CN112795592B - OsROC1在水稻育种中的应用 - Google Patents

OsROC1在水稻育种中的应用 Download PDF

Info

Publication number
CN112795592B
CN112795592B CN202110017535.XA CN202110017535A CN112795592B CN 112795592 B CN112795592 B CN 112795592B CN 202110017535 A CN202110017535 A CN 202110017535A CN 112795592 B CN112795592 B CN 112795592B
Authority
CN
China
Prior art keywords
osroc1
rice
plant
plants
breeding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202110017535.XA
Other languages
English (en)
Other versions
CN112795592A (zh
Inventor
魏金环
陈忠科
安镇兴
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nantong University
Original Assignee
Nantong University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nantong University filed Critical Nantong University
Priority to CN202110017535.XA priority Critical patent/CN112795592B/zh
Publication of CN112795592A publication Critical patent/CN112795592A/zh
Application granted granted Critical
Publication of CN112795592B publication Critical patent/CN112795592B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

本发明公开了水稻最外层细胞特异性基因1（OsROC1）在水稻育种中的应用，属于农作物育种技术领域。通过T‑DNA插入构建的OsROC1‑KO植株及RNA干扰技术构建的OsROC1敲降转基因植株，得知OsROC1可调控水稻发育、株高和分蘖等重要农艺性状，为水稻遗传育种强有力的理论依据。

Description

OsROC1在水稻育种中的应用
技术领域
本发明属于农作物育种技术领域，具体涉及水稻最外层细胞特异性基因1（OsROC1）在水稻育种中的应用。
背景技术
近年来，水稻功能基因组研究的快速发展，为我国水稻遗传育种鉴定了大量的有重要利用价值的基因，水稻育种正迈向设计育种的新时代。水稻育种的创新发展为我国口粮安全提供了重要保障，提高单产仍是未来一段时期内我国水稻育种的主攻方向。因此仍要继续重视发掘、克隆和鉴定新的水稻重要农艺性状调控基因，为设计育种提供元件，服务于新时期水稻育种的精准化、数据化和智能化战略需求。
目前，国内外关于同源异型域-亮氨酸拉链蛋白IV家族中的水稻最外层细胞特异性基因亚族的研究并不多，而该亚族基因在水稻中的作用少之又少，本发明对该亚族成员之一OsROC1(Rice outermost cell-specific gene1)的表达及作用进行了研究，初步明确了OsROC1在水稻发育中的作用。
发明内容
本发明的目的是提供了OsROC1在水稻发育中的作用，为水稻育种提供理论基础。
本发明通过构建敲除OsROC1的T-DNA插入突变体和RNAi转基因植株，确定了OsROC1敲除水稻的表型，初步明确了OsROC1在水稻发育中的作用。
OsROC1的核苷酸序列（SEQ ID NO. 1）如下：
AATAAATGCGATTGCATCCGCTCAACACTCGCCCCCCCTCTCGCAATAACTCCTCCTCCCCATCTCCACATTCCTCCCCCAAATCCCCTCTTCTTCTTCTTCTTCTTCTTCCTCCTCCTCCCATGGCGATCGGCGTCTGCTTCTAGCTGGAGAGATCTCGCTTCGTTTCGAGCTGTCCACCGGTGGTTTTCTCGCATGCGATATCACACGCTTCTTGGAAGCACATCAGGTAGAACAGGGCAGAGATCAAGGCGGATATATACAGCTAGATCTCATCTTGTTGCTGTCGTCTCTAACACAGGTTTTGATGGGATTTTTGTTTTTCTTTGCAGAGTTCTTGGTGCTGACCTTGCCCTAGATCTGCTTCTGACTGTTGCTGTTACGTGGCGTGCAAGCTAGCTATAGCCATTTCTGTGTGTGTAGCTGTACTGGTGGTGAAGAAGAAGATGCAAGCGAATGGGTGGAGGAGCTAGAGGCTTGGCTGGAGGAAGAAGAAGAAGGGGTTGGTGGTGGCTAGCTAGCTAGGGTGCGTGCTTAATTACCTGGCTTCGTTCATGACGCCGGCGAGGCGCATGCCGCCGGTGATCGGCCGGAACGGCGTGGCGTACGAATCGCCGTCGGCGCAGCTGCCCCTCACCCAGGTACGGTAGTTCTTCAGAGATGAACAAGAACCCACCGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTTGCAGCTTTCTTTTTTTGAAGTAAATTTTGAAAAAAAGTTGGTGTACTTTTTGTTTGCAGCTAGTTACTTTATTAATTTGCTTTTCGTTTTCTTTTTTCTTGTTGCGTTTGACTCTTGTACTATAATTTAATTGGCTGTGCATCTGTTTTTCATTTTGTTTTGGAAACGGTTGCTAGCTGTGAATACGTGCATGCATTAATTGGCGTGCCATTCGAGGATACTGAGTTTACTGGCCAATTGATGAGCTCTTTTATTGGAAATCAGAAGTACTGGTTGGAGATATCATACACAGTACAGAGAAAGAGAAGTTGATCTTTTGTTCATACAACTTCATACATGAACATGAGTCAAGCAAGAACTGAAGAAACCATACATGAATACATAAACAAAGTCTCTTCCCTTAGGTCTCTCTAGGTCTTGATCTCTTAATGTTCTAATGATCTATGTTTTTTTAAAAAGAAAAAATTTAAAGGCAGACAGGCACAACAGGTTAAGGGAAGGATTAGTTCATTTTTAGCATCATACTAAAAAGAAAAATAGCTAGCTATATCCATTTTTGACAATCACGTCTCGAAAATACACTATACTGAGCAGAAGCAAGTCAGCAATTTCCAACTTTGCGGCCGAAGTAACTACTCTATCCTATCCCTTTTAGAAAGAAAAAGAAATGGAAGCATGCAAAATTTCCCCTTTTTTATGAGCGTTTCGTATAAGTTTTTCTTTGGTACATCTTAATTGCTTTCTCATTCGATTATTCCAAATTAATCCTGAGCTAGCTATATATGGAACACATATATCTGATGAGTTGGTCTCCTCTCACAAAATACTCTTAATTAATCTCTCCTGAAATTGCAGGCTGATATGCTGGACAGCCATCATCTGCAGCAAGCACTCCAGCAGCAATACTTCGATCAGATCCCGGTGACGACGACGGCGGCGGCGGACAGCGGCGACAACATGCTGCACGGCCGCGCCGACGCCGGCGGGCTGGTTGACGAGTTCGAGAGCAAGTCGTGCAGCGAGAACGTCGACGGCGCCGGCGACGGCCTCTCCGGCGACGACCAGGACCCCAACCAGCGGCCGCGCAAGAAGCGTTACCACCGCCATACCCAGCACCAGATCCAAGAGATGGAAGCGTAAGATTAATTTATCATTTCATCCCTATGTTTTCAATTCTGAGATAATTTTCTTCTCCCTTGCAAATCTTTGTCAGATTGATTAAATGTTATGAATCCTATTCATCAGTTGATCTTTGTAGATTAAGTAAATGTTTGTGAATTTTGATCAGTTTCTTCAAGGAGTGCCCGCACCCAGACGACAAGCAGCGCAAGGAGCTGAGCAGGGAGCTGGGTCTTGAACCTCTGCAGGTTAAATTCTGGTTTCAGAACAAGCGCACACAGATGAAGGCAAGTATAAAAACTCCTTATAGATATATAGAGTGTTCTTTGGTTATTTAATTTGATGGCTTCAGACTAAAATGATTGGGAATTACTGAAAAATAAAATTTTGGTGCACTAAATTCCAAAATCCAAGACTAGGAAAAAAAAACACGTTGGACACTGGGGAAGGACCCAATATCGAATTTATTAAGAAAGAGGGTCAACCAGCTGCACCATGCCATCAGCGCTACACACAGTCACAAAATCCAAGACTAAGAAACGGATTATTGCAATCCATGTTCTTTTCAGTTCATTCACAATATTATTCAAATAGATTATTTATTCCATGCTTTAAAAAATATGTTTTTCTCAAAACAATTTCTCAAAAATTACTGCCTATTTATTTCTTAAAACATTTAATATAAACCTTATAATTAAGTAGATGTTCCGCGACAATAATCTAAATCAAATAGGGGTGAGTTCTAAATCTAAATCAGCTAATAATTTGATTTTTCATTCTTCATACTAGTACAATAATGAGCAGTTTTCTACTTAATTACTGGAGCATGTATAATATTTATATACTTAAGTTACAGTATCTATATGAATATTTAGATTGAACAACGAAGGTATATAATGTTTATATAACTATCTCCATATATCTTTCATGATACTAAATCTTATAGTATCTTTCAGAATTTATATATTCTAATACAAACTATAGTCATCAAAGTTGTGTTTTCTCTAAACCATATTCCAATGCACTTAATTGTTTGTGACCGGAGTTAATTAGTACTGCTATCAATTTTTGGCCAAAAAAGCAAATTAGGTTGTGCACACACGGATGAAACTAGGCGGTACTACTAAGAAAGGACGATGTTTTTTTTTTTTGGGGGGGGGGGGTCTTGAATTAAAATGTTTGGATAACACTGAGGAAAAGTTTTCTCAACTTGAAATCAATGCAAGTGTCTAAACCTCACATAACAATTTGCACTGGCTTGCATTAACCAATTTTCCCGAGGACGCGGATGTTGCTCAGCAGCAAGATTGTTTCCTGCACCACCACAGCAGTTAGTGTTTGAATTGAACCTTGTTGTATCTCTTAATGGAACAGACTATATGAAACAACATTGGGACTCACCATTTAGTTTTAGTTTATGGTTAATTACTATGAAGAAATACTCCATTCGTCTTAAAATATTAGTATTAGTAGAATAATGCTAAGTCAAACATTTTCAGCTTTATCTATTAATAGTCAAAAAAAATAAAACAGATCAATCATTGTAAAATTGATGTTACTAGATTTATCATTAAATAAATTATCATAATATATAACTCTTTTTATTTAAAGCATCTTAACTTTATAGATATTATTGGTTAAATGAGCATCTCGAAAATTGTGTTAAAGTTCAAAAATGCTTATATTTTGGAACGGAGAGAGCATGTTATTTCTTTCTTCACTTCTTTTTTTCATTTCTGATTTATTTTATTTCACTTCTCCCTCTGGCCTCTACTCCTGTTATACATGGTAGATATAGTGAAATATATTCCAGCCCAGACGTGTGTGTGTGTATATATATATGCATCTTTTTGTCTATTGAACAGATGGAAAAATATTGTGTTTTCTGTCAGCTACATATATTGATCGATTGACCCTTACAAAACTATAAATATATCAGTAAAATTAATGTTGTAATAGTATATTGACGATATGTAGTGTTGAATTAATTCAGAACCAGCACGAGAGGCACGAGAACGCGCAGCTGCGGGCGGAGAACGACAAGCTGCGCGCGGAGAACATGCGATACAAGGAGGCCCTGAGCAGCGCGTCGTGCCCCAACTGCGGCGGCCCCGCCGCCCTCGGCGAGATGTCCTTCGACGAGCACCACCTCCGCGTCGAGAACGCCCGCCTCCGCGACGAGATCGACCGCATCTCCGGCATCGCCGCCAAGCACGTCGGCAAGCCCCCCATCGTCTCCTTCCCCGTCCTCTCCTCCCCGCTCGCCGTCGCCGCCGCCCGCTCCCCTCTCGACCTCGCCGGCGCCTACGGCGTCGTCACCCCCGGCCTCGACATGTTCGGCGGCGCCGGCGACCTCCTCCGCGGCGTGCACCCGCTCGACGCCGACAAGCCCATGATCGTGGAGCTCGCCGTCGCCGCCATGGACGAGCTCGTCCAGATGGCCCAGCTCGACGAGCCGCTCTGGTCGTCGTCGTCGGAGCCGGCGGCGGCGTTGCTCGATGAGGAGGAGTACGCGCGCATGTTCCCGCGCGGCCTCGGCCCCAAGCAGTACGGCCTCAAGTCGGAGGCGTCCCGCCATGGCGCCGTCGTCATCATGACGCACAGCAACCTCGTCGAGATCCTCATGGATGTGGTCAGTCTCACAATACTCAAACCTACTACTGCTAGTGCTACCTAAATATGCAGTAATTTCAGTTGAATTTCGCTTGAATAATTTTGCTGCAGAATCAATTCGCGACGGTGTTCTCGAGCATCGTGTCGAGAGCGTCCACGCACGAGGTGTTGTCCACAGGCGTGGCAGGCAACTACAATGGTGCACTGCAAGTGGTACGTACACGTCTTCTTCATCCTCATCGACTACTCGATCAACTCCTTAATTAATCTGTTCATGGCACGCCTCCAATTCCATGCTCCATGGATATATTCTGGAATTAATCCACCAGATATATGATGATCTAAACATTATCGGTTGCAGATGTCAATGGAGTTTCAAGTGCCGTCGCCGCTGGTGCCGACGAGGGAGAGCTATTTCGTCAGGTACTGCAAGAACAACTCCGACGGGACATGGGCCGTCGTCGATGTCTCTCTCGACAGCCTCCGCCCCAGCCCTGTCCAGAAATGCCGGCGCAGGCCGTCTGGCTGCCTCATCCAAGAAATGCCCAATGGCTACTCCAAGGTAATTAATTCAATCTCTATATATATATATCTACAATATTAAAGGATAGTACATATTGTTGTTGTTCATATTTTGTCAGCAAAAGTTGAAATGGTTTGATCGTTCATATTCTGTTGGCAAAGTTAATTTGAAATGTATTGGGTTGTTGTGTGTAGGTGACATGGGTGGAGCATGTGGAGGTGGACGACAGCTCGGTGCACAACATCTACAAGCCATTGGTGAACTCCGGCCTCGCATTCGGCGCGAAACGGTGGGTCGGCACGCTGGACCGGCAATGCGAGCGCCTCGCCAGCGCCATGGCCAGCAACATCCCCAATGGCGACCTTGGAGGTACAAGACGAATCAATCAATCAATTAACCACTCCTACGTCCTACTAATTGCAATTATAAGAAGTACAGATAATTGTGACCAATGAAATGCCACATTACAATCATGATTACTGATGATTAGTATAATTTGTGTATGAAATGTTATGTGTTGTGGTGCAGTGATTACAAGCGTGGAGGGGAGGAAGAGCATGTTGAAGCTGGCGGAGAGGATGGTGGCGAGCTTCTGCGGCGGCGTGACGGCGTCGGTGGCGCACCAGTGGACGACGCTGTCGGGCAGCGGGGCGGAGGACGTGCGCGTCATGACGAGGAAGAGCGTCGACGACCCCGGCAGGCCACCGGGCATCGTGCTCAACGCCGCCACCTCCTTCTGGCTCCCCGTCCCTCCCGCCGCCGTCTTCGACTTCCTCCGCGACGAGACCTCTCGCAGCGAGGTGCCCAATTTGCCCCCCATCCCTAGGGAATTTATCCAATTTGCTGACCAAATTTTATACGTGATCAAATTCATTCCTGACGAGTTTGGATTTCCGCAGTGGGACATTCTGTCCAACGGCGGCGCCGTCCAAGAAATGGCTCACATTGCCAACGGCCGTGACCATGGCAACTCTGTCTCGCTTCTTCGTGTCAATGTAAGTGCCAATTAGTTACTACTCATCAAGGCAATAAATAGCATGTAACGGTCGGTAGCGAATTAGTGTCTGGGTAGCGGATTGCAAATAGCAGGCACTAAGTTCCAATAGCATAATTAAGAAAAATAACCATAAATTTAGATTGTATATCAATATTAACATCAAGTTTCACTTAAAGTTTAAAATTTAGACTGCATGTTTAACTACTACTACATTTCATAACATAAATATATAAGATAAAGAAAACATACAATGCTAGGTTATTCTATCCATATGTAAAGAACACTATCTAAACATTATAGCAATTCAACAAAGTCAATAGCAGGCTGCTATACCTTGTAGCGGCATCGGTAGAGGATCACTACTGCTATATTGCAGCCGCTACGAATGCTATTTATTACCTTCTGCTCATGAATCTGATGATTACACTGTTTACACACGATGAAATCGGGTATCATCATTTCTATAAAATTAAGTAATGATGTGTTTATATATGTATGATTTGTCCATGTGTGTAGAGTGCAAATTCAAACCAGAGCAACATGCTGATCCTGCAAGAGAGCTGCACGGACGCGTCGGGCTCCTACGTGGTGTACGCGCCGGTGGACATCGTGGCGATGAACGTGGTGCTCAACGGCGGCGACCCGGACTACGTGGCGCTGCTGCCGTCGGGGTTCGCCATCCTTCCCGACGGGCCGTCGGGGAACGCGCAGGCCGCCGTCGGGGAGAACGGCTCCGGCTCCGGCGGGGGGTCCCTCCTGACGGTGGCGTTCCAGATCCTCGTCGACTCCGTGCCGACGGCGAAGCTCTCGCTGGGCTCCGTCGCGACGGTGAACAGCCTCATCGCCTGCACGGTGGAGCGCATCAAGGCCGCCGTCTGCAGGGACAGCAACCCTCAGTAGTAGCACCGGCTTCAACCAAATATGATCACGAACAATGTTCCAAGGTACATGCTTGCTACGTTTAGTAAAGCGTTGCTGCACGAATCTCAAAGCACCTACTGAAATGTTTTGAATTTGTGTGTGTGTGTTTTTTTGCAGGAGCAGCAAAATTTTGGAGTTTGATTCAGCAGTAGGGAGTCAAGAACGCACCTCAAACTCTCCTTGCCATGTGGTGCCCTTGCTAGTGAAGAATTTGCGAGGAAACCGACGCGTTTGCGAGAAAATTTTCGCGAAGATACATAGTGTCACCACTATGCAAGGGGGGATGGTTCGGGCATTGACTTCCACCTTTTGATTTTCATCAAATGCATCGAAGAGTGGATGCTGCTACCTTGTTGTTTTTCTGAATCTCTATGTTAGGGCTTCTTTTACCATGTTTAAAAAGGGCTATGAAGCCTCATTTTTATCTGTACTGTTGTGATGTGTTGTCTGTTTGTTCAGACTTCAGGTTCCTGTCCCTTAGCTAGGAGTTGAGAATAGAGAGATTCATGTCCCCCCTTTTGGATTTTATCACATTTCCGATGATTTGTAGACTTGTGGTTGTTGCTTGTTGGTCATTACCATTCTTGTCTCAAA
通过T-DNA插入构建的OsROC1-KO植株及RNA干扰技术构建的OsROC1敲降转基因植株的表型一致，与野生型植株相比，OsROC1-KO植株及OsROC1敲降转基因植株都呈现发育减缓、株高降低、分蘖数减少的表型。进一步的实验表明，OsROC1-KO植株中的细胞大小与野生型植株相比明显减小，推测细胞减小是OsROC1-KO植株呈现发育减缓、株高降低、分蘖数减少表型的主要原因。
附图说明
图1为OsROC1的T-DNA插入突变体基因型分选。A为T-DNA插入位点、RNA干扰及引物位置；B为OsROC1的T-DNA插入突变体基因型分选，WT：野生型，KO：OsROC1敲除纯合子植株；C为OsROC1在野生型（WT）和纯合子（HO）植株中的表达。
图2为野生型植株和OsROC1敲除纯合子植株在不同时期的表型。A为种子发芽后2天；B为种子发芽后5天；C为种子发芽后33天；D为种子发芽后144天；E为收种时期；F为植株高度；G为有效分蘖数。其中野生型植株和OsROC1敲除纯合子植株的统计数量分别大于15株。
图3为OsROC1-RNAi转基因植株表型。A为第一代OsROC1-RNAi转基因植株中OsROC1的表达量；B和C分别为OsROC1-RNAi转基因植株4#和6#子代植株的表型；D为OsROC1-RNAi转基因植株4#和6#子代植株的株高。
图4为各节间长度及第二节节间细胞形态大小。A为各节间长度；B为第二节节间细胞形态大小。其中，野生型及OsROC1-KO植株数多于6。
具体实施方式
下面结合附图通过实施例来对本发明进行详细说明，但并不是对本发明的限制，仅仅作示例说明。
实施例1
1．敲除载体的构建和敲除水稻株系的获得
在Oryza sativa cv. Dongjin中构建OsROC1的T-DNA插入突变体种系2C-50198（［1］Jinhuan Wei, Heebak Choi, Ping Jin, Yunfei Wu, Jinmi Yoon, Yang-Seok Lee,Taiyong Quan, Gynheung An; GL2-type homeobox gene Roc4 in rice promotesflowering time preferentially under long days by repressing Ghd7, PlantScience, 2016, 252: 133–143［2］Jinhuan Wei, Yunfei Wu, Lae-Hyeon Cho, JinmiYoon, Heebak Choi, Ping Jin, Jakyung Yi, Yang-Seok Lee, Hee Joong Jeong,Jungil Yang, Gynheung An; Identification of root-preferential transcriptionfactors in rice by analyzing GUS expression patterns of T-DNA tagging lines,Journal of Plant Biology, 2017, 60 (3): 268-277）。
植物种植在大田或温室里（14.5小时光照，28℃/9.5小时黑暗，22℃，湿度50%）。种子用含有3%蔗糖的MS培养基（Murashig and Skoog Medium）发芽，种子发芽7天后将幼苗移植到土壤中。
T-DNA插入纯合子突变体植物用以下的引物进行基因型鉴定：
F1：5'-AGGAAGAGCATGTTGAAGCT-3'（SEQ ID NO. 2），
R1：5'-CTACTGAGGGTTGCTGTCCCT-3'（SEQ ID NO. 3）
和5'-AACGCTGATCAATTCCACAG-3' (NGUS1) （SEQ ID NO. 4）。
如图1所示，株系2C-50198中OsROC1被敲除。
2. OsROC1敲除株系的RNA提取和实时定量PCR分析
提取最上面第二片叶子的中间1.5到2厘米部分的RNA。总RNA提取使用RNAisoPlus（TaKaRa, Shiga, Japan），保证RNA样品260/280的比大于1.8 （NanoDrop 2000;Thermo Scientific, Wilmington, DE, USA）。cDNA合成采用2μg 总RNA、Moloney murineleukemia virus 逆转录酶（Promega, Madison, WI, USA）、RNasin® RibonucleaseInhibitor（Promega）、oligo (dT)18 引物和dNTP。实时定量PCR检测目的基因表达采用SYBR Green I Prime Q-Master mix （GENETBIO, Daejeon, Republic of Korea）和theRotor-Gene Q（QIAGEN, Hilden, Germany）。所有实验至少取样自四棵不同植株，重复三次以上。基因表达变化根据∆∆Ct方法计算。引物的特异性根据熔点曲线确定，OsUbi5作为内参。
OsUbi5引物序列如下：
F：5'-TGAAGACCCTGACTGGGAAG-3'（SEQ ID NO. 5）
R：5'-CACGGTTCAACAACATCCAG -3'（SEQ ID NO. 6）。
OsROC1的qRT-PCR引物如下：
F2：5'-GGTTCAGGGGGTAATTTGTA-3'（SEQ ID NO. 7）
R2：5'-ATGTGAGCCATTTCTTGGAC-3'（SEQ ID NO. 8）。
如图2所示，与野生型植株相比，OsROC1敲除纯合子植株从发芽时期开始呈现发育迟缓的表型（图2：A-B），从分蘖期到结实期的分蘖数显著低于野生型（图2：C-E）。收种期统计株高发现，野生型植株平均株高169.5cm，而OsROC1敲除纯合子植株株高只有136.6cm，降低了20%（图2：F），野生型植株平均有效分蘖数为13，而OsROC1敲除纯合子植株有效分蘖数只有4个（图2：G）。
3. RNA干扰载体构建和水稻转化
RNAi载体构建引物如下：
RNAi-F：5'-AGATCCCGGTGACGACGACG-3'（SEQ ID NO. 9）
RNAi-R：5'-CAGCGCGTCGTGCCC GGTACC CC-3'（SEQ ID NO. 10）
划线部分为KpnI酶切位点。
使用Pfu高保真酶进行PCR，产物使用乙醇纯化后，用KpnI消化，载体pGA3720使用SmaI消化，载体pGA3426使用KpnI消化，将三种产物连接后，进行大肠杆菌质粒转化。根据冻融方法将RNAi载体转染到农杆菌Agrobacterium tumefaciens LBA4404中。转基因植物通过农杆菌转化方法培育。
如图3所示，通过RNA干扰技术构建了OsROC1-RNAi转基因植株，在第一代的22株植物中，通过检测OsROC1的表达量发现1#、2#、4#、5#、9#、10#、13#、14#、16、18#、19#中的变化显著，说明OsROC1在这些转基因植株中被敲降。在下一代的培育中，随机选取4#和6#的种子。温室种植到结实期发现6#子代的株高与野生型植株株高基本一致（85cm左右），而4#子代的株高只有67cm，比野生型植株降低了20%，该结果与OsROC1敲除纯合子植株的表型一致。
4. 石蜡组织切片及染色
为进一步揭示OsROC1的作用，我们对温室中每棵植株的最长茎秆的节间进行了测量。取水稻植株最长茎秆上的第二节节间0.5cm茎秆，用FAA固定液固定24小时以上，经脱水、脱酒精、石蜡包埋后，进行切片，厚度8微米，脱蜡后用番红-固绿染色，封片后用BX61光学显微镜拍照。
对温室中每棵植株的最长茎秆的节间进行了测量结果显示野生型与OsROC1-KO植株第2个节间的长度差异最为显著（图4A所示）。对第二节节间进行的石蜡切片及番红-固绿染色，发现OsROC1-KO植株中的细胞大小明显减小（图4B所示）。
由以上实验可知，通过T-DNA插入构建的OsROC1-KO植株及RNA干扰技术构建的OsROC1敲降转基因植株的表型一致，与野生型植株相比，OsROC1-KO植株及OsROC1敲降转基因植株都呈现发育减缓、株高降低、分蘖数减少的表型。进一步的实验表明，OsROC1-KO植株中的细胞大小与野生型植株相比明显减小，推测细胞减小是OsROC1-KO植株呈现发育减缓、株高降低、分蘖数减少表型的主要原因。本发明的发现将为水稻育种提供强有力的理论依据。
序列表
<110> 南通大学
<120> OsROC1在水稻育种中的应用
<130> 20210106
<160> 10
<170> SIPOSequenceListing 1.0
<210> 1
<211> 7544
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
aataaatgcg attgcatccg ctcaacactc gccccccctc tcgcaataac tcctcctccc 60
catctccaca ttcctccccc aaatcccctc ttcttcttct tcttcttctt cctcctcctc 120
ccatggcgat cggcgtctgc ttctagctgg agagatctcg cttcgtttcg agctgtccac 180
cggtggtttt ctcgcatgcg atatcacacg cttcttggaa gcacatcagg tagaacaggg 240
cagagatcaa ggcggatata tacagctaga tctcatcttg ttgctgtcgt ctctaacaca 300
ggttttgatg ggatttttgt ttttctttgc agagttcttg gtgctgacct tgccctagat 360
ctgcttctga ctgttgctgt tacgtggcgt gcaagctagc tatagccatt tctgtgtgtg 420
tagctgtact ggtggtgaag aagaagatgc aagcgaatgg gtggaggagc tagaggcttg 480
gctggaggaa gaagaagaag gggttggtgg tggctagcta gctagggtgc gtgcttaatt 540
acctggcttc gttcatgacg ccggcgaggc gcatgccgcc ggtgatcggc cggaacggcg 600
tggcgtacga atcgccgtcg gcgcagctgc ccctcaccca ggtacggtag ttcttcagag 660
atgaacaaga acccaccgta tatatatata tatatatata tatatatata tatatatata 720
tatatatata tatatatata tatatatata tatatatata tatatatata tatatttgca 780
gctttctttt tttgaagtaa attttgaaaa aaagttggtg tactttttgt ttgcagctag 840
ttactttatt aatttgcttt tcgttttctt ttttcttgtt gcgtttgact cttgtactat 900
aatttaattg gctgtgcatc tgtttttcat tttgttttgg aaacggttgc tagctgtgaa 960
tacgtgcatg cattaattgg cgtgccattc gaggatactg agtttactgg ccaattgatg 1020
agctctttta ttggaaatca gaagtactgg ttggagatat catacacagt acagagaaag 1080
agaagttgat cttttgttca tacaacttca tacatgaaca tgagtcaagc aagaactgaa 1140
gaaaccatac atgaatacat aaacaaagtc tcttccctta ggtctctcta ggtcttgatc 1200
tcttaatgtt ctaatgatct atgttttttt aaaaagaaaa aatttaaagg cagacaggca 1260
caacaggtta agggaaggat tagttcattt ttagcatcat actaaaaaga aaaatagcta 1320
gctatatcca tttttgacaa tcacgtctcg aaaatacact atactgagca gaagcaagtc 1380
agcaatttcc aactttgcgg ccgaagtaac tactctatcc tatccctttt agaaagaaaa 1440
agaaatggaa gcatgcaaaa tttccccttt tttatgagcg tttcgtataa gtttttcttt 1500
ggtacatctt aattgctttc tcattcgatt attccaaatt aatcctgagc tagctatata 1560
tggaacacat atatctgatg agttggtctc ctctcacaaa atactcttaa ttaatctctc 1620
ctgaaattgc aggctgatat gctggacagc catcatctgc agcaagcact ccagcagcaa 1680
tacttcgatc agatcccggt gacgacgacg gcggcggcgg acagcggcga caacatgctg 1740
cacggccgcg ccgacgccgg cgggctggtt gacgagttcg agagcaagtc gtgcagcgag 1800
aacgtcgacg gcgccggcga cggcctctcc ggcgacgacc aggaccccaa ccagcggccg 1860
cgcaagaagc gttaccaccg ccatacccag caccagatcc aagagatgga agcgtaagat 1920
taatttatca tttcatccct atgttttcaa ttctgagata attttcttct cccttgcaaa 1980
tctttgtcag attgattaaa tgttatgaat cctattcatc agttgatctt tgtagattaa 2040
gtaaatgttt gtgaattttg atcagtttct tcaaggagtg cccgcaccca gacgacaagc 2100
agcgcaagga gctgagcagg gagctgggtc ttgaacctct gcaggttaaa ttctggtttc 2160
agaacaagcg cacacagatg aaggcaagta taaaaactcc ttatagatat atagagtgtt 2220
ctttggttat ttaatttgat ggcttcagac taaaatgatt gggaattact gaaaaataaa 2280
attttggtgc actaaattcc aaaatccaag actaggaaaa aaaaacacgt tggacactgg 2340
ggaaggaccc aatatcgaat ttattaagaa agagggtcaa ccagctgcac catgccatca 2400
gcgctacaca cagtcacaaa atccaagact aagaaacgga ttattgcaat ccatgttctt 2460
ttcagttcat tcacaatatt attcaaatag attatttatt ccatgcttta aaaaatatgt 2520
ttttctcaaa acaatttctc aaaaattact gcctatttat ttcttaaaac atttaatata 2580
aaccttataa ttaagtagat gttccgcgac aataatctaa atcaaatagg ggtgagttct 2640
aaatctaaat cagctaataa tttgattttt cattcttcat actagtacaa taatgagcag 2700
ttttctactt aattactgga gcatgtataa tatttatata cttaagttac agtatctata 2760
tgaatattta gattgaacaa cgaaggtata taatgtttat ataactatct ccatatatct 2820
ttcatgatac taaatcttat agtatctttc agaatttata tattctaata caaactatag 2880
tcatcaaagt tgtgttttct ctaaaccata ttccaatgca cttaattgtt tgtgaccgga 2940
gttaattagt actgctatca atttttggcc aaaaaagcaa attaggttgt gcacacacgg 3000
atgaaactag gcggtactac taagaaagga cgatgttttt tttttttggg gggggggggt 3060
cttgaattaa aatgtttgga taacactgag gaaaagtttt ctcaacttga aatcaatgca 3120
agtgtctaaa cctcacataa caatttgcac tggcttgcat taaccaattt tcccgaggac 3180
gcggatgttg ctcagcagca agattgtttc ctgcaccacc acagcagtta gtgtttgaat 3240
tgaaccttgt tgtatctctt aatggaacag actatatgaa acaacattgg gactcaccat 3300
ttagttttag tttatggtta attactatga agaaatactc cattcgtctt aaaatattag 3360
tattagtaga ataatgctaa gtcaaacatt ttcagcttta tctattaata gtcaaaaaaa 3420
ataaaacaga tcaatcattg taaaattgat gttactagat ttatcattaa ataaattatc 3480
ataatatata actcttttta tttaaagcat cttaacttta tagatattat tggttaaatg 3540
agcatctcga aaattgtgtt aaagttcaaa aatgcttata ttttggaacg gagagagcat 3600
gttatttctt tcttcacttc tttttttcat ttctgattta ttttatttca cttctccctc 3660
tggcctctac tcctgttata catggtagat atagtgaaat atattccagc ccagacgtgt 3720
gtgtgtgtat atatatatgc atctttttgt ctattgaaca gatggaaaaa tattgtgttt 3780
tctgtcagct acatatattg atcgattgac ccttacaaaa ctataaatat atcagtaaaa 3840
ttaatgttgt aatagtatat tgacgatatg tagtgttgaa ttaattcaga accagcacga 3900
gaggcacgag aacgcgcagc tgcgggcgga gaacgacaag ctgcgcgcgg agaacatgcg 3960
atacaaggag gccctgagca gcgcgtcgtg ccccaactgc ggcggccccg ccgccctcgg 4020
cgagatgtcc ttcgacgagc accacctccg cgtcgagaac gcccgcctcc gcgacgagat 4080
cgaccgcatc tccggcatcg ccgccaagca cgtcggcaag ccccccatcg tctccttccc 4140
cgtcctctcc tccccgctcg ccgtcgccgc cgcccgctcc cctctcgacc tcgccggcgc 4200
ctacggcgtc gtcacccccg gcctcgacat gttcggcggc gccggcgacc tcctccgcgg 4260
cgtgcacccg ctcgacgccg acaagcccat gatcgtggag ctcgccgtcg ccgccatgga 4320
cgagctcgtc cagatggccc agctcgacga gccgctctgg tcgtcgtcgt cggagccggc 4380
ggcggcgttg ctcgatgagg aggagtacgc gcgcatgttc ccgcgcggcc tcggccccaa 4440
gcagtacggc ctcaagtcgg aggcgtcccg ccatggcgcc gtcgtcatca tgacgcacag 4500
caacctcgtc gagatcctca tggatgtggt cagtctcaca atactcaaac ctactactgc 4560
tagtgctacc taaatatgca gtaatttcag ttgaatttcg cttgaataat tttgctgcag 4620
aatcaattcg cgacggtgtt ctcgagcatc gtgtcgagag cgtccacgca cgaggtgttg 4680
tccacaggcg tggcaggcaa ctacaatggt gcactgcaag tggtacgtac acgtcttctt 4740
catcctcatc gactactcga tcaactcctt aattaatctg ttcatggcac gcctccaatt 4800
ccatgctcca tggatatatt ctggaattaa tccaccagat atatgatgat ctaaacatta 4860
tcggttgcag atgtcaatgg agtttcaagt gccgtcgccg ctggtgccga cgagggagag 4920
ctatttcgtc aggtactgca agaacaactc cgacgggaca tgggccgtcg tcgatgtctc 4980
tctcgacagc ctccgcccca gccctgtcca gaaatgccgg cgcaggccgt ctggctgcct 5040
catccaagaa atgcccaatg gctactccaa ggtaattaat tcaatctcta tatatatata 5100
tctacaatat taaaggatag tacatattgt tgttgttcat attttgtcag caaaagttga 5160
aatggtttga tcgttcatat tctgttggca aagttaattt gaaatgtatt gggttgttgt 5220
gtgtaggtga catgggtgga gcatgtggag gtggacgaca gctcggtgca caacatctac 5280
aagccattgg tgaactccgg cctcgcattc ggcgcgaaac ggtgggtcgg cacgctggac 5340
cggcaatgcg agcgcctcgc cagcgccatg gccagcaaca tccccaatgg cgaccttgga 5400
ggtacaagac gaatcaatca atcaattaac cactcctacg tcctactaat tgcaattata 5460
agaagtacag ataattgtga ccaatgaaat gccacattac aatcatgatt actgatgatt 5520
agtataattt gtgtatgaaa tgttatgtgt tgtggtgcag tgattacaag cgtggagggg 5580
aggaagagca tgttgaagct ggcggagagg atggtggcga gcttctgcgg cggcgtgacg 5640
gcgtcggtgg cgcaccagtg gacgacgctg tcgggcagcg gggcggagga cgtgcgcgtc 5700
atgacgagga agagcgtcga cgaccccggc aggccaccgg gcatcgtgct caacgccgcc 5760
acctccttct ggctccccgt ccctcccgcc gccgtcttcg acttcctccg cgacgagacc 5820
tctcgcagcg aggtgcccaa tttgcccccc atccctaggg aatttatcca atttgctgac 5880
caaattttat acgtgatcaa attcattcct gacgagtttg gatttccgca gtgggacatt 5940
ctgtccaacg gcggcgccgt ccaagaaatg gctcacattg ccaacggccg tgaccatggc 6000
aactctgtct cgcttcttcg tgtcaatgta agtgccaatt agttactact catcaaggca 6060
ataaatagca tgtaacggtc ggtagcgaat tagtgtctgg gtagcggatt gcaaatagca 6120
ggcactaagt tccaatagca taattaagaa aaataaccat aaatttagat tgtatatcaa 6180
tattaacatc aagtttcact taaagtttaa aatttagact gcatgtttaa ctactactac 6240
atttcataac ataaatatat aagataaaga aaacatacaa tgctaggtta ttctatccat 6300
atgtaaagaa cactatctaa acattatagc aattcaacaa agtcaatagc aggctgctat 6360
accttgtagc ggcatcggta gaggatcact actgctatat tgcagccgct acgaatgcta 6420
tttattacct tctgctcatg aatctgatga ttacactgtt tacacacgat gaaatcgggt 6480
atcatcattt ctataaaatt aagtaatgat gtgtttatat atgtatgatt tgtccatgtg 6540
tgtagagtgc aaattcaaac cagagcaaca tgctgatcct gcaagagagc tgcacggacg 6600
cgtcgggctc ctacgtggtg tacgcgccgg tggacatcgt ggcgatgaac gtggtgctca 6660
acggcggcga cccggactac gtggcgctgc tgccgtcggg gttcgccatc cttcccgacg 6720
ggccgtcggg gaacgcgcag gccgccgtcg gggagaacgg ctccggctcc ggcggggggt 6780
ccctcctgac ggtggcgttc cagatcctcg tcgactccgt gccgacggcg aagctctcgc 6840
tgggctccgt cgcgacggtg aacagcctca tcgcctgcac ggtggagcgc atcaaggccg 6900
ccgtctgcag ggacagcaac cctcagtagt agcaccggct tcaaccaaat atgatcacga 6960
acaatgttcc aaggtacatg cttgctacgt ttagtaaagc gttgctgcac gaatctcaaa 7020
gcacctactg aaatgttttg aatttgtgtg tgtgtgtttt tttgcaggag cagcaaaatt 7080
ttggagtttg attcagcagt agggagtcaa gaacgcacct caaactctcc ttgccatgtg 7140
gtgcccttgc tagtgaagaa tttgcgagga aaccgacgcg tttgcgagaa aattttcgcg 7200
aagatacata gtgtcaccac tatgcaaggg gggatggttc gggcattgac ttccaccttt 7260
tgattttcat caaatgcatc gaagagtgga tgctgctacc ttgttgtttt tctgaatctc 7320
tatgttaggg cttcttttac catgtttaaa aagggctatg aagcctcatt tttatctgta 7380
ctgttgtgat gtgttgtctg tttgttcaga cttcaggttc ctgtccctta gctaggagtt 7440
gagaatagag agattcatgt cccccctttt ggattttatc acatttccga tgatttgtag 7500
acttgtggtt gttgcttgtt ggtcattacc attcttgtct caaa 7544
<210> 2
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
aggaagagca tgttgaagct 20
<210> 3
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
ctactgaggg ttgctgtccc t 21
<210> 4
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
aacgctgatc aattccacag 20
<210> 5
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
tgaagaccct gactgggaag 20
<210> 6
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 6
cacggttcaa caacatccag 20
<210> 7
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
ggttcagggg gtaatttgta 20
<210> 8
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
atgtgagcca tttcttggac 20
<210> 9
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
agatcccggt gacgacgacg 20
<210> 10
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 10
cagcgcgtcg tgcccggtac ccc 23

Claims (1)

1.OsROC1在水稻育种中的应用，其特征在于：OsROC1用于促进水稻发育、OsROC1用于调控水稻株高、OsROC1用于调控水稻分蘖；
所述OsROC1的核苷酸序列如SEQ ID NO. 1所示。
CN202110017535.XA 2021-01-07 2021-01-07 OsROC1在水稻育种中的应用 Active CN112795592B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110017535.XA CN112795592B (zh) 2021-01-07 2021-01-07 OsROC1在水稻育种中的应用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110017535.XA CN112795592B (zh) 2021-01-07 2021-01-07 OsROC1在水稻育种中的应用

Publications (2)

Publication Number Publication Date
CN112795592A CN112795592A (zh) 2021-05-14
CN112795592B true CN112795592B (zh) 2021-09-14

Family

ID=75808877

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110017535.XA Active CN112795592B (zh) 2021-01-07 2021-01-07 OsROC1在水稻育种中的应用

Country Status (1)

Country Link
CN (1) CN112795592B (zh)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107326033A (zh) * 2017-08-28 2017-11-07 中国科学院东北地理与农业生态研究所 水稻HD‑ZIP Ⅳ家族转录因子OsROC4基因、其编码蛋白及其应用
CN110438134A (zh) * 2019-08-28 2019-11-12 江苏省农业科学院 植物叶片卷曲相关蛋白OsRoc8及其编码基因与应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107326033A (zh) * 2017-08-28 2017-11-07 中国科学院东北地理与农业生态研究所 水稻HD‑ZIP Ⅳ家族转录因子OsROC4基因、其编码蛋白及其应用
CN110438134A (zh) * 2019-08-28 2019-11-12 江苏省农业科学院 植物叶片卷曲相关蛋白OsRoc8及其编码基因与应用

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GL2-type homeobox gene Roc4 in rice promotes flowering time preferentially under long ways by repressing Ghd7;Jinhuan Wei et al;《Plant Sci》;20160721;第252卷;133-143 *
Identification of root-preferential transcription factors in rice by analyzing GUS expression patterns of T-DNA tagging lines;Jinhuan Wei et al;《J Plant Biol》;20171231;第60卷;268-277 *
Position dependent expression of GL2-type homeobox gene, Roc1: significance for protoderm differentiation and radial pattern formation in early rice embryogenesis;Momoyo Ito et al;《The Plant Journal》;20020228;第29卷(第4期);497-507 *
Roles of rice GL2-type homeobox genes in epidermis differentiation;Momoyo Ito et al;《Breeding Science》;20031231;第53卷;245-253 *
中国水稻遗传学研究进展与分子设计育种;郭韬 等;《中国科学》;20191012;第49卷(第10期);1185-1212 *

Also Published As

Publication number Publication date
CN112795592A (zh) 2021-05-14

Similar Documents

Publication Publication Date Title
US20220396805A1 (en) Dna sequence for regulating maize leaf angle, and mutant, molecular markers, detection primers, and use thereof
EP1712625A1 (en) An arabidopis thaliana transcription factoras well as the encoding gene and the use thereof
CN110079534B (zh) 调控玉米开花期的基因、启动子及其应用
US20210269817A1 (en) Inhibition of bolting and flowering of a beta vulgaris plant
CN112011567B (zh) 水稻pal1基因及其编码蛋白与应用
CN112390865A (zh) Zm5008基因在调控玉米株高和节间距中的应用
CN113430221B (zh) 番茄wrky37蛋白在调控番茄抗叶片衰老能力、提高番茄产量中的应用
CN109971763A (zh) 花期调控基因cmp1和相关的载体及其应用
CN102212122A (zh) 控制水稻叶绿体发育的突变致死基因及其应用
CN112795592B (zh) OsROC1在水稻育种中的应用
CN113957082B (zh) 一种低温下保护水稻叶绿体发育基因tsa及其编码蛋白质和应用
CN108456683B (zh) 一个调控水稻抽穗期基因sid1的功能及应用
CN114230648B (zh) 水稻基因panda提高植物产量的应用
CN111218457B (zh) 一种水稻mit2基因及其编码蛋白与应用
CN112457385B (zh) 一种控制水稻生育期基因ljp1的应用
CN109182350B (zh) 玉米Zm675基因在植物品质改良中的应用
CN114540375B (zh) 调控玉米开花期和光周期适应性的基因、分子标记及其应用
CN115820667A (zh) 水稻最外层细胞特异性基因6在水稻育种中的作用
CN114672492B (zh) 一种调控水稻株型的基因及其应用
CN111647578B (zh) Usb1蛋白在调控植物抗旱性中的应用
CN111621485B (zh) Usb1蛋白在调控植物产量相关性状中的应用
CN112301034B (zh) 水稻低光响应基因rll1及其突变体与应用
CN108265066B (zh) 与新铁炮百合光周期成花途径相关的基因及其应用
CN117126859A (zh) 一种调控拟南芥器官大小的基因及其应用
CN117305344A (zh) 基因BnaSPL7在独脚金内酯生物合成以及培育转基因植物中的应用

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant