CN217127433U

CN217127433U - Soil fluctuation anaerobic culture device

Info

Publication number: CN217127433U
Application number: CN202121129396.1U
Authority: CN
Inventors: 叶成龙; 杨家伟; 白彤硕; 吴彬
Original assignee: Nanjing Agricultural University
Current assignee: Nanjing Agricultural University
Priority date: 2021-05-25
Filing date: 2021-05-25
Publication date: 2022-08-05
Anticipated expiration: 2031-05-25

Abstract

The utility model discloses a undulant anaerobism culture apparatus of soil, include gas holder, gas distribution room and the blake bottle that connects gradually through the rubber tube, utilize the device to fluctuate the respiratory survey of microorganism under the anaerobic condition, can effectively avoid the interference of outside air, create stable anaerobism or aerobic condition and carry out required experiment, reach the purpose of the undulant anaerobic condition of accurate measurement soil microorganism respiration rate down.

Description

Soil fluctuation anaerobic culture device

Technical Field

The utility model relates to a undulant anaerobism culture apparatus of soil especially relates to a culture apparatus that is used for undulant anaerobism condition under soil microorganism respiration survey, belongs to culture apparatus technical field.

Background

Many surface soils on earth will undergo frequent alternation of wetting and drying due to changes in global water circulation caused by climate change. The alternation of soil moisture and dryness can not only affect the moisture change of soil, but also cause the fluctuation of soil oxygen, and the content of soil oxygen is an important factor affecting the respiration of soil microorganisms. Under the general condition, under the condition that the oxygen in the soil is sufficient, the microbial activity of the soil is higher, and the respiration rate is higher; in the absence of oxygen, the activity of soil microorganisms is inhibited and microorganisms undergo anaerobic respiration. In the case of the soil being subjected to fluctuating anaerobes, the change in the respiration rate of the soil microorganisms is not easily predictable, since other physicochemical factors in the soil will also change in this case.

By establishing a reliable culture system for simulating a fluctuation anaerobic state, the respiration rate of soil microorganisms under the fluctuation anaerobic condition is accurately measured and an internal mechanism is explored, however, the existing culture system is complicated to operate and is easily interfered by external air, and the problem of ventilation of a large batch of samples cannot be solved. Therefore, it is highly desirable to establish a culture device capable of measuring soil microorganism respiration under fluctuating anaerobic conditions, which is easy to operate, has good air tightness and high efficiency, and solves the above problems.

Disclosure of Invention

The utility model aims to overcome the not enough among the prior art, provide a undulant anaerobism culture apparatus of soil, the convenience is surveyed soil microorganism breathing under undulant anaerobic condition.

In order to achieve the above purpose, the utility model adopts the technical proposal that:

the utility model provides a soil fluctuation anaerobic culture device, which comprises an air storage tank, an air distribution chamber and a plurality of culture bottles which are connected in sequence through rubber pipes;

at least 2 vent needles are arranged on the culture bottle, and at least one vent needle is connected with a three-way valve;

the gas distribution chamber comprises a gas distribution chamber gas inlet hole and a plurality of gas distribution chamber gas outlet holes, and the gas distribution chamber gas outlet holes are connected with a three-way valve connected with the ventilating needle head through rubber pipes.

Furthermore, the number of the air storage tanks is at least 2, and the air storage tanks are used for respectively storing high-purity nitrogen and air without carbon dioxide.

Further, the device still includes the temperature control board, the temperature control board sets up in the blake bottle bottom for the temperature of adjustment cultivation.

Further, the diameter of the needle hole of the vent needle is not less than 1.2 mm.

Further, the device also comprises a humidifying bottle which is connected between the gas storage tank and the gas distribution chamber through a rubber pipe.

Furthermore, a rubber pipe connected with the air storage tank is introduced into the liquid in the humidifying bottle.

Further, the humidifying bottle and the culture bottle are provided with rubber stoppers for sealing.

Further, a gas flow rate control valve is arranged on a rubber plug of the humidifying bottle and/or the culture bottle.

Compared with the prior art, the utility model discloses the beneficial effect who reaches:

the utility model provides a soil fluctuation anaerobic culture device, which has good integral air tightness, guarantees the anaerobic environment in the culture bottle and reduces the error; different gas storage tanks are adopted to switch anaerobic and aerobic states at any time, so that the requirement of experimental design is met; ensuring the flow rate of the gas at each outlet to be consistent through the gas distribution chamber, and introducing the gas with the same flow rate and components into the batch culture bottles; the temperature control plate is arranged to ensure that the culture bottle is in a constant temperature state, and the influence of temperature fluctuation on soil microorganisms is avoided.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a soil fluctuation anaerobic culture device according to an embodiment of the present invention;

in the figure: 1. a first gas storage tank; 2. a second gas tank; 3. a second rubber tube; 4. a first rubber tube; 5. A first rubber plug; 6. a humidifying bottle; 7. a third rubber tube; 8. a gas distribution chamber; 9. a second rubber plug; 10. A culture bottle; 11. a first syringe needle; 12. a second syringe needle; 13. a first three-way valve; 14. A second three-way valve; 15. a fourth rubber tube; 16. a temperature control plate; 17. gas distribution chamber inlet ports; 18. and air outlet holes of the gas distribution chamber.

Detailed Description

The present invention will be further described with reference to the accompanying drawings, and the following embodiments are only used to illustrate the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate a number of the indicated technical features. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

As shown in fig. 1, for the utility model provides a soil fluctuation anaerobism culture apparatus's overall structure sketch map, a culture apparatus for fluctuating anaerobism condition down soil microorganism respiration survey, including first gas holder 1 and second gas holder 2, a first rubber buffer 5 for sealing first gas holder 1 and second gas holder 2, humidification bottle 6 that links to each other with the gas holder through the rubber tube, be linked together with humidification bottle 6 through the rubber tube, the gas distribution room 8 that has a gas distribution room inlet port 17 and a plurality of gas distribution room venthole 18 links to each other, gas distribution room 8's gas distribution room venthole 18 loops through the rubber tube, three-way valve and syringe needle link to each other with the blake bottle that is used for cultivateing soil, the blake bottle is placed on temperature control plate 16, can keep the constancy of temperature during the cultivation.

Wherein be equipped with the rubber buffer that is used for sealed on the blake bottle, be equipped with first syringe needle 11 and second syringe needle 12 on this rubber buffer, first syringe needle 11 is connected with arbitrary gas distribution room venthole 18 through first three-way valve 13 and fourth rubber tube 15, and second syringe needle 12 is connected with second three-way valve 14, can use the syringe to extract the gas in the blake bottle through second three-way valve 14, carries out assay of ingredient concentration such as CO2 in the blake bottle.

First gas holder 1 is linked together through first rubber tube 4 and humidification bottle 6, and second gas holder 2 is linked together through second rubber tube 3 and humidification bottle 6, and first gas holder 1 and second gas holder 2 are used for conveying gas in to humidification bottle 6.

A certain amount of sterile water is filled in the humidifying bottle 6, the second rubber tube 3 and the first rubber tube 4 are inserted into the water, and the third rubber tube 7 is not inserted into the water, so that the effect of gas humidification is achieved. The gas is connected with a gas distribution chamber air inlet 17 through a third rubber tube 7, moist gas is introduced into the gas distribution chamber 8, and then the moist gas is introduced into the culture bottle through a gas distribution chamber air outlet 18 and a fourth rubber tube 15.

The culture flask is sealed with second rubber buffer 9, has inserted first syringe needle 11 and the second syringe needle 12 that is used for giving vent to anger on the second rubber buffer 9, and the preferred 1.2mm of needle diameter of first syringe needle 11 and second syringe needle 12.

The determination of the respiration of the microorganism under the fluctuation anaerobic condition is carried out by the soil fluctuation anaerobic culture device, the first air storage tank 1 and the second air storage tank 2 can be filled with high-purity nitrogen and carbon dioxide-free air, and the specific operation process is as follows:

example 1:

if the soil is subjected to anaerobic culture, firstly introducing high-purity nitrogen, completely replacing the gas in the culture bottle, closing the first three-way valve 13 and the second three-way valve 14, and then standing for culture;

example 2:

if the soil is to be cultured in the presence of oxygen, carbon dioxide-free air is introduced to completely replace the gas in the culture flask, and the three-

way valves

13 and 14 are closed to perform stationary culture. If the soil microorganism respiration is to be measured, after culturing for a certain period of time, an injector is inserted into the second three-way valve 14, the valve of the three-way valve 14 is opened, the gas in the culture flask is pumped out, and the gas chromatography is used for CO ₂ And (4) measuring the concentration.

The utility model provides a undulant anaerobism culture apparatus of soil is arranged in the air to be equipped with in the blake bottle of the soil that awaits measuring evenly ventilates among the displacement device and builds anaerobic environment to different gas is let in according to the experiment demand of difference.

This device can ventilate simultaneously to large batch soil blake bottle, can keep the constancy of temperature with temperature control board 16 simultaneously, guarantees to let in the gaseous composition in the blake bottle, the velocity of flow and the temperature of blake bottle the same to get rid of the interference of other factors when ventilating.

Furthermore, a rubber plug of the humidifying bottle and/or the culture bottle is provided with a gas flow rate control valve, so that the gas flow rate can be set according to different test requirements, and the microbial respiration results in different culture environments can be measured.

The utility model provides a pair of undulant anaerobism culture apparatus of soil, whole gas tightness is good, and isolated outside air has avoided the outside air to get into and destroys the anaerobic environment and miscellaneous fungus entering interference soil microorganism, guarantees the anaerobic environment in the blake bottle, prevents the production error. The device adopts two different gas holders, is equipped with high-purity nitrogen gas and no carbon dioxide air respectively, can switch anaerobism and aerobic state at any time, can satisfy the needs of different experimental design. The gas distribution room that adopts can guarantee that the velocity of flow of each exit gas is unanimous, realizes letting in the gas of the same velocity of flow and composition simultaneously to batch culture bottle, can guarantee through the temperature control board that the culture bottle is in the constant temperature state, avoids the influence of temperature fluctuation to soil microorganism. The three-way valve, the syringe needle, the culture bottle, the rubber tube and other raw materials used by the device are convenient to obtain, simple to assemble, economical and practical, and meet the requirements of most laboratories.

In conclusion, the utility model provides a undulant anaerobic culture device of soil can guarantee to let in the stable standard gas of homogeneous, effectively avoids the interference of outside air, creates stable anaerobism or aerobic condition and carries out required test, reaches the purpose of the undulant anaerobic condition of accurate measurement soil microorganism respiratory rate down.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be considered as the protection scope of the present invention.

Claims

1. A soil fluctuation anaerobic culture device is characterized by comprising an air storage tank, an air distribution chamber and a plurality of culture bottles which are sequentially connected through rubber pipes;

at least 2 vent needles are arranged on the culture bottle, wherein at least one vent needle is connected with a three-way valve;

2. The anaerobic cultivation device for soil fluctuation according to claim 1, wherein the number of the gas storage tanks is at least 2, and high purity nitrogen gas and carbon dioxide-free air are stored respectively.

3. The soil wave anaerobic culture device of claim 1, further comprising a temperature control plate disposed at the bottom of the culture flask for adjusting the temperature of the culture.

4. The soil fluctuation anaerobic cultivation device as claimed in claim 1, wherein the diameter of the pinhole of the aeration needle is not less than 1.2 mm.

5. The anaerobic soil wave culture device as claimed in claim 1, further comprising a humidifying bottle connected between the gas storage tank and the gas distribution chamber through a rubber tube.

6. The anaerobic culture device for soil fluctuation according to claim 5, wherein the rubber tube connected with the air storage tank is communicated with the interior of the liquid in the humidifying bottle.

7. A soil fluctuation anaerobic cultivation device as claimed in claim 5, wherein the humidifying bottle and the cultivation bottle are provided with rubber stoppers for sealing.

8. The soil wave anaerobic culture device of claim 7, wherein the rubber plugs of the humidifying bottles and/or the culture bottles are provided with gas flow rate control valves.