CN109142254A - A kind of multiwave length spectro photometric detection method based on Fenton oxidation ABTS chromogenic assay content of hydrogen peroxide - Google Patents
A kind of multiwave length spectro photometric detection method based on Fenton oxidation ABTS chromogenic assay content of hydrogen peroxide Download PDFInfo
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Abstract
The invention discloses a kind of multiwave length spectro photometric detection methods based on Fenton oxidation ABTS chromogenic assay content of hydrogen peroxide, include the following steps that (1) establishes standard curve and its equation of linear regression；(2) content of hydrogen peroxide in sample is measured.Multiwavelength spectrophotometry of the invention for indicator, can quickly detect content of hydrogen peroxide in solution using ferrous ion as activator, with 2 '-hydrazines-bis- -3- ethyl benzo thiazole phenanthroline -6- sulfonic acid (ABTS).The present invention does not need expensive instrument and equipment and reagent, it only needs a common ultraviolet-uisible spectrophotometer that can meet measurement to require, reduce cost of determination, it can be widely used in environmental protection, daily use chemicals and medical industry, quick, simple, accurate detection carried out to content of hydrogen peroxide in the various samples through oxidation, bleaching or disinfection.
Description
Technical field
The invention belongs to hydrogen peroxide detection technique fields, and in particular to one kind is based on Fenton oxidation ABTS chromogenic assay mistake
Aoxidize the multiwave length spectro photometric detection method of hydrogen content.
Background technique
Due to having the characteristics that stronger oxidability and environmentally protective, hydrogen peroxide is in environmental protection, daily use chemicals, food, doctor
In treatment and dyeing, it is widely used as oxidant, disinfectant, bactericidal agent and bleaching agent.In addition, hydrogen peroxide may be used also
There is stronger oxidability to be generated by activation such as transition metal ions, metal oxide, microwave radiation and ultraviolet radiations
Hydroxyl radical free radical (E0=1.8-2.7V), it is widely used in environment protection field, is especially removed in water treatment field difficult to degrade organic
Pollutant and inactivation pathogenic microorganisms.Using in hydrogen peroxide process, often along with the decomposition of oxidant and residual
It is remaining.Human body is made to accelerate aging it is reported that hydrogen peroxide remaining in human body can consume antioxidant；Long Term Contact is eaten by mistake
The residual hydrogen peroxide of low concentration will lead to human genetic material damage and gene mutation, or even induce cancer.Therefore, in mistake
In the use process of hydrogen oxide, need to monitor the variation of hydrogen peroxide residual amount.
Currently, the measuring method of content of hydrogen peroxide mainly have titration, chemoluminescence method, chromatography, spectrophotometry,
Test paper colorimetric method, amperometry etc..Mainly there are iodimetric titration and permanganimetric method, this method high sensitivity, measurement in titration
As a result accurate, but there is also the deficiencies of complicated for operation, disturbing factor is more.Chromatography is with specificity is strong, accuracy is high, anti-interference
The advantages that ability is strong, such as patent ZL 201310745895.7, however chromatography needs to cause to measure using expensive chromatograph
It is with high costs.Chemoluminescence method has many advantages, such as that high sensitivity, detection limit are low, measurement result is accurate, but when equally existing measurement
Between the long, shortcomings such as cost of determination is high.UV-VIS spectrophotometry measurement result is reliable, finding speed is fast, operation is opposite
Simplicity, such as patent ZL 201310745891.9 and ZL 201410034574.0, but, reaction reagent longer there is also the reaction time
In place of toxic the deficiencies of easily causing environmental pollution, test paper colorimetric method has many advantages, such as that easy to operate, finding speed is fast, such as patent ZL
201310337923.1, but there is also test paper preparation costs it is higher, measurement result accuracy is poor, sensitivity is lower the deficiencies of it
Place.Amperometry measurement result is accurate and reliable, high sensitivity, such as patent ZL200780034519.X, but equally exists survey
Determine the deficiencies of achievement is high, measurement required time is long, poor anti jamming capability place.
Summary of the invention
It is an object of the invention to overcome prior art defect, provide a kind of based on Fenton oxidation ABTS chromogenic assay peroxide
Change the multiwave length spectro photometric detection method of hydrogen content.
Technical scheme is as follows:
A kind of multiwave length spectro photometric detection method based on Fenton oxidation ABTS chromogenic assay content of hydrogen peroxide, including
Following steps:
(1) standard curve and its equation of linear regression are established: ferrous ions soln and ABTS solution being added to acid pure
The first mixed solution is obtained in water purification, then measures absorbance value of first mixed solution at specific absorption wavelength, and school zero；Point
The Hydrogen peroxide standard sample that various concentration is added not into above-mentioned first mixed solution forms the second mixed solution, makes hydrogen peroxide
Concentration gradient is formed in the second mixed solution；Second mixed solution reacts 0.4-2.6 minutes in 4~36 DEG C of progress oxidative color-developings
Afterwards, absorbance value of second mixed solution at above-mentioned specific absorption wavelength after measurement colour developing, is denoted as a；Using a as abscissa,
Using ultimate density of the hydrogen peroxide in the second mixed solution as ordinate, standard curve and its equation of linear regression are established；
Wherein, ferrous ions soln concentration is 1.8-41mmol L-1, ABTS solution concentration is 0.8-22mmol L-1, acid pure water
PH value be 1.8-3.1, the volume ratio of ferrous ions soln, ABTS solution and acid pure water is 0.02-0.5: 0.1-2.1:
1:
(2) it measures content of hydrogen peroxide in sample: hydrogen peroxide sample to be measured being added into above-mentioned first mixed solution
Third mixed solution is formed, after 4~36 DEG C of progress oxidative color-developings react 0.4-2.6 minutes, third mixing is molten after measurement colour developing
Absorbance value of the liquid at above-mentioned specific absorption wavelength, is denoted as b；B is updated to the linear of gained standard curve in step (1) to return
Return in equation, then hydrogen peroxide to be measured is calculated by the volume relationship of hydrogen peroxide sample to be measured and third mixed solution
The content of hydrogen peroxide in sample；Wherein, in third mixed solution, the volume of the hydrogen oxide sample to be measured and acid pure water
Than being 0.04-0.97: 1；
Above-mentioned specific absorption wavelength is at least one of 415nm, 650nm, 732nm and 820nm.
In a preferred embodiment of the invention, the solute in the ferrous ions soln is ferrous perchlorate, chlorine
Change at least one of ferrous iron, ferrous nitrate, ferrous sulfate and ferric sulfate.
In a preferred embodiment of the invention, the concentration gradient in the step (1) includes 0,5,10,20,30
And 40 μm of ol L-1。
In a preferred embodiment of the invention, the ferrous ions soln concentration is 2-40mmol L-1, described
ABTS solution concentration is 1-20mmol L-1, the pH value of the acidity pure water is 2-3.
In a preferred embodiment of the invention, in first mixed solution, ferrous ions soln, ABTS solution
Volume ratio with acid pure water is 0.025-0.5: 0.1-2: 1.
In a preferred embodiment of the invention, in the third mixed solution, the hydrogen oxide sample to be measured with
The volume ratio of acid pure water is 0.05-0.95: 1.
In a preferred embodiment of the invention, the temperature of oxidative color-developing reaction is 5~35 DEG C, and the time is
0.5-2.5 minutes.
The beneficial effects of the present invention are:
1, Multiwavelength spectrophotometry of the invention using ferrous ion as activator, with 2 '-hydrazines-bis- -3- ethyl benzo
Thiazoline -6- sulfonic acid (ABTS) is indicator, can quickly detect content of hydrogen peroxide in solution.
2, the present invention generates hydroxyl using the Fenton's reaction of ferrous ion activated hydrogen peroxide compared with existing measuring method
Free radical quickly aoxidize 2 '-hydrazines-bis- -3- ethyl benzo thiazole phenanthroline -6- sulfonic acid (ABTS) generate green and stable ABTS from
Content of hydrogen peroxide is measured by base, i.e., in the reaction solution containing ferrous ion, hydrogen peroxide and ABTS, ferrous ion with
Hydrogen peroxide passes through Fenton's reaction first and quickly generates hydroxyl radical free radical, then the hydroxyl radical free radical fast reaction of ABTS and generation
The ABTS free radical of green, then the absorbance value of foundation ABTS free radical are generated to measure content of hydrogen peroxide.Ferrous ion is living
The hydroxyl radical free radical oxidation-reduction potential for changing the Fenton's reaction generation of hydrogen peroxide is very high, up to 1.8-2.7V, oxidability
It is very strong, quantitative chromogenic quickly can occur with ABTS and react, to achieve the purpose that quickly to measure content of hydrogen peroxide.
3, the invention is simple and feasible, does not need additionally to carry out ultraviolet irradiation, microwave irradiation, electron beam irradiation, ultrasound sky again
Change, aeration, additional electromagnetic field and heating；Compared with traditional iodimetric titration, minute of the present invention was foreshortened to by 30-45 minutes and is only needed
0.5-2.5 minutes；Compared with permanganimetric method, the present invention has that easy to operate, result is reliable, by other ions and has
Machine object influences small feature；Compared with chemoluminescence method, the medicament that the present invention uses inexpensively easily is obtained, minute is short；With color
Spectrometry is compared, and the present invention is relatively inexpensive using instrument；Compared with the UV-VIS spectrophotometry that azo-based dyestuff fades,
The present invention has the advantages that environmentally protective, to add in continuous mode Fe2+, ABTS and its product be environment amenable chemistry
Substance；With using catalase-N, N- diethyl p-phenylenediamine's test paper colorimetric method is compared, and the present invention has experimental drug honest and clean
The features such as valence, easy to maintain, easy configuration, easy acquisition, high sensitivity；Compared with amperometry, the present invention has easy to operate, survey
Try the features such as time is short, testing cost is low.
4, the present invention does not need expensive instrument and equipment and reagent, it is only necessary to a common ultraviolet-uisible spectrophotometer
Meet measurement to require, reduce cost of determination, can be widely used for environmental protection, in daily use chemicals and medical industry, is aoxidized, bleached to various
Or content of hydrogen peroxide carries out quick, simple, accurate detection in the sample of disinfection.
Detailed description of the invention
Fig. 1 is the canonical plotting of the embodiment of the present invention 1；
Fig. 2 is the canonical plotting of the embodiment of the present invention 2；
Fig. 3 is the canonical plotting of the embodiment of the present invention 3；
Fig. 4 is the canonical plotting of the embodiment of the present invention 4.
Specific embodiment
Technical solution of the present invention is further explained and described below by way of specific embodiment combination attached drawing.
In following embodiments, room temperature is 4~36 DEG C, preferably 5~35 DEG C of
Embodiment 1:
A kind of Multiwavelength spectrophotometry based on Fenton oxidation ABTS chromogenic assay content of hydrogen peroxide of the present embodiment,
Include the following steps:
Step (1): production standard curve:
1, by the 2 '-hydrazines of the ferrous sulfate solution of 0.25mL and 0.5mL-bis- -3- ethyl benzo thiazole phenanthroline -6- sulfonic acid
(ABTS) sulfate mother liquor is added into the acid pure water of 1.5mL, obtains the first mixed solution, then measures the first mixed solution
Absorbance value at 415nrn, and school zero；
2, the Hydrogen peroxide standard solution that 0.25mL various concentration gradient is added into the first mixed solution respectively forms the
Two mixed solutions, total reaction volume 2.5mL；
3, after normal-temperature reaction 1min, absorbance value of second mixed solution at 415nm after measurement reaction is denoted as b；
4, using b as abscissa, using ultimate density of the hydrogen peroxide in the second mixed solution as ordinate, mark is established
Directrix curve and its equation of linear regression；
Illustrate:
Wherein, ferrous sulfate solution concentration is 10mmol L-1, ABTS sulfate mother liquid concentration is 10mmol L-1, acid
Pure water pH is 2.4；Ferrous sulfate solution, ABTS solution, acid pure water and the second mixed solution volume ratio be 0.1:
0.2:0.68: 1；The various concentration gradient refer to concentration of the hydrogen peroxide in the second mixed solution be respectively 0,5,10,
20,30 and 40 μm of ol L-1。
Step (2): content of hydrogen peroxide in measurement sample:
1, the ABTS mother liquor of the ferrous sulfate solution of 0.25mL and 0.5mL is added into the acid pure water of 1.7mL, is obtained
Then first mixed solution measures absorbance value of first mixed solution at 415nm, and school zero；
2, the known concentration of hydrogen peroxide that 0.05mL is added into the first mixed solution is 1mol L-1Water sample, obtain
Three mixed solutions, after normal-temperature reaction 1min, absorbance value of the third mixed solution at 415nm after measurement colour developing is denoted as c；
3, c value is updated in step (1) in the equation of linear regression of gained standard curve, peroxide in water sample is calculated
Change the content of hydrogen.
The resulting standard curve of the present embodiment is as shown in Figure 1, gained linear regression formula is as follows:
[hydrogen peroxide]Reaction=A415÷0.0419
Therefore, concentration of hydrogen peroxide calculation formula is as follows in water sample:
[hydrogen peroxide]Sample=VAlways÷VSample× [hydrogen peroxide] is anti-It answers
=2.5 0.05 × (A of ÷415÷0.0419)
The A that the present embodiment measures415It is 0.867, concentration of hydrogen peroxide in water sample, which is calculated, in substitution above-mentioned formula is
1034.6μmol L-1, measurement deviation 3.5% illustrates that this measuring method is accurate and reliable.
The present embodiment generates hydroxyl radical free radical using ferrous sulfate activated hydrogen peroxide and quickly aoxidizes the generation of ABTS sulfate
Green ABTS free radical, method is simple and easy, does not need additionally to carry out ultraviolet irradiation, microwave irradiation, electron beam irradiation, ultrasound again
Cavitation, aeration, additional electromagnetic field and heating, minute is short, and measurement result error is small, ferrous sulfate, the ABTS sulfuric acid added
Salt and their reaction product are environment amenable chemical substance, do not need expensive instrument and equipment and reagent, it is only necessary to one
Common ultraviolet-uisible spectrophotometer can meet measurement and require, and reduce costs.Measuring method can be widely used for environmental protection, print
In dye and medical industry, content of hydrogen peroxide in the various samples through oxidation, bleaching or disinfection is carried out quick, environmentally friendly, effective
Detection.
Embodiment 2
A kind of Multiwavelength spectrophotometry based on Fenton oxidation ABTS chromogenic assay content of hydrogen peroxide of the present embodiment,
Include the following steps:
Step (1): production standard curve:
1, by the ABTS sulfate mother liquor of the ferrous sulfate solution of 0.25mL and 0.5mL be added to 1.5mL acidity it is pure
In water, the first mixed solution is obtained, then measures absorbance value of first mixed solution at 650nm, and school zero；
2, the Hydrogen peroxide standard solution that 0.25mL various concentration gradient is added into the first mixed solution respectively forms the
Two mixed solutions, total reaction volume 2.5mL；
3, after normal-temperature reaction 0.5min, absorbance value of second mixed solution at 650nm after measurement reaction is denoted as b；
4, using b as abscissa, using ultimate density of the hydrogen peroxide in the second mixed solution as ordinate, mark is established
Directrix curve and its equation of linear regression；
Illustrate:
Wherein, ferrous sulfate solution concentration is 25mmol L-1, ABTS sulfate mother liquid concentration is 10mmol L-1, acid
Pure water pH is 2.5；Ferrous sulfate solution, ABTS sulfate liquor, acid pure water and the second mixed solution volume ratio be
0.1:0.2:0.6:1；The various concentration gradient refer to concentration of the hydrogen peroxide in the second mixed solution be respectively 0,5,
10,20,30 and 40 μm of ol L-1。
Step (2): content of hydrogen peroxide in measurement sample:
1, by the ABTS sulfate mother liquor of the ferrous sulfate solution of 0.25mL and 0.5mL be added to 1.5mL acidity it is pure
In water, the first mixed solution is obtained, then measures absorbance value of first mixed solution at 650nm, and school zero；
2, the known concentration of hydrogen peroxide that 0.25mL is added into the first mixed solution is 0.1mmol L-1Water sample, obtain
Third mixed solution, after normal-temperature reaction 1min, absorbance value of the third mixed solution at 650nm after measurement colour developing is denoted as c；
3, c value is updated in step (1) in the equation of linear regression of gained standard curve, peroxide in water sample is calculated
Change the content of hydrogen.
The resulting standard curve of the present embodiment is as shown in Fig. 2, gained linear regression formula is as follows:
[hydrogen peroxide]Reaction=A650÷0.01728
Therefore, concentration of hydrogen peroxide calculation formula is as follows in water sample:
[hydrogen peroxide]Sample=VAlways÷VSample× [hydrogen peroxide] is anti-It answers
=2.5 0.25 × (A of ÷650÷0.01728)
The A that the present embodiment measures650It is 0.175, concentration of hydrogen peroxide in water sample, which is calculated, in substitution above-mentioned formula is
101.3μmol L-1, measurement deviation 1.3% illustrates that this measuring method is accurate and reliable.
Embodiment 3
A kind of Multiwavelength spectrophotometry based on Fenton oxidation ABTS chromogenic assay content of hydrogen peroxide of the present embodiment,
Include the following steps:
Step (1): production standard curve:
1, by the ABTS sulfate mother liquor of the ferrous sulfate solution of 0.25mL and 0.5mL be added to 1.7mL acidity it is pure
In water, the first mixed solution is obtained, then measures absorbance value of first mixed solution at 732nm, and school zero；
2, the Hydrogen peroxide standard solution that 0.05mL various concentration gradient is added into the first mixed solution respectively forms the
Two mixed solutions, total reaction volume 2.5mL；
3, after normal-temperature reaction 1min, absorbance value of second mixed solution at 732nm after measurement reaction is denoted as b；
4, using b as abscissa, using ultimate density of the hydrogen peroxide in the second mixed solution as ordinate, mark is established
Directrix curve and its equation of linear regression；
Illustrate:
Wherein, ferrous sulfate solution concentration is 10mmol L-1, ABTS sulfate mother liquid concentration is 10mmol L-1, acid
Pure water pH is 2.0；Ferrous sulfate solution, ABTS solution, acid pure water and the second mixed solution volume ratio be 0.1: 0.2
:0.68:1；The various concentration gradient refer to concentration of the hydrogen peroxide in the second mixed solution be respectively 0,5,10,20,
30 and 40 μm of ol L-1。
Step (2): content of hydrogen peroxide in measurement sample:
1, by the 2 '-hydrazines of the ferrous sulfate solution of 0.25mL and 0.5mL-bis- -3- ethyl benzo thiazole phenanthroline -6- sulfonic acid sulphur
Hydrochlorate mother liquor is added into the acid pure water of 1.7mL, obtains the first mixed solution, then measures the first mixed solution in 732nm
The absorbance value at place, and school zero；
2, the known concentration of hydrogen peroxide that 0.05mL is added into the first mixed solution is 1mmol L-1Water sample, obtain
Three mixed solutions, after normal-temperature reaction 1min, absorbance value of the third mixed solution at 732nm after measurement colour developing is denoted as c；
3, c value is updated in step (1) in the equation of linear regression of gained standard curve, peroxide in water sample is calculated
Change the content of hydrogen.
The resulting standard curve of the present embodiment is as shown in figure 3, gained linear regression formula is as follows:
[hydrogen peroxide]Reaction=A732÷0.02178
Therefore, concentration of hydrogen peroxide calculation formula is as follows in water sample:
[hydrogen peroxide]Sample=VAlways÷VSample× [hydrogen peroxide]Reaction
=2.5 0.05 × (A of ÷732÷0.02178)
The A that the present embodiment measures732It is 0.429, concentration of hydrogen peroxide in water sample, which is calculated, in substitution above-mentioned formula is
984.8μmol L-1, measurement deviation is -1.5%, illustrates that this measuring method is accurate and reliable.
Embodiment 4
A kind of Multiwavelength spectrophotometry based on Fenton oxidation ABTS chromogenic assay content of hydrogen peroxide of the present embodiment,
Include the following steps:
Step (1): production standard curve:
1, by the ABTS sulfate mother liquor of the ferrous sulfate solution of 0.25mL and 0.5mL be added to 1.5mL acidity it is pure
In water, the first mixed solution is obtained, then measures absorbance value of first mixed solution at 820nm, and school zero；
2, the Hydrogen peroxide standard solution that 0.25mL various concentration gradient is added into the first mixed solution respectively forms the
Two mixed solutions, total reaction volume 2.5mL；
3, after normal-temperature reaction 0.5min, absorbance value of second mixed solution at 820nm after measurement reaction is denoted as b；
4, using b as abscissa, using ultimate density of the hydrogen peroxide in the second mixed solution as ordinate, mark is established
Directrix curve and its equation of linear regression；
Illustrate:
Wherein, ferrous sulfate solution concentration is 25mmol L-1, ABTS sulfate mother liquid concentration is 10mmol L-1, acid
Pure water pH is 2.5；Ferrous sulfate solution, ABTS sulfate liquor, acid pure water and the second mixed solution volume ratio be
0.1:0.2:0.6:1；The various concentration gradient refer to concentration of the hydrogen peroxide in the second mixed solution be respectively 0,5,
10,20,30 and 40 μm of ol L-1。
Step (2): content of hydrogen peroxide in measurement sample:
1, by the ABTS acid sulfate mother liquor of the ferrous sulfate solution of 0.25mL and 0.5mL be added to 1.5mL acidity it is pure
In water purification, the first mixed solution is obtained, then measures absorbance value of first mixed solution at 820nm, and school zero；
2, the known concentration of hydrogen peroxide that 0.25mL is added into the first mixed solution is 0.1mmol L-1Water sample, obtain
Third mixed solution, after normal-temperature reaction 1min, absorbance value of the third mixed solution at 820nm after measurement colour developing is denoted as c；
3, c value is updated in step (1) in the equation of linear regression of gained standard curve, peroxide in water sample is calculated
Change the content of hydrogen.
The resulting standard curve of the present embodiment is as shown in figure 4, gained linear regression formula is as follows:
[hydrogen peroxide]Reaction=A820÷0.01965
Therefore, concentration of hydrogen peroxide calculation formula is as follows in water sample:
[hydrogen peroxide]Sample=VAlways÷VSample× [hydrogen peroxide]Reaction
=2.5 0.25 × (A of ÷820÷0.01965)
The A that the present embodiment measures820It is 0.205, concentration of hydrogen peroxide in water sample, which is calculated, in substitution above-mentioned formula is
104.3μmol L-1, measurement deviation 4.3% illustrates that this measuring method is accurate and reliable.
In the various embodiments described above, it is described acidity pure water be one of distilled water, deionized water, pure water or in which
Several combinations by arbitrary volume ratio.Cuvette material used is glass or quartz, cell length 1cm, 2cm, 3cm, 5cm
Or 10cm.
The foregoing is only a preferred embodiment of the present invention, the range that the present invention that therefore, it cannot be limited according to is implemented, i.e.,
Equivalent changes and modifications made in accordance with the scope of the invention and the contents of the specification should still be within the scope of the present invention.
Claims (7)
1. a kind of multiwave length spectro photometric detection method based on Fenton oxidation ABTS chromogenic assay content of hydrogen peroxide, feature
It is: includes the following steps:
(1) standard curve and its equation of linear regression are established: ferrous ions soln and ABTS solution are added to acid pure water
In the first mixed solution, then measure absorbance value of first mixed solution at specific absorption wavelength, and school zero；Respectively to
The Hydrogen peroxide standard sample that various concentration is added in above-mentioned first mixed solution forms the second mixed solution, makes hydrogen peroxide the
Concentration gradient is formed in two mixed solutions；Second mixed solution after 4~36 DEG C of progress oxidative color-developings react 0.4-2.6 minutes,
Absorbance value of second mixed solution at above-mentioned specific absorption wavelength after measurement colour developing, is denoted as a；Using a as abscissa, with mistake
Ultimate density of the hydrogen oxide in the second mixed solution establishes standard curve and its equation of linear regression as ordinate；Wherein,
Ferrous ions soln concentration is 1.8-41mmol L-1, ABTS solution concentration is 0.8-22mmol L-1, the pH value of acid pure water
For 1.8-3.1, the volume ratio of ferrous ions soln, ABTS solution and acid pure water is 0.02-0.5: 0.1-2.1: 1；
(2) it measures content of hydrogen peroxide in sample: hydrogen peroxide sample to be measured being added into above-mentioned first mixed solution and is formed
Third mixed solution, after 4~36 DEG C of progress oxidative color-developings react 0.4-2.6 minutes, third mixed solution exists after measurement colour developing
Absorbance value at above-mentioned specific absorption wavelength, is denoted as b；B is updated to the linear regression side of gained standard curve in step (1)
Cheng Zhong, then hydrogen peroxide sample to be measured is calculated by the volume relationship of hydrogen peroxide sample to be measured and third mixed solution
The content of middle hydrogen peroxide；Wherein, in third mixed solution, the volume ratio of the hydrogen oxide sample to be measured and acid pure water is
0.04-0.97:1；
Above-mentioned specific absorption wavelength is at least one of 415nm, 650nm, 732am and 820nm.
2. multiwave length spectro photometric detection method as described in claim 1, it is characterised in that: in the ferrous ions soln
Solute is at least one of ferrous perchlorate, frerrous chloride, ferrous nitrate, ferrous sulfate and ferric sulfate.
3. multiwave length spectro photometric detection method as described in claim 1, it is characterised in that: the concentration in the step (1)
Gradient includes 0,5,10,20,30 and 40 μm of ol L-1。
4. multiwave length spectro photometric detection method as described in claim 1, it is characterised in that: the ferrous ions soln concentration
For 2-40mmol L-1, the ABTS solution concentration is 1-20mmol L-1, the pH value of the acidity pure water is 2-3.
5. multiwave length spectro photometric detection method as described in claim 1, it is characterised in that: in first mixed solution,
The volume ratio of ferrous ions soln, ABTS solution and acid pure water is 0.025-0.5: 0.1-2: 1.
6. multiwave length spectro photometric detection method as described in claim 1, it is characterised in that: in the third mixed solution,
The volume ratio of the hydrogen oxide sample to be measured and acid pure water is 0.05-0.95: 1.
7. multiwave length spectro photometric detection method as described in claim 1, it is characterised in that: the temperature of the oxidative color-developing reaction
Degree is 5~35 DEG C, and the time is 0.5-2.5 minutes.
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Cited By (4)
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|---|---|---|---|---|
| CN110567901A (en) * | 2019-10-14 | 2019-12-13 | 广西民族大学 | detection method and detection reagent for hydrogen peroxide in food |
| CN113149177A (en) * | 2021-05-26 | 2021-07-23 | 华侨大学 | Method for quickly removing indole in water |
| CN113281331A (en) * | 2021-05-17 | 2021-08-20 | 福州大学 | Machine learning-based rapid detection method for copper ions in water environment |
| CN114965327A (en) * | 2020-11-30 | 2022-08-30 | 广东轻工职业技术学院 | Rapid detection method and kit for glucose and application of kit |
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| CN110567901A (en) * | 2019-10-14 | 2019-12-13 | 广西民族大学 | detection method and detection reagent for hydrogen peroxide in food |
| CN114965327A (en) * | 2020-11-30 | 2022-08-30 | 广东轻工职业技术学院 | Rapid detection method and kit for glucose and application of kit |
| CN113281331A (en) * | 2021-05-17 | 2021-08-20 | 福州大学 | Machine learning-based rapid detection method for copper ions in water environment |
| CN113149177A (en) * | 2021-05-26 | 2021-07-23 | 华侨大学 | Method for quickly removing indole in water |
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