CN112812943A - Bacterial cellulose culture room and use method thereof - Google Patents

Bacterial cellulose culture room and use method thereof Download PDF

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Publication number
CN112812943A
CN112812943A CN202110077451.5A CN202110077451A CN112812943A CN 112812943 A CN112812943 A CN 112812943A CN 202110077451 A CN202110077451 A CN 202110077451A CN 112812943 A CN112812943 A CN 112812943A
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culture
container
culture container
sealing door
room
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胡秀林
税朝毅
张维
张娟
吴力克
邓国宏
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Nanfang Hospital
First Affiliated Hospital of PLA Military Medical University
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First Affiliated Hospital of PLA Military Medical University
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Priority to CN202110077451.5A priority Critical patent/CN112812943A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a bacterial cellulose culture room and a using method thereof, which relate to the field of culture rooms and aim to achieve the aim, the technical scheme of the invention comprises a culture room body and a plurality of culture containers arranged in the culture room body; the culture chamber is provided with an outer sealing door, the culture container enters and exits the culture chamber body from the outer sealing door, and a disinfection device is arranged inside the culture chamber; the culture container is provided with an inner sealing door which can be controlled to open and close outside the culture room. The disinfection of the contrast test and the production of the product with the same quality can be realized simultaneously, the disinfection is more thorough, the air pollution is not easy to occur, the overall disinfection is sealed, and the culture result is more accurate and reliable.

Description

Bacterial cellulose culture room and use method thereof
Technical Field
The invention relates to a bacterial cellulose culture room and a using method thereof, and mainly relates to the field of culture rooms.
Background
Bacterial cellulose's cultivation, existing control experiment has batch production again, and whatever all needs the full-process disinfection pollution-free, otherwise any slight pollution will all influence final cultivation result, especially control sample, owing to will control the variable, accomplish real pollution-free experiment very hardly, and during batch production, for having satisfied same production environment, the environmental pollution takes place again easily when placing culture vessel and getting into same environment.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the bacterial cellulose culture chamber and the use method thereof, which can realize the comparison test and the disinfection of the production of products with the same quality at the same time, have more thorough disinfection, are not easy to cause air pollution, are globally disinfected and sealed, and have more accurate and reliable culture results.
In order to achieve the purpose, the technical scheme of the invention is as follows: comprises a culture chamber body and a plurality of culture containers arranged in the culture chamber body; the culture chamber is provided with a main oxygen adding pipe and an outer sealing door, the culture container enters and exits the culture chamber body from the outer sealing door, and a disinfection device is arranged inside the culture chamber; the culture container is provided with an inner sealing door which can be controlled to open and close outside the culture room.
The technical principle and the beneficial effects of the invention are as follows:
when the control page experiment is disinfected, firstly, the culture container is opened and placed in the culture chamber, the culture container and the culture chamber are communicated with each other, and the outer sealing door is closed; the culture chamber and the culture container can be simultaneously sterilized by the sterilizing device of the culture chamber; the culture vessel is closed and sealed at the outside. At this time, the culture container can be self-sealed through the inner sealing door, and can be mutually independent when being placed in the culture chamber, so that the variable quantity can be better controlled, and a control test can be conveniently carried out.
When cultivateing the product of same quality, the aforesaid operation follows, open the outer sealing door of cultivateing the room, because the inner sealing door of cultivateing the container has been closed, consequently, it is isolated state with the outside to cultivate the container, outside air can not lead to the fact the pollution to cultivate the container, in the material pours into the cultivation container into, place again and cultivate indoor, close outer sealing door, open degassing unit, the outside cultivation room space of cultivateing the container is disinfected, open the inner sealing door at last after the disinfection is accomplished, at this moment, cultivate container and cultivation room and communicate again, each cultivation container is in under same environment, be fit for the material of the same quality of large batch cultivation, the disinfection of whole process is very thorough, can not produce the pollution.
Can realize the disinfection of the production of contrast experiment and same quality product simultaneously through above this device to and the disinfection is more thorough, and the difficult air pollution's that appears situation, and the global disinfection is sealed, and the result of cultivateing is more accurate reliable.
Preferably, the two sides of the culture chamber corresponding to the culture containers are respectively provided with a sliding rail, the culture containers slide in and out of the culture chamber through the sliding rails, the culture containers are conveniently conveyed into the culture chamber, and meanwhile the culture containers are better placed.
Preferably, the culture container is provided with an injection hole and an oxygenation hole which are communicated with the inside, the side wall of the culture chamber is slidably matched with a plurality of injection pipes and a branch oxygenation pipe corresponding to the culture container, the front ends of the injection pipes point to the hole openings of the injection holes, the injection pipes are in plug fit after sliding to the hole openings of the injection holes, the rear ends of the injection pipes are positioned outside the culture chamber body and are communicated with the feeding device, the front ends of the branch oxygenation pipes point to the hole openings of the oxygenation hole, the branch oxygenation pipes are in plug fit after sliding to the hole openings of the oxygenation hole, the rear ends of the branch oxygenation pipes are positioned outside the culture chamber body and are communicated with the oxygenation device, the culture container is directly filled with culture substances outside the culture chamber through the injection pipes, the culture substances are prevented from being taken out of the culture container and added with the culture substances, can further avoid pollution in the process of adding culture substances, and the culture result is more reliable.
Preferably, the inner wall of the culture chamber is provided with a quick connector for a circuit corresponding to each culture container, when the culture containers enter the culture chamber, the circuits of the culture containers are communicated through the quick connectors, so that the circuits in the culture containers can be quickly and conveniently communicated, more electric components can be conveniently added in the culture containers, the parameters of the culture containers can be more automatically controlled, and the culture containers and the culture chamber can be simultaneously ensured to be mutually independently communicated.
Preferably, the quick connector is a magnetic connector, so that the circuit of the culture container can be conveniently and conveniently communicated.
Preferably, the inner sealing door comprises a fixed ring, a substrate and a sealing plate, the substrate is arranged in the fixed ring, a stud is arranged in the middle of the substrate, the substrate is provided with a plurality of lower window regions by taking the stud as a circle center, the fixed ring is detachably fixed at a port of the culture container, the middle of the sealing plate is in threaded fit with the stud, the sealing plate is driven by a motor to rotate, and the sealing plate is provided with an upper window region and a sealing region corresponding to the lower window regions; when the sealing plate rotates to be close to the substrate, the sealing area is folded to the lower window area to plug the port of the culture container; when the sealing plate rotates away from the substrate, the upper window area and the lower window area are gradually overlapped to open the port of the culture container.
Because the closing plate cooperation is on the double-screw bolt, the double-screw bolt is fixed on the base plate simultaneously, therefore the closing plate is at the pivoted in-process on the double-screw bolt, the closing plate will keep away from or be close to the base plate, when the closing plate is close to the base plate, the sealing area will aim at lower window district, and then with the lower window district shutoff of base plate, so that will cultivate container and cultivateing the room and separate, when the base plate was kept away from to the closing plate, the sealing area will stagger the separation with lower window district, lower window district is opened, cultivate container and cultivateing the room intercommunication. Can be very convenient realization sealed to culture container through above to adopt closing plate and double-screw bolt screw-thread fit's mode, can realize the regulation to the leakproofness through adjusting closing plate pivoted angle, change sealed intensity.
Preferably, the outer fringe of closing plate is equipped with circular teeth of a cogwheel, the motor set up in culture container, the output shaft of motor be equipped with the major axis gear of circular teeth of a cogwheel meshing, also axial displacement when the closing plate rotates, consequently adopt major axis gear can adapt to closing plate axial displacement, can be more convenient the rotation of the outside control closing plate of culture chamber through the motor.
Preferably, the culture chamber is provided with an external container sensor, and each culture container is provided with an internal container sensor, so that various parameters of the culture chamber and the culture containers can be detected conveniently.
Preferably, the sensor outside the container and the sensor inside the container comprise a temperature and humidity sensor, an oxygen sensor and an air pressure sensor, so that the temperature, humidity and air pressure of the culture chamber and the culture container can be conveniently detected.
A method for using a bacterial cellulose culture room comprises the following steps:
d1 placing the culture container with the inner sealing door opened in the culture chamber, opening the inner sealing door, keeping the culture device communicated with the culture chamber, and closing the outer sealing door;
d2 starting the disinfection device to disinfect the culture room and the culture container;
d3 closing the inner sealing door under the state that the outer sealing door is kept closed;
d4 adding nutrient substances into the culture container comprises the following two operation modes:
d4.1, opening the outer sealing door, taking out the culture container, and adding nutrient substances into the culture container through the orifice of the injection hole; putting the culture container into the culture chamber again, closing the upper outer sealing door, starting the disinfection device, and disinfecting the culture chamber space outside the culture container;
d4.2 directly driving the front end of the injection tube to be communicated with the orifice of the injection hole, and adding the culture substance into the culture container through the injection tube.
Through the method, the sterilization of a contrast test or the production of products with the same quality can be realized, the sterilization is more thorough, the air pollution situation is not easy to occur, the overall sterilization is sealed, and the culture result is more accurate and reliable.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments will be briefly introduced below, it is obvious that the drawings in the following description are only two of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a side cross-sectional view of an embodiment of the present invention;
FIG. 2 is a side sectional view of a culture vessel according to an embodiment of the present invention;
FIG. 3 is a plan view of a culture vessel according to an embodiment of the present invention.
The device comprises a culture chamber 1, a culture container 2, a base plate 3, a sealing plate 4, a stud 5, an outer sealing door 6, a motor 7, a slide rail 8, a quick connector 9, a sealing sleeve 10, an injection pipe 11, a fixing ring 12, a long shaft gear 13, an upper window area 14, a lower window area 15 and a sealing device 16.
Detailed Description
The technical solutions in the present invention will be described clearly and completely with reference to the accompanying drawings, and it is to be understood that the described embodiments are merely preferred embodiments of the present invention, rather than all embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Examples
As shown in FIG. 1, the embodiment of the present invention comprises a main body of a culture chamber 1 and a plurality of culture vessels 2 provided inside the main body of the culture chamber 1.
The culture room 1 is provided with a main oxygen adding pipe and an outer sealing door 6, the culture container 2 enters and exits the culture room 1 body from the outer sealing door 6, and a disinfection device is arranged inside the culture room 1; the culture container 2 is provided with an inner sealing door which can be controlled to open and close outside the culture chamber 1.
When the control page experiment is disinfected, firstly, the culture container 2 is opened and placed in the culture room 1, the culture container 2 and the culture room 1 are communicated with each other, and the outer sealing door 6 is closed; the culture chamber 1 and the culture vessel 2 can be sterilized at the same time by the sterilizing device of the culture chamber 1; the culture vessel 2 is closed and sealed at the outside. At this time, the culture vessel 2 is self-sealed by the inner sealing door and is placed in the culture chamber 1 independently of each other, so that a control test can be performed.
When the same quality product is cultivated, then, the operation is carried out, the outer sealing door 6 of the cultivation room 1 is opened, the inner sealing door of the cultivation container 2 is closed, the cultivation container 2 is isolated from the outside, the outside air can not pollute the cultivation container 2, the cultivation material is injected into the cultivation container 2 and then placed into the cultivation room 1, the outer sealing door 6 is closed, the sterilizing device is opened, the space of the cultivation room 1 outside the cultivation container 2 is sterilized, the inner sealing door is opened finally after the sterilization is finished, at the moment, the cultivation container 2 is communicated with the cultivation room 1 again, each cultivation container 2 is in the same environment, the method is suitable for cultivating the same quality material in large batch, the whole process is quite thorough in sterilization, and pollution can not be generated.
Can realize the disinfection of the production of contrast experiment and same quality product simultaneously through above this device to and the disinfection is more thorough, and the difficult air pollution's that appears situation, and the global disinfection is sealed, and the result of cultivateing is more accurate reliable.
The two sides of the culture chamber 1 corresponding to the culture containers 2 are respectively provided with a slide rail 8, the culture containers 2 slide in and out through the slide rails 8 to the culture chamber 1, so that the culture containers 2 are conveniently conveyed into the culture chamber 1, and meanwhile, the culture containers 2 are better placed.
The culture container 2 is provided with an injection hole and an oxygenation hole which are communicated with the inside, the side wall of the culture chamber 1 is provided with a plurality of injection pipes 11 and branch oxygenation pipes corresponding to the culture container 2 in a sliding fit mode, and the injection pipes 11 and the culture chamber 1 are in sliding fit through a sealing sleeve 10. The front end of the injection pipe 11 points to the orifice of the injection hole, the injection pipe 11 is in plug fit after sliding towards the orifice of the injection hole, the rear end of the injection pipe 11 is positioned outside the culture chamber 1 body and is communicated with the feeding device, the front end of the branch oxygen adding pipe points to the orifice of the oxygen adding hole, the branch oxygen adding pipe is in plug fit after sliding towards the orifice of the oxygen adding hole, the rear end of the branch oxygen adding pipe is positioned outside the culture chamber (1) body and is communicated with the oxygen adding device, so that the culture container 2 can be directly filled with culture substances outside the culture chamber 1 through the injection pipe 11, the culture container 2 is prevented from being taken out and the culture substances are prevented from being added, the pollution in the process of adding the culture substances can be further avoided, and the culture result is more reliable.
The inner wall of the culture chamber 1 is provided with a quick connector 9 for a circuit corresponding to each culture container 2, when the culture containers 2 enter the culture chamber 1, the circuits of the culture containers 2 are communicated through the quick connectors 9, so that the circuits can be quickly connected into the culture containers 2, more electric components can be conveniently added into the culture containers 2, the parameters of the culture containers 2 can be more automatically controlled, and the culture containers 2 and the culture chamber 1 can be simultaneously ensured to be communicated independently. The quick connector 9 is a magnetic connector, so that the circuit of the culture container 2 can be conveniently communicated.
The inner sealing door comprises a fixing ring 12, a substrate 3 and a sealing plate 4, wherein the substrate 3 is arranged in the fixing ring 12, a stud 5 is arranged in the middle of the substrate 3, a plurality of lower window areas 15 are arranged on the substrate 3 by taking the stud 5 as a circle center, the fixing ring 12 is detachably fixed at a port of the culture container 2, the middle of the sealing plate 4 is in threaded fit with the stud 5, the sealing plate 4 is driven by a motor 7 to rotate, and the sealing plate 4 is provided with an upper window area 14 and a sealing area corresponding to the window; when the sealing plate 4 rotates to be close to the substrate 3, the sealing area is folded to the lower window area 15 to seal the port of the culture container 2; when the sealing plate 4 is rotated away from the substrate 3, the upper window region 14 and the lower window region 15 gradually overlap to open the port of the culture vessel 2.
Because the cooperation of closing plate 4 is on the double-screw bolt 5, double-screw bolt 5 is fixed on base plate 3 simultaneously, consequently closing plate 4 is at the pivoted in-process on double-screw bolt 5, closing plate 4 will keep away from or be close to base plate 3, when closing plate 4 is close to base plate 3, lower window district 15 will be aimed at to the sealing area, and then lower window district 15 shutoff with base plate 3, so that keep apart culture container 2 and culture room 1, when closing plate 4 keeps away from base plate 3, the sealing area will stagger the separation with lower window district 15, lower window district 15 is opened, culture container 2 communicates with culture room 1. Can be very convenient realization sealed to culture container 2 through above to adopt closing plate 4 and 5 screw thread complex modes of double-screw bolt, can realize the regulation to the leakproofness through adjusting closing plate 4 pivoted angle, change sealed intensity.
The outer edge of the lower window area 15 is provided with a sealing ring, and a sealing structure is formed when the sealing plate 4 is sealed and the sealing plate 16 is closed.
The outer fringe of closing plate 4 is equipped with the circular teeth of a cogwheel, motor 7 set up in culture container 2, this motor 7 is servo motor 7, the output shaft of motor 7 be equipped with circular teeth of a cogwheel engaged long axis gear 13, also at axial displacement when closing plate 4 rotates, consequently adopt long axis gear 13 can adapt to closing plate 4 axial displacement, can be more convenient the rotation of 1 external control closing plate 4 in cultivation room through motor 7.
The culture chamber 1 is provided with an outer container sensor, and each culture container 2 is respectively provided with an inner container sensor, so that various parameters of the culture chamber 1 and the culture containers 2 can be conveniently detected. The sensor outside the container and the sensor inside the container comprise a temperature and humidity sensor (model THT-N163A), an oxygen sensor (model class B-2C) and a gas pressure sensor (model MPX10), so that the temperature and humidity and the gas pressure of the culture chamber 1 and the culture container 2 can be conveniently detected.
A method for using a bacterial cellulose culture room comprises the following steps:
d1 placing culture container 2 with inner sealing door opened in culture chamber 1, opening the inner sealing door, keeping the culture device and culture chamber 1 connected, and closing outer sealing door 6;
d2 starting the disinfection device to disinfect the culture room 1 and the culture container 2;
d3 closing the inner sealing door with the outer sealing door 6 kept closed;
d4 adding nutrients to culture container 2, comprising the following two modes of operation:
d4.1, opening the outer sealing door 6, taking out the culture container 2, and then adding nutrient substances into the culture container 2 through the orifice of the injection hole; putting the culture container 2 into the culture chamber 1 again, closing the upper outer sealing door 6, starting the disinfection device, and disinfecting the culture chamber 1 space outside the culture container 2;
d4.2 directly drives the tip of the injection tube 11 to communicate with the orifice of the injection hole, and the culture medium is added to the culture container 2 through the injection tube 11.
Through the method, the sterilization of a contrast test or the production of products with the same quality can be realized, the sterilization is more thorough, the air pollution situation is not easy to occur, the overall sterilization is sealed, and the culture result is more accurate and reliable.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (10)

1. A bacterial cellulose culture room is characterized by comprising a culture room (1) body and a plurality of culture containers (2) arranged in the culture room (1) body;
the culture chamber (1) is provided with a main oxygenation pipe and an outer sealing door (6), the culture container (2) enters and exits the culture chamber (1) body from the outer sealing door (6), and a disinfection device is arranged inside the culture chamber (1);
the culture container (2) is provided with an inner sealing door which can be controlled to open and close outside the culture chamber (1).
2. A bacterial cellulose cultivation room as claimed in claim 1, wherein: the two sides of the culture chamber (1) corresponding to the culture container (2) are respectively provided with a slide rail (8), and the culture container (2) slides in and out of the culture chamber (1) through the slide rails (8).
3. A bacterial cellulose cultivation room as claimed in claim 1, wherein: the side wall of the culture chamber (1) is provided with a plurality of injection pipes (11) and a branch oxygen adding pipe in a sliding fit mode corresponding to the culture container (2), the front ends of the injection pipes (11) point to the orifices of the injection holes, the injection pipes (11) are in insertion fit after sliding towards the orifices of the injection holes, the rear ends of the injection pipes (11) are located outside the culture chamber (1) body and are communicated with a feeding device, the front ends of the branch oxygen adding pipes point to the orifices of the oxygen adding holes, the branch oxygen adding pipes are in insertion fit after sliding towards the orifices of the oxygen adding holes, and the rear ends of the branch oxygen adding pipes are located outside the culture chamber (1) body and are communicated with an oxygen adding device.
4. A bacterial cellulose cultivation room as claimed in claim 3, wherein: the inner wall of the culture chamber (1) corresponds to each culture container (2) and is provided with a quick connector (9) for a circuit, and when the culture container (2) enters the culture chamber (1), the circuit of the culture container (2) is communicated through the quick connector (9).
5. A bacterial cellulose cultivation room as claimed in claim 4, wherein: the quick joint (9) is a magnetic joint.
6. A bacterial cellulose cultivation room as claimed in claim 4, wherein: the inner sealing door comprises a fixing ring (12), a substrate (3) and a sealing plate (4), the substrate (3) is arranged in the fixing ring (12), a stud (5) is arranged in the middle of the substrate (3), a plurality of lower window areas (15) are arranged on the substrate (3) by taking the stud (5) as a circle center, the fixing ring (12) is detachably fixed at a port of the culture container (2), the middle of the sealing plate (4) is in threaded fit with the stud (5), the sealing plate (4) is driven to rotate by a motor (7), and the sealing plate (4) is provided with an upper window area (14) and a sealing area corresponding to the lower window areas (15);
when the sealing plate (4) rotates to be close to the substrate (3), the sealing area closes the lower window area (15) to seal the port of the culture container (2);
when the sealing plate (4) rotates away from the substrate (3), the upper window region (14) and the lower window region (15) are gradually overlapped to open the port of the culture container (2).
7. A bacterial cellulose cultivation room as claimed in claim 6, wherein: the outer fringe of closing plate (4) is equipped with circular teeth of a cogwheel, motor (7) set up in culture container (2), the output shaft of motor (7) be equipped with circular teeth of a cogwheel meshing's major axis gear (13).
8. A bacterial cellulose cultivation room as claimed in claim 3, wherein: the culture chamber (1) is provided with a container external sensor, and each culture container (2) is provided with a container internal sensor.
9. A bacterial cellulose cultivation room as claimed in claim 8, wherein: the sensor outside the container and the sensor inside the container comprise a temperature and humidity sensor, an oxygen sensor and an air pressure sensor.
10. The method of using a bacterial cellulose cultivation room as claimed in claim 1, wherein: the method comprises the following steps:
d1 placing the culture container (2) with the inner sealing door opened in the culture room (1), opening the inner sealing door, keeping the culture device communicated with the culture room (1), and closing the outer sealing door (6);
d2 starting the disinfection device to disinfect the culture room (1) and the culture container (2);
d3 closing the inner sealing door under the state that the outer sealing door (6) is kept closed;
d4 adding nutrient substances into the culture container (2) comprises the following two operation modes:
d4.1, opening the outer sealing door (6), taking out the culture container (2), and adding nutrient substances into the culture container (2) through the orifice of the injection hole; putting the culture container (2) into the culture chamber (1) again, closing the upper outer sealing door (6), starting the disinfection device, and disinfecting the culture chamber (1) space outside the culture container (2);
d4.2 directly driving the front end of the injection tube (11) to communicate with the orifice of the injection hole, and adding the culture material into the culture container (2) through the injection tube (11).
CN202110077451.5A 2021-01-20 2021-01-20 Bacterial cellulose culture room and use method thereof Pending CN112812943A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110077451.5A CN112812943A (en) 2021-01-20 2021-01-20 Bacterial cellulose culture room and use method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110077451.5A CN112812943A (en) 2021-01-20 2021-01-20 Bacterial cellulose culture room and use method thereof

Publications (1)

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CN112812943A true CN112812943A (en) 2021-05-18

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113621491A (en) * 2021-08-09 2021-11-09 江苏国际旅行卫生保健中心(南京海关口岸门诊部) Bacteria culture device based on medical clinical laboratory is anti-pollution formula

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113621491A (en) * 2021-08-09 2021-11-09 江苏国际旅行卫生保健中心(南京海关口岸门诊部) Bacteria culture device based on medical clinical laboratory is anti-pollution formula
CN113621491B (en) * 2021-08-09 2023-08-01 江苏国际旅行卫生保健中心(南京海关口岸门诊部) Anti-pollution bacterial culture device based on medical clinical laboratory

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