenient to use.

The utility model discloses a detection device is cultivateed to bioluminescence fungus realizes taking a sample and advances the appearance in-process at the needle puncture technique, can prevent to receive external parasitic light interference, avoids external light to get into simultaneously, and it is higher to detect rate of accuracy and precision.

The utility model discloses a detection device is cultivateed to bioluminescence fungus through X transmission shaft, Y transmission shaft and the Z transmission shaft in the box, mutually supports through three mutually perpendicular's transmission shaft, can guarantee that the shift position of needle tubing adjusts at any time in the box to the realization shifts the fungus liquid that stores up the fungus case to the incubator in, the volume of effectual reduction equipment improves the portability.

Drawings

Fig. 1 is a schematic view of an overall structure of a box according to a first embodiment of the present invention;

FIG. 2 is a cross-sectional view of an incubator according to a first embodiment of the present invention;

FIG. 3 is a top view of the incubator according to the first embodiment of the present invention;

FIG. 4 is a front view of an incubator according to a first embodiment of the present invention;

FIG. 5 is a rear view of the incubator according to the first embodiment of the present invention;

FIG. 6 is a front view of the bacteria storage box according to the first embodiment of the present invention;

FIG. 7 is a side view of the bacteria storage box according to the first embodiment of the present invention;

FIG. 8 is a flowchart illustrating the cultivation and detection of bioluminescent bacteria according to the first embodiment of the present invention;

in the figure: the device comprises a box body 1, a detector support 11, a heat conducting block 12, a fin 13, a fan 14, a control device 15, a culture tank 2, a first base 21, a first pressing sheet 22, a first arc surface 221, a first gasket 23, a bacteria storage tank 3, a second base 31, a second pressing sheet 32, a second arc surface 321, a second gasket 33, a detector 4, a refrigerator 5, a heater 6, a radiator 7, a water sample 8, a sample introduction system 9, a reaction chamber 91, a peristaltic pump 92, a sample cell 93, a reference cell 10, a bacteria storage cell 10, a sample introduction system 101, a bacteria liquid cell 102, a cleaning cell 103, a waste liquid cell 1101, an X transmission shaft 1102Y transmission shaft and a 1103Z transmission shaft.

Detailed Description

In order to more clearly illustrate the embodiments of the present invention, the following description will explain embodiments of the present invention with reference to the accompanying drawings. It is obvious that the drawings in the following description are only examples of the invention, and that for a person skilled in the art, other drawings and embodiments can be obtained from these drawings without inventive effort.

The first embodiment is as follows:

as shown in fig. 1 to 7, the present embodiment provides a bioluminescent bacteria culture detection apparatus, which includes a case 1, a culture tank 2, a bacteria storage tank 3 and a detector 4, wherein the culture tank 2 has a plurality of culture tanks 2, the culture tanks 2 and the bacteria storage tank 3 are installed in the case 1, the detector 4 is fixedly connected to the culture tank 2, and the culture tank 2 is used for culturing bacteria liquid; a needle tube is arranged in the box body 1, bacterium liquid is transferred from the bacterium storage box to the culture tank 2 through the needle tube, and detection is realized through a detector 4.

Specifically, installation detector 4 in box 1, detector 4 installs on detector support 11, and detector 4 and culture tank 2 fixed connection can realize the fungus liquid real-time detection to culture tank 2 through detector 4.

Cultivate jar 2 and have two, two cultivate jar 2 and install first base 21 respectively, the first preforming 22 of first base 21 fixed connection, first arc surface 221 has on the first preforming 22, install first gasket 23 on the first arc surface 221, first puncture hole has been seted up on the first gasket 23, first puncture hole cooperatees with the sample needle of needle tubing, in order to realize shifting the fungus liquid of needle tubing to cultivate jar 2, two cultivate jars in this embodiment are as realizing group and contrast respectively, thereby can realize the contrast experiment. The number of culture tanks can be set according to actual requirements, and is not limited to the number mentioned above so as to meet the actual application.

Store up and install second base 31 on fungus case 3, second base 31 fixed connection second preforming 32, second preforming 32 has second arc surface 321, second gasket 33 is installed to second arc surface 321, the second puncture hole has been seted up on second gasket 33, the second puncture hole cooperatees with the sampling needle of needle tubing, in order to realize shifting the fungus liquid that stores up in fungus case 3 to the needle tubing, the sampling needle is at sample and advance kind in-process, can prevent that the fungus liquid from revealing.

This embodiment can realize transferring the fungus liquid in succession through the cooperation between sample needle and first puncture and the second puncture to detect the fungus liquid in the culture tank 2 in real time through detector 4, thereby realize cultivateing and detect the integration.

Through the sample introduction of needle puncture technique to the sample of storing up the fungus case and cultivateing the jar, prevent that the fungus liquid in the cultivation jar from receiving external miscellaneous light interference in the testing process, can avoid external light to get into simultaneously to avoid influencing the cultivation quality to the fungus liquid.

Refrigerator 5 is installed to box 1, heater 6 and radiator 7, the water and the bacterium that can guarantee the reaction are cultivateed under specific temperature, be favorable to the bacterial to cultivate under suitable temperature, install fan 14 between heater 6 and the detector 4, fan 14 can accelerate the heat flow, make cultivation jar 2 be heated evenly, refrigerator 5 fixed connection heat conduction piece 12, heat conduction piece 12 can play the function of heat conduction, still install fin 13 in the box 1, in order to reach the purpose that improves heat exchange efficiency.

Install controlling means 12 on the outer wall of box 1, detector 4, refrigerator 5 and heater 6 respectively with controlling means 12 electric connection, can realize being heated evenly with the assurance culture tank 2 to the automated inspection and the automatically regulated temperature of culture tank 2 through controlling means 12.

The transmission shaft is arranged in the box body in the embodiment, the transmission shaft comprises an X transmission shaft 1101, a Y transmission shaft 1102 and a Z transmission shaft 1103, the Z transmission shaft 1103 is fixedly connected with the needle tube, the X transmission shaft 1101, the Y transmission shaft 1102 and the Z transmission shaft 1103 are perpendicular to each other and are respectively arranged on the inner wall of the box body 1, so that the needle tube can move horizontally along the X axis, the Y axis and the Z axis of the box body, the three transmission shafts which are perpendicular to each other are matched with each other, the moving position of the needle tube can be guaranteed to be adjusted at any time in the box body, and the use convenience is improved.

As shown in fig. 8, the water sample, sampling system 8 includes quality control sample, water sample, brine, waste water, pure water and standard sample, reaction chamber 9 includes sample cell 92 and reference cell 93, the bioluminescence bacteria culture detection device of this embodiment is in the use, at first cooperate through syringe pump and peristaltic pump 91, use the water to wash the culture tank, the culture tank is divided into sample tank and comparison tank, then through the X transmission shaft, Y transmission shaft and Z transmission shaft move the needle tubing to the second gasket department of storing up the fungus case, so that the sample needle and the second puncture hole cooperation of needle tubing, store in the bacterial, the fungus liquid pond 101 of sampling system 10 takes out the right amount of fungus liquid, through the sample needle and the first puncture hole cooperation of needle tubing, in order to shift the fungus liquid to the culture tank, thereby realize the fungus liquid and shift.

Culturing strains in culture tanks to a target state, metering a proper amount of pure water by using an injection pump, placing the strains in one of the culture tanks for culture, detecting the culture condition in real time by using a detector, and discharging waste liquid into a waste liquid pool 103 through a peristaltic pump after the culture is finished; and finally, cleaning the culture tank by using water through the mutual matching of the injection pump and the peristaltic pump, and discharging the cleaned liquid into the cleaning pool 102.

The foregoing has been a detailed description of the preferred embodiments and principles of the present invention, and it will be apparent to those skilled in the art that variations may be made in the specific embodiments based on the concepts of the present invention, and such variations are considered as within the scope of the present invention.

Claims

1. A bioluminescent bacterium culture detection device is characterized by comprising a box body, a culture tank, a bacterium storage box and a detector, wherein the culture tank is provided with a plurality of culture tanks, the culture tanks and the bacterium storage box are arranged in the box body, the detector is fixedly connected with the culture tank, and needle tubes are arranged in the box body and used for transferring bacterium liquid from the bacterium storage box to the culture tank; the bacteria liquid is cultured through the culture tank, and detection is realized through the detector.

2. The apparatus as claimed in claim 1, wherein the two culture tanks have first bases, the first bases are fixedly connected to the first pressing plate, and the first pressing plate has a first gasket.

3. The apparatus according to claim 2, wherein the first spacer has a first piercing hole.

4. The apparatus according to claim 3, wherein a second base is mounted on the bacteria storage box, the second base is fixedly connected to a second pressing plate, and a second gasket is mounted on the second pressing plate.

5. The apparatus according to claim 4, wherein the second gasket has a second piercing hole.

6. The apparatus according to claim 5, wherein the needle tube has a sampling needle, and the sampling needle is engaged with the first piercing hole and the second piercing hole.

7. The apparatus according to claim 1, wherein the control device is mounted on an outer wall of the housing.

8. The apparatus of claim 6, wherein the housing is provided with a refrigerator, a heater and a heat sink.

9. The apparatus according to claim 7, wherein the detector, the refrigerator and the heater are electrically connected to the control device.

10. The apparatus according to claim 1, wherein the box body is provided with a transmission shaft, the transmission shaft comprises an X-transmission shaft, a Y-transmission shaft and a Z-transmission shaft, and the Z-transmission shaft is connected with the needle tube so as to enable the needle tube to move horizontally along the X-axis, the Y-axis and the Z-axis of the box body.