CN1127533A - 核酸的衔接线性扩增 - Google Patents

核酸的衔接线性扩增 Download PDF

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Publication number
CN1127533A
CN1127533A CN94192853A CN94192853A CN1127533A CN 1127533 A CN1127533 A CN 1127533A CN 94192853 A CN94192853 A CN 94192853A CN 94192853 A CN94192853 A CN 94192853A CN 1127533 A CN1127533 A CN 1127533A
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CN
China
Prior art keywords
primer
template
primer extension
generation
replicability
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN94192853A
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English (en)
Chinese (zh)
Inventor
罗伯特·布鲁斯·华莱士
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of CN1127533A publication Critical patent/CN1127533A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN94192853A 1993-07-23 1994-07-25 核酸的衔接线性扩增 Pending CN1127533A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9544293A 1993-07-23 1993-07-23
US08/095,442 1993-07-23

Publications (1)

Publication Number Publication Date
CN1127533A true CN1127533A (zh) 1996-07-24

Family

ID=22252059

Family Applications (1)

Application Number Title Priority Date Filing Date
CN94192853A Pending CN1127533A (zh) 1993-07-23 1994-07-25 核酸的衔接线性扩增

Country Status (5)

Country Link
EP (1) EP0710296A4 (fr)
JP (1) JPH09500539A (fr)
CN (1) CN1127533A (fr)
CA (1) CA2167840A1 (fr)
WO (1) WO1995003432A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335184B1 (en) 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
JPH11507235A (ja) * 1995-06-07 1999-06-29 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 核酸の連結型線形増幅法
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
DK0862656T3 (da) 1995-11-21 2001-04-09 Univ Yale Unimolekylær segmentamplifikation og -detektering
US6316229B1 (en) 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers
CA2342838A1 (fr) 1998-09-15 2000-03-23 Yale University Clonage moleculaire a amplification selon le modele du cercle roulant
JP2002525078A (ja) 1998-09-15 2002-08-13 イェール ユニバーシティ 人工的長末端反復配列
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
DE60130763T2 (de) 2000-06-30 2008-07-17 Qiagen Gmbh Signalamplifikation mit "lollipop"-hydridisierungsproben
US6573051B2 (en) 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
EP1762627A1 (fr) 2005-09-09 2007-03-14 Qiagen GmbH Procédé pour l'activation d'acides nucléiques pour effectuer une réaction d'une polymérase
EP2377929A1 (fr) 2005-10-06 2011-10-19 Lucigen Corporation Polymérases virales thermostables et procédés d'utilisation
JP6556728B2 (ja) * 2013-09-13 2019-08-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ポリＡ−キャリヤーにより誘導される高分子量ＰＣＲ生成物の生成を避けるためのオリゴ−ｄＴ分子の適用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
GB8920097D0 (en) * 1989-09-06 1989-10-18 Ici Plc Amplification processes

Also Published As

Publication number Publication date
JPH09500539A (ja) 1997-01-21
CA2167840A1 (fr) 1995-02-02
WO1995003432A1 (fr) 1995-02-02
EP0710296A1 (fr) 1996-05-08
EP0710296A4 (fr) 1999-05-26

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