CN112852621A

CN112852621A - Production integration system of ginseng ferment, method for producing ginseng ferment by using same, ginseng ferment and application thereof

Info

Publication number: CN112852621A
Application number: CN202110081151.4A
Authority: CN
Inventors: 陈贤; 曹华; 成志玲; 周永传; 卢玲巧
Original assignee: Ganeng Biotechnology Shanghai Co ltd
Current assignee: Ganeng Biotechnology Shanghai Co ltd
Priority date: 2021-01-21
Filing date: 2021-01-21
Publication date: 2021-05-28
Anticipated expiration: 2041-01-21
Also published as: CN112852621B

Abstract

The application relates to the technical field of daily chemical raw material preparation, and specifically discloses a ginseng ferment production integration system, a method for producing ginseng ferment by using the same, the ginseng ferment and application thereof. The production integrated system of the ginseng ferment comprises an ethanol heating storage tank, a condensate storage tank, an extraction tank, a hot water recovery tank, a fermentation tank, a condenser, a cooler, an enzyme mixing tank, a heater, a gas-liquid separator and an alcohol-water mixing tank. By adopting the differential gradient extraction, extraction and concentration and fermentation integrated technology, the defects of low efficiency, long period, high energy and material consumption and poor batch stability of the traditional preparation process of the ginseng ferment are overcome.

Description

Production integration system of ginseng ferment, method for producing ginseng ferment by using same, ginseng ferment and application thereof

Technical Field

The application relates to the technical field of daily chemical raw material preparation, in particular to a ginseng ferment production integrated system, a method for producing the ginseng ferment by using the ginseng ferment production integrated system and application of the ginseng ferment.

Background

Ginseng has been known for at least 4000 years, is listed as the superior in Shen nong Ben Cao Jing of east Han (25-220 A.D.), and 21 prescriptions with compatible Ginseng are collected in Shang Han Zhong Jing (treatise on Cold-induced diseases), and later generations of Ben Cao are collected in Ming Yi Bie Lu (miscellaneous records of famous physicians), Ben Cao Tu Jing (illustrated Manual of materia Medica), Ben Cao gang mu, etc. Li Shizhen discusses ginseng in compendium of materia Medica for the first time, and it is considered that ginseng is a miraculous drug covering all diseases and can cure all deficiency syndromes of both men and women. The title of the king of all grass and the first of all the medicines is obtained

The ginseng has multiple functions and obvious curative effect, so that the ginseng becomes an important raw material for industries such as medicine industry, health care products, cosmetics and the like. Preliminary statistical results show that the traditional Chinese medicine containing ginseng reaches more than 370, and ginseng is one of the most widely applied traditional Chinese medicinal materials in China. 1200 Chinese medicinal materials are commonly used in China, and the yield value exceeds about 50 more than one hundred million yuan and about 10 more than 5 hundred million yuan. The ginseng has a yield value of 20 billion yuan, and is one of the Chinese medicinal material varieties with the highest yield value.

However, the main application of ginseng, both ancient prescription and modern preparation, is concentrated on oral administration, and the development of ginseng products for external use, especially in the field of daily chemicals, is mainly claimed at present, and the effect is rarely really generated. The main reason is that the ginsenoside which is the main component of the ginseng is metabolized by microorganisms in the gastrointestinal tract before being absorbed in a human body, and then is absorbed and acts by the human body, and no intestinal microorganisms participate in the metabolism on the skin, so the bioavailability of the externally applied ginseng is low.

In order to increase the bioavailability of the active ingredients of ginseng on the skin, ginseng is fermented to prepare ginseng ferment which can be directly utilized by the skin, which is a better choice.

However, the existing ginseng ferment has low preparation efficiency and large batch-to-batch difference, so that the improvement of the preparation efficiency of the ginseng ferment has practical guiding significance for the application of ginseng, particularly in the field of daily chemicals.

Disclosure of Invention

In order to improve the preparation efficiency of ginseng ferment and reduce the difference between different batches, the application provides a production integrated system of ginseng ferment, utilize it to carry out the method of ginseng ferment production and the application of ginseng ferment.

First aspect, the application provides a production integrated system of ginseng ferment adopts following technical scheme:

a production integrated system of ginseng ferment comprises an ethanol heating storage tank, a condensate storage tank, an extraction tank, a hot water recovery tank, a fermentation tank, a condenser, a cooler, an enzyme mixing tank, a heater, a gas-liquid separator and an alcohol-water mixing tank;

an enzyme mixed liquid inlet pipeline and an alcohol-water mixed liquid inlet pipeline are arranged at the top of the extraction tank, an extract reflux inlet pipeline is arranged on the side wall of the extraction tank, and an extract outlet is arranged at the bottom of the extraction tank;

a filter screen is arranged at the lower part in the extraction tank, and the aperture of the filter screen is 40-60 meshes;

the bottom outlet of the enzyme mixing tank is connected with an enzyme mixed liquid inlet pipeline arranged at the top of the extraction tank through a valve F1 by a pipeline;

the top of the alcohol-water mixing tank is provided with an alcohol inlet pipeline and a water inlet pipeline, and the bottom of the alcohol-water mixing tank is provided with an alcohol-water mixed liquid outlet;

an outlet at the bottom of the alcohol-water mixing tank is connected with an alcohol-water mixed liquid inlet pipeline at the top of the extraction tank through a pipeline;

an outlet at the bottom of the ethanol heating storage tank is connected with an ethanol inlet pipeline at the top of the ethanol-water mixing tank through a valve F2, a first delivery pump and a valve F3;

an outlet at the bottom of the condensate storage tank is connected with an ethanol inlet pipeline at the top of the alcohol-water mixing tank through a valve F4, a second delivery pump and a valve F5; a pipeline bypass is arranged on the pipeline where the second conveying pump is located, a valve F6 is arranged on the pipeline bypass, and the valve F6 is located between the valve F4 and the valve F5;

the bottom of the extraction tank is divided into two paths through a valve F7, a third delivery pump and a pipeline filter, and the first path is connected with a reflux inlet pipeline on the side wall of the extraction tank after passing through a valve F8; the second path is connected with an inlet pipeline arranged at the bottom of the heater after passing through a valve F9, an outlet pipeline at the top of the heater tangentially enters a gas-liquid separator, and a top outlet of the gas-liquid separator is connected with an inlet pipeline arranged at the top of the condenser through a pipeline;

the top of the fermentation tank is provided with a water inlet pipeline which is communicated with a valve F14;

after the extracting solution from the outlet of the extracting tank reaches the concentration, the extracting solution passes through a valve F10 and a fifth delivery pump and then enters the material pipeline inlet of the cooler through a pipeline, and the material outlet pipeline of the cooler is combined with a valve F14 and a pipeline between water inlets arranged at the top of the fermentation tank;

the filtering pore diameter of the pipeline filter is 100-200 nm;

the bottom outlet pipeline of the gas-liquid separator is merged with a pipeline between a bottom outlet valve F7 of the extraction tank and the third delivery pump after passing through a valve F11;

the condenser is also provided with a cooling water inlet and a cooling water outlet, and the cooling water outlet is connected with a water inlet pipeline at the top of the fermentation tank and a pipeline between the valves F14 after passing through a valve F12 and a valve F13 through pipelines;

a branch pipeline is arranged between a cooling water outlet of the condenser and a valve F12 and is connected with a water inlet pipeline at the top of the alcohol-water mixing tank after passing through a valve F15;

a branch pipeline is arranged on a pipeline between the valve F12 and the valve F13 and is connected with a cooling water inlet pipeline arranged at the bottom of the cooler through a valve F16, and a cooling water outlet arranged at the top of the cooler is connected with a water inlet pipeline arranged at the top of the hot water recovery tank through a valve F19;

the bottom outlet of the hot water recovery tank is divided into two paths after passing through a valve F23 and a fourth delivery pump, the first path enters a fermentation tank jacket through a valve F24, the top of the fermentation tank jacket is provided with an outlet and is connected with a return pipe inlet arranged at the top of the hot water recovery tank through a pipeline; the second path is merged with a cooling water inlet pipeline of the cooler through a valve F18 and a valve F17 and enters the cooler;

the heater is also provided with a steam inlet and a condensed water outlet, and the condensed water outlet pipeline passes through a valve F20 and then is combined with a water inlet pipeline from a valve F19 to the top of the hot water recovery tank to enter the hot water recovery tank;

a branch water inlet pipeline is arranged on the pipeline between the valve F17 and the valve F18 and is connected with a valve F21;

a branch water inlet pipeline is arranged between the valve F20 and a water inlet pipeline at the top of the hot water recovery tank and is connected with a valve F22.

By adopting the technical scheme, the ginseng can be extracted by ethanol aqueous solutions with different concentrations in a differential gradient manner, so that the effective components in the ginseng, such as ginsenoside, polysaccharide, polypeptide and the like, can be more comprehensively extracted by the ethanol aqueous solutions with different concentrations, the concentration gradient of the ginseng and the solvent can be increased to the maximum extent by keeping the ethanol aqueous solution used for extraction in a new solvent state all the time, the extraction rates of active substances, such as ginsenoside, polysaccharide, polypeptide and the like, and the ethanol can be reused in a production system through a heater, a gas-liquid separator, a cooler and a condensate storage tank, so that the ethanol consumption is saved to the maximum extent, and the cost is reduced.

Furthermore, in the integrated extraction process of the ginseng, active substances are synchronously concentrated and separated to enter a fermentation tank for fermentation, so that the overall time of extraction, concentration and fermentation is greatly shortened, adverse factors such as foaming and the like caused by excessive accumulation of saponin during separate concentration are reduced, the active substances obtained in the extraction process can be transferred in time, the phenomena of decomposition, color change and the like are reduced, the concentration process is equivalent to sterilization, and the risk of microbial infection is reduced by conveying through a fully-closed pipeline.

In addition, the solvent steam that the heater produced gets into the lime set storage tank through the condenser, and a part is as extracting water fluid infusion after the cooling water heat transfer of this process, and a part is as fermentation solvent fluid infusion, and a part carries out secondary heat transfer (cooler) to being used for further cooling concentrate, gets into hot water recovery tank at last, also gets into the heating source that acts as fermentation process in the hot water recovery tank after the steam cooling that the heater used, and this part of water storage can regard as whole integrated system's washing water at last simultaneously. The integrated scheme integrates and fully utilizes water resources and energy, greatly saves water, reduces energy consumption and is more environment-friendly.

Further, it turns over the board distributor to draw jar interior upper portion to be equipped with, the kettle cover bottom downwardly extending who draws the jar has the connecting rod, the top of connecting rod is fixed with the kettle cover who draws the jar, and its bottom is articulated with the top surface that turns over the board distributor, the both sides that turn over the board distributor all are provided with the baffle, one the baffle is located the top that turns over the board distributor and fixed with drawing jar inner wall, another the baffle is located the bottom that turns over the board distributor and fixed with drawing jar inner wall, under the unproduction state, it is the horizontality to turn over the board distributor.

Through adopting above-mentioned scheme, when needs add solid material, keep turning over the board distributor and erect, when the mixed liquid of needs interpolation alcohol and water, keep turning over board distributor level, the circular foraminiferous structure on distributor surface can make alcohol and water ability misce bene and evenly distributed enter into and draw the jar in to the ginseng powder and draw.

In a second aspect, the present application provides a method for producing ginseng ferment, which adopts the following technical scheme:

a production method of ginseng ferment specifically comprises the following steps:

adding glucose and yeast powder in a fermentation medium in advance into a fermentation tank, inoculating 1% of lactobacillus plantarum seed liquid in volume percentage under an aseptic condition, and controlling the pH to be 5.5 and the temperature to be 37 ℃;

the fermentation medium comprises the following raw material components in parts by weight: 200 parts of concentrated solution, 20 parts of glucose and 1 part of yeast;

the concentrated solution and the ginseng ferment are obtained through the following steps:

(1) pulverizing Ginseng radix into 5-10 mesh Ginseng radix powder, adding the pulverized Ginseng radix powder into an extraction tank, and controlling the filling amount of Ginseng radix powder to 50-70% of the volume of the extraction tank;

(2) closing valve F5, valve F8, valve F10, opening valve F2, first transfer pump, valve F3, valve F15, valve F7, valve 11, valve F12, third transfer pump, by controlling the opening degree of the valve F3, the valve F12 and the valve F15, 95 to 100 percent of ethanol from the ethanol storage tank is mixed with water passing through the valve F15 to obtain an ethanol water solution with the volume percentage concentration of 80 percent, and adding into an extraction tank for extracting Ginseng radix powder in the extraction tank for the first time, wherein the volume percentage concentration of 80% ethanol water solution is 2-3 times of the weight of Ginseng radix powder per hour, when the total amount of the ethanol water solution with the volume percentage concentration of 80 percent is 2 to 4 times of the total amount of the ginseng powder, closing the valve F3, and stopping the first alcohol extraction of the ginseng powder by the ethanol water solution with the volume percentage concentration of 80%;

in the process of adding 80 percent by volume of ethanol water solution to carry out alcohol extraction on the ginseng powder, the temperature of an extraction tank is controlled to be 50-60 ℃;

in the process of extracting the ethanol aqueous solution with the volume percentage concentration of 80%, the circulating concentration of the extracting solution among the valve F11, the third delivery pump, the valve F9, the heater and the gas-liquid separator is realized by controlling the valve F11, the third delivery pump, the valve F9, the heater and the gas-liquid separator;

in the cyclic concentration process, micromolecular volatile substances extracted from the extracting solution and an ethanol water solution with the volume percentage concentration of 80% pass through a heater, are separated by a gas-liquid separator, and then enter a condensate storage tank through a condenser to obtain a recycled ethanol water solution;

in the cyclic concentration process, opening the valve F10 and the fifth delivery pump for 2 times per hour, opening for 1-2min each time, simultaneously closing the third delivery pump, concentrating the extracting solution into concentrated solution, cooling the concentrated solution in a cooler, and cooling the concentrated solution and then synchronously fermenting in a fermentation tank;

(3) after the valve F3 in the step (2) is closed, immediately opening the valve F4, the second delivery pump and the valve F5 to add the recycled reusable ethanol aqueous solution into the extraction tank, and realizing the second alcohol extraction of the ginseng powder by the ethanol aqueous solution by controlling the amount of the recycled reusable ethanol aqueous solution and the water entering the extraction tank through the valve F15;

in the second alcohol extraction process, the volume percentage concentration of the ethanol aqueous solution is controlled to be uniformly reduced from 80 percent to 0 percent within 2.5-3 hours, so as to realize differential gradient extraction;

controlling the temperature at 50-60 deg.C and the flow rate at 2-3 times of the weight of Ginseng radix powder/h during the second alcohol extraction;

in the differential gradient extraction process, the circulating concentration of the extracting solution among the valve F11, the third delivery pump, the valve F9, the heater and the gas-liquid separator is realized by controlling the valve F11, the third delivery pump, the valve F9, the heater and the gas-liquid separator;

in the cyclic concentration process, micromolecular volatile substances extracted from the extracting solution and ethanol water solutions with different volume percentage concentrations pass through a heater, are separated by a gas-liquid separator and then enter a condensate storage tank through a condenser to obtain a recycled ethanol water solution;

(4) when the volume percentage concentration of the ethanol water solution is reduced to 10%, opening a valve F1, and adding mixed enzyme into an extraction tank for enzymolysis at the temperature of 50-60 ℃; after enzymolysis for 1-1.5h, the volume percentage concentration of the ethanol water solution is reduced from 10% to 0%;

(5) closing the valve F10 and the fifth delivery pump, continuing to ferment for 42-43.5h to obtain a fermentation liquid, filtering, and adding butanediol with the same volume to obtain the ginseng ferment;

the filtering pore diameter of the pipeline filter is 100-200 nm;

the mixed enzyme comprises cellulase, hemicellulase and pectinase, wherein the addition amounts of the cellulase, hemicellulase and pectinase are 20IU/g, 20IU/g and 4IU/g respectively;

the activity of the cellulase is 100000-150000IU/g, the activity of the hemicellulase is 15000-20000IU/g, and the activity of the pectinase is 15000-20000 IU/g;

the seed liquid is obtained by the following preparation steps:

taking 50mL of liquid MRS culture medium, sterilizing, cooling, pouring food-grade lactobacillus plantarum frozen at a low temperature under the protection of a tube of glycerol into a horizontal laminar flow table, shaking up, culturing at a constant temperature of 37 ℃ for 24-48h, then using the culture solution to scratch a flat plate containing the solid MRS culture medium, picking a single colony from the flat plate, inoculating the single colony into a seed culture medium for seed culture, standing, controlling the temperature in the seed culture process to be 37 ℃, and culturing for 48h to obtain a seed solution;

the seed culture medium comprises the following raw material components in parts by weight: 200 parts of concentrated solution, 2 parts of glucose and 1 part of yeast powder.

By adopting the technical scheme, because the ethanol differential gradient extraction is adopted, the ethanol solvent is continuously concentrated and recovered in the extraction process, and the ethanol water solution with different concentrations can be used for carrying out the solvent differential gradient extraction on the ginseng only by continuously supplementing water. By adopting differential gradient extraction, effective active ingredients of ginseng, such as saponin, polysaccharide, polypeptide and the like, can be more comprehensively extracted by using ethanol aqueous solutions with different concentrations, the solvent can be always kept in a new solvent state, the concentration gradient of medicinal materials and the solvent is increased to the maximum extent, the extraction rate of active substances is improved, and ethanol is repeatedly used in a system, so that the ethanol consumption and the cost are saved to the maximum extent.

Furthermore, active matters in the ginseng extract are synchronously concentrated and separated in the extraction process and enter a fermentation tank for fermentation, so that the overall time of extraction, concentration and fermentation is greatly shortened, adverse factors such as air bubbles and the like caused by excessive accumulation of saponin during individual concentration are reduced, the phenomena of decomposition, color change and the like are reduced even if the active matters obtained in the extraction process are transferred, and meanwhile, the concentration process is equivalent to sterilization and is conveyed through a fully-closed pipeline, so that the risk of microbial infection is reduced.

In addition, the dregs of a decoction are subjected to enzymolysis in the extraction process, wherein the cellulase mainly has the function of decomposing cellulose in the ginseng powder into oligosaccharide and glucose; the hemicellulase has the main function of carrying out enzymolysis on the hemicellulose into oligomeric xylan, oligomeric mannan, xylose and mannose; the pectinase has the main function of hydrolyzing pectin into water-soluble pectin, pectic acid and beta-galacturonic acid, so that the utilization rate of effective components in the ginseng powder by a strain in the next fermentation process is improved, and the decomposed cellulose can also be used as a carbon source in the fermentation process, thereby saving the cost and reducing the solid waste rate.

Further, constant-temperature water in the hot water reflux tank enters a fermentation tank jacket through a valve F23 and a fourth delivery pump, and then flows back to the hot water reflux tank from the fermentation tank jacket, so that the fermentation temperature in the fermentation tank is controlled at 37 ℃;

the constant-temperature water in the hot water return tank enters a cooler through a valve F23, a fourth delivery pump, a valve F18 and a valve F17 to cool the concentrated solution, and then flows out of the cooler and returns to the hot water recovery tank through a valve F19;

the temperature of the water entering the cooler is controlled by controlling the valve F21 and the temperature of the water entering the hot water recovery tank is controlled by controlling the valve F22.

Through adopting above-mentioned scheme, adopt hot water recovery tank can realize the accuse temperature to the fermentation cylinder, can realize cooling the concentrate in the cooler again, and realized water cyclic utilization's function, make full use of water resource and energy, practice thrift water, reduce the energy consumption by a wide margin, be more friendly to the environment.

Further, the liquid MRS culture medium is composed of the following raw materials in parts by weight: 10 parts of peptone, 5 parts of beef extract powder, 4 parts of yeast powder, 20 parts of glucose, 2 parts of dipotassium hydrogen phosphate, 2 parts of triammonium citrate, 5 parts of sodium acetate, 0.2 part of magnesium sulfate, 0.05 part of manganese sulfate and 801 parts of tween; the pH value of the liquid MRS culture medium is 6.2 +/-0.2;

the raw material composition of the solid MRS culture medium is that 15 parts of agar is also contained on the basis of the formula of the liquid MRS culture medium.

By adopting the technical scheme, the liquid MRS culture medium is mainly used for activating microorganisms, and the solid culture medium is mainly used for culturing microorganisms and selecting optimal colonies by plate streaking.

In a third aspect, the present application provides a ginseng ferment obtained by the above production method.

Further, the ginsenoside comprises 1000-1500mg of 20(S) ginsenoside Rg3, 500-1000mg of 20(R) ginsenoside Rg3 and 200-400mg of ginsenoside Rh2 per liter.

Through adopting above-mentioned technical scheme, compare in the ginseng ferment of market, the ginseng ferment that this application provided, wherein rare saponin total amount has great improvement to the bioavailability also correspondingly improves greatly.

In a fourth aspect, the present application provides an application of the above ginseng ferment, which adopts the following technical scheme:

the application of the ginseng ferment is characterized in that the ginseng ferment is added into a cosmetic formula, and the cosmetic comprises water, milk, cream, essence and the like.

By adopting the technical scheme, the ginseng ferment obtained by the production method is added into the cosmetics, the skin is externally used for more than 2 weeks, the proliferation of fibroblasts can be promoted, the proliferation of scar cells is inhibited, the effects of accelerating skin barrier repair and improving skin smoothness are achieved, and the ginseng ferment can be used for skin care directions such as new scar repair, pockmark repair and skin color brightening.

Further, the ginseng ferment is added into the cosmetic or the ointment in an amount of 0.1-2%.

By adopting the technical scheme, the optimal addition amount of the ginseng ferment in the cosmetics or the ointment is 0.1-2%, and the obtained product has high effectiveness in promoting the proliferation of fibroblasts and inhibiting the proliferation of scar cells.

In summary, the present application has the following beneficial effects:

(1) according to the method, the active substances are synchronously concentrated and separated in the extraction process and enter the fermentation tank for fermentation through the integrated extraction, concentration and fermentation processes, so that the overall time is shortened, the phenomenon of adverse factors caused by excessive accumulated saponin is reduced, the concentration process is equivalent to sterilization, the materials are conveyed through a fully-closed pipeline, and the risk of microbial infection is reduced;

(2) according to the application, through the integration scheme, water resources and energy are comprehensively utilized, water is greatly saved, energy consumption is reduced, and the method is more environment-friendly;

(3) according to the method, the ethanol solvent is continuously concentrated and recovered, and the solvent differential gradient extraction of the ginseng by the solvents with different polarities can be realized as long as water is continuously supplemented, so that the maximum extraction of active substances in the ginseng is realized, the extraction rate of the active substances is improved, and the ethanol is repeatedly used in the system, so that the ethanol consumption is saved to the maximum extent, and the production cost is reduced;

(4) by combining the extraction and enzymolysis processes, the cellulose in the ginseng is decomposed to a certain degree and becomes a carbon source in the fermentation process, so that the treatment cost of the medicine residue is reduced, and the fermentation cost is saved;

(5) the components of the ginseng are fully extracted and enzymolyzed to form the concentrated solution for fermentation, so that the full components of the ginseng medicinal material are kept to participate in the fermentation to the greatest extent, the defects that the purely applicable ginseng medicinal material occupies the fermentation volume and the contact between microorganisms and the medicinal material is insufficient are avoided, and the defects that the fermentation is not complete because only some extracted components of the ginseng are used in the traditional method are also avoided;

(6) the ginseng ferment that this application production obtained, in the aspect of the restoration of skin external use and suppression scar, the effect has obviously to promote.

Drawings

FIG. 1 is a flowchart illustrating an integrated system for producing ginseng ferment according to an embodiment of the present disclosure;

fig. 2 is a schematic view of a distribution structure of a flap distributor in an extraction tank according to an embodiment of the present application.

Reference numerals: 1. heating and storing the ethanol; 2. a condensate storage tank; 3. an extraction tank; 4. a hot water recovery tank; 5. a fermentation tank; 6. a condenser; 7. a cooler; 8. an enzyme mixing tank; 9. a heater; 10. a gas-liquid separator; 11. an alcohol-water mixing tank; 12. a flap distributor; 13. a filter screen; 14. a pipeline filter; 15. a first delivery pump; 16. a second delivery pump; 17. A third delivery pump; 18. a fourth delivery pump; 19. a fifth delivery pump; 20. and a baffle plate.

Detailed Description

The present application will be described in further detail with reference to the following drawings and examples.

Examples

Example one

An integrated system for producing ginseng ferment is shown in fig. 1, and comprises an ethanol heating storage tank 1, a condensate storage tank 2, an extraction tank 3, a hot water recovery tank 4, a fermentation tank 5, a condenser 6, a cooler 7, an enzyme mixing tank 8, a heater 9, a gas-liquid separator 10 and an alcohol-water mixing tank 11.

The top of extraction tank 3 is provided with enzyme mixed liquor inlet pipeline, alcohol-water mixed liquor inlet pipeline, and its lateral wall is equipped with extract reflux inlet pipeline, and the bottom is equipped with the extract export.

Draw jar 3 interior upper portion to be equipped with and turn over board distributor 12, it is circular even foraminiferous structure, the kettle cover bottom downwardly extending who draws jar 3 has the connecting rod, the top of connecting rod is fixed with the kettle cover who draws jar 3, its bottom is freely articulated with the top surface that turns over board distributor 12, the both sides that turn over board distributor 12 all are provided with baffle 20, a baffle 20 is located the top that turns over board distributor 12 and with draw jar 3 inner wall fixed, another baffle 20 is located the bottom that turns over board distributor 12 and with draw jar 3 inner wall fixed. When solid materials are added, the flap distributor 12 is erected under the action of the gravity of the materials, and the materials fall down; when two liquid materials are added, the turning plate distributor 12 is kept horizontal, and the two liquids on the turning plate distributor are uniformly mixed and seep down to ensure that the two liquid materials are uniformly mixed. The lower part in the extraction tank 3 is provided with a filter screen 13 for primarily intercepting the ginseng powder residue after alcohol extraction, the aperture of the filter screen 13 can be 40-60 meshes, in the embodiment, the aperture of the filter screen 13 is set to be 50 meshes.

The outlet at the bottom of the enzyme mixing tank 8 is connected with an enzyme mixed liquid inlet pipeline arranged at the top of the extraction tank 3 through a valve F1 by a pipeline.

The top of the alcohol-water mixing tank 11 is provided with an alcohol inlet pipeline and a water inlet pipeline, and the bottom of the alcohol-water mixing tank is provided with an alcohol-water mixed liquid outlet.

The alcohol-water mixed liquid outlet at the bottom of the alcohol-water mixing tank 11 is connected with the alcohol-water mixed liquid inlet pipeline arranged at the top of the extraction tank 3 through a pipeline.

The bottom outlet of the ethanol heating storage tank 1 is connected with an ethanol inlet pipeline at the top of the ethanol-water mixing tank 11 through a valve F2, a first delivery pump 15 and a valve F3, and the volume percentage concentration of the ethanol solution in the ethanol heating storage tank 1 is 95-100%.

The bottom outlet of the condensate storage tank 2 is connected with an ethanol inlet pipeline at the top of the alcohol-water mixing tank 11 through a valve F4, a second delivery pump 16 and a valve F5; the pipeline where the second delivery pump 16 is located is provided with a pipeline bypass, the pipeline bypass is provided with a valve F6, the valve F6 is located between the valve F4 and the valve F5, and when the second delivery pump 16 works, the valve F6 is closed or partially opened to adjust the flow rate, so that the flow rate is more stable.

The bottom of the extraction tank 3 is divided into two paths through a valve F7, a third delivery pump 17 and a pipeline filter 14, the first path is connected with a reflux inlet pipeline on the side wall of the extraction tank 3 after passing through a valve F8, and the first path is used for cleaning the extraction tank 3 and pipelines; the second way links to each other with the inlet pipe that heater 9 bottom was equipped with behind valve F9, and the export pipeline tangent line at heater 9 top enters vapour and liquid separator 10, and the top export of vapour and liquid separator 10 links to each other through the inlet pipe that pipeline and condenser 6 top were equipped with, and the condensate export of condenser 6 bottom links to each other with the inlet pipe that condensate storage tank 2 top was equipped with, and this route mainly is used for separating out ethanol from the extract and drops into in condensate storage tank 2 and carry out cyclic utilization.

The top of the fermentation tank 5 is provided with a water inlet pipeline which is communicated with a valve F14.

Extract ginseng extract that the extract export of extracting tank 3 bottom came out after reaching concentration through valve F10 and fifth delivery pump 19 after getting into 7 material pipeline entries of cooler, the material outlet pipeline of cooler 7 and the water inlet pipeline that 5 tops of fermentation cylinder were equipped with merge and get into fermentation cylinder 5, this route can be guaranteed to extract the in-process, has partial extract to ferment in entering into fermentation cylinder 5 after concentrating simultaneously, shortens ginseng ferment's whole production time.

The filtering pore diameter of the pipe filter 14 may be 100-200nm, and in this embodiment, the pore diameter of the pipe filter 14 is preferably 100 meshes.

The bottom outlet pipe of the gas-liquid separator 10 is merged with the pipe between the bottom outlet valve F7 of the extraction tank 3 and the third transfer pump 17 after passing through the valve F11.

The condenser 6 is also provided with a cooling water inlet and a cooling water outlet, and the cooling water outlet is merged with a water inlet pipeline at the top of the fermentation tank 5 and a pipeline between the valves F14 and enters the fermentation tank 5 after passing through a valve F12 and a valve F13 through pipelines; the water inlet pipeline of the fermentation tank 5 is connected with a valve F14, which can further supplement water for fermentation.

A branch pipeline is arranged between a cooling water outlet of the condenser 6 and a valve F12, the branch pipeline is connected with a water inlet pipeline at the top of the alcohol-water mixing tank 11 after passing through a valve F15, cooling water enters the alcohol-water mixing tank 11 to be mixed with ethanol, ethanol water solutions with different volume percentage concentrations can be formed, an extraction solvent is provided for ginseng powder in the extraction tank 3, wherein the valve F3 and the valve F5 are switched to feed, so that the ethanol water solutions with different concentrations perform differential gradient extraction on ginseng, and the extraction is more comprehensive and more sufficient.

A branch pipeline is arranged on the pipeline between the valve F12 and the valve F13 and is connected with a cooling water inlet pipeline arranged at the bottom of the cooler 7 through the valve F16, and a cooling water outlet arranged at the top of the cooler 7 is connected with a water inlet pipeline arranged at the top of the hot water recovery tank 4 through the valve F19.

The bottom outlet of the hot water recovery tank 4 is divided into two paths after passing through a valve F23 and a fourth delivery pump 18, the first path enters a jacket of the fermentation tank 5 through a valve F24, the top of the jacket of the fermentation tank is provided with an outlet and is connected with a return pipe inlet arranged at the top of the hot water recovery tank 4 through a pipeline so as to control the fermentation temperature of the fermentation tank 5; the second path is merged into the cooler 7 through a valve F18, a valve F17 and a cooling water inlet pipeline of the cooler 7.

The heater 9 is also provided with a steam inlet and a condensed water outlet, and the condensed water outlet pipeline passes through a valve F20 and then is merged with a water inlet pipeline from the valve F19 to the top of the hot water recovery tank 4 to enter the hot water recovery tank 4.

A branch inlet line is provided in the line between valve F17 and valve F18 and is connected to valve F21 for replenishing the cooler 7 with water.

A branch water inlet pipeline is arranged between the valve F20 and the water inlet pipeline at the top of the hot water recovery tank 4 and is connected with a valve F22 for supplementing water to the hot water recovery tank 4.

The valve F3, the valve F5 and the valve F15 in the embodiment are all automatic valves, and other valves are all manual valves.

Example 1

Referring to fig. 1, a method for producing ginseng ferment specifically comprises the following production steps:

in the fermentation tank 5, glucose in the fermentation medium is added in advance as a carbon source and yeast powder as a nitrogen source, and lactobacillus plantarum seed liquid with the volume percentage of 1% is inoculated under the aseptic condition, the pH is controlled to be 5.5, and the temperature is controlled to be 37 ℃.

The fermentation medium comprises the following raw material components in parts by weight: 200 parts of concentrated solution, 20 parts of glucose and 1 part of yeast.

The concentrated solution and the human ferment are obtained by the process comprising the following steps:

(1) pulverizing Ginseng radix into 5 mesh, and putting the pulverized Ginseng radix powder into extraction tank 3, wherein the volume of the medicinal materials accounts for 60% of that of the extraction tank 3.

(2) Valve F5, valve F8, valve F10 are closed, valve F2, first feed pump 15, valve F3, valve F15, valve F7, valve 11, valve F12, third feed pump 17 are opened, by controlling the opening degree of the valve F3, the valve F12 and the valve F15, 95 to 100 percent of ethanol from the ethanol storage tank is mixed with water passing through the valve F15 to obtain an ethanol water solution with the volume percentage concentration of 80 percent, and adding into the extraction tank 3 for extracting Ginseng radix powder in the extraction tank 3 for the first time, maintaining the temperature at 60 deg.C, adding 80% ethanol water solution at a volume percentage concentration of 2 times the weight/h of Ginseng radix powder, when the total amount of the ethanol water solution with the volume percentage concentration of 80 percent is 4 times of the total amount of the ginseng powder, the valve F3 is closed, and the first alcohol extraction of the ginseng powder with 80% by volume aqueous ethanol is stopped.

(3) After the valve F3 in the step (2) is closed, immediately opening and controlling the valve F4, the second delivery pump 16 and the valve F15, adding the recycled reusable ethanol water solution into the ethanol-water mixing tank 11, realizing the second ethanol extraction of the ginseng powder by the ethanol water solution by controlling the amount of the recycled reusable ethanol water solution and the water entering the extraction tank 3 through the valve F15, keeping the temperature and the speed unchanged, and gradually reducing the volume percentage concentration of the ethanol water solution from 80% to 0% within 2.5 h;

in the alcohol extraction of the 80% ethanol aqueous solution in the step (2) and the differential gradient extraction in the step (3), the circulating concentration of the extracting solution among the valve F11, the third delivery pump 17, the valve F9, the heater 9 and the gas-liquid separator 10 is realized by controlling the valve F11, the third delivery pump 17, the valve F9, the heater 9 and the gas-liquid separator 10. In the circulating concentration process, micromolecular volatile substances extracted from the extracting solution and ethanol water solution with different volume percentage concentrations pass through a heater 9, are separated through a gas-liquid separator 10, pass through a condenser 6 and enter a condensate storage tank 2, and the recovered and reusable ethanol water solution is obtained. In the whole circulation concentration process, the valve F10 and the fifth delivery pump are opened 192 times per hour, the valves are opened for 1.5min each time, and the third delivery pump 17 is closed at the same time, so that the extracting solution is concentrated into concentrated solution and enters the cooler 7 for cooling, and then enters the fermentation tank 5 for synchronous fermentation after cooling.

(4) When the volume percentage concentration of the ethanol water solution is reduced to below 10%, a valve F1 is opened, mixed enzyme consisting of cellulase, hemicellulase and pectinase is added into the extraction tank 3 for enzymolysis, the activities are 150000IU/g, 20000IU/g and 20000IU/g respectively, the total weight of the ginseng powder is calculated, the adding amounts are 20IU/g, 20IU/g and 4IU/g respectively, the enzymolysis time is 1h, and the temperature and the speed of the ethanol water solution are kept unchanged.

(5) And (3) closing the valve F10 and the fifth delivery pump 19, continuing to ferment for 43.5 hours to obtain a fermentation liquid, filtering, and adding butanediol with the same volume to obtain the ginseng ferment.

The lactobacillus plantarum seed solution is prepared by the following preparation steps:

taking 50mL of liquid MRS culture medium, sterilizing, cooling, pouring a tube of food-grade lactobacillus plantarum frozen at a low temperature under the protection of glycerol into a horizontal laminar flow table, shaking uniformly, wherein the food-grade lactobacillus plantarum is purchased from Shanghai preservation Biotechnology center (SHBCC) (strain number: SHBCC D18303), culturing at a constant temperature of 37 ℃ for 30h, then using the culture solution to plate the plate containing the MRS culture medium, selecting a single colony from the plate, inoculating the single colony into a seed culture medium for seed culture, standing, controlling the temperature in the seed culture process to be 37 ℃, and culturing for 48h to obtain the lactobacillus plantarum seed solution.

The liquid MRS culture medium comprises the following raw materials in parts by weight: 10 parts of peptone, 5 parts of beef extract powder, 4 parts of yeast powder, 20 parts of glucose, 2 parts of dipotassium hydrogen phosphate, 2 parts of triammonium citrate, 5 parts of sodium acetate, 0.2 part of magnesium sulfate, 0.05 part of manganese sulfate and 801 parts of tween; the pH value of the liquid MRS culture medium is 6.2 plus or minus 0.2.

Wherein the raw material composition of the solid MRS culture medium is that the liquid MRS culture medium also contains 15 parts of agar on the basis of the formula of the liquid MRS culture medium.

Example 2

The method for producing the ginseng ferment is different from the embodiment 2 in that the method specifically comprises the following production steps:

The concentrated solution and the ginseng ferment are obtained by a process comprising the steps of:

(1) pulverizing Ginseng radix into 10 mesh, and putting the pulverized Ginseng radix powder into extraction tank 3, wherein the volume of the medicinal materials accounts for 70% of that of the extraction tank 3;

(2) valve F5, valve F8, valve F10 are closed, valve F2, first feed pump 15, valve F3, valve F15, valve F7, valve 11, valve F12, third feed pump 17 are opened, by controlling the opening degree of the valve F3, the valve F12 and the valve F15, 95 to 100 percent of ethanol from the ethanol storage tank is mixed with water passing through the valve F15 to obtain an ethanol water solution with the volume percentage concentration of 80 percent, and adding into the extraction tank 3 for extracting Ginseng radix powder in the extraction tank 3 for the first time, maintaining the temperature at 50 deg.C, adding 80% ethanol water solution at a volume percentage concentration 3 times the weight/h of Ginseng radix powder, when the total amount of the ethanol water solution with the volume percentage concentration of 80 percent is 2 times of the total amount of the ginseng powder, the valve F3 is closed, and the first alcohol extraction of the ginseng powder with 80% by volume aqueous ethanol is stopped.

(3) Immediately opening and controlling the valve F4, the second delivery pump 16 and the valve F15 after the valve F3 in the step (2) is closed, adding the recycled reusable ethanol water solution into the ethanol-water mixing tank 11, realizing the second ethanol extraction of the ginseng powder by the ethanol water solution by controlling the amount of the recycled reusable ethanol water solution and the water entering the extraction tank 3 through the valve F15, keeping the temperature and the speed unchanged, and gradually reducing the volume percentage concentration of the ethanol water solution from 80 percent to 0 percent within 3 hours.

(4) When the volume percentage concentration of the ethanol water solution is reduced to below 10%, opening a valve F1, adding mixed enzyme consisting of cellulase, hemicellulase and pectinase into an extraction tank 3 for enzymolysis, wherein the activities are 100000IU/g, 15000IU/g and 15000IU/g respectively, the adding amounts are 20IU/g, 20IU/g and 4IU/g respectively calculated by the total weight of the ginseng powder, the enzymolysis time is 1h, and the temperature and the speed of the ethanol water solution are kept unchanged;

The procedure for the preparation of the lactobacillus plantarum seed liquid was the same as that of example 2.

Example 3

(1) pulverizing Ginseng radix into 10 mesh, and putting the pulverized Ginseng radix powder into extraction tank 3, wherein the volume of the medicinal materials accounts for 50% of that of the extraction tank 3;

(2) valve F5, valve F8, valve F10 are closed, valve F2, first feed pump 15, valve F3, valve F15, valve F7, valve 11, valve F12, third feed pump 17 are opened, by controlling the opening degree of the valve F3, the valve F12 and the valve F15, 95 to 100 percent of ethanol from the ethanol storage tank is mixed with water passing through the valve F15 to obtain an ethanol water solution with the volume percentage concentration of 80 percent, and adding into the extraction tank 3 for extracting Ginseng radix powder in the extraction tank 3 for the first time, maintaining the temperature at 60 deg.C, adding 80% ethanol water solution at a volume percentage concentration of 2.5 times the weight/h of Ginseng radix powder, when the total amount of the ethanol water solution with the volume percentage concentration of 80 percent is 3 times of the total amount of the ginseng powder, the valve F3 is closed, and the first alcohol extraction of the ginseng powder with 80% by volume aqueous ethanol is stopped.

(3) Immediately opening and controlling the valve F4, the second delivery pump 16 and the valve F15 after the valve F3 in the step (2) is closed, adding the recycled reusable ethanol water solution into the ethanol-water mixing tank 11, realizing the second ethanol extraction of the ginseng powder by the ethanol water solution by controlling the amount of the recycled reusable ethanol water solution and the water entering the extraction tank 3 through the valve F15, keeping the temperature and the speed unchanged, and gradually reducing the volume percentage concentration of the ethanol water solution from 80% to 0% within 2.5 h.

(4) When the volume percentage concentration of the ethanol water solution is reduced to below 10%, opening a valve F1, adding mixed enzyme consisting of cellulase, hemicellulase and pectinase into the extraction tank 3 for enzymolysis, wherein the activities are 150000IU/g, 20000IU/g and 20000IU/g respectively, the total weight of the ginseng powder is calculated, the adding amounts are 20IU/g, 20IU/g and 4IU/g respectively, and the temperature and the speed of the ethanol water solution are kept unchanged;

Application example

Application example 1

A ginseng ferment cream comprises 2% of ginseng ferment prepared in the embodiment 2, and further comprises the following components in percentage by mass: deionized water 63.5%, glycerol 6%, butanediol 6%, allantoin 0.2%, sodium hyaluronate 0.1%, methylparaben 0.2%, cetearyl alcohol 1%, steareth-21%, steareth-212%, shea butter 3%, caprylic/capric triglyceride 7%, squalane 4%, tocopheryl acetate 0.50.9%, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer 1%, panthenol 2%, essence 0.15%, 1, 2-hexanediol 0.6%.

The essence in the application example is purchased from ZC2930, Guangzhou Huawen essence and spice Co.

Application example 2

A ginseng ferment emulsion comprises 2% of ginseng ferment prepared in the embodiment 2, and further comprises the following components in percentage by mass: deionized water 70.5%, glycerol 5%, butanediol 5%, allantoin 0.2%, sodium hyaluronate 0.1%, methylparaben 0.15%, cetearyl alcohol 0.6%, steareth-20.7%, steareth-211.5%, jojoba oil 3%, caprylic/capric triglyceride 6%, squalane 3%, tocopherol acetate 0.5%, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer 0.5%, panthenol 1%, essence 0.1%, 1, 2-hexanediol 0.6%.

Application example 3

A ginseng ferment essence comprises 1 wt% of the ginseng ferment prepared in the embodiment 2, and further comprises the following components in percentage by mass: 89.75% of deionized water, 0.25% of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, 5% of glycerol, 0.1% of xanthan gum, 0.15% of sodium hyaluronate, 0.15% of methylparaben, 0.25% of allantoin, 0.15% of chlorphenesin, 0.5% of 10% sodium hydroxide aqueous solution, 0.2% of centella asiatica extract, 0.5% of panthenol and 2% of plant extraction repair factor.

The centella asiatica extract and the plant extraction and repair factor in the application example are both obtained from Guangzhou Galen biotechnology limited, wherein the plant extraction and repair factor comprises water, chlorella extract, sophora bud extract and linseed extract.

Application example 4

A ginseng ferment water comprises 0.5% of ginseng ferment prepared in the embodiment 2, and further comprises the following components in percentage by mass: 92.28% of deionized water, 5% of glycerol, 0.02% of sodium hyaluronate, 0.15% of methylparaben, 0.1% of allantoin, 0.3% of panthenol, 0.05% of tetrahydro-methyl pyrimidine carboxylic acid, 1% of plant extraction repair factor and 0.6% of 1, 2-hexanediol.

The plant extraction repair factor in the application example is purchased from Guangzhou Galen biotechnology limited, wherein the plant extraction repair factor comprises water, chlorella extract, sophora bud extract and linseed extract.

Comparative example

Comparative example 1

in a fermentation tank 5, glucose in a fermentation medium is added in advance as a carbon source and yeast powder as a nitrogen source, and lactobacillus plantarum seed liquid with the volume percentage of 1% is inoculated under the aseptic condition, the pH is controlled to be 5.5, and the temperature is controlled to be 37 ℃;

The concentrated solution and the ginseng ferment are obtained by the process comprising the following steps:

(1) pulverizing Ginseng radix into 5 mesh powder, and putting the pulverized Ginseng radix powder into extraction tank 3;

(2) maintaining the temperature at 60 deg.C, and extracting Ginseng radix powder with ethanol water solution 10 times the volume of the medicinal materials and 50% volume percentage concentration under reflux for 2 hr; carrying out reflux extraction on the ginseng powder for 2 hours by adopting ethanol water with 6 times volume amount and 50% volume percentage concentration of the medicinal material;

(3) cooling, filtering, concentrating, fermenting the concentrated solution with Lactobacillus plantarum in a fermentation tank 5 at 37 deg.C for 48 hr to obtain fermentation liquid, and adding equal volume of butanediol to obtain Ginseng radix ferment.

Comparative example 2

(1) pulverizing Ginseng radix into 5 mesh powder, and putting the pulverized Ginseng radix powder into extraction tank 3, wherein the volume of the medicinal materials accounts for 60% of that of the extraction tank 3;

(2) sequentially adding 2 times of 80% ethanol aqueous solution, 2 times of 70% ethanol aqueous solution, 2 times of 60% ethanol aqueous solution, 2 times of 50% ethanol aqueous solution, 2 times of 60% ethanol aqueous solution, 2 times of 70% ethanol aqueous solution, 2 times of 20% ethanol aqueous solution, 2 times of 10% ethanol aqueous solution and 2 times of water to carry out percolation extraction, wherein the temperature is maintained at 60 ℃, and the speed is 2 times of the weight of the medicinal materials per hour;

(3) filtering, combining filtrates, concentrating, cooling the concentrated solution, fermenting with Lactobacillus plantarum in a fermentation tank 5 at 37 deg.C for 48 hr to obtain fermentation liquid, filtering, and adding equal volume of butanediol to obtain Ginseng radix ferment.

Comparative example 3

pulverizing Ginseng radix into 5 mesh, irradiating with Co60 at a dose of 8kgy, adding into fermentation tank 5 at an amount of 10% of fermentation broth, adding 0.2% glucose as carbon source and 1% yeast extract powder as nitrogen source, inoculating 1% pre-cultured dominant Lactobacillus plantarum, and culturing at pH 5.5 and 37 deg.C for 48 h. Filtering, concentrating to 6 times of concentration, and adding butanediol with the same volume to obtain the ginseng ferment.

Comparative example 4

(1) pulverizing Ginseng radix into 12 mesh, and putting the pulverized Ginseng radix powder into extraction tank 3, wherein the volume of the medicinal materials accounts for 45% of that of the extraction tank 3;

(2) valve F5, valve F8, valve F10 are closed, valve F2, first feed pump 15, valve F3, valve F15, valve F7, valve 11, valve F12, third feed pump 17 are opened, by controlling the opening degree of the valve F3, the valve F12 and the valve F15, 95 to 100 percent of ethanol from the ethanol storage tank is mixed with water passing through the valve F15 to obtain an ethanol water solution with the volume percentage concentration of 85 percent, and adding into the extraction tank 3 for extracting Ginseng radix powder in the extraction tank 3 for the first time, maintaining the temperature at 65 deg.C, adding 85% ethanol water solution at a volume percentage concentration of 1.5 times the weight/h of Ginseng radix powder, when the total amount of the ethanol water solution with the volume percentage concentration of 85 percent is 5 times of the total amount of the ginseng powder, the valve F3 is closed, and the first alcohol extraction of the ginseng powder with 85% by volume aqueous ethanol is stopped.

(3) Immediately opening and controlling the valve F4, the second delivery pump 16 and the valve F15 after the valve F3 in the step (2) is closed, adding the recycled reusable ethanol water solution into the ethanol-water mixing tank 11, realizing the second ethanol extraction of the ginseng powder by the ethanol water solution by controlling the amount of the recycled reusable ethanol water solution and the water entering the extraction tank 3 through the valve F15, keeping the temperature and the speed unchanged, and gradually reducing the volume percentage concentration of the ethanol water solution from 85 percent to 0 percent within 4 hours.

In the alcohol extraction of the 85% ethanol aqueous solution in the step (2) and the differential gradient extraction in the step (3), the circulating concentration of the extracting solution among the valve F11, the third delivery pump 17, the valve F9, the heater 9 and the gas-liquid separator 10 is realized by controlling the valve F11, the third delivery pump 17, the valve F9, the heater 9 and the gas-liquid separator 10. In the circulating concentration process, micromolecular volatile substances extracted from the extracting solution and ethanol water solution with different volume percentage concentrations pass through a heater 9, are separated through a gas-liquid separator 10, pass through a condenser 6 and enter a condensate storage tank 2, and the recovered and reusable ethanol water solution is obtained. In the whole circulation concentration process, the valve F10 and the fifth delivery pump are opened 192 times per hour, the valves are opened for 1.5min each time, and the third delivery pump 17 is closed at the same time, so that the extracting solution is concentrated into concentrated solution and enters the cooler 7 for cooling, and then enters the fermentation tank 5 for synchronous fermentation after cooling.

(4) When the volume percentage concentration of the ethanol water solution is reduced to below 15%, opening a valve F1, adding mixed enzyme consisting of cellulase, hemicellulase and pectinase into an extraction tank 3 for enzymolysis, wherein the activities are 150000IU/g, 20000IU/g and 20000IU/g respectively, the total weight of the ginseng powder is calculated, the adding amounts are 20IU/g, 20IU/g and 4IU/g respectively, the enzymolysis time is 1h, and the temperature and the speed of the ethanol water solution are kept unchanged;

(5) and (3) closing the valve F10 and the fifth delivery pump 19, continuing to ferment for 42 hours to obtain a fermentation liquid, filtering, and adding butanediol with the same volume to obtain the ginseng ferment.

Comparative example 5

(1) pulverizing Ginseng radix into 4 mesh, and putting the pulverized Ginseng radix powder into extraction tank 3, wherein the volume of the medicinal materials accounts for 75% of that of the extraction tank 3;

(2) valve F5, valve F8, valve F10 are closed, valve F2, first feed pump 15, valve F3, valve F15, valve F7, valve 11, valve F12, third feed pump 17 are opened, by controlling the opening degree of the valve F3, the valve F12 and the valve F15, 95 to 100 percent of ethanol from the ethanol storage tank is mixed with water passing through the valve F15 to obtain an ethanol water solution with the volume percentage concentration of 75 percent, and adding into the extraction tank 3 for extracting Ginseng radix powder in the extraction tank 3 for the first time, maintaining the temperature at 65 deg.C, adding 75% ethanol water solution at a volume percentage concentration of 3.5 times the weight/h of Ginseng radix powder, when the total amount of the ethanol water solution with the volume percentage concentration of 75 percent is 1.5 times of the total amount of the ginseng powder, the valve F3 is closed, and the first alcohol extraction of the ginseng powder with 85% by volume aqueous ethanol is stopped.

(3) Immediately opening and controlling the valve F4, the second delivery pump 16 and the valve F15 after the valve F3 in the step (2) is closed, adding the recycled reusable ethanol water solution into the ethanol-water mixing tank 11, realizing the second ethanol extraction of the ginseng powder by the ethanol water solution by controlling the amount of the recycled reusable ethanol water solution and the water entering the extraction tank 3 through the valve F15, keeping the temperature and the speed unchanged, and gradually reducing the volume percentage concentration of the ethanol water solution from 75 percent to 0 percent within 2 hours.

In the alcohol extraction of the 75% ethanol aqueous solution in the step (2) and the differential gradient extraction in the step (3), the circulating concentration of the extracting solution among the valve F11, the third delivery pump 17, the valve F9, the heater 9 and the gas-liquid separator 10 is realized by controlling the valve F11, the third delivery pump 17, the valve F9, the heater 9 and the gas-liquid separator 10. In the circulating concentration process, micromolecular volatile substances extracted from the extracting solution and ethanol water solution with different volume percentage concentrations pass through a heater 9, are separated through a gas-liquid separator 10, pass through a condenser 6 and enter a condensate storage tank 2, and the recovered and reusable ethanol water solution is obtained. In the whole circulation concentration process, the valve F10 and the fifth delivery pump are opened 192 times per hour, the valves are opened for 1.5min each time, and the third delivery pump 17 is closed at the same time, so that the extracting solution is concentrated into concentrated solution and enters the cooler 7 for cooling, and then enters the fermentation tank 5 for synchronous fermentation after cooling.

(4) When the volume percentage concentration of the ethanol water solution is reduced to below 5%, opening a valve F1, adding mixed enzyme consisting of cellulase, hemicellulase and pectinase into an extraction tank 3 for enzymolysis, wherein the activities are 150000IU/g, 20000IU/g and 20000IU/g respectively, the total weight of the ginseng powder is calculated, the adding amounts are 20IU/g, 20IU/g and 4IU/g respectively, the enzymolysis time is 1h, and the temperature and the speed of the ethanol water solution are kept unchanged;

(5) and (3) closing the valve F10 and the fifth delivery pump 19, continuing to ferment for 46 hours to obtain a fermentation liquid, filtering, and adding butanediol with the same volume to obtain the ginseng ferment.

Comparative example 6

(1) pulverizing Ginseng radix into 5 mesh powder, and putting the pulverized Ginseng radix powder into extraction tank 3, wherein the volume of the medicinal materials accounts for 75% of that of the extraction tank 3;

(2) valve F5, valve F8, valve F10 are closed, valve F2, first feed pump 15, valve F3, valve F15, valve F7, valve 11, valve F12, third feed pump 17 are opened, by controlling the opening degree of the valve F3, the valve F12 and the valve F15, 95 to 100 percent of ethanol from the ethanol storage tank is mixed with water passing through the valve F15 to obtain an ethanol water solution with the volume percentage concentration of 80 percent, and adding into the extraction tank 3 for extracting Ginseng radix powder in the extraction tank 3 for the first time, maintaining the temperature at 65 deg.C, adding 80% ethanol water solution at a volume percentage concentration of 2 times the weight/h of Ginseng radix powder, when the total amount of the ethanol water solution with the volume percentage concentration of 80 percent is 4 times of the total amount of the ginseng powder, the valve F3 is closed, and the first alcohol extraction of the ginseng powder with 80% by volume aqueous ethanol is stopped.

(3) Immediately opening and controlling the valve F4, the second delivery pump 16 and the valve F15 after the valve F3 in the step (2) is closed, adding the recycled reusable ethanol water solution into the ethanol-water mixing tank 11, realizing the second ethanol extraction of the ginseng powder by the ethanol water solution by controlling the amount of the recycled reusable ethanol water solution and the water entering the extraction tank 3 through the valve F15, keeping the temperature and the speed unchanged, gradually reducing the volume percentage concentration of the ethanol water solution from 80% to 0% within 2.5h, and continuously extracting for 1 h.

(4) And (3) closing the valve F10 and the fifth delivery pump 19, continuing to ferment for 43.5 hours to obtain a fermentation liquid, filtering, and adding butanediol with the same volume to obtain the ginseng ferment.

Comparative example 7

(4) when the volume percentage concentration of the ethanol water solution is reduced to below 10%, a valve F1 is opened, mixed enzyme consisting of cellulase, hemicellulase and pectinase is added into the extraction tank 3 for enzymolysis, the activities are 150000IU/g, 20000IU/g and 20000IU/g respectively, the total weight of the ginseng powder is calculated, the adding amount is 20IU/g, 20IU/g and 4IU/g respectively, the enzymolysis time is 1h, the temperature and the speed of the ethanol water solution are kept unchanged, enzymatic hydrolysate is obtained, and after filtration, isometric butanediol is added, so that the ginseng ferment is obtained.

Performance test

The ginseng ferments prepared in examples 1 to 3 and comparative examples 1 to 7 were measured, and the results are shown in table 1 below.

The specific detection method and the calculation formula are as follows:

1. energy consumption is electricity consumption and medicinal material quality

The electricity consumption refers to the total electricity consumption in KJ of the whole process; the medicinal material quality refers to the feeding amount of the ginseng medicinal material in unit kg in the whole process.

2. Water consumption is the total water consumption divided by the mass of the medicinal materials

The total water consumption refers to the total water consumption in the whole process, and comprises process water, steam water and cooling water in kg; the medicinal material quality refers to the feeding amount of the ginseng medicinal material in unit kg in the whole process.

3. The total amount of ethanol is divided by the mass of the medicinal materials

The total amount of ethanol used refers to the total mass of ethanol in unit kg in the whole process; the medicinal material quality refers to the feeding amount of the ginseng medicinal material in unit kg in the whole process.

4. (M) extraction yield₀-M₁)/M₀*100％

M₀Grinding Ginseng radix into 120 mesh, weighing 2g, and testing water content₀Another m is taken₀Ultrasonically extracting the medicinal materials in 30 times of 50% ethanol for 30min, repeating for three times, mixing extractive solutions, concentrating, removing ethanol, ultrasonically extracting the residue with 30 times of water for 30min, repeating for three times, mixing all extractive solutions and concentrated solutions, and detecting to obtain total saponin content M₀₁Total sugar content M₀₂Total protein (polypeptide) content M₀₃，

M₀＝(M₀₁+M₀₂+M₀₃)/(m₀*H₀)*100％

M₁Drying the residue after extraction of Ginseng radix to water content less than 12%, pulverizing to 120 mesh, weighing 2g, and testing water content H₁Another m is taken₁Ultrasonically extracting the medicinal materials in 30 times of 50% ethanol for 30min, repeating for three times, mixing extractive solutions, concentrating, removing ethanol, ultrasonically extracting the residue with 30 times of water for 30min, repeating for three times, mixing all extractive solutions and concentrated solutions, and detecting to obtain total saponin content M₁₁Total sugar content M₁₂Total protein (polypeptide) content M₁₃。

M₁＝(M₁₁+M₁₂+M₁₃)/(m₁*H₁)*100％

Wherein the content of total saponins is detected according to vanillin-perchloric acid method;

detecting the total protein content according to the instruction of the kit for determining the protein content by Lowry method of Shanghai Lida Biotechnology Limited company, wherein the model of the kit is PRL 000100;

the total sugar content was determined according to the phenol-sulfuric acid method.

5. The solid waste rate is M dry weight of medicine residue ÷ M dry weight of medicinal materials ÷ 100%

M dry weight of the residue ═ mass of residue (1-water content of residue)

Dry weight of M herbs (mass of herbs) (1-water content of herbs).

6. Rare saponin content ═ M_{20(S) ginsenoside Rg3}+M_{20(R) ginsenoside Rg3}+M_{Ginsenoside Rh2})÷2M_{Fermentation liquor}

2M_{Fermentation liquor}The quality of the fermentation liquor and butanediol after equal proportion mixing and filtering.

Wherein the content of rare saponin is determined by HPLC method, which comprises:

preparing a test solution: sucking 5mL of sample solution to be detected, adding into a 10mL measuring flask, adding methanol to dilute to scale, shaking up, filtering, and taking the subsequent filtrate.

The determination method comprises the following steps: precisely sucking 10L of the test solution, injecting into a liquid chromatograph, measuring, and recording chromatogram. Calculating the contents of 20(S) ginsenoside Rg3, 20(R) ginsenoside Rg3 and ginsenoside Rh2 by standard curve method.

7. Fibroblast proliferation/inhibition

And (3) cell culture:

human Skin Fibroblast (HSF) or human scar fibroblast (HSFb) is prepared from high-sugar DMEM medium containing 100mL/L Fetal Bovine Serum (FBS), 100U/mL penicillin and 100U/mL streptomycin at 37 deg.C with volume fraction of 5% CO₂And culturing in a cell culture box with saturated humidity, changing the liquid for 1 time every 3d, and carrying out passage when the cell fusion degree reaches 70-80%, wherein all the cells used in the experiment are 3-5 generations.

The detection method comprises the following steps:

taking cells in logarithmic growth phase, adjusting cell density to 1 × 10⁴one/mL, seeded in 96-well plates at 200. mu.L per well. A blank control group and a dosing group are arranged, and each group is provided with 3 parallel holes.

After adding the reagent, incubation is continued for 24h, 20 mu L of 5mg/mL tetramethyl azozolium (MTT) solution is added into each well, after incubation for 4h, the solution is poured out, 150 mu L of dimethyl sulfoxide (DMSO) is added, shaking is carried out on a shaking table for 10min, and the D (lambda) value of each well at the wavelength of 450nm is detected by a microplate reader.

Cell proliferation rate (%) [ D (λ) experimental group/D (λ) blank control group ]. multidot.100%

Cell proliferation inhibition (%) of 100%

Sample concentration: the cell proliferation rate of the test sample is 0.1 percent, and the cell proliferation inhibition rate is 0.5 percent.

As can be seen from the test data in Table 1, compared with comparative examples 1-3, the production cycle of examples 1-3 was greatly shortened, the energy consumption was greatly reduced, the water consumption was greatly reduced, and the extraction rate and bioavailability of the active substances in ginseng were significantly improved. The method is characterized in that a differential gradient extraction and extraction concentration fermentation integrated mode is adopted, active substances in ginseng are comprehensively and fully extracted and synchronously transferred, water resources and energy are comprehensively utilized to the maximum extent, comparative example 1 and comparative example 2 are only conventional extraction and conventional gradient extraction, namely the concentration gradient degree of ethanol aqueous solution at each stage is small, the active substances of the ginseng cannot be comprehensively and fully extracted, and the processes are performed in a segmented mode, so that the extraction rate and the bioavailability are greatly reduced, and the connection time of the steps is long. Comparative example 3 does not carry out differential gradient extraction and enzymolysis, so that the medicinal materials can not be fully extracted and are fermented by microorganisms, and cellulose can not be decomposed to be used as a carbon source, so that the extraction rate is reduced, and the solid waste rate is increased.

Compared with comparative examples 1-2, the ethanol dosage in examples 1-3 is also obviously reduced, which shows that the ethanol dosage and the water consumption can be greatly saved and the cost is reduced on the basis of producing high-quality ginseng ferment by adopting the production integrated system.

Compared with comparative examples 1-2 and 4-6, the extraction rate of examples 1-3 is greatly improved; compared with comparative examples 1-6, the solid waste rate of examples 1-3 is greatly reduced; compared with comparative examples 1 to 7, the total amount of rare saponins and the proliferation rate of fibroblasts and the inhibition rate of scar cells in examples 1 to 3 are greatly increased, which indicates that the production conditions and parameters of the application are optimal; the enzymolysis process is added in the extraction process, so that the extraction rate can be improved, the solid waste rate can be reduced, and the bioavailability of the product can be improved.

TABLE 1 test results

The above description is only a preferred embodiment of the present application, and the protection scope of the present application is not limited to the above embodiments, and all technical solutions belonging to the idea of the present application belong to the protection scope of the present application. It should be noted that several improvements and modifications to the present application without departing from the principles of the present application will occur to those skilled in the art, and such improvements and modifications should also be considered within the scope of the present application.

Claims

1. A production integrated system of ginseng ferment is characterized by comprising an ethanol heating storage tank, a condensate storage tank, an extraction tank, a hot water recovery tank, a fermentation tank, a condenser, a cooler, an enzyme mixing tank, a heater, a gas-liquid separator and an alcohol-water mixing tank;

the alcohol-water mixed liquor at the bottom of the enzyme mixing tank is connected with an enzyme mixed liquor inlet pipeline arranged at the top of the extraction tank through a valve F1 by a pipeline;

the filtering pore diameter of the pipeline filter is 100-200 nm;

2. The ginseng ferment production integration system of claim 1, wherein a turning plate distributor is arranged at an upper portion inside the extraction tank, a connecting rod extends downwards from a bottom of a kettle cover of the extraction tank, a top end of the connecting rod is fixed with the kettle cover of the extraction tank, a bottom end of the connecting rod is hinged with a top surface of the turning plate distributor, baffles are arranged on two sides of the turning plate distributor, one baffle is arranged at a top end of the turning plate distributor and fixed with an inner wall of the extraction tank, the other baffle is arranged at a bottom end of the turning plate distributor and fixed with the inner wall of the extraction tank, and the turning plate distributor is horizontal in an unproduced state.

3. A method for producing ginseng ferment by using the integrated system for producing ginseng ferment of claim 1, comprising the following steps:

the concentrated solution and the human body ferment are obtained by the process comprising the following steps:

the filtering pore diameter of the pipeline filter is 100-200 nm;

the seed liquid is obtained by the following preparation steps:

4. The method for producing ginseng ferment of claim 3, wherein the constant temperature water in the hot water reflux tank is introduced into the fermentation tank jacket through a valve F23 and a fourth transfer pump, and then is refluxed into the hot water reflux tank from the fermentation tank jacket, thereby controlling the fermentation temperature in the fermentation tank at 37 ℃;

5. The method for producing ginseng ferment of claim 3, wherein the liquid MRS medium is composed of the following raw material components in parts by weight: 10 parts of peptone, 5 parts of beef extract powder, 4 parts of yeast powder, 20 parts of glucose, 2 parts of dipotassium hydrogen phosphate, 2 parts of triammonium citrate, 5 parts of sodium acetate, 0.2 part of magnesium sulfate, 0.05 part of manganese sulfate and 801 parts of tween; the pH value of the liquid MRS culture medium is 6.2 +/-0.2;

6. The ginseng ferment obtained by the method for producing ginseng ferment according to any one of claims 3 to 5.

7. The ginsenoside of claim 6, comprising 1000-1500mg of 20(S) ginsenoside Rg3, 500-1000mg of 20(R) ginsenoside Rg3 and 200-400mg of ginsenoside Rh2 per liter.

8. Use of the ginseng ferment of claim 6, in the preparation of cosmetics or ointments for promoting fibroblast proliferation or inhibiting scar cell proliferation, wherein the cosmetics or ointments comprise water, milk, cream or essence.

9. The use of the ginseng ferment of claim 8, wherein the ginseng ferment is added to the cosmetic in an amount of 0.1 to 2% by weight.