CN112791017A - Haematococcus pluvialis micelle extract for removing wrinkles, repairing and whitening skin and preparation method thereof - Google Patents

Haematococcus pluvialis micelle extract for removing wrinkles, repairing and whitening skin and preparation method thereof Download PDF

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CN112791017A
CN112791017A CN202110108054.XA CN202110108054A CN112791017A CN 112791017 A CN112791017 A CN 112791017A CN 202110108054 A CN202110108054 A CN 202110108054A CN 112791017 A CN112791017 A CN 112791017A
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extract
astaxanthin
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accounting
haematococcus pluvialis
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蔚晨歌
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Sichuan Mayo Youxiang Biotechnology Co ltd
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Sichuan Mayo Youxiang Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706—Algae
    • A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0291—Micelles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/35—Ketones, e.g. benzophenone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/37—Esters of carboxylic acids
    • A61K8/375—Esters of carboxylic acids the alcohol moiety containing more than one hydroxy group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4993—Derivatives containing from 2 to 10 oxyalkylene groups
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/676—Ascorbic acid, i.e. vitamin C
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Abstract

The invention discloses a haematococcus pluvialis micelle extract for removing wrinkles, repairing and whitening skin and a preparation method thereof. The haematococcus pluvialis micelle extract for removing wrinkles, repairing and whitening is prepared from the following raw materials: tween 80 accounting for 10% of the total volume of the extract, 1% astaxanthin concentrated solution accounting for 5-10% of the total volume of the extract, medium-chain triglyceride accounting for 2% of the total volume of the extract, ascorbic acid accounting for 0.05% of the total volume of the extract, and ethylenediaminetetraacetic acid accounting for 0.1% of the total volume of the extract. The haematococcus pluvialis extract prepared by the method micellizes astaxanthin, so that the astaxanthin can form a good dispersion system in oil and water, the application performance of the astaxanthin in a water-soluble system is greatly improved, and the formed astaxanthin-based micelle system has excellent stability.

Description

Haematococcus pluvialis micelle extract for removing wrinkles, repairing and whitening skin and preparation method thereof
Technical Field
The invention relates to the technical field of haematococcus pluvialis extracts, in particular to a haematococcus pluvialis micelle extract for wrinkle removal, repair and whitening and a preparation method thereof.
Background
Natural antioxidants are widely available in nature, and ascorbic acid (vitamin C), tocopherol (vitamin E), tea polyphenol, carotene and the like are common, wherein the most powerful antioxidant is astaxanthin of carotene class. Astaxanthin obtained commercially on a large scale is mainly derived from haematococcus pluvialis, and is fermented, extracted and purified to obtain commercial products with different purities. The astaxanthin has the function of resisting oxidation, contains active ingredients, can eliminate free radicals in cells, enhances the regeneration capability of the cells, can also maintain the balance of the organism, reduces the accumulation of aged cells and enables people to keep young; astaxanthin also has a function of ultraviolet radiation protection, so astaxanthin is widely used in cosmetics and can repair and whiten skin.
The astaxanthin molecule contains a conjugated olefinic chain in the middle and contains hydroxyl ketone groups on two side rings. Astaxanthin has a higher bioavailable activity than other antioxidants because it binds to the cell membrane from the inside and outside. The ring structures on both sides eliminate free radicals inside and outside the cell membrane, while the lipophilic alkene chain in the middle can eliminate free radicals in the cell membrane. After astaxanthin scavenges oxygen radicals, the carotenoid structure is reduced back to the original state after energy transfer is dispersed by the solvent. According to the report of the literature, the antioxidant activity of the astaxanthin is 10 times that of lutein, zeaxanthin and canthaxanthin and 100 times that of alpha tocopherol.
The molecular formula of astaxanthin is as follows:
Figure BDA0002918255770000011
natural antioxidants can be mainly classified into two major groups according to their solubility in water, water-soluble antioxidants such as ascorbic acid, tea polyphenols; oil-soluble antioxidants, such as vitamin E, carotene, astaxanthin. Water-soluble antioxidants are difficult to exert antioxidant effects in oil-based systems, and oil-soluble antioxidants are also difficult to exert antioxidant effects in oil-based systems.
Disclosure of Invention
In view of the technical defects, the invention aims to provide a haematococcus pluvialis micelle extract for removing wrinkles, repairing and whitening skin and a preparation method thereof.
In order to solve the technical problems, the invention adopts the following technical scheme:
the haematococcus pluvialis micelle extract capable of removing wrinkles, repairing and whitening is characterized by being prepared from the following raw materials: tween 80 accounting for 10% of the total volume of the extract, 1% astaxanthin concentrated solution accounting for 5-10% of the total volume of the extract, medium-chain triglyceride accounting for 2% of the total volume of the extract, ascorbic acid accounting for 0.05% of the total volume of the extract, and ethylenediaminetetraacetic acid accounting for 0.1% of the total volume of the extract.
The invention also relates to a preparation method of the haematococcus pluvialis micelle extract for wrinkle removal, repair and whitening, which is characterized by comprising the following specific steps of:
1) adding tween 80, 1% astaxanthin concentrated solution, medium chain triglyceride, ascorbic acid, and ethylenediamine tetraacetic acid into deionized water at a certain proportion, and stirring thoroughly;
2) and (3) shearing the solution obtained in the step (1) at a high speed of 3000rpm, standing, filtering, and centrifuging to form stable and transparent micellar haematococcus pluvialis extract liquid.
The invention has the beneficial effects that: the astaxanthin is micellized, so that a good dispersion system can be formed in oil and water, the application performance of the astaxanthin in a water-soluble system is greatly improved, and the formed astaxanthin micelle system has excellent stability.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a graph showing the change in particle size measured by dynamic light scattering provided in example 2 of the present invention;
FIG. 2 is a graph showing the variation of astaxanthin content in samples measured at different irradiation times, which is obtained by fluorescence irradiation of a stock solution of nano-astaxanthin, micro-emulsified astaxanthin and 1% astaxanthin according to example 3 of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The haematococcus pluvialis micelle extract for removing wrinkles, repairing and whitening skin is prepared from the following raw materials: tween 80 accounting for 10% of the total volume of the extract, 1% astaxanthin concentrated solution accounting for 5-10% of the total volume of the extract, medium-chain triglyceride accounting for 2% of the total volume of the extract, ascorbic acid accounting for 0.05% of the total volume of the extract, and ethylenediaminetetraacetic acid accounting for 0.1% of the total volume of the extract.
The preparation method of the haematococcus pluvialis micelle extract for removing wrinkles, repairing and whitening is characterized by comprising the following specific steps of:
1) adding tween 80, 1% astaxanthin concentrated solution, medium chain triglyceride, ascorbic acid, and ethylenediamine tetraacetic acid into deionized water at a certain proportion, and stirring thoroughly;
2) and (3) shearing the solution obtained in the step (1) at a high speed of 3000rpm, standing, filtering, and centrifuging to form stable and transparent micellar haematococcus pluvialis extract liquid.
The mixture of the extracts of Haematococcus pulvialis obtained in this example was compared with a commercially available astaxanthin solution (containing 500ppm ascorbic acid) for the stability of fluorescence irradiation. The samples obtained in this way have the best photostability every 12 hours, as the disappearance of the colour is observed visually.
Example 2
Adding 12% Tween 80, 10% astaxanthin oil concentrate 1%, and 2% MCT (medium chain triglyceride) into deionized water, stirring, shearing at 3000rpm, standing, centrifuging to obtain stable transparent liquid.
The light transmittance was measured at 500nm to check the transparency. Experiments found that the transmittance remained constant after more than 10% tween and more than 2% MCT (medium chain triglyceride) in the above system. The particle size was 10-20nm as measured by dynamic light scattering.
Example 3
The haematococcus pluvialis micellar extract sample prepared in the example 1 for removing wrinkles, repairing and whitening skin is diluted into 0.05% diluent by water together with commercially available nano-astaxanthin, micro-emulsified astaxanthin and 1% astaxanthin stock solution, and 500ppm ascorbic acid is added.
The samples were subjected to fluorescent irradiation and the astaxanthin content of the samples was measured for different irradiation times to compare the stability. The results show that the micellization treated astaxanthin retained the highest astaxanthin content.
The astaxanthin is micellized, so that a good dispersion system can be formed in oil and water, the application performance of the astaxanthin in a water-soluble system is greatly improved, and the formed astaxanthin micelle system has excellent stability; the haematococcus pluvialis micelle extract prepared by the invention is applied to cosmetic products, and can achieve better effects of repairing and whitening skin.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims (2)

1. The haematococcus pluvialis micelle extract capable of removing wrinkles, repairing and whitening is characterized by being prepared from the following raw materials: tween 80 accounting for 10% of the total volume of the extract, 1% astaxanthin concentrated solution accounting for 5-10% of the total volume of the extract, medium-chain triglyceride accounting for 2% of the total volume of the extract, ascorbic acid accounting for 0.05% of the total volume of the extract, and ethylenediaminetetraacetic acid accounting for 0.1% of the total volume of the extract.
2. The preparation method of the haematococcus pluvialis micelle extract with the functions of wrinkle removal, repair and whitening is characterized by comprising the following specific steps:
1) adding tween 80, 1% astaxanthin concentrated solution, medium chain triglyceride, ascorbic acid, and ethylenediamine tetraacetic acid into deionized water at a certain proportion, and stirring thoroughly;
2) and (3) shearing the solution obtained in the step (1) at a high speed of 3000rpm, standing, filtering, and centrifuging to form stable and transparent micellar haematococcus pluvialis extract liquid.
CN202110108054.XA 2021-01-27 2021-01-27 Haematococcus pluvialis micelle extract for removing wrinkles, repairing and whitening skin and preparation method thereof Pending CN112791017A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114306196A (en) * 2021-12-31 2022-04-12 深圳市瑞茵生物科技有限公司 Preparation method of self-assembled whitening active compound micelle and skin care product

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080220102A1 (en) * 2005-07-08 2008-09-11 Dariush Behnam Solubilsation Products of an Active Ingredient Extract
US20110293678A1 (en) * 2008-12-01 2011-12-01 Aquanova Ag Micellarly integrated oxidation protection for natural dyes
CN111011858A (en) * 2020-01-08 2020-04-17 中国农业科学院农产品加工研究所 Mixed oil emulsion for improving bioavailability of fat-soluble active substances and preparation method thereof
CN111328953A (en) * 2020-03-02 2020-06-26 大连医诺生物股份有限公司 Astaxanthin nanoemulsion with good reconstitution property and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080220102A1 (en) * 2005-07-08 2008-09-11 Dariush Behnam Solubilsation Products of an Active Ingredient Extract
US20110293678A1 (en) * 2008-12-01 2011-12-01 Aquanova Ag Micellarly integrated oxidation protection for natural dyes
CN111011858A (en) * 2020-01-08 2020-04-17 中国农业科学院农产品加工研究所 Mixed oil emulsion for improving bioavailability of fat-soluble active substances and preparation method thereof
CN111328953A (en) * 2020-03-02 2020-06-26 大连医诺生物股份有限公司 Astaxanthin nanoemulsion with good reconstitution property and preparation method thereof

Non-Patent Citations (1)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114306196A (en) * 2021-12-31 2022-04-12 深圳市瑞茵生物科技有限公司 Preparation method of self-assembled whitening active compound micelle and skin care product
CN114306196B (en) * 2021-12-31 2024-01-23 深圳市瑞茵电子科技有限公司 Preparation method of self-assembled whitening active compound micelle and skin care product

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