CN112970889A - Micromolecule peptide composition with anti-intoxication and sobering functions - Google Patents
Micromolecule peptide composition with anti-intoxication and sobering functions Download PDFInfo
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- CN112970889A CN112970889A CN202110209360.2A CN202110209360A CN112970889A CN 112970889 A CN112970889 A CN 112970889A CN 202110209360 A CN202110209360 A CN 202110209360A CN 112970889 A CN112970889 A CN 112970889A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/163—Liquid or semi-liquid tea extract preparations, e.g. gels, liquid extracts in solid capsules
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/30—Further treatment of dried tea extract; Preparations produced thereby, e.g. instant tea
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Abstract
The invention discloses a micromolecule peptide composition with an anti-intoxication and anti-hangover function, which comprises the following main components: mung bean peptide: 15% -30%, soybean peptide: 15% -30%, corn peptide: 20% -35%, oyster peptide: 5% -10%, green tea powder: 10% -20%, the mung bean peptide, the corn peptide and the soybean peptide all use self as raw materials, small molecular mung bean peptide, corn peptide and soybean peptide are obtained after water distribution, alcohol and sodium sulfite heating and stewing, filtering, enzymolysis, enzyme deactivation, decoloration and membrane separation are carried out, and finally obtained concentrated solution is dried, wherein the mung bean peptide, the corn peptide and the soybean peptide are extracts prepared from fresh green tea, and the green tea is prepared from the green tea through enzyme deactivation, stir-frying, grinding and stirring, and relates to the field of anti-alcoholism products. The small molecular peptide obtained by biological enzymolysis treatment directly enters small intestine to be absorbed without gastrointestinal secondary digestion, plays a role in tissue level, and the mung bean peptide, the soybean peptide and the corn peptide play a synergistic role to stably exist NAD + by improving the alanine and leucine content in blood, thereby realizing the functions of relieving alcoholism and preventing intoxication.
Description
Technical Field
The invention relates to the field of anti-inebriation products, in particular to a micromolecule peptide composition with an anti-inebriation and anti-inebriation function.
Background
With the improvement of the living standard of people, the consumption of wine is also increased, drinking in life, work and social intercourse is even an indispensable link, excessive drinking can cause alcoholism, the damage of systems such as central nerves, digestion, respiration, cardiovascular and cerebrovascular and the like caused by excessive drinking is very serious, the damage of the systems can cause damage to nerves and digestive systems, the development and research of foods capable of preventing and relieving alcoholism are concerned by more and more people, and in order to relieve or reduce the damage to bodies caused by drinking, various compositions are correspondingly developed by people so as to meet the requirements of drinkers on preventing and relieving alcoholism.
When drinking too much, ethanol entering a human body cannot be digested and absorbed, and flows into the brain along with blood, so that neuron cell membranes are damaged, and a central nervous system is weakened, so that the metabolism of the ethanol in the blood is accelerated, and the sobering up can be quicker.
Disclosure of Invention
The invention aims to: in order to solve the problems that the existing anti-inebriation product has toxic and side effects, the human body is easy to generate dependence, the safety is not high, and most of the existing anti-inebriation products have poor taste, the small molecular peptide composition with the anti-inebriation and sobering-up functions is provided.
In order to achieve the purpose, the invention provides the following technical scheme: a micromolecular peptide composition with anti-intoxication and anti-hangover functions comprises the following main components:
mung bean peptide: 15 to 30 percent of
Soybean peptide: 15 to 30 percent of
Corn peptide: 20 to 35 percent
Oyster peptide: 5 to 10 percent of
Green tea powder: 10 to 20 percent.
Preferably, the green tea powder is an extract prepared from fresh green tea, and is prepared by de-enzyming, parching, grinding and stirring green tea.
Preferably, the mung bean peptide, the corn peptide and the soybean peptide are prepared from raw materials of fresh shell-free oysters by the steps of preparing water, adding alcohol and sodium sulfite, heating, stewing, filtering, carrying out enzymolysis, inactivating enzyme, decoloring, carrying out membrane separation, and finally drying the obtained concentrated solution to obtain micromolecule mung bean peptide, corn peptide and soybean peptide, wherein the standard rod peptide chain of the mung bean peptide, the corn peptide and the soybean peptide is GIn-Leu-Leu-Pro-Phe, Lys-Leu-Pro-AIa-GIy-Thr-Leu-Phe, Phe-Phe-Leu-Ile-Tyr-Leu-Leu which mainly comprises 3-8 amino acids, mainly takes a peptide chain with the molecular weight of below 1000Dalton, and is dissolved in an acid-base environment of PH2-10, and the oyster peptide is prepared from fresh shell-free oysters by soaking, sterilizing and treating, The oyster peptide is obtained by enzymolysis, enzyme deactivation and centrifugal separation, and the benchmarking peptide chain of the oyster peptide is Tyr-Cys-Ser-Tyr-Thr-Met-Glu-Ala.
The green tea extract mainly comprises 3-8 amino acids, the peptide chain with the molecular weight below 1000Dalton is taken as the main material, compared with the original protein and the amino acids forming the original protein, the green tea extract has the advantages of good functionality, water solubility, water binding capacity and the like, can be completely dissolved under any acid-base condition of PH2-10, can be effectively combined with the green tea extract, and keeps the respective original physicochemical and nutritional characteristics.
Green tea powder obtained by the means of claim 2, green bean peptide, corn peptide and soybean peptide obtained by the means of claim 3, oyster peptide obtained by the means of claim 4;
s2: then pouring the green tea powder, the mung bean peptide, the corn peptide, the soybean peptide and the oyster peptide into a reactor according to a certain proportion, fully mixing, centrifuging, filtering, adsorbing, concentrating and drying to finally obtain the solid beverage or dissolving the solid beverage in water to prepare the solid beverage into a beverage which can be directly drunk, directly enter small intestine cells to absorb without secondary gastrointestinal digestion, play the role in the tissue level, is not influenced by the physical condition and is easier to absorb by the human body.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention is taken from natural food materials, is safe and reliable, is stable to heat and acid and alkali, has stable and non-lost function without influencing components, has wide biological effect, exerts synergistic effect on the mung bean peptide, the soybean peptide and the corn peptide, and realizes the functions of relieving alcoholism and preventing drunkenness by improving the content of alanine and leucine in blood and stably existing NAD +; in addition, the effects of relieving alcoholism and preventing drunkenness of the tea are recorded in the book ' compendium of materia medica ' Ohio ', tea polyphenol is the main active component of the tea, has good oxidation resistance, can effectively remove redundant free radicals and active oxygen in the body, can also reduce the content of malondialdehyde in blood plasma and inhibit the improvement of two enzyme activities of ALT and AST in blood serum, thereby protecting liver cells and accelerating the metabolism of ethanol, green tea is rich in high-activity tea polyphenol, and a small molecular peptide composition consisting of the tea polyphenol and 4 peptides can play the roles of relieving alcoholism and preventing drunkenness; compared with other anti-inebriation and anti-inebriation products, the anti-inebriation and anti-inebriation tea has the advantages that the anti-inebriation tea is mainly made of plant extracts, 4 peptides of the anti-inebriation tea are matched with green tea powder according to a certain proportion, supplement each other, the functions are remarkably improved, the production process is simple, the taste is fresh, and the anti-inebriation tea is beneficial to popularization and application;
2. the invention carries out biological enzymolysis treatment on food materials to obtain the small molecular peptides which can directly enter small intestines to be absorbed without secondary digestion in intestines and stomach, plays a role in the tissue level, the mung bean peptide, the soybean peptide and the corn peptide play a synergistic role, the NAD + stably exists by improving the alanine and leucine content in the blood to realize the functions of relieving alcoholism and preventing intoxication, after the ethanol enters the body, 90% is metabolized through oxidation in the liver, NAD + is needed to be used as coenzyme in the metabolic process, NAD + is sufficiently supplemented, tricarboxylic acid circulation is not limited any more, the increase of the concentration of ethanol in blood is prevented, oyster glycogen which is a component with the effects of dispelling the effects of alcohol and preventing intoxication in oyster peptide improves the activities of ADH and ALDH by 94.8% and 122.2% respectively within a certain time, so that the ethanol in the blood is metabolized more quickly, the concentration of the ethanol is obviously reduced, and the sobering effect is achieved.
Drawings
Fig. 1 is a graph of the anti-intoxication righting experimental results of the small molecule peptide composition of the present invention;
FIG. 2 is a graph showing the experimental results of the anti-intoxication sleep of the small molecule peptide composition of the present invention;
FIG. 3 is a diagram showing the experimental results of an anti-intoxication hangover sleep test using the small molecule peptide composition of the present invention;
FIG. 4 is a climbing experiment result chart of the anti-hangover effect of the small molecule peptide composition of the present invention.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
A micromolecular peptide composition with anti-intoxication and anti-hangover functions comprises the following main components:
mung bean peptide: 20 percent;
soybean peptide: 20 percent;
corn peptide: 30 percent;
oyster peptide: 10 percent;
green tea powder: 20 percent.
The green tea powder is an extract prepared from fresh green tea, and is prepared by green tea through deactivation of enzymes, stir-frying, grinding and stirring, the mung bean peptide, the corn peptide and the soybean peptide all use self as raw materials, the concentrated solution obtained is dried to obtain micromolecule mung bean peptide, corn peptide and soybean peptide through water distribution, alcohol and sodium sulfite heating and steaming, filtering, enzymolysis, enzyme deactivation, decoloration and membrane separation, the labeled rod peptide chain of the mung bean peptide, the corn peptide and the soybean peptide is GIn-Leu-Leu-Pro-Phe, Lys-Leu-Pro-AIa-GIy-Thr-Leu-Phe, Phe-Phe-Leu-Ile-Tyr-Leu-Leu, the labeled rod peptide chain of the mung bean peptide, the corn peptide and the soybean peptide mainly comprises 3-8 amino acids, the peptide chain with the molecular weight below 1000Dalton is taken as a main component, and the peptide chain is dissolved in the acid-alkali environment of PH2-10, the oyster peptide is prepared by taking fresh shell-free oysters as raw materials and carrying out soaking treatment, sterilization treatment, enzymolysis treatment, enzyme deactivation treatment and centrifugal separation, wherein a marker post peptide chain of the oyster peptide is Tyr-Cys-Ser-Tyr-Thr-Met-Glu-Ala, green tea powder, mung bean peptide, corn peptide, soybean peptide and oyster peptide are poured into a reactor according to a certain proportion, and after fully mixing, the mixture is centrifuged, filtered, adsorbed, concentrated and dried to finally obtain solid beverage or is dissolved in water to prepare the solid beverage for direct drinking.
The anti-intoxication effect of the small molecular peptide composition on experimental mice is researched by adopting a righting experiment (a normal saline-alcohol group and a composition-alcohol group, wherein each group comprises 27 mice) and an intoxication sleep experiment (a normal saline-alcohol group comprises 24 mice and a composition-alcohol group comprises 20 mice);
the hangover alleviating effect of the small molecular peptide composition on the acute drunken mice is researched by adopting drunken sleep experiments (16 mice in a normal saline group and 12 mice in a composition group) and climbing experiments (16 mice in a normal saline group and 12 mice in a composition group).
The wine, the composition and the normal saline are uniformly administrated by intragastric administration, the wine infusion amount is 15 mu L/g, and the composition infusion amount and the normal saline infusion amount are 5 mu L/g, so that: in the righting experiment of the anti-intoxication experiment, the intoxication rates of the normal saline liquor-adding group and the composition liquor-adding group are 88.89 percent and 33.56 percent in sequence;
in the drunk sleep experiment, the drunk rates of the normal saline wine adding group and the composition wine adding group are 100 percent and 40 percent in sequence, and the difference has statistical significance (P is less than 0.01).
In the drunk sleep experiment of the hangover alleviating experiment, the sleep duration time of the group of the wine and the normal saline and the group of the wine and the composition are (226.750 +/-115.090) min and (64.5 +/-31.274) min in sequence; in the climbing experiment, the sobering time of the group of the normal saline and the group of the composition sequentially has the statistical significance (P is less than 0.01) for (13973.380 +/-6764.977) s and (3170.330 +/-2526.911) s. And (4) conclusion: the small molecular peptide composition has certain effects of preventing and relieving acute drunkenness of mice.
SPF-grade KM mice, which are half male and half female, have the body mass of 25-45 g.
The data processing adopts SPSS17.0 data statistical software to carry out data analysis, the data analysis of counting data adopts chi-square test (if the expected count is less than 5, Fisher's precise test method is adopted) to measure data and express the data by mean standard deviation +/-s, the comparison between groups adopts t test, and the test level alpha is 0.05.
Randomly dividing the mice into two groups of normal saline wine adding group and composition wine adding group, wherein each group comprises 27 mice, determining that the wine filling amount is 15 mu L/g according to the pre-experiment before the experiment, the medicine filling amount is 5 mu L/g, the composition wine adding group is filled with gastric small molecular peptide composition solution 5 mu L/g, the normal saline wine adding group is filled with gastric normal saline with the same volume as the normal saline, and after 30min, filling the two groups with gastric white spirit, wherein each group is filled with 15 mu L/g; after each mouse finishes the gastric lavage, the mouse is placed upside down, namely the four limbs face upwards and the back faces downwards, the condition of righting reflex of the mouse is observed, righting reflex disappears and lasts for 30 seconds, and the mouse is drunk;
after drunk, the turning-over reflection is recovered, the turning-over time is less than 30s, and the sobering-up is realized, and the result is as follows: the drunkenness rate of the normal saline and the drunkenness group is 88.89 percent, and the drunkenness rate of the composition and the drunkenness group is 33.56 percent. Two comparisons, X2 ═ 7.477, P < 0.01, indicated: the small molecule peptide composition has an effect of preventing drunkenness in mice, as shown in fig. 1.
Randomly dividing the mice into two groups of a normal saline liquor group (24) and a composition liquor group (20), observing the reaction after the gastric lavage of each mouse is finished, taking the mouse with the indexes of back abdomen mopping, crawling instability, eye closure and laziness as sleep (drunk) indexes, and taking the flexible limbs, free movement and mental recovery as awakening (drunk) indexes; as a result: the drunkenness rate of the normal saline added group is 100 percent, the drunkenness rate of the composition added group is 40.00 percent, and the results show that when the two groups are compared, P is less than 0.01: the small molecule peptide composition has an effect of preventing drunkenness in mice, as shown in fig. 2.
The mice are randomly divided into two groups of a group (16 mice) of wine and normal saline and a group (12 mice) of composition, the mice are firstly filled with white spirit, after the mice are drunk by individuals, the mice of the group of wine and normal saline are filled with normal saline, the mice of the group of wine and composition are filled with small molecular peptide composition solution, the standard of the filling amount of the stomach is the same as the standard, the sleeping time of the mice is observed, and the result shows that: the small molecular peptide composition can effectively shorten the sleep duration of a mouse and has an anti-hangover effect on acute drunkenness of the mouse, as shown in fig. 3.
Grouping and gastric perfusion are the same as the drunk sleep experiment, a mouse is placed on a vertical metal net, the mouse climbing experiment condition is observed, and the sobering time is recorded, namely the climbing time from the drunk state to the sobering state, and the result shows that: the small molecular peptide composition can effectively shorten the sobering time of mice and has the sobering effect on acute drunkenness of the mice, as shown in figure 4.
The experiment shows that: the small molecular peptide composition has certain effects of preventing drunkenness and dispelling the effects of alcohol on an experimental mouse, the small molecular peptide obtained by carrying out biological enzymolysis on food materials is directly absorbed by small intestines without gastrointestinal secondary digestion, the effect is exerted on a tissue level, the mung bean peptide, the soybean peptide and the corn peptide exert a synergistic effect, and the effects of dispelling the effects of alcohol and preventing drunkenness are realized by stably existing NAD < + > through improving the content of alanine and leucine in blood. After the ethanol enters the body, 90% of the ethanol is metabolized through liver oxidation, NAD + is needed as a coenzyme in the metabolic process, the NAD + is sufficiently supplemented, the tricarboxylic acid cycle is not limited any more, and the increase of the concentration of the ethanol in the blood is prevented. Oyster peptide contains oyster glycogen which has the effects of relieving alcoholism and preventing drunkenness, and can respectively improve the activities of ADH and ALDH by 94.8 percent and 122.2 percent within a certain time, so that ethanol in blood is accelerated to metabolize, the concentration of the ethanol is obviously reduced, and the effect of sobering is achieved. In addition, the anti-alcohol and anti-intoxication effects of the tea are recorded in the book 'materia medica compendia leopard' as early as the records, and the tea polyphenol is a main active component of the tea, has good oxidation resistance, can effectively remove redundant free radicals and active oxygen in vivo, can also reduce the content of malondialdehyde in blood plasma, and inhibit the improvement of two enzyme activities of ALT and AST in blood serum, thereby protecting liver cells and accelerating the metabolism of ethanol. The green tea is rich in high-activity tea polyphenol, and a small molecular peptide composition consisting of the green tea and 4 peptides can play a role in dispelling the effects of alcohol and preventing drunkenness.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (5)
1. A micromolecular peptide composition with anti-intoxication and anti-hangover functions comprises the following main components:
mung bean peptide: 15 to 30 percent of
Soybean peptide: 15 to 30 percent of
Corn peptide: 20 to 35 percent
Oyster peptide: 5 to 10 percent of
Green tea powder: 10 to 20 percent.
2. The small molecule peptide composition with the function of preventing drunkenness according to claim 1, wherein: the green tea powder is an extract prepared from fresh green tea, and is prepared from green tea by deactivating enzymes, parching, grinding and stirring.
3. The small molecule peptide composition with the function of preventing drunkenness according to claim 1, wherein: the method comprises the following steps of taking mung bean peptide, corn peptide and soybean peptide as raw materials, preparing water, adding alcohol and sodium sulfite, heating, cooking, filtering, carrying out enzymolysis, inactivating enzyme, decolorizing, carrying out membrane separation, and finally drying the obtained concentrated solution to obtain micromolecule mung bean peptide, corn peptide and soybean peptide, wherein the benchmarking peptide chains of the mung bean peptide, the corn peptide and the soybean peptide are GIn-Leu-Leu-Pro-Phe, Lys-Leu-Pro-AIa-GIy-Thr-Leu-Phe, Phe-Phe-Leu-Ile-Tyr-Leu-Leu, the benchmarking peptide chains mainly comprise 3-8 amino acids, the peptide chain with the molecular weight below 1000Dalton is taken as a main component, and the peptide is dissolved in the environment of PH 2-10.
4. The small molecule peptide composition with the function of preventing drunkenness according to claim 1, wherein: the oyster peptide is prepared from fresh shell-free oysters by soaking, sterilizing, enzymolysis, enzyme deactivation and centrifugal separation, and the target rod peptide chain of the oyster peptide is Tyr-Cys-Ser-Tyr-Thr-Met-Glu-Ala.
5. The small molecule peptide composition with the function of preventing drunkenness and the preparation method thereof according to claim 1, comprises the following steps:
s1: green tea powder obtained by the means of claim 2, green bean peptide, corn peptide and soybean peptide obtained by the means of claim 3, oyster peptide obtained by the means of claim 4;
s2: then pouring the green tea powder, the mung bean peptide, the corn peptide, the soybean peptide and the oyster peptide into a reactor according to a certain proportion, fully mixing, centrifuging, filtering, adsorbing, concentrating and drying to finally obtain the solid beverage or dissolving the solid beverage in water to prepare the solid beverage into a beverage for direct drinking.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101618206A (en) * | 2009-07-24 | 2010-01-06 | 南方医科大学 | Composition for sobering up and protecting liver |
| CN101731630A (en) * | 2010-01-22 | 2010-06-16 | 完美（中国）日用品有限公司 | Health-care food with effects of neutralizing effect of alcoholic drinks and protecting liver |
| CN102727571A (en) * | 2012-07-23 | 2012-10-17 | 广东海洋大学 | Oyster and kudzu root natural anti-alcohol agent and preparation method thereof |
| CN108740679A (en) * | 2018-06-06 | 2018-11-06 | 唐山肽景堂生物科技有限公司 | A kind of solid beverage and preparation method thereof of antialcoholism peptide compounding |
| CN108813581A (en) * | 2018-05-18 | 2018-11-16 | 尚方堂中药有限公司 | A kind of liver-protecting sobering up composition and preparation method thereof |
-
2021
- 2021-02-25 CN CN202110209360.2A patent/CN112970889A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101618206A (en) * | 2009-07-24 | 2010-01-06 | 南方医科大学 | Composition for sobering up and protecting liver |
| CN101731630A (en) * | 2010-01-22 | 2010-06-16 | 完美（中国）日用品有限公司 | Health-care food with effects of neutralizing effect of alcoholic drinks and protecting liver |
| CN102727571A (en) * | 2012-07-23 | 2012-10-17 | 广东海洋大学 | Oyster and kudzu root natural anti-alcohol agent and preparation method thereof |
| CN108813581A (en) * | 2018-05-18 | 2018-11-16 | 尚方堂中药有限公司 | A kind of liver-protecting sobering up composition and preparation method thereof |
| CN108740679A (en) * | 2018-06-06 | 2018-11-06 | 唐山肽景堂生物科技有限公司 | A kind of solid beverage and preparation method thereof of antialcoholism peptide compounding |
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