CN115400080B - Pickering emulsion stabilized by luteolin and preparation method and application thereof - Google Patents
Pickering emulsion stabilized by luteolin and preparation method and application thereof Download PDFInfo
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- CN115400080B CN115400080B CN202211005668.6A CN202211005668A CN115400080B CN 115400080 B CN115400080 B CN 115400080B CN 202211005668 A CN202211005668 A CN 202211005668A CN 115400080 B CN115400080 B CN 115400080B
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- 239000000839 emulsion Substances 0.000 title claims abstract description 92
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 title claims abstract description 70
- LRDGATPGVJTWLJ-UHFFFAOYSA-N luteolin Natural products OC1=CC(O)=CC(C=2OC3=CC(O)=CC(O)=C3C(=O)C=2)=C1 LRDGATPGVJTWLJ-UHFFFAOYSA-N 0.000 title claims abstract description 70
- IQPNAANSBPBGFQ-UHFFFAOYSA-N luteolin Chemical compound C=1C(O)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C(O)=C1 IQPNAANSBPBGFQ-UHFFFAOYSA-N 0.000 title claims abstract description 70
- 235000009498 luteolin Nutrition 0.000 title claims abstract description 70
- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 41
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 27
- 229940106134 krill oil Drugs 0.000 claims abstract description 17
- 239000013543 active substance Substances 0.000 claims abstract description 16
- 238000002156 mixing Methods 0.000 claims abstract description 12
- 239000008367 deionised water Substances 0.000 claims abstract description 10
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 7
- 238000002390 rotary evaporation Methods 0.000 claims abstract description 3
- MDKCFLQDBWCQCV-UHFFFAOYSA-N benzyl isothiocyanate Chemical group S=C=NCC1=CC=CC=C1 MDKCFLQDBWCQCV-UHFFFAOYSA-N 0.000 claims description 50
- QAADZYUXQLUXFX-UHFFFAOYSA-N N-phenylmethylthioformamide Natural products S=CNCC1=CC=CC=C1 QAADZYUXQLUXFX-UHFFFAOYSA-N 0.000 claims description 25
- 239000000725 suspension Substances 0.000 claims description 16
- 239000003814 drug Substances 0.000 claims description 4
- 239000002537 cosmetic Substances 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 2
- 238000000265 homogenisation Methods 0.000 claims description 2
- 239000003921 oil Substances 0.000 abstract description 39
- 239000002245 particle Substances 0.000 abstract description 21
- 230000014759 maintenance of location Effects 0.000 abstract description 10
- 239000008157 edible vegetable oil Substances 0.000 abstract description 8
- 239000000463 material Substances 0.000 abstract description 4
- 239000003995 emulsifying agent Substances 0.000 abstract description 2
- 235000019198 oils Nutrition 0.000 description 36
- 239000000243 solution Substances 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 7
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
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- 239000000944 linseed oil Substances 0.000 description 4
- 235000021388 linseed oil Nutrition 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000003064 anti-oxidating effect Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 150000008442 polyphenolic compounds Chemical class 0.000 description 3
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- 241000894006 Bacteria Species 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
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- 238000005259 measurement Methods 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- -1 flavonoid compounds Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
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- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
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- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000010525 oxidative degradation reaction Methods 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
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- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- 229940088594 vitamin Drugs 0.000 description 1
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Classifications
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Abstract
The invention relates to the technical field of emulsion preparation, in particular to a Pickering emulsion stabilized by luteolin, and a preparation method and application thereof. The method of the invention comprises the following steps: s1, preparing an oil phase: dissolving a fat-soluble active substance in antarctic krill oil; s2, preparation of a water phase: dissolving luteolin in absolute ethyl alcohol, slowly injecting deionized water into the luteolin, and performing rotary evaporation to completely volatilize the absolute ethyl alcohol; s3, mixing the oil phase with the water phase, and dispersing at a high speed; s4, homogenizing to obtain Pickering emulsion stabilized by luteolin. According to the invention, luteolin is adopted as an emulsifier, specific edible oil is selected as an oil phase, fat-soluble active substances are used as embedding materials, the luteolin can be encapsulated through Pickering emulsion, and finally, the Pickering emulsion stabilized by the luteolin is prepared by considering all indexes. The preparation method is simple and convenient to operate, and the prepared emulsion has the advantages of strong photostability, small average particle size, high viscosity, high retention rate of fat-soluble active substances and good stability.
Description
Technical Field
The invention relates to the technical field of emulsion preparation, in particular to a Pickering emulsion stabilized by luteolin, and a preparation method and application thereof.
Background
Fat-soluble active substances such as phospholipids, fat-soluble vitamins, polyunsaturated fatty acids and the like have various health-beneficial effects, but are difficult to absorb by human bodies due to the characteristics of poor water solubility, easy oxidative degradation, low bioavailability and the like, so that the functional characteristics of the fat-soluble active substances cannot be fully exerted, and the application of the fat-soluble active substances in the fields of foods, medicines and the like is severely limited. To overcome these obstacles, emulsions are often chosen to achieve delivery of fat-soluble actives, and this approach works well in active delivery system applications.
Emulsions stabilized by solid particles in place of surfactants are collectively referred to as Pickering emulsions. Compared with the traditional emulsion, the Pickering emulsion has the properties of lipid oxidation resistance, strong stability, low toxicity, high safety, low cost and the like, is more favorable for loading the fat-soluble active substances, and has been widely applied to an embedding delivery system. Food grade solid particles commonly used in recent years to stabilize Pickering emulsions are proteins, polysaccharides, polyphenols, and the like. With the continuous expansion of the application of emulsions in the food industry, the development and development of food grade Pickering emulsions has become a hotspot for research.
Luteolin is a natural polyphenol component with biological characteristics widely distributed in various plants, and belongs to weak acid tetrahydroxy flavonoid compounds. The structure of the material contains polyhydroxy structure, so the material has strong antioxidation property. Studies show that luteolin also has various pharmacological effects of resisting bacteria, resisting inflammation, resisting cancer, protecting liver, protecting kidney and the like, and is a functional compound with development potential and market value. However, reports on luteolin-stable Pickering emulsions are seen in the prior art. Moreover, because the interaction between the solid particles and the oil phase is complex, a great deal of technical problems need to be overcome in developing a Pickering emulsion stabilized by luteolin with better stability.
Disclosure of Invention
Technical problems: the development of the Pickering emulsion stabilized by luteolin, which has the advantages of simple and convenient operation, small average particle size of the emulsion, high storage stability, strong light stability, large apparent shear viscosity and high retention rate of fat-soluble active substances, and the preparation method thereof.
The technical scheme is as follows:
the first aim of the invention is to provide a preparation method of Pickering emulsion stabilized by luteolin, which comprises the following steps:
s1, preparing an oil phase: dissolving fat-soluble active substances in antarctic krill oil to prepare a fat-soluble active substance-antarctic krill oil solution;
s2, preparation of a water phase: dissolving luteolin in absolute ethyl alcohol, slowly injecting deionized water into the solution, and performing rotary evaporation to completely volatilize the absolute ethyl alcohol to prepare a luteolin suspension;
s3, mixing: mixing the oil phase prepared in the step S1 with the water phase prepared in the step S2, and dispersing at a high speed;
s4, homogenizing: homogenizing the product obtained in the step S3 to obtain the Pickering emulsion stabilized by luteolin.
As an embodiment of the present invention, the fat-soluble active substance includes benzyl isothiocyanate.
As one embodiment of the invention, the mass concentration of benzyl isothiocyanate in the fat-soluble active substance-antarctic krill oil solution in the step S1 is 3-9 mg/mL.
As one embodiment of the invention, the mass concentration of benzyl isothiocyanate in the fat-soluble active substance-antarctic krill oil solution in the step S1 is 5mg/mL.
As one embodiment of the invention, the mass concentration of luteolin in the luteolin suspension in the step S2 is 2-6 mg/mL.
As one embodiment of the invention, the mass concentration of luteolin in the luteolin suspension in the step S2 is 4mg/mL.
As an embodiment of the present invention, the volume of the oil phase in the step S3 is 5% to 15% of the volume of the water phase.
As an embodiment of the present invention, the oil phase volume in the step S3 is 10% of the water phase volume.
As an embodiment of the present invention, the step S3 is high-speed dispersion: 9000-14000 r/min and dispersing for 2-3 min. Preferably, 10000r/min is dispersed at high speed for 2min.
As an embodiment of the present invention, in the step S4, the homogenization is performed: homogenizing at 5000-10000 psi for 3-5 times. Preferably 7000psi high pressure homogenizes 3 times.
As one embodiment of the invention, the preparation method of the Pickering emulsion stabilized by luteolin comprises the following steps:
s1, preparing an oil phase: dissolving benzyl isothiocyanate in antarctic krill oil to prepare benzyl isothiocyanate-edible oil solution (oil phase) with the mass concentration of 3-9 mg/mL;
s2, preparation of a water phase: dissolving luteolin in absolute ethyl alcohol, slowly injecting deionized water with the volume of 4-8 times of the absolute ethyl alcohol into the absolute ethyl alcohol, completely volatilizing the absolute ethyl alcohol by adopting a rotary evaporator, and preparing a luteolin suspension (water phase) with the mass concentration of 2-6 mg/mL;
s3, mixing: mixing the oil phase prepared in the step S1 with the water phase prepared in the step S2 according to the addition amount of 5-15% of the volume of the water phase, and dispersing at a high speed of 9000-14000 r/min for 2-3 min;
s4, homogenizing: homogenizing under high pressure of 5000-10000 psi for 3-5 times to obtain luteolin Pickering emulsion.
The second object of the invention is to provide a Pickering emulsion stabilized by luteolin, which is prepared by the method.
The third object of the invention is to provide an application of the Pickering emulsion stabilized by luteolin in preparing medicines or cosmetics.
The beneficial effects of the invention are as follows:
(1) The invention is based on the stable Pickering emulsion of solid particles of luteolin, has natural components of luteolin, wide sources and high biological safety, has strong antioxidation property, has various pharmacological effects of resisting bacteria, resisting inflammation, resisting cancer, protecting liver and kidney, and the like, can improve the antioxidation stability and the nutrition property of the Pickering emulsion, and can be used in the fields of medicines and cosmetics. According to the invention, luteolin is used as an emulsifier to be applied to a Pickering emulsion system, so that the application range of natural polyphenol active substances can be enlarged, and a research foundation is provided for effective utilization of fat-soluble active substances.
(2) In the invention, luteolin is dispersed in Pickering emulsion in a low-solubility particle form and is tightly adsorbed on an oil-water interface, so that an emulsion system is stable.
(3) According to the research of the invention, for specific fat-soluble active substances, namely benzyl isothiocyanate, in the preparation process of a Pickering emulsion system embedded with benzyl isothiocyanate, the selection of solid particles and oil phases has great influence on the stability of the system and the retention rate of benzyl isothiocyanate: the average particle size of the luteolin Pickering emulsion prepared by taking antarctic krill oil as an oil phase is smaller, the retention rate of benzyl isothiocyanate is higher (up to 65.25+/-1.45 percent, as shown in figure 8), the average particle size of the fresh emulsion is smaller (194.27 +/-2.06 nm, as shown in figure 6), the average particle size of the emulsion is hardly changed after being stored for 14 days at 4 ℃, compared with the average particle size of the fresh emulsion which is only changed by +1.86nm, as shown in figure 6), the apparent shear viscosity is higher (as shown in figure 7), the light stability is stronger (figures 9-10), and the luteolin Pickering emulsion prepared by taking antarctic krill oil as the oil phase is more stable in a comprehensive view.
(4) The method disclosed by the invention is simple and convenient to operate, and the prepared emulsion has the advantages of small average particle size, high storage stability, strong light stability, large apparent shear viscosity and high retention rate of benzyl isothiocyanate.
Drawings
FIG. 1 is a physical diagram showing the product emulsions prepared in examples 1 to 3 of the present invention stored at 4℃for 0 days;
FIG. 2 is a physical diagram showing the product emulsions prepared in examples 1 to 3 of the present invention stored at 4℃for 1 day;
FIG. 3 is a physical diagram showing the product emulsions prepared in examples 1 to 3 of the present invention stored at 4℃for 4 days;
FIG. 4 is a physical diagram showing the product emulsions prepared in examples 1 to 3 of the present invention stored at 4℃for 7 days;
FIG. 5 is a physical diagram showing the product emulsions prepared in examples 1 to 3 of the present invention stored at 4℃for 14 days;
FIG. 6 shows the average particle diameters of the product emulsions prepared in examples 1 to 3 of the present invention stored at 4℃for 0 day, 1 day, 4 days, 7 days and 14 days, respectively;
FIG. 7 is an illustration of the apparent shear viscosity of the product emulsions prepared in examples 1-3 of the present invention;
FIG. 8 shows benzyl isothiocyanate retention rates of the product emulsions prepared in examples 1 to 3 of the present invention, respectively, stored at 4℃for 14 days;
FIG. 9 is a physical diagram of the product emulsions prepared in examples 1 to 3 of the present invention after being left for 2 hours under different illumination conditions;
FIG. 10 shows the change in turbidity of the emulsion of the product emulsions prepared in examples 1 to 3 according to the invention after being left for 2 hours under different light conditions.
Detailed Description
Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
The invention provides a preparation method of Pickering emulsion stabilized by luteolin, which comprises the following preparation steps:
s1, preparing an oil phase: dissolving benzyl isothiocyanate into edible oil to prepare benzyl isothiocyanate-edible oil solution (oil phase) with the mass concentration of 3-9 mg/mL;
s2, preparation of a water phase: dissolving luteolin in absolute ethyl alcohol, slowly injecting deionized water with the volume of 4-8 times of the absolute ethyl alcohol into the absolute ethyl alcohol, completely volatilizing the absolute ethyl alcohol by adopting a rotary evaporator, and preparing a luteolin suspension (water phase) with the mass concentration of 2-6 mg/mL;
s3, mixing the oil phase prepared in the step S1 with the luteolin suspension prepared in the step S2 according to the addition amount of 5% -15% of the volume of the water phase, and dispersing at a high speed of 9000-14000 r/min for 2-3 min;
s4, homogenizing for 2-5 times under high pressure of 5000-10000 psi to obtain the luteolin Pickering emulsion.
Example 1 (corn oil)
A preparation method of Pickering emulsion stabilized by luteolin comprises the following preparation steps:
s1, preparing an oil phase: dissolving benzyl isothiocyanate in corn oil to prepare benzyl isothiocyanate-edible oil solution (oil phase) with the mass concentration of 5 mg/mL;
s2, preparation of a water phase: dissolving luteolin in absolute ethyl alcohol, slowly injecting deionized water with the volume of 6 times of absolute ethyl alcohol, completely volatilizing the absolute ethyl alcohol by adopting a rotary evaporator, and preparing into a luteolin suspension (water phase) with the mass concentration of 4 mg/mL;
s3, mixing the oil phase prepared in the step S1 with the luteolin suspension prepared in the step S2 according to the addition amount of 10% of the volume of the water phase, and dispersing at a high speed of 10000r/min for 2min;
s4, homogenizing at high pressure for 3 times at 7000psi to obtain the luteolin Pickering emulsion.
Example 2 (Linseed oil)
A preparation method of Pickering emulsion stabilized by luteolin comprises the following preparation steps:
s1, preparing an oil phase: dissolving benzyl isothiocyanate into linseed oil to prepare benzyl isothiocyanate-edible oil solution (oil phase) with the mass concentration of 5 mg/mL;
s2, preparation of a water phase: dissolving luteolin in absolute ethyl alcohol, slowly injecting deionized water with the volume of 6 times of absolute ethyl alcohol, completely volatilizing the absolute ethyl alcohol by adopting a rotary evaporator, and preparing into a luteolin suspension (water phase) with the mass concentration of 4 mg/mL;
s3, mixing the oil phase prepared in the step S1 with the luteolin suspension prepared in the step S2 according to the addition amount of 10% of the volume of the water phase, and dispersing at a high speed of 10000r/min for 2min;
s4, homogenizing at high pressure for 3 times at 7000psi to obtain the luteolin Pickering emulsion.
Example 3 (Antarctic krill oil)
A preparation method of Pickering emulsion stabilized by luteolin comprises the following preparation steps:
s1, preparing an oil phase: dissolving benzyl isothiocyanate in antarctic krill oil to prepare benzyl isothiocyanate-edible oil solution (oil phase) with the mass concentration of 5 mg/mL;
s2, preparation of a water phase: dissolving luteolin in absolute ethyl alcohol, slowly injecting deionized water with the volume of 6 times of absolute ethyl alcohol, completely volatilizing the absolute ethyl alcohol by adopting a rotary evaporator, and preparing into a luteolin suspension (water phase) with the mass concentration of 4 mg/mL;
s3, mixing the oil phase prepared in the step S1 with the luteolin suspension prepared in the step S2 according to the addition amount of 10% of the volume of the water phase, and dispersing at a high speed of 10000r/min for 2min;
s4, homogenizing at high pressure for 3 times at 7000psi to obtain the luteolin Pickering emulsion.
Example 4
A preparation method of Pickering emulsion stabilized by luteolin comprises the following preparation steps:
s1, preparing an oil phase: dissolving benzyl isothiocyanate in antarctic krill oil to prepare benzyl isothiocyanate-edible oil solution (oil phase) with the mass concentration of 3-9 mg/mL;
s2, preparation of a water phase: dissolving luteolin in absolute ethyl alcohol, slowly injecting deionized water with the volume of 6 times of the absolute ethyl alcohol into the absolute ethyl alcohol, completely volatilizing the absolute ethyl alcohol by adopting a rotary evaporator, and preparing a luteolin suspension (water phase) with the mass concentration of 2-6 mg/mL;
s3, mixing the oil phase prepared in the step S1 with the luteolin suspension prepared in the step S2 according to the addition amount of 5% -15% of the volume of the water phase, and dispersing at a high speed of 9000-14000 r/min for 2-3 min;
s4, homogenizing for 3-5 times under high pressure of 5000-10000 psi to obtain the luteolin Pickering emulsion.
The testing method comprises the following steps:
appearance change observation and emulsion particle size measurement: taking a proper amount of the product emulsion prepared in examples 1-3 (stored for 0 day, 1 day, 4 days, 7 days and 14 days at 4 ℃) and observing the appearance change; meanwhile, a proper amount of the product emulsion prepared in examples 1 to 3 (stored for 0 day, 1 day, 4 days, 7 days and 14 days at 4 ℃) is diluted by 100 times, and then the average particle size is detected by a nano particle sizer. Different uppercase letters represent different oil phase significance differences at the same shelf time, and different lowercase letters represent the same oil phase significance differences at different shelf times (p < 0.05). Specific analysis and theoretical basis include:
(1) The appearance and the shape of the emulsion are not obviously changed, and the more stable the system is.
(2) The smaller the average particle size of the emulsion, the more stable the system.
Apparent shear viscosity measurement: the emulsion apparent viscosity was determined using a rheometer equipped with a plate, taking the appropriate amount of fresh product emulsion prepared in examples 1-3. And sucking proper amount of emulsion drop on the measuring platform to make the emulsion cover the two plates homogeneously without bubble and with shearing rate of 0.1-100rad/s. The change in apparent viscosity of the product emulsions prepared in examples 1 to 3 was measured. The specific analysis and theoretical basis are: the higher the viscosity of the emulsion, the slower the settling velocity of its oil droplets and the more stable the system.
Determination of benzyl isothiocyanate retention: and (3) taking a proper amount of the product emulsion prepared in examples 1-3, storing for 14 days at 4 ℃, extracting benzyl isothiocyanate in the emulsion by using normal hexane and methanol, analyzing the content of the benzyl isothiocyanate by adopting high performance liquid chromatography, and calculating the retention rate of the benzyl isothiocyanate in the emulsion according to the positive correlation between the concentration of the benzyl isothiocyanate in the solution and the peak content, wherein the retention rate (%) = the peak area of the benzyl isothiocyanate in the emulsion/the peak area of the benzyl isothiocyanate in the standard substance. Different capital letters represent different oil phase significance differences (p < 0.05) for the same shelf life. The specific analysis and theoretical basis are: the higher the embedding rate of the emulsion, the more stable the system.
Light stability analysis: the product emulsions prepared in examples 1 to 3 of the present invention were subjected to photostability analysis by different illumination for a short period of time, and photostability was reflected by emulsion turbidity. The freshly prepared emulsions of examples 1 to 3, which were treated with different illumination conditions (natural light, ultraviolet light), were each diluted 20 times with deionized water and absorbance was measured at a wavelength of 500nm using an ultraviolet-visible spectrophotometer, and turbidity calculations were performed. The turbidity calculation method comprises the following steps: r is R t ＝(A f -A 1 )/(A b -A 2 ) X 100%, wherein: a is that b -absorbance value of freshly prepared dilution medium; a is that f -absorbance of the dilution medium after operation under different light conditions; a is that 2 Absorbance of the dilution medium prior to experimental operation; a is that 1 -absorbance of the dilution medium after operation under different light conditions; 100-conversion factor.
Different capital letters in the data represent different oil phase significance differences for the same lighting conditions, and different capital letters represent the same oil phase significance differences (p < 0.05) for different lighting conditions. Specific analysis and theoretical basis include:
(1) The appearance and the shape of the emulsion are not obviously changed, and the more stable the system is.
(2) The greater the turbidity of the emulsion, the more stable the system.
Analysis of results:
according to the invention, by comparing the research of constructing luteolin Pickering emulsion conveying systems by adopting different oil phases (corn oil, linseed oil and antarctic krill oil) and embedding benzyl isothiocyanate, the research shows that:
compared with the luteolin Pickering emulsion prepared by taking corn oil and linseed oil as oil phases, the luteolin Pickering emulsion prepared by taking antarctic krill oil as the oil phases has smaller average particle size, higher retention rate of benzyl isothiocyanate (as shown in figure 8) and almost no change of the average particle size of the emulsion after being stored for 14 days at 4 ℃ because of smaller average particle size (194.27 +/-2.06 nm as shown in figure 6) and higher apparent shear viscosity (as shown in figure 7) and higher light stability (figures 9-10) as compared with the average particle size of the fresh emulsion which is only changed by +1.86nm as shown in figure 6), and the luteolin Pickering emulsion prepared by taking antarctic krill oil as the oil phases is more stable in the comprehensive view.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should be covered by the protection scope of the present invention by making equivalents and modifications to the technical solution and the inventive concept thereof.
Claims (6)
1. A preparation method of a Pickering emulsion stabilized by luteolin is characterized by comprising the following steps:
s1, preparing an oil phase: dissolving fat-soluble active substances in antarctic krill oil to prepare a fat-soluble active substance-antarctic krill oil solution;
s2, preparation of a water phase: dissolving luteolin in absolute ethyl alcohol, slowly injecting deionized water into the solution, and performing rotary evaporation to completely volatilize the absolute ethyl alcohol to prepare a luteolin suspension;
s3, mixing: mixing the oil phase prepared in the step S1 with the water phase prepared in the step S2, and dispersing at a high speed;
s4, homogenizing: homogenizing the product obtained in the step S3 to obtain Pickering emulsion stabilized by luteolin; the fat-soluble active substance is benzyl isothiocyanate;
wherein, in the step S1, the mass concentration of the fat-soluble active substance in the fat-soluble active substance-antarctic krill oil solution is 3-9 mg/mL; the mass concentration of luteolin in the luteolin suspension in the step S2 is 2-6 mg/mL; in the step S3, the volume of the oil phase is 5-15% of the volume of the water phase.
2. The method according to claim 1, wherein the oil phase volume in step S3 is 10% of the water phase volume.
3. The method according to claim 1, wherein the step S3 is high-speed dispersion: 9000-14000 r/min and dispersing for 2-3 min.
4. The method according to claim 1, wherein in step S4, the homogenization is performed: homogenizing at 5000-10000 psi for 3-5 times.
5. A Pickering emulsion stabilized by luteolin, characterized in that it is prepared by the method according to any one of claims 1-4.
6. Use of a luteolin-stabilised Pickering emulsion according to claim 5 in the preparation of a medicament or cosmetic.
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