CN112851754B - 一种萃取-反萃取分离纯化surfactin方法 - Google Patents
一种萃取-反萃取分离纯化surfactin方法 Download PDFInfo
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- CN112851754B CN112851754B CN202110013345.0A CN202110013345A CN112851754B CN 112851754 B CN112851754 B CN 112851754B CN 202110013345 A CN202110013345 A CN 202110013345A CN 112851754 B CN112851754 B CN 112851754B
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- AFWTZXXDGQBIKW-UHFFFAOYSA-N C14 surfactin Natural products CCCCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 AFWTZXXDGQBIKW-UHFFFAOYSA-N 0.000 title claims abstract description 88
- NJGWOFRZMQRKHT-UHFFFAOYSA-N surfactin Natural products CC(C)CCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-UHFFFAOYSA-N 0.000 title claims abstract description 88
- NJGWOFRZMQRKHT-WGVNQGGSSA-N surfactin C Chemical compound CC(C)CCCCCCCCC[C@@H]1CC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-WGVNQGGSSA-N 0.000 title claims abstract description 88
- 238000000605 extraction Methods 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000000926 separation method Methods 0.000 title claims description 19
- 238000000746 purification Methods 0.000 title claims description 18
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 75
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 63
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 46
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims abstract description 41
- 239000000706 filtrate Substances 0.000 claims abstract description 25
- 239000012071 phase Substances 0.000 claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 15
- 239000002253 acid Substances 0.000 claims abstract description 13
- 239000012074 organic phase Substances 0.000 claims abstract description 13
- 230000001105 regulatory effect Effects 0.000 claims abstract description 10
- 239000012264 purified product Substances 0.000 claims abstract description 7
- 238000004108 freeze drying Methods 0.000 claims abstract description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 69
- 235000019441 ethanol Nutrition 0.000 claims description 35
- 239000002244 precipitate Substances 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 239000006228 supernatant Substances 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 239000003960 organic solvent Substances 0.000 abstract description 10
- 150000001875 compounds Chemical class 0.000 abstract description 7
- 239000008346 aqueous phase Substances 0.000 abstract description 6
- 230000002378 acidificating effect Effects 0.000 abstract description 4
- 230000007935 neutral effect Effects 0.000 abstract description 4
- 239000013049 sediment Substances 0.000 abstract description 4
- 238000010364 biochemical engineering Methods 0.000 abstract description 2
- 238000009210 therapy by ultrasound Methods 0.000 abstract 1
- 239000000284 extract Substances 0.000 description 10
- 238000011084 recovery Methods 0.000 description 10
- 239000007787 solid Substances 0.000 description 10
- 239000000843 powder Substances 0.000 description 8
- 244000063299 Bacillus subtilis Species 0.000 description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 108010028921 Lipopeptides Proteins 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 239000012535 impurity Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 238000003916 acid precipitation Methods 0.000 description 3
- 238000007670 refining Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000013375 chromatographic separation Methods 0.000 description 2
- 239000000287 crude extract Substances 0.000 description 2
- 230000032798 delamination Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000005189 flocculation Methods 0.000 description 2
- 230000016615 flocculation Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000002390 rotary evaporation Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 108010069514 Cyclic Peptides Proteins 0.000 description 1
- 102000001189 Cyclic Peptides Human genes 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000000622 liquid--liquid extraction Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000002537 thrombolytic effect Effects 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
萃取体系a(体积比) | pH | |
实施例3 | 正己烷:乙酸乙酯:甲醇:水=1:1:1:1 | 1.5 |
实施例4 | 正己烷:乙酸乙酯:甲醇:水=6:1:6:1 | 1.5 |
实施例5 | 正己烷:乙酸乙酯:甲醇:水=1:2:1:1 | 1.5 |
实施例6 | 正己烷:乙酸乙酯:甲醇:水=1:1:1:1 | 2.0 |
实施例7 | 正己烷:乙酸乙酯:甲醇:水=6:1:6:1 | 2.0 |
实施例8 | 正己烷:乙酸乙酯:甲醇:水=1:2:1:1 | 2.0 |
实施例9 | 正己烷:乙酸乙酯:甲醇:水=1:1:1:1 | 4.0 |
实施例10 | 正己烷:乙酸乙酯:甲醇:水=6:1:6:1 | 4.0 |
实施例11 | 正己烷:乙酸乙酯:甲醇:水=1:2:1:1 | 4.0 |
surfactin质量(mg/100mL醇过滤液) | surfactin回收率(%) | surfactin含量(%) | |
实施例3 | 264.18 | 97.34 | 35.98 |
实施例4 | 263.74 | 97.18 | 35.94 |
实施例5 | 263.91 | 97.24 | 35.96 |
实施例6 | 264.33 | 97.39 | 36.01 |
实施例7 | 264.01 | 97.28 | 35.97 |
实施例8 | 263.77 | 97.19 | 35.94 |
实施例9 | 264.23 | 97.36 | 36.02 |
实施例10 | 263.83 | 97.21 | 35.94 |
实施例11 | 263.86 | 97.22 | 35.91 |
Claims (4)
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CN113248571B (zh) * | 2021-06-30 | 2022-10-18 | 华东理工大学 | 利用热响应型聚合物npe-108分离纯化杆菌肽的方法 |
CN114106082A (zh) * | 2021-11-03 | 2022-03-01 | 南京理工大学 | 一种生物表面活性剂脂肽的分离纯化方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105001303A (zh) * | 2015-06-29 | 2015-10-28 | 山东华辰生物科技有限公司 | 一种利用低温冷冻环境从抗菌脂肽溶液中除盐的方法 |
CN108865928A (zh) * | 2018-06-13 | 2018-11-23 | 铜仁职业技术学院 | 一种枯草芽孢杆菌及用于制备黄荆子微生态制剂的方法 |
CN110790826A (zh) * | 2019-11-15 | 2020-02-14 | 江南大学 | 一种分离纯化脂肽类surfactin家族化合物的方法 |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN105001303A (zh) * | 2015-06-29 | 2015-10-28 | 山东华辰生物科技有限公司 | 一种利用低温冷冻环境从抗菌脂肽溶液中除盐的方法 |
CN108865928A (zh) * | 2018-06-13 | 2018-11-23 | 铜仁职业技术学院 | 一种枯草芽孢杆菌及用于制备黄荆子微生态制剂的方法 |
CN110790826A (zh) * | 2019-11-15 | 2020-02-14 | 江南大学 | 一种分离纯化脂肽类surfactin家族化合物的方法 |
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Effective date of registration: 20240513 Address after: 201615 14 new building, 1 new building, nine new highway, Jiuting Town, Songjiang District, Shanghai, China, 1 Patentee after: Shanghai Changwei Industrial Co.,Ltd. Country or region after: China Address before: 554300 Tongren Vocational and technical college, Chuandong Education Park, Bijiang District, Tongren City, Guizhou Province Patentee before: TONGREN POLYTECHNIC College Country or region before: China |