CN107522293A - It is a kind of based on denitrification organisms microcapsules and the ecological purified water preparation method of granules of slow release carbon source - Google Patents

It is a kind of based on denitrification organisms microcapsules and the ecological purified water preparation method of granules of slow release carbon source Download PDF

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CN107522293A
CN107522293A CN201710960257.5A CN201710960257A CN107522293A CN 107522293 A CN107522293 A CN 107522293A CN 201710960257 A CN201710960257 A CN 201710960257A CN 107522293 A CN107522293 A CN 107522293A
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purified water
microcapsules
sodium alginate
chitosan
solution
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CN107522293B (en
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于鲁冀
程璐璐
刘露
李廷梅
范鹏宇
刘攀龙
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Zhengzhou University
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Zhengzhou University
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/28—Treatment of water, waste water, or sewage by sorption
    • C02F1/281—Treatment of water, waste water, or sewage by sorption using inorganic sorbents
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/34—Organic compounds containing oxygen
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/38—Organic compounds containing nitrogen

Abstract

The present invention relates to based on the ecological purified water preparation method of granules of denitrification organisms microcapsules and slow release carbon source, effectively solve micropollutant water denitrification microorganism deficiency, C/N is low, biodegradability is poor, be not easily recycled and the problem of secondary pollution.Microorganism fungus kind is cultivated, into denitrification microorganism；Chitosan is dissolved in acetic acid solution, into chitosan solution；Sodium alginate is soluble in water, sorbester p17 is added, into sodium alginate soln；Sodium alginate soln and denitrification microorganism are mixed, instill CaCl2Solution, into calcium alginate gel, calcium alginate gel is put into secondary solidification in chitosan solution, into microcapsules；Microcapsules are put into sodium citrate solution, intermediate layer calcium alginate liquefaction are carried out, into bio-microcapsule；Hydrophilic material, pore-creating character material, binder, slow release carbon source, bio-microcapsule are well mixed, spherical ecological purified water particle is made, is conserved,.Material of the present invention is abundant, cheap, microorganism nonhazardous is acted on, recyclable, non-secondary pollution.

Description

It is a kind of based on denitrification organisms microcapsules and the ecological purified water particle system of slow release carbon source Preparation Method
Technical field
It is particularly a kind of based on denitrification organisms microcapsules and the ecological purified water of slow release carbon source the present invention relates to chemical industry Grain preparation method.
Background technology
Water body polluted by nitrogen is one of important environmental problem, and river, lake and the underground water of China various regions are different degrees of On by polluted by nitrogen, degraded and removal to nitrogen are the important goals of polluted-water restoration of the ecosystem.The removal of Water is main Realized by denitrification microorganism, and China it is common be all low C/N sewage, biodegradability is poor, limits the conversion and removal of nitrogen, Therefore providing a number of denitrification microorganism and supplementary carbon source turns into one of effective ways for solving water body polluted by nitrogen.
At present, in order to improve denitrification microorganism quantity, frequently with method be that denitrification microorganism is directly added into water, but Natural water environment is complicated and changeable, and it is low and be difficult to turn into dominant bacteria directly to add denitrification microorganism survival rate.Microbe microcapsules Technology is a kind of one of new process for fixation, in the 1960s, Chang reports the preparation of pellicle microcapsules, it is indicated that Bioactive substance and the cells such as protein, enzyme are embedded with it, biological substance can be kept active；In the early 1980s, Lim and Sun is successfully prepared sodium alginate-polylysine microcapsules.Denitrification microorganism is passed through by certain technology using microcapsules technology Means are coated in the film of high polymer material formation, and good living environment is provided for the growth and breeding of denitrification microorganism.Research In commonly use carbon source such as methanol, ethanol, glucose etc., it is preferable for carbon effect, but exist cost and operating cost it is higher and launch Amount is difficult to control, easily causes secondary pollution problems.In recent years, the agricultural wastes rich in a large amount of celluloses replace traditional carbon source It has been be used widely that, this kind of material is cheap, and is readily biodegradable, and has a good application prospect.But how Denitrification microorganism Mendoza Pseudomonas alba will be coated（Pseudomonas mendocina）, Pseudomonas putidas （Pseudomonas putida）, heterotrophic nitrification-aerobic denitrification bacterium（Paracoccus pantotrophus）Microcapsules and Slow release carbon source corncob, peanut shell etc. are combined together and life are made by some hydrophilic materials, pore-creating character material and binder State water purification particle, to solve in polluted by nitrogen removal process the problems such as denitrification microorganism deficiency is low with C/N, meanwhile, ecological purified water Particle is a diameter of 28 ~ 32mm spheric granules, has certain suction-operated to pollutant, using being easy to reclaim after terminating, is kept away Exempt to cause the secondary pollution to river course, so far there are no is publicly reported.
The content of the invention
For the above situation, to overcome the defect of prior art, the purpose of the present invention is just to provide one kind with denitrification organisms Microcapsules and the ecological purified water preparation method of granules that slow release carbon source is main body, can effectively solve micropollutant water denitrification microorganism not Foot, C/N is low, biodegradability is poor, the problem of being not easily recycled and easily cause secondary pollution.
The technical scheme that the present invention solves is, first using emulsion-crosslinking method and polyelectrolyte coacervation by denitrification microorganism It is coated on using high polymer material in the microcapsules of outer wall, then to carry out the preparation of ecological purified water particle；The addition of slow release carbon source Be advantageous to improve the biodegradability of river, improve nitric efficiency；Ecological purified water particle has using inorganic material as skeleton to pollutant There is certain suction-operated, specifically include following steps：
（1）, prepare denitrification microorganism
Microorganism fungus kind is accessed in liquid medium within, inoculum concentration is the 1% ~ 3% of fluid nutrient medium weight, in 30 ± 1 DEG C, rotating speed Under the conditions of 150r/min, concussion and cultivate 36h, into denitrification microorganism；
Described fluid nutrient medium is (NH4)2SO4 0.5g、C4H4Na2O4 5.2g、K2HPO4 0.25g、MgSO4·7H2O 0.125g、NaCl 0.125g、FeSO4·7H2O 0.0025g、MnSO4·4H2The g of O 0.0025, supplement distilled water is extremely 1000mL, regulation pH to 7.0 ~ 7.2 are made；
The microorganism fungus kind is Mendoza Pseudomonas alba（Pseudomonas mendocina）, Pseudomonas putidas （Pseudomonas putida）, heterotrophic nitrification-aerobic denitrification bacterium（Paracoccus pantotrophus）One kind or two The mixing of the weight such as kind；
（2）, prepare microcapsules
Prepare chitosan solution：Chitosan is dissolved in the acetic acid solution of mass concentration 1% in 60 DEG C of constant temperature stirrings, into chitosan Solution, chitosan mass concentration are 0.5% ~ 2%；
Prepare sodium alginate soln：90 DEG C of constant temperature stirrings of sodium alginate are soluble in water, and it is 2 ~ 3% to be prepared into mass concentration Sodium alginate soln, then emulsifier span 80 is added in sodium alginate soln（span80）, sorbester p17 mass concentration be 0.2 ~ 0.5%；
Sodium alginate soln is well mixed with denitrification microorganism with weight than 1 ︰ 1, is placed in pellet fabrication device, instills mass concentration 4% CaCl2Solution, stirred when being added dropwise, mixing speed is 200 ~ 300 r/min, mixing time 40min, into sodium alginate The calcium alginate gel of parcel, calcium alginate gel is collected, it is clean with aseptic water washing, cross 100-150 mesh sieves；
The calcium alginate gel for crossing 100-150 mesh sieves is put into chitosan solution, carries out secondary solidification 10 ~ 15min, Cheng Wei Capsule, with aseptic water washing 2 ~ 3 times；
The microcapsules that aseptic water washing is crossed are put into the sodium citrate solution that concentration is 0.06mol/L ~ 0.10mol/L, are entered The liquefaction of row intermediate layer calcium alginate, room temperature liquefaction 7-9 min；
The microcapsules after liquefaction are collected, are cleaned three times with ultra-pure water, vacuum freeze drying 24h ~ 36h, into bio-microcapsule；
（3）, prepare ecological purified water particle
Ecological purified water particle is by percentage by weight meter：Hydrophilic material 45-55%, pore-creating character material 22-26%, binder 9-11%, slow release carbon source 5-7% and bio-microcapsule 8-12% are made up of raw material, wherein, by hydrophilic material, pore-creating character material, stick Mixture, slow release carbon source, bio-microcapsule are well mixed, and are inserted in granulating disc, under 25 ~ 30r/min rotating speeds, use spraying Hydraulic giant constantly sprays water smoke into comminutor, raw material is constantly rolled into 28 ~ 32 mm spherical ecological purified water particle；
Described hydrophilic material is the mixing of one or both of flyash, diatomite, bentonite；
Described pore-creating character material is zeolite powder or vermiculite power；
Described binder is portland cement；
Described slow release carbon source is the mixing of one or both of corncob or peanut shell；
Maintenance：Ecological purified water particle is placed in incubator, continuously conserves 7d, curing temperature is 30 DEG C, humidity 85% Rh, as finished product.
Material of the present invention is abundant, it is cheap, to microorganism nonhazardous effect, secondary pollution will not be caused, be easy to reclaim, It is the new breakthrough of biological prosthetic micropollutant water, first using emulsion-crosslinking method and polyelectrolyte coacervation by denitrification microorganism bag Overlay on using high polymer material in the microcapsules of outer wall, then to carry out the preparation of ecological purified water particle；Slow release carbon source is added with Beneficial to the biodegradability for improving river, nitric efficiency is improved；Ecological purified water particle has using inorganic material as skeleton to pollutant Suction-operated, economic and social benefit are notable.
Brief description of the drawings
Fig. 1 is ecological purified water particle internal structure schematic diagram of the present invention.
Fig. 2 is removal effect figure of the ecological purified water particle of the present invention to simulated wastewater.
Fig. 3 is toxic Test Drawing of the ecological purified water particle of the present invention to microorganism.
Fig. 4 is degradation curve figure of the ecological purified water particle of the present invention in water.
Embodiment
The embodiment of the present invention is elaborated below in conjunction with concrete condition.
Embodiment 1
The present invention specifically includes following steps in specific implementation：
（1）, prepare denitrification microorganism
Mendoza Pseudomonas alba is accessed in liquid medium within（Pseudomonas mendocina）, Pseudomonas putidas （Pseudomonas putida）, Mendoza Pseudomonas alba inoculum concentration is the 1% of fluid nutrient medium weight, Pseudomonas putida bar Bacterium inoculum concentration is the 1% of fluid nutrient medium weight, under the conditions of 30 ± 1 DEG C, rotating speed are 150r/min, concussion and cultivate 36h, into de- Nitrogen microorganism；
（2）, prepare microcapsules
Prepare chitosan solution：Chitosan is dissolved in the acetic acid solution of mass concentration 1% in 60 DEG C of constant temperature stirrings, into chitosan Solution, chitosan mass concentration are 0.5%；
Prepare sodium alginate soln：90 DEG C of constant temperature stirrings of sodium alginate are soluble in water, it is prepared into the sea that mass concentration is 2% Solution of sodium alginate, then emulsifier span 80 is added in sodium alginate soln（span80）, sorbester p17 mass concentration is 0.5%；
Sodium alginate soln is well mixed with denitrification microorganism with weight than 1 ︰ 1, is placed in pellet fabrication device, instills mass concentration 4% CaCl2Solution, stir, mixing speed 200r/min, mixing time 40min, wrapped up into sodium alginate when being added dropwise Calcium alginate gel, collect calcium alginate gel, it is clean with aseptic water washing, cross 100-150 mesh sieves；
The calcium alginate gel for crossing 100-150 mesh sieves is put into chitosan solution, secondary solidification 10min is carried out, into micro- glue Capsule, with aseptic water washing 2 ~ 3 times；
The microcapsules that aseptic water washing is crossed are put into the sodium citrate solution that concentration is 0.06mol/L, carry out intermediate layer sea The liquefaction of calcium alginate, 8 min of room temperature liquefaction；
The microcapsules after liquefaction are collected, are cleaned three times with ultra-pure water, vacuum freeze drying 24h ~ 36h, into bio-microcapsule；
（3）, prepare ecological purified water particle
Ecological purified water particle is by percentage by weight meter：Flyash 20%, diatomite 30%, vermiculite power 24%, portland cement 10%th, corncob 6% and bio-microcapsule 10% are made up of raw material, wherein, by flyash, diatomite, vermiculite power, portland cement, Corncob and bio-microcapsule are well mixed, and are inserted in granulating disc, under 25 ~ 30r/min rotating speeds, using water fog nozzle to Water smoke is constantly sprayed in comminutor, raw material is constantly rolled into 28 ~ 32 mm spherical ecological purified water particle；
Maintenance：Ecological purified water particle is placed in incubator, continuously conserves 7d, curing temperature is 30 DEG C, humidity 85% Rh, as finished product.
Embodiment 2
The present invention specifically includes following steps in specific implementation：
（1）, prepare denitrification microorganism
Microorganism fungus kind heterotrophic nitrification-aerobic denitrification bacterium is accessed in liquid medium within（Paracoccus pantotrophus）, inoculum concentration is the 3% of fluid nutrient medium weight, under the conditions of 30 ± 1 DEG C, rotating speed are 150r/min, concussion 36h is cultivated, into denitrification microorganism；
（2）, prepare microcapsules
Prepare chitosan solution：Chitosan is dissolved in the acetic acid solution of mass concentration 1% in 60 DEG C of constant temperature stirrings, into chitosan Solution, chitosan mass concentration are 1%；
Prepare sodium alginate soln：90 DEG C of constant temperature stirrings of sodium alginate are soluble in water, and it is 2.5% to be prepared into mass concentration Sodium alginate soln, then emulsifier span 80 is added in sodium alginate soln（span80）, sorbester p17 mass concentration is 0.5%；
Sodium alginate soln is well mixed with denitrification microorganism with weight than 1 ︰ 1, is placed in pellet fabrication device, instills mass concentration 4% CaCl2Solution, stirred when being added dropwise, mixing speed is 250 r/min, mixing time 40min, is wrapped up into sodium alginate Calcium alginate gel, collect calcium alginate gel, it is clean with aseptic water washing, cross 100-150 mesh sieves；
The calcium alginate gel for crossing 100-150 mesh sieves is put into chitosan solution, secondary solidification 12min is carried out, into micro- glue Capsule, with aseptic water washing 2 ~ 3 times；
The microcapsules that aseptic water washing is crossed are put into the sodium citrate solution that concentration is 0.08mol/L, carry out intermediate layer sea The liquefaction of calcium alginate, room temperature liquefaction 9min；
The microcapsules after liquefaction are collected, are cleaned three times with ultra-pure water, vacuum freeze drying 24h ~ 36h, into bio-microcapsule；
（3）, prepare ecological purified water particle
Ecological purified water particle is by percentage by weight meter：Bentonite 20%, diatomite 30%, zeolite powder 24%, portland cement 10%th, peanut shell 6% and bio-microcapsule 10% are made up of raw material, wherein, by hydrophilic material, pore-creating character material, binder, slow Release carbon source, bio-microcapsule is well mixed, insert in granulating disc, under 25 ~ 30r/min rotating speeds, using water fog nozzle to Water smoke is constantly sprayed in comminutor, raw material is constantly rolled into 28 ~ 32 mm spherical ecological purified water particle；
Maintenance：Ecological purified water particle is placed in incubator, continuously conserves 7d, curing temperature is 30 DEG C, humidity 85% Rh, as finished product.
Embodiment 3
The present invention specifically includes following steps in specific implementation：
（1）, prepare denitrification microorganism
Cut-in quality is than the microorganism fungus kind Mendoza Pseudomonas albas of 1 ︰ 1 in liquid medium within（Pseudomonas mendocina）, heterotrophic nitrification-aerobic denitrification bacterium（Paracoccus pantotrophus）, inoculum concentration is fluid nutrient medium The 2.5% of weight, under the conditions of 30 ± 1 DEG C, rotating speed are 150r/min, concussion and cultivate 36h, into denitrification microorganism；
（2）, prepare microcapsules
Prepare chitosan solution：Chitosan is dissolved in the acetic acid solution of mass concentration 1% in 60 DEG C of constant temperature stirrings, into chitosan Solution, chitosan mass concentration are 1%；
Prepare sodium alginate soln：90 DEG C of constant temperature stirrings of sodium alginate are soluble in water, and it is 2 ~ 3% to be prepared into mass concentration Sodium alginate soln, then emulsifier span 80 is added in sodium alginate soln（span80）, sorbester p17 mass concentration is 0.4%；
Sodium alginate soln is well mixed with denitrification microorganism with weight than 1 ︰ 1, is placed in pellet fabrication device, instills mass concentration 4% CaCl2Solution, stir, mixing speed 300r/min, mixing time 40min, wrapped up into sodium alginate when being added dropwise Calcium alginate gel, collect calcium alginate gel, it is clean with aseptic water washing, cross 100-150 mesh sieves；
The calcium alginate gel for crossing 100-150 mesh sieves is put into chitosan solution, secondary solidification 14min is carried out, into micro- glue Capsule, with aseptic water washing 2 ~ 3 times；
The microcapsules that aseptic water washing is crossed are put into the sodium citrate solution that concentration is 0.10mol/L, carry out intermediate layer sea The liquefaction of calcium alginate, room temperature liquefaction 7.5min；
The microcapsules after liquefaction are collected, are cleaned three times with ultra-pure water, vacuum freeze drying 24h ~ 36h, into bio-microcapsule；
（3）, prepare ecological purified water particle
Ecological purified water particle is by percentage by weight meter：Flyash 22%, bentonite 30%, vermiculite power 25%, portland cement 9%th, corncob 2%, peanut shell 3% and bio-microcapsule 9% are made up of raw material, wherein, by hydrophilic material, pore-creating character material, stick Mixture, slow release carbon source, bio-microcapsule are well mixed, and are inserted in granulating disc, under 25 ~ 30r/min rotating speeds, use spraying Hydraulic giant constantly sprays water smoke into comminutor, raw material is constantly rolled into 28 ~ 32 mm spherical ecological purified water particle；
Maintenance：Ecological purified water particle is placed in incubator, continuously conserves 7d, curing temperature is 30 DEG C, humidity 85% Rh, as finished product.
Ecological purified water particle prepared by the present invention achieves very satisfied advantageous effects, had through testing and testing It is as follows to close testing data：
Removal effect of the ecological purified water particle of the present invention to simulated wastewater：
Ecological purified water particle after activation adds ratio as 8%, and simulated wastewater total nitrogen initial concentration is 20mg/L, continuous monitoring 24h, as shown in Figure 2, the nitrogen removal rate of free bacterium is 87.6%, and ecological purified water particle nitrogen removal rate of the present invention is 98.2%。
1st, ecological purified water particle of the present invention detects to the toxic of microorganism
Weigh sodium alginate 1g, chitosan 4g and sterilized under ultraviolet light and the conical flask equipped with 200mL sterilized waters is added after 24h It is interior, according to 2% access denitrification microorganism（107~108Individual/mL）, the then isothermal vibration culture under the conditions of 30 DEG C, 150r/min, Time is that 0h, 0.5h, 1h, 2h, 4h, 8h, 12h, 18h, 24h sampling carry out plate streaking, and plate count is carried out after 48h.
Sample dilution gradient is 10-1~10-8, after flat board culture clump count between 30 ~ 300 for valid data. Selection dilution 10-8Detection, as a result as shown in Figure 3：The preceding 4h contacted in high polymer material with denitrification microorganism, clump count decline compared with Substantially, microbial activity is relatively stable after 4h.Good biocompatibility can be reflected by colony growth situation.
2nd, the mass transfer performances detection of ecological purified water particle of the present invention
The certain density methylene blue solutions of 100mL are separately added into conical flask, are put into ecological purified water particle 5g, control group is not Ecological purified water particle is added, its absorbance is determined under 665nm wavelength after 24 h are shaken under the conditions of 30 DEG C, 150 r/min, is tied Fruit is as shown in table 1：Ecological purified water particle is added, 57.31% is reached to the adsorption efficiency of methylene blue, the adsorption effect having had.
The mass transfer performances of the ecological purified water particle of table 1
Absorbance Adsorption efficiency
Initially 0.998 /
Control group 0.986 2.40%
Experimental group 0.426 57.31%
3rd, the degradation curve of ecological purified water particle
Ecological purified water particle is placed in physiological saline, preserved in 4 DEG C of refrigerators.Take out at regular intervals and processing is dried After weigh its quality, experiment is carried out continuously 42d, as a result as shown in Figure 4：As can be seen that ecological purified water granular mass amplitude of variation Very small, degradation rate is slow, and it is good using stabilization, effect to show.
Indicated above, the present invention, which is combined microcapsules and inorganic material, organic carbon source etc., prepares ecological purified water matrix, Particle diameter is easy to reclaim after 28 ~ 32 mm, use, reduces environmental pollution,
The present invention compared with prior art, has advantages below：
The present invention uses natural macromolecular material sodium alginate and chitosan, and for microcapsule wall material, wide material sources are cheap, Good biocompatibility.
Slow release carbon source corncob, peanut shell etc. replace traditional carbon source such as methanol, ethanol, glucose etc., reduce cost, Cost can reduce by more than 50%, be more beneficial for engineer applied.
Microcapsules are prepared using two-step method, microcapsule membrane includes chitosan outer membrane and calcium alginate kernel；Calcium alginate Kernel is liquefied using sodium citrate, and liquid environment is provided for denitrification microorganism, is advantageous to its growth and breeding, and particle diameter is small, Specific surface area is big, and the mass transfer performances of material are high, are advantageous to the removal of pollutant.
Microcapsules of the present invention are combined with inorganic material prepares ecological purified water grain diameter 28-32mm, is easy to engineer applied With recovery, secondary pollution is avoided, energy-conserving and environment-protective, economic and social benefit is notable.

Claims (4)

  1. It is 1. a kind of based on denitrification organisms microcapsules and the ecological purified water preparation method of granules of slow release carbon source, it is characterised in that Use emulsion-crosslinking method and polyelectrolyte coacervation that denitrification microorganism is coated on to micro- glue using high polymer material as outer wall first It is intracapsular, then carry out the preparation of ecological purified water particle；Slow release carbon source is added with the biodegradability for being beneficial to improve river, improves de- Nitrogen efficiency；Ecological purified water particle has certain suction-operated to pollutant, comprised the following steps using inorganic material as skeleton：
    （1）, prepare denitrification microorganism
    Microorganism fungus kind is accessed in liquid medium within, inoculum concentration is the 1% ~ 3% of fluid nutrient medium weight, in 30 ± 1 DEG C, rotating speed Under the conditions of 150r/min, concussion and cultivate 36h, into denitrification microorganism；
    Described fluid nutrient medium is (NH4)2SO4 0.5g、C4H4Na2O4 5.2g、K2HPO4 0.25g、MgSO4·7H2O 0.125g、NaCl 0.125g、FeSO4·7H2O 0.0025g、MnSO4·4H2The g of O 0.0025, supplement distilled water is extremely 1000mL, regulation pH to 7.0 ~ 7.2 are made；
    The microorganism fungus kind is Mendoza Pseudomonas alba, Pseudomonas putidas, the one of heterotrophic nitrification-aerobic denitrification bacterium Kind or the weight such as two kinds mixing；
    （2）, prepare microcapsules
    Prepare chitosan solution：Chitosan is dissolved in the acetic acid solution of mass concentration 1% in 60 DEG C of constant temperature stirrings, it is molten into chitosan Liquid, chitosan mass concentration are 0.5% ~ 2%；
    Prepare sodium alginate soln：90 DEG C of constant temperature stirrings of sodium alginate are soluble in water, it is prepared into the sea that mass concentration is 2 ~ 3% Solution of sodium alginate, then emulsifier span 80 is added in sodium alginate soln, sorbester p17 mass concentration is 0.2 ~ 0.5%；
    Sodium alginate soln is well mixed with denitrification microorganism with weight than 1 ︰ 1, is placed in pellet fabrication device, instills mass concentration 4% CaCl2Solution, stirred when being added dropwise, mixing speed is 200 ~ 300 r/min, mixing time 40min, into sodium alginate The calcium alginate gel of parcel, calcium alginate gel is collected, it is clean with aseptic water washing, cross 100-150 mesh sieves；
    The calcium alginate gel for crossing 100-150 mesh sieves is put into chitosan solution, carries out secondary solidification 10 ~ 15min, Cheng Wei Capsule, with aseptic water washing 2 ~ 3 times；
    The microcapsules that aseptic water washing is crossed are put into the sodium citrate solution that concentration is 0.06mol/L ~ 0.10mol/L, are carried out The liquefaction of intermediate layer calcium alginate, room temperature liquefaction 7-9 min；
    The microcapsules after liquefaction are collected, are cleaned three times with ultra-pure water, vacuum freeze drying 24h ~ 36h, into bio-microcapsule；
    （3）, prepare ecological purified water particle
    Ecological purified water particle is by percentage by weight meter：Hydrophilic material 45-55%, pore-creating character material 22-26%, binder 9-11%, slow release carbon source 5-7% and bio-microcapsule 8-12% are made up of raw material, wherein, by hydrophilic material, pore-creating character material, stick Mixture, slow release carbon source, bio-microcapsule are well mixed, and are inserted in granulating disc, under 25 ~ 30r/min rotating speeds, use spraying Hydraulic giant constantly sprays water smoke into comminutor, raw material is constantly rolled into 28 ~ 32 mm spherical ecological purified water particle；
    Described hydrophilic material is the mixing of one or both of flyash, diatomite, bentonite；
    Described pore-creating character material is zeolite powder or vermiculite power；
    Described binder is portland cement；
    Described slow release carbon source is the mixing of one or both of corncob or peanut shell；
    Maintenance：Ecological purified water particle is placed in incubator, continuously conserves 7d, curing temperature is 30 DEG C, humidity 85% Rh, as finished product.
  2. It is 2. according to claim 1 based on denitrification organisms microcapsules and the ecological purified water particle preparation side of slow release carbon source Method, it is characterised in that comprise the following steps：
    （1）, prepare denitrification microorganism
    Mendoza Pseudomonas alba, Pseudomonas putidas, Mendoza Pseudomonas alba inoculum concentration are accessed in liquid medium within For the 1% of fluid nutrient medium weight, Pseudomonas putidas inoculum concentration is the 1% of fluid nutrient medium weight, in 30 ± 1 DEG C, rotating speed Under the conditions of 150r/min, concussion and cultivate 36h, into denitrification microorganism；
    （2）, prepare microcapsules
    Prepare chitosan solution：Chitosan is dissolved in the acetic acid solution of mass concentration 1% in 60 DEG C of constant temperature stirrings, it is molten into chitosan Liquid, chitosan mass concentration are 0.5%；
    Prepare sodium alginate soln：90 DEG C of constant temperature stirrings of sodium alginate are soluble in water, it is prepared into the marine alga that mass concentration is 2% Acid sodium solution, then emulsifier span 80 is added in sodium alginate soln, sorbester p17 mass concentration is 0.5%；
    Sodium alginate soln is well mixed with denitrification microorganism with weight than 1 ︰ 1, is placed in pellet fabrication device, instills mass concentration 4% CaCl2Solution, stir, mixing speed 200r/min, mixing time 40min, wrapped up into sodium alginate when being added dropwise Calcium alginate gel, collect calcium alginate gel, it is clean with aseptic water washing, cross 100-150 mesh sieves；
    The calcium alginate gel for crossing 100-150 mesh sieves is put into chitosan solution, secondary solidification 10min is carried out, into micro- glue Capsule, with aseptic water washing 2 ~ 3 times；
    The microcapsules that aseptic water washing is crossed are put into the sodium citrate solution that concentration is 0.06mol/L, carry out intermediate layer marine alga The liquefaction of sour calcium, 8 min of room temperature liquefaction；
    The microcapsules after liquefaction are collected, are cleaned three times with ultra-pure water, vacuum freeze drying 24h ~ 36h, into bio-microcapsule；
    （3）, prepare ecological purified water particle
    Ecological purified water particle is by percentage by weight meter：Flyash 20%, diatomite 30%, vermiculite power 24%, portland cement 10%th, corncob 6% and bio-microcapsule 10% are made up of raw material, wherein, by flyash, diatomite, vermiculite power, portland cement, Corncob and bio-microcapsule are well mixed, and are inserted in granulating disc, under 25 ~ 30r/min rotating speeds, using water fog nozzle to Water smoke is constantly sprayed in comminutor, raw material is constantly rolled into 28 ~ 32 mm spherical ecological purified water particle；
    Maintenance：Ecological purified water particle is placed in incubator, continuously conserves 7d, curing temperature is 30 DEG C, humidity 85% Rh, as finished product.
  3. It is 3. according to claim 1 based on denitrification organisms microcapsules and the ecological purified water particle preparation side of slow release carbon source Method, it is characterised in that comprise the following steps：
    （1）, prepare denitrification microorganism
    Microorganism fungus kind heterotrophic nitrification-aerobic denitrification bacterium is accessed in liquid medium within, inoculum concentration is fluid nutrient medium weight 3%, 30 ± 1 DEG C, rotating speed be 150r/min under the conditions of, concussion and cultivate 36h, into denitrification microorganism；
    （2）, prepare microcapsules
    Prepare chitosan solution：Chitosan is dissolved in the acetic acid solution of mass concentration 1% in 60 DEG C of constant temperature stirrings, it is molten into chitosan Liquid, chitosan mass concentration are 1%；
    Prepare sodium alginate soln：90 DEG C of constant temperature stirrings of sodium alginate are soluble in water, it is prepared into the sea that mass concentration is 2.5% Solution of sodium alginate, then emulsifier span 80 is added in sodium alginate soln, sorbester p17 mass concentration is 0.5%；
    Sodium alginate soln is well mixed with denitrification microorganism with weight than 1 ︰ 1, is placed in pellet fabrication device, instills mass concentration 4% CaCl2Solution, stirred when being added dropwise, mixing speed is 250 r/min, mixing time 40min, is wrapped up into sodium alginate Calcium alginate gel, collect calcium alginate gel, it is clean with aseptic water washing, cross 100-150 mesh sieves；
    The calcium alginate gel for crossing 100-150 mesh sieves is put into chitosan solution, secondary solidification 12min is carried out, into micro- glue Capsule, with aseptic water washing 2 ~ 3 times；
    The microcapsules that aseptic water washing is crossed are put into the sodium citrate solution that concentration is 0.08mol/L, carry out intermediate layer marine alga The liquefaction of sour calcium, room temperature liquefaction 9min；
    The microcapsules after liquefaction are collected, are cleaned three times with ultra-pure water, vacuum freeze drying 24h ~ 36h, into bio-microcapsule；
    （3）, prepare ecological purified water particle
    Ecological purified water particle is by percentage by weight meter：Bentonite 20%, diatomite 30%, zeolite powder 24%, portland cement 10%th, peanut shell 6% and bio-microcapsule 10% are made up of raw material, wherein, by hydrophilic material, pore-creating character material, binder, slow Release carbon source, bio-microcapsule is well mixed, insert in granulating disc, under 25 ~ 30r/min rotating speeds, using water fog nozzle to Water smoke is constantly sprayed in comminutor, raw material is constantly rolled into 28 ~ 32 mm spherical ecological purified water particle；
    Maintenance：Ecological purified water particle is placed in incubator, continuously conserves 7d, curing temperature is 30 DEG C, humidity 85% Rh, as finished product.
  4. It is 4. according to claim 1 based on denitrification organisms microcapsules and the ecological purified water particle preparation side of slow release carbon source Method, it is characterised in that comprise the following steps：
    （1）, prepare denitrification microorganism
    Cut-in quality is than the microorganism fungus kind Mendoza Pseudomonas albas of 1 ︰ 1, heterotrophic nitrification-aerobic denitrification in liquid medium within Bacterium, inoculum concentration are the 2.5% of fluid nutrient medium weight, under the conditions of 30 ± 1 DEG C, rotating speed are 150r/min, concussion and cultivate 36h, into Denitrification microorganism；
    （2）, prepare microcapsules
    Prepare chitosan solution：Chitosan is dissolved in the acetic acid solution of mass concentration 1% in 60 DEG C of constant temperature stirrings, it is molten into chitosan Liquid, chitosan mass concentration are 1%；
    Prepare sodium alginate soln：90 DEG C of constant temperature stirrings of sodium alginate are soluble in water, it is prepared into the sea that mass concentration is 2 ~ 3% Solution of sodium alginate, then emulsifier span 80 is added in sodium alginate soln, sorbester p17 mass concentration is 0.4%；
    Sodium alginate soln is well mixed with denitrification microorganism with weight than 1 ︰ 1, is placed in pellet fabrication device, instills mass concentration 4% CaCl2Solution, stir, mixing speed 300r/min, mixing time 40min, wrapped up into sodium alginate when being added dropwise Calcium alginate gel, collect calcium alginate gel, it is clean with aseptic water washing, cross 100-150 mesh sieves；
    The calcium alginate gel for crossing 100-150 mesh sieves is put into chitosan solution, secondary solidification 14min is carried out, into micro- glue Capsule, with aseptic water washing 2 ~ 3 times；
    The microcapsules that aseptic water washing is crossed are put into the sodium citrate solution that concentration is 0.10mol/L, carry out intermediate layer marine alga The liquefaction of sour calcium, room temperature liquefaction 7.5min；
    The microcapsules after liquefaction are collected, are cleaned three times with ultra-pure water, vacuum freeze drying 24h ~ 36h, into bio-microcapsule；
    （3）, prepare ecological purified water particle
    Ecological purified water particle is by percentage by weight meter：Flyash 22%, bentonite 30%, vermiculite power 25%, portland cement 9%th, corncob 2%, peanut shell 3% and bio-microcapsule 9% are made up of raw material, wherein, by hydrophilic material, pore-creating character material, stick Mixture, slow release carbon source, bio-microcapsule are well mixed, and are inserted in granulating disc, under 25 ~ 30r/min rotating speeds, use spraying Hydraulic giant constantly sprays water smoke into comminutor, raw material is constantly rolled into 28 ~ 32 mm spherical ecological purified water particle；
    Maintenance：Ecological purified water particle is placed in incubator, continuously conserves 7d, curing temperature is 30 DEG C, humidity 85% Rh, as finished product.
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CN109653001A (en) * 2018-10-25 2019-04-19 安徽亚源印染有限公司 A kind of CVC Dan Sibai fabric and its microcapsules vital staining method
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CN110697907B (en) * 2019-10-18 2022-07-01 长沙理工大学 Immobilized composite flora material and preparation method thereof
CN112897673A (en) * 2019-12-04 2021-06-04 怀化鑫崀峰钙业有限公司 Sewage treatment material with slow release effect
CN111316952A (en) * 2020-03-18 2020-06-23 暨南大学 Sustained-release oxygen-increasing granule and preparation method and application thereof
CN112744907A (en) * 2020-12-21 2021-05-04 江苏聚慧科技有限公司 Method for preparing ecological particles for purifying water quality of river channel
CN112808187A (en) * 2021-01-19 2021-05-18 浙江省林业科学研究院 Automatic equipment and method for producing bubble capsule microbial inoculum
CN115246679A (en) * 2021-10-13 2022-10-28 青岛大学 Preparation method of novel slow-release filler for relieving biological blockage of constructed wetland
CN113800650A (en) * 2021-10-22 2021-12-17 兴源环境科技股份有限公司 Environment-friendly preparation for blue-green algae treatment and preparation method thereof
CN113800650B (en) * 2021-10-22 2023-04-11 兴源环境科技股份有限公司 Environment-friendly preparation for blue-green algae treatment and preparation method thereof
CN114032233A (en) * 2021-11-26 2022-02-11 哈尔滨工业大学（深圳） Slow-release microbial agent and preparation method and application thereof
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