CN108862618B - Microorganism water purification slow-release device and preparation method and application thereof - Google Patents
Microorganism water purification slow-release device and preparation method and application thereof Download PDFInfo
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- CN108862618B CN108862618B CN201810414254.6A CN201810414254A CN108862618B CN 108862618 B CN108862618 B CN 108862618B CN 201810414254 A CN201810414254 A CN 201810414254A CN 108862618 B CN108862618 B CN 108862618B
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
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Abstract
The invention discloses a microorganism water purification slow release device and a preparation method and application thereof, and the microorganism water purification slow release device comprises a protective shell, a protective agent, microorganism bacterium powder and a proliferation accelerant, wherein the protective agent, the microorganism bacterium powder and the proliferation accelerant are contained in the protective shell, and are embedded in a timing slow release layer.
Description
Technical Field
The invention relates to a microorganism water purification slow-release device and a preparation method and application thereof.
Background
The traditional aquaculture is mainly pond aquaculture, because water resources and land resources are increasingly lacked, the pond aquaculture is low in aquaculture density, poor in economic benefit and increasingly higher in cost, and the circulating water aquaculture with a filter is a necessary trend of the current aquaculture. However, the recirculating aquaculture adopts a high-density intensive aquaculture mode, and has great economic benefits and simultaneously faces a plurality of problems. In the process of adopting high-density culture in recirculating water culture, because of excessive feeding, a large amount of residual feed and excrement of cultured animals can generate a large amount of pollutants such as ammonia nitrogen, nitrite, sulfide and the like, thereby not only causing water body pollution, but also influencing the health of the cultured animals. The problems are also the common problems of the circulating water culture systems such as a circulating water temporary culture pond, a display aquarium and the like.
The purification treatment mode of the circulating water aquaculture water body utilizes microorganisms to metabolize excessive baits, excreta, ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and other substances in the water body. The microorganisms for purifying water mainly comprise strains such as bacillus, nitrobacteria, denitrifying bacteria, saccharomycetes, lactic acid bacteria, photosynthetic bacteria and the like. Through the effects of nitrogen fixation, dehydrogenation, carbon fixation, oxidation and the like of microorganisms, harmful substances in the water body can be used as a matrix, so that the content of ammonia nitrogen and nitrite in the water body is obviously reduced, the COD of the water body can be reduced, and the pH value of the water body is stabilized.
The microbial thallus applied to purifying the aquaculture water body is usually sprayed and thrown in a direct mode, but the microbial thallus is not protected by the product, and the microbial thallus can be directly dispersed by water flow, so that the microbial thallus randomly grows on the filter material or in the water body and cannot be fixedly planted at a specific position, and the effect is not obvious. And as the microbial cells age, cause failure of the filtration system. In addition, nutrient substances added in the product in the prior art are not completely protected or even completely exposed, when the product is used, harmful bacteria such as escherichia coli, vibrio and the like directly contact the nutrient substances under the action of water flow, the nutrient substances are robbed and utilized before the water purification microbial thallus is recovered, the propagation of harmful microorganisms is promoted, and carriers such as light calcium carbonate, zeolite powder, ceramsite, bentonite, diatomite, porous clay, porous volcanic rock, zeolite and the like added in the microbial preparation cannot be recovered, so that the secondary pollution of the water body is caused. The invention discloses a microorganism water purification slow-release body and a preparation method thereof (CN 101264985A). the microorganism water purification slow-release body utilizes pores in porous natural ore particles to provide microorganism nidation and culture medium attachment, has certain slow-release capacity, but still has the defects of being dispersed and diluted by water flow, incapable of forming dominant bacteria, utilizing nutrient substances by harmful bacteria, incapable of recycling ore particles and the like because the microorganism bacteria are not protected. The utility model discloses a microbial generator who is used for sewage treatment to have long-term and slow release function (CN206232700U) in "a microbial generator who is used for sewage treatment to have long-term and slow release function" (CN206232700U), but do not protect the device core body, microporous filter body is both ends cavity cylinder simultaneously, so the inside device core body can be gushed into fast to the rivers during use, the microbial bacterial species fast breaks up, culture medium and carrier, and bring into the original harmful bacterium in the water wherein, cause nutrient substance to be robbed in the short time and utilize, when making useful bacterium come back to life or life, no nutrient substance can utilize, can't form the dominant bacteria crowd.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provides a novel microorganism water purification slow-release device and a preparation method and application thereof.
A microbial water purification slow-release device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, and are embedded in a timed slow-release layer, and the preparation method of the microbial water purification slow-release device comprises the following steps:
1) uniformly mixing the microbial powder and the proliferation promoter according to the mass ratio of 1 (1-10000) to prepare a mixture;
2) embedding the mixture into timing release layers with different thicknesses, and embedding at least one layer, wherein the thickness of each timing release layer is 1-1000 mu m;
3) independently embedding at least one proliferation promoter in timing release layers with different thicknesses, wherein the thickness of each timing release layer is 1-1000 mu m;
4) and (3) putting the embedded mixture and the proliferation promoter into a protective shell added with a protective agent according to the mass ratio of 1 (1-10) to obtain the microbial water purification slow-release device.
Further, the microbial powder and the proliferation promoter are uniformly mixed according to the mass ratio of 1:100 to prepare a mixture.
Furthermore, the protective agent is tightly attached to the inside of the protective shell, and the thickness of the protective agent layer is 2-2000 mu m.
Further, the protective agent is tightly adhered to the inside of the shell, and the thickness of the protective agent layer is 200 μm.
Further, the microbial bacteria powder is selected from aerobic bacteria powder, anaerobic bacteria powder and facultative anaerobic bacteria powder.
Further, the protective agent is selected from sodium carboxymethylcellulose, xanthan gum, shellac, sodium alginate, maltodextrin, starch, and soybean protein.
Furthermore, the material of the protective shell is selected from water-filtering paper, woven fabric and filter screen.
Further, the proliferation promoter is selected from skim milk, lactose, yeast extract, peptone, maltose, glucose, xylooligosaccharide, and fructooligosaccharide.
Further, the material of the timing release layer is selected from shellac, sodium alginate, maltodextrin, modified starch, magnesium stearate, sodium carboxymethylcellulose, xanthan gum.
Further, the microorganism water purification slow-release device is also provided with a fixing device.
A filter of a recirculating aquaculture system is characterized in that a microorganism water purification slow-release device is fixed in the filter.
Furthermore, the positions in the filter are different, the types of the microbial bacteria powder contained in the fixed microbial water purification slow-release device are different, the front section of the filter is aerobic bacteria powder, the middle section of the filter is facultative anaerobic bacteria powder, and the rear section of the filter is anaerobic bacteria powder.
The invention has the beneficial effects that:
the protecting shell of the microorganism water purification slow-release device can control the flow of water, so that water flow slowly permeates into the interior, the protective agent fully absorbs water and expands according to set time, and the functions of protecting microorganism cells and proliferation promoters in the interior are achieved.
The existence of the protective agent prevents the strains and the related proliferation accelerant from being dispersed and prevents the prior harmful bacteria in the water body from robbing the accelerant, ensures the quick growth of beneficial microorganisms after germination, increases the bacterial quantity planted on the filtering material and directly enhances the filtering capacity of the filter to the water body.
The timing release layer can release strains in a specific time, so that the microbial inoculum size can be maintained at a high level for a long time after the strains are put into use, and the lasting effect can be half a year. In addition, the timing release layer is independently embedded with the proliferation promoter, so that nutrient substances can be continuously provided after the microbial thalli are recovered and released, the secondary growth of the microbial thalli is promoted, the high concentration of the microbial thalli is maintained, and the microbial thalli are continuously planted on the filter material at the designated position of the filter.
The microorganism water purification slow-release device can be fixed, and meanwhile, devices containing different types of bacteria powder can be arranged according to the characteristics of different positions in a circulating water culture system, particularly a filter, for example, aerobic bacteria are arranged at the front section of the filter to be a main device, facultative anaerobic bacteria are arranged at the middle section of the filter to be a main device, and anaerobic bacteria are arranged at the rear section of the filter to be a main device, so that the filtering effect of the filter in the circulating water culture system is exerted to the maximum.
The filter of the recirculating aquaculture system can be recycled and replaced by a new microorganism water purification slow release device, so that the defect of secondary pollution of water caused by other water purifying agents is overcome, and the high-efficiency water purification effect of the filter can be maintained.
Drawings
FIGS. 1 to 4 are product structure diagrams of the microorganism water purification slow-release device of the invention, wherein 1 is a fixing device, 2 is an extension line, and 3 is a protective shell;
FIGS. 5 to 6 are comparison views of the microbial water-purifying sustained-release device before and during use;
FIG. 7 shows the variation of ammonia nitrogen concentration during the experiment;
FIG. 8 shows the nitrite concentration variation during the experiment;
FIG. 9 shows the variation of nitrate concentration during the experiment;
wherein experimental group 1: control group, no treatment; experimental group 2: the microbial water purification slow-release device of example 1 was put into a filter; experimental group 3: putting the microbial water purifying agent in the form of powder purchased on the market into a filter; experimental group 4: putting the microbial water purifying agent in a liquid form purchased on the market into a filter; experimental group 5: the microorganism water purifying agent with slow release capability purchased in the market is put into the filter.
Detailed Description
The invention is further illustrated with reference to the following figures and examples.
Example 1
A microorganism water purification slow release device comprises the following components: the device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, the device also comprises a fixing device, the fixing device is connected with the protective shell through an extension line, the product structure diagram of the device is shown in figure 1, the fixing device is shown in figure 1, the extension line is shown in figure 2, and the protective shell is shown in figure 3. FIG. 5 is a comparison graph of the microorganism water purification slow-release device before and during use.
The protective shell is made of water filtering paper.
The fixing device comprises holes and adhesive sticker.
The protective agent is formed by mixing sodium carboxymethylcellulose and xanthan gum in a mass ratio of 1:1, and the thickness of the protective agent layer is 200 mu m.
The microbial bacteria powder is aerobic bacteria powder, facultative anaerobic bacteria powder and anaerobic bacteria powder, and the aerobic bacteria powder comprises the following components: bacillus, actinomycetes, nitrobacteria and nitrosobacteria, wherein the facultative anaerobe powder comprises: the lactobacillus powder and the anaerobe powder comprise: photosynthetic bacteria and denitrifying bacteria powder.
The proliferation promoter is prepared by mixing skimmed milk and lactose at a mass ratio of 1: 1.
The timing release layer contains shellac and sodium alginate.
A preparation method of a microorganism water purification slow-release device comprises the following steps:
1) uniformly mixing aerobic bacteria powder and a proliferation promoter in a mass ratio of 1:100 to prepare mixture particles, uniformly dividing the aerobic bacteria powder particles into 3 parts by mass, sequentially embedding the mixture particles by using shellac and sodium alginate to embed the mixture into 3 layers, wherein the thickness of each layer of timing release layer of the same bacteria powder is the same, and the thickness of each layer of timing release layer of the three bacteria powder is respectively 3 microns, 30 microns and 300 microns to prepare an aerobic bacteria composite microbial agent with a specific time release function;
2) preparing a facultative anaerobe compound microbial agent, an anaerobe compound microbial agent and a proliferation promoter with a specific time release function by using the same method;
3) the composite microbial agent and the proliferation accelerant with the specific time release function are placed into a protective shell with the protective agent added according to the mass ratio of 1:2 to respectively prepare the microbial water purification slow release device in the recirculating aquaculture system with aerobic bacteria as the main body, facultative anaerobic bacteria as the main body and anaerobic bacteria as the main body.
According to the internal condition of the circulating water culture filter, the microorganism water purification slow-release device mainly comprising aerobic bacteria, facultative anaerobic bacteria and anaerobic bacteria is fixedly arranged at different positions.
Example 2
A microorganism water purification slow release device comprises the following components: the device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, the device also comprises a fixing device, the fixing device is connected with the protective shell through an extension line, fig. 2 is a product structure diagram of the device, and in the diagram, 1 is the fixing device, 2 is the extension line, and 3 is the protective shell.
The protective shell is made of fabric.
The fixing device comprises holes and adhesive sticker.
The protective agent is formed by mixing shellac and sodium alginate in a mass ratio of 1:1, and the thickness of the protective agent layer is 2 μm.
The microbial bacteria powder is aerobic bacteria powder, facultative anaerobic bacteria powder and anaerobic bacteria powder, and the aerobic bacteria powder comprises the following components: actinomycete, saccharomycete, nitrobacteria and nitrosobacteria powder, wherein the bacteria powder with facultative anaerobe as a main body comprises: the lactic acid bacteria powder, the bacterial powder seed with anaerobe as the main body includes: photosynthetic bacteria powder.
The proliferation promoter is prepared by mixing yeast extract and peptone at a mass ratio of 1: 1.
The timing release layer contains maltodextrin and modified starch.
A preparation method of a microorganism water purification slow-release device comprises the following steps:
1) uniformly mixing aerobic bacteria powder and a proliferation promoter in a mass ratio of 1:1000 to prepare mixture particles, uniformly dividing the aerobic bacteria powder particles into 3 parts according to the mass, sequentially embedding the mixture particles by maltodextrin and modified starch to obtain 2 layers, wherein the thickness of each layer of timing release layer of the same bacteria powder is the same, and the thickness of each layer of timing release layer of each layer of 3 parts of bacteria powder particles is respectively 2 microns, 30 microns and 250 microns to prepare an aerobic bacteria composite microbial agent with a specific time release function;
2) preparing a facultative anaerobe compound microbial agent, an anaerobe compound microbial agent and a proliferation promoter with a specific time release function by using the same method;
3) the composite microbial agent and the proliferation accelerant with the specific time release function are placed into a protective shell with the protective agent added according to the mass ratio of 1:5 to respectively prepare the microbial water purification slow release device in the recirculating aquaculture system with aerobic bacteria as the main body, facultative anaerobic bacteria as the main body and anaerobic bacteria as the main body.
According to the internal condition of the circulating water culture filter, the microorganism water purification slow-release device mainly comprising aerobic bacteria, facultative anaerobic bacteria and anaerobic bacteria is fixedly arranged at different positions.
Example 3
A microorganism water purification slow release device comprises the following components: the device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, the device further comprises a fixing device, the fixing device is connected with the protective shell through an extension line, fig. 3 is a product structure diagram of the device, and in the diagram, 1 is the fixing device, 2 is the extension line, and 3 is the protective shell.
The protective shell is made of a filter screen.
The fixing device contains a hole.
The protective agent is formed by mixing maltodextrin and starch in a mass ratio of 1:1, and the thickness of the protective agent layer is 2000 mu m.
The microbial bacteria powder is aerobic bacteria powder, facultative anaerobic bacteria powder and anaerobic bacteria powder, and the aerobic bacteria powder comprises the following components: bacillus, microzyme, nitrobacteria and nitrosobacteria powder, wherein the bacteria powder taking facultative anaerobe as a main body comprises: the lactic acid bacteria powder, the bacterial powder seed with anaerobe as the main body includes: photosynthetic bacteria and denitrifying bacteria powder.
The proliferation promoter is prepared by mixing maltose and glucose at a mass ratio of 1: 1.
The time release layer comprises magnesium stearate and sodium carboxymethyl cellulose.
A preparation method of a microorganism water purification slow-release device comprises the following steps:
1) uniformly mixing aerobic bacteria powder and a proliferation promoter in a mass ratio of 1:1 to prepare mixture particles, uniformly dividing the aerobic bacteria powder particles into 3 parts according to the mass, sequentially embedding the mixture particles by maltodextrin and modified starch to obtain 2 layers, wherein the thickness of each timed release layer of the same bacteria powder is the same, and the thickness of each timed release layer of each 3 parts of bacteria powder particles is respectively 2 microns, 40 microns and 200 microns to prepare an aerobic bacteria composite microbial agent with a specific time release function;
2) preparing a facultative anaerobe compound microbial agent, an anaerobe compound microbial agent and a proliferation promoter with a specific time release function by using the same method;
3) the composite microbial agent and the proliferation accelerant with the specific time release function are placed into a protective shell with the protective agent added according to the mass ratio of 1:10 to respectively prepare the microbial water purification slow release device in the recirculating aquaculture system with aerobic bacteria as the main body, facultative anaerobic bacteria as the main body and anaerobic bacteria as the main body.
According to the internal condition of the circulating water culture filter, the microorganism water purification slow-release device mainly comprising aerobic bacteria, facultative anaerobic bacteria and anaerobic bacteria is fixedly arranged at different positions.
Example 4
A microorganism water purification slow release device comprises the following components: the device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, the device also comprises a fixing device, the fixing device is connected with the protective shell through an extension line, fig. 4 is a product structure diagram of the device, and in the diagram, 1 is the fixing device, 2 is the extension line, and 3 is the protective shell. FIG. 6 is a comparison graph of the microorganism water purification slow-release device before and during use.
The protective shell is made of water filtering paper.
The fixing device comprises holes and adhesive sticker.
The protective agent is formed by mixing soybean protein and sodium alginate according to the mass ratio of 1:1, and the thickness of the protective agent layer is 1000 mu m.
The microbial bacteria powder is aerobic bacteria powder, facultative anaerobic bacteria powder and anaerobic bacteria powder, and the aerobic bacteria powder comprises the following components: bacillus, nitrobacteria, nitrosation bacterium powder, the fungus powder of facultative anaerobe as the main part includes: the lactic acid bacteria powder, the bacterial powder seed with anaerobe as the main body includes: photosynthetic bacteria and denitrifying bacteria powder.
The proliferation promoter is prepared by mixing xylooligosaccharide and fructo-oligosaccharide in a mass ratio of 1: 1.
The timing release layer contains xanthan gum and shellac.
A preparation method of a microorganism water purification slow-release device comprises the following steps:
1) uniformly mixing aerobic bacteria powder and a proliferation promoter in a mass ratio of 1:5000 to prepare mixture particles, uniformly dividing the aerobic bacteria powder particles into 3 parts according to the mass, sequentially embedding the mixture particles by maltodextrin and modified starch to obtain 2 layers, wherein the thickness of each layer of timing release layer of the same bacteria powder is the same, and the thickness of each layer of timing release layer of each layer of 3 parts of bacteria powder particles is respectively 1 mu m, 30 mu m and 300 mu m to prepare an aerobic bacteria composite microbial agent with a specific time release function;
2) preparing a facultative anaerobe compound microbial agent, an anaerobe compound microbial agent and a proliferation promoter with a specific time release function by using the same method;
3) the composite microbial agent and the proliferation accelerant with the specific time release function are placed into a protective shell with the protective agent added according to the mass ratio of 1:1 to respectively prepare the microbial water purification slow release device in the recirculating aquaculture system with aerobic bacteria as the main body, facultative anaerobic bacteria as the main body and anaerobic bacteria as the main body.
According to the internal condition of the circulating water culture filter, the microorganism water purification slow-release device mainly comprising aerobic bacteria, facultative anaerobic bacteria and anaerobic bacteria is fixedly arranged at different positions.
Example 5
A microorganism water purification slow release device comprises the following components: the device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, the device also comprises a fixing device, the fixing device is connected with the protective shell through an extension line, the product structure diagram of the device is shown in figure 1, the fixing device is shown in figure 1, the extension line is shown in figure 2, and the protective shell is shown in figure 3. FIG. 5 is a comparison graph of the microorganism water purification slow-release device before and during use.
The protective shell is made of fabric.
The fixing device comprises holes and adhesive sticker.
The protective agent is formed by mixing sodium carboxymethylcellulose and shellac in a mass ratio of 1:1, and the thickness of the protective agent layer is 100 μm.
The microbial bacteria powder is aerobic bacteria powder, facultative anaerobic bacteria powder and anaerobic bacteria powder, and the aerobic bacteria powder comprises the following components: actinomycetes, nitrobacteria and nitrosobacteria powder, wherein the bacteria powder with facultative anaerobes as main bodies comprises: the lactic acid bacteria powder, the bacterial powder seed with anaerobe as the main body includes: denitrifying bacteria powder.
The proliferation promoter is prepared by mixing skimmed milk and yeast extract at a mass ratio of 1: 1.
The timing release layer comprises sodium alginate, maltodextrin and modified starch.
A preparation method of a microorganism water purification slow-release device comprises the following steps:
1) uniformly mixing aerobic bacteria powder and a proliferation promoter in a mass ratio of 1:100 to prepare mixture particles, uniformly dividing the aerobic bacteria powder particles into 2 parts by mass, sequentially embedding the 2 parts by using sodium alginate, maltodextrin and modified starch, and embedding the 3 layers together, wherein the thickness of each layer of timing release layer of the same bacteria powder is the same, and the thickness of each layer of timing release layer of the 2 parts of bacteria powder particles is respectively 10 mu m and 100 mu m to prepare the aerobic bacteria composite microbial agent with a specific time release function;
2) preparing a facultative anaerobe compound microbial agent, an anaerobe compound microbial agent and a proliferation promoter with a specific time release function by using the same method;
3) the composite microbial agent and the proliferation accelerant with the specific time release function are placed into a protective shell with the protective agent added according to the mass ratio of 1:8 to respectively prepare the microbial water purification slow release device in the recirculating aquaculture system with aerobic bacteria as the main body, facultative anaerobic bacteria as the main body and anaerobic bacteria as the main body.
According to the internal condition of the circulating water culture filter, the microorganism water purification slow-release device mainly comprising aerobic bacteria, facultative anaerobic bacteria and anaerobic bacteria is fixedly arranged at different positions.
Example 6
A microorganism water purification slow release device comprises the following components: the device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, the device also comprises a fixing device, the fixing device is connected with the protective shell through an extension line, the product structure diagram of the device is shown in figure 1, the fixing device is shown in figure 1, the extension line is shown in figure 2, and the protective shell is shown in figure 3. FIG. 5 is a comparison graph of the microorganism water purification slow-release device before and during use.
The protective shell is made of a filter screen.
The fixing device contains a hole.
The protective agent is formed by mixing xanthan gum and sodium alginate according to the mass ratio of 1:1, and the thickness of the protective agent layer is 200 mu m.
The microbial bacteria powder is aerobic bacteria powder, facultative anaerobic bacteria powder and anaerobic bacteria powder, and the aerobic bacteria powder comprises the following components: yeast, nitrobacteria, nitrosobacteria powder, and the bacteria powder with facultative anaerobe as the main body comprises: the lactic acid bacteria powder, the bacterial powder seed with anaerobe as the main body includes: denitrifying bacteria powder.
The proliferation promoter is prepared by mixing maltose and xylo-oligosaccharide in a mass ratio of 1: 1.
The timing release layer comprises magnesium stearate, sodium carboxymethyl cellulose and shellac.
A preparation method of a microorganism water purification slow-release device comprises the following steps:
1) uniformly mixing aerobic bacteria powder and a proliferation promoter in a mass ratio of 1:100 to prepare mixture particles, uniformly dividing the aerobic bacteria powder particles into 4 parts according to the mass, sequentially embedding the 4 layers by using magnesium stearate, sodium carboxymethyl cellulose and shellac, and embedding the 4 layers together, wherein the thickness of each timed release layer of the same bacteria powder is the same, and the thickness of each timed release layer of the 4 parts of bacteria powder particles is respectively 2 microns, 30 microns, 100 microns and 200 microns to prepare an aerobic bacteria composite microbial agent with a specific time release function;
2) preparing a facultative anaerobe compound microbial agent, an anaerobe compound microbial agent and a proliferation promoter with a specific time release function by using the same method;
3) the composite microbial agent and the proliferation accelerant with the specific time release function are placed into a protective shell with the protective agent added according to the mass ratio of 1:7 to respectively prepare the microbial water purification slow release device in the recirculating aquaculture system with aerobic bacteria as the main body, facultative anaerobic bacteria as the main body and anaerobic bacteria as the main body.
According to the internal condition of the circulating water culture filter, the microorganism water purification slow-release device mainly comprising aerobic bacteria, facultative anaerobic bacteria and anaerobic bacteria is fixedly arranged at different positions.
Example 7
A microorganism water purification slow release device comprises the following components: the device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, the device also comprises a fixing device, the fixing device is connected with the protective shell through an extension line, the product structure diagram of the device is shown in figure 1, the fixing device is shown in figure 1, the extension line is shown in figure 2, and the protective shell is shown in figure 3. FIG. 5 is a comparison graph of the microorganism water purification slow-release device before and during use.
The protective shell is made of filter paper.
The fixing device contains a hole.
The protective agent is formed by mixing maltodextrin and soybean protein according to the mass ratio of 1:1, and the thickness of the protective agent layer is 200 mu m.
The microbial bacteria powder is aerobic bacteria powder, facultative anaerobic bacteria powder and anaerobic bacteria powder, and the aerobic bacteria powder comprises the following components: bacillus, actinomycete, nitrobacteria, nitrosation bacterium powder, the bacterium powder with facultative anaerobe as the main body includes: the lactic acid bacteria powder, the bacterial powder seed with anaerobe as the main body includes: denitrifying bacteria powder.
The proliferation promoter is formed by mixing lactose and peptone in a mass ratio of 1: 1.
The timing release layer contains xanthan gum and sodium alginate.
A preparation method of a microorganism water purification slow-release device comprises the following steps:
1) uniformly mixing aerobic bacteria powder and a proliferation promoter in a mass ratio of 1:100 to prepare mixture particles, uniformly dividing the aerobic bacteria powder particles into 2 parts by mass, sequentially embedding the mixture particles by xanthan gum and sodium alginate to obtain 2 layers, wherein the thickness of each layer of timing release layer of the same bacteria powder is the same, and the thickness of each layer of timing release layer of each layer of 2 parts of bacteria powder particles is respectively 10 microns and 200 microns, so as to prepare the aerobic bacteria composite microbial agent with a specific time release function;
2) preparing a facultative anaerobe compound microbial agent, an anaerobe compound microbial agent and a proliferation promoter with a specific time release function by using the same method;
3) the composite microbial agent and the proliferation accelerant with the specific time release function are placed into a protective shell with the protective agent added according to the mass ratio of 1:4 to respectively prepare the microbial water purification slow release device in the recirculating aquaculture system with aerobic bacteria as the main body, facultative anaerobic bacteria as the main body and anaerobic bacteria as the main body.
According to the internal condition of the circulating water culture filter, the microorganism water purification slow-release device mainly comprising aerobic bacteria, facultative anaerobic bacteria and anaerobic bacteria is fixedly arranged at different positions.
Example 8
A microorganism water purification slow release device comprises the following components: the device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, the device also comprises a fixing device, the fixing device is connected with the protective shell through an extension line, the product structure diagram of the device is shown in figure 1, the fixing device is shown in figure 1, the extension line is shown in figure 2, and the protective shell is shown in figure 3. FIG. 5 is a comparison graph of the microorganism water purification slow-release device before and during use.
The protective shell is made of fabric.
The fixing device comprises holes and adhesive sticker.
The protective agent is formed by mixing starch and sodium alginate according to the mass ratio of 1:1, and the thickness of the protective agent layer is 2000 mu m.
The microbial bacteria powder is aerobic bacteria powder, facultative anaerobic bacteria powder and anaerobic bacteria powder, and the aerobic bacteria powder comprises the following components: bacillus, actinomycete, yeast, nitrobacteria, nitrosation bacterium powder, the bacterium powder with facultative anaerobe as the main body includes: the lactic acid bacteria powder, the bacterial powder seed with anaerobe as the main body includes: photosynthetic bacteria powder.
The proliferation promoter is prepared by mixing glucose and fructo-oligosaccharide in a mass ratio of 1: 1.
The timing release layer contains shellac and maltodextrin.
A preparation method of a microorganism water purification slow-release device comprises the following steps:
1) uniformly mixing aerobic bacteria powder and a proliferation promoter in a mass ratio of 1:100 to prepare mixture particles, uniformly dividing the aerobic bacteria powder particles into 3 parts according to the mass, sequentially embedding the mixture particles by shellac and maltodextrin, and embedding the mixture into 2 layers, wherein the thickness of each layer of timing release layer of the same bacteria powder is the same, and the thickness of each layer of timing release layer of the 3 parts of bacteria powder particles is respectively 2 microns, 50 microns and 200 microns to prepare an aerobic bacteria composite microbial agent with a specific time release function;
2) preparing a facultative anaerobe compound microbial agent, an anaerobe compound microbial agent and a proliferation promoter with a specific time release function by using the same method;
3) the composite microbial agent and the proliferation accelerant with the specific time release function are placed into a protective shell with the protective agent added according to the mass ratio of 1:6 to respectively prepare the microbial water purification slow release device in the recirculating aquaculture system with aerobic bacteria as the main body, facultative anaerobic bacteria as the main body and anaerobic bacteria as the main body.
According to the internal condition of the circulating water culture filter, the microorganism water purification slow-release device mainly comprising aerobic bacteria, facultative anaerobic bacteria and anaerobic bacteria is fixedly arranged at different positions.
Example 9
A microorganism water purification slow release device comprises the following components: the device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, the device also comprises a fixing device, the fixing device is connected with the protective shell through an extension line, the product structure diagram of the device is shown in figure 1, the fixing device is shown in figure 1, the extension line is shown in figure 2, and the protective shell is shown in figure 3. FIG. 5 is a comparison graph of the microorganism water purification slow-release device before and during use.
The protective shell is made of a filter screen.
The fixing device comprises holes and adhesive sticker.
The protective agent is formed by mixing sodium carboxymethylcellulose and starch in a mass ratio of 1:1, and the thickness of the protective agent layer is 2000 mu m.
The microbial bacteria powder is aerobic bacteria powder, facultative anaerobic bacteria powder and anaerobic bacteria powder, and the aerobic bacteria powder comprises the following components: actinomycete, yeast, nitrobacteria and nitrosobacteria powder, wherein the bacteria powder taking facultative anaerobes as main bodies comprises: the lactic acid bacteria powder, the bacterial powder seed with anaerobe as the main body includes: photosynthetic bacteria and denitrifying bacteria powder.
The proliferation promoter is prepared by mixing skim milk and peptone at a mass ratio of 1: 1.
The timing release layer comprises sodium alginate and modified starch.
A preparation method of a microorganism water purification slow-release device comprises the following steps:
1) uniformly mixing aerobic bacteria powder and a proliferation promoter in a mass ratio of 1:100 to prepare mixture particles, uniformly dividing the aerobic bacteria powder particles into 3 parts according to the mass, sequentially embedding the mixture particles by using sodium alginate and modified starch to embed 2 layers, wherein the thickness of each layer of timing release layer of the same bacteria powder is the same, and the thickness of each layer of timing release layer of the 3 parts of bacteria powder particles is 1 mu m, 60 mu m and 180 mu m respectively to prepare the aerobic bacteria composite microbial agent with a specific time release function;
2) preparing a facultative anaerobe compound microbial agent, an anaerobe compound microbial agent and a proliferation promoter with a specific time release function by using the same method;
3) the composite microbial agent and the proliferation accelerant with the specific time release function are placed into a protective shell with the protective agent added according to the mass ratio of 1:5 to respectively prepare the microbial water purification slow release device in the recirculating aquaculture system with aerobic bacteria as the main body, facultative anaerobic bacteria as the main body and anaerobic bacteria as the main body.
According to the internal condition of the circulating water culture filter, the microorganism water purification slow-release device mainly comprising aerobic bacteria, facultative anaerobic bacteria and anaerobic bacteria is fixedly arranged at different positions.
Function detection of microorganism water purification slow-release device
In order to examine the enhancement effect of the microorganism water purification slow release device on the filtration system in the recirculating aquaculture system, the following experiment is set.
Choose 5 groups of aquarium of taking the filter for use, every group is raised 3 length and is 20 ~ 25cm, healthy carp, and the equivalent fodder is normally thrown to feed every day to carry out following processing at the experiment initiation:
experimental group 1: no treatment is carried out;
experimental group 2: putting the microorganism water purification slow-release device in the recirculating aquaculture system of the embodiment 1 into a filter;
experimental group 3: putting the microbial water purifying agent in the form of powder purchased on the market into a filter;
experimental group 4: putting the microbial water purifying agent in a liquid form purchased on the market into a filter;
experimental group 5: the microorganism water purifying agent with slow release capability purchased in the market is put into the filter.
The experimental results are shown in fig. 7, 8 and 9, and experiments show that the concentrations of ammonia nitrogen, nitrite and nitrate in the water body of the experimental group 1 are continuously increased along with the progress of the experiments. The concentrations of ammonia nitrogen, nitrite and nitrate in the water bodies of the experimental groups 2, 3, 4 and 5 at the initial stage of the putting are all reduced to a certain extent relative to the experimental group 1, but the concentrations of ammonia nitrogen, nitrite and nitrate in the water bodies of the experimental groups 3, 4 and 5 begin to rise slowly with the lapse of time and the dilution effect of the water bodies, which shows that the service life of the products is short, the products need to be put into use for a plurality of times, inconvenience is brought to the use, and the cost is increased. Although the experimental group 5 has a slow release function, the water purification capacity of the experimental group is superior to that of the experimental groups 3 and 4, the concentrations of ammonia nitrogen, nitrite and nitrate in the water body start to continuously rise again after 30 days of the experiment due to the influence of dilution of the water body, and the ammonia nitrogen, the nitrite and the nitrate need to be thrown again. In the whole experiment process of the experiment group 2, the water purification capacity is always kept at a higher level, the concentration indexes of ammonia nitrogen, nitrite and nitrate do not obviously increase, and the secondary feeding is not needed within a long period of time after the feeding, so that great convenience is provided for use.
Claims (6)
1. A microbial water purification slow-release device comprises a protective shell, a protective agent, microbial powder and a proliferation promoter, wherein the protective agent, the microbial powder and the proliferation promoter are contained in the protective shell, and are embedded in a timed slow-release layer, and the preparation method of the microbial water purification slow-release device comprises the following steps:
1) uniformly mixing the microbial powder and the proliferation promoter according to the mass ratio of 1 (1-10000) to prepare a mixture;
2) embedding the mixture into timing release layers with different thicknesses, wherein at least one layer is embedded, and the thickness of each timing release layer is 1-1000 mu m;
3) independently embedding at least one proliferation promoter in timing release layers with different thicknesses, wherein the thickness of each timing release layer is 1-1000 mu m;
4) putting the embedded mixture and the proliferation promoter into a protective shell added with a protective agent according to the mass ratio of 1 (1-10) to obtain a microorganism water purification slow-release device;
the timing release layer material is selected from shellac, sodium alginate, maltodextrin, modified starch, magnesium stearate, sodium carboxymethylcellulose, xanthan gum;
the protective agent is tightly attached to the inside of the protective shell, and the thickness of the protective agent layer is 2-2000 mu m;
the protective agent is selected from sodium carboxymethylcellulose, xanthan gum, shellac, sodium alginate, maltodextrin, starch, and soybean protein;
the proliferation promoter is selected from skimmed milk, lactose, yeast extract, peptone, maltose, glucose, xylooligosaccharide, and fructooligosaccharide.
2. The microbial water purification slow-release device according to claim 1, wherein the microbial bacteria powder is selected from one of aerobic bacteria powder, anaerobic bacteria powder and facultative anaerobic bacteria powder.
3. The microbial water purification slow-release device of claim 1, wherein the material of the protective shell is selected from one of water-filtering paper, woven fabric and a filter screen.
4. The microbial water purification slow release device of claim 1, further comprising a fixing device.
5. A filter of a recirculating aquaculture system, wherein the microorganism water purification slow-release device as claimed in any one of claims 1 to 4 is fixed in the filter.
6. The filter of the circulating water aquaculture system of claim 5, wherein the filter has different inner positions, the fixed microorganism water purification slow release devices contain different types of microorganism bacteria, the front section of the filter is aerobic bacteria powder, the middle section of the filter is facultative anaerobic bacteria powder, and the rear section of the filter is anaerobic bacteria powder.
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| CN111434623A (en) * | 2019-01-12 | 2020-07-21 | 广州金水动物保健品有限公司 | Tablet for improving culture environment of culture pond and preparation method and application thereof |
| CN109775866B (en) * | 2019-03-13 | 2022-07-08 | 南京工业大学 | Preparation method of composite sustained-release microcapsule preparation for treating ammonia nitrogen in aquaculture water |
| CN110790396A (en) * | 2019-11-21 | 2020-02-14 | 上海泰缘生物科技股份有限公司 | Biological floccule and strain slow-release device and system thereof |
| CN113025520A (en) * | 2021-03-15 | 2021-06-25 | 上海良延环保科技发展有限公司 | Brick capable of slowly releasing strain, slowly releasing strain and water purification method using same |
| CN113582351A (en) * | 2021-07-21 | 2021-11-02 | 浙江桃花源环保科技有限公司 | Water treatment bioreactor based on layered structure |
| CN115465949B (en) * | 2022-07-29 | 2024-01-02 | 天津正达科技有限责任公司 | Immobilized microorganism composite material with core-shell structure and preparation method thereof |
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