WO1995003432A1 - Amplification lineaire enchainee d'acides nucleiques - Google Patents

Amplification lineaire enchainee d'acides nucleiques Download PDF

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Publication number
WO1995003432A1
WO1995003432A1 PCT/US1994/008390 US9408390W WO9503432A1 WO 1995003432 A1 WO1995003432 A1 WO 1995003432A1 US 9408390 W US9408390 W US 9408390W WO 9503432 A1 WO9503432 A1 WO 9503432A1
Authority
WO
WIPO (PCT)
Prior art keywords
primers
primer extension
primer
nucleic acid
process according
Prior art date
Application number
PCT/US1994/008390
Other languages
English (en)
Inventor
Robert Bruce Wallace
Original Assignee
Bio-Rad Laboratories, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio-Rad Laboratories, Inc. filed Critical Bio-Rad Laboratories, Inc.
Priority to EP94924010A priority Critical patent/EP0710296A4/fr
Priority to JP7505384A priority patent/JPH09500539A/ja
Publication of WO1995003432A1 publication Critical patent/WO1995003432A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Abstract

On effectue la synthèse extensive ('amplification') d'une séquence d'acide nucléique à étudier au moyen d'une série enchaînée de réactions d'extension d'amorces à plusieurs cycles. Les amorces utilisées dans chacune des réactions d'extension d'amorce du procédé contiennent des éléments non réplicables qui stoppent la synthèse d'acide nucléique et empêchent par conséquent les molécules synthétisées de servir de matrice dans les cycles subséquents. Les molécules synthétisées s'accumulent de manière mathématiquement linéaire pendant l'extension de l'amorce et confèrent au procédé une relative insensibilité par rapport aux acides nucléiques contaminants. Plusieurs ensembles d'amorces sont utilisés afin d'assurer l'accumulation d'un grand nombre de copies de la séquence d'acide nucléique à étudier. L'invention concerne également la détection d'une séquence d'acide nucléique à étudier ainsi que des trousses de réactifs permettant d'effectuer une telle réaction.
PCT/US1994/008390 1993-07-23 1994-07-25 Amplification lineaire enchainee d'acides nucleiques WO1995003432A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP94924010A EP0710296A4 (fr) 1993-07-23 1994-07-25 Amplification lineaire enchainee d'acides nucleiques
JP7505384A JPH09500539A (ja) 1993-07-23 1994-07-25 核酸のリンクされた線形増幅

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9544293A 1993-07-23 1993-07-23
US08/095,442 1993-07-23

Publications (1)

Publication Number Publication Date
WO1995003432A1 true WO1995003432A1 (fr) 1995-02-02

Family

ID=22252059

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/008390 WO1995003432A1 (fr) 1993-07-23 1994-07-25 Amplification lineaire enchainee d'acides nucleiques

Country Status (5)

Country Link
EP (1) EP0710296A4 (fr)
JP (1) JPH09500539A (fr)
CN (1) CN1127533A (fr)
CA (1) CA2167840A1 (fr)
WO (1) WO1995003432A1 (fr)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0832278A1 (fr) * 1995-06-07 1998-04-01 Bio-Rad Laboratories, Inc. Amplification lineaire liee d'acides nucleiques
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6143495A (en) * 1995-11-21 2000-11-07 Yale University Unimolecular segment amplification and sequencing
US6255082B1 (en) 1998-09-15 2001-07-03 Yale University Artificial long terminal repeat vectors
US6287824B1 (en) 1998-09-15 2001-09-11 Yale University Molecular cloning using rolling circle amplification
US6316229B1 (en) 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers
US6335184B1 (en) 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
EP1294933A2 (fr) * 2000-02-28 2003-03-26 Molecular Staging Inc. Procede de reduction des artefacts dans l'amplification d'acide nucleique
US6573051B2 (en) 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
US6686157B2 (en) 2000-06-30 2004-02-03 Molecular Staging Inc. Signal amplification with lollipop probes
US8093030B2 (en) 2005-10-06 2012-01-10 Lucigen Corporation Thermostable viral polymerases and methods of use
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US9683255B2 (en) 2005-09-09 2017-06-20 Qiagen Gmbh Method for activating a nucleic acid for a polymerase reaction

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6556728B2 (ja) * 2013-09-13 2019-08-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ポリＡ−キャリヤーにより誘導される高分子量ＰＣＲ生成物の生成を避けるためのオリゴ−ｄＴ分子の適用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
GB8920097D0 (en) * 1989-09-06 1989-10-18 Ici Plc Amplification processes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683195B1 (fr) * 1986-01-30 1990-11-27 Cetus Corp

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
INNIS et al., "Pcr Protocols", published 1990 by ACADEMIC PRESS INC. (N.Y.), pages 189-195, see entire document. *
See also references of EP0710296A4 *

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335184B1 (en) 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
EP0832278A1 (fr) * 1995-06-07 1998-04-01 Bio-Rad Laboratories, Inc. Amplification lineaire liee d'acides nucleiques
EP0832278A4 (fr) * 1995-06-07 1999-05-26 Bio Rad Laboratories Amplification lineaire liee d'acides nucleiques
US6210884B1 (en) 1995-11-21 2001-04-03 Yale University Rolling circle replication reporter systems
US6344329B1 (en) 1995-11-21 2002-02-05 Yale University Rolling circle replication reporter systems
US6143495A (en) * 1995-11-21 2000-11-07 Yale University Unimolecular segment amplification and sequencing
US6797474B2 (en) 1995-11-21 2004-09-28 Yale University Rolling circle replication reporter systems
US6632609B2 (en) 1995-11-21 2003-10-14 Yale University Unimolecular segment amplification and sequencing
US6183960B1 (en) 1995-11-21 2001-02-06 Yale University Rolling circle replication reporter systems
US6329150B1 (en) 1995-11-21 2001-12-11 Yale University Unimolecular segment amplification and sequencing
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6316229B1 (en) 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers
US6287824B1 (en) 1998-09-15 2001-09-11 Yale University Molecular cloning using rolling circle amplification
US6255082B1 (en) 1998-09-15 2001-07-03 Yale University Artificial long terminal repeat vectors
EP1294933A2 (fr) * 2000-02-28 2003-03-26 Molecular Staging Inc. Procede de reduction des artefacts dans l'amplification d'acide nucleique
US6686157B2 (en) 2000-06-30 2004-02-03 Molecular Staging Inc. Signal amplification with lollipop probes
US6573051B2 (en) 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US9683255B2 (en) 2005-09-09 2017-06-20 Qiagen Gmbh Method for activating a nucleic acid for a polymerase reaction
US8093030B2 (en) 2005-10-06 2012-01-10 Lucigen Corporation Thermostable viral polymerases and methods of use

Also Published As

Publication number Publication date
JPH09500539A (ja) 1997-01-21
CA2167840A1 (fr) 1995-02-02
EP0710296A4 (fr) 1999-05-26
EP0710296A1 (fr) 1996-05-08
CN1127533A (zh) 1996-07-24

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