CN112760357B - Method for detecting microorganisms in swimming pool water - Google Patents
Method for detecting microorganisms in swimming pool water Download PDFInfo
- Publication number
- CN112760357B CN112760357B CN202110167455.2A CN202110167455A CN112760357B CN 112760357 B CN112760357 B CN 112760357B CN 202110167455 A CN202110167455 A CN 202110167455A CN 112760357 B CN112760357 B CN 112760357B
- Authority
- CN
- China
- Prior art keywords
- water
- mixing
- swimming pool
- sampling
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Sustainable Development (AREA)
- Immunology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The application relates to a swimming pool water microorganism detection method, and belongs to the technical field of water quality detection. Which comprises the following steps: 1) collecting sample water through water collecting equipment; 1.1) collecting the swimming pool water at each sampling point; 1.2) mixing the collected swimming pool water at each sampling point; 1.3) extracting sample water from the mixed swimming pool water; 2) preparing a reserve culture medium; 3) preparing a plate culture medium; 4) preparing lactose peptone culture solution; 5) sterilizing the filter membrane; 6) sterilizing a filter; 7) filtering the water sample; 8) and culturing; 9) picking the bacterial colony to perform gram staining and microscopic examination; 10) inoculating gram-negative bacillus-free bacillus into lactose peptone culture solution, and culturing for 48h in an environment with the temperature of 36 +/-1 ℃, wherein a person producing acid and gas is proved to be positive in coliform; 11) the number of colonies on the filter which were confirmed to be coliform colonies was counted. This application has the convenient effect of daily detection of swimming pool water.
Description
Technical Field
The application relates to the field of water quality detection, in particular to a method for detecting microorganisms in swimming pool water.
Background
The measurement of microorganisms in water is of great significance in ensuring water source safety and controlling infectious diseases, and is also an important index for evaluating water quality conditions, particularly the swimming pool needs to carry out microorganism detection on swimming pool water regularly, particularly the detection of coliform group, wherein the coliform group refers to a group of aerobic and facultative anaerobic gram-negative bacillus-free bacteria which can ferment lactose, produce acid and produce gas and are cultured for 24 hours in the environment of 36 +/-1 ℃.
The measurement of microorganisms in water is carried out by a plate counting method, namely, after a sample to be measured is properly diluted, the microorganisms in the sample are fully dispersed into single cells, a certain amount of diluted sample liquid is taken to be coated on a plate, each single cell grows and reproduces to form a colony visible to naked eyes after being cultured, namely, one single colony represents one single cell in an original sample, the colony number is counted, and the bacteria-containing number in the sample can be converted according to the dilution multiple and the sampling inoculation amount. And a filtration method in which a filter membrane having an appropriate pore size is placed in a filter, a sample is filtered, microorganisms are retained on the surface of the membrane by the filter membrane, a growth inhibitor for microorganisms in the sample can be removed by washing the filter with sterile water after filtration, then the filter membrane is cultured on a culture medium, nutrients and metabolites are exchanged through the micropores of the filter membrane, and colonies cultured on the surface of the filter membrane can be counted and correlated with the amount of the sample.
To the correlation technique among the above-mentioned, the inventor thinks when carrying out water quality testing to the swimming pool, need confirm a plurality of sampling points at the swimming pool, detect through the appearance water to each sampling point and gather just can obtain a comparatively accurate data, waste time and energy, it is too troublesome to the daily management in pond.
Disclosure of Invention
In order to facilitate daily detection of swimming pool water, the application provides a method for detecting microorganisms in swimming pool water.
The application provides a swimming pool water microorganism detection method adopts following technical scheme:
a method for detecting microorganisms in swimming pool water, which comprises the following steps:
1) collecting sample water through water collecting equipment;
1.1) collecting the swimming pool water at each sampling point;
1.2) mixing the collected swimming pool water at each sampling point;
1.3) extracting sample water from the mixed swimming pool water;
2) preparing a reserve culture medium;
3) preparing a plate culture medium;
4) preparing lactose peptone culture solution;
5) sterilizing the filter membrane;
6) sterilizing a filter;
7) filtering the water sample;
8) and culturing;
9) picking the bacterial colony to perform gram staining and microscopic examination;
10) inoculating gram-negative bacillus-free bacillus into lactose peptone culture solution, and culturing for 48h in an environment with the temperature of 36 +/-1 ℃, wherein a person producing acid and gas is proved to be positive in coliform;
11) The number of colonies which were confirmed to be coliform groups and which had grown on the filter was counted.
Through adopting above-mentioned technical scheme, carry out mixed treatment through the water sample to each sampling point, then the swimming pool water after the extraction mixes is as the appearance water, then carries out subsequent water sample and detects, when reducing the quantity of water sample, can obtain the data that comparatively accurate can reflect the swimming pool water condition again, conveniently carries out daily detection to swimming pool water.
Optionally, each collecting point in step 1.1) collects a water sample from the middle layer to the surface layer.
Through adopting above-mentioned technical scheme, every collection point is gathered swimming pool water top layer to medial water sample for every collection point gathers the water sample more accurate, because the condition influence of swimming pool bottom of water portion is less, the swimming pool water top layer is main contact water to the part between the middle level, and the germ volume in corresponding top layer aquatic is more, such detection mode can be so that the data that detect out is more accurate, reduces the influence of swimming pool bottom layer part to the testing result.
Optionally, in step 1.1), distribution of staff in each area of the swimming pool is investigated before determining the collection points, the collection points are divided according to the staff in the area, and more collection points are arranged at the position with dense staff.
Through adopting above-mentioned technical scheme, carry out corresponding collection point according to the regional personnel distribution condition of swimming pool and divide for the setting of collection point is more reasonable, the condition of reaction swimming pool water that can be better during the detection.
Optionally, in step 1) water sampling equipment includes water sampling shell, water sampling piston, water sampling pole, hybrid piston and hybrid rod, be provided with the hybrid chamber in the water sampling shell and be located the a plurality of water sampling chamber of hybrid chamber outer lane, water sampling piston have a plurality of and one-to-one slide install in the water sampling intracavity, water sampling pole has a plurality ofly, and is a plurality of water sampling pole one-to-one is fixed in on the water sampling piston, hybrid piston slide install in the hybrid chamber is fixed the hybrid rod, the hybrid chamber is with a plurality of the water sampling chamber all communicates each other and is provided with the check valve, water sampling chamber bottom is provided with the outside hose that absorbs water of intercommunication and is provided with the check valve, the chamber bottom of hybrid chamber is provided with the outside appearance pipe of intercommunication and installs the check valve.
By adopting the technical scheme, the arranged water collecting cavities are used for correspondingly receiving the water of the swimming pool collected by each collecting point one by one, the water collecting piston is driven by the water collecting rod to move in the water collecting cavity, a vacuum environment is formed in the water collecting cavity, then the water absorbing hose absorbs the water of the swimming pool from the collecting point and sends the water into the water collecting cavity, then the mixing piston is driven by the mixing rod to move upwards in the mixing cavity, a vacuum environment is formed in the mixing cavity, thereby the water of each water sampling intracavity is inhaled the hybrid chamber and is mixed, and the play appearance pipe that the hybrid chamber bottom set up is used for delivering out the swimming pool water that mixes, and whole equipment collection is gathered some water sampling, mixes each and gathers the swimming pool water of some collection and take out functions in an organic whole such as sample water from the swimming pool aquatic of mixing, and it is more convenient to operate, and the associativity each other is better, step and loaded down with trivial details degree when further simplifying the water sampling, and the convenience carries out daily measuring to swimming pool water.
Optionally, a plurality of linkage assemblies are arranged on the water sampling shell, the linkage assemblies correspond to the water sampling rods one by one, each linkage assembly comprises a supporting rod and a linkage rod, the supporting rod is vertically fixed on the water sampling shell, the middle of each linkage rod is rotatably installed at one end, far away from the water sampling shell, of the supporting rod, one end of each linkage rod is located above the water sampling rod, and the other end of each linkage rod is located above the mixing rod.
Through adopting above-mentioned technical scheme, the one end that the trace is located water sampling rod top is used for restricting water sampling rod on the one hand and breaks away from the water sampling chamber, be used for the mixing rod to drive the water sampling rod simultaneously again and move down, the mixing chamber is followed water sampling chamber extraction swimming pool water and is adopted the chamber to push into swimming pool water to the mixing chamber and go on simultaneously, make the mixture of swimming pool water more simple and convenient, water sampling rod shifts up when extracting the swimming pool water from the swimming pool, can not receive the interference of trace, make things convenient for the collection of swimming pool water, can not receive the restriction of water sampling rod again when mixing rod moves down, make things convenient for the extrusion of appearance water.
Optionally, the mixing rod lateral wall is provided with the overhang board, trace one end is located the overhang board upside, be provided with the spliced pole on the water production pole and draw the post, the spliced pole and draw the post all with the water production pole is coaxial, just the diameter of spliced pole is less than the diameter of water production pole and draw the diameter of post, the trace other end is located draw the post with between the water production pole.
Through adopting above-mentioned technical scheme, set up the overhang board, promote trace one end through the overhang board and shift up for the diameter of mixing rod need not very big, reduces the quality and the space ratio of mixing rod, is favorable to the pulling of mixing rod, keeps the pushing action to the trace simultaneously, and the length requirement of trace is lower, is difficult to interfere between the adjacent trace more.
Optionally, install out the appearance rubber tube on going out the appearance pipe, it divides the pipe to be provided with two play appearances on going out the appearance rubber tube water sampling equipment still includes aseptic water sampling bag and stagnant water clamp, the stagnant water clamp sets up in one go out the appearance and divide on the pipe, another go out the appearance divide the pipe with aseptic sample inlet pipe cartridge of aseptic water sampling bag is fixed, stagnant water clamp is fixed with aseptic water sampling bag.
By adopting the technical scheme, the water stop clamp is firstly fixed on one sample outlet branched pipe during use, then partial sample water is extruded through the other sample outlet branched pipe, the sterile water sampling bag and the other sample outlet branched pipe are inserted and fixed, the sample water is injected, then the water stop clamp is taken down, and the residual sample water is discharged from the other sample outlet branched pipe.
Optionally, a plurality of supporting legs are arranged below the water sampling shell, brake casters are arranged below the supporting legs, a wastewater box is arranged between the supporting legs, and a supporting column for placing the sterile water sampling bag is arranged in the middle of the wastewater box.
Through adopting above-mentioned technical scheme for the removal that water sampling equipment can be convenient carries out each and gathers the collection of some swimming pools water, and the brake truckle is fixed water sampling equipment in situ, and the operation of being convenient for adopt water, unnecessary sample water is received to the waste water box, makes things convenient for post processing waste water, and the support column is used for placing aseptic water sampling bag and carries out the receipt of sample water.
Optionally, water sampling equipment still includes the flabellum, the flabellum includes a plurality of blades, the flabellum rotate install in at the bottom of the chamber of mixing chamber, mixing piston orientation the arrangement chamber that supplies the flabellum to get into is seted up to the lateral wall at the bottom of the mixing chamber, and is a plurality of the axis orientation in mixing hole the blade setting of flabellum.
Through adopting above-mentioned technical scheme, it is rotatory to drive the flabellum from the inspiratory swimming pool water in water cavity of adopting for step 1.1) and step 1.2) can go on simultaneously, simplify the operating procedure, improve water collection efficiency, and the mixed effect between the swimming pool water in each water cavity of adopting is better.
Optionally, the flabellum still includes the installation piece, go out appearance pipe one end protrusion the setting at the bottom of the chamber of hybrid chamber, the installation piece suit in go out the appearance pipe and be located the one end in the hybrid chamber, it is a plurality of the blade is fixed in the installation piece is outer along and along installation piece circumference interval sets up, it protrudes to go out appearance pipe tip the installation piece sets up, go out appearance pipe lateral wall and seted up a plurality of spacing mouths of a river along the circumference interval, install the spacing water pipe that restriction flabellum breaks away from and communicates out the appearance pipe in the spacing mouth of a river.
Through adopting above-mentioned technical scheme, the installation piece passes through spacing water pipe restriction on a kind pipe, and spacing water pipe can be followed the water sample entering play kind pipe after absorbing the stirring all around for the installation of flabellum does not influence the output of water sample, and the swimming pool water of input mixing chamber gets into the middle zone that a plurality of blades enclosed through the clearance between the blade in each water sampling chamber, carries out the vortex, and mixed effect further improves.
In summary, the present application includes at least one of the following beneficial technical effects:
1. the water samples of all sampling points are mixed, then the mixed swimming pool water is extracted as sample water, and then subsequent water sample detection is carried out, so that the number of the water samples is reduced, and meanwhile, data which can reflect the water condition of the swimming pool can be obtained accurately, and daily detection of the swimming pool water is facilitated;
2. The water sampling cavities are used for receiving swimming pool water collected by each collecting point in a one-to-one correspondence mode, the water sampling pistons are driven by the water sampling rods to move in the water sampling cavities, vacuum environments are formed in the water sampling cavities, then the water suction hoses absorb the swimming pool water from the collecting points and send the swimming pool water into the water sampling cavities, the mixing pistons are driven by the mixing rods to move upwards in the mixing cavities, vacuum environments are formed in the mixing cavities, and therefore the water in each water sampling cavity is sucked into the mixing cavities to be mixed;
3. the one end that the trace is located water sampling pole top is used for restricting on the one hand that water sampling pole breaks away from the water sampling chamber, be used for the mixing rod to drive the water sampling pole simultaneously again and move down, the mixing chamber is followed water sampling chamber extraction swimming pool water and is adopted the chamber to push into the swimming pool water and go on simultaneously to the mixing chamber, make the mixture of swimming pool water more simple and convenient, water sampling pole shifts up when extracting the swimming pool water from the swimming pool, can not receive the interference of trace, make things convenient for the collection of swimming pool water, can not receive the restriction of water sampling pole again when mixing rod moves down, make things convenient for extruding of appearance water.
Drawings
FIG. 1 is a schematic structural diagram 1 of a water collecting device;
FIG. 2 is a schematic structural diagram of a water collecting apparatus 2;
FIG. 3 is a partial cross-sectional view of a water production apparatus at a water production chamber;
fig. 4 is a schematic structural view of a water collecting device without a mixing piston and mixing rod device:
FIG. 5 is a schematic view of the installation structure of the sample outlet tube and the fan blade;
FIG. 6 is a schematic structural view of a mixing rod;
FIG. 7 is a schematic view of the mounting structure of the sample outlet hose, the water stop clip and the sterile water sampling bag.
Description of reference numerals: 1. water collecting shell; 11. a water collecting piston; 12. a water production rod; 121. connecting columns; 122. drawing the column; 13. a mixing piston; 131. a placement cavity; 14. a mixing rod; 141. a handle; 142. a cantilever plate; 15. a mixing hole; 16. a sample outlet pipe; 161. limiting a water gap; 162. a limiting water pipe; 21. a support bar; 22. a linkage rod; 3. a fan blade; 31. mounting a sheet; 32. a blade; 4. an aseptic water collecting bag; 41. a sterile sampling tube; 42. aseptic sample outlet pipes; 5. a water stop clip; 6. supporting legs; 7. braking the caster; 8. a waste water box; 81. a waste water port; 82. a plug; 91. a water suction hose; 92. a sample outlet rubber tube; 921. sampling and tube dividing; 93. a tie strap; 94. and (7) supporting the column.
Detailed Description
The present application is described in further detail below with reference to figures 1-6.
The embodiment of the application discloses a method for detecting microorganisms in swimming pool water. A method for detecting microorganisms in swimming pool water, which comprises the following steps:
1) collecting sample water through water collecting equipment;
referring to fig. 1 and 2, the water sampling equipment includes water sampling shell 1, water sampling piston 11, water sampling rod 12, mixing piston 13, mixing rod 14, the interlock subassembly, flabellum 3, aseptic water sampling bag 4 and stagnant water clamp 5, water sampling shell 1 upside opening and internal partitioning become a plurality of cavity including being located the biggest hybrid chamber in the middle and being located a plurality of water sampling chamber of hybrid chamber outer lane, water sampling shell 1 below is provided with supporting legs 6, the quantity of supporting legs 6 can be four, four sides of water sampling shell 1 are established respectively to four supporting legs 6, supporting legs 6 upper end and water sampling shell 1 welded fastening, a brake truckle 7 is fixed to every supporting legs 6 lower extreme, make water sampling equipment can convenient removal can settle in situ again.
Referring to fig. 1 and 2, a wastewater box 8 is arranged between four supporting legs 6, four sides of the wastewater box 8 are welded and fixed with the middle parts of the four supporting legs 6 in a one-to-one correspondence manner, a supporting column 94 is vertically and integrally arranged in the middle part of the wastewater box 8 and used for arranging the sterile water sampling bag 4, and a wastewater opening 81 and a plug 82 are formed in the wastewater box 8 and used for discharging received wastewater.
Referring to fig. 1, a plurality of water sampling pistons 11 are slidably mounted in a plurality of water sampling cavities in one-to-one correspondence, the water sampling pistons 11 are attached to the cavity walls of the water sampling cavities, so that when the water sampling pistons 11 slide in the water sampling cavities, a sealing state is maintained between the water sampling cavities and the water sampling pistons 11, a sealing rubber layer is sleeved and adhesively fixed outside the water sampling pistons 11, a plurality of water sampling rods 12 are integrally fixed on the water sampling pistons 11 in one-to-one correspondence, connecting columns 121 and drawing columns 122 are integrally arranged on the water sampling rods 12, the connecting columns 121 and the drawing columns 122 are coaxial with the water sampling rods 12, the diameters of the connecting columns 121 are smaller than those of the water sampling rods 12 and those of the drawing columns 122, and the drawing columns 122 conveniently draw the water sampling rods 12, so that the water sampling pistons 11 are driven to slide in the water sampling cavities.
Referring to fig. 3, the bottom of the water collecting cavity is provided with a water absorbing hose 91 communicated with the outside and a one-way valve, the one-way valve on the water absorbing hose 91 controls the water to enter the water collecting cavity but not flow out of the water collecting cavity, and the water in the swimming pool is absorbed from the swimming pool through the water absorbing hose 91.
Referring to fig. 1, mixing piston 13 slides and installs in the mixing chamber, and mixing piston 13 and the chamber wall laminating of mixing chamber for mixing piston 13 keeps a sealing state between mixing chamber and mixing piston 13 when sliding in the mixing chamber, mixing piston 13 is outer also the suit and the gluing is fixed with one deck sealing rubber layer, mixing rod 14 is integrative fixed with mixing piston 13, mixing rod 14 keeps away from mixing piston 13's one end and is provided with handle 141, conveniently stimulates mixing rod 14.
Referring to fig. 1, the linkage assemblies are provided with a plurality of water production rods 12 corresponding to the linkage assemblies one by one, each linkage assembly comprises a support rod 21 and a linkage rod 22, each linkage assembly comprises two support rods 21, the two support rods 21 are vertically fixed on the part between the mixing cavity and the corresponding water production cavity, and the middle part of each linkage rod 22 is positioned between the two support rods 21 and hinged between the two support rods 21.
Referring to fig. 1, the side wall of the mixing rod 14 is provided with a plurality of overhanging plates 142, one end of the linkage rod 22 is located on the upper side of the overhanging plates 142, and the overhanging plates 142 are arranged, so that the mixing rod 14 can better push the linkage rod 22, the diameter and the weight of the mixing rod 14 are not too large, interference between adjacent linkage rods 22 is not easy, the other end of the linkage rod 22 is located between the drawing column 122 and the water sampling column 12, and an arc notch is formed at one end of the linkage rod 22 located between the water sampling column 12 and the drawing column 122, so that interference between the connecting column 121 and the linkage rod 22 is avoided.
Referring to fig. 4, the mixing cavity is communicated with the water sampling cavities one by arranging mixing holes 15, a one-way valve is arranged in each mixing hole 15, and the one-way valve in each mixing hole 15 controls water to enter the mixing cavity from the water sampling cavity but not flow back to the water sampling cavity.
Referring to fig. 4 and 5, a sample outlet pipe 16 communicated with the outside is arranged in the middle of the bottom of the mixing chamber, and a one-way valve is installed on the sample outlet pipe 16, and the one-way valve on the sample outlet pipe 16 controls the sample water in the mixing chamber to flow out of the mixing chamber only through the sample outlet pipe 16 but not through the sample outlet pipe 16.
Referring to fig. 4 and 5, the fan blade 3 includes a mounting plate 31 and a plurality of blades 32, one end of the sample outlet pipe 16 protrudes out of the cavity bottom of the mixing cavity, the mounting plate 31 is sleeved at one end of the sample outlet pipe 16 located in the mixing cavity, the end of the sample outlet pipe 16 is higher than the mounting plate 31, the plurality of blades 32 are circumferentially spaced along the mounting plate 31, and a gap between adjacent blades 32 is used for sample water to pass through.
Referring to fig. 4 and 6, the side wall of the mixing piston 13 facing the bottom of the mixing cavity is provided with a placement cavity 131, so that when the mixing piston 13 abuts against the bottom of the mixing cavity, the fan blades 3 can enter the placement cavity 131, and the fan blades 3 do not hinder the mixing piston 13 from moving downwards.
Referring to fig. 4 and 5, the axes of the mixing holes 15 are arranged towards the blades 32 of the fan blades 3, and the acting force of the swimming pool water flowing into the mixing holes 15 on the blades 32 is spiral, so that the fan blades 3 are pushed to rotate towards the same direction, the cavity bottom of the mixing cavity and the cavity bottom of the water collecting cavity are partially arranged in the mixing holes 15, so that the mixing holes 15 are communicated with the cavity bottom of the mixing cavity, and the negative pressure environment generated when the mixing piston 13 moves can act on the one-way valve in the mixing holes 15 at the first time, thereby being beneficial to the inflow of the water sample.
Referring to fig. 4 and 5, a plurality of spacing nozzles 161 have been seted up along circumference interval to the play appearance pipe 16 lateral wall, install the spacing water pipe 162 that restriction flabellum 3 breaks away from and communicates out appearance pipe 16 in the spacing nozzle 161, and spacing water pipe 162 one end cartridge sets up the restriction flabellum 3 in spacing nozzle 161 other end overhang and breaks away from, and the upper portion of the one end of spacing water pipe 162 overhang is uncovered, is favorable to water along flowing into in the play appearance pipe 16.
Referring to fig. 2 and 7, a sample outlet rubber tube 92 is fixedly inserted into one end of the sample outlet tube 16 outside the mixing cavity, one end of the sample outlet rubber tube 92 is fixedly inserted into the sample outlet tube 16, the other end of the sample outlet rubber tube 92 is integrally arranged and divided into two sample outlet branch tubes 921, the sterile water sampling bag 4 is placed on a support column 94, the sterile water sampling bag 4 is provided with a sterile sample inlet tube 41 and a sterile sample outlet tube 42, the sterile sample inlet tube 41 and the sterile sample outlet tube 42 are respectively provided with a one-way valve, so that the sterile water sampling bag 4 can only input sample water through the sterile sample inlet tube 41 and discharge the sample water through the sterile sample outlet tube 42, the water stop clamp 5 is arranged on one sample outlet branch tube 921, the other sample outlet branch tube 921 is fixedly inserted into the sterile sample inlet tube 41 of the sterile water sampling bag 4, the water stop clamp 5 and the sterile water sampling bag 4 are directly fixed through a lacing 93, and one of the water stop clamp 5 and the sterile water sampling bag 4 are fixed with the sample outlet branch tubes 921, so that the sterile water-collecting bag 4 is not easy to fall into the waste water box 8.
1.1) collecting swimming pool water of each sampling point;
investigating the distribution condition of personnel in each area of the swimming pool, dividing collection points according to the personnel in the area, arranging more collection points at the intensive personnel position, collecting water samples from the middle layer to the surface layer at each collection point, pulling a water collection rod 12 by holding a water suction hose 91 during collection, putting down the water suction hose 91 to move from the surface layer to the middle layer of the collection point, completing the collection of water in the swimming pool in the process that the water suction hose 91 moves to the middle layer, collecting the water in the swimming pool at each collection point by the same method, and collecting one or more collection points in each water collection cavity;
1.2) mixing the collected swimming pool water of each sampling point;
the mixing rod 14 is driven by the handle 141, the mixing rod 14 drives the mixing piston 13, negative pressure is formed in the mixing cavity, and therefore the swimming pool water in the water collecting cavity is sucked into the mixing cavity to be mixed;
1.3) extracting sample water from the mixed swimming pool water;
the swimming pool water in the mixing cavity is discharged through the sample discharging rubber tube 92 by moving the mixing piston 13 downwards, one sample discharging branch tube 921 is closed through the water stopping clamp 5, then part of sample water is extruded through the other sample discharging branch tube 921, the sterile water sampling bag 4 is fixedly inserted into the other sample discharging branch tube 921, sample water is injected, then the water stopping clamp 5 is taken down, and the rest sample water is discharged from the other sample discharging branch tube 921;
2) Preparing a reserve culture medium;
adding 3.5g of dipotassium phosphate, 5g of yeast extract, 5g of beef extract and 10g of peptone into a beaker containing 900mL of distilled water, adjusting the pH value to 7.27.4 after dissolving, adding 10-20 g of agar, heating for dissolving, supplementing to 1000mL of distilled water, filtering with absorbent cotton or lint while hot, adding 10g of lactose, quantitatively subpackaging in a flask after uniformly mixing, autoclaving at 115 ℃ for 20min, and cooling in a dark place for later use;
3) preparing a plate culture medium;
heating and melting the culture medium in the step 2), and mixing the basic fuchsin ethanol solution with the culture medium according to the ratio of 1: 50, sucking a certain amount of basic fuchsin solution by using a sterilization pipette and placing the basic fuchsin solution in a sterilized empty test tube; then according to the proportion of 1: 200, putting the required anhydrous sodium sulfite into another sterilized empty test tube, adding a little sterilized water to dissolve the anhydrous sodium sulfite, and boiling and sterilizing the anhydrous sodium sulfite in a boiling water bath for 10 min. Sucking the sterilized sodium sulfite solution by using a sterilization suction pipe, and dropwise adding the sterilized sodium sulfite solution into the alkaline fuchsin ethanol solution until the dark red fades to light pink; adding the mixed solution of sodium sulfite and basic fuchsin into a melted reserve culture medium, fully and uniformly mixing, immediately pouring a proper amount of the culture medium into a sterilized empty plate, and cooling and solidifying the culture medium and then placing the culture medium into a refrigerator for later use; the culture is not suitable for more than two weeks based on the preservation in a refrigerator, and the culture medium cannot be reused if the culture medium is changed from light red to deep red;
4) Preparing lactose peptone culture solution;
5) and (3) sterilizing the filter membrane: the filter membrane was placed in a beaker containing distilled water, and sterilized by boiling three times, each for 15 min. After the first two times of boiling, water washing needs to be replaced for 2-3 times to remove residual solvent;
6) and sterilizing a filter: sterilizing at 121 deg.C under high pressure for 20min or sterilizing with ignited alcohol cotton ball flame;
7) and water sample filtration: clamping the edge part of the sterilization filter membrane by using a sterile forceps, and sticking the sterilization filter membrane on a filter bed with the rough surface facing upwards; fixing the filter, injecting 100mL water sample such as water sample with more bacteria and capable of reducing water filtration sample amount or diluting the water sample) into the filter, opening the filter door, and performing suction filtration under 0.5 × 10Pa (-0.5 atmospheric pressure);
8) and culturing, namely pumping the water sample for about 5s after the water sample is filtered, closing a valve of the filter, and taking down the filter. Clamping the edge part of the filter membrane by using a sterilization forceps, transferring the filter membrane onto a lactose agar separation culture medium, wherein the interception surface of the filter membrane is upward, the filter membrane is completely attached to the culture medium, and no air bubble is left between the filter membrane and the culture medium; then inverting the plate, and putting the plate into a thermostat at 36 +/-1 ℃ for culturing for 18-24 hours;
9) picking the bacterial colony which accords with the gram stain and microscopic examination:
reddish-purple, colonies with metallic luster;
Dark red, colonies with no or slight metallic luster;
light red, the center color is darker colony;
10) inoculating bacillus subtilis which is gram-negative in staining into lactose peptone culture solution, and culturing for 48 hours in an environment of 36 +/-1 ℃, wherein a person producing acid and gas proves to be positive in coliform group;
11) the number of colonies growing on the filters, confirmed as coliform groups, was calculated:
calculating the colony number of coliform bacteria which is proved to grow on the filter membrane, and multiplying by 10 to obtain the coliform bacteria number in each 1000mL water sample.
The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: equivalent changes in structure, shape and principle of the present application shall be covered by the protection scope of the present application.
Claims (7)
1. A method for detecting microorganisms in swimming pool water, which comprises the following steps:
1) collecting sample water through water collecting equipment;
1.1) collecting the water of the swimming pool at each sampling point, wherein each collecting point collects the water sample from the middle layer to the surface layer, the distribution condition of personnel in each area of the swimming pool is investigated before the collecting points are determined, the collecting points are divided according to the personnel in the area, and more collecting points are arranged at the personnel dense positions;
1.2) mixing the collected swimming pool water at each sampling point;
1.3) extracting sample water from the mixed swimming pool water;
2) preparing a reserve culture medium;
3) preparing a plate culture medium;
4) preparing lactose peptone culture solution;
5) sterilizing the filter membrane;
6) sterilizing a filter;
7) filtering the water sample;
8) and culturing;
9) picking the bacterial colony to perform gram staining and microscopic examination;
10) inoculating gram-negative bacillus-free bacillus into lactose peptone culture solution, and culturing for 48h in an environment with the temperature of 36 +/-1 ℃, wherein a person producing acid and gas is proved to be positive in coliform;
11) calculating the colony number of coliform bacteria which are proved to grow on the filter membrane;
the water sampling device in the step 1) comprises a water sampling shell (1), a water sampling piston (11), a water sampling rod (12), a mixing piston (13) and a mixing rod (14), a mixing cavity and a plurality of water sampling cavities positioned on the outer ring of the mixing cavity are arranged in the water sampling shell (1), the water sampling pistons (11) are provided with a plurality of pistons which are arranged in the water sampling cavity in a sliding way in one-to-one correspondence, a plurality of water production rods (12) are arranged, the water production rods (12) are correspondingly fixed on the water production pistons (11) one by one, the mixing piston (13) is arranged in the mixing cavity in a sliding way and fixes the mixing rod (14), mixing holes (15) are arranged between the mixing cavity and the water production cavities and are communicated with each other and provided with one-way valves, the bottom of the water sampling cavity is provided with a water absorbing hose (91) communicated with the outside, and the bottom of the mixing cavity is provided with a sample outlet pipe (16) communicated with the outside and provided with a one-way valve.
2. The method as claimed in claim 1, wherein the method further comprises the steps of: the water production device is characterized in that a plurality of linkage assemblies are arranged on the water production shell (1), the linkage assemblies correspond to the water production rods (12) one by one, each linkage assembly comprises a supporting rod (21) and a linkage rod (22), the supporting rods (21) are vertically fixed on the water production shell (1), the middle parts of the linkage rods (22) are rotatably installed at one ends, far away from the water production shell (1), of the supporting rods (21), and one ends of the linkage rods (22) are located at the positions above the water production rods (12) and the other ends of the linkage rods (22) are located above the mixing rod (14).
3. The method as claimed in claim 2, wherein the method further comprises the steps of: mixing rod (14) lateral wall is provided with overhang board (142), trace (22) one end is located overhang board (142) upside, be provided with spliced pole (121) on adopting water pole (12) and draw post (122), spliced pole (121) and draw post (122) all with adopt water pole (12) coaxial, just the diameter of spliced pole (121) is less than adopt the diameter of water pole (12) and draw the diameter of post (122), trace (22) other end is located draw post (122) with adopt between water pole (12).
4. The method as claimed in claim 3, wherein the method further comprises the steps of: install out appearance rubber tube (92) on going out appearance pipe (16), it divides pipe (921) to be provided with two play appearances on the rubber tube (92) of going out the appearance, water sampling equipment still includes aseptic water sampling bag (4) and stagnant water clamp (5), stagnant water clamp (5) set up in one go out the appearance and divide on pipe (921), another go out the appearance and divide pipe (921) with aseptic appearance pipe (41) cartridge of advancing of aseptic water sampling bag (4) is fixed, stagnant water clamp (5) are fixed with aseptic water sampling bag (4).
5. The method as claimed in claim 4, wherein the method further comprises the steps of: the sterile water sampling bag is characterized in that a plurality of supporting legs (6) are arranged below the water sampling shell (1), brake trundles (7) are arranged below the supporting legs (6), a waste water box (8) is arranged between the supporting legs (6), and a supporting column (94) used for placing the sterile water sampling bag (4) is arranged in the middle of the waste water box (8).
6. The method as claimed in claim 5, wherein the detection of the microorganisms in the swimming pool water comprises: water collecting equipment still includes flabellum (3), flabellum (3) include a plurality of blades (32), flabellum (3) rotate install in at the bottom of the chamber of hybrid chamber, mixing piston (13) orientation settle chamber (131) that supply flabellum (3) to get into are seted up to the lateral wall at the bottom of the hybrid chamber, and are a plurality of the axis orientation of mixing hole (15) blade (32) of flabellum (3) set up.
7. The method as claimed in claim 6, wherein the method further comprises the steps of: fan blade (3) are still including installation piece (31), go out appearance pipe (16) one end protrusion the setting at the bottom of the chamber of hybrid chamber, installation piece (31) suit in go out appearance pipe (16) and be located one end in the hybrid chamber, it is a plurality of blade (32) are fixed in installation piece (31) outer edge and follow installation piece (31) circumference interval sets up, go out appearance pipe (16) tip protrusion installation piece (31) set up, a plurality of spacing mouths of a river (161) have been seted up along the circumference interval to play appearance pipe (16) lateral wall, install spacing water pipe (162) that restriction flabellum (3) break away from and feed through out appearance pipe (16) in spacing mouth of a river (161).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110167455.2A CN112760357B (en) | 2021-02-05 | 2021-02-05 | Method for detecting microorganisms in swimming pool water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110167455.2A CN112760357B (en) | 2021-02-05 | 2021-02-05 | Method for detecting microorganisms in swimming pool water |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112760357A CN112760357A (en) | 2021-05-07 |
| CN112760357B true CN112760357B (en) | 2022-06-28 |
Family
ID=75705257
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110167455.2A Active CN112760357B (en) | 2021-02-05 | 2021-02-05 | Method for detecting microorganisms in swimming pool water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112760357B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103234779A (en) * | 2013-05-15 | 2013-08-07 | 中农宸熙（福建）物联科技有限公司 | Multi-point-position water quality sampling and detecting process and specific device thereof |
| CN104789637A (en) * | 2015-04-01 | 2015-07-22 | 郭会清 | Method for quickly detecting coliform groups in textile |
| CN110844096A (en) * | 2019-12-16 | 2020-02-28 | 杜志刚 | Water sampling environmental protection unmanned aerial vehicle |
| CN111366414A (en) * | 2018-12-26 | 2020-07-03 | 王望珩 | Novel water sample collection device for multi-point environment detection |
| CN212134677U (en) * | 2020-05-29 | 2020-12-11 | 江苏财经职业技术学院 | Floated water quality monitoring device based on thing networking |
-
2021
- 2021-02-05 CN CN202110167455.2A patent/CN112760357B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103234779A (en) * | 2013-05-15 | 2013-08-07 | 中农宸熙（福建）物联科技有限公司 | Multi-point-position water quality sampling and detecting process and specific device thereof |
| CN104789637A (en) * | 2015-04-01 | 2015-07-22 | 郭会清 | Method for quickly detecting coliform groups in textile |
| CN111366414A (en) * | 2018-12-26 | 2020-07-03 | 王望珩 | Novel water sample collection device for multi-point environment detection |
| CN110844096A (en) * | 2019-12-16 | 2020-02-28 | 杜志刚 | Water sampling environmental protection unmanned aerial vehicle |
| CN212134677U (en) * | 2020-05-29 | 2020-12-11 | 江苏财经职业技术学院 | Floated water quality monitoring device based on thing networking |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112760357A (en) | 2021-05-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110628603A (en) | A incubator for microbial inoculum | |
| CN101899391B (en) | Special spectrum airlift photobioreactor | |
| CN102827766B (en) | Photobioreactor for large-scale production of astaxanthin by haematococcus pluvialis | |
| CN201756550U (en) | Fully-sealed bacteria collection ampoule incubator | |
| CN112760357B (en) | Method for detecting microorganisms in swimming pool water | |
| CN110804644A (en) | Method and device for detecting multiple microorganisms in food | |
| CN201132831Y (en) | Circle releasing device for carbonic acid gas by pool-cultured spirulina | |
| CN116622637A (en) | Stem cell culture method for treating high paraplegia | |
| CN215517424U (en) | Algae cultivation system with growth monitoring system | |
| CN113951202B (en) | Leiocassis longissimus seedling artificial containers | |
| CN106701547A (en) | Device for collecting gas volatile matters produced by biological metabolism and real-time collection method thereof | |
| CN102128736B (en) | Dry land nitrification and denitrification field in-situ measuring device and method for testing by using same | |
| CN115445274B (en) | Device and method for separating and collecting zooplankton metabolism emission organic carbon | |
| CN107828640B (en) | Microorganism collector in water | |
| CN106755101A (en) | A kind of is the method for iPS cells by the reprogramming of equinovarus Urine in Patients cast-off cells | |
| CN206638480U (en) | A kind of filling drinking water quickly collects fungus strain system | |
| CN114032161B (en) | Algae cultivation system | |
| CN114410460A (en) | Microbial cultivation device for sewage treatment | |
| CN102002482A (en) | Method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses | |
| CN215065493U (en) | Water sample collector for continuously extracting microcystin samples in freshwater environment | |
| CN103013818A (en) | Bacterium collecting incubator capable of realizing positive-pressure and negative-pressure filtering | |
| CN202744558U (en) | Photobioreactor for using haematococcus pluvialis to produce astaxanthin on large scale | |
| CN218507764U (en) | Fermentation system and thallus enrichment purification device | |
| CN201886001U (en) | Field in-situ determining device for nitrification and denitrification of dry land | |
| CN206843509U (en) | A kind of novel cell culture device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |