CN112730397A - Test paper for rapidly determining hydrogen peroxide content and preparation method thereof - Google Patents
Test paper for rapidly determining hydrogen peroxide content and preparation method thereof Download PDFInfo
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- 238000012360 testing method Methods 0.000 title claims abstract description 134
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 title claims abstract description 127
- 238000002360 preparation method Methods 0.000 title abstract description 5
- 238000001514 detection method Methods 0.000 claims abstract description 58
- 239000000758 substrate Substances 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 23
- 239000003054 catalyst Substances 0.000 claims abstract description 18
- 239000000376 reactant Substances 0.000 claims abstract description 15
- 229920000642 polymer Polymers 0.000 claims abstract description 13
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 7
- 238000002791 soaking Methods 0.000 claims abstract description 5
- 238000001035 drying Methods 0.000 claims abstract description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical group [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims description 39
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 13
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 13
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 13
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 7
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 7
- MEFBJEMVZONFCJ-UHFFFAOYSA-N molybdate Chemical group [O-][Mo]([O-])(=O)=O MEFBJEMVZONFCJ-UHFFFAOYSA-N 0.000 claims description 7
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 7
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 6
- 239000011609 ammonium molybdate Substances 0.000 claims description 6
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 6
- 229940010552 ammonium molybdate Drugs 0.000 claims description 6
- QTYWBJZOZDYCGB-UHFFFAOYSA-L potassium;sodium;2-carboxybenzoate;hydroxide Chemical compound [OH-].[Na+].[K+].OC(=O)C1=CC=CC=C1C([O-])=O QTYWBJZOZDYCGB-UHFFFAOYSA-L 0.000 claims description 6
- 239000002202 Polyethylene glycol Substances 0.000 claims description 5
- 229940099596 manganese sulfate Drugs 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical group [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 229920001223 polyethylene glycol Polymers 0.000 claims description 5
- SPOMEWBVWWDQBC-UHFFFAOYSA-K tripotassium;dihydrogen phosphate;hydrogen phosphate Chemical compound [K+].[K+].[K+].OP(O)([O-])=O.OP([O-])([O-])=O SPOMEWBVWWDQBC-UHFFFAOYSA-K 0.000 claims description 5
- 150000002696 manganese Chemical class 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 3
- 239000011572 manganese Substances 0.000 claims description 3
- 229910052748 manganese Inorganic materials 0.000 claims description 3
- 235000002908 manganese Nutrition 0.000 claims description 3
- 239000003002 pH adjusting agent Substances 0.000 claims description 3
- 239000011684 sodium molybdate Substances 0.000 claims description 2
- 235000015393 sodium molybdate Nutrition 0.000 claims description 2
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 2
- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 claims description 2
- 238000005520 cutting process Methods 0.000 abstract description 16
- 239000000126 substance Substances 0.000 abstract description 6
- 238000004448 titration Methods 0.000 abstract description 5
- 239000012286 potassium permanganate Substances 0.000 abstract description 3
- 238000011005 laboratory method Methods 0.000 abstract description 2
- 238000002156 mixing Methods 0.000 abstract description 2
- 239000004753 textile Substances 0.000 abstract description 2
- 238000011161 development Methods 0.000 description 35
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- 230000000694 effects Effects 0.000 description 23
- 239000011259 mixed solution Substances 0.000 description 14
- 239000012086 standard solution Substances 0.000 description 14
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 10
- 239000004800 polyvinyl chloride Substances 0.000 description 10
- 229920000915 polyvinyl chloride Polymers 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 239000002250 absorbent Substances 0.000 description 8
- 230000002745 absorbent Effects 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 6
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 102000003992 Peroxidases Human genes 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 125000004429 atom Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- OZECDDHOAMNMQI-UHFFFAOYSA-H cerium(3+);trisulfate Chemical compound [Ce+3].[Ce+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O OZECDDHOAMNMQI-UHFFFAOYSA-H 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- 229910001448 ferrous ion Inorganic materials 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000005558 fluorometry Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
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- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
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Abstract
The invention relates to a test paper for rapidly determining hydrogen peroxide content and a preparation method thereof. Mixing a color developing agent, a polymer, a catalyst and a pH regulator to prepare a detection reactant solution, soaking qualitative filter paper in the detection reactant solution for 1-5 minutes, taking out the solution, drying the solution in an oven at 50-60 ℃, cutting the solution, and sticking the cut solution on a test paper substrate to obtain the test paper. Compared with the prior art, the test paper has reasonable structure, can be produced in batches, changes a solution method into a test paper method, does not need specific environment and professional operating skills, can realize rapid detection, and is convenient for civil use; compared with the common laboratory methods, the method has the advantages that the dosage of the test reagent is less (such as potassium permanganate titration method, indirect iodometry method and the like), the test cost is saved, and the method is widely applicable to the fields of chemical industry, textile industry, environment, laboratories and the like.
Description
Technical Field
The invention belongs to the technical field of peroxide detection, and particularly relates to test paper for rapidly determining hydrogen peroxide content and a preparation method thereof.
Background
Hydrogen peroxide (H)2O2) The aqueous solution is commonly called as hydrogen peroxide, has the functions of sterilization, disinfection, bleaching and the like, and is widely applied to the fields of chemical industry, textile, environment, food and the like. For example, in the treatment of wastewater, one of the main treatment methods is to degrade a water body by Fenton reactionThe organic pollutant in (1) is structurally changed by the strong oxidizing ability of hydrogen peroxide under the action of acidic conditions and ferrous ions. In the aspect of sterilization and disinfection, hydrogen peroxide is easy to decompose and release monatomic oxygen due to the instability of the structure, and the released oxygen atoms have strong oxidizing capability and can predate electrons and atoms of bacteria and microorganisms, so that the protein structure of the hydrogen peroxide is damaged to generate a denaturation effect, and the sterilization and disinfection effects are achieved.
Most of the currently used methods for detecting the hydrogen peroxide content include chemical titration, spectrophotometry, fluorometry, chromatography, electrochemical analysis, and the like. The chemical titration method comprises a potassium permanganate titration method, an indirect iodometry method and a cerium sulfate method, for example, the potassium permanganate titration method is applied in the method for measuring the hydrogen peroxide content in the industrial hydrogen peroxide national standard GB/T1616-2014. Chinese patent CN201710960593.X discloses a fluorescent probe for detecting hydrogen peroxide in a water sample. Chinese patent CN02158703.5 discloses a method for determining hydrogen peroxide content by high performance liquid chromatography. The methods have respective advantages, but have certain defects, some methods may need professional equipment, large-scale instruments and professional operators, and some methods are complex in operation, long in time consumption, complex in equipment structure and high in cost. Therefore, starting from a civil view, the solution method is converted into the test paper method, and the establishment of the detection method which is simple to operate, has a wide application range and can meet the requirement of quickly detecting the effective content of the hydrogen peroxide is very necessary.
In chinese patent CN87106131.7, potassium iodide paper method is used to make the analysis of peracetic acid fast and convenient, but it has poor stability, is not easy to store, and has low accuracy in determining its content by reaction time difference alone.
Chinese patent CN201220408884 discloses a low concentration residual peroxyacetic acid test paper, which comprises a test paper, test paper one end is handheld district, and the other end is equipped with the reaction zone, be equipped with the peroxidase reactant of test peroxyacetic acid on the reaction zone, the utility model discloses low concentration residual peroxyacetic acid test paper has characteristics such as can detect 0.3ppm, check-out time 10 seconds, shelf life 18 months, but used peroxidase receives the influence of conditions such as temperature easily and inactivates or denaturalizes, and production raw materials cost is also relatively higher, and its detection that is applicable to peroxyacetic acid residual concentration, is not suitable for disinfection or the high concentration detection in industry.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide the test paper for rapidly determining the content of the hydrogen peroxide, which is simple to operate and wide in application range, and the preparation method thereof.
The purpose of the invention can be realized by the following technical scheme: the test paper for rapidly determining the hydrogen peroxide content comprises a test paper substrate and a test paper detection base layer, and is characterized in that a detection reactant is arranged on the test paper detection base layer, and the detection reactant comprises a color developing agent, a polymer, a catalyst and a pH regulator.
Further, the test paper detection base layer is prepared by soaking qualitative filter paper in a detection reactant solution and drying the solution, wherein the detection reactant solution contains the following components in percentage by mass: the concentration of the color developing agent is 5.0-10.0%, the concentration of the polymer is 0.3-1.0%, the concentration of the catalyst is 0.1-0.6%, and the pH value of the solution is adjusted to 5.5-6.5 by the pH adjusting agent.
Further, the color developing agent is potassium iodide.
Further, the polymer is selected from one or more of polyvinylpyrrolidone, polyvinyl alcohol and polyethylene glycol. Preferably, the polymer is a mixture of polyvinylpyrrolidone and polyvinyl alcohol in a mass ratio of 3.5:1, or a mixture of polyvinylpyrrolidone and polyethylene glycol in a mass ratio of 8: 1.
Further, the catalyst is a molybdate catalyst or a manganese salt catalyst. Wherein the molybdate catalyst is selected from one or more of sodium molybdate, ammonium molybdate or manganese molybdate; the manganese salt catalyst is manganese sulfate.
Further, the pH regulator is selected from commonly used buffers, such as at least one selected from potassium hydrogen phthalate-sodium hydroxide, potassium dihydrogen phosphate-dipotassium hydrogen phosphate, and citric acid-sodium citrate.
Furthermore, disodium ethylene diamine tetraacetate can be added into the detection reactant solution, and the addition amount of the disodium ethylene diamine tetraacetate is 0.01-0.1% of the mass percentage concentration of the disodium ethylene diamine tetraacetate in the detection reactant solution. The addition of the disodium ethylene diamine tetraacetate can reduce the interference of partial impurity ions in the sample, and further improve the detection accuracy.
A method for preparing test paper for quickly measuring hydrogen peroxide content includes mixing color developing agent, polymer, catalyst and pH regulator to prepare solution for detecting reactant, immersing qualitative filter paper in the solution for 1-5 min, taking out, baking in oven at 50-60 deg.C, cutting to size of 0.5cm to 0.6cm, and sticking it on substrate of test paper.
The test paper substrate can be made of a plastic sheet or a PET or PVC material, one end of the test paper substrate is a handheld area, the other end of the test paper substrate is a hydrogen peroxide detection area, and the test paper detection base layer is adhered to the hydrogen peroxide detection area.
In the invention, potassium iodide is used as a color developing agent, potassium iodide can be oxidized by hydrogen peroxide under the catalytic action of a catalyst to generate a yellowish-brown product, the color development can be more stable under the action of a specific polymer and molybdate or manganese salt, and the change of the color of a product and the content of hydrogen peroxide form a positive correlation relationship in a certain concentration range, so that the content of the detected substance hydrogen peroxide can be rapidly detected by comparing and reading the color of test paper and a standard color card.
The test paper using method comprises the following steps:
and immersing the test paper in the liquid to be tested, immediately taking out, absorbing residual liquid on the surface by using absorbent paper, horizontally placing the test paper upwards for 10 seconds, and comparing and reading with a color chart.
Compared with the prior art, the invention has the following beneficial effects:
1. the method changes the solution method into the test paper method, does not need specific environment and professional operation skills, can realize rapid detection and is convenient to use; the dosage of the test reagent is less than that of a laboratory method, so that the test cost is saved; the test paper substrate comprises a handheld area, so that an operator can take the test paper and avoid reagent pollution during testing, and the test paper substrate is widely applied to the fields of chemical engineering, spinning, environment, laboratories and the like.
2. Most of the existing hydrogen peroxide content detection test paper is used for detecting the residual concentration of hydrogen peroxide in the fields of food, daily chemicals, medical treatment and the like, and is not suitable for disinfection or industrial high-concentration detection. The invention adds polymer and potassium iodide to form loose compound, which keeps compound form in reaction process, to make the product in stable state, with stable color and easy storage.
3. The invention has reasonable production process, easily obtained used raw materials, lower equipment cost and capability of batch production.
Drawings
Fig. 1 is a schematic top view of the test strip of the present invention, wherein 1 is a detection substrate, 2 is a test strip substrate, and 3 is a handheld area.
Fig. 2 is a standard colorimetric card according to the present invention.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments.
Example 1
Preparing a mixed solution containing 10% of potassium iodide, 0.8% of polyvinylpyrrolidone and 0.3% of ammonium molybdate by using distilled water, adding a potassium hydrogen phthalate-sodium hydroxide solution to adjust the pH value to 6.0, completely immersing qualitative filter paper into the mixed solution, taking out the filter paper after 2 minutes, placing the filter paper in a 50 ℃ oven for about 20 minutes, cutting the dried test paper into strips of 0.5cm by 20cm, sticking the strips to one side of a PVC substrate, cutting the strips by taking the width of 0.5cm as a unit, and finally obtaining the test paper detection substrate with the size of 0.5cm by 0.5 cm. The structure is shown in fig. 1, and comprises a test paper substrate 2, a holding area 3 arranged on one side of the test paper substrate, and a test paper detection base layer 1 adhered on the other side of the test paper substrate.
In order to verify the detection effect of the test paper in example 1 on hydrogen peroxide with different concentrations, 100mg/L, 200mg/L, 400mg/L, 600mg/L, 800mg/L and 1000mg/L series of hydrogen peroxide standard solutions are prepared firstly, then the test paper in example 1 is immersed in the prepared solution to be detected, immediately taken out, the residual liquid on the surface is absorbed by absorbent paper, and the test paper is placed horizontally upwards for 10 seconds to observe the color development effect. Experiments show that the test paper has obvious color development gradation and complete color development immediately under the reaction of hydrogen peroxide with different concentrations, and the lower detection limit can reach an indication level of 100mg/L, as shown in figure 2. In order to verify the stability of the test paper in the air in example 1, the test paper prepared in example 1 is completely exposed in the air for 10 minutes, the appearance of the test paper is changed from white to yellowish, and then the test paper reacts with the series of hydrogen peroxide standard solutions, the color development effect can be still observed after 10 seconds, the color development level of the test paper is obvious, the test paper is completely developed immediately, and the lower detection limit can reach an indication level of 100 mg/L.
Example 2
Preparing a mixed solution containing 10% of potassium iodide, 0.7% of polyvinylpyrrolidone, 0.2% of polyvinyl alcohol and 0.3% of ammonium molybdate by using distilled water, adding a potassium hydrogen phthalate-sodium hydroxide solution to adjust the pH value to 6.0, completely immersing qualitative filter paper into the mixed solution, taking out the filter paper after 2 minutes, placing the filter paper in a 50 ℃ oven for about 20 minutes, cutting the dried test paper into strips of 0.5cm by 20cm, adhering the strips to one side of a PVC substrate, cutting the strips by taking the width of 0.5cm as a unit, and finally obtaining the test paper detection substrate with the size of 0.5cm by 0.5 cm.
In order to verify the detection effect of the embodiment 2 on hydrogen peroxide with different concentrations, 100mg/L, 200mg/L, 400mg/L, 600mg/L, 800mg/L and 1000mg/L series of hydrogen peroxide standard solutions are firstly prepared, then the test paper of the embodiment 2 is immersed into the prepared solution to be detected, immediately taken out, the residual liquid on the surface is absorbed by absorbent paper, and the color development effect is observed after the test paper is placed for 10 seconds horizontally upwards. Experiments show that the test paper has obvious color gradation in about 2 seconds and complete color development under the reaction of hydrogen peroxide with different concentrations, and the lower detection limit can reach the indication level of 100 mg/L. In order to verify the stability of the test paper in the air in the example 2, the test paper prepared in the example 2 is completely exposed in the air for 10 minutes, the appearance of the test paper still keeps white, and then the test paper reacts with the series of hydrogen peroxide standard solutions, the color development effect can be still observed after 2 seconds, the test paper has obvious color development gradation and complete color development immediately, and the lower detection limit can reach an indication level of 100 mg/L.
Example 3
Preparing a mixed solution containing 10% of potassium iodide, 0.7% of polyvinylpyrrolidone, 0.2% of polyvinyl alcohol, 0.05% of disodium ethylene diamine tetraacetate and 0.3% of ammonium molybdate by using distilled water, adding a potassium hydrogen phthalate-sodium hydroxide solution to adjust the pH value to 6.0, completely soaking qualitative filter paper into the mixed solution, taking out the filter paper after 2 minutes, placing the filter paper in a 50 ℃ oven for about 20 minutes, cutting the dried test paper into strips of 0.5cm and 20cm, adhering the strips to one side of a PVC substrate, cutting the strips by taking the width of 0.5cm as a unit, and finally obtaining the test paper detection substrate with the size of 0.5cm and 0.5 cm.
In order to verify the detection effect of the embodiment 3 on hydrogen peroxide with different concentrations, 100mg/L, 200mg/L, 400mg/L, 600mg/L, 800mg/L and 1000mg/L series of hydrogen peroxide standard solutions are firstly prepared, then the test paper of the embodiment 3 is immersed into the prepared solution to be detected, immediately taken out, the residual liquid on the surface is absorbed by absorbent paper, and the color development effect is observed after the test paper is placed for 10 seconds horizontally upwards. Experiments show that the test paper has obvious color gradation in about 2 seconds and complete color development under the reaction of hydrogen peroxide with different concentrations, and the lower detection limit can reach the indication level of 100 mg/L. To verify the stability of example 3 in air, the test paper obtained in example 3 was left to stand in air for 10 minutes after being completely exposed. The appearance of the test paper still keeps white, the test paper still can observe the color development effect after 2 seconds when reacting with the series of hydrogen peroxide standard solutions, the color development level of the test paper is obvious, the test paper can develop color completely immediately, and the lower detection limit can reach the indication level of 100 mg/L.
Example 4
Preparing a mixed solution containing 10% of potassium iodide, 0.8% of polyvinylpyrrolidone, 0.1% of polyethylene glycol, 0.05% of disodium ethylene diamine tetraacetate and 0.3% of ammonium molybdate by using distilled water, adding a potassium hydrogen phthalate-sodium hydroxide solution to adjust the pH value to 6.0, completely soaking qualitative filter paper into the mixed solution, taking out the filter paper after 2 minutes, placing the filter paper in a 50 ℃ oven for about 20 minutes, cutting the dried test paper into strips of 0.5cm and 20cm, adhering the strips to one side of a PVC substrate, cutting the strips by taking the width of 0.5cm as a unit, and finally obtaining the test paper detection substrate with the size of 0.5cm and 0.5 cm.
In order to verify the detection effect of the embodiment 4 on hydrogen peroxide with different concentrations, 100mg/L, 200mg/L, 400mg/L, 600mg/L, 800mg/L and 1000mg/L series of hydrogen peroxide standard solutions are firstly prepared, then the test paper of the embodiment 4 is immersed into the prepared solution to be detected, immediately taken out, the residual liquid on the surface is absorbed by absorbent paper, and the color development effect is observed after the test paper is placed for 10 seconds horizontally upwards. Experiments show that the test paper has obvious color gradation in about 10 seconds and complete color development under the reaction of hydrogen peroxide with different concentrations, and the lower detection limit can reach the indication level of 100 mg/L. To verify the stability of example 4 in air, the test paper obtained in example 4 was left to stand in air for 10 minutes after being completely exposed. The appearance of the test paper still keeps white, the test paper still can observe the color development effect after 10 seconds when reacting with the series of hydrogen peroxide standard solutions, the test paper has obvious color development gradation and complete color development immediately, and the lower detection limit can reach the indication level of 100 mg/L.
Example 5
Preparing a mixed solution containing 10% of potassium iodide, 0.8% of polyvinylpyrrolidone and 0.6% of manganese sulfate by using distilled water, adding a potassium dihydrogen phosphate-dipotassium hydrogen phosphate solution to adjust the pH value to 6.0, completely immersing qualitative filter paper into the mixed solution, taking out the filter paper after 2 minutes, placing the filter paper in a 50 ℃ oven for about 20 minutes, cutting the dried test paper into strips of 0.5cm and 20cm, sticking the strips to one side of a PVC (polyvinyl chloride) substrate, and cutting the strips by taking the width of 0.5cm as a unit to finally obtain a test paper detection base layer with the size of 0.5cm and 0.5 cm.
In order to verify the detection effect of the embodiment 5 on hydrogen peroxide with different concentrations, 100mg/L, 200mg/L, 400mg/L, 600mg/L, 800mg/L and 1000mg/L series of hydrogen peroxide standard solutions are firstly prepared, then the test paper of the embodiment 5 is immersed into the prepared solution to be detected, immediately taken out, the residual liquid on the surface is absorbed by absorbent paper, and the color development effect is observed after the test paper is placed for 10 seconds horizontally upwards. Experiments show that the test paper has obvious color development gradation under the reaction of hydrogen peroxide with different concentrations, the color development needs about 40 seconds to be complete, and the lower detection limit can reach the indication level of 100 mg/L. To verify the stability of example 5 in air, the test paper obtained in example 5 was left to stand in air for 10 minutes after being completely exposed. The appearance of the test paper is changed from white to yellowish, the test paper reacts with the series of hydrogen peroxide standard solutions, the color development effect can still be observed after 10 seconds, the color development level of the test paper is obvious, the test paper can develop color completely immediately, and the lower detection limit can reach 100mg/L indication level.
Example 6
Preparing a mixed solution containing 10% of potassium iodide, 0.7% of polyvinylpyrrolidone, 0.2% of polyvinyl alcohol and 0.6% of manganese sulfate by using distilled water, adding a potassium dihydrogen phosphate-dipotassium hydrogen phosphate solution to adjust the pH value to 6.0, completely immersing qualitative filter paper into the mixed solution for 2 minutes, taking out the filter paper, placing the filter paper in a 50 ℃ oven for about 20 minutes, cutting the dried test paper into strips of 0.5cm by 20cm, adhering the strips to one side of a PVC substrate, cutting the strips by taking the width of 0.5cm as a unit, and finally obtaining the test paper detection substrate with the size of 0.5cm by 0.5 cm.
In order to verify the detection effect of the test paper of example 6 on hydrogen peroxide with different concentrations, 100mg/L, 200mg/L, 400mg/L, 600mg/L, 800mg/L and 1000mg/L series of hydrogen peroxide standard solutions are prepared firstly, then the test paper of example 6 is immersed in the prepared solution to be detected, immediately taken out, the residual liquid on the surface is absorbed by absorbent paper, and the test paper is placed horizontally upwards for 10 seconds to observe the color development effect. Experiments show that the test paper has obvious color development gradation under the reaction of hydrogen peroxide with different concentrations, the color development is complete in about 40 seconds, and the lower detection limit can reach the indication level of 100 mg/L. To verify the stability of example 6 in air, the test strips of example 6 were left fully exposed to air for 10 minutes. The appearance of the test paper still keeps white, the test paper still can observe the color development effect after 10 seconds when reacting with the series of hydrogen peroxide standard solutions, the test paper has obvious color development gradation and complete color development immediately, and the lower detection limit can reach the indication level of 100 mg/L.
Example 7
Preparing a mixed solution containing 5% of potassium iodide, 0.3% of polyvinylpyrrolidone and 0.1% of manganese sulfate by using distilled water, adding a potassium dihydrogen phosphate-dipotassium hydrogen phosphate solution to adjust the pH value to 6.0, completely immersing qualitative filter paper into the mixed solution, taking out the filter paper after 2 minutes, placing the filter paper in a 60 ℃ oven for about 20 minutes, cutting the dried test paper into strips of 0.5cm and 20cm, sticking the strips to one side of a PVC (polyvinyl chloride) substrate, and cutting the strips by taking the width of 0.5cm as a unit to finally obtain a test paper detection base layer with the size of 0.5cm and 0.5 cm.
In order to verify the detection effect of the test paper in example 7 on hydrogen peroxide with different concentrations, 100mg/L, 200mg/L, 400mg/L, 600mg/L, 800mg/L and 1000mg/L series of hydrogen peroxide standard solutions are prepared firstly, then the test paper obtained in example 7 is immersed in the prepared solution to be detected, immediately taken out, the residual liquid on the surface is absorbed by absorbent paper, and the test paper is placed horizontally upwards for 10 seconds to observe the color development effect. Experiments show that the test paper has obvious color gradation in the reaction of hydrogen peroxide with different concentrations, has complete color development within about 30 seconds, and has a detection lower limit reaching an indication level of 100 mg/L. In order to verify the stability of the test paper of example 7 in the air, the test paper of example 7 is completely exposed in the air for 10 minutes, the appearance of the test paper still keeps white, and then the test paper reacts with the series of hydrogen peroxide standard solutions, the color development effect can be still observed after 10 seconds, the test paper has obvious color development gradation and is completely developed immediately, and the lower detection limit can reach an indication level of 100 mg/L.
The test paper obtained in the above examples 1 to 7 was stored in a sealed box at room temperature, and the concentration of hydrogen peroxide could be effectively detected after about 2 years.
In summary, the present invention is only a preferred embodiment, and not intended to limit the scope of the invention, and all equivalent changes and modifications in the shape, structure, characteristics and spirit of the present invention described in the claims should be included in the scope of the present invention.
Claims (10)
1. The test paper for rapidly determining the hydrogen peroxide content comprises a test paper substrate and a test paper detection base layer, and is characterized in that a detection reactant is arranged on the test paper detection base layer, and the detection reactant comprises a color developing agent, a polymer, a catalyst and a pH regulator.
2. The test paper for rapidly determining the content of hydrogen peroxide according to claim 1, wherein the test substrate of the test paper is prepared by soaking qualitative filter paper in a test reactant solution and then drying the soaked solution, and the test reactant solution contains the following components in percentage by mass: the concentration of the color developing agent is 5.0-10.0%, the concentration of the polymer is 0.3-1.0%, the concentration of the catalyst is 0.1-0.6%, and the pH value of the solution is adjusted to 5.5-6.5 by the pH adjusting agent.
3. The test paper for rapidly determining the content of hydrogen peroxide according to claim 1 or 2, wherein the color-developing agent is potassium iodide.
4. The test paper for rapidly determining the content of hydrogen peroxide according to claim 1 or 2, wherein the polymer is one or more selected from polyvinylpyrrolidone, polyvinyl alcohol and polyethylene glycol.
5. The test paper for rapidly determining hydrogen peroxide content according to claim 4, wherein the polymer is a mixture of polyvinylpyrrolidone and polyvinyl alcohol, or a mixture of polyvinylpyrrolidone and polyethylene glycol.
6. The reagent strip for rapid determination of hydrogen peroxide according to claim 1 or 2, wherein the catalyst is a molybdate catalyst or a manganese catalyst.
7. The reagent strip for rapid determination of hydrogen peroxide according to claim 6, wherein the molybdate catalyst is selected from one or more of sodium molybdate, ammonium molybdate or manganese molybdate; the manganese salt catalyst is manganese sulfate.
8. The test paper for rapidly determining the content of hydrogen peroxide according to claim 1 or 2, wherein the pH regulator is at least one selected from the group consisting of potassium hydrogen phthalate-sodium hydroxide, potassium dihydrogen phosphate-dipotassium hydrogen phosphate, and citric acid-sodium citrate.
9. The reagent paper for rapid determination of hydrogen peroxide content of claim 2, wherein the reagent solution for detection is further added with disodium edetate.
10. A method for preparing a test paper for rapidly determining hydrogen peroxide content as claimed in claim 1 or 2, wherein the color developing agent, the polymer, the catalyst and the pH adjusting agent are mixed to prepare a solution for detecting the reactant, the qualitative filter paper is soaked in the solution for 1 to 5 minutes, then the solution is taken out, dried in an oven at 50 to 60 ℃, cut and stuck on a test paper substrate.
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| CN114280040A (en) * | 2021-11-18 | 2022-04-05 | 中国农业科学院油料作物研究所 | Test strip for detecting peroxide value of edible oil, preparation method and application thereof |
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