CN108982491B - Test paper for rapidly detecting concentration of peroxyacetic acid and application thereof - Google Patents
Test paper for rapidly detecting concentration of peroxyacetic acid and application thereof Download PDFInfo
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Abstract
The invention discloses test paper for rapidly detecting concentration of peroxyacetic acid and application thereof. Belongs to the field of chemical analysis. The preparation method of the test paper comprises three parts of mother liquor preparation, filter paper pretreatment and test paper preparation. The test paper can be used for detecting the concentration of the peroxyacetic acid disinfectant on site, when the concentration of the peroxyacetic acid reaches the effective sterilization concentration (0.18%), the surface of the test paper is yellow, the higher the concentration is, the deeper the yellow is, the change is obvious, and the existence of hydrogen peroxide does not have obvious interference phenomenon on the yellowing process of the test paper. The method does not need large equipment or accessories, can greatly save cost, and has simple test process, reliable result, convenience and rapidness.
Description
Technical Field
The invention belongs to the field of chemical analysis, and particularly relates to test paper for rapidly detecting peroxyacetic acid concentration and application of the test paper in detecting effective concentration of peroxyacetic acid disinfectant.
Background
The peracetic acid disinfectant is used as a broad-spectrum, high-efficiency and novel environment-friendly disinfectant, the concentration of the disinfectant can be reduced after repeated use, and the sterilizing capability can be greatly reduced when the concentration is lower than 0.18%. In order to ensure the sterilization effect, the sterilization effect needs to be periodically detected during application. The existing methods for detecting peroxyacetic acid mainly comprise a spectrophotometric method, a fluorescence method, an infrared method and the like. However, these methods have the disadvantages of complicated operation, expensive instrument requirement, etc., and are not suitable for rapid field detection.
Rapid methods for detecting peroxyacetic acid concentrations have entered the field of researchers, such as: (1) chinese patent CN87106131.7 (peroxyacetic acid test paper and its preparation method), (2) chinese patent CN201310336589.8 (a rapid determination method of peroxyacetic acid residue), and (3) chinese patent CN201220408884.0 (test paper for detecting low concentration residual peroxyacetic acid). However, they all have certain limitations, such as patent (1) cannot exclude the interference of a large amount of hydrogen peroxide in the disinfectant, and patents (2) and (3) can only be used for detecting the residual concentration of peroxyacetic acid, and cannot detect the effective concentration of peroxyacetic acid. Therefore, the development of a convenient and reliable product for detecting the effective concentration of peracetic acid is necessary.
Disclosure of Invention
In order to solve the problems in the prior art, the invention firstly aims to provide a test paper for rapidly detecting the real-time concentration of peroxyacetic acid, wherein the test paper can show color changes of different degrees when used for detecting peroxyacetic acid disinfectant with different concentrations, and the concentration data of peroxyacetic acid can be read by comparing with a standard colorimetric card.
The invention also aims to provide a method for detecting the effective concentration of the peroxyacetic acid disinfectant by using the test paper.
The technical scheme adopted by the invention is as follows:
a test paper for rapidly detecting the concentration of peroxyacetic acid is prepared by the following steps: (1) preparing a mother solution: dissolving a reaction substrate, a pH buffering agent and a dispersing agent by using deionized water, and filtering by using quick qualitative filter paper after ultrasonic oscillation for 5min to obtain a mother solution with uniform color; (2) pretreatment of peroxyacetic acid determination test paper: respectively soaking the medium-speed qualitative filter paper in absolute ethyl alcohol and deionized water for 30min, taking out, drying, and cutting to the required size for later use; (3) preparation of test paper for determining peroxyacetic acid: putting the filter paper treated in the step (2) into the mother liquor, soaking for 5 minutes, taking out, putting on a horizontal glass plate, drying at 30 ℃ in vacuum, pasting on a PVC bottom plate to obtain a product, and sealing and storing; the reaction substrate is a mixture of water-soluble tetramethyl benzidine hydrochloride or tetramethyl benzidine sulfate and ferrous ions (ferrous sulfate or ferrous chloride and the like), preferably a mixture of tetramethyl benzidine hydrochloride and ferrous sulfate, wherein the concentration of the tetramethyl benzidine hydrochloride is 0.1-0.5 g/L, and the concentration of the ferrous sulfate is 10-20 g/L;
the pH buffering agent is a mixture of disodium hydrogen phosphate and sodium dihydrogen phosphate or a mixture of dipotassium hydrogen phosphate and potassium dihydrogen phosphate, preferably a mixture of potassium phosphate salts, wherein the concentration of the potassium dihydrogen phosphate in the mixed solution is 5.0-10.0 g/L, and the concentration of the dipotassium hydrogen phosphate is 15-35 g/L;
the dispersing agent is polyethylene glycol, and the concentration is 4-15 g/L.
Preferably, the test paper pretreated in the step (2) is cut into a size of 5-6mm × 5-6mm, and the size of the PVC base plate in the step (3) is 6-8mm × 50-80 mm.
In a second aspect, a method for detecting peroxyacetic acid concentration by using the test paper is provided, which comprises the following steps: and (3) immersing the peroxyacetic acid determination test paper into a sample to be tested, taking out, comparing the color developed by the test paper with a standard colorimetric card after the color development is complete, and reading out the concentration data of the peroxyacetic acid.
Preferably, the standard closed color card is prepared by the following method: diluting with deionized water to obtain peroxyacetic acid standard solutions with the concentrations of 0.06%, 0.12%, 0.18%, 0.24% and 0.30%, immersing the prepared peroxyacetic acid test paper in the standard solution, taking out after 1s, throwing away excessive liquid, reacting for 3min, comparing the color of the test paper with a Pantong color chart to find out a corresponding standard color, wherein the test paper is light yellow green when the concentration is lower than 0.18%, and the surface of the test paper is yellow when the concentration reaches 0.18%, and the higher the concentration is, the darker the yellow is, and the color change is obvious; and obtaining a standard color comparison card according to the standard color.
In a third aspect, the application of the test paper in detecting the effective concentration of the peroxyacetic acid disinfectant is provided.
The principle utilized by the invention is as follows:
the peracetic acid has strong oxidizability, can oxidize TMB hydrochloride and ferrous ions, and when the concentration of the peracetic acid is low, the peracetic acid reacts with the TMB hydrochloride to generate a blue substance, and when the concentration of the peracetic acid is high, on one hand, the ferrous ions can be oxidized into ferric ions, so that the test paper shows yellow, and on the other hand, the pH of the solution is relatively low, and under the condition of low pH, the blue substance generated by the reaction of the peracetic acid and the TMB is easily converted into yellow diphenoquinone, so that the test paper becomes yellow.
The invention has the following advantages:
(1) the method can be used for detecting the concentration of the peroxyacetic acid disinfectant on site, and when the concentration is lower than 0.18%, the test paper is light yellow green, which indicates that the solution can not meet the sterilization requirement; when the concentration reaches 0.18%, the surface of the test paper is yellow, which indicates that the solution has a good sterilization effect, and the higher the concentration is, the darker the yellow is, and the color change is obvious;
(2) the invention can eliminate the interference of hydrogen peroxide, and is superior to other methods;
(3) the invention does not need other equipment accessories, can greatly save the cost, and has simple operation, convenience and rapidness.
Drawings
Fig. 1 is a view of a standard colorimetric card for on-site measurement of concentration of peroxyacetic acid disinfectant.
Detailed Description
The features and advantages of the present invention will be further understood from the following detailed description taken in conjunction with the accompanying drawings. The examples provided are merely illustrative of the method of the present invention and do not limit the remainder of the disclosure in any way.
The test strips used in the following examples were pretreated: and respectively soaking the medium-speed qualitative filter paper in absolute ethyl alcohol and deionized water for a period of time, and taking out and drying.
Example 1
Weighing 0.01g of TMB hydrochloride, dissolving in deionized water, and fixing the volume to 100mL in a volumetric flask to obtain a solution A for later use; weighing 0.38g of monopotassium phosphate and 1.45g of dipotassium phosphate in the same beaker, weighing 0.78g of ferrous sulfate and 0.2g of polyethylene glycol in another beaker, dissolving with a small amount of solution A respectively, mixing, fixing the volume to 50mL with the solution A, pouring the mixed solution with the fixed volume into the beaker, carrying out ultrasonic treatment for 5min, and filtering with quick qualitative filter paper to obtain a filtrate, namely a mother solution; cutting the pretreated test paper into the size of 5mm multiplied by 5mm, completely soaking the test paper in mother liquor for 5min, taking out the test paper, drying the test paper in vacuum at the temperature of 30 ℃, and adhering the test paper to one end of a strip-shaped PVC bottom plate with the size of 6mm multiplied by 50mm to prepare the peroxyacetic acid detection test paper.
And immersing the prepared test paper into a solution to be tested, taking out after 1s, spin-drying the redundant liquid, and reading the concentration of the peroxyacetic acid according to the color after 3 min. As shown in fig. 1, from left to right, the standard colorimetric card is prepared by detecting the color development effects of the test paper with the concentrations of 0.06%, 0.12%, 0.18%, 0.24% and 0.30%. At concentrations of 0.06% and 0.12%, the test paper was pale green, while at concentrations of 0.18%, the test paper turned yellow, the higher the concentration the darker the yellow.
The peracetic acid test paper prepared in the embodiment 1 is used for testing a certain brand of peracetic acid disinfectant, a part of the disinfectant is placed in a beaker, the test paper strip is inserted into the disinfectant for 1s and then taken out, excess liquid is thrown off, after 3min, the test paper strip turns yellow, and the concentration of the test paper strip is higher than 0.30% when being found by comparing with a standard colorimetric card, which indicates that the peracetic acid disinfectant reaches the effective concentration and can be used for sterilization. The brand disinfectant is placed in a beaker, the beaker is placed for 10 days at normal temperature in an open mode, the disinfectant is detected by using the test strip in the embodiment 1, the test strip is taken out after being soaked for 1s, and the test strip is observed to be light blue after 3min, which indicates that the concentration of the test strip is lower than 0.12 percent, namely, the peracetic acid in the open disinfectant does not reach the effective concentration and can not be used for sterilization.
Example 2
Weighing 0.02g of TMB hydrochloride, dissolving in deionized water, and fixing the volume to 100mL in a volumetric flask to obtain a solution A for later use; weighing 0.5g of monopotassium phosphate and 1.78g of dipotassium phosphate in the same beaker, weighing 0.85g of ferrous sulfate and 0.4g of polyethylene glycol in another beaker, dissolving with a small amount of solution A respectively, mixing, fixing the volume to 50mL with the solution A, pouring the mixed solution with the fixed volume into the beaker, carrying out ultrasonic treatment for 5min, and filtering to obtain a filtrate, namely a mother solution; cutting the pretreated test paper into the size of 6mm multiplied by 6mm, completely soaking the test paper in mother liquor for 8min, taking out the test paper, drying the test paper in vacuum at the temperature of 30 ℃, and adhering the test paper to one end of a strip-shaped PVC bottom plate with the size of 8mm multiplied by 80mm to prepare the peroxyacetic acid detection test paper.
Example 3
Weighing 0.02g of TMB hydrochloride, dissolving in deionized water, and fixing the volume to 100mL in a volumetric flask to obtain a solution A for later use; weighing 0.35g of sodium dihydrogen phosphate and 1.28g of disodium hydrogen phosphate in the same beaker, weighing 0.95g of ferrous chloride and 0.5g of polyethylene glycol in another beaker, dissolving with a small amount of solution A respectively, mixing, fixing the volume to 50mL with the solution A, pouring the mixed solution with the fixed volume into the beaker, performing ultrasonic treatment for 5min, and filtering to obtain a filtrate, namely a mother solution; cutting the pretreated test paper into the size of 5mm multiplied by 6mm, completely soaking the test paper in mother liquor for 10min, taking out the test paper, drying the test paper in vacuum at the temperature of 30 ℃, and adhering the test paper to one end of a strip-shaped PVC bottom plate with the size of 6mm multiplied by 70mm to prepare the peroxyacetic acid detection test paper.
Claims (4)
1. The test paper for rapidly detecting the concentration of peroxyacetic acid is characterized by comprising the following steps: (1) preparing a mother solution: dissolving a reaction substrate, a pH buffering agent and a dispersing agent by using deionized water, and filtering by using quick qualitative filter paper after ultrasonic oscillation for 5min to obtain a mother solution with uniform color; (2) pretreatment of peroxyacetic acid determination test paper: respectively soaking the medium-speed qualitative filter paper in absolute ethyl alcohol and deionized water for 30min, taking out, drying, and cutting to the required size for later use; (3) preparation of test paper for determining peroxyacetic acid: putting the filter paper treated in the step (2) into the mother liquor, soaking for 5 minutes, taking out, putting on a horizontal glass plate, drying at 30 ℃ in vacuum, pasting on a PVC bottom plate to obtain a product, and sealing and storing;
the reaction substrate is a mixture of water-soluble tetramethyl benzidine hydrochloride and ferrous sulfate, wherein the concentration of the tetramethyl benzidine hydrochloride is 0.1-0.5 g/L, and the concentration of the ferrous sulfate is 10-20 g/L; the pH buffering agent is a mixture of dipotassium hydrogen phosphate and potassium dihydrogen phosphate, the concentration of the potassium dihydrogen phosphate in the mixed solution is 5.0-10.0 g/L, and the concentration of the dipotassium hydrogen phosphate is 15-35 g/L; the dispersing agent is polyethylene glycol, and the concentration of the polyethylene glycol is 4-15 g/L.
2. The strip for rapidly detecting concentration of peroxyacetic acid as claimed in claim 1, wherein the strip pretreated in step (2) is cut into a size of 5-6mm x 5-6mm, and the PVC substrate in step (3) has a size of 6-8mm x 50-80 mm.
3. A method of detecting peroxyacetic acid concentration using the test strip of claim 1 or 2, comprising the steps of: soaking the peroxyacetic acid determination test paper into a sample to be tested, taking out the peroxyacetic acid determination test paper, comparing the color developed by the test paper with a standard colorimetric card after the peroxyacetic acid determination test paper is completely developed, and reading out the concentration data of the peroxyacetic acid;
the standard colorimetric card is prepared by the following method: diluting with deionized water to obtain peroxyacetic acid standard solutions with the concentrations of 0.06%, 0.12%, 0.18%, 0.24% and 0.30%, immersing the prepared peroxyacetic acid test paper in the standard solution, taking out after 1s, throwing away excessive liquid, reacting for 3min, comparing the color of the test paper with a Pantong color chart to find out a corresponding standard color, wherein the test paper is light yellow green when the concentration is lower than 0.18%, and the surface of the test paper is yellow when the concentration reaches 0.18%, and the higher the concentration is, the darker the yellow is, and the color change is obvious; and obtaining a standard color comparison card according to the standard color.
4. Use of a test strip according to claim 1 or 2 for detecting the effective concentration of a peroxyacetic acid disinfectant.
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| CN109991218A (en) * | 2019-03-29 | 2019-07-09 | 迪瑞医疗科技股份有限公司 | A kind of preparation method of coagulase Test paper |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6087089A (en) * | 1997-11-12 | 2000-07-11 | Integrated Biomedical Technology, Inc. | Peroxide and chlorine test strip |
| WO2018017809A1 (en) * | 2016-07-21 | 2018-01-25 | Lonza Inc. | Method and kit for determining peracetic acid concentration in disinfectant solutions |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1012531B (en) * | 1987-10-14 | 1991-05-01 | 冯光鉴 | Test paper for determining peroxy-acetic acid and its prepn. method |
| US7651860B2 (en) * | 2004-12-17 | 2010-01-26 | Enviro Tech Chemical Services, Inc. | Method of analyzing low levels of peroxyacetic acid in water |
| CN202945249U (en) * | 2012-08-17 | 2013-05-22 | 昆山爱达斯工业设计有限公司 | Low-concentration residual peroxyacetic acid detection test paper |
| CN103439322B (en) * | 2013-08-05 | 2015-12-09 | 广东环凯微生物科技有限公司 | The rapid assay methods that a kind of Peracetic acid is residual |
| CN106568770A (en) * | 2016-11-11 | 2017-04-19 | 上海利康消毒高科技有限公司 | Preparation method of rapid test card for rapidly testing peroxyacetic acid concentration |
| CN106841181A (en) * | 2016-12-27 | 2017-06-13 | 山东新华医疗器械股份有限公司 | A kind of peracetic acid disinfectant quickly determines test paper and preparation method |
| CN108107039B (en) * | 2017-11-27 | 2020-04-14 | 广东环凯微生物科技有限公司 | Peroxyacetic acid determination test paper and determination method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6087089A (en) * | 1997-11-12 | 2000-07-11 | Integrated Biomedical Technology, Inc. | Peroxide and chlorine test strip |
| WO2018017809A1 (en) * | 2016-07-21 | 2018-01-25 | Lonza Inc. | Method and kit for determining peracetic acid concentration in disinfectant solutions |
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Effective date of registration: 20210805 Address after: Area 4, Huangma Industrial Park, Qinbei District, Qinzhou City, Guangxi Zhuang Autonomous Region Patentee after: GUANGXI QINGRONG HEALTH DISINFECTION PRODUCT Co.,Ltd. Address before: 430072 Hubei Province, Wuhan city Wuchang District of Wuhan University Luojiashan Patentee before: WUHAN University |