CN112996901A - 分离装置及使用该分离装置对分离对象物进行分离的方法 - Google Patents

分离装置及使用该分离装置对分离对象物进行分离的方法 Download PDF

Info

Publication number
CN112996901A
CN112996901A CN201980074086.3A CN201980074086A CN112996901A CN 112996901 A CN112996901 A CN 112996901A CN 201980074086 A CN201980074086 A CN 201980074086A CN 112996901 A CN112996901 A CN 112996901A
Authority
CN
China
Prior art keywords
separation
chamber
cells
suspension
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201980074086.3A
Other languages
English (en)
Chinese (zh)
Inventor
畑中大辅
木田克彦
金木达朗
林寿人
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nissan Chemical Corp
Original Assignee
Nissan Chemical Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nissan Chemical Corp filed Critical Nissan Chemical Corp
Publication of CN112996901A publication Critical patent/CN112996901A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16Particles; Beads; Granular material; Encapsulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22Settling tanks; Sedimentation by gravity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02Separating microorganisms from the culture medium; Concentration of biomass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201980074086.3A 2018-09-11 2019-09-11 分离装置及使用该分离装置对分离对象物进行分离的方法 Pending CN112996901A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018170092 2018-09-11
JP2018-170092 2018-09-11
PCT/JP2019/035651 WO2020054755A1 (ja) 2018-09-11 2019-09-11 分離デバイスおよびそれを用いて分離対象物を分離する方法

Publications (1)

Publication Number Publication Date
CN112996901A true CN112996901A (zh) 2021-06-18

Family

ID=69777887

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980074086.3A Pending CN112996901A (zh) 2018-09-11 2019-09-11 分离装置及使用该分离装置对分离对象物进行分离的方法

Country Status (6)

Country Link
US (2) US20220041971A1 (de)
EP (1) EP3848451A4 (de)
JP (1) JP7501365B2 (de)
CN (1) CN112996901A (de)
TW (1) TW202024314A (de)
WO (1) WO2020054755A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2019333933A1 (en) 2018-09-06 2021-05-13 Sand Separation Technologies Inc. Counterflow vortex breaker
WO2020232183A1 (en) * 2019-05-15 2020-11-19 Life Technologies Corporation Cell settler apparatus systems and methods for perfusion processes
US20230116841A1 (en) * 2020-04-03 2023-04-13 Shanghai Wolwo Stem Cell Technology Co., Ltd. Method for obtaining endometrial mesenchymal stem cells from human menstrual blood
CN114058505B (zh) * 2020-07-30 2024-10-01 环球生命科技咨询美国有限责任公司 新型灌流培养用高密度微载体截留装置和使用方法
JP1699777S (de) 2021-03-05 2021-11-15
KR20240004486A (ko) * 2021-04-30 2024-01-11 우시오덴키 가부시키가이샤 배양 용기 및 배양 용기의 사용 방법
JP2023124468A (ja) 2022-02-25 2023-09-06 藤森工業株式会社 細胞分離装置および細胞分離方法
WO2024206199A1 (en) * 2023-03-31 2024-10-03 Corning Incorporated Liquid handling device for shearing and filtration of biological matter

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102575219A (zh) * 2009-08-26 2012-07-11 艾克塞勒雷克斯公司 连续回收收获袋
US20130081995A1 (en) * 2011-09-29 2013-04-04 Hyclone Laboratories, Inc. Filter systems for separating microcarriers from cell culture solutions
WO2016002505A1 (ja) * 2014-06-30 2016-01-07 株式会社カネカ フィルター内の隆起部の外形を最適化したフィルター
JP2016010394A (ja) * 2014-06-30 2016-01-21 株式会社カネカ フィルター内の隆起部の比率を最適化したフィルター
US20160251628A1 (en) * 2013-10-30 2016-09-01 Exmoor Pharma Concepts Ltd Apparatus And Method For Filtration Of A Suspension
WO2018123619A1 (ja) * 2016-12-26 2018-07-05 東洋製罐グループホールディングス株式会社 フィルタ付き細胞用バッグ、及びその製造方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5232976B1 (de) 1976-01-16 1977-08-25
US8986944B2 (en) * 2001-10-11 2015-03-24 Aviva Biosciences Corporation Methods and compositions for separating rare cells from fluid samples
JP2005270891A (ja) 2004-03-26 2005-10-06 Tetsuo Kondo 多糖類の湿式粉砕方法
CN102337200A (zh) * 2010-07-20 2012-02-01 北京心意圆成生物科技有限公司 一种高效微载体细胞-培养基分离装置
ES2663225T3 (es) * 2013-09-30 2018-04-11 Westfälische Wilhelms-Universität Münster Método de enriquecimiento o aislamiento de una población de células diana
US10487308B2 (en) 2014-01-23 2019-11-26 Nissan Chemical Corporation Culture medium composition
WO2017112455A2 (en) * 2015-12-22 2017-06-29 Corning Incorporated Cell separation device and method for using same
TW201738256A (zh) * 2016-04-04 2017-11-01 日產化學工業股份有限公司 蛋白質產生方法
EP3591030A4 (de) * 2017-03-03 2020-03-25 FUJIFILM Corporation Zellkulturvorrichtung und zellkulturverfahren

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102575219A (zh) * 2009-08-26 2012-07-11 艾克塞勒雷克斯公司 连续回收收获袋
US20130081995A1 (en) * 2011-09-29 2013-04-04 Hyclone Laboratories, Inc. Filter systems for separating microcarriers from cell culture solutions
US20160251628A1 (en) * 2013-10-30 2016-09-01 Exmoor Pharma Concepts Ltd Apparatus And Method For Filtration Of A Suspension
WO2016002505A1 (ja) * 2014-06-30 2016-01-07 株式会社カネカ フィルター内の隆起部の外形を最適化したフィルター
JP2016010394A (ja) * 2014-06-30 2016-01-21 株式会社カネカ フィルター内の隆起部の比率を最適化したフィルター
WO2018123619A1 (ja) * 2016-12-26 2018-07-05 東洋製罐グループホールディングス株式会社 フィルタ付き細胞用バッグ、及びその製造方法

Also Published As

Publication number Publication date
TW202024314A (zh) 2020-07-01
EP3848451A4 (de) 2021-10-27
WO2020054755A1 (ja) 2020-03-19
US20220041971A1 (en) 2022-02-10
US20240132827A1 (en) 2024-04-25
EP3848451A1 (de) 2021-07-14
JP7501365B2 (ja) 2024-06-18
JPWO2020054755A1 (ja) 2021-08-30
US20240228935A9 (en) 2024-07-11

Similar Documents

Publication Publication Date Title
JP7501365B2 (ja) 分離デバイスおよびそれを用いて分離対象物を分離する方法
Feder et al. The large-scale cultivation of mammalian cells
TWI719697B (zh) 液體組成物
US9290731B2 (en) Filter apparatus and filter plate system
CN110475856B (zh) 使用纳米纤维的细胞培养
Reuveny Microcarrier culture systems
US20180291336A1 (en) Method of manufacturing and purifying exosomes from non-terminally differentiated cells
CN103476924A (zh) 收获细胞的方法和系统
JP2009247334A (ja) 細胞培養担体
WO2017175751A1 (ja) タンパク質産生方法
JP2019509047A (ja) 撹拌タンクバイオリアクタを用いた多能性幹細胞の増殖および継代
CN111139218A (zh) 用诱导多能干细胞或胚胎干细胞快速高效制备拟胚体的方法
CN104818238A (zh) 一种鸡肠上皮细胞分离培养方法
CN114807008B (zh) 一种番茄叶片原生质体单细胞悬液的制备方法及应用
CN107858322B (zh) 一种海马原代细胞培养体系的建立方法
US20220306980A1 (en) Cell recovery apparatus, cell recovery method, cell separation system, and cell separation method
KR101658529B1 (ko) 미세조류 배양 및 수확장치와 이를 이용한 이산화탄소 고정장치, 공기 또는 수질 정화장치
CN210340843U (zh) 贴壁细胞微载体培养装置
JP2021503908A (ja) 灌流バイオリアクターシステムおよび灌流細胞培養方法
WO2019108767A1 (en) Filtered cell culture caps and cell culture methods
CN115595288A (zh) 一种用于细胞培养的微载体及其制备方法
JPH07135963A (ja) 微生物の分離方法および微生物個体数の計測方法
CN109789350A (zh) 用于从细胞培养流体分离微载体的容器
EP4281536A1 (de) Verfahren zum wechseln des kulturmediums einer kultur mit hilfe von spinfiltern
WO2023228153A1 (en) High flow filter and method of use

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination