CN1128227C - Process for preparing high-purity malt sugar by multi-enzyme cooperative saccharification - Google Patents
Process for preparing high-purity malt sugar by multi-enzyme cooperative saccharification Download PDFInfo
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- CN1128227C CN1128227C CN 01130028 CN01130028A CN1128227C CN 1128227 C CN1128227 C CN 1128227C CN 01130028 CN01130028 CN 01130028 CN 01130028 A CN01130028 A CN 01130028A CN 1128227 C CN1128227 C CN 1128227C
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- maltose
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Abstract
The present invention relates to a method for preparing high purity maltose by multienzyme cooperative saccharification, which comprises that starch milk is liquified; liquified starch liquid is carried out with multi-enzyme cooperative saccharification; the saccharified liquid is refined into malt syrup; aiming at the insufficiency of low contents for single enzyme and double enzyme liquified starch liquid maltose at present, several kinds of enzymes suitable for preparing maltose are screened out. Malt syrup is prepared by multienzyme cooperative saccharification. The maltose content of the product is improved, and subsequent refining processes are simplified.
Description
Technical field
The present invention relates to the production method of maltose, be meant the method for preparing high-purity malt sugar by multi-enzyme cooperative saccharification in more detail.
Background technology
Maltose is one of glucose industry staple product, have characteristics such as sugariness gentleness, unique flavor, water absorbability are stable, also have excellent characteristic at aspects such as anti-brown stain, preservative property, thermostability, viscosity, be widely used as sweeting agent, humextant, crystallization inhibitor, stablizer, weighting agent etc. in industries such as food, fermentation, essence and flavoring agents.Because maltose has the character of putting glucose than the gentle slow release of Hyposmolality, accretion rate is slow than glucose, not participating in simultaneously the Regular Insulin carbohydrate metabolism just can absorb, medically, replace glucose preparation intravenous fluid to be difficult for causing blood sugar increasing with high purity maltose, be fit to very much the diabetic subject and use.
The production of maltose, the maltose of China history in existing more than 3,000 year so far before last thousand quincentenaries in Christian era.At present, the malt syrup of low-purity is mainly used in foodstuffs industry, and being of wide application of high purity maltose except that having the extensive use in food, fermentation, essence and flavoring agent industry, also is used widely at aspects such as health cares.
In recent years, domestic and international research and development to maltose concentrates on the following aspects: the preparation of the zymin that (1) thermotolerance is strong, vigor is high; (2) high maltose syrup is refining; (3) preparation of high purity maltose.Its final purpose all is in order to produce the high malt syrup of maltose content, to prepare high purity maltose then.The production of existing high purity maltose is the general malt syrup of producing earlier, and the multiple refining maltose content that obtains such as refining through absorption, ion exchange resin then, membrane sepn, crystallization reaches high purity maltose more than 98%.Because maltose content is lower, the highest about 80-85% in the malt syrup, causes follow-up refining complex process, has increased production cost greatly, has limited promoting the use of of high purity maltose to a great extent.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art part, a kind of production method of producing high purity maltose is provided.At present single enzyme and the low deficiency of double-enzyme hydrolysis liquefied starch maltose content, filter out the enzyme that the most suitable several maltose are produced, produce malt syrup with the multienzyme synergism saccharification, improve the maltose content of product, simplify follow-up process for refining.
The method of preparing high-purity malt sugar by multi-enzyme cooperative saccharification of the present invention comprises the following steps:
---starch milk liquefies;
---liquefied starch carries out the multienzyme synergism saccharification;
---saccharification liquid is through refining malt syrup.
The gained malt syrup can re-refine, concentrates, dry high purity maltose.
Described starch milk liquefies and is meant starch milk under the effect of starch liquefacation enzyme, and the mild hydrolysis that heats becomes the process of macromolecular substance such as dextrin, and the purpose of liquefaction is the effect of being convenient to follow-up saccharifying enzyme.
Top condition is that starch milk is controlled at 30～38%, and the pH value enters Jet liquefier 5.5～6.5 behind the adding Termamyl (high-temperature starch Ye Huamei, down together), liquefies at 100～105 ℃, and liquefier DE value is controlled at 6.5～9.
Described liquefied starch carries out the multienzyme synergism saccharification and is meant macromolecular substance hydrolysis generation maltose under the synergy of beta-amylase, Maltogenase (maltose generation enzyme, down together), Promozyme (debranching factor, down together) saccharifying enzyme such as dextrin.
Top condition is that the liquefied starch that goes out behind the enzyme carries out the multienzyme synergism saccharification, 0.9～1.8DP °/gram of adding beta-amylase dry starch, Maltogenase enzyme 5.2～7.8MANU/ gram dry starch, Promozyme enzyme 0.2～0.5PUN/ restrain dry starch, control pH value is 5.0～5.5,50～60 ℃ of temperature, saccharification 36～60 hours.
Refining pigment, the impurity that removes in the deglycation liquid that is meant of described saccharification liquid, to improve the purity of malt syrup, concrete process for purification is that charcoal absorption decolouring, ion exchange resin are refining.
The present invention compared with prior art has following outstanding advantage:
1. the present invention adopts the multienzyme synergism saccharification to be used for high maltose syrup production first, and product maltose content height, quality are good.
Existing high maltose syrup production technique all adopts single enzyme or double enzymes saccharification, and maltose content is the highest at 80-85%.The present invention adopts the multienzyme synergism saccharification to be used for high maltose syrup production first, and maltose content is up to 90-95% in the high maltose syrup.
2. high purity maltose work simplification, the production efficiency height, cost is low.
Existing high purity maltose production technique all is to adopt the double-enzyme method saccharification to produce malt syrup earlier, because maltose content is lower, the highest about 80-85%, cause follow-up refining complex process, that malt syrup needs is refining through adsorbents adsorb, ion exchange resin, the high purity maltose of multiple refining maltose content about 98% such as membrane sepn, crystallization.The refining step complexity has increased production cost greatly, has limited promoting the use of of high purity maltose to a great extent.Maltose content is up to 90-95% in the high maltose syrup product of the present invention, follow-up refining step is simplified greatly, single with the refining requirement that just can reach the preparation high purity maltose of membrane sepn, can effectively improve throughput, reduce cost, solved owing to adopt multiple refining step to cause the high difficult problem that is difficult to promote the use of of high purity maltose product cost.
Description of drawings
Fig. 1 is the process flow sheet of the method for preparing high-purity malt sugar by multi-enzyme cooperative saccharification of the present invention.
Embodiment
The invention will be further described in conjunction with following embodiment and accompanying drawing thereof.
Embodiment one
As shown in Figure 1, this technical process is a continuous production processes, and starch material adds water and transfers breast, and controlling concn is 30%, and pH value 5.5 enters Jet liquefier behind the adding Termamyl high temperature Ye Huamei, liquefies at 100 ℃, and the final DE value of liquefier is controlled at 6.5.Heat-treat the enzyme that goes out then, the liquefier that goes out behind the enzyme carries out the multienzyme synergism saccharification, and control pH value is 5.0,60 ℃ of temperature, 0.9DP °/gram of adding beta-amylase dry starch, Maltogenase enzyme 5.2MANU/ gram dry starch, Promozyme enzyme 0.2PUN/ restrain dry starch, saccharification 60 hours.Saccharification liquid through activated carbon decolorizing, ion exchange resin refining high maltose syrup, high maltose syrup is concentrated into 60-65% behind membrane sepn, spraying drying gets high purity maltose.Maltose content is 94.5% in the high maltose syrup that above-mentioned processing step obtains, and maltose content is 98.5% in the high purity maltose.
Embodiment two
Starch material adds water and transfers breast, and controlling concn is 35%, and pH value 6.0 enters Jet liquefier behind the adding Termamyl high temperature Ye Huamei, liquefies at 102 ℃, and the final DE value of liquefier is controlled at 7.5.Heat-treat the enzyme that goes out then, the liquefier that goes out behind the enzyme carries out the multienzyme synergism saccharification, and control pH value is 5.3,55 ℃ of temperature, 1.3DP °/gram of adding beta-amylase dry starch, Maltogenase enzyme 6.5MANU/ gram dry starch, Promozyme enzyme 0.35PUN/ restrain dry starch, saccharification 48 hours.Saccharification liquid through activated carbon decolorizing, ion exchange resin refining high maltose syrup, high maltose syrup is concentrated into 60-65% behind membrane sepn, spraying drying gets high purity maltose.Maltose content is 92.3% in the high maltose syrup that above-mentioned processing step obtains, and maltose content is 98.3% in the high purity maltose.
Embodiment three
Starch material adds water and transfers breast, and controlling concn is 38%, and pH value 6.5 enters Jet liquefier behind the adding Termamyl high temperature Ye Huamei, liquefies at 105 ℃, and the final DE value of liquefier is controlled at 9.Heat-treat the enzyme that goes out then, the liquefier that goes out behind the enzyme carries out the multienzyme synergism saccharification, and control pH value is 5.5,50 ℃ of temperature, 1.8DP °/gram of adding beta-amylase dry starch, Maltogenase enzyme 7.8MANU/ gram dry starch, Promozyme enzyme 0.5PUN/ restrain dry starch, saccharification 36 hours.Saccharification liquid through activated carbon decolorizing, ion exchange resin refining high maltose syrup, high maltose syrup is concentrated into 60-65% behind membrane sepn, spraying drying gets high purity maltose.Maltose content is 91.5% in the high maltose syrup that above-mentioned processing step obtains, and maltose content is 98.2% in the high purity maltose.
Claims (1)
1, a kind of method of preparing high-purity malt sugar by multi-enzyme cooperative saccharification is characterized in that comprising the following steps:
---starch milk liquefies, and is meant starch milk is controlled at 30～38%, and the pH value enters Jet liquefier 5.5～6.5 behind the adding high-temperature starch Ye Huamei, liquefies at 100～105 ℃, and liquefier DE value is controlled at 6.5～9;
---liquefied starch carries out the multienzyme synergism saccharification, be meant dextrin at beta-amylase, 0.9～1.8DP °/gram dry starch, maltose generate enzyme 5.2～7.8MANU/ gram dry starch, debranching factor 0.2～0.5PUN/ gram dry starch, control pH value 5.0～5.5, under 36～60 hours conditions of 50～60 ℃ of temperature, saccharification hydrolysis generation maltose;
---saccharification liquid gets high maltose syrup through activated carbon decolorizing, ion exchange resin refinement treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01130028 CN1128227C (en) | 2001-12-06 | 2001-12-06 | Process for preparing high-purity malt sugar by multi-enzyme cooperative saccharification |
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| CN 01130028 CN1128227C (en) | 2001-12-06 | 2001-12-06 | Process for preparing high-purity malt sugar by multi-enzyme cooperative saccharification |
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| CN1353200A CN1353200A (en) | 2002-06-12 |
| CN1128227C true CN1128227C (en) | 2003-11-19 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684504B (en) * | 2008-09-26 | 2013-10-30 | 保龄宝生物股份有限公司 | Preparation method of superhigh maltose syrup |
| CN101433283B (en) * | 2008-12-31 | 2011-11-23 | 保龄宝生物股份有限公司 | Syrup for roasting and preparation method thereof |
| CN101875954A (en) * | 2009-04-29 | 2010-11-03 | 山东福田药业有限公司 | Novel process for improving crystalline maltose yield |
| CN103205474B (en) * | 2013-04-24 | 2018-03-16 | 山东百龙创园生物科技股份有限公司 | A kind of preparation method for bakeing syrup |
| CN109234338A (en) * | 2018-09-08 | 2019-01-18 | 肇庆焕发生物科技有限公司 | A kind of production method of high purity crystal maltitol malt syrup |
| CN112029809A (en) * | 2020-09-14 | 2020-12-04 | 江苏省奥谷生物科技有限公司 | Method for producing high-purity maltose by multi-enzyme synergistic saccharification |
| CN112522346A (en) * | 2020-12-04 | 2021-03-19 | 江苏省奥谷生物科技有限公司 | Preparation method of high-purity oligomeric maltose |
| CN113215208A (en) * | 2021-05-26 | 2021-08-06 | 江苏省奥谷生物科技有限公司 | Preparation method of maltose powder with high maltose content |
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