CN112760169A - Acer truncatum seed essential oil, and preparation method and application thereof - Google Patents
Acer truncatum seed essential oil, and preparation method and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
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- Bioinformatics & Cheminformatics (AREA)
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Abstract
The invention discloses acer truncatum seed essential oil, a preparation method and application thereof, and belongs to the technical field of natural active ingredient extraction. The preparation method of the Acer truncatum seed essential oil comprises the following steps: step 1: taking Acer truncatum seeds, freeze-drying, crushing and sieving to obtain Acer truncatum seed coarse powder; step 2: taking the Acer truncatum seed coarse powder obtained in the step 1, and performing supercritical CO treatment2Extracting, purifying by stage with separation kettle I and separation kettle II, collecting separation kettle IIThe essential oil in the product is the Acer truncatum seed essential oil. The invention also discloses the acer truncatum seed essential oil prepared by the preparation method and application thereof. The preparation method of the Acer truncatum seed essential oil has high essential oil yield of 4.9-5.24%; the essential oil has good purity, wherein the relative content of an active ingredient alpha-bisabolol is 71.6-81%; the operation condition is mild and controllable.
Description
Technical Field
The invention relates to Acer truncatum seed essential oil, a preparation method and application thereof, and belongs to the technical field of natural active ingredient extraction.
Background
The plant essential oil is an aromatic substance extracted from plants, has physiological activities of resisting bacteria and corrosion, improving immunity, resisting virus attack, cleaning skin, controlling oil content, removing under-eye dark circles, promoting regeneration of damaged tissues and the like, and is a plant functional extract with wide application market.
Acer acutifolia, entitled Callicarpa Longissima (Hemsl.) Merr, is a plant of Verbenaceae family, is the hand-sticking wind of seventy-two wind in Guangxi characteristic Yao medicine Hu niu Zhang Feng, also named as snow-prominence, red-drug seed, rising and falling in the sun, and wearing bone maple (Guangxi) and ox tongue (Fujian). The medicine has warm nature, enters liver and lung meridians, has pungent and slightly bitter taste, and is often used for treating poliomyelitis sequela, traumatic injury, abdominal pain due to cold accumulation, rheumatism, arthralgia, undefined lump, puerperal rheumatism, venomous snake bite, etc.
However, at present, no report exists on the efficient preparation of essential oil from Acer truncatum seeds. In view of this, there is a need to provide a method for preparing an essential oil from Acer truncatum seeds, so as to solve the deficiencies of the prior art.
Disclosure of Invention
The invention aims to provide a preparation method of Acer truncatum seed essential oil. The preparation method of the Acer truncatum seed essential oil has high essential oil yield of 4.9-5.24%; the essential oil has good purity, wherein the relative content of an active ingredient alpha-bisabolol is 71.6-81%; the operation condition is mild and controllable.
The technical scheme for solving the technical problems is as follows: a preparation method of Acer truncatum seed essential oil comprises the following steps:
step 1: taking Acer truncatum seeds, freeze-drying, crushing and sieving to obtain Acer truncatum seed coarse powder;
step 2: taking the Acer truncatum seed coarse powder obtained in the step 1, and performing supercritical CO treatment2Extracting, carrying out fractional purification by a separation kettle I and a separation kettle II, and collecting essential oil in the separation kettle II, namely the acer truncatum seed essential oil.
The principle of the preparation method of the Acer truncatum seed essential oil is as follows:
in the step 1 of the invention, the Acer truncatum seeds are freeze-dried, so that the loss of heat-sensitive, easily-decomposed and easily-oxidized components in volatile oil components caused by a conventional heating and drying mode can be avoided.
In step 2 of the invention, supercritical CO is adopted for the Acer truncatum seed coarse powder2The extraction has high yield of the essential oil, and the yield is 4.9 to 5.24 percent; the essential oil has good purity, wherein the relative content of an active ingredient alpha-bisabolol is 71.6-81%; the operation condition is mild and controllable.
The preparation method of the Acer truncatum seed essential oil has the beneficial effects that:
1. the preparation method of the Acer truncatum seed essential oil has high essential oil yield of 4.9-5.24%; the essential oil has good purity, wherein the relative content of an active ingredient alpha-bisabolol is 71.6-81%; the operation condition is mild and controllable.
2. The preparation method of the Acer truncatum seed essential oil is simple to operate, low in cost, wide in market prospect and suitable for large-scale popularization and application.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, in the step 1, the temperature of the freeze drying is between 55 ℃ below zero and 60 ℃ below zero, and the time is between 24 and 48 hours.
The adoption of the further beneficial effects is as follows: by adopting the parameters, the freeze drying effect is better.
Further, in step 1, the mesh size of the sieve is 40 meshes.
The adoption of the further beneficial effects is as follows:by adopting the parameters, the obtained Acer truncatum seed coarse powder is more beneficial to subsequent supercritical CO2And (4) extracting.
Further, in the step 1, the water content of the Acer truncatum seed coarse powder is 4-6 wt%.
The adoption of the further beneficial effects is as follows: controlling the water content of the Acer truncatum seed coarse powder, destroying the continuity of water films between cells, increasing the contact between an extraction solvent and essential oil, and improving the dissolution of the essential oil. Performing supercritical CO with the seeds with the water content and fresh seeds2Compared with extraction, the yield of the essential oil of the Acer truncatum seeds is improved by more than 1.8 percent.
Further, in step 2, the supercritical CO is2The specific parameters extracted are: by supercritical CO2Is solvent, ethanol with 95-100 vol% concentration is entrainer, the flow ratio of entrainer and solvent is 10-15%, and CO2The flow is 20L/h-30L/h, the extraction pressure is 12MPa-14MPa, the extraction temperature is 35-37 ℃, and the extraction time is 1h-2 h; the pressure of the separation kettle I is 8MPa-10MPa, and the temperature is 35-37 ℃; the pressure of the separation kettle II is 5MPa-7MPa, and the temperature is 31 ℃ to 33 ℃.
The further beneficial effects are as follows: by adopting the parameters, the invention can improve the yield of the essential oil and the relative content of the functional component alpha-bisabolol in the essential oil, and compared with the conventional steam extraction, the yield of the essential oil and the relative content of the alpha-bisabolol are respectively improved by 1.58 percent and 22.2 percent. Wherein the supercritical CO can be modified by adding an entrainer2The solubility range of the product is widened, and the extraction capacity is improved. alpha-Bisabolol (Bisabolol), also known as Bisabolol and ledebouriella seselol, has antibacterial and anti-inflammatory effects, can protect and cure skin, and can accelerate the healing process of skin. Therefore, the relative content of the alpha-bisabolol in the acer truncatum seed essential oil is high, and the subsequent application is facilitated.
The second object of the invention is to provide the Acer truncatum seed essential oil prepared by the preparation method of the Acer truncatum seed essential oil.
The technical scheme for solving the technical problems is as follows: the Acer truncatum seed essential oil prepared by the preparation method of the Acer truncatum seed essential oil.
The Acer truncatum seed essential oil has the beneficial effects that:
the acer truncatum seed essential oil disclosed by the invention is high in relative content of alpha-bisabolol, good in antibacterial, anti-inflammatory and whitening effects and more beneficial to subsequent application.
The third purpose of the invention is to provide the application of the acer truncatum seed essential oil prepared by the preparation method of the acer truncatum seed essential oil. The prepared Acer acutus seed essential oil can be used for preparing antibacterial and anti-inflammatory medicines, has obvious anti-inflammatory and whitening effects, and can be used for treating and nursing inflammatory skin such as skin folliculitis, cellulitis and acne.
The technical scheme for solving the technical problems is as follows: the Acer truncatum seed essential oil prepared by the preparation method of the Acer truncatum seed essential oil is applied to preparing antibacterial and anti-inflammatory medicines.
The inventor of the application carries out pharmacological research and finds that the prepared Acer truncatum essential oil can obviously inhibit mouse macrophage RAW264.7 cells from secreting NO and IC under the safe concentration of cells50Has a value of 52.4 μ g/ml, and has tyrosinase activity inhibiting ability, IC50The value is 152.4 mug/ml, and the acer truncatum seed essential oil has no toxic or side effect on skin, and shows that the acer truncatum seed essential oil can be applied to preparing antibacterial and anti-inflammatory medicines.
The application of the acer truncatum seed essential oil has the beneficial effects that:
the prepared Acer acutus seed essential oil can be used for preparing antibacterial and anti-inflammatory medicines, has obvious anti-inflammatory and whitening effects, and can be used for treating and nursing inflammatory skin such as skin folliculitis, cellulitis and acne.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, the antibacterial and anti-inflammatory medicine comprises Acer acutangum essential oil and a pharmaceutically acceptable carrier.
The adoption of the further beneficial effects is as follows: the antibacterial and anti-inflammatory medicine can be used for treating and nursing inflammatory skin such as skin folliculitis, cellulitis and acne, and has obvious effect and no toxic or side effect.
Further, the pharmaceutically acceptable carrier is any one or more of a diluent, an absorption enhancer, an excipient, a surfactant, an adhesive, a wetting agent, a lubricant and a flavoring agent.
The adoption of the further beneficial effects is as follows: by adopting the carrier, more products with different dosage forms can be obtained, and the application range is enlarged.
Furthermore, the dosage form of the antibacterial and anti-inflammatory drug is any one of a solid preparation, a semi-solid preparation and a liquid preparation.
The adoption of the further beneficial effects is as follows: the Acer truncatum essential oil has stable physical and chemical properties, can be prepared into various formulations, and is convenient for different people to use.
Drawings
FIG. 1 is a total ion chromatogram of Acer truncatum seed essential oil prepared in example 1 of the present invention.
FIG. 2 is a total ion chromatogram of Acer truncatum seed essential oil prepared in example 2 of the present invention.
Fig. 3 is a total ion chromatogram of the acer truncatum seed essential oil prepared in example 3 of the invention.
FIG. 4 is a total ion chromatogram of Acer truncatum seed essential oil prepared in comparative example 1 of the present invention.
FIG. 5 is a total ion chromatogram of Acer truncatum seed essential oil prepared in comparative example 2 of the present invention.
Detailed Description
The principles and features of this invention are described below in conjunction with the following detailed drawings, which are given by way of illustration only and are not intended to limit the scope of the invention.
Example 1
The preparation method of the acer truncatum seed essential oil comprises the following steps:
step 1: 500g of Acer acutangum seeds are taken and placed in a freeze dryer to be dried for 48 hours at minus 60 ℃ under reduced pressure, and the crushed Acer acutangum seeds are sieved by a 40-mesh sieve to obtain 205g of Acer acutangum seed coarse powder. The water content of the Acer truncatum seed coarse powder is 5.0 wt% as determined by a moisture analyzer.
Step 2: taking 205g of Acer truncatum seed coarse powder obtained in the step 1, and performing supercritical CO2Extracting, wherein the specific parameters are as follows: by supercritical CO2As solvent, 100% volume concentration ethanol as entrainer, entrainer and solvent flow ratio of 15%, CO2The flow rate is 30L/h, the extraction pressure is 13MPa, the extraction temperature is 36 ℃, and the extraction time is 2.0 h.
Carrying out fractional purification by a separation kettle I and a separation kettle II, wherein the pressure of the separation kettle I is 9MPa, and the temperature is 36 ℃; the pressure of the separation kettle II is 6MPa, and the temperature is 32 ℃.
And (3) collecting 26.2g of essential oil in the separation kettle II to obtain the acer truncatum seed essential oil.
The yield of the acer truncatum seed essential oil of the example is calculated as follows:
experimental example 1: GC-MS analysis of Acer truncatum seed essential oil
GC-MS conditions:
(1) gas Chromatography (GC) conditions: the flow rate is 1ml/min under the constant-current mode; the sample introduction mode is GC automatic sample introduction, the sample introduction amount is 3 mu L, and the split ratio is set to be 10: 1; setting the injection port temperature of 250 ℃; carrier gas: nitrogen (volume fraction 99.999%); the temperature programming is as follows: the column temperature is initially 50 ℃, the temperature is raised to 150 ℃ at a rate of 10 ℃/min, the temperature is raised to 180 ℃ at a rate of 1 ℃/min, and the temperature is raised to 250 ℃ at a rate of 10 ℃/min.
(2) Mass spectrum conditions: electron Impact (EI); ionization energy is 70 eV; the ion source temperature is 230 ℃; a solvent delay time of 4 min; the temperature of the MS quadrupole rods is 150 ℃; the mass spectrum search library is a NIST standard general library.
The total ion chromatogram of the acer truncatum seed essential oil prepared in example 1 is shown in fig. 1.
And (3) detection results:
as shown in table 1, the acer truncatum essential oil obtained by fractional purification has simple component composition and the relative content of the active ingredient alpha-bisabolol is 81%.
TABLE 1 analysis of the components and relative contents of the essential oil of Acer truncatum seeds
Experimental example 2: evaluation of inhibition of tyrosinase activity by Acer truncatum seed essential oil
The inhibition effect of the Acer acutangula seed essential oil on the tyrosinase activity is determined by adopting a T/SHRH 015-2018 cosmetic-tyrosinase activity inhibition experiment method.
Weighing a proper amount of acer truncatum seed essential oil and positive control kojic acid, dissolving the acer truncatum seed essential oil and the positive control kojic acid by using a small amount of DMSO (dimethyl sulfoxide), diluting the acer truncatum seed essential oil and the positive control kojic acid into sample solutions with serial concentrations by using a disodium hydrogen phosphate-citric acid buffer solution, and detecting and calculating according to the experimental steps of T/SHRH 015-2018 cosmetic-tyrosinase activity inhibition experimental method.
The results are shown in Table 2.
TABLE 2 inhibition of tyrosinase activity by Acer truncatum seed essential oil
| Sample to be tested | IC50(μg/ml) |
| Acer truncatum seed essential oil | 152.4 |
| Kojic acid | 68.3 |
As can be seen from Table 2, IC of kojic acid50Is 68.3 mugml, within the range specified by the method (0.05mg/ml-0.15mg/ml), indicates that the test system is effective. Compared with the positive, the acer truncatum seed essential oil shows a relatively high inhibition effect on tyrosinase. The experimental result shows that the acer truncatum seed essential oil prepared in the embodiment 1 of the invention has an application prospect in development and utilization of whitening products.
Experimental example 3: evaluation of anti-inflammatory Activity of Acer truncatum seed essential oil
In the experiment, the content of NO is measured by adopting a Griess method, and the inhibition effect of the Acer truncatum seed essential oil on the generation of NO by LPS-induced mouse macrophage RAW264.7 is evaluated. The result shows that the acer truncatum seed essential oil can effectively inhibit NO generation and inhibit IC5052.4. mu.g/ml, positive control parthenolide IC50It was 0.45. mu.M. Experimental results show that the acer truncatum seed essential oil prepared in the embodiment 1 of the invention has good anti-inflammatory activity.
Experimental example 4: acute toxicity test of Acer truncatum seed essential oil transdermal drug delivery
In this experiment, the safety of the acer truncatum seed essential oil for skin administration was evaluated according to the acute transdermal toxicity test method in 2007 edition of technical Specification for cosmetic safety-2015.
Step 1: grouping animals
After 20 SPF SD rats with the body weight of 200g-300g are taken and fed adaptively for 1 week, the rats are divided into a control group and a dosing group, wherein each group comprises 10 rats and each rat comprises a male rat and a female rat.
Step 2: molding and administration
Shearing hairs of the back of each rat with an area of 6cm x 6cm, smearing Acer truncatum seed essential oil on the hair removing area of the administration group, smearing 2.2g/kg body weight of the medicine on the hair removing area of the control group, and smearing the same amount of pure water on the hair removing area of the control group. The smearing area is fixed with non-irritating adhesive tape to prevent animal sweet food. The administration period is given by conventional feed, and drinking water is not limited. Animals were observed for mortality and growth within 2 weeks of dosing.
And step 3: results
During experimental observation, rats grow normally at a dose of 2.2g/kg, and have no toxic phenomenon and no death within 2 weeks as shown in table 3, and the acer truncatum seed essential oil belongs to a non-toxic grade according to the skin toxicity grade of technical Specification for cosmetic safety-2015.
TABLE 3 acute percutaneous toxicity test results of Acer truncatum seed essential oil on rats
According to the pharmacological activity evaluation, the acer truncatum seed essential oil prepared in the embodiment 1 of the invention shows good anti-inflammatory and whitening effects, and a percutaneous acute toxicity test also shows that the acer truncatum seed essential oil belongs to a nontoxic grade, so that the acer truncatum seed essential oil prepared in the embodiment 1 of the invention can be used for preparing antibacterial and anti-inflammatory medicines.
The antibacterial and anti-inflammatory medicine comprises Acer acutangum essential oil and a pharmaceutically acceptable carrier.
The pharmaceutically acceptable carrier is any one or more of diluent, absorption enhancer, excipient, surfactant, adhesive, humectant, lubricant and correctant.
The dosage form of the antibacterial and anti-inflammatory drug is any one of a solid preparation, a semi-solid preparation and a liquid preparation.
Example 2
The preparation method of the acer truncatum seed essential oil comprises the following steps:
step 1: 500g of Acer acutangum seeds are taken and placed in a freeze dryer to be dried for 24 hours at the temperature of minus 55 ℃ under reduced pressure, and then the powder is crushed and sieved by a 40-mesh sieve, so that 209g of Acer acutangum seed coarse powder is obtained. The water content of the Acer truncatum seed coarse powder is 5.8 wt% as determined by a moisture analyzer.
Step 2: taking 209g of the Acer truncatum seed coarse powder obtained in the step 1, and performing supercritical CO2Extracting, wherein the specific parameters are as follows: by supercritical CO2As solvent, ethanol with 95% volume concentration as entrainer, the flow ratio of entrainer and solvent is 10%, and CO2The flow rate is 20L/h, the extraction pressure is 12MPa, the extraction temperature is 35 ℃, and the extraction time is 1 h.
Carrying out stage purification by a separation kettle I and a separation kettle II, wherein the pressure of the separation kettle I is 10MPa, and the temperature is 37 ℃; the pressure of the separation kettle II is 7MPa, and the temperature is 33 ℃.
And collecting 24.5g of essential oil in the separation kettle II to obtain the acer truncatum seed essential oil.
The yield of the acer truncatum seed essential oil of the example is calculated as follows:
the GC-MS analysis of the acer truncatum seed essential oil as in example 1 showed a relative alpha-bisabolol content of 76%.
The total ion chromatogram of the acer truncatum seed essential oil prepared in example 2 is shown in fig. 2.
Example 3
The preparation method of the acer truncatum seed essential oil comprises the following steps:
step 1: 500g of Acer acutangum seeds are taken and placed in a freeze dryer to be dried for 36 hours at minus 57 ℃ under reduced pressure, and the crushed materials are sieved by a 40-mesh sieve to obtain 210g of Acer acutangum seed coarse powder. The water content of the Acer truncatum seed coarse powder is 6.0 wt% as determined by a moisture analyzer.
Step 2: taking 210g of the Acer truncatum seed coarse powder obtained in the step 1, and performing supercritical CO2Extracting, wherein the specific parameters are as follows: by supercritical CO2As solvent, ethanol with the volume concentration of 98 percent is used as entrainer, the flow ratio of the entrainer to the solvent is 13 percent, and CO2The flow rate is 25L/h, the extraction pressure is 14MPa, the extraction temperature is 37 ℃, and the extraction time is 1.5 h.
Carrying out stage purification by a separation kettle I and a separation kettle II, wherein the pressure of the separation kettle I is 8MPa, and the temperature is 35 ℃; the pressure of the separation kettle II is 5MPa, and the temperature is 31 ℃.
And collecting 25.1g of essential oil in the separation kettle II to obtain the acer truncatum seed essential oil.
The yield of the acer truncatum seed essential oil of the example is calculated as follows:
the GC-MS analysis of the acer truncatum seed essential oil as in example 1 showed a relative alpha-bisabolol content of 71.6%.
The total ion chromatogram of the Acer truncatum seed essential oil prepared in example 3 is shown in FIG. 3.
Comparative example 1
Comparative example 1 differs from example 1 in that in step 1, Acer truncatum seeds were fresh and had a water content of 64.3 wt%, and were directly subjected to supercritical CO of step 2 without treatment2Extracting, and the rest is the same.
17.2g of Acer truncatum seed essential oil was prepared in comparative example 1.
Comparative example 1 the yield of acer truncatum seed essential oil was calculated as follows:
GC-MS analysis of the acer truncatum seed essential oil prepared in comparative example 1 showed that the relative content of alpha-bisabolol was 62.7% as in example 1.
The total ion chromatogram of the acer truncatum seed essential oil prepared in comparative example 1 is shown in fig. 4.
Compared with the comparative example 1, the yield of the acer truncatum seed essential oil can be improved by 1.8%, and the relative content of alpha-bisabolol can be improved by 18.3%.
Therefore, the water content of the acer truncatum seed coarse powder is controlled, the continuity of water films between cells is damaged, the contact between an extraction solvent and essential oil is increased, and the dissolution of the essential oil can be improved. Performing supercritical CO with the seeds with the water content and fresh seeds2Compared with extraction, the yield of the essential oil of the Acer truncatum seeds is improved by more than 1.8 percent.
Comparative example 2
Comparative example 2 differs from example 1 in that in step 2, supercritical CO was not used2Extracted according to the pharmacopoeia of the people's republic of China 2020 editionThe volatile oil extraction method is a conventional steam extraction method, and the rest steps are the same.
Comparative example 2 prepared 18.3g of Acer truncatum seed essential oil.
Comparative example 2 the yield of acer truncatum seed essential oil was calculated as follows:
GC-MS analysis of the acer truncatum seed essential oil prepared in comparative example 2 showed that the relative content of alpha-bisabolol was 58.8% as in example 1.
The total ion chromatogram of the acer truncatum seed essential oil prepared in comparative example 2 is shown in fig. 5.
Compared with the comparative example 2, the yield of the acer truncatum seed essential oil can be improved by 1.58%, and the relative content of alpha-bisabolol can be improved by 22.2%.
It can be seen that the present invention employs supercritical CO2The extraction can improve the yield of the acer truncatum seed essential oil and the relative content of functional components alpha-bisabolol in the essential oil, and compared with the conventional steam extraction, the yield of the essential oil and the relative content of the alpha-bisabolol are respectively improved by 1.58 percent and 22.2 percent.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The preparation method of the Acer truncatum seed essential oil is characterized by comprising the following steps:
step 1: taking Acer truncatum seeds, freeze-drying, crushing and sieving to obtain Acer truncatum seed coarse powder;
step 2: taking the Acer truncatum seed coarse powder obtained in the step 1, and performing supercritical CO treatment2Extracting, carrying out fractional purification by a separation kettle I and a separation kettle II, and collecting essential oil in the separation kettle II, namely the acer truncatum seed essential oil.
2. The method for preparing Acer truncatum seed essential oil according to claim 1, wherein in the step 1, the freeze drying temperature is-55 ℃ to-60 ℃ and the time is 24h to 48 h.
3. The method for preparing Acer truncatum seed essential oil according to claim 1, wherein in the step 1, the sieved aperture is 40 meshes.
4. The method for preparing Acer truncatum seed essential oil of claim 1, wherein the water content of the Acer truncatum seed meal in the step 1 is 4-6 wt%.
5. The method for preparing Acer acutangum seed essential oil according to any one of claims 1 to 4, wherein in the step 2, the supercritical CO is adopted2The specific parameters extracted are: by supercritical CO2Is solvent, ethanol with 95-100 vol% concentration is entrainer, the flow ratio of entrainer and solvent is 10-15%, and CO2The flow is 20L/h-30L/h, the extraction pressure is 12MPa-14MPa, the extraction temperature is 35-37 ℃, and the extraction time is 1h-2 h; the pressure of the separation kettle I is 8MPa-10MPa, and the temperature is 35-37 ℃; the pressure of the separation kettle II is 5MPa-7MPa, and the temperature is 31 ℃ to 33 ℃.
6. The Acer acutangum seed essential oil prepared by the method for preparing the Acer acutangum seed essential oil according to any one of claims 1 to 5.
7. The use of the Acer acutangum seed essential oil prepared by the method for preparing the Acer acutangum seed essential oil according to any one of claims 1 to 5 in the preparation of antibacterial and anti-inflammatory medicines.
8. The use of claim 7, wherein the anti-bacterial and anti-inflammatory agent comprises Acer acutus essential oil and a pharmaceutically acceptable carrier.
9. The use according to claim 7, wherein the pharmaceutically acceptable carrier is any one or more of a diluent, an absorption enhancer, an excipient, a surfactant, a binder, a wetting agent, a lubricant and a flavoring agent.
10. The use according to claim 7, wherein the antibacterial and anti-inflammatory drug is in a dosage form of any one of a solid preparation, a semi-solid preparation and a liquid preparation.
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| CN103007570A (en) * | 2012-12-10 | 2013-04-03 | 刘东波 | Integrated method for dehydrating traditional Chinese medicine materials, extracting essential oil and preparing essential oil |
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| CN102978005A (en) * | 2012-12-06 | 2013-03-20 | 重庆市日用化学工业研究所 | Method for extracting radix saussureae lappae essential oil by supercritical carbon dioxide |
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| CN104388192A (en) * | 2014-12-03 | 2015-03-04 | 湖北中烟工业有限责任公司 | Rose-type essence for cigarettes for women and application of flavor |
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