CN112799225A - Smart phone microscopic system based on intensity transmission equation - Google Patents
Smart phone microscopic system based on intensity transmission equation Download PDFInfo
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- CN112799225A CN112799225A CN202011639444.1A CN202011639444A CN112799225A CN 112799225 A CN112799225 A CN 112799225A CN 202011639444 A CN202011639444 A CN 202011639444A CN 112799225 A CN112799225 A CN 112799225A
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- 230000005540 biological transmission Effects 0.000 title claims abstract description 29
- 238000000386 microscopy Methods 0.000 claims 5
- 238000000034 method Methods 0.000 abstract description 11
- 238000003384 imaging method Methods 0.000 abstract description 7
- 239000000523 sample Substances 0.000 description 19
- 238000011084 recovery Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000000399 optical microscopy Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/362—Mechanical details, e.g. mountings for the camera or image sensor, housings
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04M—TELEPHONIC COMMUNICATION
- H04M1/00—Substation equipment, e.g. for use by subscribers
- H04M1/02—Constructional features of telephone sets
- H04M1/0202—Portable telephone sets, e.g. cordless phones, mobile phones or bar type handsets
- H04M1/026—Details of the structure or mounting of specific components
- H04M1/0264—Details of the structure or mounting of specific components for a camera module assembly
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Abstract
The invention provides an intelligent mobile phone microscopic system based on an intensity transmission equation, which comprises an intelligent mobile phone, a connecting device, a microscope, a miniature electric actuator, a remote controller and a support, wherein the intelligent mobile phone is connected with the connecting device; the utility model discloses a miniature electronic device, including connecting device, camera, microscope, remote controller, flash light hole, microscope, eyepiece, micro lens, the last recess that is used for placing the smart mobile phone of connecting device and camera hole and the flash light hole that matches with the camera and the flash light of smart mobile phone, the microscope includes micro lens barrel, eyepiece micro lens and objective micro lens, the collapsible one end of micro lens barrel is installed on the push rod of miniature electric motor, the camera hole department on connecting device is installed to miniature electric motor, the remote controller is used for controlling the push rod of miniature electric motor and stretches out and draws back around. The invention combines the smart phone and the handheld microscope, obtains one focusing intensity image and two defocusing intensity images by shooting, solves the phase of the sample by using an intensity transmission equation method, and has the advantages and characteristics of convenient carrying, low cost, rapid imaging and the like.
Description
Technical Field
The invention relates to the technical field of biological microscopic imaging, in particular to an intelligent mobile phone microscopic system based on an intensity transmission equation.
Background
The advent of the 5G era has pushed biomedicine to a deeper level, and doctors can conduct remote video consultation at any time. Among them, the microscopic observation and measurement results are the main basis for disease screening and diagnosis in remote consultation.
Microscopic optical imaging, also known as optical microscopy and optical microscopy imaging, is a technique in which visible light is transmitted through or reflected from a sample, and after passing through one or more lenses, a magnified image of the microscopic sample is obtained. The image can be directly observed through an ocular lens, or can be recorded by a light-sensitive plate and a digital image detector (CCD), and can be displayed and processed on a computer.
In microscopic imaging, most samples are colorless and transparent biological samples, and when light waves are transmitted, intensity information and phase information of the samples are contained in the transmitted light. Studies have shown that about three quarters of the information is stored in phase, while only one quarter of the information is stored in amplitude. The amplitude of the light wave can be directly acquired by the camera, but the phase cannot be directly detected. The loss of phase information necessarily significantly reduces the accuracy of the physician's diagnosis. Therefore, phase estimation (recovery) from the intensity distribution of the sample, i.e., the phase recovery problem, has attracted a great deal of attention.
The methods of phase recovery are mainly divided into two categories: iterative methods and intensity transfer equation methods. Compared with an iterative method, the intensity transmission equation method has great advantages, a complex interference device is not required to be introduced, and the phase distribution of the object can be rapidly and accurately solved through the relation between the intensity and the phase distribution of the object in optical propagation. Meanwhile, the intensity transmission equation method can be well applied to the traditional bright field microscope imaging.
The intensity transfer equation expression is as follows:
wherein the light wave propagates along the z-direction, λ represents the wavelength of the light, I and
respectively represents z0The intensity and phase of the location. In this equation, the partial derivative of the intensity in the z direction is difficult to calculate, and is usually approximated by acquiring multiple intensity images. For example, z can be adopted0+ Δ z and z0The intensity information of the Δ z position is obtained by the following difference calculation formula:
in biological microscopic imaging systems, the data acquisition under home conditions is limited by the disadvantages of conventional commercial electron microscopes, cumbersome equipment and high price. The Chinese patent ZL201110245908.5 provides a portable acquisition reading camera system, which can acquire data in a handheld manner, overcomes the defects of heaviness, difficulty in carrying and the like of the traditional platform-based equipment, and cannot meet the requirement of microscopic imaging in precision, and meanwhile cannot be connected with a smart phone, so that the image cannot be processed immediately.
Disclosure of Invention
The invention aims to provide an intelligent mobile phone microscope system based on an intensity transmission equation.
The technical scheme of the invention is as follows:
a smart phone microscopic system based on an intensity transmission equation comprises a smart phone, a connecting device, a microscope, a miniature electric motor, a remote controller and a support;
the smart phone comprises a camera, a flash lamp and an intensity transmission equation application module, wherein the camera is used for shooting a sample amplified by the microscope, the flash lamp is used for providing a light source during shooting, and the intensity transmission equation application module is used for solving an intensity transmission equation according to an intensity image of the sample obtained by shooting to obtain the phase of the sample;
the connecting device is provided with a groove for placing the smart phone and a camera hole and a flash lamp hole which are matched with a camera and a flash lamp of the smart phone;
the microscope comprises a microscope tube, an eyepiece microlens and an objective microlens, one end of the microscope tube is arranged on a push rod of the miniature electric actuator in a foldable mode, the other end of the microscope tube is a free end, and a movable groove used for placing the eyepiece microlens and a fixed groove used for placing the objective microlens are formed in the microscope tube;
the micro electric actuator is arranged at a camera hole on the connecting device and used for controlling the microscope tube to move back and forth, and a push rod of the micro electric actuator adopts an annular push rod, so that a camera of the smart phone can shoot a sample amplified by the microscope through the micro electric actuator;
the remote controller is used for controlling the push rod of the miniature electric actuator to stretch back and forth, and the support is rotatably arranged on the connecting device.
The smartphone microscope system based on the intensity transmission equation is characterized in that the connecting device is also provided with a clamping groove for accommodating the remote controller.
The smartphone microscope system based on the intensity transmission equation further comprises a patch, wherein the patch is adhered to the connecting device, and the support is rotatably connected with the patch.
According to the smartphone microscope system based on the intensity transmission equation, the connecting device adopts a mobile phone shell.
According to the smartphone microscope system based on the intensity transmission equation, the support is a stretchable support.
According to the technical scheme, the intelligent mobile phone is combined with the handheld microscope, the automatic focusing function of the camera of the intelligent mobile phone is used for shooting the sample amplified by the microscope to obtain a focusing intensity image, the micro-actuator is used for finely adjusting the position of the microscope to obtain two defocusing intensity images, and finally the intensity transmission equation method is used for solving to obtain the phase of the sample.
Drawings
FIG. 1 is an exploded schematic view of the present invention;
FIG. 2 is a schematic view of the structure of the present invention (in use);
fig. 3 is a schematic structural view (stowed state) of the present invention.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
As shown in fig. 1-3, a smartphone microscope system based on an intensity transmission equation includes a smartphone, a connection device 1, a microscope 2, a micro-actuator 3, a remote controller 4, a holder 5, and a patch 6.
The smart phone comprises a camera, a flash lamp and an intensity transmission equation application module, wherein the camera is used for shooting a sample amplified by the microscope 2, the flash lamp is used for providing a light source during shooting, and the intensity transmission equation application module is used for obtaining the phase of the sample by solving an intensity transmission equation according to an intensity image of the sample obtained by shooting.
The connecting device 1 adopts a mobile phone shell, and a groove for placing the smart phone and a camera hole 11 and a flash lamp hole 12 which are matched with a camera and a flash lamp of the smart phone are arranged on the mobile phone shell. The connecting device 1 is used for connecting a smart phone and the microscope 2, and the positions of the camera hole 11 and the flashlight hole 12 are determined according to the model of the smart phone.
The microscope 2 includes a microscope tube 21, an eyepiece microlens 22, and an objective microlens 23. One end of the microscope tube 21 is foldable and mounted on the push rod 31 of the micro-electric actuator 3, and the other end is a free end which is aligned with the sample during shooting. The microscope tube 21 can be folded upside down after use. The microscope tube 21 is hollow and is provided with a movable groove 24 and a fixed groove 25, wherein the movable groove 24 is used for placing the eyepiece microlens 22 and can move back and forth, and the fixed groove 25 is used for placing the objective microlens 23 and cannot move.
The moving distance of the eyepiece microlens 22 depends on the specification of the eyepiece microlens 22 (coarse magnification is determined by the microscope 2, fine magnification is determined by the camera of the smartphone), and the moving distances of eyepiece microlenses 22 of different specifications have marks on the microscope tube 21, and therefore, the position of the movable groove 24 needs to be adjusted according to the specification (focal length) of the eyepiece microlens 22.
The micro-electric device 3 is arranged at the camera hole 11 on the connecting device 1 and is used for controlling the microscope tube 21 to move back and forth, and the micro-scale is controlled by the remote controller 4. The push rod 31 of the miniature electric actuator 3 adopts an annular push rod, so that a camera of the smart phone can shoot a sample amplified by the microscope 2 through the miniature electric actuator 3.
The micro-electric device 3 is controlled by the remote controller 4, the push rod 31 has slow moving speed and micron-sized precision, and the moving distance can be displayed on the remote controller 4, so that the problem that the defocusing distance cannot be determined after focusing is solved.
The remote controller 4 is used for controlling the push rod 31 of the micro-electric actuator 3 to extend and retract back and forth, so that the microscope tube 21 moves back and forth to obtain a defocused image of the sample. The remote control 4 may be received in a card slot provided on the connection device 2.
The moving distance of the push rod 31 is manually set, the moving distance is displayed on a screen of the remote controller 4, and meanwhile, an electric signal generated after the key is pressed is converted into an infrared signal by an infrared transmitting device arranged in the remote controller 4 and is sent to an infrared receiving device of the miniature electric actuator 3.
The micro-electric device 3 uses a small button battery as a power supply, converts the rotary motion of an internal motor into the linear reciprocating motion of the push rod 31, is internally provided with an infrared receiving device, can convert an infrared signal sent by the remote controller 4 into an electric signal after receiving the infrared signal, and then sends the electric signal to an internal CPU (central processing unit) to control the operation of the push rod 31.
The support 5 adopts a stretchable support and can be rotatably arranged on the patch 6, and the patch 6 is stuck on the connecting device 1.
The working process of the invention is as follows:
install the smart mobile phone on connecting device 1, rotate and open microscope tube 21, adorn eyepiece microscope lens 22 and objective microscope lens 23, open the camera of smart mobile phone, the sample is observed roughly, if the magnification is improper, then change eyepiece microscope lens 22 and objective microscope lens 23, adjustment support 5 is placed, and the sample is aimed at to the camera lens of microscope 2, has made the earlier stage preparation work of shooing from this.
And turning on a flash lamp of the smart phone, adjusting the camera magnification of the smart phone, selecting a proper magnification, automatically focusing the sample, and shooting to obtain a focusing intensity image. The remote controller 4 is used to adjust the micro-actuator 3 to move the push rod 31 forward and backward by the same distance (determined by the photographer), and two defocused intensity images are obtained by shooting. And inputting the one focused intensity image and the two defocused intensity images obtained by shooting into an independently developed intensity transmission equation application module, solving an intensity transmission equation, and calculating to obtain the phase of the sample.
In summary, the invention is a portable mobile phone microscope system, the microscope tube 21 is foldable, and the space can be saved when the microscope is not used; because at least two intensity images with different focal lengths are needed when the intensity transmission equation is solved, and the manual focusing of a common camera cannot obtain an accurate defocusing distance, the miniature electric actuator 3 is introduced, the push rod 31 of the miniature electric actuator 3 is accurately moved through the remote controller 4, and the defocusing distance is displayed; by using the support 5, the problem that the shot image is greatly influenced because the smart phone cannot be fixed in the shooting process is solved; the magnification is changed by replacing the eyepiece micro-lenses 22 and the objective micro-lenses 23, different samples can be observed, the positions of the clamping grooves for placing the eyepiece micro-lenses 22 can be adjusted, and the problem of change of the focal lengths of the replaced lenses is solved.
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.
Claims (5)
1. A smart phone microscopic system based on an intensity transmission equation is characterized by comprising a smart phone, a connecting device, a microscope, a miniature electric motor, a remote controller and a support;
the smart phone comprises a camera, a flash lamp and an intensity transmission equation application module, wherein the camera is used for shooting a sample amplified by the microscope, the flash lamp is used for providing a light source during shooting, and the intensity transmission equation application module is used for solving an intensity transmission equation according to an intensity image of the sample obtained by shooting to obtain the phase of the sample;
the connecting device is provided with a groove for placing the smart phone and a camera hole and a flash lamp hole which are matched with a camera and a flash lamp of the smart phone;
the microscope comprises a microscope tube, an eyepiece microlens and an objective microlens, one end of the microscope tube is arranged on a push rod of the miniature electric actuator in a foldable mode, the other end of the microscope tube is a free end, and a movable groove used for placing the eyepiece microlens and a fixed groove used for placing the objective microlens are formed in the microscope tube;
the micro electric actuator is arranged at a camera hole on the connecting device and used for controlling the microscope tube to move back and forth, and a push rod of the micro electric actuator adopts an annular push rod, so that a camera of the smart phone can shoot a sample amplified by the microscope through the micro electric actuator;
the remote controller is used for controlling the push rod of the miniature electric actuator to stretch back and forth, and the support is rotatably arranged on the connecting device.
2. The smartphone microscopy system according to claim 1, wherein: the connecting device is also provided with a clamping groove for accommodating the remote controller.
3. The smartphone microscopy system according to claim 1, wherein: the system further comprises a patch, wherein the patch is pasted on the connecting device, and the support is rotatably connected with the patch.
4. The smartphone microscopy system based on an intensity transmission equation according to claim 1 or 2, wherein: the connecting device adopts a mobile phone shell.
5. The smartphone microscopy system according to claim 1 or 3, wherein the smartphone microscopy system comprises: the support adopts a stretchable support.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011639444.1A CN112799225A (en) | 2020-12-31 | 2020-12-31 | Smart phone microscopic system based on intensity transmission equation |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011639444.1A CN112799225A (en) | 2020-12-31 | 2020-12-31 | Smart phone microscopic system based on intensity transmission equation |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202886724U (en) * | 2012-04-17 | 2013-04-17 | 崔利 | Palm digital microscope based on intelligent handset |
| CN104345438A (en) * | 2014-10-29 | 2015-02-11 | 南京理工大学 | Light intensity transmission phase microscope system based on electronic control zoom lens and method thereof |
| CN104344793A (en) * | 2014-10-29 | 2015-02-11 | 南京理工大学 | Single-frame light intensity transmission quantitative phase microscope system and method |
| CN205507228U (en) * | 2016-03-25 | 2016-08-24 | 温州市鹿城区中津先进科技研究院 | Cellscope |
| WO2017081539A1 (en) * | 2015-11-11 | 2017-05-18 | Scopio Lab Ltd. | Autofocus system for a computational microscope |
| CN111474698A (en) * | 2020-04-30 | 2020-07-31 | 重庆邮电大学 | Double-light-source optical microscopic imaging system and image processing method |
-
2020
- 2020-12-31 CN CN202011639444.1A patent/CN112799225A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202886724U (en) * | 2012-04-17 | 2013-04-17 | 崔利 | Palm digital microscope based on intelligent handset |
| CN104345438A (en) * | 2014-10-29 | 2015-02-11 | 南京理工大学 | Light intensity transmission phase microscope system based on electronic control zoom lens and method thereof |
| CN104344793A (en) * | 2014-10-29 | 2015-02-11 | 南京理工大学 | Single-frame light intensity transmission quantitative phase microscope system and method |
| WO2017081539A1 (en) * | 2015-11-11 | 2017-05-18 | Scopio Lab Ltd. | Autofocus system for a computational microscope |
| CN205507228U (en) * | 2016-03-25 | 2016-08-24 | 温州市鹿城区中津先进科技研究院 | Cellscope |
| CN111474698A (en) * | 2020-04-30 | 2020-07-31 | 重庆邮电大学 | Double-light-source optical microscopic imaging system and image processing method |
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Application publication date: 20210514 |