CN106841181A - A kind of peracetic acid disinfectant quickly determines test paper and preparation method - Google Patents
A kind of peracetic acid disinfectant quickly determines test paper and preparation method Download PDFInfo
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- CN106841181A CN106841181A CN201611227585.6A CN201611227585A CN106841181A CN 106841181 A CN106841181 A CN 106841181A CN 201611227585 A CN201611227585 A CN 201611227585A CN 106841181 A CN106841181 A CN 106841181A
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- test paper
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- acid disinfectant
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
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Abstract
A kind of peracetic acid disinfectant quickly determines test paper and preparation method, belongs to disinfecting medicine field.It is characterized in that：By the solution treatment containing KI and stabilizer, the mass ratio of sodium thiosulfate, sodium carbonate and polyvinyl alcohol is 1 to test paper in solution：0.5~5：0.25~2.5.Test paper is completely submerged in upper mother liquor, after immersion 3min ~ 5min, test paper is taken out and is dried at 50 DEG C ~ 70 DEG C, treat that test paper is completely dried, cut into suitable size and obtain final product；This method is easy to operate, as a result clear and intuitive, is entirely capable of meeting the clinical requirement to peracetic acid disinfectant concentration precision, and does not have any special technical requirements to operator, and Disinfection Effect that can be intuitively rapidly to peracetic acid disinfectant used is estimated.
Description
Technical field
A kind of peracetic acid disinfectant quickly determines test paper and preparation method, belongs to disinfecting medicine field.
Background technology
Peracetic acid disinfectant is increasingly taken seriously in terms of Medical instrument sterilization sterilizing, but it goes out in storage and sterilization
During bacterium, it may occur that decompose or constantly consumed, when its concentration is less than certain limit value, in equal process time,
Disinfection Effect is difficult to be guaranteed, therefore, medical institutions need the concentration for often determining peracetic acid disinfectant to disappear to ensure it
Toxic effect is really.The method of current national standard detection peracetic acid soln concentration is iodimetric titration, in addition with other detection method bags
Include colorimetric method, fluorescence method, electrochemical process, AAS and chromatography.The process of iodometric determination Peracetic acid content is cumbersome,
Go out that result is slower, other method is required to special equipment and reagent, and high cost, complex operation is not easy to practical application.And adopt
Determining test paper with peracetic acid disinfectant of the invention directly can rapidly determine the concentration of peracetic acid disinfectant, so as to it
Disinfection Effect makes objective evaluation.
The content of the invention
The technical problem to be solved in the present invention is：Overcome the deficiencies in the prior art, there is provided a kind of easy to use, quick, knot
Fruit accurately peracetic acid disinfectant directly perceived quickly determines test paper and preparation method.
The technical solution adopted for the present invention to solve the technical problems is：The peracetic acid disinfectant quickly determines test paper,
It is characterized in that：By the solution treatment containing KI and stabilizer, the mass concentration of KI is test paper in solution：5%~
15%。
The present invention provides a kind of measure test paper for determining peroxyacetic acid concentration in peracetic acid disinfectant.Used by the present invention
KI can show different face with Peracetic acid reaction generation elemental iodine, the elemental iodine of various concentrations under sour environment
Color.This determine test paper need to only soak when using take out in solution to be determined after can quickly determine dense with standard color comparison card contrast
Degree, without carrying out cumbersome titration isoconcentration test, with the accurate advantage of easy to use, quick, visual result.
Preferably, the mass concentration of KI is in described solution：8%~10%.
Described stabilizer is sodium thiosulfate, sodium carbonate and polyvinyl alcohol, sodium thiosulfate, sodium carbonate and polyvinyl alcohol
Mass ratio be 1：0.5~5：0.25~2.5.Inventor adds specific stabilizer on the basis of liquor kalii iodide, and adjusts
Ratio between them, is obtained mother liquor, then processes test paper, by increasing capacitance it is possible to increase the colour developing aberration between adjacent concentration, extension test paper
Effective storage life, accelerates colour developing speed.
Sodium thiosulfate mass concentration in the solution is：0.1%~1%；Sodium carbonate mass concentration in the solution is
0.1%~1%；Polyvinyl alcohol mass concentration in the solution is：0.05%~0.5%.
Preferably, described sodium carbonate mass concentration in the solution is 0.4% ~ 0.6%；Described sodium thiosulfate is molten
Mass concentration in liquid is：0.4%~0.6%；Described polyvinyl alcohol mass concentration in the solution is：0.3%~0.4%.
By the content of each reagent in above-mentioned preferred control and regulation mother liquor, can be obtained to concentration sensitive widest range
Measure test paper, the chromatic aberration between various concentrations substantially, easily distinguishes.
Preferably, described test paper is qualitative filter paper, quantitative filter paper or quantitative chromatography filter paper.Mother liquor formula of the invention is right
Different test paper has good adaptability, and above-mentioned preferred test paper type is conducive to more preferably colour developing faster.
A kind of above-mentioned peracetic acid disinfectant quickly determines the preparation method of test paper, it is characterised in that preparation process is：
1）KI, sodium thiosulfate, sodium carbonate and polyvinyl alcohol are configured to the aqueous solution, are stirred as mother liquor；
2）Test paper is completely submerged in mother liquor, after immersion 3min ~ 5min, test paper is taken out and is dried at 50 DEG C ~ 70 DEG C, wait to try
Paper is completely dried, and cuts into suitable size and obtains final product；
3）The test paper color of the peracetic acid disinfectant normal concentration tested according to made measure test paper is made standard color card.
The test paper that determines of the invention can intuitively determine rapidly the concentration of peracetic acid disinfectant, it is ensured that thimerosal disappears
Really, KI can show toxic effect with Peracetic acid reaction generation elemental iodine, the elemental iodine of various concentrations under sour environment
Different colors, inventor selects KI and stabilizer in the proper ratio and concentration, and mother liquor is obtained, and then processes test paper,
Drying, then shear forming, are fixed on the ground not absorbed water by suitable method, you can obtain peracetic acid disinfectant survey
Test paper is determined, while making standard color card.The content of each reagent, can be obtained to various concentrations model in precise control and regulation mother liquor
Enclose the Series Measurement test paper of sensitivity, the concentration of the peracetic acid disinfectant for determining various concentrations scope.During test, only need by
Determine test paper and put into peracetic acid disinfectant solution to be measured and uniformly soak rapidly, 10s ~ 15s is that observable is surveyed after taking-up
Determine the color change of test paper, the change according to color compares to determine the concentration of surveyed peracetic acid disinfectant with standard color card.This
Method is easy to operate, as a result clear and intuitive, is entirely capable of meeting the clinical requirement to peracetic acid disinfectant concentration precision, and right
Operator does not have any special technical requirements, and Disinfection Effect that can be intuitively rapidly to peracetic acid disinfectant used is commented
Estimate.
Preferably, step 2）Described in test paper in mother liquor soak time be 3.5min ~ 4min.Preferred soak time energy
The test paper of better quality is prepared, colour developing is faster.
Preferably, step 2）Described in test paper drying temperature be 55 DEG C ~ 60 DEG C, drying time be 20 min ~ 24min.
Preferred immersion and drying time can prepare effective time longer measure test paper.
Preferably, step 3）It is middle test peracetic acid disinfectant normal concentration method be：Test paper immersion will be determined different
Taken out after 1s in the peracetic acid disinfectant of normal concentration, by after 10s ~ 15s, determining each color for determining test paper to be made phase
Answer the standard color card of color.
Compared with prior art, a kind of peracetic acid disinfectant of the invention quickly determines test paper and preparation method and is had
Beneficial effect be：The present invention provides a kind of measure test paper for determining peroxyacetic acid concentration in peracetic acid disinfectant and preparation side
Method.KI used by the present invention can be with Peracetic acid reaction generation elemental iodine, and the elemental iodine of various concentrations is in sour environment
Under show different colors.Inventor adds specific stabilizer on the basis of liquor kalii iodide, and adjusts between them
Ratio, be obtained mother liquor, then process test paper, shear forming after drying.This is determined when test paper is used need to only soak to be determined
Concentration can be quickly determined with standard color comparison card contrast after being taken out in solution, it is accurate with easy to use, quick, visual result
Advantage.The test paper that determines of the invention can intuitively determine rapidly the concentration of peracetic acid disinfectant, it is ensured that thimerosal disappears
Toxic effect is really.Inventor selects KI and stabilizer in the proper ratio and concentration, and mother liquor is obtained, and then processes test paper, through drying
It is dry, then shear forming, it is fixed on the ground not absorbed water by suitable method, you can obtain peracetic acid disinfectant and determine examination
Paper, while making standard color card.The content of each reagent, can be obtained quick to various concentrations scope in precise control and regulation mother liquor
The Series Measurement test paper of sense, the concentration of the peracetic acid disinfectant for determining various concentrations scope, and to out concentration sensitive
The most obvious mother liquor proportioning of widest range, adjacent concentration aberration.This method is easy to operate, as a result clear and intuitive, is entirely capable of meeting
The clinical requirement to peracetic acid disinfectant concentration precision, and there is no any special technical requirements to operator, can be directly perceived
Rapidly the Disinfection Effect to peracetic acid disinfectant used is estimated.
Specific embodiment
With reference to specific embodiment, the present invention will be further described, and wherein embodiment 1 is most preferred embodiment.
Embodiment 1
1）By KI 9.00g, sodium thiosulfate 0.5g, natrium carbonicum calcinatum 0.5g, polyvinyl alcohol 0.35g is water-soluble using purifying
Added after solution to 100ml as mother liquor；
2）Test paper is completely submerged in mother liquor, after immersion 4min, test paper is taken out and is dried at 57 DEG C, drying time is
22min, treats that test paper is completely dried, and cuts into suitable size and obtains final product；
3）Taken out after 1s in the peracetic acid disinfectant that test paper immersion various criterion concentration will be determined, by after 10s ~ 15s, determining
The color of test paper is respectively determined to be made the standard color card of respective color.
Embodiment 2
By KI 8.00g, sodium thiosulfate 0.6g, natrium carbonicum calcinatum 0.3g, polyvinyl alcohol 0.15g, using purifying water dissolves
After add to 100ml as mother liquor；
2）Test paper is completely submerged in mother liquor, after immersion 4min, test paper is taken out and is dried at 55 DEG C, drying time is
22min, treats that test paper is completely dried, and cuts into suitable size and obtains final product；
3）Taken out after 1s in the peracetic acid disinfectant that test paper immersion various criterion concentration will be determined, by after 10s ~ 15s, determining
The color of test paper is respectively determined to be made the standard color card of respective color.
Embodiment 3
By KI 10.00g, sodium thiosulfate 0.1g, natrium carbonicum calcinatum 0.5g, polyvinyl alcohol 0.25g is water-soluble using purifying
Added after solution to 100ml as mother liquor；
2）Test paper is completely submerged in mother liquor, after immersion 3.5min, test paper is taken out and is dried at 60 DEG C, drying time is 20
Min, treats that test paper is completely dried, and cuts into suitable size and obtains final product；
3）Taken out after 1s in the peracetic acid disinfectant that test paper immersion various criterion concentration will be determined, by after 10s ~ 15s, determining
The color of test paper is respectively determined to be made the standard color card of respective color.
Embodiment 4
By KI 5g, sodium thiosulfate 0.1g, natrium carbonicum calcinatum 1g, polyvinyl alcohol 0.5g, using being added after purifying water dissolves
To 100ml as mother liquor；
2）Test paper is completely submerged in mother liquor, after immersion 3min, test paper is taken out and is dried at 50 DEG C, drying time is
30min, treats that test paper is completely dried, and cuts into suitable size and obtains final product；
3）Taken out after 1s in the peracetic acid disinfectant that test paper immersion various criterion concentration will be determined, by after 10s ~ 15s, determining
The color of test paper is respectively determined to be made the standard color card of respective color.
Embodiment 5
By KI 15.00g, sodium thiosulfate 1g, natrium carbonicum calcinatum 0.1g, polyvinyl alcohol 0.05g, after water dissolves are purified
Add to 100ml as mother liquor；
2）Test paper is completely submerged in mother liquor, after immersion 5min, test paper is taken out and is dried at 70 DEG C, drying time is
15min, treats that test paper is completely dried, and cuts into suitable size and obtains final product；
3）Taken out after 1s in the peracetic acid disinfectant that test paper immersion various criterion concentration will be determined, by after 10s ~ 15s, determining
The color of test paper is respectively determined to be made the standard color card of respective color.
Comparative example 1
1）By KI 9.00g, add after water dissolves to 100ml as mother liquor by the use of being purified；
2）Test paper is completely submerged in mother liquor, after immersion 4min, test paper is taken out and is dried at 57 DEG C, drying time is
22min, treats that test paper is completely dried, and cuts into suitable size and obtains final product；
3）Taken out after 1s in the peracetic acid disinfectant that test paper immersion various criterion concentration will be determined, by after 10s ~ 15s, determining
The color of test paper is respectively determined to be made the standard color card of respective color.
Comparative example 2
1）By KI 9.00g, sodium thiosulfate 5g, natrium carbonicum calcinatum 0.05g, polyvinyl alcohol 1g, after water dissolves are purified
Add to 100ml as mother liquor；
2）Test paper is completely submerged in mother liquor, after immersion 4min, test paper is taken out and is dried at 57 DEG C, drying time is
22min, treats that test paper is completely dried, and cuts into suitable size and obtains final product；
3）Taken out after 1s in the peracetic acid disinfectant that test paper immersion various criterion concentration will be determined, by after 10s ~ 15s, determining
The color of test paper is respectively determined to be made the standard color card of respective color.
The performance test results of the embodiment of table 1 and comparative example
。
The concentration range determined in table 1 is the scope that this test paper can judge concentration by standard color comparison card, and aberration is institute
Value of chromatism between the adjacent concentration of standard color comparison card processed, is determined by color difference meter.Each embodiment of the term of validity can accurately develop the color
Effective time.
The above, is only presently preferred embodiments of the present invention, is not the limitation for making other forms to the present invention, is appointed
What those skilled in the art changed possibly also with the technology contents of the disclosure above or be modified as equivalent variations etc.
Effect embodiment.But it is every without departing from technical solution of the present invention content, according to technical spirit of the invention to above example institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection domain of technical solution of the present invention.
Claims (10)
1. a kind of peracetic acid disinfectant quickly determines test paper, it is characterised in that：Test paper is by molten containing KI and stabilizer
Liquid treatment, the mass concentration of KI is in solution：5%~15%.
2. a kind of peracetic acid disinfectant according to claim 1 quickly determines test paper, it is characterised in that：Described solution
The mass concentration of middle KI is：8%~10%.
3. a kind of peracetic acid disinfectant according to claim 1 quickly determines test paper, it is characterised in that：Described stabilization
Agent is sodium thiosulfate, sodium carbonate and polyvinyl alcohol, and the mass ratio of sodium thiosulfate, sodium carbonate and polyvinyl alcohol is 1：0.5~5：
0.25~2.5。
4. a kind of peracetic acid disinfectant according to claim 3 quickly determines test paper, it is characterised in that：The thio sulphur
Sour sodium mass concentration in the solution is：0.1%~1%；Sodium carbonate mass concentration in the solution is 0.1% ~ 1%；Polyvinyl alcohol exists
Mass concentration in solution is：0.05%~0.5%.
5. a kind of peracetic acid disinfectant according to claim 4 quickly determines test paper, it is characterised in that：Described carbonic acid
Sodium mass concentration in the solution is 0.4% ~ 0.6%；Described sodium thiosulfate mass concentration in the solution is：0.4%~
0.6%；Described polyvinyl alcohol mass concentration in the solution is：0.3%~0.4%.
6. a kind of peracetic acid disinfectant according to claim 1 quickly determines test paper, it is characterised in that：Described test paper
It is qualitative filter paper, quantitative filter paper or quantitative chromatography filter paper.
7. the peracetic acid disinfectant described in a kind of any one of claim 1 ~ 6 quickly determines the preparation method of test paper, its feature
It is that preparation process is：
1）KI, sodium thiosulfate, sodium carbonate and polyvinyl alcohol are configured to the aqueous solution, are stirred as mother liquor；
2）Test paper is completely submerged in mother liquor, after immersion 3min ~ 5min, test paper is taken out and is dried at 50 DEG C ~ 70 DEG C, wait to try
Paper is completely dried, and cuts into suitable size and obtains final product；
3）The test paper color of the peracetic acid disinfectant normal concentration tested according to made measure test paper is made standard color card.
8. a kind of peracetic acid disinfectant according to claim 7 quickly determines the preparation method of test paper, it is characterised in that：
Step 2）Described in test paper in mother liquor soak time be 3.5min ~ 4min.
9. a kind of peracetic acid disinfectant according to claim 7 quickly determines the preparation method of test paper, it is characterised in that：
Step 2）Described in test paper drying temperature be 55 DEG C ~ 60 DEG C, drying time be 20 min ~ 24min.
10. a kind of peracetic acid disinfectant according to claim 7 quickly determines the preparation method of test paper, and its feature exists
In：Step 3）It is middle test peracetic acid disinfectant normal concentration method be：The mistake of test paper immersion various criterion concentration will be determined
Taken out after 1s in fluoroacetic acid thimerosal, by after 10s ~ 15s, determining each color for determining test paper to be made the standard of respective color
Colour atla.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108107039A (en) * | 2017-11-27 | 2018-06-01 | 广东环凯微生物科技有限公司 | A kind of test paper for determining peroxy-acetic acid and its assay method |
| CN108827955A (en) * | 2018-07-16 | 2018-11-16 | 浙江大学 | A method of detection peracetic acid soln concentration |
| CN108982491A (en) * | 2018-07-24 | 2018-12-11 | 武汉大学 | A kind of test paper of quick detection peroxyacetic acid concentration and application |
| CN110538339A (en) * | 2019-09-11 | 2019-12-06 | 衡水诺盾生物科技有限公司 | hydrogen peroxide plasma five-class card sterilization challenge device and manufacturing method thereof |
| CN110927155A (en) * | 2019-12-25 | 2020-03-27 | 上海三爱思试剂有限公司 | Dibromo hydantoin disinfectant detection test paper and preparation method and application thereof |
| CN114152663A (en) * | 2021-01-08 | 2022-03-08 | 郑州笨农农业科技有限公司 | Disinfectant concentration measuring device and method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108107039A (en) * | 2017-11-27 | 2018-06-01 | 广东环凯微生物科技有限公司 | A kind of test paper for determining peroxy-acetic acid and its assay method |
| CN108107039B (en) * | 2017-11-27 | 2020-04-14 | 广东环凯微生物科技有限公司 | Peroxyacetic acid determination test paper and determination method thereof |
| CN108827955A (en) * | 2018-07-16 | 2018-11-16 | 浙江大学 | A method of detection peracetic acid soln concentration |
| CN108982491A (en) * | 2018-07-24 | 2018-12-11 | 武汉大学 | A kind of test paper of quick detection peroxyacetic acid concentration and application |
| CN110538339A (en) * | 2019-09-11 | 2019-12-06 | 衡水诺盾生物科技有限公司 | hydrogen peroxide plasma five-class card sterilization challenge device and manufacturing method thereof |
| CN110927155A (en) * | 2019-12-25 | 2020-03-27 | 上海三爱思试剂有限公司 | Dibromo hydantoin disinfectant detection test paper and preparation method and application thereof |
| CN114152663A (en) * | 2021-01-08 | 2022-03-08 | 郑州笨农农业科技有限公司 | Disinfectant concentration measuring device and method |
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Application publication date: 20170613 |