CN112807257A - Preparation method of radix Paeoniae extract - Google Patents
Preparation method of radix Paeoniae extract Download PDFInfo
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- 239000000284 extract Substances 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 120
- 238000000605 extraction Methods 0.000 claims abstract description 40
- 235000006484 Paeonia officinalis Nutrition 0.000 claims abstract description 32
- 241000736199 Paeonia Species 0.000 claims abstract description 26
- 239000003480 eluent Substances 0.000 claims abstract description 22
- 239000011347 resin Substances 0.000 claims abstract description 21
- 229920005989 resin Polymers 0.000 claims abstract description 21
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 18
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- 238000001035 drying Methods 0.000 claims abstract description 6
- 238000005406 washing Methods 0.000 claims abstract description 6
- 239000006228 supernatant Substances 0.000 claims abstract description 5
- 238000002791 soaking Methods 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 23
- 238000001179 sorption measurement Methods 0.000 claims description 6
- 244000236658 Paeonia lactiflora Species 0.000 claims description 4
- 235000008598 Paeonia lactiflora Nutrition 0.000 claims description 4
- 235000011837 pasties Nutrition 0.000 claims description 4
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 4
- 229940084032 paeonia lactiflora root extract Drugs 0.000 claims 5
- 238000005292 vacuum distillation Methods 0.000 claims 1
- 241001106477 Paeoniaceae Species 0.000 abstract description 6
- YKRGDOXKVOZESV-WRJNSLSBSA-N Paeoniflorin Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C)OC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-WRJNSLSBSA-N 0.000 description 14
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- LATYEZNGPQKAIK-HRCYFWENSA-N Benzoylpaeoniflorin Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](COC(=O)C=2C=CC=CC=2)O1)O)C)OC(=O)C1=CC=CC=C1 LATYEZNGPQKAIK-HRCYFWENSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
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- NLSMPWTZYGSAEU-CYUNEVMDSA-N (2s,3r,4s,5s,6r)-2-[7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-3-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromenylium-5-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol;chloride Chemical compound [Cl-].C1=C(O)C(OC)=CC(C=2C(=CC=3C(O[C@H]4[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O)=CC(O)=CC=3[O+]=2)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)=C1 NLSMPWTZYGSAEU-CYUNEVMDSA-N 0.000 description 1
- QQUHMASGPODSIW-UHFFFAOYSA-N Albiflorin Natural products C=1C=CC=CC=1C(=O)OCC12C(=O)OC3(C)CC(O)C1CC32OC1OC(CO)C(O)C(O)C1O QQUHMASGPODSIW-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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Abstract
The invention discloses a preparation method of a peony root extract, which comprises the following steps: slicing, drying and crushing fresh radix paeoniae alba to obtain radix paeoniae alba raw materials; soaking the radix Paeoniae raw material in ethanol solution, simultaneously performing ultrasonic extraction, centrifuging, and collecting supernatant as extractive solution; removing ethanol in the extracting solution by adopting a reduced pressure distillation mode to obtain a peony root extract; passing the radix Paeoniae extract through macroporous adsorbent resin, and sequentially washing the macroporous adsorbent resin with eluents with sequentially increasing concentrations to obtain eluate; and removing the eluent in the eluent by adopting a reduced pressure distillation mode to obtain the purified paeony root extract. The invention can not only improve the extraction rate, but also protect the effective components from being damaged to the maximum extent.
Description
Technical Field
The invention relates to the field of extraction, and particularly relates to a preparation method of a peony root extract.
Background
With the continuous progress of society and the continuous improvement of living standard, people have more and more strict pursuit of beauty, and the cosmetics are expected to have other curative effects, such as treating skin allergy symptoms, improving whitening effect, strengthening skin moisture retention and the like, while protecting skin.
The peony is a Chinese medicinal material, the extract of the peony is total paeoniflorin, the total paeoniflorin is a mixture of physiological functional components such as paeoniflorin (paeoniflorin), hydroxy-paeoniflorin (hydroxy-paeoniflorin), paeoniflorin (paeonin), albiflorin (albicflorin), benzoyl paeoniflorin (benzoyl paeoniflorin) and the like, and the extract has antibacterial property, activation property, moisture retention property and the like, and can play roles of resisting allergy, moisture retention, whitening and resisting aging when being applied to cosmetics.
In the prior art, the extraction method is adopted to extract the paeony root extract, and in the method, the extraction time is too long, the extraction of effective components is insufficient, and the extraction rate of the paeony root extract is low.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a preparation method of a peony root extract, which improves the extraction rate by optimizing process parameters.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a preparation method of a radix paeoniae alba extract comprises the following steps:
s1: slicing, drying and crushing fresh radix paeoniae alba to obtain radix paeoniae alba raw materials;
s2: soaking the radix paeoniae alba raw material in an ethanol solution, simultaneously carrying out ultrasonic extraction, and taking supernate as an extracting solution after the ultrasonic extraction is finished;
s3: removing ethanol in the extracting solution by adopting a reduced pressure distillation mode to obtain a peony root extract;
s4: passing the radix Paeoniae extract through macroporous adsorbent resin, and washing the macroporous adsorbent resin with eluent to obtain eluent;
s5: removing the eluent in the eluent by adopting a reduced pressure distillation mode to obtain a concentrated solution, and carrying out vacuum freeze drying on the concentrated solution to obtain a purified powdery or pasty radix paeoniae alba extract.
The embodiment of the invention has the following beneficial effects:
the invention adopts ethanol solution as leaching solution, combines an ultrasonic-assisted extraction method to prepare the peony root extract, breaks cell walls by utilizing the cavitation of ultrasonic waves, and enables the ethanol solution to fully enter cells to extract effective components, thereby improving the extraction rate.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Wherein:
FIG. 1 is a line contour plot of the effect of ethanol concentration and extraction time on peony extraction.
FIG. 2 is a graph of response of ethanol concentration and extraction time on the effect of peony extraction.
FIG. 3 is a line contour plot of the effect of ethanol concentration and liquid-to-liquid ratio on peony extraction.
FIG. 4 is a graph of the response of the ethanol concentration and liquid feed ratio on the peony extraction.
FIG. 5 is a contour plot of the effect of extraction time and liquid feed ratio on peony extraction.
FIG. 6 is a graph of response of the extraction time and liquid feed ratio on the effect of peony extraction.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention discloses a preparation method of a peony root extract,
s1) providing a radix paeoniae raw material. Preferably, the radix Paeoniae material is radix Paeoniae alba radix Paeoniae. The radix Paeoniae raw material can be prepared by slicing fresh radix Paeoniae, oven drying and pulverizing.
S2) soaking the radix Paeoniae raw material in the leaching solution, and simultaneously performing ultrasonic extraction for 10-30 min, and taking the supernatant as the extracting solution; wherein the lixivium is an ethanol solution with the volume concentration of 50-90%, and the mass ratio (namely the liquid-material ratio) of the volume of the ethanol solution to the peony root raw material is 5-15 mL/g.
In this process, the ultrasonic power is preferably 240W to 320W.
Because the concentration, the using amount and the ultrasonic effect of the leaching solution have different influences on the extracts with different components, the extraction rate can be reduced due to the fact that the concentration of the leaching solution is too low, but the effective components of the extracts can be damaged due to the fact that the concentration of the leaching solution is too high, the extraction rate is reduced, and the effective components of the extracts can be damaged due to the fact that the ultrasonic time is too long, the factors are further optimized, the extraction rate is improved, and meanwhile the effective components are protected to the maximum extent. Preferably, the ultrasonic extraction time is 10min to 30min, the volume concentration of the ethanol solution is 50 percent to 90 percent, and the ratio of the volume of the ethanol solution to the mass of the radix paeoniae alba raw material is 5mL/g to 15 mL/g.
S3) removing ethanol in the extracting solution by adopting a reduced pressure distillation method to obtain the peony root extract.
Preferably, the pressure of the reduced pressure distillation is 1.3kPa to 2.0kPa, and the temperature is 30 ℃ to 50 ℃. The lower the pressure, the better, the lower the temperature, avoid the effective component to be heated and decomposed.
In order to optimize the concentration, the liquid-material ratio and the ultrasonic time of the ethanol solution and obtain a better extraction rate, the concentration, the liquid-material ratio and the ultrasonic time of the ethanol solution are respectively optimized through experiments.
The concentration of the ethanol solution is optimized:
accurately weighing 8 parts of 2.0g white paeony root powder in an erlenmeyer flask, and respectively adding 30mL of 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% ethanol solutions, and numbering for later use. And putting the eight conical flasks into an ultrasonic cleaning machine, setting the ultrasonic power to be 280W, performing ultrasonic extraction for 30min, then performing reduced pressure distillation on 10mL of supernate respectively, removing ethanol to obtain extracting solutions, diluting the extracting solutions, and respectively measuring the maximum absorbance of each extracting solution, wherein the pressure of the reduced pressure distillation is 1.5kPa, the temperature is 40 ℃, and the time is 30 min. Triplicate determinations were averaged.
Referring to fig. 1 and 2, the greater the absorbance, the higher the extraction rate of the extract, and the test shows that the absorbance is the greatest at an ethanol concentration of 80%, the absorbance increases with the increase of the concentration when the ethanol concentration is less than 80%, and the absorbance decreases sharply when the ethanol concentration is more than 80%, and the preferable range of the ethanol concentration is 50% to 90%.
Optimizing the liquid-material ratio:
accurately weighing 5 parts of 2.0g white paeony root powder in an erlenmeyer flask, and respectively adding 10mL, 20mL, 30mL, 40mL and 50mL of 80% ethanol solution for standby application. And putting the five conical flasks into an ultrasonic cleaning machine, setting the ultrasonic power to be 280W, performing ultrasonic extraction for 30min, then performing reduced pressure distillation on 10mL of supernate respectively, removing ethanol to obtain extracting solutions, diluting the extracting solutions, and respectively measuring the maximum absorbance of each extracting solution, wherein the pressure of the reduced pressure distillation is 1.5kPa, the temperature is 40 ℃, and the time is 30 min. Triplicate determinations were averaged.
Referring to fig. 3 and 4, the absorbance is the largest when the liquid-to-material ratio is 10mL/g, the absorbance increases with the increase of the liquid-to-material ratio when the liquid-to-material ratio is less than 10mL/g, and the absorbance gradually decreases when the liquid-to-material ratio is greater than 10mL/g, and the preferable range of the liquid-to-material ratio is 5mL/g to 15 mL/g.
Optimizing ultrasound time
Accurately weighing 5 parts of 2.0g white paeony root powder in an erlenmeyer flask, and respectively adding 20mL 80% ethanol solution for numbering for later use. Placing five conical flasks in an ultrasonic cleaning machine, setting ultrasonic power at 280W, extracting for 10min, 20min, 30min, 40min and 50min respectively, distilling 10mL of supernatant under reduced pressure at 40 deg.C and 1.5kPa for 30min, removing ethanol to obtain extractive solution, diluting the extractive solution, and measuring maximum absorbance of each extractive solution. Triplicate determinations were averaged.
Referring to fig. 5 and 6, the absorbance is maximum when the sonication time is 20min, increases with the increase of the sonication time when the sonication time is less than 20min, and sharply decreases when the sonication time is greater than 20min, with the preferred range of the sonication time being 10min to 30 min.
And optimizing by using a response surface method to obtain the optimal condition of the peony ultrasonic extraction process. The invention designs a response surface experiment by Design-Expert software and by using a Box-Behnken center combination Design principle. On the basis of the single-factor experiment result, 3 factors of extraction time, liquid-material ratio and concentration of the ethanol solution are selected as variables, and the absorbance is used as a response value to design an experiment. The experimental factors and horizontal codes are shown in table 1, and the design and results of the central optimization combined test are shown in table 2.
Table 1: Box-Benhnken test factor code and level table
Table 2: central optimization combined experimental design and results
Variance analysis and quadratic polynomial regression fitting are carried out on experimental data by using Design-expert8.0.6 software, the result is shown in table 3, a regression model P is less than 0.0001, the model is very remarkable, a mismatching term P is 0.2095 and is not remarkable, and the fitting degree of the model is good.
Fitted multiple quadratic regression equation:
R＝-4.25712+0.27170A+0.45180B+0.97510C+0.000025AB+0.00015AC+0.0065BC-0.00164875A2-0.012845B2-0.05398C2
in the formula, A is the concentration of an ethanol solution, B is ultrasonic time, and C is the liquid-material ratio.
The optimal ultrasonic extraction process of the peony by using Design-Expert8.0.6 software optimization is as follows: the concentration of the ethanol solution is 83.01%, the ultrasonic time is 20.29min, and the extraction rate of the total paeoniflorin under the condition is 16.6605mg/mL, wherein the liquid-to-material ratio is 10.37 mL/g.
Preferably, the concentration of the ethanol solution is 82-84%, the ultrasonic time is 19.3-21.3 min, and the mass ratio (liquid-material ratio) of the volume of the ethanol solution to the radix paeoniae alba raw material is 9.4-11.4 mL/g.
Table 3: regression equation analysis of variance results
Note: indicates that the difference is extremely significant (P < 0.001); indicates significant differential height (P < 0.01); indicates significant differences (P < 0.05).
Contour diagrams and response surface 3D diagrams of ultrasound extraction of radix Paeoniae are shown in FIGS. 1-6. As can be seen from the figure: the influence of the ethanol concentration on the extraction effect is the largest, the liquid-material ratio is the second, and the influence of the ultrasonic time on the extraction effect of the Chinese herbaceous peony is the smallest; the interaction of the liquid-material ratio and the extraction time has a large influence on the extraction effect of the Chinese herbaceous peony, and the interaction of the ethanol concentration and the liquid-material ratio is the second, and the interaction of the ethanol concentration and the extraction time is the least obvious.
And (3) verification test:
in order to verify the reliability of the result obtained by the response surface method, the optimal process condition of the model optimization is verified. Considering the feasibility and the convenience of the experiment, the process parameters are selected as follows: the ethanol concentration is 83.00%, the ultrasonic time is 20.3min, the liquid-material ratio is 10.40mL/g, and the theoretical extraction rate under the condition is 16.6603 mg/g. The experimental results are shown in table 4, which shows that the equation fits well with the real test conditions, and the optimized process is reliable and feasible, so that the optimal process conditions for preparing the paeonia lactiflora extract by ethanol extraction assisted ultrasonic extraction are as follows: the ethanol concentration is 83.00%, the ultrasonic time is 20.3min, and the liquid-material ratio is 10.40 mL/g.
Table 4: verification test table
S4) leading the peony root extract to pass through macroporous absorption resin, and washing the macroporous absorption resin by eluent with gradually increasing concentration to obtain eluent.
More preferably, the washing of the macroporous adsorbent resin with the eluent is carried out by: and sequentially adopting ethanol solutions with volume concentrations of 30%, 50%, 70% and 95% to wash the macroporous adsorption resin.
S5) removing the eluent in the eluent by adopting a reduced pressure distillation method to obtain the purified paeonia lactiflora pall extract.
Preferably, the pressure of the reduced pressure distillation is 1.3kPa to 2.0kPa, and the temperature is 30 ℃ to 50 ℃.
The following are specific examples.
Example 1
Cleaning radix Paeoniae alba and radix Paeoniae, slicing, drying, and pulverizing into 40 mesh powder.
Accurately weighing 2.88g of radix paeoniae alba and radix paeoniae alba powder into an erlenmeyer flask, adding 30mL of 83% ethanol solution, putting the erlenmeyer flask into an ultrasonic cleaning machine, setting the ultrasonic power to be 280W, carrying out ultrasonic extraction for 20.3min, taking supernate, carrying out reduced pressure distillation under the pressure of 1.5kPa at the temperature of 40 ℃ for 30min, and removing ethanol to obtain an extracting solution.
Injecting the extracting solution into macroporous resin HPD-450, adding the extracting solution with the proportion of not more than 30mL into 1g of macroporous resin HPD-450 for adsorption, shaking for 1h, rinsing the macroporous resin HPD-450 with distilled water after adsorption saturation, removing impurities, filtering, adding 80mL of ethanol solution with the volume concentration of 70%, and eluting the peony root extract adsorbed by the macroporous resin to obtain eluent.
Distilling under reduced pressure at 40 deg.C under 1.5kPa to remove ethanol and further remove water to obtain concentrated pasty radix Paeoniae extract, and vacuum freeze drying to obtain powdered radix Paeoniae extract (47.8 mg), which is radix Paeoniae total glycoside.
The extraction rate of the total paeoniflorin in the embodiment is about 16.6mg/g through calculation.
Example 2
Example 2 is basically the same as example 1, except that in example 2, the ethanol solution concentration is 82%, the ultrasonic time is 21.3min, and the liquid-to-material ratio is 11.4 mL/g. The obtained powdered radix Paeoniae extract is 47.23 mg.
The extraction rate of the total paeoniflorin in the embodiment is about 16.4mg/g through calculation.
Example 3
Example 3 is substantially the same as example 1 except that in example 3, the ethanol solution concentration is 84%, the ultrasonic time is 19.3min, and the liquid-to-material ratio is 9.4 mL/g. The obtained powdered radix Paeoniae extract is 47.52 mg.
The extraction rate of the total paeoniflorin in the embodiment is about 16.5mg/g through calculation.
Comparative example
Cleaning radix Paeoniae alba and radix Paeoniae, slicing, drying, and pulverizing into 40 mesh powder.
Accurately weighing 2.88g of radix Paeoniae alba and radix Paeoniae powder in a conical flask, adding 83% ethanol solution 30mL, extracting for 20.3min, collecting supernatant, distilling under reduced pressure of 1.5kPa at 40 deg.C for 30min, and removing ethanol to obtain extractive solution.
Injecting the extract into macroporous resin HPD-450, adding the extract into the macroporous resin HPD-450 according to the proportion that the macroporous resin HPD-450 is 1g and the extract is not more than 30mL for adsorption, shaking for 1h, rinsing the macroporous resin HPD-450 with distilled water after adsorption saturation, removing impurities, filtering, adding into 80mL of 70% ethanol solution, and eluting the peony root extract adsorbed by the macroporous resin to obtain eluent.
Distilling under reduced pressure at 40 deg.C under 1.5kPa to remove ethanol and further remove water to obtain concentrated pasty radix Paeoniae extract, and vacuum freeze drying to obtain powdered radix Paeoniae extract 18.144mg, which is radix Paeoniae total glycoside.
The extraction rate of the total paeoniflorin in the comparative example is about 6.3mg/g through calculation,
the data of the examples 1-3 and the comparative example show that the extraction method can greatly improve the extraction rate of the total paeoniflorin, the extraction rate of the total paeoniflorin in the example 1 reaches 16.6mg/g under the condition of adopting the most preferable parameters, the extraction rate is very close to the theoretical value, and the loss rate of the total paeoniflorin in the process of extracting the total paeoniflorin in the example 1 is very little.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the claims. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A preparation method of a peony root extract is characterized by comprising the following steps:
s1: slicing, drying and crushing fresh radix paeoniae alba to obtain radix paeoniae alba raw materials;
s2: soaking the radix Paeoniae raw material in ethanol solution, simultaneously performing ultrasonic extraction, centrifuging after completion, and taking supernatant as extractive solution;
s3: removing ethanol in the extracting solution by adopting a reduced pressure distillation mode to obtain a peony root extract;
s4: passing the radix Paeoniae extract through macroporous adsorbent resin, and washing the macroporous adsorbent resin with eluent to obtain eluent;
s5: removing the eluent in the eluent by adopting a reduced pressure distillation mode to obtain a concentrated solution, and carrying out vacuum freeze drying on the concentrated solution to obtain a purified powdery or pasty radix paeoniae alba extract.
2. The method for preparing the paeonia lactiflora root extract as claimed in claim 1, wherein the ultrasonic extraction time is 10min to 30min, the volume concentration of the ethanol solution is 50% to 90%, and the ratio of the volume of the ethanol solution to the mass of the paeonia lactiflora root raw material is 5mL/g to 15 mL/g.
3. The method for preparing the paeonia lactiflora root extract as claimed in claim 2, wherein the volume concentration of the ethanol solution is 82-84%, the ratio of the volume of the ethanol solution to the mass of the paeonia lactiflora root raw material is 9.4-11.4 mL/g, and the ultrasonic extraction time is 19.3-21.3 min.
4. The method for preparing radix Paeoniae extract as claimed in claim 3, wherein the volume concentration of the ethanol solution is 83%, the ratio of the volume of the ethanol solution to the mass of the radix Paeoniae raw material is 10.4mL/g, and the ultrasonic extraction time is 20.3 min.
5. The method for preparing paeonia lactiflora root extract according to claim 1, wherein the ultrasonic power for ultrasonic extraction is 240W-320W.
6. The method of claim 1, wherein the eluent is an ethanol solution.
7. The method for preparing radix Paeoniae extract as claimed in claim 6, wherein the washing of the macroporous adsorbent resin with eluent is performed by: and sequentially adopting ethanol solutions with volume concentrations of 30%, 50%, 70% and 95% to wash the macroporous adsorption resin.
8. The method of claim 1, wherein the reduced pressure distillation is performed at a pressure of 1.3kPa to 2.0kPa and a temperature of 30 ℃ to 50 ℃ during the removal of the ethanol in the extraction solution.
9. The method of claim 1, wherein the vacuum degree of the vacuum distillation is 1.3kPa to 2.0kPa and the temperature is 30 ℃ to 50 ℃ during the removing of the eluent in the eluent.
10. The method of claim 1, further comprising concentrating and drying the purified paeonia lactiflora root extract after obtaining the purified paeonia lactiflora root extract.
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| CN115677796A (en) * | 2022-11-23 | 2023-02-03 | 四川聚元药业集团有限公司 | Method for extracting paeoniflorin from white paeony root |
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| CN102492005A (en) * | 2011-12-12 | 2012-06-13 | 苏州大学 | Method for preparing paeoniflorin and albiflorin |
| CN103044503A (en) * | 2012-12-24 | 2013-04-17 | 浙江工业大学 | Method for rapidly and efficiently extracting paeoniflorin and albiflorin |
| CN109627271A (en) * | 2018-12-11 | 2019-04-16 | 四川农业大学 | It is a kind of quickly from Chinese herbaceous peony cauline leaf extraction purification Paeoniflorin technique |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102492005A (en) * | 2011-12-12 | 2012-06-13 | 苏州大学 | Method for preparing paeoniflorin and albiflorin |
| CN103044503A (en) * | 2012-12-24 | 2013-04-17 | 浙江工业大学 | Method for rapidly and efficiently extracting paeoniflorin and albiflorin |
| CN109627271A (en) * | 2018-12-11 | 2019-04-16 | 四川农业大学 | It is a kind of quickly from Chinese herbaceous peony cauline leaf extraction purification Paeoniflorin technique |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115677796A (en) * | 2022-11-23 | 2023-02-03 | 四川聚元药业集团有限公司 | Method for extracting paeoniflorin from white paeony root |
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