CN112778062A - Functional organic fertilizer containing humic acid - Google Patents
Functional organic fertilizer containing humic acid Download PDFInfo
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- CN112778062A CN112778062A CN202110030692.4A CN202110030692A CN112778062A CN 112778062 A CN112778062 A CN 112778062A CN 202110030692 A CN202110030692 A CN 202110030692A CN 112778062 A CN112778062 A CN 112778062A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to the technical field of organic fertilizer production, and discloses a functional organic fertilizer containing humic acid, which comprises the following components: 80-90 parts of animal waste, 40-50 parts of bran, 20-25 parts of straw, 10-12 parts of sawdust, 6-8 parts of humic acid bacteria agent, 0.5-1 part of first microbial bacterium complexing agent, 1-1.5 parts of second microbial bacterium complexing agent, a plurality of parts of urea and a plurality of parts of calcium superphosphate. The organic fertilizer consists of animal wastes, bran, straws, sawdust, a humic acid microbial inoculum, a first microbial bacterium complexing agent, a second microbial bacterium complexing agent, a plurality of parts of urea and a plurality of parts of calcium superphosphate, so that the organic matter content of soil can be increased, the soil can be loosened, the soil structure can be improved, the influence on the environment is small, the effective period of the fertility is long, the fertility is lasting and mild, the effect of protecting the environment can be achieved, and meanwhile, the total nitrogen content, the total phosphorus content and the total potassium content in the organic fertilizer are high, so that nutrients required by crops can be provided, and the biological yield is improved.
Description
Technical Field
The invention relates to the technical field of organic fertilizer production, in particular to a functional organic fertilizer containing humic acid.
Background
The organic fertilizer is a traditional fertilizer in China, can provide rich organic matters, nitrogen, phosphorus, potassium and various trace elements for the growth and development of plants, supplements soil nutrients at any time, improves the soil structure, fertilizes the soil, also has the functions of eliminating harmful substances poisoned in the soil and the like, but also has the defects of lasting and slow fertilizer effect, dirty and smelly properties, insanitation, low nutrient content, large volume, inconvenient use and the like.
Chinese patent discloses a biological humic acid compound fertilizer and a manufacturing method thereof (No. CN 1279004C), the technology of the patent has the long-acting property of biological organic fertilizer and the special effect of improving soil, and the fertilizer is an ideal novel fertilizer for developing green agricultural products, but the fertilizer has poor soil improvement effect and low nutrient content.
Disclosure of Invention
The invention aims to provide a functional organic fertilizer containing humic acid to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a functional organic fertilizer containing humic acid comprises the following components: 80-90 parts of animal waste, 40-50 parts of bran, 20-25 parts of straw, 10-12 parts of sawdust, 6-8 parts of humic acid bacteria agent, 0.5-1 part of first microbial bacterium complexing agent, 1-1.5 parts of second microbial bacterium complexing agent, a plurality of parts of urea and a plurality of parts of calcium superphosphate, wherein the preparation process comprises the following steps:
s1, mixing 150-200 parts of animal wastes, 40-50 parts of bran, 20-25 parts of straws and 10-12 parts of sawdust, and adding a plurality of parts of urea to adjust the C/N ratio of the mixture, so that the C/N ratio is kept between 30:1 and 35: 1;
s2, adding 0.5-1 part of the first microbial bacterium complexing agent into the mixture obtained in the step S1, stirring, and adding a plurality of parts of calcium superphosphate to adjust the pH value of the mixture in the stirring process so as to control the pH value to be 7.0-7.5;
s3, stacking the mixture obtained in the step S3 in a ventilated environment, fermenting and decomposing the mixture, wherein the highest temperature is controlled to be 50-55 ℃, and the moisture is controlled to be 55-65%;
and S4, turning the piles once every 48 hours to control the oxygen content of the piles to be 16-20%, fermenting and decomposing for 20 days, adding 6-8 parts of humic acid bacteria and 1-1.5 parts of second microorganism bacterium complexing agent after the fermentation and the decomposition are finished, uniformly mixing and stirring, and drying to obtain the product organic fertilizer.
As a further scheme of the invention: the humic acid microbial inoculum consists of the following components: 200-250 parts of peat, 2-3 parts of fermentation sugar, 2-3 parts of corn starch, 1-2 parts of flocculant, 30-35 parts of NaOH solution and a plurality of parts of HCl solution.
As a still further scheme of the invention: the preparation process of the humic acid microbial inoculum comprises the following steps:
s11, soaking 200-250 parts of peat in 30-35 parts of NaOH solution, grinding and sieving, wherein the granularity is controlled to be 100-120 meshes;
s12, adding 2-3 parts of fermentation sugar and 2-3 parts of corn starch, heating in a water bath to fully dissolve the fermentation sugar and the corn starch, adding 1-2 parts of flocculating agent, performing centrifugal separation to obtain filtrate, washing residues with clear water, and removing impurities to obtain washing liquid;
s13, mixing the filtrate with the washing liquid, adding a plurality of parts of HCl solution, adjusting the pH value to 1.5-2.0, uniformly stirring, performing reduced pressure suction filtration after stirring, and drying the solid phase to obtain the humic acid microbial inoculum.
As a still further scheme of the invention: the first microbial agent is composed of the following components: bacillus subtilis, aspergillus oryzae, thermophilic sporotrichum, trichoderma viride, a liquid agar culture medium and wheat bran.
As a still further scheme of the invention: the culture process of the first microbial inoculant comprises the following steps:
s21, respectively putting the bacillus subtilis, the aspergillus oryzae, the thermophilic sporotrichum and the trichoderma viride into independent liquid agar culture media for culture according to the ratio of 1:2, wherein the culture temperature is (30 +/-0.5) DEG C, then carrying out centrifugal stirring for 5-8 min at the centrifugal rotation speed of 2800-3200 rpm, and after stirring is finished, taking the filtrate for culture for 48h to respectively obtain four strain liquid of the bacillus subtilis, the aspergillus oryzae, the thermophilic sporotrichum and the trichoderma viride;
s22, respectively putting the four strain solutions into a fermentation tank for amplification culture for 48h to obtain fermentation liquor, mixing the fermentation liquor with wheat bran according to the ratio of 1:2, fermenting for 48h again, and drying to obtain four solid microbial agents, namely bacillus subtilis, aspergillus oryzae, thermophilic sporotrichum and trichoderma viride;
s23, uniformly mixing the four solid microbial agents of the bacillus subtilis, the aspergillus oryzae, the thermophilic sporotrichum and the trichoderma viride according to the proportion of 1:2:2:1 to obtain the microbial agent complex.
As a still further scheme of the invention: the second microbial agent is composed of the following components: azotobacteria, azotobacteria chroococcum, bacillus megaterium, bacillus mucilaginosus and liquid agar culture medium.
As a still further scheme of the invention: the culture process of the second microbial inoculant comprises the following steps:
s31, respectively putting nitrogen-fixing bacillus, azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus into independent liquid agar culture media according to the ratio of 1:2 for culture at the temperature of (30 +/-0.5) DEG C, then carrying out centrifugal stirring for 5-8 min at the centrifugal rotation speed of 1800-2200 rpm, and after stirring is finished, taking filtrate for fermentation culture for 48h to obtain four kinds of fermentation liquor;
s32, respectively reaching 10 thalli of the four fermentation liquors obtained in the step S318～109After cfu/L, respectively inoculating the bacillus subtilis and the bacillus mucilaginosus to wheat bran according to the proportion of 1:2, fermenting for 48 hours again, and drying to obtain four solid microbial agents of nitrogen-fixing bacillus, azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus;
s33, mixing the four solid microbial agents of the nitrogen-fixing bacillus, the azotobacter chroococcum, the bacillus megaterium and the colloidal bacillus according to the proportion of 1:1:2:2 to obtain the second microbial agent.
Compared with the prior art, the invention has the beneficial effects that:
the invention consists of animal wastes, bran, straws, sawdust, humic acid bacteria, a first microbial bacteria complexing agent, a second microbial bacteria complexing agent, a plurality of parts of urea and a plurality of parts of calcium superphosphate, wherein the first microbial bacteria complexing agent consists of bacillus subtilis, aspergillus oryzae, thermophilic sporotrichum, trichoderma viride, a liquid agar culture medium and wheat bran, the second microbial bacteria complexing agent consists of nitrogen fixing bacillus, azotobacter chroococcum, bacillus megaterium, bacillus mucilaginosus and a liquid agar culture medium, thereby increasing the organic matter content of soil, loosening the soil, improving the soil structure, having small influence on the environment, having long effective period of fertility, lasting and mild fertility, further playing a role in protecting the environment, having higher total nitrogen content, total phosphorus content and total potassium content in organic fertilizer, thereby providing nutrients required by crops, improve the biological yield and the nutritive value of crops.
Detailed Description
In the embodiment of the invention, the functional organic fertilizer containing humic acid comprises the following components: 80-90 parts of animal waste, 40-50 parts of bran, 20-25 parts of straw, 10-12 parts of sawdust, 6-8 parts of humic acid bacteria agent, 0.5-1 part of first microbial bacterium complexing agent, 1-1.5 parts of second microbial bacterium complexing agent, a plurality of parts of urea and a plurality of parts of calcium superphosphate, wherein the preparation process comprises the following steps:
s1, mixing 150-200 parts of animal wastes, 40-50 parts of bran, 20-25 parts of straws and 10-12 parts of sawdust, and adding a plurality of parts of urea to adjust the C/N ratio of the mixture, so that the C/N ratio is kept between 30:1 and 35: 1;
s2, adding 0.5-1 part of the first microbial bacterium complexing agent into the mixture obtained in the step S1, stirring, and adding a plurality of parts of calcium superphosphate to adjust the pH value of the mixture in the stirring process so as to control the pH value to be 7.0-7.5;
s3, stacking the mixture obtained in the step S3 in a ventilated environment, fermenting and decomposing the mixture, wherein the highest temperature is controlled to be 50-55 ℃, and the moisture is controlled to be 55-65%;
and S4, turning the piles once every 48 hours to control the oxygen content of the piles to be 16-20%, fermenting and decomposing for 20 days, adding 6-8 parts of humic acid bacteria and 1-1.5 parts of second microorganism bacterium complexing agent after the fermentation and the decomposition are finished, uniformly mixing and stirring, and drying to obtain the product organic fertilizer.
Preferably, the humic acid bacteria agent consists of the following components: 200-250 parts of peat, 2-3 parts of fermentation sugar, 2-3 parts of corn starch, 1-2 parts of flocculant, 30-35 parts of NaOH solution and a plurality of parts of HCl solution.
Preferably, the preparation process of the humic acid microbial inoculum comprises the following steps:
s11, soaking 200-250 parts of peat in 30-35 parts of NaOH solution, grinding and sieving, wherein the granularity is controlled to be 100-120 meshes;
s12, adding 2-3 parts of fermentation sugar and 2-3 parts of corn starch, heating in a water bath to fully dissolve the fermentation sugar and the corn starch, adding 1-2 parts of flocculating agent, performing centrifugal separation to obtain filtrate, washing residues with clear water, and removing impurities to obtain washing liquid;
s13, mixing the filtrate with the washing liquid, adding a plurality of parts of HCl solution, adjusting the pH value to 1.5-2.0, uniformly stirring, performing reduced pressure suction filtration after stirring, and drying the solid phase to obtain the humic acid microbial inoculum.
Preferably, the first microbial inoculant consists of: bacillus subtilis, aspergillus oryzae, thermophilic sporotrichum, trichoderma viride, a liquid agar culture medium and wheat bran.
Preferably, the process for culturing the first microbial inoculant comprises the following steps:
s21, respectively putting the bacillus subtilis, the aspergillus oryzae, the thermophilic sporotrichum and the trichoderma viride into independent liquid agar culture media for culture according to the ratio of 1:2, wherein the culture temperature is (30 +/-0.5) DEG C, then carrying out centrifugal stirring for 5-8 min at the centrifugal rotation speed of 2800-3200 rpm, and after stirring is finished, taking the filtrate for culture for 48h to respectively obtain four strain liquid of the bacillus subtilis, the aspergillus oryzae, the thermophilic sporotrichum and the trichoderma viride;
s22, respectively putting the four strain solutions into a fermentation tank for amplification culture for 48h to obtain fermentation liquor, mixing the fermentation liquor with wheat bran according to the ratio of 1:2, fermenting for 48h again, and drying to obtain four solid microbial agents, namely bacillus subtilis, aspergillus oryzae, thermophilic sporotrichum and trichoderma viride;
s23, uniformly mixing the four solid microbial agents of the bacillus subtilis, the aspergillus oryzae, the thermophilic sporotrichum and the trichoderma viride according to the proportion of 1:2:2:1 to obtain the microbial agent complex.
Preferably, the second microbial inoculant consists of: azotobacteria, azotobacteria chroococcum, bacillus megaterium, bacillus mucilaginosus and liquid agar culture medium.
Preferably, the process for culturing the second microbial inoculant comprises the following steps:
s31, respectively putting nitrogen-fixing bacillus, azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus into independent liquid agar culture media according to the ratio of 1:2 for culture at the temperature of (30 +/-0.5) DEG C, then carrying out centrifugal stirring for 5-8 min at the centrifugal rotation speed of 1800-2200 rpm, and after stirring is finished, taking filtrate for fermentation culture for 48h to obtain four kinds of fermentation liquor;
s32, respectively reaching 10 thalli of the four fermentation liquors obtained in the step S318～109After cfu/L, respectively inoculating the bacillus subtilis and the bacillus mucilaginosus to wheat bran according to the proportion of 1:2, fermenting for 48 hours again, and drying to obtain four solid microbial agents of nitrogen-fixing bacillus, azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus;
s33, mixing the four solid microbial agents of the nitrogen-fixing bacillus, the azotobacter chroococcum, the bacillus megaterium and the colloidal bacillus according to the proportion of 1:1:2:2 to obtain the second microbial agent.
To better illustrate the technical effect of the present invention, it is illustrated by the following tests:
taking the organic fertilizer obtained by the invention as an embodiment, randomly purchasing two organic fertilizers on the market as a first comparative example and a second comparative example;
respectively carrying out sensory inspection and distilled naugh spectrophotometry detection on 10g of organic fertilizers in the examples and the comparative examples I and II, respectively, carrying out sensory inspection on the odor and the appearance of the organic fertilizers, and detecting NH of the organic fertilizers by the distilled naugh spectrophotometry3N content (unit: mg/L), the results of the tests are given in Table 1 below:
table 1, examples, comparative example A and comparative example B
| Name of item | Examples | Comparative example 1 | Comparative example No. two |
| Smell and appearance | No odor and loose structure | Slightly smelly and loose structure | No odor and dense structure |
| NH3Content of-N | 0.73 | 1.19 | 1.22 |
From the analysis in table 1 it can be derived: from the odor and appearance: the organic fertilizer in the embodiment has no odor and loose appearance structure, while the organic fertilizer in the comparative example I has slight odor and loose appearance structure, the organic fertilizer in the comparative example II has no odor and dense structure, and NH of the organic fertilizer in the embodiment can be obtained by a distillation nano spectrophotometry3NH content of-N is significantly lower than that of organic fertilizer in comparative example one and comparative example two3The ammonia content is lower in the secondary demonstration embodiment because the ammonia is the main component of the odor of the excrement, so that the ammonia content is better in the ammonia reducing and deodorizing effects, and meanwhile, the structure is loose, and the absorption of crops is facilitated.
Secondly, 10g of organic fertilizer in each of the examples and the comparative examples is taken again, and effective viable count (cfu) (unit: hundred million/g), organic matter content (calculated by dry basis), escherichia coli count (unit: one/g), ascarid egg death rate (%), and total nitrogen (calculated by N) are respectively carried out) Content (unit mg/kg), total phosphorus (in P)2O5In mg/kg), total potassium (in K)2O) content (in mg/kg), the test results are given in table 2 below:
table 2, examples, comparative example A and comparative example B
| Name of item | Examples | Comparative example 1 | Comparative example No. two |
| Effective viable count (cfu) | 1.25 | 0.43 | 0.51 |
| Organic matter content | 36.7% | 28.5% | 29.1% |
| Number of Escherichia coli | 11 | 24 | 22 |
| Death rate of ascarid eggs | 100% | 96% | 98% |
| Total nitrogen (in N) content | 4.62% | 3.18% | 3.75% |
| Total phosphorus (in P)2O5Meter) content of | 0.79% | 0.49% | 0.42% |
| Total potassium (in K)2Calculated as O) content | 3.17% | 2.56% | 2.26% |
From the analysis in table 2 it can be derived: firstly, the effective viable count of the organic fertilizer in the embodiment is obviously higher than that of the organic fertilizer in the first comparative example and the second comparative example, and the number of escherichia coli of the organic fertilizer in the embodiment is obviously lower than that of the organic fertilizer in the first comparative example and the second comparative example, so that the following results can be obtained: the organic fertilizer in the embodiment can increase the organic matter content of soil, loosen the soil and improve the soil structure, has small influence on the environment, has long effective period of fertility and lasting and mild fertility, and further can play a role in protecting the environment;
secondly, the total nitrogen (calculated as N) content and total phosphorus (calculated as P) of the organic fertilizer in the example2O5Calculated as K) content and total potassium (in K)2Calculated as O) contents are higher than those of the corresponding elements in comparative example one and comparative example two, so that it can be concluded that: in the embodiment, the nutrient content of the organic fertilizer is high, so that nutrients required by crops can be provided, the biological yield is increased, and the nutritional value of the crops is improved.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention are equivalent to or changed within the technical scope of the present invention.
Claims (7)
1. A functional organic fertilizer containing humic acid is characterized by comprising the following components: 80-90 parts of animal waste, 40-50 parts of bran, 20-25 parts of straw, 10-12 parts of sawdust, 6-8 parts of humic acid bacteria agent, 0.5-1 part of first microbial bacterium complexing agent, 1-1.5 parts of second microbial bacterium complexing agent, a plurality of parts of urea and a plurality of parts of calcium superphosphate, wherein the preparation process comprises the following steps:
s1, mixing 150-200 parts of animal wastes, 40-50 parts of bran, 20-25 parts of straws and 10-12 parts of sawdust, and adding a plurality of parts of urea to adjust the C/N ratio of the mixture, so that the C/N ratio is kept between 30:1 and 35: 1;
s2, adding 0.5-1 part of the first microbial bacterium complexing agent into the mixture obtained in the step S1, stirring, and adding a plurality of parts of calcium superphosphate to adjust the pH value of the mixture in the stirring process so as to control the pH value to be 7.0-7.5;
s3, stacking the mixture obtained in the step S3 in a ventilated environment, fermenting and decomposing the mixture, wherein the highest temperature is controlled to be 50-55 ℃, and the moisture is controlled to be 55-65%;
and S4, turning the piles once every 48 hours to control the oxygen content of the piles to be 16-20%, fermenting and decomposing for 20 days, adding 6-8 parts of humic acid bacteria and 1-1.5 parts of second microorganism bacterium complexing agent after the fermentation and the decomposition are finished, uniformly mixing and stirring, and drying to obtain the product organic fertilizer.
2. The functional organic fertilizer containing humic acid according to claim 1, wherein the humic acid bacterial agent consists of the following components: 200-250 parts of peat, 2-3 parts of fermentation sugar, 2-3 parts of corn starch, 1-2 parts of flocculant, 30-35 parts of NaOH solution and a plurality of parts of HCl solution.
3. The functional organic fertilizer containing humic acid according to claim 2, wherein the preparation process of the humic acid microbial inoculum comprises the following steps:
s11, soaking 200-250 parts of peat in 30-35 parts of NaOH solution, grinding and sieving, wherein the granularity is controlled to be 100-120 meshes;
s12, adding 2-3 parts of fermentation sugar and 2-3 parts of corn starch, heating in a water bath to fully dissolve the fermentation sugar and the corn starch, adding 1-2 parts of flocculating agent, performing centrifugal separation to obtain filtrate, washing residues with clear water, and removing impurities to obtain washing liquid;
s13, mixing the filtrate with the washing liquid, adding a plurality of parts of HCl solution, adjusting the pH value to 1.5-2.0, uniformly stirring, performing reduced pressure suction filtration after stirring, and drying the solid phase to obtain the humic acid microbial inoculum.
4. The functional organic fertilizer containing humic acid according to claim 1, wherein the first microbial inoculant consists of: bacillus subtilis, aspergillus oryzae, thermophilic sporotrichum, trichoderma viride, a liquid agar culture medium and wheat bran.
5. The functional organic fertilizer containing humic acid according to claim 4, wherein the cultivation process of the first microbial compound agent comprises the following steps:
s21, respectively putting the bacillus subtilis, the aspergillus oryzae, the thermophilic sporotrichum and the trichoderma viride into independent liquid agar culture media for culture according to the ratio of 1:2, wherein the culture temperature is (30 +/-0.5) DEG C, then carrying out centrifugal stirring for 5-8 min at the centrifugal rotation speed of 2800-3200 rpm, and after stirring is finished, taking the filtrate for culture for 48h to respectively obtain four strain liquid of the bacillus subtilis, the aspergillus oryzae, the thermophilic sporotrichum and the trichoderma viride;
s22, respectively putting the four strain solutions into a fermentation tank for amplification culture for 48h to obtain fermentation liquor, mixing the fermentation liquor with wheat bran according to the ratio of 1:2, fermenting for 48h again, and drying to obtain four solid microbial agents, namely bacillus subtilis, aspergillus oryzae, thermophilic sporotrichum and trichoderma viride;
s23, uniformly mixing the four solid microbial agents of the bacillus subtilis, the aspergillus oryzae, the thermophilic sporotrichum and the trichoderma viride according to the proportion of 1:2:2:1 to obtain the microbial agent complex.
6. The functional organic fertilizer containing humic acid according to claim 1, wherein the second microbial inoculant consists of the following components: azotobacteria, azotobacteria chroococcum, bacillus megaterium, bacillus mucilaginosus and liquid agar culture medium.
7. The functional organic fertilizer containing humic acid according to claim 6, wherein the culturing process of the second microbial compound agent comprises the following steps:
s31, respectively putting nitrogen-fixing bacillus, azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus into independent liquid agar culture media according to the ratio of 1:2 for culture at the temperature of (30 +/-0.5) DEG C, then carrying out centrifugal stirring for 5-8 min at the centrifugal rotation speed of 1800-2200 rpm, and after stirring is finished, taking filtrate for fermentation culture for 48h to obtain four kinds of fermentation liquor;
s32, respectively reaching 10 thalli of the four fermentation liquors obtained in the step S318～109After cfu/L, respectively inoculating the bacillus subtilis and the bacillus mucilaginosus to wheat bran according to the proportion of 1:2, fermenting for 48 hours again, and drying to obtain four solid microbial agents of nitrogen-fixing bacillus, azotobacter chroococcum, bacillus megaterium and bacillus mucilaginosus;
s33, mixing the four solid microbial agents of the nitrogen-fixing bacillus, the azotobacter chroococcum, the bacillus megaterium and the colloidal bacillus according to the proportion of 1:1:2:2 to obtain the second microbial agent.
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| CN113480360A (en) * | 2021-07-27 | 2021-10-08 | 海南金雨丰生物工程有限公司 | Humic acid fertilizer and preparation method thereof |
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