CN112956590A - Recovery treatment method of traditional Chinese medicine waste residues, phellinus igniarius residue fermentation compound and application - Google Patents
Recovery treatment method of traditional Chinese medicine waste residues, phellinus igniarius residue fermentation compound and application Download PDFInfo
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Abstract
The invention discloses a recycling method of traditional Chinese medicine waste residues, a phellinus igniarius residue fermentation compound and application, wherein the recycling method of the traditional Chinese medicine waste residues comprises the following steps: (1) preparing a decoction dreg culture substrate: drying and crushing the traditional Chinese medicine waste residue, adding wheat bran, gypsum powder and quicklime, uniformly mixing, adding water for infiltration, bagging and sterilizing to obtain the residue culture medium; (2) activating aspergillus niger strains, inoculating the activated aspergillus niger strains on a dreg culture substrate, and performing primary fermentation; after the first fermentation is finished, activating the phellinus igniarius strain, inoculating the phellinus igniarius strain on the dregs of a decoction culture medium, and performing second fermentation to obtain a phellinus igniarius dregs of a decoction fermentation product; (3) drying the phellinus igniarius dregs fermentation product, and crushing to obtain the phellinus igniarius dregs fermentation compound. The invention recycles the traditional Chinese medicine waste residue into the feed additive by the bidirectional fermentation technology, accords with the current development trend of green and environmental protection, can realize the recycling of the traditional Chinese medicine waste residue and improve the economic benefit of traditional Chinese medicine production enterprises.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine waste residue treatment, in particular to a recovery treatment method of traditional Chinese medicine waste residue, a phellinus igniarius residue fermentation compound and application.
Background
In recent years, with the internationally increasing acceptance of traditional Chinese medicine and the strong support of the development of traditional Chinese medicine by the government of China, China gradually forms a large traditional Chinese medicine industry which is based on traditional Chinese medicine agriculture, mainly adopts the traditional Chinese medicine industry and is powered by the traditional Chinese medicine knowledge and economy industry. However, under the conditions of rapid growth and steady increase of yield of the traditional Chinese medicine industry, the traditional Chinese medicine solid waste brings huge pressure to the environment. At present, most of pharmaceutical factories mainly treat Chinese medicine residues by direct discharge, field accumulation, burying or combustion, or convert Chinese medicine residues into fuel gas by high-energy-consumption thermal cracking or gasification and other modes, so that the environment is polluted, and the great waste of Chinese medicine resources is caused.
In addition, limited by extraction and refining techniques, the utilization efficiency of traditional Chinese medicinal materials is low, the decoction dregs contain a large amount of nutrient substances such as crude protein, crude fat, crude fiber, vitamins and the like, and also contain various alkaloids, terpenes, flavonoids, quinones and other bioactive substances, and if the traditional Chinese medicine waste dregs are correctly recycled, the utilization value of the traditional Chinese medicine waste dregs can be greatly improved, so researchers in the field need to develop a method for performing high-value recycling treatment on the traditional Chinese medicine waste dregs to adapt to ever-expanding market demands.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention mainly aims to provide a method for recycling and treating the waste residue of the traditional Chinese medicine by adopting a bidirectional fermentation technology.
The invention also aims to provide the phellinus igniarius decoction dreg fermentation compound prepared by the method.
The invention further aims to provide application of the phellinus igniarius dreg fermentation compound as a feed additive in the field of feeds.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for recycling and treating traditional Chinese medicine waste residues comprises the following steps:
(1) preparing a decoction dreg culture substrate: drying and crushing the traditional Chinese medicine waste residue, adding wheat bran, gypsum powder and quicklime, uniformly mixing, adding water for infiltration, bagging and sterilizing to obtain the residue culture medium;
(2) activating aspergillus niger strains, inoculating the activated aspergillus niger strains on a dreg culture substrate, and performing primary fermentation; after the first fermentation is finished, activating the phellinus igniarius strain, inoculating the phellinus igniarius strain on the dregs of a decoction culture medium, and performing second fermentation to obtain a phellinus igniarius dregs of a decoction fermentation product;
(3) drying the phellinus igniarius dregs fermentation product, and crushing to obtain the phellinus igniarius dregs fermentation compound.
Further, the traditional Chinese medicine waste residue in the step (1) is compound Ganmaoling tablet dregs; the residue of the compound GANMAOLING tablet comprises flos Lonicerae, herba Ponciri Trifoliatae, flos Chrysanthemi Indici, thin Evodia, radix Isatidis, and flos Ilicis Asprellae.
Further, the drying in the step (1) can be performed by air drying, sun drying or oven drying.
Further, the mass ratio of the traditional Chinese medicine waste residue, the wheat bran, the gypsum powder and the quick lime in the step (1) is (80-85): (15-20): (1-2):(1-2).
Further, the step (1) of adding water for infiltration is to control the total water content of the decoction dreg culture substrate to be 50-60%.
Further, the packaging in the step (1) adopts polypropylene or polyethylene plastic bags, and the weight of the culture medium of the herb residue in each plastic bag is 1kg-1.5 kg.
Further, the sterilization in the step (1) can adopt high pressure sterilization or normal pressure sterilization. The autoclaving is carried out at 1.4MP for 2.5-3 h; the normal pressure sterilization is carried out for 12-16h at 100 ℃.
Further, the activation of the aspergillus niger species in the step (2) specifically comprises the following steps: inoculating Aspergillus niger seed to potato glucose agar culture medium for activation culture, culturing at 28-32 deg.C in constant temperature incubator for 2-3d, eluting with appropriate amount of sterile ultrapure water to obtain spore suspension, transferring into 100mL liquid potato culture medium, and adjusting concentration to 1 × 107And culturing the strain per mL by shaking at the temperature of 28-32 ℃ in a constant-temperature shaking culture box at the speed of 100-.
Furthermore, the inoculation amount of the aspergillus niger bacterial liquid in the drug-property culture medium is 8-12%.
Further, the fermentation temperature of the first fermentation in the step (2) is 28-32 ℃, and the fermentation time is 2-3 d.
Further, the phellinus linteus strain in the step (2) is phellinus linteus strain (s.
Further, in the step (2), the activation of the phellinus igniarius strain specifically comprises the following steps: inoculating Phellinus Linteus mother strain to potato glucose agar culture medium, activating at 23-28 deg.C for 8-10 days to obtain Phellinus Linteus block, eluting with sterile ultrapure water to obtain spore suspension, transferring into 100mL liquid potato culture medium, and adjusting concentration to 1 × 107Culturing the strain per mL with a constant-temperature shaking culture box at 23-28 ℃ for 10-15d at 90-110r/min by shaking to obtain phellinus igniarius bacterial liquid.
Furthermore, the inoculation amount of the phellinus igniarius bacterial liquid in the drug-property culture medium is 8-12%.
Further, the fermentation temperature of the second fermentation in the step (2) is 23-28 ℃, and the fermentation time is 7-14 d.
Further, the drying in the step (3) can be carried out by drying, and the water content of the fermentation product of the phellinus igniarius dregs is controlled to be 18-22%.
Further, the crushing in the step (3) is to crush the fermentation product of phellinus igniarius dregs to the granularity of 2.0-3.0 mm.
The invention also provides a phellinus igniarius dreg fermentation compound prepared by the method, and the compound is prepared by taking the traditional Chinese medicine waste residues as a medicine property matrix and performing combined fermentation on aspergillus niger and phellinus igniarius.
The invention also provides application of the phellinus igniarius decoction dreg fermentation compound in the field of feed, when the phellinus igniarius decoction dreg fermentation compound is used as a feed additive for pork pigs and poultry, the addition amount of the feed additive is 1% -5% of the feed content, and the phellinus igniarius decoction dreg fermentation compound can be used as a feed additive for improving the resistance of the pork pigs or poultry to swine flu and avian flu.
Compared with the prior art, the invention has the following advantages and technical effects:
(1) according to the invention, a bidirectional fermentation technology is adopted to recover and treat the traditional Chinese medicine waste residue, aspergillus niger and phellinus igniarius are taken as fermentation strains, the traditional Chinese medicine waste residue is taken as a medicine property matrix, the traditional Chinese medicine waste residue can provide nutrition required by growth of the aspergillus niger and phellinus igniarius, enzyme generated by growth of the aspergillus niger and phellinus igniarius promotes effective components in the traditional Chinese medicine waste residue to be separated out, the medicine effect is enhanced, and meanwhile, crude fibers of the traditional Chinese medicine waste residue after fermentation treatment can loosen a cellulose structure under the action of the enzyme, so that the palatability is improved, and the. The method provided by the invention has the advantages that the traditional Chinese medicine waste residue is recycled by the bidirectional fermentation technology, the current green and environment-friendly development trend is met, the traditional Chinese medicine waste residue can be recycled, and the economic benefit of traditional Chinese medicine production enterprises is improved.
(2) According to the invention, aspergillus niger and phellinus igniarius are combined to carry out multiple fermentation treatment on traditional Chinese medicine waste residues, most of the traditional Chinese medicine materials are used as medicines at the root part in the compound Ganmaoling tablet medicine residues, the crude fibers are more, aspergillus niger can generate abundant cellulase and pectinase, the crude fibers in the medicine residues can be degraded, the separation of the effective components of the traditional Chinese medicine residues is facilitated, the cellulose components are degraded into easily-utilized micromolecular carbohydrate substances, nutrient substances can be provided for the secondary fermentation of medicinal fungi phellinus igniarius, and the development and growth of phellinus igniarius mycelia are facilitated.
(3) According to the invention, phellinus igniarius is adopted to ferment traditional Chinese medicine waste residues, active ingredients of the morin and the polyphenol compounds (such as Hispidin) contained in fermentation liquor of the phellinus igniarius have the effect of inhibiting the activity of influenza virus neuraminidase, the phellinus igniarius can synergize with active substances in compound cold tablet medicine residues, the phellinus igniarius can modify and convert the active substances in the compound cold tablet medicine residues in the fermentation process, the anti-influenza effect of fermentation products of the phellinus igniarius medicine residues is further improved, the phellinus igniarius is used as a feed additive, the anti-influenza virus capability of pork pigs and poultry can be improved, the phellinus igniarius can be replaced for use, and meanwhile, the nutrient substances in the medicine residues and the phellinus igniarius can also promote the growth and development of.
(4) In the invention, after the waste residue of traditional Chinese medicine is crushed, wheat bran, gypsum powder and quicklime are added as auxiliary materials to be mixed to prepare a medicinal culture matrix, wherein the wheat bran can provide organic nitrogen sources such as amino acid and the like and growth factors in the fermentation process, and the wheat bran can loosen the matrix to increase ventilation; the gypsum powder and the quick lime are added to provide calcium and trace elements for the medicinal culture medium, the pH drop of the medicinal culture medium can be buffered, and the medicinal culture medium is more suitable for the growth and development of aspergillus niger and phellinus igniarius under the specific proportion of the traditional Chinese medicine waste residues and the auxiliary materials, and is more favorable for the fermentation process.
Detailed Description
The present invention will be described in further detail with reference to specific examples, but the embodiments of the present invention are not limited thereto. All the raw materials and reagents used in the present invention are commercially available raw materials and reagents, unless otherwise specified. In the examples, the components are used in g and mL in parts by mass.
Example 1
(1) Preparing a decoction dreg culture substrate: drying and crushing 80 parts by mass of compound Ganmaoling tablet dregs (honeysuckle, citrus unshiu, wild chrysanthemum, bitter orange, south isatis root and holly), adding 18 parts by mass of wheat bran, 1 part by mass of gypsum powder and 1 part by mass of quick lime, uniformly mixing, adding water to soak the dregs to ensure that the water content of the dregs culture medium is 50%, then bagging with a polyethylene plastic bag (1 kg/bag), keeping 1.4MP for 2.5h and sterilizing to obtain the dregs culture medium;
(2) inoculating Aspergillus niger mother strain to potato glucose agar culture medium, activating, culturing at 28 deg.C for 3d, eluting with sterile ultrapure water to obtain spore suspension, transferring into 100mL liquid potato culture medium, and adjusting concentration to 1 × 107Performing shaking culture at 100r/min for 1d at 28 ℃ in a constant-temperature shaking culture box to obtain Aspergillus niger liquid, inoculating the Aspergillus niger liquid on a dreg culture substrate, performing first fermentation, wherein the inoculum size is 8%, the fermentation temperature is 28 ℃, and the fermentation time is 3 d;
inoculating Phellinus Linteus mother strain to potato glucose agar culture medium, activating at 28 deg.C for 8 days to obtain Phellinus Linteus block, eluting with appropriate amount of sterile ultrapure water to obtain spore suspension, transferring into 100mL liquid potato culture medium, and adjusting concentration to 1 × 107Performing shake culture with a constant-temperature shake culture box at 28 ℃ for 10d at 90r/min to obtain phellinus igniarius bacterial liquid, inoculating the phellinus igniarius bacterial liquid on a residue culture substrate which is subjected to primary fermentation, performing secondary fermentation, wherein the inoculation amount is 10%, the fermentation temperature is 28 ℃, and the fermentation time is 10d to obtain phellinus igniarius residue fermentation products;
(3) drying the fermentation product of the phellinus igniarius dregs, and crushing the phellinus igniarius dregs into powder with the granularity of 3.0mm to obtain the phellinus igniarius dregs fermentation compound.
Example 2
(1) Preparing a decoction dreg culture substrate: drying and crushing 85 parts by mass of compound Ganmaoling tablet dregs (honeysuckle, citrus unshiu, wild chrysanthemum, bitter orange, south isatis root and holly), adding 15 parts by mass of wheat bran, 2 parts by mass of gypsum powder and 2 parts by mass of quick lime, uniformly mixing, adding water to soak the dregs culture medium until the water content is 60%, then bagging with a polyethylene plastic bag (1.5 kg/bag), keeping 1.4MP for 3 hours and sterilizing to obtain the dregs culture medium;
(2) inoculating Aspergillus niger seed to potato glucose agar culture medium for activation culture, culturing at 32 deg.C in constant temperature incubator for 2 days, and activatingEluting Aspergillus niger with appropriate amount of sterile ultrapure water to obtain spore suspension, transferring into 100mL liquid potato culture medium, and adjusting concentration to 1 × 107Performing shaking culture at 100r/min for 0.5d at 32 ℃ in a constant-temperature shaking culture box to obtain Aspergillus niger liquid, inoculating the Aspergillus niger liquid on a dreg culture substrate, performing primary fermentation, wherein the inoculation amount is 12%, the fermentation temperature is 32 ℃, and the fermentation time is 2 d;
inoculating Phellinus Linteus mother strain to potato glucose agar culture medium, activating at 23 deg.C for 10 days to obtain Phellinus Linteus block, eluting with appropriate amount of sterile ultrapure water to obtain spore suspension, transferring into 100mL liquid potato culture medium, and adjusting concentration to 1 × 107Performing shake culture with a constant-temperature shake culture box at 23 ℃ for 15d at 110r/min to obtain phellinus igniarius bacterial liquid, inoculating the phellinus igniarius bacterial liquid on a decoction dreg culture substrate which is subjected to primary fermentation, performing secondary fermentation, wherein the inoculation amount is 8%, the fermentation temperature is 23 ℃, and the fermentation time is 14d to obtain phellinus igniarius dreg fermentation products;
(3) drying the fermentation product of the phellinus igniarius dregs, and crushing the phellinus igniarius dregs into powder with the granularity of 2.5mm to obtain the phellinus igniarius dregs fermentation compound.
Example 3
(1) Preparing a decoction dreg culture substrate: drying and crushing 82 parts by mass of compound Ganmaoling tablet dregs (honeysuckle, citrus unshiu, wild chrysanthemum, bitter orange, south isatis root and holly), adding 20 parts by mass of wheat bran, 1 part by mass of gypsum powder and 2 parts by mass of quick lime, uniformly mixing, adding water to soak until the water content of the dregs culture medium is 55%, then bagging with a polyethylene plastic bag (1.2 kg/bag), keeping the temperature at 100 ℃ for 12 hours and sterilizing to obtain the dregs culture medium;
(2) inoculating Aspergillus niger seed to potato glucose agar culture medium for activation culture, culturing at 30 deg.C in constant temperature incubator for 2.5d, eluting with appropriate amount of sterile ultrapure water to obtain spore suspension, transferring into 100mL liquid potato culture medium, and adjusting concentration to 1 × 107Culturing in a constant temperature shaking incubator at 30 deg.C for 1d at 150r/minObtaining Aspergillus niger liquid, inoculating the Aspergillus niger liquid on the dreg culture medium, and performing primary fermentation, wherein the inoculation amount is 10%, the fermentation temperature is 30 ℃, and the fermentation time is 2.5 days;
inoculating Phellinus Linteus mother strain to potato glucose agar culture medium, activating at 25 deg.C for 9 days to obtain Phellinus Linteus block, eluting with appropriate amount of sterile ultrapure water to obtain spore suspension, transferring into 100mL liquid potato culture medium, and adjusting concentration to 1 × 107Performing shake culture with a constant-temperature shake culture box at 25 ℃ for 12d at 100r/min to obtain phellinus igniarius bacterial liquid, inoculating the phellinus igniarius bacterial liquid on a residue culture substrate which is subjected to primary fermentation, performing secondary fermentation, wherein the inoculation amount is 12%, the fermentation temperature is 25 ℃, and the fermentation time is 7d to obtain phellinus igniarius residue fermentation products;
(3) drying the fermentation product of the phellinus igniarius dregs, and crushing the phellinus igniarius dregs into powder with the granularity of 2.5mm to obtain the phellinus igniarius dregs fermentation compound.
Comparative example 1
(1) Preparing a decoction dreg culture substrate: drying and crushing 80 parts by mass of compound Ganmaoling tablet dregs (honeysuckle, citrus unshiu, wild chrysanthemum, bitter orange, south isatis root and holly), adding 18 parts by mass of wheat bran, 1 part by mass of gypsum powder and 1 part by mass of quick lime, uniformly mixing, adding water to soak the dregs culture medium until the water content is 50%, then bagging with a polyethylene plastic bag (1 kg/bag), keeping 1.4MP for 2.5h and sterilizing to obtain the dregs culture medium;
(2) inoculating Aspergillus niger mother strain to potato glucose agar culture medium, activating, culturing at 28 deg.C for 3d, eluting with sterile ultrapure water to obtain spore suspension, transferring into 100mL liquid potato culture medium, and adjusting concentration to 1 × 107Performing shaking culture at 100r/min for 1d at 28 ℃ in a constant-temperature shaking culture box to obtain Aspergillus niger liquid, inoculating the Aspergillus niger liquid onto the residue culture medium, and fermenting at the inoculation amount of 8%, the fermentation temperature of 28 ℃ and the fermentation time of 3d to obtain residue fermentation product;
(3) drying the residue fermentation product, and pulverizing to 3.0mm to obtain residue fermentation compound.
Comparative example 2
(1) Preparing a decoction dreg culture substrate: drying and crushing 80 parts by mass of compound Ganmaoling tablet dregs (honeysuckle, citrus unshiu, wild chrysanthemum, bitter orange, south isatis root and holly), adding 18 parts by mass of wheat bran, 1 part by mass of gypsum powder and 1 part by mass of quick lime, uniformly mixing, adding water to soak the dregs culture medium until the water content is 50%, then bagging with a polyethylene plastic bag (1 kg/bag), keeping 1.4MP for 2.5h and sterilizing to obtain the dregs culture medium;
(2) inoculating Phellinus Linteus mother strain to potato glucose agar culture medium, activating at 28 deg.C for 8 days to obtain Phellinus Linteus block, eluting with appropriate amount of sterile ultrapure water to obtain spore suspension, transferring into 100mL liquid potato culture medium, and adjusting concentration to 1 × 107Performing shake culture with a constant-temperature shake culture box at 28 ℃ for 10d at 90r/min to obtain phellinus igniarius bacterial liquid, inoculating the phellinus igniarius bacterial liquid on a dreg culture substrate, and fermenting, wherein the inoculation amount is 10%, the fermentation temperature is 28 ℃, and the fermentation time is 10d to obtain dreg fermentation products;
(3) drying the fermentation product of Phellinus Linteus residue, and pulverizing to 3.0mm to obtain residue fermentation compound.
Comparative example 3
Drying 80 parts by mass of compound GANMAOLING tablet residues (flos Lonicerae, mandarin orange, flos Chrysanthemi Indici, thin Evodia, radix Isatidis, and flos Ilicis Asprellae), adding 18 parts by mass of wheat bran, 1 part by mass of Gypsum Fibrosum powder, and 1 part by mass of calx, mixing, and pulverizing to obtain residue mixture with particle size of 3.0 mm.
Animal testing
The samples prepared in examples 1-3 and comparative examples 1-3 were used as feed additives (added in an amount of 3%) in broiler feeding, and the influence of each sample on the productivity and immunity of broilers was examined.
Design of experiments
Selecting 300 healthy broiler chickens of 1 day old with similar body conditionsThe results were divided into six groups of 50 animals each, namely, example 1 group, example 2 group, example 3 group, comparative example 1 group, comparative example 2 group, comparative example 3 group and blank group, wherein 3% of feed additive (sample prepared in each group) was added to the basic ration in the example group and the comparative example group, and the feed was continuously fed for 8 weeks twice a day, and the feed was fed in the morning 6: 00 and 17 pm: 00, all animals can drink water freely and move freely, and the breeding density is 8 animals/m2。
The weight was measured at 1 day, 4 weeks and 8 weeks, and the results are shown in Table 1.
Avian influenza vaccines (H5 subtype) were respectively administered to chicks at 10 days of age, and blood samples were collected at 10 days of age (before immunization), 3 weeks of age, and 6 weeks of age, respectively, by the Nannong David Co., Ltd., Guangzhou city, and the titer of H5 antibody in serum was determined by hemagglutination inhibition assay, with specific results as shown in Table 2.
TABLE 1 influence of the feed additives of each group on the weight gain of the chicks/g
| Group of | Age of 1 day | 4 weeks old | 8 weeks old |
| EXAMPLE 1 group | 42.89±0.53 | 633.54±12.98** | 1467.43±20.37** |
| EXAMPLE 2 group | 43.23±0.25 | 645.12±13.32** | 1454.98±18.44** |
| EXAMPLE 3 group | 43.04±0.31 | 649.76±10.24** | 1474.26±18.82** |
| Comparative example 1 group | 42.96±0.45 | 608.32±14.54△ | 1325.45±21.35△ |
| Comparative example 2 group | 43.32±0.19 | 612.34±12.32△ | 1343.89±20.71△ |
| Comparative example 3 group | 43.22±0.17 | 592.56±9.45 | 1296.82±16.63 |
| Blank group | 43.15±0.62 | 583.56±8.87 | 1265.54±15.32 |
Remarking: p < 0.5; p < 0.01; Δ is P < 0.5; Δ is P < 0.01.
As can be seen from the results in table 1, compared with the blank group, the weight of the chicks of the groups of examples 1 to 3 at 4 weeks of age and 8 weeks of age is significantly different from that of the blank group, which indicates that the phellinus igniarius residue fermentation compound prepared by performing bidirectional fermentation on the residues of the compound Ganmaoling tablets has significantly improved growth performance on broilers and can be used as a feed additive; the medicine residues in the comparative example 1 are fermented independently by aspergillus niger, the medicine residues in the comparative example 2 are fermented independently by phellinus igniarius, and the weight increase of the broiler chickens in the comparative example 1 and the comparative example 2 is remarkably different from that in the example 1, which shows that the combined fermentation of the aspergillus niger and the phellinus igniarius on the medicine residues obviously improves the nutritional performance of the compound Ganmaoling tablet medicine residues; the medicine dregs of the comparative example 3 are only subjected to simple drying and crushing treatment, and the weight of the group of chicks has no significant difference from that of the blank group of chicks.
TABLE 2 Effect of various groups of feed additives on the titer of avian influenza H5 antibody in broiler serum
| Group of | Age of 10 days | 3 weeks old | 6 weeks old |
| EXAMPLE 1 group | 2.4±0.3 | 7.4±0.4** | 6.3±0.2* |
| EXAMPLE 2 group | 2.3±0.2 | 7.7±0.3** | 6.8±0.2* |
| EXAMPLE 3 group | 2.4±0.5 | 7.2±0.2** | 6.7±0.3* |
| Comparative example 1 group | 2.2±0.4 | 5.6±0.5△△ | 5.1±0.4△ |
| Comparative example 2 group | 2.3±0.3 | 5.8±0.3△△ | 5.4±0.5△ |
| Comparative example 3 group | 2.1±0.2 | 5.3±0.2 | 5.0±0.4 |
| Blank group | 2.4±0.3 | 4.9±0.3 | 4.5±0.3 |
Remarking: p < 0.5; p < 0.01; Δ is P < 0.5; Δ is P < 0.01.
The results in table 2 show that compared with the blank control group, the avian influenza H5 subtype antibody titers of the groups in examples 1 to 3 are remarkably improved, which indicates that the Phellinus linteus residue fermentation compound obtained by performing bidirectional fermentation on the residues of the compound Ganmaoling tablets by virtue of Aspergillus niger and Phellinus linteus is beneficial to improving the immunity and anti-influenza virus capability of broiler chickens; the aspergillus niger used alone in the group of the comparative example 1 and the phellinus igniarius used alone in the group of the comparative example 2 are fermented, and the antibody titer in the serum of the two groups is poor in significance compared with that of the group of the example 2, which shows that the combined fermentation can improve the immunity enhancing performance of the fermentation product; the herb residues in the group of the comparative example 3 are not fermented, and the immune enhancing effect of the herb residues is not significantly different from that of the blank group.
The above embodiments are the best mode for carrying out the present invention, but the embodiments of the present invention are not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be regarded as equivalent substitutions and are included in the scope of the present invention.
Claims (10)
1. A method for recycling and treating traditional Chinese medicine waste residues is characterized by comprising the following steps:
(1) preparing a decoction dreg culture substrate: drying and crushing the traditional Chinese medicine waste residue, adding wheat bran, gypsum powder and quicklime, uniformly mixing, adding water for infiltration, bagging and sterilizing to obtain the residue culture medium;
(2) activating aspergillus niger strains, inoculating the activated aspergillus niger strains on a dreg culture substrate, and performing primary fermentation; after the first fermentation is finished, activating the phellinus igniarius strain, inoculating the phellinus igniarius strain on the dregs of a decoction culture medium, and performing second fermentation to obtain a phellinus igniarius dregs of a decoction fermentation product;
(3) drying the phellinus igniarius dregs fermentation product, and crushing to obtain the phellinus igniarius dregs fermentation compound.
2. The method for recycling and treating the waste residues of the traditional Chinese medicine according to claim 1, which is characterized in that: the traditional Chinese medicine waste residue is compound cold tablet dregs; the residue of the compound GANMAOLING tablet comprises flos Lonicerae, herba Ponciri Trifoliatae, flos Chrysanthemi Indici, thin Evodia, radix Isatidis, and flos Ilicis Asprellae.
3. The method for recycling and treating the waste residues of the traditional Chinese medicine according to claim 1, which is characterized in that: the mass ratio of the traditional Chinese medicine waste residue, the wheat bran, the gypsum powder and the quicklime in the step (1) is (80-85): (15-20): (1-2):(1-2).
4. The method for recycling and treating the waste residues of the traditional Chinese medicine according to claim 1, which is characterized in that: in the step (1), the water is added for infiltration, so that the total water content of the decoction dreg culture substrate is controlled to be 50-60%.
5. The method for recycling and treating the waste residues of the traditional Chinese medicine according to claim 1, which is characterized in that: the fermentation temperature of the first fermentation in the step (2) is 28-32 ℃, and the fermentation time is 2-3 d.
6. The method for recycling and treating the waste residues of the traditional Chinese medicine according to claim 1, which is characterized in that: the fermentation temperature of the second fermentation in the step (2) is 23-28 ℃, and the fermentation time is 7-14 days.
7. The method for recycling and treating the waste residues of the traditional Chinese medicine according to claim 1, which is characterized in that: in the step (3), the fermentation product of the phellinus igniarius dregs is crushed to the granularity of 2.0-3.0 mm.
8. A phellinus igniarius dregs fermented compound, which is characterized by being prepared according to the method for recovering and treating the traditional Chinese medicine dregs in any one of claims 1 to 7.
9. The use of phellinus igniarius dreg fermentation compound according to claim 8 in the field of feed.
10. The application of the phellinus igniarius dreg fermentation compound in the field of feed according to claim 9 is characterized in that: when the phellinus igniarius dreg fermentation compound is used as a feed additive, the addition amount of the feed additive is 1% -5% of the feed content.
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