CN112790066A - Method for preparing straw rotting type edible fungus culture medium by using pure corncobs - Google Patents

Method for preparing straw rotting type edible fungus culture medium by using pure corncobs Download PDF

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Publication number
CN112790066A
CN112790066A CN202110016055.1A CN202110016055A CN112790066A CN 112790066 A CN112790066 A CN 112790066A CN 202110016055 A CN202110016055 A CN 202110016055A CN 112790066 A CN112790066 A CN 112790066A
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CN
China
Prior art keywords
corncobs
corncob
corn cob
culture medium
edible fungi
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Pending
Application number
CN202110016055.1A
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Chinese (zh)
Inventor
谌金吾
王杰
顾泽谋
吴国平
盘祖香
麻智芳
赵发彪
季琳琪
孙厚静
谢永
王明鸾
李膳利
陆兰芳
郭显会
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Southeast Guizhou Julongtan Ecological Fishery Co ltd
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Southeast Guizhou Julongtan Ecological Fishery Co ltd
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Priority to CN202110016055.1A priority Critical patent/CN112790066A/en
Publication of CN112790066A publication Critical patent/CN112790066A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost

Abstract

The invention relates to a method for preparing a straw rotting type edible fungus culture medium by using pure corncobs, which comprises the following steps: the method comprises the following steps of preparing corncobs, crushing the corncobs, soaking/cooking the corncobs, and supplementing water to the corncobs. The culture medium for straw rotting type edible fungi prepared by the method has the advantages of wide raw material source, low price, low technical level requirement and wide application, and can be applied to the raw material of the stock seeds, the cultivated seeds and the culture medium of straw rotting type edible fungi such as stropharia rugoso-annulata, agaricus bisporus, straw mushrooms, agaricus blazei and the like. The strain prepared by the method has strong hypha and large fruiting amount, and little strain debris is generated during sowing.

Description

Method for preparing straw rotting type edible fungus culture medium by using pure corncobs
Technical Field
The invention relates to the field of edible fungus cultivation, in particular to a method for cultivating straw rotting type edible fungus matrix by using pure corncobs.
Background
The edible fungus is a large-scale fungus, and the fruiting body of the edible fungus can be eaten by human beings or used as a medicine. At present, more than 90 kinds of edible fungi can be artificially cultivated or semi-artificially cultivated. According to the nutrition mode of the edible fungi, the edible fungi can be divided into 3 types of saprophytic, symbiotic and parasitic edible fungi, wherein hyphae decompose and assimilate dead plant residues by secreting various extracellular enzymes, nutrient edible fungi obtained from the decomposition and assimilation are called saprophytic type edible fungi, and most of the edible fungi cultivated artificially at present belong to saprophytic type edible fungi. The saprophytic edible fungi can be divided into wood-saprophytic edible fungi and grass-saprophytic edible fungi, the wood-saprophytic edible fungi have strong capability of decomposing lignin and wood fiber, the saprophytic edible fungi are mainly saprophytic wood, and the main wood-saprophytic edible fungi at present comprise agaric, mushroom, lucid ganoderma and the like; the straw rotting type edible fungi have poor or no capability of decomposing wood fiber and lignin, and mainly depend on degrading the nutrition of the herbaceous plant residues to rot the living stropharia rugoso-annulata, straw mushrooms, agaricus bisporus, agaricus blazei murrill and the like. The grass rotting type edible fungus strain is prepared by mixing rice husk, cotton seed husk, rice straw powder, miscellaneous wood flour and the like according to a certain proportion and adding some trace elements in an auxiliary manner and stirring. For example, the stropharia rugoso-annulata cultivated by rice husk and pure straw, the stropharia rugoso-annulata cultivated by beet leaf and the like; the rice straw and cow dung are used for cultivating the agaricus bisporus, the coprinus comatus, the agaricus blazei murill and the like. The pure corncobs are directly and simply used for cultivating the straw rotting type edible fungi, and related reports are not found.
Disclosure of Invention
The invention solves the problem of comprehensive utilization of corncobs and the problem of raw material sources of straw rotting type edible fungi. According to the nutrition contained in the corncobs, the growth nutrition requirements of the straw rotting type edible fungi can be completely met. The method can break the prior tedious operation of cultivating the straw rotting type edible fungi by utilizing the crop straws and adding other nutrient materials, and is more beneficial to popularization.
The technical scheme for solving the technical problems is as follows: a breeding method for improving the survival rate and the fighting capacity of fighting chickens comprises the following steps:
s1 preparation of corncob in the first step. When the straw rotting type edible fungi are cultivated, only corncobs are needed to be prepared, and other auxiliary materials are not needed to be prepared. Selecting corn cob which is produced in the same year and has no mildew.
S2 second step of corncob crushing. Crushing the prepared corncobs into corncob fragments and corncob powder of 1-2 cm, 1 mm-1 cm and less than 1 mm, and crushing the corncob fragments of 3 sizes: corn pellet dices with diameter less than or equal to 1 mm (10-15%), diameter less than or equal to 1 cm (15-20%), diameter between 1 cm and 2 cm (65-75%), and mixing.
S3 soaking/cooking corncob in the third step.
And (3) putting the corncobs of 3 specifications obtained by crushing the S2 into a bag, soaking the corncobs in clean water, or 3-5% of quicklime water for 3-7 days, or steaming the corncobs for 30 minutes, wherein water is required to be added for soaking or steaming until the corncobs just submerge.
S4 the fourth step is to replenish water into the corncobs. Randomly grabbing the soaked or steamed corncob fragments by a hand to tightly hold the corncob fragments with strength, and if continuous water drops can not be formed to fall, adding water to the corncob fragments according to the condition that the corncob fragments can have continuous water drops to fall; if a light grip is required to allow successive drops to fall, the relatively dry corn cob is increased to neutralize the moisture.
On the basis of the technical scheme, the invention can be further improved as follows.
Furthermore, in the step S2, the corncobs can be crushed into pieces with the diameter of less than 1 cm, the corncobs account for about 40% of the total corncobs, and the corncobs with the diameter of 2-3 cm account for 60%.
The beneficial effect of adopting the further scheme is that: the corncob crushing process is reduced, the corncob crushing process is reduced from 3 specifications to 2 specifications, and the labor cost is saved.
Drawings
FIG. 1 is a schematic diagram of a pure corn culture medium inoculated with stropharia rugoso-annulata cultivars of the present invention with strong hypha growth;
FIG. 2 is a schematic diagram showing the strong and dense hypha growth of stropharia rugoso-annulata cultivars prepared from pure corn culture medium inoculated with stropharia rugoso-annulata stock seeds according to the invention
Detailed Description
The principles and features of the present invention are described below in conjunction with the accompanying fig. 1-2, which are provided as examples to illustrate the invention and not to limit the scope of the invention.
Example 1
A method for preparing a straw rotting type edible fungus culture medium by using pure corncobs comprises the following steps:
s1: preparing 100 kilograms of fresh corncobs produced in the current year.
S2: the corn cob is crushed into 10 kg of corn cob powder with the block diameter less than or equal to 1 mm, 20 kg of corn cob blocks with the block diameter less than or equal to 1 cm and 70 kg of corn cob blocks with the block diameter of 1 cm-2 cm, and the components of the corn cob blocks with 3 specifications are mixed.
S3: and adding 120 kg of water into the crushed and mixed corncob pieces crushed in the S2 to ensure that the corncob pieces are immersed in the water, heating and boiling the corncob pieces, and keeping the corncob pieces boiled for 30 minutes.
S4: the steamed corn cob is gripped by a hand and is tightly held, if continuous water drops, the straw rotting type edible fungi can be directly inoculated, such as the cultivation culture of Stropharia rugosoannulata (as shown in figure 1, pure corn cob is inoculated to the Stropharia rugosoannulata, and the hypha grows vigorously and vigorously).
Example 2
A method for preparing a straw rotting type edible fungus culture medium by using pure corncobs comprises the following steps:
s1: preparing 100 kilograms of fresh corncobs produced in the current year.
S2: the corn cob is crushed into 40 kg of corn cob blocks with the block diameter less than or equal to 1 cm and 60 kg of corn cob blocks with the block diameter of 1-2 cm, and the components of the corn cob blocks with 2 specifications are mixed.
S3: and (3) adding 120 kg of water into the crushed and mixed corncob blocks crushed in the step (S2) to ensure that the corncob blocks are immersed in the water, and then soaking the corncob blocks for 5 days at normal temperature.
S4: and (3) gripping the corn cobs soaked in the S3, gripping the corn cobs tightly, and directly inoculating straw rotting type edible fungi if continuous water drops drop, such as inoculating stropharia rugosoannulata cultivation cultivars (as shown in figure 2, inoculating original stropharia rugosoannulata seeds with pure corn cobs to prepare stropharia rugosoannulata cultivars, wherein hypha grows vigorously and vigorously).
The fingers are firmly held by the corn cob which is soaked or cooked, and discontinuous water drops drip from the finger gaps.
Other grass rotting type edible mushrooms, such as agaricus bisporus, volvariella volvacea, agaricus blazei murill and the like, cultivation seeds and raw materials of cultivation substrates are inoculated according to the methods of the examples 1 and 2, hyphae can grow robustly and are dense.
The above-described embodiments are not intended to limit the present invention, and any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims (4)

1. The invention relates to a method for preparing a culture medium of straw rotting type edible fungi by utilizing pure corncobs,
the method comprises the following steps:
s1 preparation of corncob in the first step.
Selecting corn cob without mildew and impurities, preferably corn cob produced in the current year.
S2 second step of corncob crushing.
Corncobs are crushed into 3 sizes: corncob meal with the diameter less than or equal to 1 mm accounts for 10-15% of the total corncobs; corn pellet D with diameter less than or equal to 1 cm accounts for 15-20% of total corn cob; the corn pellet pieces with the diameter of 1-2 cm account for 65-75% of the total corn core.
S3 soaking/cooking corncob in the third step.
And (3) putting the corncobs of 3 specifications obtained by crushing the S2 into a bag, soaking the corncobs in clean water, or 3-5% of quicklime water for 3-7 days, or steaming the corncobs for 30 minutes, wherein water is required to be added for soaking or steaming until the corncobs just submerge.
S4 the fourth step is to replenish water into the corncobs. And (3) soaking or cooking the corncobs, and ensuring that the water content is over 75 percent, namely, the soaked or cooked corncobs are held by fingers, and discontinuous water drops drip from finger joints.
The culture medium for straw rotting type edible fungi prepared by the method has the advantages of wide raw material source, low price, low technical level requirement and wide application, and can be applied to the raw material of the stock seeds, the cultivated seeds and the culture medium of straw rotting type edible fungi such as stropharia rugoso-annulata, agaricus bisporus, straw mushrooms, agaricus blazei and the like. If the strain is used as a raw material for culturing the grass rotting type original seeds, the strain can be directly inoculated with the mother seeds; if the strain is used as a cultivation seed raw material, the stock can be directly inoculated; if the medium is used as a culture medium, the cultivated species of the edible fungi can be directly sown.
2. The method as claimed in claim 1, wherein the corncob is used to prepare the culture medium for straw rotting type edible fungi, which can be directly used as the raw material for the culture medium, the stock culture and the cultivation medium for all the straw rotting type edible fungi which can be cultivated on a large scale at present, such as stropharia rugoso-annulata, straw mushroom, agaricus blazei murrill, agaricus bisporus, etc.
3. The method for preparing culture medium of straw rotting type edible fungi from pure corncob according to claim 1, wherein the corncob is crushed into 3 sizes in different percentage, that is, the corncob is crushed into corncob meal with diameter less than or equal to 1 mm, which accounts for 10-15% of the total corncob; crushing the corn cob into corn cob pieces with the diameter less than or equal to 1 cm, wherein the corn cob pieces account for 15-20% of the total corn cob; the corn cob is crushed into corn cob blocks with the diameter of 1 cm-2 cm, and the corn cob blocks account for 65-75% of the total corn cob. The method has the advantages that the raw materials can be ensured to have thickness and fineness after being fully stirred, the original enough variable area and the growth nutrition and space of the straw rotting type edible fungus hyphae are ensured, certain gaps among the corncob substrates can be fully ensured, the ventilation and the air permeability are ensured, and the oxygen required by the hyphae growth is preserved.
4. The method for preparing culture medium of straw rotting type edible fungi according to claim 1, wherein the corncob is the product of corn cob after corn kernel is removed, and can be called as "corn cob", "corn ball", "corn long", "corn cob", etc. and the nutrient components are as follows: the corn cob contains 54.5% of sugar, 2.2% of crude protein, 0.4% of crude fat, 29.7% of crude fiber and 1.2% of mineral substances, and the nutrition contained in the corn cob can meet the nutritional requirements of the growth of all the hypha of the grass rotting edible fungi which can be cultivated on a large scale at present.
CN202110016055.1A 2021-01-07 2021-01-07 Method for preparing straw rotting type edible fungus culture medium by using pure corncobs Pending CN112790066A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103348870A (en) * 2013-07-22 2013-10-16 何寒 Method for using integral corncobs as raw material for preparing agaric fungus bag
CN103641639A (en) * 2013-12-24 2014-03-19 齐鲁工业大学 Edible straw rotting fungus culture medium and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103348870A (en) * 2013-07-22 2013-10-16 何寒 Method for using integral corncobs as raw material for preparing agaric fungus bag
CN103641639A (en) * 2013-12-24 2014-03-19 齐鲁工业大学 Edible straw rotting fungus culture medium and preparation method thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
李元西: "整玉米芯栽培食用菌技术", 《四川农业科技》 *
段双路: "整玉米轴玉米地套种草菇技术", 《食用菌》 *
王娌莉等: "新鲜甜玉米芯在草菇栽培与菌种制作上的应用研究", 《广东农业科学》 *
闫振教: "玉米芯生料栽培平菇技术", 《食用菌》 *

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