CN112741000B - Vitex negundo young stem callus induction device - Google Patents
Vitex negundo young stem callus induction device Download PDFInfo
- Publication number
- CN112741000B CN112741000B CN202011604188.2A CN202011604188A CN112741000B CN 112741000 B CN112741000 B CN 112741000B CN 202011604188 A CN202011604188 A CN 202011604188A CN 112741000 B CN112741000 B CN 112741000B
- Authority
- CN
- China
- Prior art keywords
- sliding
- wall
- cabinet body
- block
- compression spring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a vitex negundo linn young stem callus induction device which comprises a cabinet body, wherein supporting legs are arranged on the outer side of the bottom wall of the cabinet body, and the device is characterized in that the cabinet body is internally divided into a left chamber, a middle chamber and a right chamber by two partition plates, a plurality of light-transmitting windows are arranged on the side wall of each partition plate, a plurality of equally-spaced horizontal sliding plates are longitudinally arranged in the left chamber and the right chamber, the horizontal sliding plates are in sliding connection with the inner wall of the cabinet body and the partition plates through sliding assemblies, a mounting sleeve is arranged on the upper surface of each horizontal sliding plate, a culture bottle is placed in the mounting sleeve, an induction culture medium is contained in the culture bottle, and vitex negundo linn young stem sections are placed on the induction culture medium. The young stem callus induction device of the negundo chastetree is beneficial to the production of negundo chastetree seedlings, promotes the artificial planting of the negundo chastetree and the cultivation of negundo chastetree forests, and promotes the comprehensive development and utilization of the negundo chastetree and the large-scale production and deep development of series products thereof.
Description
Technical Field
The invention belongs to the technical field of tissue culture, and particularly relates to a vitex negundo linn young stem callus induction device.
Background
Vitex negundo L also known as Vitex negundo L, Citrus quinquefolia, Toxicodendron quinquefolium, Zingiber officinale, and Nepeta arborescens of Vitex of Verbenaceae. China is commonly found in the south of the Yangtze river and in the Qinling mountain and Huaihe river in North China; shandong also has distribution; abroad through Mogara, southeast Asia and Bolivia in south America. Fructus Viticis negundo has effects of relieving cough, eliminating phlegm and relieving bronchospasm, and can be used for treating common cold, cough, asthma, rheumatism, malaria, stomach ache, hernia, and anal fistula. The folium Viticis negundo has effects of clearing heat, relieving exterior syndrome, promoting diuresis and removing toxic substance, and can be used for treating common cold, heatstroke, vomiting and diarrhea, dysentery, malaria, jaundice, rheumatism, traumatic injury swelling and pain, sore and scabies, and preventing the development of formaldehyde arthritis swelling. The fructus Viticis negundo and fructus Viticis negundo root decoction has inhibitory effect on Staphylococcus aureus and Catalpa, and fructus Viticis negundo is also recognized as effective Chinese herbal medicine by the Japanese medicine world, and has expectorant and tonic effects. The fruits (negundo chastetree seeds), roots, stems and leaves can be used as medicines according to records in Ben Cao gang mu and national Chinese herbal medicine compilation in China, but the fruits, the roots, the stems and the leaves are used in few areas, and only Hakkas have good use of custom. Hitherto, in Hakka areas such as Meizhou, the fruits are still used as pillows, and the leaves are used for replacing tea for guests or for gifting relatives and friends. The existing merchants develop series products by taking negundo chastetree as a material, and market is both in and out. In addition, the vitex negundo is also an excellent material for slope protection greening and water and soil conservation tree species, honey plants, aromatic insect-repellent plants, fiber plants and bonsai manufacturing, and has wide and important utilization value and huge development prospect.
Along with the continuous excavation of the development and utilization value of the negundo chastetree fruits by people, the social demand of the negundo chastetree fruits is increasingly expanded. The mode that the raw materials of the negundo chastetree series products are completely collected and supplied by wild resources cannot meet the requirement of market development. The artificial planting of the negundo chastetree fruits and the cultivation of the negundo chastetree forest are imperative. Therefore, a great deal of negundo chastetree seedlings are urgently needed to be propagated for market development.
Disclosure of Invention
Aiming at the defects, the invention provides the vitex negundo linn young stem callus induction device, which effectively improves vitex negundo linn propagation, provides theoretical basis and technical route for producing a large amount of high-quality vitex negundo linn seedlings, is beneficial to vitex negundo linn seedling production, and promotes vitex negundo linn artificial planting and vitex negundo linn forest cultivation.
The technical scheme adopted by the invention is as follows:
the utility model provides a vitex negundo young stem section callus induction device, the intelligent cabinet temperature adjusting device comprises a cabinet body, the diapire outside of the cabinet body is provided with the landing leg, the internal portion of cabinet has been cut apart into left cavity, middle cavity and right cavity by two baffles that set up with it, the lateral wall of baffle is provided with a plurality of light windows, vertically be provided with a plurality of equidistant horizontal sliding plate along it in left side cavity and the right cavity, horizontal sliding plate passes through slip subassembly and internal wall of cabinet and baffle sliding connection, horizontal sliding plate's upper surface is provided with the installation cover, the blake bottle has been placed in the installation cover, the splendid attire has the induction culture medium in the blake bottle, and vitex young stem section is placed on the induction culture medium.
Preferably, the sliding assembly comprises a supporting table arranged on the inner wall of the cabinet body and the side wall of the partition board, a first sliding groove is formed in the supporting table, a sliding rod is arranged in the first sliding groove, second sliding grooves are formed in two sides of the lower surface of the horizontal sliding plate, a first sliding block and a second sliding block are arranged in the second sliding groove in a sliding mode, the lower ends of the first sliding block and the second sliding block extend out of the second sliding groove and are sleeved on the sliding rod, a first compression spring is arranged between the first sliding block and the second sliding block, a second compression spring is arranged between the first sliding block and the inner wall of the first sliding groove, and the first compression spring and the second compression spring are all sleeved on the sliding rod.
Preferably, the sliding assembly further comprises a third sliding groove, first balls and V-shaped pulleys, the third sliding groove is formed in the inner wall of the cabinet body and the side wall of the partition board, the two sides of the horizontal sliding plate are located in the third sliding groove, a first guide groove is formed in the inner side wall of the third sliding groove inwards, the first balls and the V-shaped pulleys are arranged in a plurality of the first guide grooves in a distributed mode, and the first balls are in sliding contact with the side wall of the horizontal sliding plate.
Preferably, two ends of the first compression spring are fixedly connected with the first sliding block and the second sliding block respectively.
Preferably, cabinet body roof inboard is provided with liquid container, and its outside is provided with header tank and atomizer, the header tank passes through the outlet pipe and is connected with the atomizer, be provided with the control valve on the outlet pipe, the atomizer passes through the catheter and is connected with liquid container, cabinet body diapire inboard is provided with the box body, be provided with the gas guide tube between box body and the liquid container, the one end of gas guide tube extends to inside the liquid container, and its other end extends to inside and being connected with drive assembly of box body, liquid container, gas guide tube and box body all are located the middle cavity, be provided with the branch road pipe of symmetric distribution on the gas guide tube, the tip of branch road pipe is connected with the shower nozzle through the chucking subassembly.
Preferably, an air inlet groove is formed in the upper end face of the air guide cylinder, the air inlet groove extends downwards to the upper end face of the box body along the height direction of the air guide cylinder, the air inlet groove is communicated with the branch pipe, a fixed block is arranged inside the branch pipe, the fixed block is connected with the inner wall of the branch pipe through a connecting rod, the inside of the fixed block is of a hollow structure, a guide block is arranged inside the fixed block, two ends of the guide block are respectively sleeved on the guide rod, two ends of the guide rod are fixedly connected with the left inner wall and the right inner wall of the fixed block, a third compression spring is arranged between the guide block and the left inner wall of the fixed block, the third compression spring is sleeved on the guide rod, and an opening is formed in the right inner wall of the fixed block;
the side wall of the branch pipe is provided with a through hole, a fixture block is arranged in the through hole, the bottom of the fixture block extends into the inside of the branch pipe and penetrates through the outer wall of the mounting seat, the bottom of the fixture block is provided with a fixing plate, the fixing plate is connected with the guide block through a spring steel sheet, the mounting seat is fixedly connected to the inner side wall of the branch pipe, the inner cavity of the mounting seat is provided with a fourth compression spring, the fourth compression spring is sleeved on the fixture block, a clamping hole matched with the fixture block is formed in the side wall of the spray head, and the end face of the spray head is provided with a horn-shaped spray hole.
Preferably, an inclined surface is arranged on one side, facing the spray head, of the top of the fixture block.
Preferably, the driving assembly comprises a sleeve ring, a thrust ball bearing, an annular guide rail and a support rod, the sleeve ring is fixed on the bottom wall of the cabinet body through a bolt, the thrust ball bearing is arranged at the lower end of the gas cylinder and is installed at the top of the sleeve ring, the annular guide rail is arranged on the bottom wall of the cabinet body around the sleeve ring, the lower end of the support rod is located in a fourth sliding groove in the annular guide rail, the upper end of the support rod is connected with a rotary table arranged on the gas cylinder, second guide grooves are formed in two side walls of the fourth sliding groove, and second balls are arranged in the second guide grooves; the supporting rod is provided with a mounting plate, the mounting plate is provided with a motor, an output shaft of the motor is provided with a gear, and the gear is meshed with an annular rack arranged on the inner wall of the box body.
Preferably, a sealing base plate is arranged at the upper end of the gas cylinder and is positioned on the inner side of the bottom wall of the liquid container, a sealing ring is arranged on the liquid guide pipe and is positioned on the outer side of the top wall of the cabinet body.
Preferably, the inner side of the top wall of the cabinet body is also provided with an LED lamp, and the LED lamp is close to the front side wall and the rear side wall of the cabinet body; the cabinet is internal still to be provided with humidity transducer and temperature sensor, humidity transducer, temperature sensor and LED lamp all with control panel electric connection, control panel sets up on the lateral wall of the cabinet body.
The invention has the beneficial effects that: according to the device for inducing the calluses of the young stem segments of the negundo chastetree, the temperature and the humidity in the cabinet body are monitored in real time through the temperature sensor and the humidity sensor, a good environment is provided for inducing the calluses of the young stem segments of the negundo chastetree, and the humidity in the cabinet body is adjusted through the cooperation of the air guide cylinder, the branch pipe and the spray head; secondly, the LED lamp is arranged in the cabinet body, and the illumination intensity in the cabinet body is adjusted to ensure the illumination condition required by the callus induction of the young stem segments of the negundo chastetree.
The invention also designs a driving assembly which comprises a lantern ring, a thrust ball bearing, an annular guide rail and a support rod, wherein the support rod is used for realizing the connection of a turntable on the gas guide cylinder and the annular guide rail, and the gear is driven by a motor to do circular motion along the annular rack on the inner wall of the box body so as to drive the support rod to move around the annular guide rail, realize the self transmission of the gas guide cylinder, spray atomized water to corners in the cabinet body and further adjust the humidity in the cabinet body.
The invention also designs a sliding assembly which comprises a supporting table, a first sliding groove, a sliding rod, a second sliding groove, a first sliding block and a second sliding block, wherein the first sliding block and the second sliding block move along the sliding rod, so that the horizontal sliding plate can be conveniently moved out of the cabinet body, and the propagation condition of the callus in the culture bottle can be conveniently observed; in order to prolong the service life of the sliding assembly, the third sliding groove, the first ball and the V-shaped pulley are designed, and the sliding contact is formed between the first ball and the side wall of the horizontal sliding plate, so that the sliding portability of the horizontal sliding plate is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is an external view of a device for inducing callus of young stem segments of Vitex negundo L.according to the present invention;
FIG. 2 is an internal view of a device for inducing callus of young stem segments of Vitex negundo L.according to the present invention;
FIG. 3 is a side view of the separator plate;
FIG. 4 is a bottom view of the horizontal slide;
FIG. 5 is a side view of the horizontal slide and support platform;
FIG. 6 is a partial side view of the cabinet;
FIG. 7 is an enlarged view taken at A in FIG. 2;
FIG. 8 is an enlarged view at B in FIG. 2;
FIG. 9 is an enlarged view at C of FIG. 8;
FIG. 10 is a cross-sectional view of the endless track;
FIG. 11 is a cross-sectional view of the bypass tube and the showerhead.
Wherein, 1-cabinet body; 101-a third sliding groove; 102-a first guide slot; 103-a first ball; 104-V type pulley; 2-a separator; 201-light-transmitting window; 3-horizontal sliding plate; 301-a second sliding channel; 302-a first slider; 303-a second slider; 304-mounting the sleeve; 4-supporting the table; 401-a first sliding groove; 402-a slide bar; 403-a first compression spring; 404-a second compression spring; 5-culture flask; 6-gas cylinder; 601-a turntable; 7-branch pipes; 701-fixing block; 702-a linkage; 703-a guide bar; 704-a third compression spring; 705-a guide block; 706-spring steel sheet; 707-fixing plate; 708-a mount; 709-fixture block; 710-a fourth compression spring; 8-a spray head; 801-card hole; 802-trumpet-shaped spray holes; 9-a box body; 901-ring rack; 10-liquid container; 11-a collar; 12-a thrust ball bearing; 13-a ring guide; 1301-a fourth sliding groove; 1302-a second guide slot; 1303 — a second ball; 14-a strut; 15-mounting a plate; 16-an electric machine; 17-a gear; 18-a water collection tank; 19-an atomizer; 20-a catheter; 21-an LED lamp; 22-a control panel; 23-support leg.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention specifically provides a vitex negundo linn young stem callus induction device, as shown in fig. 1-11, comprising a cabinet body 1, wherein support legs 23 are arranged on the outer side of the bottom wall of the cabinet body 1, the cabinet body 1 is internally divided into a left chamber, a middle chamber and a right chamber by two partition plates 2, a plurality of light-transmitting windows 201 are arranged on the side walls of the partition plates 2, a plurality of equally-spaced horizontal sliding plates 3 are longitudinally arranged in the left chamber and the right chamber, the horizontal sliding plates 3 are in sliding connection with the inner wall of the cabinet body 1 and the partition plates 2 through sliding components, an installation sleeve 304 is arranged on the upper surface of the horizontal sliding plates 3, a culture bottle 5 is placed in the installation sleeve 304, an induction culture medium is contained in the culture bottle 5, and vitex negundo linn young stem sections are placed on the induction culture medium.
In this embodiment, as shown in fig. 3-7, in order to facilitate the moving of the horizontal sliding plate 3 out of the cabinet and the placing, taking out and observing of the callus propagation of young stem segments of vitex negundo, the sliding assembly includes a supporting platform 4 disposed on the inner wall of the cabinet 3 and the side wall of the partition board 2, a first sliding groove 401 is disposed on the supporting platform 4, a sliding rod 402 is disposed inside the first sliding groove 401, second sliding grooves 301 are disposed on two sides of the lower surface of the horizontal sliding plate 3, a first sliding block 302 and a second sliding block 303 are slidably disposed in the second sliding groove 301, the lower ends of the first sliding block 302 and the second sliding block 303 extend out of the second sliding groove 301 and are sleeved on the sliding rod 402, a first compression spring 403 is disposed between the first sliding block 302 and the second sliding block 303, a second compression spring 404 is disposed between the first sliding block 303 and the inner wall of the first sliding groove 401, the first compression spring 403 and the second compression spring 404 are sleeved on the sliding rod 402, and two ends of the first compression spring 403 are respectively and fixedly connected with the first sliding block 302 and the second sliding block 303.
Simultaneously, in order to improve horizontal sliding plate 3's stability and sliding assembly's life, sliding assembly still includes third sliding tray 101, first ball 103 and V type pulley 104, third sliding tray 101 sets up on cabinet body 1 inner wall and 2 lateral walls of baffle, horizontal sliding plate 3's both sides are located third sliding tray 101, first guide slot 102 has been seted up inwards to third sliding tray 101 inside wall, first ball 103 and V type pulley 104 are provided with a plurality ofly and distribute in first guide slot 102 in turn, first ball 103 and 3 lateral walls sliding contact of horizontal sliding plate reduce the resistance that horizontal sliding plate 3 lateral wall received.
When the horizontal sliding plate 3 slides, the first sliding block 302 and the second sliding block 303 move along the sliding rod 402, because the first sliding block 302 and the second sliding block 303 can also slide in the second sliding groove 301, therefore, when the first sliding block 302 slides to the foremost end, the second compression spring 404 is compressed to the maximum, and simultaneously, the second sliding block 303 can also move along the sliding rod, when the second sliding block 303 moves to the foremost end, the first compression spring 403 between the first sliding block 302 and the second sliding block 303 is compressed to the maximum, so that the installation sleeve 304 on the horizontal sliding plate 3 is completely moved to the outer side of the cabinet body 1, the culture bottle 5 is convenient to place and take out, meanwhile, the callus propagation condition of the young stem segments of the vitex negundo is convenient to observe, and the recording is convenient.
In this embodiment, in order to adjust the temperature, humidity and illumination intensity inside the cabinet body 1, the inner side of the top wall of the cabinet body is further provided with an LED lamp 21, and the LED lamp 21 is close to the front and rear side walls of the cabinet body 1; the cabinet is internal still to be provided with humidity transducer and temperature sensor (not shown in the figure), humidity transducer, temperature sensor and LED lamp 21 all with control panel 22 electric connection, control panel 22 sets up on the lateral wall of the cabinet body 1. The temperature and humidity and the illumination condition inside the cabinet body 1 are monitored in real time through the control panel 22. The conditions for inducing the vitex negundo L young stem callus are as follows: the culture temperature is 25 ℃; the illumination intensity is 2000 lx; the relative humidity is 50-70%.
In order to adjust the humidity inside the cabinet body 1, as shown in fig. 11, the cabinet body top wall is provided with a liquid container on the inner side, the outer side of the cabinet body top wall is provided with a water collecting tank 18 and an atomizer 19, the water collecting tank 18 is connected with the atomizer 19 through a water outlet pipe, a control valve is arranged on the water outlet pipe, the atomizer 19 is connected with the liquid container 10 through a liquid guide pipe 20, the inner side of the bottom wall of the cabinet body 1 is provided with a box body 9, an air guide cylinder 6 is arranged between the box body 9 and the liquid container 10, one end of the air guide cylinder 6 extends to the inside of the liquid container 10, the other end of the air guide cylinder extends to the inside of the box body 9 and is connected with a driving component, the liquid container 10, the air guide cylinder 6 and the box body 9 are all located in a middle chamber, branch pipes 7 which are symmetrically distributed are arranged on the air guide cylinder 6, and the end parts of the branch pipes 7 are connected with a spray head 8 through a clamping component.
The gas guide cylinder 6 is provided with an air inlet groove on the upper end surface, the air inlet groove extends downwards to the upper end surface of the box body 9 along the height direction of the gas guide cylinder, the air inlet groove is communicated with the branch pipe 7, the fixed block 701 is arranged in the branch pipe 7, the fixed block 701 is connected with the inner wall of the branch pipe 7 through the connecting rod 702, the fixed block 701 is of a hollow structure, the guide block 705 is arranged in the fixed block 701, the two ends of the guide block 705 are respectively sleeved on the guide rods 703, the two ends of the guide rods 703 are fixedly connected with the left inner wall and the right inner wall of the fixed block 701, a third compression spring 704 is arranged between the guide block 705 and the left inner wall of the fixed block 701, the third compression spring 703 is sleeved on the guide rods 703, and the right inner wall of the fixed block 701 is provided with an opening; a through hole is formed in the side wall of the branch pipe 7, a fixture block 709 is arranged in the through hole, and an inclined surface is arranged on one side, facing the spray head 8, of the top of the fixture block 709; the bottom of the fixture block 709 extends into the branch pipe 7 and penetrates through the outer wall of the mounting seat 708, a fixing plate 707 is arranged at the bottom of the fixture block 709, the fixing plate 707 is connected with the guide block 705 through a spring steel sheet 706, the mounting seat 708 is fixedly connected to the inner side wall of the branch pipe 7, a fourth compression spring 710 is arranged in the inner cavity of the mounting seat 708, the fourth compression spring 710 is sleeved on the fixture block, a fixture hole 801 matched with the fixture block 709 is formed in the side wall of the spray head 8, and a horn-shaped spray hole 802 is formed in the end face of the spray head 8.
Wherein, the upper end of the gas cylinder 6 is provided with a sealing base plate, the sealing base plate is positioned on the inner side of the bottom wall of the liquid container 10, the liquid guide pipe 20 is provided with a sealing ring, and the sealing ring is positioned on the outer side of the top wall of the cabinet body 1.
In this embodiment, as shown in fig. 8 to 10, the driving assembly includes a collar 11, a thrust ball bearing 12, an annular guide rail 13, and a support rod 14, the collar 11 is fixed on the bottom wall of the cabinet 1 by bolts, the thrust ball bearing 12 is disposed at the lower end of the gas cylinder 6 and is mounted on the top of the collar 11, the annular guide rail 13 is disposed on the bottom wall of the cabinet 1 around the collar 11, the lower end of the support rod 14 is located in a fourth sliding groove 1301 on the annular guide rail 13, the upper end of the support rod is connected with a rotating disc disposed on the gas cylinder 6, second guide grooves 1302 are disposed on two side walls of the fourth sliding groove 1301, and second balls 1303 are disposed in the second guide grooves 1302; the utility model discloses a box body 9, including branch 14, motor 16, gear 17, mounting panel 15, motor 16 and commercial power connection, be provided with mounting panel 15 on the branch 14, be provided with motor 16 on the mounting panel 15, motor 16's output shaft is provided with gear 17, gear 17 meshes with the annular rack 901 that sets up on the box body 9 inner wall.
When the humidity in the cabinet 1 needs to be adjusted, the motor 16 is turned on, the driving gear 17 rotates around the annular rack 901, the supporting rod is driven to slide in the fourth sliding groove 1301 on the annular guide rail 13, the air guide cylinder 6 is driven to rotate through the rotary disc 601, atomized water vapor is sprayed out through the spray head 8 along the branch pipe 7, and therefore the humidity in the cabinet 1 is increased.
When in use, an induction culture medium is prepared at present, an MS culture medium is adopted as a basic culture medium, and the culture medium contains 0.9% of agar and 3% of sucrose; the pH value is 5.8-6.0 before sterilization; autoclaving at 121 deg.C for 20 min; 1.5. mu.M TDZ + 0.50. mu.M KT was added. Invert the culture flask with the culture medium in, and place the tissue on the culture medium, then put the culture flask in the installation cover, it is internal to push back the cabinet with horizontal slide again, the cabinet body will carry out sterilization before using, and dry, in the use, open control panel, through the humiture of the internal portion of temperature sensor and humidity transducer real-time supervision cabinet, when humidity is less than cultivates required humidity, then open motor drive gear and rotate round annular rack, it slides to drive branch fourth sliding tray on ring rail, and drive the rotation of air guide tube through the carousel, aqueous vapor after the atomizing passes through the shower nozzle blowout along the branch road pipe, thereby increase the humidity of the internal portion of cabinet. And controlling the LED to be turned on and off according to the illumination period of 12h light/12 h dark. Inducing culture for at least 3 days.
The above description is only for the purpose of illustrating the technical solutions of the present invention and not for the purpose of limiting the same, and other modifications or equivalent substitutions made by those skilled in the art to the technical solutions of the present invention should be covered within the scope of the claims of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (6)
1. A callus induction device for young stems of negundo chastetree comprises a cabinet body, wherein supporting legs are arranged on the outer side of the bottom wall of the cabinet body, and the callus induction device is characterized in that the cabinet body is divided into a left chamber, a middle chamber and a right chamber by two partition plates, a plurality of light transmission windows are arranged on the side walls of the partition plates, a plurality of equally spaced horizontal sliding plates are longitudinally arranged in the left chamber and the right chamber, the horizontal sliding plates are in sliding connection with the inner wall of the cabinet body and the partition plates through sliding assemblies, a mounting sleeve is arranged on the upper surface of each horizontal sliding plate, a culture bottle is placed in each mounting sleeve, an induction culture medium is contained in each culture bottle, and young stems of negundo chastetree are placed on the induction culture medium;
the improved water dispenser is characterized in that a liquid container is arranged on the inner side of the top wall of the cabinet body, a water collecting tank and an atomizer are arranged on the outer side of the top wall of the cabinet body, the water collecting tank is connected with the atomizer through a water outlet pipe, a control valve is arranged on the water outlet pipe, the atomizer is connected with the liquid container through a liquid guide pipe, a box body is arranged on the inner side of the bottom wall of the cabinet body, an air guide cylinder is arranged between the box body and the liquid container, one end of the air guide cylinder extends into the liquid container, the other end of the air guide cylinder extends into the box body and is connected with a driving assembly, the liquid container, the air guide cylinder and the box body are all located in a middle cavity, symmetrically distributed branch pipes are arranged on the air guide cylinder, and the end parts of the branch pipes are connected with nozzles through clamping assemblies;
the upper end surface of the gas cylinder is provided with a gas inlet groove, the gas inlet groove extends downwards to the upper end surface of the box body along the height direction of the gas cylinder, the gas inlet groove is communicated with the branch pipe, a fixed block is arranged inside the branch pipe, the fixed block is connected with the inner wall of the branch pipe through a connecting rod, the inside of the fixed block is of a hollow structure, a guide block is arranged inside the fixed block, two ends of the guide block are respectively sleeved on the guide rod, two ends of the guide rod are fixedly connected with the left inner wall and the right inner wall of the fixed block, a third compression spring is arranged between the guide block and the left inner wall of the fixed block, the third compression spring is sleeved on the guide rod, and the right inner wall of the fixed block is provided with an opening;
the side wall of the branch pipe is provided with a through hole, a fixture block is arranged in the through hole, the bottom of the fixture block extends into the branch pipe and penetrates through the outer wall of the mounting seat, the bottom of the fixture block is provided with a fixed plate, the fixed plate is connected with the guide block through a spring steel sheet, the mounting seat is fixedly connected to the inner side wall of the branch pipe, the inner cavity of the mounting seat is provided with a fourth compression spring, the fourth compression spring is sleeved on the fixture block, the side wall of the spray head is provided with a clamping hole matched with the fixture block, and the end face of the spray head is provided with a horn-shaped spray hole;
an inclined surface is arranged on one side, facing the spray head, of the top of the clamping block;
the driving assembly comprises a sleeve ring, a thrust ball bearing, an annular guide rail and a support rod, the sleeve ring is fixed on the bottom wall of the cabinet body through a bolt, the thrust ball bearing is arranged at the lower end of the air guide cylinder and is installed at the top of the sleeve ring, the annular guide rail is arranged on the bottom wall of the cabinet body around the sleeve ring, the lower end of the support rod is located in a fourth sliding groove on the annular guide rail, the upper end of the support rod is connected with a rotary table arranged on the air guide cylinder, second guide grooves are formed in two side walls of the fourth sliding groove, and second balls are arranged in the second guide grooves; the supporting rod is provided with a mounting plate, the mounting plate is provided with a motor, an output shaft of the motor is provided with a gear, and the gear is meshed with an annular rack arranged on the inner wall of the box body.
2. The young vitex negundo stem callus induction device according to claim 1, wherein the sliding assembly comprises a supporting platform disposed on the inner wall of the cabinet and the side wall of the partition, the supporting platform is provided with a first sliding groove, a sliding rod is disposed inside the first sliding groove, second sliding grooves are disposed on two sides of the lower surface of the horizontal sliding plate, a first sliding block and a second sliding block are slidably disposed in the second sliding groove, the lower ends of the first sliding block and the second sliding block extend out of the second sliding groove and are sleeved on the sliding rod, a first compression spring is disposed between the first sliding block and the second sliding block, a second compression spring is disposed between the first sliding block and the inner wall of the first sliding groove, and the first compression spring and the second compression spring are both sleeved on the sliding rod.
3. The apparatus for inducing callus on young stem of negundo chastetree according to claim 2, wherein the sliding assembly further comprises a third sliding groove, a first ball and a V-shaped pulley, the third sliding groove is disposed on the inner wall of the cabinet and the sidewall of the partition, two sides of the horizontal sliding plate are disposed in the third sliding groove, a first guiding groove is inwardly disposed on the inner sidewall of the third sliding groove, the first ball and the V-shaped pulley are disposed in a plurality and alternately distributed in the first guiding groove, and the first ball is in sliding contact with the sidewall of the horizontal sliding plate.
4. The apparatus for inducing callus of young stem of negundo chastetree according to claim 2, wherein the two ends of the first compression spring are fixedly connected to the first slider and the second slider respectively.
5. The apparatus for inducing callus of young stem of negundo chastetree according to claim 1, wherein the gas cylinder is provided at its upper end with a sealing pad inside the bottom wall of the liquid container, and the liquid guiding tube is provided with a sealing ring outside the top wall of the cabinet.
6. The apparatus for inducing the calluses of young vitex negundo stems according to claim 1, wherein the inside of the top wall of the cabinet body is further provided with LED lamps, and the LED lamps are close to the front and rear side walls of the cabinet body; the cabinet is internal still to be provided with humidity transducer and temperature sensor, humidity transducer, temperature sensor and LED lamp all with control panel electric connection, control panel sets up on the lateral wall of the cabinet body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011604188.2A CN112741000B (en) | 2020-12-29 | 2020-12-29 | Vitex negundo young stem callus induction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011604188.2A CN112741000B (en) | 2020-12-29 | 2020-12-29 | Vitex negundo young stem callus induction device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112741000A CN112741000A (en) | 2021-05-04 |
| CN112741000B true CN112741000B (en) | 2022-02-01 |
Family
ID=75647178
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011604188.2A Active CN112741000B (en) | 2020-12-29 | 2020-12-29 | Vitex negundo young stem callus induction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112741000B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115041419B (en) * | 2022-06-13 | 2024-01-23 | 嘉应学院 | Spherical fruit azimuth detecting and adjusting device based on image recognition |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04287624A (en) * | 1991-03-19 | 1992-10-13 | Komatsu Ltd | Device for cutting and transplanting cultured plant |
| CN205658161U (en) * | 2016-05-31 | 2016-10-26 | 南阳师范学院 | Observe pti4 gene tissue culture case of kidney bean cotyledon festival explant system |
| CN206024781U (en) * | 2016-08-22 | 2017-03-22 | 西双版纳增靓生物科技有限公司 | Dendrobii officmalis caulis group banks up seedling with earth and cultivates rack |
| CN209480368U (en) * | 2018-12-04 | 2019-10-11 | 广州信源电力科技有限公司 | A kind of constant temperature dehumidification tool box |
| CN210017342U (en) * | 2019-05-17 | 2020-02-07 | 云南祥琬生物科技有限公司 | Humidity-adjustable cultivation device for tissue culture seedling planting |
| CN210808492U (en) * | 2019-08-02 | 2020-06-23 | 扬州市三发电子有限公司 | Intelligent climate box |
-
2020
- 2020-12-29 CN CN202011604188.2A patent/CN112741000B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN112741000A (en) | 2021-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112741000B (en) | Vitex negundo young stem callus induction device | |
| CN112136576A (en) | Seedling raising device for forsythia suspense seedlings | |
| CN103858760B (en) | The preparation method of a kind of roxburgh anoectochilus terminal bud test tube flowers | |
| CN207531484U (en) | A kind of storage device of bletilla seed stems | |
| CN108142149A (en) | A kind of Chinese herbal medicine plantation nursery frame plate | |
| CN211020330U (en) | Ganoderma lucidum spore collecting device | |
| CN208029832U (en) | A kind of Domestic two vegetable garden growth case | |
| CN110250004B (en) | Tissue culture and rapid propagation method for Stephania delavayi Diels | |
| CN207911877U (en) | A kind of tomato in plastic greenhouse sterilization and disinfestation device | |
| CN208972190U (en) | A kind of mushroom culturing room | |
| CN214126250U (en) | Forage millet fast germination device | |
| CN112369280A (en) | Automatic culture equipment and culture method suitable for antrodia camphorata | |
| CN216650680U (en) | Cedrela sinensis seedling is transplanted and is encircleed canopy with humidity automatically regulated | |
| CN220859036U (en) | Transplanting field planting equipment for pearl ginseng seedlings | |
| CN221011029U (en) | Cultivation case is used to traditional chinese medicine tuber of hyacinth bletilla | |
| CN112741001B (en) | Vitex negundo callus induction method | |
| CN109105247A (en) | A kind of open air solid soilless culturing device and its cultural method | |
| CN112166943B (en) | Support arrangement is planted to afforestation seedling | |
| CN111436313B (en) | Greenhouse temperature control device for industrial hemp planting | |
| CN207011363U (en) | A kind of plant protection device with windproof function | |
| CN219421690U (en) | Honeysuckle seedling culture rack convenient to watering | |
| CN214508624U (en) | Dendrobium nobile combined planting box | |
| CN112493137B (en) | Rapid propagation method for test-tube plantlets of negundo chastetree | |
| CN218868790U (en) | Greenhouse medicinal industrial hemp culture apparatus | |
| CN219330185U (en) | Novel rice incubator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |