CN112808187A - Automatic equipment and method for producing bubble capsule microbial inoculum - Google Patents
Automatic equipment and method for producing bubble capsule microbial inoculum Download PDFInfo
- Publication number
- CN112808187A CN112808187A CN202110067432.4A CN202110067432A CN112808187A CN 112808187 A CN112808187 A CN 112808187A CN 202110067432 A CN202110067432 A CN 202110067432A CN 112808187 A CN112808187 A CN 112808187A
- Authority
- CN
- China
- Prior art keywords
- electric
- tank
- way ball
- reaction
- storage tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
- B01J13/06—Making microcapsules or microballoons by phase separation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Sustainable Development (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Computer Hardware Design (AREA)
- Immunology (AREA)
- Manufacturing Of Micro-Capsules (AREA)
Abstract
The invention relates to the technical field of production of biological microcapsules, in particular to automatic equipment and a method for producing a bubble capsule microbial inoculum. The equipment device is a machine which can automatically complete the whole production preparation and disinfection process of the positive drop microcapsule microbial inoculum by utilizing a programmable controller, and is characterized in that the mass transfer of the positive drop capsule is improved by a bubble technology, so that the oxygen demand is well supported, and the equipment device is particularly suitable for manufacturing the microcapsule microbial inoculum with large oxygen demand; meanwhile, various parameters can be adjusted at any time, better repeatability is realized, the condition consistency is better, the automation degree is high, the microcapsule preparation can be automatically completed in one step and can be completed step by step, and technical support is provided for preparing the microcapsules in batches for a long time.
Description
Technical Field
The invention relates to the technical field of production of biological microcapsules, in particular to automatic equipment and a method for producing a bubble capsule microbial inoculum.
Background
Microcapsules are a technique in which a solid or liquid is encapsulated with a film material to form minute particles having a size in the micrometer or millimeter range. The microcapsule technology has the advantages that the formed microcapsule can protect the enclosed objects from the external environment, but can release the enclosed objects when the wall materials are damaged under proper conditions. Thus, substances which cannot coexist originally can be put into a product system to play a role under proper conditions. In agricultural production, microcapsules can be used in the production of certain chemical fertilizers. For example, the silicon used as the nutrient of crops is generally alkaline, and when the silicon is mixed with a nitrogen fertilizer, chemical reaction can occur to lose the nutritional functions of the silicon and the nitrogen fertilizer, so the silicon and the nitrogen fertilizer cannot be compounded, and the silicon and the nitrogen fertilizer can only be used independently in time sharing during fertilization, thereby increasing a large amount of cost and also hindering the popularization and application of silicon fertilizers;
the traditional positive dropping method for preparing microcapsules is to uniformly mix alginate and solute and then drop the mixture into calcium salt solution, calcium ions diffuse into the drops and react with the alginate to form solid gel spheres, and the solute is embedded in the gel. The back-drop method for preparing traditional microcapsule is to mix calcium salt and thickening agent, drop into alginate solution, and form a layer of gel film to wrap liquid core through calcium ion diffusion to the periphery and reaction with alginate, thus preparing hollow microcapsule. In the production process of the capsule product, compared with a back-drop method, the front-drop method has the advantages of lower manufacturing cost, simpler process design and easier realization of automatic control of the whole operation. However, because of the limitation of the inherent principle, the microcapsules prepared by the forward dropping method have inferior mass transfer performance compared with those prepared by the reverse dropping method, and particularly when used for microbial cell culture, the general forward dropping method has difficulty in satisfying the aerobic growth of the embedded cells due to the problem of oxygen transfer.
Disclosure of Invention
In order to solve the technical problems, the invention has the first purpose that a machine capable of automatically completing the whole production and preparation process of the positive drop microcapsule microbial inoculum is designed by utilizing a programmable controller, the mass transfer of a positive drop capsule is improved by a bubble technology, various parameters can be adjusted at any time, better repeatability is realized, the condition consistency is better, and technical support is provided for preparing microcapsules in batches for a long time; further, the second purpose of the invention is to provide a method for preparing the bubble microcapsule microbial inoculum.
In order to achieve the first object, the invention adopts the following technical scheme:
the invention relates to automation equipment for producing a bubble capsule microbial inoculum, which comprises a switch group (2) consisting of a plurality of switches, a programmable controller (4), a propulsion controller or an air compressor (1) and a relay (3) to form a control system; an operation system consists of a propeller (9), a propulsion tank (19), electric three-way ball valves (20) - (23), water pumps (5) and (11), an air pump (10) and a magnetic stirrer (6); a pipeline reaction system consists of a bubble stone (7), a dripper (8), liquid storage tanks (12-14, 18), a reaction tank (17), a filter barrel (16), a capsule storage tank (15) and a connecting water pipe in the middle;
the control system, the operation system and the pipeline reaction system form a complete automatic device;
in the running process of the program, the programmable controller (4) can select and change the running mode according to each button input signal provided by the switch group (2), realize the step-by-step running and the repeated circulating running of each step of capsule preparation, and meet the running requirements of different occasions;
the programmable controller (4) can send a starting signal to the propulsion controller (1) according to the requirement of a program and simultaneously receive a state signal from the propulsion controller (1), once the propulsion controller (1) is started, the forward and backward movement of the propeller (9) can be automatically controlled, and the forward and backward movement of a piston in the propulsion tank (19) is further controlled;
the programmable controller (4) can directly control the operation of the electric three-way ball valves (20) - (23) to realize the directional flow of liquid in different pipelines;
the programmable controller (4) can control the water pumps (5) and (11) with any voltage and the magnetic stirrer (6) through the relay (3);
the propeller (9) can be matched with and provided with propeller tanks (19) with various sizes and corresponding pistons;
the propelling tank (19) is connected with an electric three-way ball valve (23), receives the instruction of the programmable controller (3), changes the connecting direction of the three-way valve according to the requirement, allows liquid to be sucked into the propelling tank (19) from the liquid storage tank (18) in one way when in preparation, and allows the liquid to be dripped out from the propelling tank (19) through the dripper (8) when in liquid dripping;
the dripper (8) can select the aperture with different sizes of 1-4 mm;
the capsule storage tank (15) is a sealed tank which can be vacuumized, the sealed tank contains a filter vat (16), and the sealed tank is provided with a water inlet pipe and a water outlet pipe. The filter vat is used for collecting capsules, one end of the water inlet pipe is plugged into the filter vat (16), and the other end of the water inlet pipe is connected with the reaction tank (17); one end of the water outlet pipe is positioned outside the filter vat (16), and the other end is connected with an electric three-way ball valve (21);
stirring power is provided by the electromagnetic stirrer (6) at the bottom of the reaction tank (17), and a dripper (8) for dripping reaction liquid is arranged above the reaction tank (17), so that the function of dripping while stirring can be realized.
In order to achieve the second object, the technical scheme adopted by the invention comprises a disinfection method and a production method, and the method comprises the following specific steps:
the first method comprises the following steps:
1) transferring a water pipe head connected with the liquid storage tank (13) to the liquid storage tank (12), and transferring a water pipe head connected with the liquid storage tank (14) to the reaction tank (17), so that an inlet and outlet loop is formed around the liquid storage tank (12) and the reaction tank (17) during disinfection, and the disinfection solution can be recycled conveniently;
2) water is filled in the liquid storage tank (12) and ozone is introduced, the switch group (2) is set to be in a disinfection mode, and disinfection is started;
3) the programmable controller (4) controls the electric three-way ball valves (20) - (22) and the water pump (5) to pump ozone water from the liquid storage tank (12) into the reaction tank (17);
4) sterilisation of the circuit entering and exiting around the tank (12): the programmable controller (4) controls the electric three-way ball valves (20) - (22) and the water pump (5), and ozone water circulates in the loops of the liquid storage tank (12), the electric three-way ball valves (20) - (21), the water pump (5) and the electric three-way ball valve (22) for a plurality of minutes by utilizing the loops which go in and out around the liquid storage tank (12);
5) sterilisation of the circuit entering and exiting around the reaction tank (17): the programmable controller (4) controls the electric three-way ball valves (20) - (22) and the water pump (5) to enable ozone water to enter loops of the electric three-way ball valve (21), the water pump (5), the electric three-way ball valve (22), the reaction tank (17) and the capsule storage tank (15) from the liquid storage tank (12) through the electric three-way ball valve (20) for circulation for a plurality of minutes;
6) the programmable controller (4) controls the electric three-way ball valves (21) - (22) and the water pump (5) to discharge ozone water from the reaction tank (17) to the liquid storage tank (12) for reozonation through a loop which passes in and out of the capsule storage tank (15) and the electric three-way ball valve (20) and surrounds the liquid storage tank (12);
7) repeating the steps 3) -6) for a plurality of times to complete the disinfection of the pipelines and each liquid storage tank, and restoring each pipeline to the original position.
Secondly, the production method comprises the following steps:
1) preparing reaction liquids a and B: preparing solution A and solution B with required concentration, and respectively adding into the liquid storage tanks (14) and (18);
preferably, the main component of the reaction liquid A is a calcium salt solution, and the main component of the reaction liquid B is an alginate solution;
2) designing and starting a programmable controller: designing a program of a programmable controller (4), starting a switch in a switch group (2), starting the programmable controller (4) to send signals to adjust the steering of ball valves (20) - (22), simultaneously starting a relay (3) to start a water pump (5), pumping a certain amount of reaction liquid A in a liquid storage tank (14) into a reaction tank (17), and starting a magnetic stirrer (6); meanwhile, the programmable controller (4) starts the propulsion controller (1) to enable the propeller (9) to retreat to drive the piston in the propulsion tank (19) to retreat, and the reaction liquid B in the liquid storage tank (18) is sucked into the propulsion tank (19) through the electric ball valve (23);
3) preparing a bubble stone: starting a water pump (11) by using a programmable controller (4), humidifying the air bubble stone (7) for 10-30 s, and then starting an air pump (10) to blow air to the air bubble stone (7);
4) preparing microcapsules: the programmable controller (4) adjusts the rotation direction of the electric three-way ball valve (23) according to a feedback signal of the propulsion controller (1), then sends a signal to the propulsion controller (1), the propulsion controller (1) adjusts the propeller (9) to push the piston in the propulsion tank (19) to advance, so that the reaction liquid B is dripped into the slit of the bubble stone (7) through the electric three-way ball valve (23) and the dripper (8) to generate a bubble ball, and then is dripped into the reaction tank (17) to react with the reaction liquid A under the stirring of the magnetic stirrer (6) to form a solid capsule. After the propulsion is finished, the reaction is continued for a plurality of minutes under the magnetic stirring according to the requirement, so that the reaction is completely finished;
5) and (3) recovering microcapsules: the programmable controller (4) adjusts the electric three-way ball valves (21) - (22) and starts the water pump (5), so that liquid in the reaction tank (17) forms circulation among the filter vat (16), the capsule storage tank (15), the electric three-way ball valve (21), the water pump (5), the electric three-way ball valve (22) and the reaction tank (17), and finally the capsule is intercepted in the filter vat (16);
6) microcapsule cleaning: the programmable controller (4) adjusts the electric three-way ball valves (20) - (22), starts the water pump (5), pumps the cleaning agent in the liquid storage tank (12) into the reaction tank (17), and repeats the step 5) to clean the capsule; then the programmable controller (4) adjusts the electric three-way ball valve (22), turns on the water pump (5), and discharges the liquid as waste liquid into the liquid storage tank (13);
7) and (3) automatically and repeatedly executing: the programmable controller (4) automatically repeats the steps 2) to 6) according to the preset repetition times; after the repetition times are finished, the capsule storage tank (15) is opened, and the capsules in the filter barrel (16) are taken out.
The existing method for preparing the calcium alginate capsule by the positive dropping method is usually completed by a manual method, and is difficult to prepare on a large scale. The automation equipment and the method for producing the bubble capsule microbial inoculum have compact structure and reasonable design, form the processes of disinfection, cyclic preparation and recovery of production pipelines, and can carry out an unattended automatic working mode. In addition, the invention introduces the concept of bubble capsules, and a large amount of bubbles are arranged in the formed positive dropping capsules, thereby greatly improving the air mass transfer effect and leading the embedded aerobic microorganisms to grow better.
Drawings
FIG. 1 is a schematic diagram of the structure and flow of the present invention;
in the figure, 1, a propulsion controller or an air compressor; 2. a switch group; 3. a relay; 4. a programmable controller; 5. a water pump; 6. a magnetic stirrer; 7. a bubble stone; 8. a dripper; 9. a propeller or a dispenser; 10. an air pump; 11. a water pump; 12-14. a liquid storage tank; 15. a capsule storage tank; 16. a filter vat; 17. a reaction tank; 18. a liquid storage tank; 19. a propulsion tank or dispensing valve; 20-23, electric three-way ball valve;
in the figure, thin line connections represent electric wires, and diagonal thick line connections represent water pipes.
Detailed Description
The present invention will be described in further detail with reference to the following description and examples. The following examples are not intended to limit the invention.
Example 1 example of the apparatus
As shown in fig. 1, the automatic apparatus for producing a bubble capsule bacterial agent of the present invention is a device composed of a control system, an operation system, and a pipeline reaction system, and is specifically described as follows:
1. the control system comprises a programmable controller (4), a propulsion controller or an air compressor (1), a switch group (2) and a relay (3), wherein the switch group (2) is connected to the input end of the programmable controller (4), the programmable controller (4) is simultaneously connected with the propulsion controller or the air compressor (1), signals are communicated with each other, and when a switch in the switch group (2) is turned on, a power supply is turned on, the programmable controller (4) is started, and an operation signal is sent;
preferably, the programmable controller (4) uses a PLC model FP-1616T/R _ M for 24v power supply 16 in and out; preferably, the propulsion controller (1) uses a model CL-01A intelligent stepping servo motor pulse generator, and the propeller uses a 42 BYGH601.5A two-phase stepping motor;
2. the operation system comprises a propeller (9), a propulsion tank (19), electric three-way ball valves (20) - (23), water pumps (5) and (11), an air pump (10) and a magnetic stirrer (6). The programmable controller (4) sends an operation signal to the propulsion controller or the air compressor (1), and the propulsion controller or the air compressor (1) starts the propeller (9) to move forward or backward to drive the piston in the propulsion tank (19) to move forward or backward; when the piston retreats, the reaction liquid B in the liquid storage tank (18) is sucked into the propelling tank (19) through the electric three-way ball valve (23), and when the piston advances, the reaction liquid B in the propelling tank (19) is pushed into the reaction tank (17) through the electric three-way ball valve (23);
preferably, the volume of the propelling tank (19) is 50-150 ml;
preferably, the volume of the reaction tank (17) is 2-3L;
3. a pipeline system:
preferably, the inner diameter of the operation pipeline is generally 8mm, and the outer diameter is 12 mm; the inlet and outlet pipelines of the electric three-way ball valve (23) have the inner diameter of 6mm and the outer diameter of 10 mm; the water pump (11) adopts a peristaltic pump, and the inner diameter of a pipeline of the peristaltic pump is 1mm, and the outer diameter of the pipeline is 3 mm;
4. the programmable controller (4) sends a signal to adjust the steering of ball valves (20) - (22) in the operation system, simultaneously sends an operation signal to the relay (3) to start the water pump (5), pumps a certain amount of reaction liquid A in the liquid storage tank (14) into the reaction tank (17), and starts the magnetic stirrer (6);
preferably, the ball valves (20) - (23) adopt a three-way valve controlled by 24v direct current and 3 wires, the power of the water pumps (5), (11) is 100w, and the magnetic stirrer (6) adopts a strong magnetic stirrer;
5. preparing a bubble stone: starting a water pump (11) by using a programmable controller (4), humidifying the air bubble stone (7) for 10-30 s, and then starting an air pump (10) to blow air to the air bubble stone (7);
preferably, the water pump (11) uses a peristaltic pump, and the flow rate is 5 ml/min; preferably, the bubbled stone is a nano bubbled stone with the diameter of 4 cm;
6. and (3) microcapsule generation: the programmable controller (4) adjusts the rotation direction of the electric three-way ball valve (23) according to a feedback signal of the propulsion controller or the air compressor (1), then sends a signal to the propulsion controller or the air compressor (1), the propulsion controller or the air compressor (1) immediately adjusts the propeller (9) to push the piston in the propulsion tank (19) to advance, so that the reaction liquid B is dripped into a slit of the bubble stone (7) through the electric three-way ball valve (23) and the dripper (8) to generate a bubble ball, and then is dripped into the reaction tank (17), and the reaction liquid A, B generates microcapsules in the reaction tank (17) under the continuous stirring of the magnetic stirrer (6);
7. and (3) recovering microcapsules: the programmable controller (4) adjusts the electric three-way ball valves (21-22) through the relay (3) and starts the water pump (5), so that reaction liquid in the reaction tank (17) forms circulation among the filter vat (16), the capsule storage tank (15), the electric three-way ball valve (21), the water pump (5), the electric three-way ball valve (22) and the reaction tank (17) and continuously circulates, and the capsule is intercepted in the filter vat (16);
preferably, the filter barrel (16) has a filter hole diameter of 1mm, and the capsule storage tank is a vacuum tank.
EXAMPLE 2 Sterilization of containers and lines with ozone
1) Transferring a water pipe head connected with the liquid storage tank (13) to the liquid storage tank (12), and transferring a water pipe head connected with the liquid storage tank (14) to the reaction tank (17) so as to respectively form a loop around the inlet and outlet of the liquid storage tank (12) and the inlet and outlet of the reaction tank (17) during disinfection, thereby facilitating the recycling of disinfectant;
2) filling water in the liquid storage tank (12) and introducing ozone for 30min continuously; the switch group (2) is set to be in a disinfection mode, and disinfection is started;
preferably, a FL-803A ozone machine is used, and the ozone generating capacity is 3 g/h;
3) the programmable controller (4) controls the electric three-way ball valves (20) - (22) and the water pump (5) to pump ozone water from the liquid storage tank (12) into the reaction tank (17);
4) the programmable controller (4) controls the electric three-way ball valves (20) - (22) and the water pump (5), and ozone water circulates in the loops of the liquid storage tank (12), the electric three-way ball valves (20) - (21), the water pump (5) and the electric three-way ball valve (22) for a plurality of minutes by utilizing the loops which go in and out around the liquid storage tank (12);
5) the programmable controller (4) controls the electric three-way ball valves (20) - (22) and the water pump (5) to enable ozone water to enter loops of the electric three-way ball valve (21), the water pump (5), the electric three-way ball valve (22), the reaction tank (17) and the capsule storage tank (15) from the liquid storage tank (12) through the electric three-way ball valve (20) for circulation for a plurality of minutes;
6) the programmable controller (4) controls the electric three-way ball valves (21) - (22) and the water pump (5) to ensure that ozone water enters a loop which goes in and out around the liquid storage tank (12) from the reaction tank (17) through the capsule storage tank (15) and the electric three-way ball valve (20) and is discharged into the liquid storage tank (12) for reozonation.
Example 3 preparation of calcium alginate solid capsules
The method comprises the following specific steps:
1) preparing reaction liquids A and B;
preferably, the main component of the reaction solution A is a calcium salt solution, and the main component of the reaction solution B is an alginate solution.
| name of reagent | Dosage form | |
| 1. | 10g | |
| 2. Bacterial liquid | 100ml |
2) Adding the concentrated solution of the reaction solution A into a liquid storage tank (14), and adding the reaction solution B into a liquid storage tank (18);
3) programming on a programming controller;
4) starting a switch in a switch group (2), starting a programmable controller (4) to adjust the steering directions of electric three-way ball valves (20) - (22), simultaneously starting a relay (3) to start a water pump (5), pumping 200ml of concentrated solution of reaction liquid A in a liquid storage tank (14) into a reaction tank (17), then starting the electric three-way ball valve (20) by the programmable controller (4), starting the water pump (5), taking 800ml of water from the liquid storage tank (12) to dilute the water into working concentration, and then starting a magnetic stirrer (6);
preferably, the power of the water pump (5) is 100 w;
5) preparing a bubble stone: starting a water pump (11) by using a programmable controller (4), humidifying the air bubble stone (7) for 10-30 s, and then starting an air pump (10) to blow air to the air bubble stone (7);
6) preparing microcapsules: the programmable controller (4) adjusts the rotation direction of the electric three-way ball valve (23) according to a feedback signal of the propulsion controller or the air compressor (1), then sends a signal to the propulsion controller or the air compressor (1), the propulsion controller or the air compressor (1) immediately adjusts the propeller (9) to push the piston in the propulsion tank (19) to advance, so that the reaction liquid B is dripped into a slit of the bubble stone (7) through the electric three-way ball valve (23) and the dripper (8) to generate a bubble ball, and then is dripped into the reaction tank (17) to react with the reaction liquid A under the stirring of the magnetic stirrer (6) to form a solid capsule, after the propulsion is finished, the reaction is continued for a plurality of minutes under the magnetic stirring as required, so that the reaction is completely finished;
7) and (3) recovering microcapsules: the programmable controller (4) adjusts the electric three-way ball valves (21) - (22) and starts the water pump (5), so that liquid in the reaction tank (17) forms circulation among the filter vat (16), the capsule storage tank (15), the electric three-way ball valve (21), the water pump (5), the electric three-way ball valve (22) and the reaction tank (17), and finally generated capsules are intercepted in the filter vat (16);
8) microcapsule cleaning: the programmable controller (4) adjusts the electric three-way ball valves (20) - (22), starts the water pump (5), pumps the cleaning agent in the liquid storage tank (12) into the reaction tank (17), and repeats the step 7) to clean the capsule; then the programmable controller (4) adjusts the electric three-way ball valve (22), turns on the water pump (5), and discharges the liquid as waste liquid into the liquid storage tank (13);
9) and (3) automatically and repeatedly executing: according to the preset repetition times, the programmable controller (4) automatically repeats the steps 4) -8); after the repetition times are finished, opening a capsule storage tank (15), and taking out capsules in a filter barrel (16);
generally 1L of the reaction solution B can prepare capsules with the volume of nearly 1L;
10) the switch group is provided with the buttons of the steps, if all the buttons are pressed, the steps can be automatically completed at one time, and individual buttons can be independently pressed to complete a plurality of the steps for debugging the capsule.
Claims (9)
1. An automatic device for producing a bubble capsule microbial inoculum is characterized in that a propulsion controller or an air compressor (1), a switch group (2), a relay (3) and a programmable logic controller (4) are utilized to control a propeller or a dispenser (9), electric three-way ball valves (20) - (23), a water pump (5), a magnetic stirrer (6) and a gas sepiolite (7), so that a reaction liquid A and a reaction liquid B flow among a propulsion tank or a dispensing valve (19), liquid storage tanks (12) - (14) and (18), microcapsules with micro bubbles are generated in a reaction tank (17) through reaction, and are stored in a capsule storage tank (15).
2. An automated apparatus for producing bubble encapsulating agents as claimed in claim 1, wherein a series of buttons in the switch block (2) provide various input signals to the programmable controller (4) to select and change the operation mode of the apparatus, so that the apparatus has a plurality of functions including sterilization, pause, different reagent concentration ratios, repeat number setting, and switching between the propulsion mode and the dispenser mode of the propulsion tank.
3. The automation equipment for producing bubble capsule bacterial agent as claimed in claim 1, wherein the programmable controller (4) can directly control the propulsion controller (1) with the same voltage, and can also control the electric three-way ball valves (20) - (23), the water pump (5) and the magnetic stirrer (6) with any voltage through the relay (3).
4. The automatic equipment for producing the bubble capsule bacterial agent of claim 1, wherein when the said (9) is a propeller, (19) is a propelling tank, when in use, the propelling tank is matched with an electric three-way ball valve to switch the direction, when the propeller (9) is retreated, the propelling tank can suck the reaction liquid A from the liquid storage tank (18) through the electric three-way ball valve (23), and when the propeller (9) is advanced, the propelling tank can make the liquid drop out from the dripper (8) through the direction change of the electric three-way ball valve (23); when the glue dispenser is used as the (9), the glue dispenser valve is matched with the (19), only direct dripping is needed during use, and the steering is controlled without matching with an electric three-way ball valve.
5. An automated apparatus for producing bubble encapsulating bacterial agents according to claim 1, wherein said capsule reservoir (15) is a sealed tank which can be evacuated and contains a filter cartridge (16) for collecting capsules, one end of a water inlet pipe is aligned with the bottom area in the filter cartridge (16), and the other end is connected with the reaction tank (17); one end of a water outlet pipe is positioned at the inner bottom of the capsule storage tank (15) outside the filter cartridge (16), and the other end is connected with an electric three-way ball valve (21).
6. The automatic equipment for producing bubble capsule fungicide according to claim 1, wherein the electromagnetic stirrer (6) provides stirring power to the bottom of the reaction tank (17), the dripper (8) for dripping the reaction solution is arranged above the reaction tank, the bubbled stone (7) is arranged between the dripper (8) and the reaction tank, and the reaction solution dripped by the dripper generates a large amount of small bubbles when passing through the slit of the bubbled stone and then enters the reaction tank (17) to generate bubble capsules while stirring.
7. The automatic equipment for producing the bubble capsule fungicide according to claim 1, wherein the air stone (7) is composed of two air stones facing each other, a slit with a thickness of 1-4mm is formed in the middle, the air ventilation of the air stones is controlled by an air pump (10), and a water pump (11) independently sucks a small amount of water to add liquid and preserve moisture to the air sepiolite.
8. A sterilization method for the automatic apparatus for producing bubble encapsulating microbial inoculum according to claim 1, characterized in that the method comprises the following steps:
1) transferring a water pipe head connected with the liquid storage tank (13) to the liquid storage tank (12), and transferring a water pipe head connected with the liquid storage tank (14) to the reaction tank (17) so as to form loops (12) - (13) and (14) - (17) during disinfection, thereby facilitating the recycling of disinfectant;
2) water is filled in the liquid storage tank (12) and ozone is introduced, the switch group (2) is set to be in a disinfection mode, and disinfection is started;
3) the programmable controller (4) controls the electric three-way ball valves (20) - (22) and the water pump (5) to pump ozone water from the liquid storage tank (12) into the reaction tank (17);
4) the programmable controller (4) controls the electric three-way ball valves (20) - (22) and the water pump (5), and the loops (14) - (17) are utilized to enable ozone water to circulate for a plurality of minutes in the loops of the reaction tank (17), the electric three-way ball valve (20), the electric three-way ball valve (21), the water pump (5) and the electric three-way ball valve (22);
5) the programmable controller (4) controls the electric three-way ball valves (21) - (22) and the water pump (5) to enable ozone water to circulate in loops of the reaction tank (17), the capsule storage tank (15), the electric three-way ball valve (21), the water pump (5) and the electric three-way ball valve (22) for a plurality of minutes;
6) the programmable controller (4) controls the electric three-way ball valves (21) - (22) and the water pump (5) to discharge ozone water from the reaction tank (17) to the liquid storage tank (12) for reozonation through the capsule storage tank (15), the electric three-way ball valve (21) and the electric three-way ball valve (22) by utilizing loops of (12) - (13);
7) repeating the steps 3) -6) for a plurality of times to complete the disinfection of the pipelines and each liquid storage tank, and restoring each pipeline to the original position.
9. A production method for the automated apparatus for producing bubble encapsulating microbial inoculum according to claim 1, characterized in that the method comprises the following steps:
1) preparing a reaction solution A (the content of parentheses in the claims cannot be included) and a reaction solution B, wherein the main component of the reaction solution A is a calcium salt solution, and the main component of the reaction solution B is an alginate solution;
2) the programmable controller (4) adjusts the steering of the ball valves (20) - (22), the water pump (5) is started, a certain amount of reaction liquid A in the liquid storage tank (14) is pumped into the reaction tank (17), and the magnetic stirrer (6) is started; the programmable controller (4) starts the propulsion controller (1) to retreat the propeller (9) so that the propulsion tank (19) sucks the reaction liquid B from the liquid storage tank (18); if the propeller or the dispenser (9) is a dispenser, the subsequent dripping step is directly executed without back preparation;
3) preparing a bubble stone: starting a water pump (11) by using a programmable controller (4), humidifying the air bubble stone (7) for 10-30 s, and then starting an air pump (10) to blow air to the air bubble stone (7);
4) preparing microcapsules: the programmable controller (4) adjusts the rotation direction of the electric three-way ball valve (23) according to a feedback signal of the propulsion controller (1), then sends a signal to the propulsion controller (1), the propulsion controller (1) immediately adjusts the propeller (9) to push the propulsion tank (19), so that the reaction liquid B is dripped into a slit of the air bubble stone (7) through the electric three-way ball valve (23) and the dripper (8) to generate an air bubble ball, and then enters the reaction tank (17) to react with the reaction liquid A under the stirring of the magnetic stirrer (6) to form a solid capsule, after the propulsion is finished, the reaction liquid B is stirred for a plurality of minutes under the magnetic stirring as required, and the reaction is fully finished;
5) and (3) recovering microcapsules: the programmable controller (4) adjusts the electric three-way ball valves (21) - (22) and starts the water pump (5) to enable the liquid in the reaction tank (17) to form circulation among the capsule storage tank (15), the electric three-way ball valve (21), the water pump (5) and the electric three-way ball valve (22), and finally the capsule is trapped in the filter vat (16);
6) microcapsule cleaning: the programmable controller (4) adjusts the electric three-way ball valves (20) - (22), the water pump (5) is started, cleaning liquid in the liquid storage tank (12) is pumped into the reaction tank (17), the step (3) is repeated to clean the capsules, after the cleaning is finished, the programmable controller (4) adjusts the electric three-way ball valves (21) - (22), the water pump (5) is started, and the liquid is discharged into the liquid storage tank (13) as waste liquid;
7) automatically and repeatedly executing; and (3) automatically repeating the steps (1) to (6) according to the set repetition times, finally opening the capsule storage tank (15), and taking out the capsules in the filter barrel (16).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110067432.4A CN112808187B (en) | 2021-01-19 | 2021-01-19 | Automatic equipment and method for producing bubble capsule microbial inoculum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110067432.4A CN112808187B (en) | 2021-01-19 | 2021-01-19 | Automatic equipment and method for producing bubble capsule microbial inoculum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112808187A true CN112808187A (en) | 2021-05-18 |
| CN112808187B CN112808187B (en) | 2023-05-30 |
Family
ID=75870041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110067432.4A Active CN112808187B (en) | 2021-01-19 | 2021-01-19 | Automatic equipment and method for producing bubble capsule microbial inoculum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112808187B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114951100A (en) * | 2022-05-19 | 2022-08-30 | 江苏泰芯源科技有限公司 | Environment-friendly cleaning equipment for processing electronic components and use method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007021315A (en) * | 2005-07-13 | 2007-02-01 | National Institute Of Advanced Industrial & Technology | Method for producing hollow microcapsule |
| JP2011078911A (en) * | 2009-10-07 | 2011-04-21 | Kagoshima Univ | Microcapsule encapsulating denitrifying bacteria and production method thereof |
| TW201217046A (en) * | 2010-10-29 | 2012-05-01 | bo-hong Liu | ensures the capsules to show a preset circle shape through the cooling titration process so as to greatly improve its process quality |
| CN103125427A (en) * | 2011-12-02 | 2013-06-05 | 吕爱忠 | Fish pond oxygen filling pump with air energy thermal drive |
| CN104519989A (en) * | 2012-06-26 | 2015-04-15 | 通用电气医疗集团股份有限公司 | Preparation of composition comprising gas microbubbles |
| CN107522293A (en) * | 2017-10-16 | 2017-12-29 | 郑州大学 | It is a kind of based on denitrification organisms microcapsules and the ecological purified water preparation method of granules of slow release carbon source |
-
2021
- 2021-01-19 CN CN202110067432.4A patent/CN112808187B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007021315A (en) * | 2005-07-13 | 2007-02-01 | National Institute Of Advanced Industrial & Technology | Method for producing hollow microcapsule |
| JP2011078911A (en) * | 2009-10-07 | 2011-04-21 | Kagoshima Univ | Microcapsule encapsulating denitrifying bacteria and production method thereof |
| TW201217046A (en) * | 2010-10-29 | 2012-05-01 | bo-hong Liu | ensures the capsules to show a preset circle shape through the cooling titration process so as to greatly improve its process quality |
| CN103125427A (en) * | 2011-12-02 | 2013-06-05 | 吕爱忠 | Fish pond oxygen filling pump with air energy thermal drive |
| CN104519989A (en) * | 2012-06-26 | 2015-04-15 | 通用电气医疗集团股份有限公司 | Preparation of composition comprising gas microbubbles |
| CN107522293A (en) * | 2017-10-16 | 2017-12-29 | 郑州大学 | It is a kind of based on denitrification organisms microcapsules and the ecological purified water preparation method of granules of slow release carbon source |
Non-Patent Citations (1)
| Title |
|---|
| 张俊 等: "《大孔型NaCS-PDMDAAC微胶囊固定化细胞在摇床和鼓泡塔中的培养研究》", 《生物工程学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114951100A (en) * | 2022-05-19 | 2022-08-30 | 江苏泰芯源科技有限公司 | Environment-friendly cleaning equipment for processing electronic components and use method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112808187B (en) | 2023-05-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112808187B (en) | Automatic equipment and method for producing bubble capsule microbial inoculum | |
| CN208038342U (en) | A kind of organic fertilizer and the automatic weighing proportioning device of inorganic fertilizer | |
| CN102630629A (en) | Aerating device of water body and aeration filtering device of water body | |
| CN112403410B (en) | Multipurpose microcapsule preparation device and method | |
| CN114672407A (en) | Anti-pollution formula bacteria culture device of clinical laboratory | |
| CN204275824U (en) | Three groove continous way drug delivery dissolvers | |
| CN204469565U (en) | A kind of water treatment system device for continuously adding medicine | |
| CN214142123U (en) | Preparation device for preparing high-amino-acid liquid fertilizer by utilizing yellow serofluid | |
| CN208517295U (en) | A kind of organic fertilizer production equipment | |
| CN101618309B (en) | Automatic solid water production line and method for preparing solid water | |
| CN202983535U (en) | Three-groove continuous automatic dosing dissolving device | |
| CN219376990U (en) | High-cost-performance nutrient solution automatic control stirring equipment and plant planting system | |
| CN114950230A (en) | Organic fertilizer proportioning and adding device for culture medium and using method thereof | |
| CN102257898A (en) | Rotary type plant fruit soaking, sprouting and growing device | |
| CN215517396U (en) | Fermenting installation for microbial fermentation shrimp shell | |
| CN206314429U (en) | A kind of multi-functional fish pond aerator | |
| CN218321373U (en) | Microalgae culture reactor | |
| CN218321372U (en) | Microalgae culture reactor | |
| CN215196442U (en) | Compound yicotai plant carbon source preparation system taking imported stock solution as pioneer raw material | |
| CN214320154U (en) | Dissolving device and system for oxygen-enriched dissolved copper | |
| CN104190277A (en) | Three-tank continuous drug-delivering dissolving system | |
| CN212051442U (en) | Culture apparatus is used in fertile production of microorganism | |
| CN217887799U (en) | Automatic mend fertile system of joining in marriage of accurate joining in marriage of mother liquor | |
| CN221832347U (en) | Reaction kettle for preparing phloroglucinol | |
| CN214571371U (en) | Aerobic digestion sludge pool device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |