CN114230648B - 水稻基因panda提高植物产量的应用 - Google Patents
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Abstract
本发明涉及水稻基因PANDA提高植物产量的应用。更具体地,本发明涉及的产量性状调控新基因PANDA来源于水稻,该基因表达量的升高导致水稻分蘖数的与单株产量的增加,或者该基因的亚洲稻等位变异在含非洲稻等位变异的表达导致水稻分蘖数和单株产量的增加。研究表明,PANDA过表达转基因株系的分蘖数与产量显著高于野生型,PANDA抑制表达转基因株系的分蘖数与产量显著低于野生型,将PANDA T 通过转基因方法或分子标记辅助选择导入到含PANDA C 等位变异的水稻中,可以增加水稻分蘖数与产量。本发明首次证明PANDA基因具有增强水稻分蘖与产量的功能。本发明为植物高产育种特别是水稻高产育种提供了新的基因资源。
Description
技术领域
本发明属于植物基因工程领域,本发明涉及新基因的功能和应用,具体地涉及水稻分蘖与产量性状调控基因PANDA在增加植物分蘖或分枝与产量方面的功能和应用。
背景技术
据预测,2050年的全球人口总数将接近100亿,那么届时全世界的农业水平要比2005年提高60%-120%才能养活这100亿人口。水稻是重要粮食作物之一,全球有一半以上的人口以稻米作为主食,稻谷产量关系到世界粮食安全。水稻分为亚洲栽培稻(Oryza SativaL.)和非洲栽培稻(Oryza Glaberrrima L.),前者主要种植在我国乃至亚洲地区,后者主要种植在非洲地区。通过自然变异与人为选择,亚洲栽培稻的产量较高。因此,可以从亚洲稻中发掘高产基因,为水稻高产育种尤其是分子育种滞后的非洲栽培稻的高产育种提供优异基因资源。目前,克隆的调控分蘖数与产量的基因有限,这影响了水稻高产分子育种进程。因此,迫切需要挖掘更多新的调控水稻分蘖与产量的新基因,为明晰水稻分蘖数与产量的分子机理和调控网络奠定基础,也为更好更快地培育高产水稻新品种提供基因资源和技术支持,对保障我国粮食安全有着十分重要的意义。
发明内容
本发明属于植物基因工程领域,本发明涉及新基因的功能和应用,具体地涉及水稻株型调控新基因PANDA在增加植物分蘖与产量方面的功能和应用。
本发明人通过遗传筛选到一个控制分蘖数与产量的突变体,通过在该突变体与野生型品种之间杂交构建的遗传群体中,图位克隆到PANDA基因。该基因控制分蘖数与产量性状。通过单倍型分析,确定亚洲稻栽培稻主要等位变异为PANDA T ,非洲稻等位变异为PANDA C ,两者在编码区有1个碱基变异(T/C),导致氨基酸改变(Metionie/Threnine)。通过两种基因型的近等基因系(NIL)比较,发现NIL-PANDA T 的分蘖数与单株产量都显著高于NIL-PANDA C ;在含PANDA C 的品种背景下,PANDA T 的遗传互作的转基因材料的分蘖数、单株颖花数以及单株产量显著高于对照品种。与野生型比较,PANDA T 的遗传互补转基因株系的分蘖数显著性增加。总之,本发明首次证明PANDA(数据库编号:LOC_Os07g07880)基因具有增加植物分蘖与产量的功能,本发明同时为植物高产育种特别是水稻高产育种提供了新的基因资源。
因此,本发明提供下述:
第一方面,水稻新基因PANDA在调控植物分蘖数与产量的功能与应用,但不仅限于此。
第二方面,根据第一方面所述功能与应用,其中所述基因PANDA的核苷酸序列包含从下组核苷酸序列(1)-(7)中选择的核苷酸序列:
(1)序列表中SEQ-ID NO.1.1 、SEQ-ID NO.1.2、SEQ-ID NO.1.3、SEQ-ID NO.2.1 、SEQ-ID NO.2.2、或 SEQ-ID NO.2.3所示的核苷酸序列;
(2)在严格条件下与(1)所限定的核苷酸杂交且编码植物分蘖数与产量相关蛋白的核苷酸序列;
(3)与(1)的核苷酸序列具有至少70%、优选至少80%、更优选至少90%、尤其是至少95%或98%或99%同一性的核苷酸序列;
(4) 与(1)中的核苷酸序列编码相同氨基酸序列的蛋白质、但因遗传密码的简并性而在序列上不同的核苷酸序列;
(5)编码如下氨基酸序列之一的核苷酸序列:SEQ-ID NO.3.1 、SEQ-ID NO.3.2或SEQ-ID NO.3.3所示的氨基酸序列,或者,由于一个或多个(例如1-25个、1-20个,1-15个,1-10个,1-5个,1-3个)氨基酸残基的替代、缺失和/或插入而与SEQ-ID NO.3.1 、SEQ-IDNO.3.2 或SEQ-ID NO.3.3所示的氨基酸序列不同的氨基酸序列,或者,与SEQ-ID NO.3.1、SEQ-ID NO.3.2或SEQ-ID NO.3.3所示的氨基酸序列具有至少70%、优选至少80%、更优选至少90%、尤其是至少95%或98%同一性的氨基酸序列;
(6)(1)-(5)任何一个的核苷酸序列的活性片段;
(7)或与(1)-(5)任何一个的核苷酸序列互补的核苷酸序列。
(8)其中(2)-(7)中 定义的核苷酸序列具有增加植物分蘖或分枝与产量的功能。
本发明还涉及扩增第二方面所述核苷酸序列的全长或者任意片段的引物对,本发明还涉及包含第二方面所述基因的植物表达载体或者克隆载体或者宿主细胞,所述宿主细胞可以选自植物细胞或者微生物细胞,例如大肠杆菌细胞或农杆菌细胞。
第三方面,根据第一方面所述应用,其中所述基因PANDA编码蛋白质的序列,其包含从如下组氨基酸序列(1)-(5)中选择的氨基酸序列:
(1)如SEQ-ID NO.3.1 、SEQ-ID NO.3.2或SEQ-ID NO.3.3所示的氨基酸序列;
(2)由于一个或多个(例如1-25个、1-20个,1-15个,1-10个,1-5个,1-3个)氨基酸残基的替代、缺失和/或插入而与SEQ-ID NO.3所示的氨基酸序列不同的氨基酸序列;
(3)与SEQ-ID NO.3.1、SEQ-ID NO.3.2或SEQ-ID NO.3.3所示的氨基酸序列具有至少70%、优选至少80%、更优选至少90%、尤其是至少95%或98%同一性的氨基酸序列;
(4)(1)或(2)或(3)所述的氨基酸序列的活性片段;
(5)由第二方面所述(1)-(7)组核苷酸分子编码的氨基酸序列。
(6)其中(2)-(5)中 定义的氨基酸序列具有增加植物分蘖数与产量的功能。
第四方面,根据第一方面所述应用,其中所述基因PANDA的启动子序列,其包含从如下组核苷酸序列中选择的核苷酸序列:
(1)序列表中SEQ-ID NO.4所示的核苷酸序列;
(2)与(1)的核苷酸序列具有至少70%、优选至少80%、更优选至少90%、尤其是至少95%或98%或99%同一性的核苷酸序列;
(3)(1)-(2)任何一个的核苷酸序列的活性片段;
(4)或与(1)-(3)任何一个的核苷酸序列互补的核苷酸序列。
本发明还涉及包含第五方面所述启动子序列的构建体。
本发明的有益效果表现在:
(1)本发明首次克隆了水稻PANDA基因,并首次证明了该基因增加植物分蘖数与产量的功能,PANDA基因对进一步阐明植物株型调控机理和遗传网络有着重要的意义。
(2)本发明通过实验验证, PANDA超表达转基因水稻株系的分蘖数显著高于野生型,PANDA T 转基因水稻株系(在自身启动子驱动下)的分蘖数与产量显著高于野生型,近等基因系NIL-PANDA T 的分蘖数与产量显著高于近等基因系NIL-PANDA C 。因此,PANDA基因可用于植物遗传改良,以获得分蘖数与产量都增加的植物。本发明为植物高产育种特别是水稻高产育种提供了新的基因资源,对培养高产作物新品种、保障作物生产安全具有重要的应用价值。
附图说明
图1 panda突变体表型分析,其中,A为水稻粳稻品种台北TP309与突变体材料panda的分蘖数对比,B为水稻粳稻品种台北309与突变体材料panda的籽粒对比,C为水稻粳稻品种台北309与突变体材料panda的有效穗数和千粒重对比,D为水稻粳稻品种台北309与突变体材料panda的粒长、粒宽、粒厚对比。
图2 PADNA基因精细定位。
图3功能互补实验,其中a为水稻粳稻品种台北TP309、突变体材料panda、转基因植株PANDA/panda的种植效果图,b为水稻粳稻品种台北TP309、突变体材料panda、转基因植株PANDA/panda #1、转基因植株PANDA/panda #2在穗数、粒数、结实率、千粒重、粮食产量/株上的对比。
图4 PANDA亚细胞定位,其中A为亚细胞定位载体 35S::PANDA-GFP 转化水稻原生质体进行的显微镜观察,B为含有35S::PANDA-GFP的农杆菌浸染烟草表皮细胞进行的显微镜观察。
图5 PANDA基因表达分析。
具体实施方式
以下实施例对本发明进行进一步描述,这些描述并不是对发明内容作进一步的限定。若未特别指明,实施例中所用的技术手段为本领域技术人员所熟知的常规手段,所用材料、试剂等,如无特殊说明,均可从商业途径获得。
实施例1: 水稻基因PANDA的获得
1、水稻panda突变体的表型及遗传分析
本发明的实施例从水稻粳稻品种台北TP309中获得一个自然突变的突变体材料panda。与亲本材料台北TP309相比(WT),panda具有以下突变表型:在成株期,panda突变体株高增加,分蘖数减少(图1 A),统计分析发现,panda突变体穗数减少44.1%,但是粒重增加42.7%,粒重的增加是由于突变体粒长、粒宽、粒厚变化所致,突变体粒长增加15.9%,粒宽增加17.7%,粒厚增加9%(图1 B, C, D)。
以panda做母本,野生型亲本材料台北TP309 做父本进行杂交,F1的表型与野生型亲本材料台北TP309一致,说明panda是一个隐形突变体材料,F1植株自交得到F2,在F2群体中,野生型植株和panda表型植株的比值符合 3:1,这表明 panda是由隐性单基因控制。
2、PANDA基因的精细定位
将panda突变体与常规籼稻品种特青(Teqing)杂交,获得F1 ,F1自交得到F2。在F2群体中,选出突变体表型单株对PANDA进行定位分析,将PANDA基因精细定位于水稻第7号染色体上约45Kb区间内,在该区间有8个预测基因(图2);提取panda突变体与野生型亲本台北TP309的基因组DNA,对该区间的所有基因进行测序比较分析,发现只有一个预测基因(LOC_Os07g07880 )在编码区有1个SNP突变,导致编码氨基酸发生改变,初步确定LOC_Os07g07880 为PANDA基因的候选基因。该基因的野生型cDNA序列如SEQ-ID NO.1.1 所示,其基因组核苷酸序列如SEQ-ID NO.2.1所示,其编码蛋白质的氨基酸序列如SEQ-ID NO.3.1所示,其启动子核苷酸序列如SEQ-ID NO.4.1 所示。
3、功能互补实验
为了证实候选基因LOC_Os07g07880 确实为控制水稻分蘖和粒重的PANDA基因,本发明从野生型亲本台北TP309中扩增出该基因,构建了互补载体,通过转化农杆菌并侵染突变体 panda诱导的愈伤,最终分化得到转基因植株(PANDA/panda)。对阳性转基因株系PANDA/panda #1、PANDA/panda #2的表型进行统计分析,发现转基因植株的穗粒数、结实率、千粒重、产量等性状恢复了野生型材料表型(图3)。这些结果证实了LOC_Os07g07880 就是PANDA基因。
实施例2: PANDA亚细胞定位
将构建好的亚细胞定位载体 35S::PANDA-GFP 转化水稻原生质体,激光共聚焦显微镜观察GFP信号,结果发现 35S::PANDA-GFP 融合蛋白的GFP信号分布在细胞核部位(图4A),表明 PANDA在细胞核中表达。同时,用含有35S::PANDA-GFP的农杆菌浸染烟草表皮细胞,激光共聚焦显微镜下观察,结果也表明该基因是在细胞核中表达(图4 B)。
实施例3: PANDA 基因表达分析
提取野生型 TP309 植株不同组织及愈伤的RNA,并进行 RT-qPCR 分析,结果表明PANDA基因在水稻愈伤和茎、根、叶、叶鞘、穗中都有转录水平的表达,在水稻不同 组织器官中,PANDA基因在穗子中有较高的表达(图5)。
在培育作物新品种的过程中,可以通过转基因的方法,利用包含本发明提供的基因PANDA的植物表达载体转化受体作物而获得转基因作物植株,进而可以提高受体作物的分蘖数与产量。也可以将含有本发明提供的基因PANDA的作物植株与另一作物植株杂交获得后代作物植株,所获得的后代作物植株其产量得到改良。
虽然,上文中已经用一般性说明及具体实施方案对本发明作了详尽的描述,但在本发明基础上,可以对之作一些修改或改进,这对本领域技术人员而言是显而易见的。因此,在不偏离本发明精神的基础上所做的这些修改或改进,均属于本发明要求保护的范围。
序列表
<110> 三峡大学 中国科学院亚热带农业生态研究所
<120> 水稻基因PANDA提高植物产量的应用
<130>
<160> 10
<210> 1
<211> 1326
<212> DNA
<213> SEQ-ID NO:1.1 (PANDAT的CDS序列,源自亚洲水稻Oryza sativa的cDNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAG GGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGATGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGGCAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAA
<210> 2
<211> 1326
<212> DNA
<213> SEQ-ID NO:1.2 (PANDAC的CDS序列,源自非洲水稻Oryza Glaberrrima的cDNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAGGGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGACGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGGCAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAA
<210> 3
<211> 1326
<212> DNA
<213> SEQ-ID NO:1.3(panda的CDS序列,源自EMS突变体panda的cDNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAGGGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGATGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGACAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAA
<210> 4
<211> 1424
<212> DNA
<213> SEQ-ID NO:2.1 (PANDAT的DNA序列,源自亚洲水稻Oryza sativa的基因组DNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAGGGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTATCGCTTGTTCCTTCCCAAGATTTGGTGCGGTCGACGATTCTTTAGGTTGATTGATTGTTTCGGCGTGATATTCCAATCTTGCAATTCCAATCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGATGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGGCAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAAGAAGCTTCCCATTGAACTTAGCTAAGCTGACTGGCAGTACTCTGGAGTTGCAAGAAGGCATCTCTGTTCTTATGTTTTTCTCCTCAGTTGTCCTTGTTGTAATCAGACCTGCTGGTCTCCATTCGGTAAAGATTAGCAATGAAATAATTCAGTTAGGAATTAGCTAGCTCAGGAGCAAACTATCTCTTCCTTGAGTTAAGGAAAAAATGTTAATGTGTTCATGGTGATGACAATCTCCATCATTTTGAGGTACAAGATATATCAGTGGTCAATTGCTTTGAATGAAGGAAATCGCCTTTAAGGAGAGTAGCTATTCAACTTTGTTTTATAAATGTTTAGATTTGCATAATATAGTAAAACTCATGCTCGCATGTTATTAAAGCATATCCAAGAAAAATAGTAACCTATATATGACATGTTGAGTTGAGTGAACTAGTCTTGGATGTACATCATCTCATTTTCATTTTATTGCAAGGCTATTGTTTTCTAA
<210> 5
<211> 1424
<212> DNA
<213> SEQ-ID NO:2.2 (PANDAC的DNA序列,源自非洲水稻Oryza Glaberrrima的基因组DNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAGGGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTATCGCTTGTTCCTTCCCAAGATTTGGTGCGGTCGACGATTCTTTAGGTTGATTGATTGTTTCGGCGTGATATTCCAATCTTGCAATTCCAATCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGACGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGGCAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAAGAAGCTTCCCATTGAACTTAGCTAAGCTGACTGGCAGTACTCTGGAGTTGCAAGAAGGCATCTCTGTTCTTATGTTTTTCTCCTCAGTTGTCCTTGTTGTAATCAGACCTGCTGGTCTCCATTCGGTAAAGATTAGCAATGAAATAATTCAGTTAGGAATTAGCTAGCTCAGGAGCAAACTATCTCTTCCTTGAGTTAAGGAAAAAATGTTAATGTGTTCATGGTGATGACAATCTCCATCATTTTGAGGTACAAGATATATCAGTGGTCAATTGCTTTGAATGAAGGAAATCGCCTTTAAGGAGAGTAGCTATTCAACTTTGTTTTATAAATGTTTAGATTTGCATAATATAGTAAAACTCATGCTCGCATGTTATTAAAGCATATCCAAGAAAAATAGTAACCTATATATGACATGTTGAGTTGAGTGAACTAGTCTTGGATGTACATCATCTCATTTTCATTTTATTGCAAGGCTATTGTTTTCTAA
<210> 6
<211> 44
<212> DNA
<213> SEQ-ID NO:2.3 (突变体等位panda的DNA序列,源自突变体panda的基因组DNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAGGGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTATCGCTTGTTCCTTCCCAAGATTTGGTGCGGTCGACGATTCTTTAGGTTGATTGATTGTTTCGGCGTGATATTCCAATCTTGCAATTCCAATCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGATGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGACAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAAGAAGCTTCCCATTGAACTTAGCTAAGCTGACTGGCAGTACTCTGGAGTTGCAAGAAGGCATCTCTGTTCTTATGTTTTTCTCCTCAGTTGTCCTTGTTGTAATCAGACCTGCTGGTCTCCATTCGGTAAAGATTAGCAATGAAATAATTCAGTTAGGAATTAGCTAGCTCAGGAGCAAACTATCTCTTCCTTGAGTTAAGGAAAAAATGTTAATGTGTTCATGGTGATGACAATCTCCATCATTTTGAGGTACAAGATATATCAGTGGTCAATTGCTTTGAATGAAGGAAATCGCCTTTAAGGAGAGTAGCTATTCAACTTTGTTTTATAAATGTTTAGATTTGCATAATATAGTAAAACTCATGCTCGCATGTTATTAAAGCATATCCAAGAAAAATAGTAACCTATATATGACATGTTGAGTTGAGTGAACTAGTCTTGGATGTACATCATCTCATTTTCATTTTATTGCAAGGCTATTGTTTTCTAA
<210> 7
<211> 44
<212> 蛋白序列
<213> SEQ-ID NO:3.1 (PANDAT的蛋白序列, Oryza sativa,441aa)
MEKKTKKKNPSKRGRKRGGRGEGREKKVEEISSSSSSRGRGRRRMAPVKKSKKGKRKSKDSGKLKIVKYGGGAPPLPPELRGLDTEWWYTFLHKHSELGLSAPSDEGEAFRYFFRTSRRTFDYICSIVREDLISRPPSGLINIEGRLLSVEKQVAIAMRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIESMEERARHHLVWPGQERMEQIKARFEAESGLPNCCGAIDATHIIMTLPAVESSEDWCDPAKNYSMFLQGIVDDEMRFIDIVTGWPGSMMFSRLLKCSGFFKHCDAGTRLDGPVMVSAENGEIREYIVGNNCYPLLPWLMTPYEGESLSAPMASFNARQKAARTLGPRALSRLKGSWRILNKVMWRPDKNKLPSIILVCCLLHNIIIDCEDELLPDVQLPDHHDTGYSEEKCEQVDPNGKIMRDVITGYLQI
<210> 8
<211> 44
<212> 蛋白序列
<213> SEQ-ID NO:3.2 (PANDAC的蛋白序列, Oryza Glaberrrima,441aa)
MEKKTKKKNPSKRGRKRGGRGEGREKKVEEISSSSSSRGRGRRRMAPVKKSKKGKRKSKDSGKLKIVKYGGGAPPLPPELRGLDTEWWYTFLHKHSELGLSAPSDEGEAFRYFFRTSRRTFDYICSIVREDLISRPPSGLINIEGRLLSVEKQVAIAMRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIESMEERARHHLVWPGQERMEQIKARFEAESGLPNCCGAIDATHIIMTLPAVESSEDWCDPAKNYSMFLQGIVDDEMRFIDIVTGWPGSMTFSRLLKCSGFFKHCDAGTRLDGPVMVSAENGEIREYIVGNNCYPLLPWLMTPYEGESLSAPMASFNARQKAARTLGPRALSRLKGSWRILNKVMWRPDKNKLPSIILVCCLLHNIIIDCEDELLPDVQLPDHHDTGYSEEKCEQVDPNGKIMRDVITGYLQI
<210> 9
<211> 44
<212> 蛋白序列
<213> SEQ-ID NO:3.3 (panda的蛋白序列, Oryza sativa,441aa)
MEKKTKKKNPSKRGRKRGGRGEGREKKVEEISSSSSSRGRGRRRMAPVKKSKKGKRKSKDSGKLKIVKYGGGAPPLPPELRGLDTEWWYTFLHKHSELGLSAPSDEGEAFRYFFRTSRRTFDYICSIVREDLISRPPSGLINIEGRLLSVEKQVAIAMRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIESMEERARHHLVWPGQERMEQIKARFEAESGLPNCCGAIDATHIIMTLPAVESSEDWCDPAKNYSMFLQGIVDDEMRFIDIVTGWPGSMMFSRLLKCSGFFKHCDAGTRLDGPVMVSAENGEIREYIVGNNCYPLLPWLMTPYEGESLSAPMASFNARQKAARTLGPRALSRLKGSWRILNKVMWRPDKNKLPSIILVCCLLHNIIIDCEDELLPDVQLPDHHDTGYSEEKCEQVDPNDKIMRDVITGYLQI
<210> 10
<211> 2609
<212> DNA
SEQ-ID NO:4 (PANDA的启动子序列, Oryza sativa)
GTCGCCCTTGTTCTCCTCGAGGCACTTGCGCAGCGCCGCCACGTCGCACGCCGCCGCCTTCTTCTTCGTCGCCGTCCCGTCCCTTCCGCCGCCTCCGCCTGTTCCCGCCTCCGCCTCCGCCGCCGCCGCTGCTTCCATGGGACCCTGGTGGTGTCCTCTGCAGAGATGGATGAGCGGAAGGCTCGAGGTCGCCGGGAGGAGGCACGCCGCCGCCGCCGCCGCCGCCGGGACGCGAGACGCGAGGGGGAGAGATTGCGCTACAGTTTTCTTTTTTTTCCTCTTTTCTTTTTCTTTTCCTTTTTATTTTTTTAACGTGTGATTATGGTAAGCTAGAGGATTTTTTTAGAAGTAGTAACACTAACATGAATGGAATCCATTCAATTTCAATGGATACAGCCCAGAGACCATAGGTGGAGACTGAAATTTACAAATTTGCAAACTATTTGCTACTCTTATAAGTGGTAAATAAGAATTTGTCACTGGACACACATGTTATAGACGGTAAGTCGCAAATTCTTAATTGCCGCGTCTTAAAAGTGGCAAAAGGTTAAATGCCCCTGTTTTGATACCAATTGAATTTTCTCAAATACTACATTCGTCCAATATAAATTGTAATTCTAATTTGTTTAGCATATATTAAGGTTTGAGTAGAAAGACTATAATGTCTCTTATTAAATGGTGTATAGGTAAGAGTGAAATGGTAGTTGAGGATAAAATAGGAAGAAATTTAAATGAAAAGTGATTAATGAGATCTTTAGTACTCTATTGTAACAATTATTTTGGGACAAATTCAAATCCTAAAAATACAATTATTTTGGGATAGAGGTAGTAAAAAAAAAACAGAGAAACCTATACTAAAACTTAAAGAACTTCTTTGGCATGAAGCAATATTATGAAAATTTTAGAGGAACTGAGCCATTTCGTGTGAAAATTAGTCGAAGTTCATGCGTCTCAAAAGGAGCCCTTGTCATTTCGTTGCATTTAGTTTCCAGTATTAATTTTGTTGTAAGAGCATGTTAATTTGATTGTTCTTTCCTAATTCCTATTAGAATTAATTAGTGCTAATGTATGAAACAATCTTTTAACAACAATAATATGAATTAGCCAATTGTAAAAGTTTCTATTCTATATATATTGTTAGATCATTTTTATCTATCGATAGATTGCTTCCTAGTCTCTACTGCGTATTGTTTCTTAGTATGGGCCGCAAAAGAAAGTGTGTTAGTAATATCTTTTAGTCGTAAAATTTGGGGTATACAAGTCACACAGTTTGAGTTACATGTAAAAGTTTACTAATTTCATTCAAGAATGAGTTTATTAATTGTTTTGGAGTGGTCTTAATTTTATCATTCTGTAAAGAAATGGAATATGAAACTTCATACACCACATGCAAAATTAAATTTATAGTCAGGGTACACAGATAGGCCGGGCTTATTGTTTTGGGCCGGCCTACTTTAAACATATATCTTTAAAGATAGATCGGGAACGTTAATTTCATAACAGGTAGTTAATCAACGGTGTCGATAGTGGGGGCAAGGATAATTAGGGTTTATGATAGGTCATCCTATATATACATGCTATGGACGGCGCCACACACGAGTAAACAAAACCAAGGTAAACGATAAGGTCTATGATAGATCTCACCATACAAAATCGATAGAAGAGCTAGTCGTGTGCAAAGGTGAAGAAACTAAGAACAGGTAAGATCTTTTTCTTTTTTATCAAGATGAAAGTTAGATATTATTTGTTTTGTTGAAAAGTATTAGAAGATTTAGGATTTTTGATTCAGCGAATACATAAAACTAAGAACATGTAAGGTGTCTTTCTTTCTTACTGAGATGGAAGTAATATATATTTGTTTTGTTGAAATGAACTAGAAAAAAATAGATTTAGGATTCTGATTCAGCGCTTGAAAAACTAAACTAAAATCAACAAAAGGACTTCTTTTCACTTCTTTTCTTTTATCTTGTTTTGTTGAAATCTAATAGATAATTTAAGTTTAGGATTCGGGTCTGGCGGGTATGAAAAACTAAAAACATATAGGATCTCTCTTTTCTATTGAGATGAAAATAAAATTTCATTTGTTATATTGAAATGTAGGAGAAATTTAGGGTTCCAACATAGCAAACACAAAAAACTAACTCTTTCATTCTTTTTAACCAGATGGAAGTAAACATTCATTTGGTTTTGTTGATATGTACTTACTTACTCCATCATAAAACATTCTAGCAATTTCTAGCTATGCATTTGAACTTTATCCTTAATTTGTTTTATGAAATTTTTGAGAAAATTTAGATTTTGGATTCCGATCTAGCAAATGCGAAAACCTAAGAAATCTCTTTCATTTTTTATCGAGATGGAAAGAAAATTTTATTTTTGTTTTGTTGAAATGCAACACTATTACTTCAATCCACAAATATAGGAGTAATAATTTTCAGGTATGTATCTGGATATATTCTTATCTATATACATGACAGTGCCCAGAAAGTTATTAGACTGAATTCCTTTTTTACTGAGATGAAATCAAAGATTCCATTTGCCCCAAAAAAATTACTTAAAAAAATGGATTTACACTAGCAAGTATTAAAAATCCTCCATATATAGGAGAG
序列表
<110> 三峡大学 中国科学院亚热带农业生态研究所
<120> 水稻基因PANDA提高植物产量的应用
<130>
<160> 10
<210> 1
<211> 1326
<212> DNA
<213> SEQ-ID NO:1.1 (PANDAT的CDS序列,源自亚洲水稻Oryza sativa的cDNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAG GGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGATGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGGCAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAA
<210> 2
<211> 1326
<212> DNA
<213> SEQ-ID NO:1.2 (PANDAC的CDS序列,源自非洲水稻Oryza Glaberrrima的cDNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAGGGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGACGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGGCAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAA
<210> 3
<211> 1326
<212> DNA
<213> SEQ-ID NO:1.3(panda的CDS序列,源自EMS突变体panda的cDNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAGGGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGATGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGACAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAA
<210> 4
<211> 1424
<212> DNA
<213> SEQ-ID NO:2.1 (PANDAT的DNA序列,源自亚洲水稻Oryza sativa的基因组DNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAGGGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTATCGCTTGTTCCTTCCCAAGATTTGGTGCGGTCGACGATTCTTTAGGTTGATTGATTGTTTCGGCGTGATATTCCAATCTTGCAATTCCAATCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGATGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGGCAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAAGAAGCTTCCCATTGAACTTAGCTAAGCTGACTGGCAGTACTCTGGAGTTGCAAGAAGGCATCTCTGTTCTTATGTTTTTCTCCTCAGTTGTCCTTGTTGTAATCAGACCTGCTGGTCTCCATTCGGTAAAGATTAGCAATGAAATAATTCAGTTAGGAATTAGCTAGCTCAGGAGCAAACTATCTCTTCCTTGAGTTAAGGAAAAAATGTTAATGTGTTCATGGTGATGACAATCTCCATCATTTTGAGGTACAAGATATATCAGTGGTCAATTGCTTTGAATGAAGGAAATCGCCTTTAAGGAGAGTAGCTATTCAACTTTGTTTTATAAATGTTTAGATTTGCATAATATAGTAAAACTCATGCTCGCATGTTATTAAAGCATATCCAAGAAAAATAGTAACCTATATATGACATGTTGAGTTGAGTGAACTAGTCTTGGATGTACATCATCTCATTTTCATTTTATTGCAAGGCTATTGTTTTCTAA
<210> 5
<211> 1424
<212> DNA
<213> SEQ-ID NO:2.2 (PANDAC的DNA序列,源自非洲水稻Oryza Glaberrrima的基因组DNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAGGGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTATCGCTTGTTCCTTCCCAAGATTTGGTGCGGTCGACGATTCTTTAGGTTGATTGATTGTTTCGGCGTGATATTCCAATCTTGCAATTCCAATCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGACGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGGCAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAAGAAGCTTCCCATTGAACTTAGCTAAGCTGACTGGCAGTACTCTGGAGTTGCAAGAAGGCATCTCTGTTCTTATGTTTTTCTCCTCAGTTGTCCTTGTTGTAATCAGACCTGCTGGTCTCCATTCGGTAAAGATTAGCAATGAAATAATTCAGTTAGGAATTAGCTAGCTCAGGAGCAAACTATCTCTTCCTTGAGTTAAGGAAAAAATGTTAATGTGTTCATGGTGATGACAATCTCCATCATTTTGAGGTACAAGATATATCAGTGGTCAATTGCTTTGAATGAAGGAAATCGCCTTTAAGGAGAGTAGCTATTCAACTTTGTTTTATAAATGTTTAGATTTGCATAATATAGTAAAACTCATGCTCGCATGTTATTAAAGCATATCCAAGAAAAATAGTAACCTATATATGACATGTTGAGTTGAGTGAACTAGTCTTGGATGTACATCATCTCATTTTCATTTTATTGCAAGGCTATTGTTTTCTAA
<210> 6
<211> 44
<212> DNA
<213> SEQ-ID NO:2.3 (突变体等位panda的DNA序列,源自突变体panda的基因组DNA)
ATGGAGAAGAAAACCAAAAAGAAGAACCCTAGCAAGAGGGGGAGAAAAAGAGGAGGAAGAGGGGAGGGAAGGGAGAAGAAAGTGGAGGAGATCAGCAGCAGCAGCAGCAGCCGCGGCCGCGGCCGCCGGAGGATGGCGCCGGTGAAGAAGTCCAAGAAAGGGAAGCGCAAGTCCAAGGACTCCGGCAAGCTCAAGATCGTCAAGTATGGCGGCGGCGCCCCTCCCCTCCCCCCCGAGCTCCGCGGCCTCGACACCGAGTGGTGGTACACCTTCCTCCACAAGCACTCCGAGCTAGGTATCGCTTGTTCCTTCCCAAGATTTGGTGCGGTCGACGATTCTTTAGGTTGATTGATTGTTTCGGCGTGATATTCCAATCTTGCAATTCCAATCTAGGTCTGAGCGCGCCGTCAGATGAGGGGGAGGCGTTCAGGTATTTCTTCAGGACGTCGAGGAGGACGTTCGACTACATCTGCTCGATTGTGAGGGAGGATTTGATCTCTAGGCCGCCGTCAGGGCTGATCAACATCGAGGGGAGGCTGCTCAGTGTGGAGAAGCAGGTGGCGATTGCCATGAGGAGGCTGGCGTCGGGCGATTCGCAGGTGTCGGTGGGGGCGGCTTTTGGTGTCGGGCAGTCCACCGTCTCGCAGGTGACTTGGAGGTTCATCGAGTCGATGGAAGAGCGGGCTCGGCATCATCTGGTGTGGCCCGGGCAGGAGAGGATGGAGCAGATCAAGGCGAGGTTCGAGGCCGAGTCCGGTCTGCCGAATTGTTGCGGCGCCATCGATGCGACCCACATTATCATGACGCTTCCTGCTGTCGAGTCGTCTGAGGATTGGTGCGACCCGGCGAAGAATTACAGCATGTTCCTGCAGGGGATTGTTGATGATGAGATGAGGTTTATTGATATTGTCACTGGTTGGCCTGGCAGCATGATGTTTTCGCGGTTGCTGAAGTGCTCTGGGTTTTTCAAGCACTGCGATGCTGGGACTCGCTTGGATGGCCCTGTCATGGTTTCAGCAGAGAATGGAGAAATCAGGGAGTACATTGTTGGTAACAATTGTTATCCTTTACTCCCATGGCTTATGACTCCCTATGAAGGGGAGAGTCTGTCTGCTCCAATGGCCAGCTTTAATGCTAGGCAGAAGGCTGCAAGAACGCTTGGACCAAGAGCACTGTCACGGCTGAAGGGCTCCTGGAGGATCTTAAACAAAGTCATGTGGAGGCCTGATAAGAACAAGTTGCCGAGCATAATTCTTGTCTGCTGTTTGCTTCACAATATAATCATAGACTGTGAAGACGAACTGCTTCCAGATGTACAACTTCCAGATCACCATGATACTGGTTATAGTGAAGAGAAGTGCGAGCAAGTGGATCCTAATGACAAGATAATGAGAGATGTCATTACAGGATATCTTCAAATCTAAGAAGCTTCCCATTGAACTTAGCTAAGCTGACTGGCAGTACTCTGGAGTTGCAAGAAGGCATCTCTGTTCTTATGTTTTTCTCCTCAGTTGTCCTTGTTGTAATCAGACCTGCTGGTCTCCATTCGGTAAAGATTAGCAATGAAATAATTCAGTTAGGAATTAGCTAGCTCAGGAGCAAACTATCTCTTCCTTGAGTTAAGGAAAAAATGTTAATGTGTTCATGGTGATGACAATCTCCATCATTTTGAGGTACAAGATATATCAGTGGTCAATTGCTTTGAATGAAGGAAATCGCCTTTAAGGAGAGTAGCTATTCAACTTTGTTTTATAAATGTTTAGATTTGCATAATATAGTAAAACTCATGCTCGCATGTTATTAAAGCATATCCAAGAAAAATAGTAACCTATATATGACATGTTGAGTTGAGTGAACTAGTCTTGGATGTACATCATCTCATTTTCATTTTATTGCAAGGCTATTGTTTTCTAA
<210> 7
<211> 44
<212> 蛋白序列
<213> SEQ-ID NO:3.1 (PANDAT的蛋白序列, Oryza sativa,441aa)
MEKKTKKKNPSKRGRKRGGRGEGREKKVEEISSSSSSRGRGRRRMAPVKKSKKGKRKSKDSGKLKIVKYGGGAPPLPPELRGLDTEWWYTFLHKHSELGLSAPSDEGEAFRYFFRTSRRTFDYICSIVREDLISRPPSGLINIEGRLLSVEKQVAIAMRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIESMEERARHHLVWPGQERMEQIKARFEAESGLPNCCGAIDATHIIMTLPAVESSEDWCDPAKNYSMFLQGIVDDEMRFIDIVTGWPGSMMFSRLLKCSGFFKHCDAGTRLDGPVMVSAENGEIREYIVGNNCYPLLPWLMTPYEGESLSAPMASFNARQKAARTLGPRALSRLKGSWRILNKVMWRPDKNKLPSIILVCCLLHNIIIDCEDELLPDVQLPDHHDTGYSEEKCEQVDPNGKIMRDVITGYLQI
<210> 8
<211> 44
<212> 蛋白序列
<213> SEQ-ID NO:3.2 (PANDAC的蛋白序列, Oryza Glaberrrima,441aa)
MEKKTKKKNPSKRGRKRGGRGEGREKKVEEISSSSSSRGRGRRRMAPVKKSKKGKRKSKDSGKLKIVKYGGGAPPLPPELRGLDTEWWYTFLHKHSELGLSAPSDEGEAFRYFFRTSRRTFDYICSIVREDLISRPPSGLINIEGRLLSVEKQVAIAMRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIESMEERARHHLVWPGQERMEQIKARFEAESGLPNCCGAIDATHIIMTLPAVESSEDWCDPAKNYSMFLQGIVDDEMRFIDIVTGWPGSMTFSRLLKCSGFFKHCDAGTRLDGPVMVSAENGEIREYIVGNNCYPLLPWLMTPYEGESLSAPMASFNARQKAARTLGPRALSRLKGSWRILNKVMWRPDKNKLPSIILVCCLLHNIIIDCEDELLPDVQLPDHHDTGYSEEKCEQVDPNGKIMRDVITGYLQI
<210> 9
<211> 44
<212> 蛋白序列
<213> SEQ-ID NO:3.3 (panda的蛋白序列, Oryza sativa,441aa)
MEKKTKKKNPSKRGRKRGGRGEGREKKVEEISSSSSSRGRGRRRMAPVKKSKKGKRKSKDSGKLKIVKYGGGAPPLPPELRGLDTEWWYTFLHKHSELGLSAPSDEGEAFRYFFRTSRRTFDYICSIVREDLISRPPSGLINIEGRLLSVEKQVAIAMRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIESMEERARHHLVWPGQERMEQIKARFEAESGLPNCCGAIDATHIIMTLPAVESSEDWCDPAKNYSMFLQGIVDDEMRFIDIVTGWPGSMMFSRLLKCSGFFKHCDAGTRLDGPVMVSAENGEIREYIVGNNCYPLLPWLMTPYEGESLSAPMASFNARQKAARTLGPRALSRLKGSWRILNKVMWRPDKNKLPSIILVCCLLHNIIIDCEDELLPDVQLPDHHDTGYSEEKCEQVDPNDKIMRDVITGYLQI
<210> 10
<211> 2609
<212> DNA
SEQ-ID NO:4 (PANDA的启动子序列, Oryza sativa)
GTCGCCCTTGTTCTCCTCGAGGCACTTGCGCAGCGCCGCCACGTCGCACGCCGCCGCCTTCTTCTTCGTCGCCGTCCCGTCCCTTCCGCCGCCTCCGCCTGTTCCCGCCTCCGCCTCCGCCGCCGCCGCTGCTTCCATGGGACCCTGGTGGTGTCCTCTGCAGAGATGGATGAGCGGAAGGCTCGAGGTCGCCGGGAGGAGGCACGCCGCCGCCGCCGCCGCCGCCGGGACGCGAGACGCGAGGGGGAGAGATTGCGCTACAGTTTTCTTTTTTTTCCTCTTTTCTTTTTCTTTTCCTTTTTATTTTTTTAACGTGTGATTATGGTAAGCTAGAGGATTTTTTTAGAAGTAGTAACACTAACATGAATGGAATCCATTCAATTTCAATGGATACAGCCCAGAGACCATAGGTGGAGACTGAAATTTACAAATTTGCAAACTATTTGCTACTCTTATAAGTGGTAAATAAGAATTTGTCACTGGACACACATGTTATAGACGGTAAGTCGCAAATTCTTAATTGCCGCGTCTTAAAAGTGGCAAAAGGTTAAATGCCCCTGTTTTGATACCAATTGAATTTTCTCAAATACTACATTCGTCCAATATAAATTGTAATTCTAATTTGTTTAGCATATATTAAGGTTTGAGTAGAAAGACTATAATGTCTCTTATTAAATGGTGTATAGGTAAGAGTGAAATGGTAGTTGAGGATAAAATAGGAAGAAATTTAAATGAAAAGTGATTAATGAGATCTTTAGTACTCTATTGTAACAATTATTTTGGGACAAATTCAAATCCTAAAAATACAATTATTTTGGGATAGAGGTAGTAAAAAAAAAACAGAGAAACCTATACTAAAACTTAAAGAACTTCTTTGGCATGAAGCAATATTATGAAAATTTTAGAGGAACTGAGCCATTTCGTGTGAAAATTAGTCGAAGTTCATGCGTCTCAAAAGGAGCCCTTGTCATTTCGTTGCATTTAGTTTCCAGTATTAATTTTGTTGTAAGAGCATGTTAATTTGATTGTTCTTTCCTAATTCCTATTAGAATTAATTAGTGCTAATGTATGAAACAATCTTTTAACAACAATAATATGAATTAGCCAATTGTAAAAGTTTCTATTCTATATATATTGTTAGATCATTTTTATCTATCGATAGATTGCTTCCTAGTCTCTACTGCGTATTGTTTCTTAGTATGGGCCGCAAAAGAAAGTGTGTTAGTAATATCTTTTAGTCGTAAAATTTGGGGTATACAAGTCACACAGTTTGAGTTACATGTAAAAGTTTACTAATTTCATTCAAGAATGAGTTTATTAATTGTTTTGGAGTGGTCTTAATTTTATCATTCTGTAAAGAAATGGAATATGAAACTTCATACACCACATGCAAAATTAAATTTATAGTCAGGGTACACAGATAGGCCGGGCTTATTGTTTTGGGCCGGCCTACTTTAAACATATATCTTTAAAGATAGATCGGGAACGTTAATTTCATAACAGGTAGTTAATCAACGGTGTCGATAGTGGGGGCAAGGATAATTAGGGTTTATGATAGGTCATCCTATATATACATGCTATGGACGGCGCCACACACGAGTAAACAAAACCAAGGTAAACGATAAGGTCTATGATAGATCTCACCATACAAAATCGATAGAAGAGCTAGTCGTGTGCAAAGGTGAAGAAACTAAGAACAGGTAAGATCTTTTTCTTTTTTATCAAGATGAAAGTTAGATATTATTTGTTTTGTTGAAAAGTATTAGAAGATTTAGGATTTTTGATTCAGCGAATACATAAAACTAAGAACATGTAAGGTGTCTTTCTTTCTTACTGAGATGGAAGTAATATATATTTGTTTTGTTGAAATGAACTAGAAAAAAATAGATTTAGGATTCTGATTCAGCGCTTGAAAAACTAAACTAAAATCAACAAAAGGACTTCTTTTCACTTCTTTTCTTTTATCTTGTTTTGTTGAAATCTAATAGATAATTTAAGTTTAGGATTCGGGTCTGGCGGGTATGAAAAACTAAAAACATATAGGATCTCTCTTTTCTATTGAGATGAAAATAAAATTTCATTTGTTATATTGAAATGTAGGAGAAATTTAGGGTTCCAACATAGCAAACACAAAAAACTAACTCTTTCATTCTTTTTAACCAGATGGAAGTAAACATTCATTTGGTTTTGTTGATATGTACTTACTTACTCCATCATAAAACATTCTAGCAATTTCTAGCTATGCATTTGAACTTTATCCTTAATTTGTTTTATGAAATTTTTGAGAAAATTTAGATTTTGGATTCCGATCTAGCAAATGCGAAAACCTAAGAAATCTCTTTCATTTTTTATCGAGATGGAAAGAAAATTTTATTTTTGTTTTGTTGAAATGCAACACTATTACTTCAATCCACAAATATAGGAGTAATAATTTTCAGGTATGTATCTGGATATATTCTTATCTATATACATGACAGTGCCCAGAAAGTTATTAGACTGAATTCCTTTTTTACTGAGATGAAATCAAAGATTCCATTTGCCCCAAAAAAATTACTTAAAAAAATGGATTTACACTAGCAAGTATTAAAAATCCTCCATATATAGGAGAG
Claims (4)
1. 一种参与控制水稻植物株高、粒重、粒宽、粒厚的PANDA基因,其特征在于:核苷酸序列如SEQ-ID NO.2.3所示,其编码蛋白质的氨基酸序列如SEQ-ID NO.3.3 所示。
2.一种包含权利要求1所述基因的植物表达载体。
3.一种宿主细胞,其特征在于:该宿主细胞包含权利要求1所述的基因;所述宿主细胞选自微生物细胞;其中微生物细胞包括大肠杆菌细胞或农杆菌细胞。
4.权利要求1所述的PANDA基因在控制水稻植物株高、粒重、粒宽、粒厚中的应用。
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CN103993018A (zh) * | 2014-03-13 | 2014-08-20 | 中国科学院遗传与发育生物学研究所 | 控制水稻株高、提高抗倒伏能力、增加有效分蘖数和产量的基因及其应用 |
CN110894220A (zh) * | 2018-09-12 | 2020-03-20 | 中国科学院遗传与发育生物学研究所 | 种子相关蛋白在调控植物种子大小中的应用 |
CN111434679A (zh) * | 2019-01-10 | 2020-07-21 | 中国科学院遗传与发育生物学研究所 | 株型相关蛋白在调控植物株型中的应用 |
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