CN105606604A - Rapid hydrogen peroxide test paper and preparation method thereof - Google Patents
Rapid hydrogen peroxide test paper and preparation method thereof Download PDFInfo
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- CN105606604A CN105606604A CN201610010329.5A CN201610010329A CN105606604A CN 105606604 A CN105606604 A CN 105606604A CN 201610010329 A CN201610010329 A CN 201610010329A CN 105606604 A CN105606604 A CN 105606604A
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Abstract
The invention discloses rapid hydrogen peroxide test paper and a preparation method thereof. The test paper comprises a base plate and a test paper piece; one end of the base plate serves as a handheld end, the other end of the base plate serves as a test paper piece paste end, the test paper piece is pasted at the test paper piece paste end, and the test paper piece is soaked in a test paper soaking solution and then dried to obtain the test paper; the test paper soaking solution is prepared by mixing an antioxidant system, an enzyme protection system and a color developing agent, the antioxidant system is prepared by dissolving tea polyphenol, vitamin c and sodium thiosulfate into water, and the enzyme protection system is prepared by dissolving sucrose, polyethylene glycol and bovine serum albumin into water. Accordingly, groups, prone to oxidization, of tetramethyl benzidine are protected by the antioxidant system, so that the rapid hydrogen peroxide test paper can be preserved for 1 year at normal temperature.
Description
Technical field
The present invention relates to a kind of hydrogen peroxide quick detection test paper and preparation method thereof, belong to chemical rapid detection technical field.
Background technology
Hydrogen peroxide is being commonly called as of hydrogenperoxide steam generator, is divided into food stage and technical grade, and it is many that industrial hydrogen peroxide contains arsenic, heavy metal etc.Plant noxious material. The food that part businessman need to brighten at some is as the production of water-soaked food, tinned fruit, Flour product etc.The industrial hydrogen peroxide of violated use high concentration in journey, to improve the outward appearance of product. " National Standard of the People's Republic of China's food additiveAdd agent use sanitary standard " in clearly regulation, in processed food process, the use amount of hydrogen peroxide should not exceed prohibitive amount.The U.S. and other countries have also formulated respectively the use standard of peroxide value in different food products. The detection of hydrogen peroxide in food at presentMethod has chemical titration analysis method, and chemical titration analysis method comprises potassium permanganate standard titration, indirect iodimetric titration and cerium sulfate method.But these method complex operations are consuming time longer, react insensitive. Therefore, in food inspection, set up one simply, accuratelyDetection method is very important.
There is at present the Test paper for the hydrogen peroxide fast detecting in food, compared with traditional precision instrument, there is economy, economizeTime, the feature such as easy. Whether can help domestic consumer to understand hydrogen peroxide in own edible food has residual. Quick at presentIn the research in Test paper field, most of scientific research personnel's the activity of all relatively closing the horseradish peroxidase of mentioning in injecting methodWith and stability, and do not have a kind of method clearly to propose developer (tetramethyl benzidine) stability in quick detection test paperResearch, therefore limited hydrogen peroxide test paper at low temperatures (2-8 DEG C) preserve.
Summary of the invention
The object of the present invention is to provide a kind of hydrogen peroxide quick detection test paper and preparation method thereof, it has used composite antioxidant,Protect easy oxidized group in tetramethyl benzidine, hydrogen peroxide quick detection test paper can be preserved 1 year at normal temperatures.
For achieving the above object, the present invention has adopted following technical proposals. A kind of hydrogen peroxide quick detection test paper, comprise substrate,Test-paper, one end of substrate is handheld terminal, and the other end is test-paper paste-side, and test-paper is pasted test-paper on paste-side, test paperSheet, after test paper soak soaks, is dried and obtains; Described test paper soak is mixed by antioxidant system, enzyme protection system and developerClose and form, described antioxidant system is dissolved in water and is obtained by Tea Polyphenols, vitamin c, sodium thiosulfate.
Further preferably, described enzyme protection system is dissolved in water and is obtained by sucrose, polyethylene glycol, bovine serum albumin(BSA).
More specifically, described antioxidant system is by 0.5-1.5g Tea Polyphenols, 0.1-0.3g vitamin c, 1.0-2.0g thiosulfuric acidSodium is dissolved in 100ml water and obtains.
More specifically, described enzyme protection system is pure by 2.0-3.5g sucrose, 0.5-1.5g polyethylene glycol, 0.5-1.0g ox bloodProtein dissolution obtains in 100ml water.
More specifically, described developer, by 1.0-1.5g tetramethyl benzidine, adds after the dissolving of 50mL distilled water, then adds 20-40mgHorseradish peroxidase dissolves and obtains.
A preparation method for hydrogen peroxide quick detection test paper, concrete steps are as follows:
Step 1. antioxidant system preparation: take 0.5-1.5g Tea Polyphenols and 0.1-0.3g vitamin c, be dissolved in 100mL distillationIn water, then add 1.0-2.0g sodium thiosulfate, for subsequent use after stirring and dissolving;
Step 2. enzyme protection system preparation: take 2.0-3.5g sucrose and dissolve in 100mL distilled water, then add 0.5-1.5gPolyethylene glycol and 0.5-1.0g bovine serum albumin(BSA) heating for dissolving on electric furnace, cooling, for subsequent use;
Step 3. developer preparation: take 1.0-1.5g tetramethyl benzidine, add after the dissolving of 50mL distilled water, then add 20-40mgHorseradish peroxidase dissolves, for subsequent use;
Step 4. is mixed the antioxidant system preparing, enzyme protection system and developer, stirs, and obtains test paper soak;
Step 5. adopts test paper soak to soak filter paper;
After soaking in test paper soak, takes out by step 6. filter paper, and dry;
Filter paper is cut into filter paper bar by step 7., and filter paper bar is sticked on the substrate that posts double faced adhesive tape, cuts out detection by specificationTest paper finished product.
More specifically, test paper soaks environment: be 5-30 DEG C in temperature, humidity is under 40%-70% condition, and drying in the shade, chamber is avoidedHigh light soaks.
More specifically, after filter paper soaks, 35-45 DEG C of vacuum drying.
More specifically, must not there be burr the cut place of filter paper bar.
Test paper of the present invention, detection principle is: at hydrogen peroxide (H2O2) exist condition under, tetramethyl benzidine (TMB)After horseradish peroxidase enzyme catalytic, generate glaucous compound.
Advantage of the present invention: prepared antioxidant system with Tea Polyphenols, vitamin c, sodium thiosulfate, by sucrose, poly-second twoAlcohol, bovine serum albumin(BSA) are dissolved in water and have prepared enzyme protection system, by enzyme protection system protection horseradish peroxidase, pass throughAntioxidant system has been protected in tetramethyl benzidine easily oxidized group, makes the hydrogen peroxide quick detection test paper can be at normal temperatureLower preservation 1 year.
Brief description of the drawings
Fig. 1 is schematic diagram of the present invention.
Detailed description of the invention
Required equipment
Glass plate (20*35cm), beaker, glass bar, tetramethyl benzidine (TMB) (ARsigma), horseradish peroxidase(ARsigma), hydrochloric acid (AR Tianjin wind ship), vitamin c (ARsigma), Tea Polyphenols (traditional Chinese medicines reagent), ox blood is pureAlbumen (BRsigma), sucrose (ARsigma), pH test paper body paper.
Embodiment 1
Step 1. antioxidant system preparation: take 1.0000g Tea Polyphenols and 0.2000g vitamin c, be dissolved in 100mL distilled waterIn, then add 1.5000g sodium thiosulfate, for subsequent use after stirring and dissolving;
Step 2. enzyme protection system preparation: take 2.5000g sucrose and dissolve in 100mL distilled water, then add 1.0000gPolyethylene glycol and 0.8000g bovine serum albumin(BSA) heating for dissolving on electric furnace, cooling, for subsequent use;
Step 3. developer preparation: take 1.0000g tetramethyl benzidine, add after the dissolving of 50mL distilled water, then add 20mgHorseradish peroxidase dissolves, for subsequent use;
Step 4. is mixed the antioxidant system preparing, enzyme protection system and developer, stirs, and obtains test paper soak;
Step 5. is poured test paper soak in glass plate into, and the filter paper cutting out (pH test paper body paper, specification is 15*30cm) is slowSlow immersion in test paper soak, makes not occur bubble in the middle of filter paper and test paper soak as far as possible, and makes test paper soak not there be filter paper(every 100mL can soak 1 filter paper). Test paper soaks environment: be 5-30 DEG C in temperature, humidity is under 40%-70% condition,Avoid high light soaks in the chamber of drying in the shade.
Step 6. filter paper takes out soak 30min in test paper soak after, is laid on clean glass plate or PVC plate, 40Vacuum drying 3 hours at DEG C.
Step 7. cuts into the rectangular of the wide 8.9-9.1mm of being by above-mentioned specification by dried filter paper on instrument, at filter paper barMust not there be burr cut place, filter paper bar is sticked on the PVC plate that posts double faced adhesive tape, and be placed in automatic gas cutting machine, by specificationCut out Test paper finished product, length is 69-71mm, and wide is 5.9-6.1mm thickness 0.35mm. As shown in Figure 1, gained inspectionTest paper comprises substrate 2 (cutting of PVC plate forms), test-paper 1 (being formed by the cutting of filter paper bar), and one end of substrate 2 is hand-heldEnd 4, the other end is test-paper paste-side 3, pastes test-paper 1 on test-paper paste-side 3.
Adopt the hydrogen peroxide Test paper of preparation in this way, by enzyme protection system protection horseradish peroxidase, pass through antioxygenChange system has been protected easy oxidized group in tetramethyl benzidine, and hydrogen peroxide quick detection test paper can be protected at normal temperaturesDeposit 1 year.
Embodiment 2
Step 1. antioxidant system preparation: take 1.0000g Tea Polyphenols and 0.2000g vitamin c, be dissolved in 100mL distilled waterIn, then add 1.0000g sodium thiosulfate, for subsequent use after stirring and dissolving;
Step 2. enzyme protection system preparation: take 2.5000g sucrose and dissolve in 100mL distilled water, then add 1.0000gPolyethylene glycol and 0.5000g bovine serum albumin(BSA) heating for dissolving on electric furnace, cooling, for subsequent use;
Step 3. developer preparation: take 1.0000g tetramethyl benzidine, add after the dissolving of 50mL distilled water, then add 20mgHorseradish peroxidase dissolves, for subsequent use;
Step 4. is mixed the antioxidant system preparing, enzyme protection system and developer, stirs, and obtains test paper soak;
Step 5. is poured test paper soak in glass plate into, and the filter paper cutting out (pH test paper body paper, specification is 15*30cm) is slowSlow immersion in test paper soak, makes not occur bubble in the middle of filter paper and test paper soak as far as possible, and makes test paper soak not there be filter paper(every 100mL can soak 1 filter paper). Test paper soaks environment: be 5-30 DEG C in temperature, humidity is under 40%-70% condition,Avoid high light soaks in the chamber of drying in the shade.
Step 6. filter paper takes out soak 30min in test paper soak after, is laid on clean glass plate or PVC plate, 35Vacuum drying 3 hours at DEG C.
Step 7. cuts into the rectangular of the wide 8.9-9.1mm of being by above-mentioned specification by dried filter paper on instrument, at filter paper barMust not there be burr cut place, filter paper bar is sticked on the PVC plate that posts double faced adhesive tape, and be placed in automatic gas cutting machine, by specificationCut out Test paper finished product.
Embodiment 3
Step 1. antioxidant system preparation: take 1.0000g Tea Polyphenols and 0.1000g vitamin c, be dissolved in 100mL distilled waterIn, then add 2.0000g sodium thiosulfate, for subsequent use after stirring and dissolving;
Step 2. enzyme protection system preparation: take 2.5000g sucrose and dissolve in 100mL distilled water, then add 0.5000gPolyethylene glycol and 1.0000g bovine serum albumin(BSA) heating for dissolving on electric furnace, cooling, for subsequent use;
Step 3. developer preparation: take 1.0000g tetramethyl benzidine, add after the dissolving of 50mL distilled water, then add 40mgHorseradish peroxidase dissolves, for subsequent use;
Step 4. is mixed the antioxidant system preparing, enzyme protection system and developer, stirs, and obtains test paper soak;
Step 5. is poured test paper soak in glass plate into, and the filter paper cutting out (pH test paper body paper, specification is 15*30cm) is slowSlow immersion in test paper soak, makes not occur bubble in the middle of filter paper and test paper soak as far as possible, and makes test paper soak not there be filter paper(every 100mL can soak 1 filter paper). Test paper soaks environment: be 5-30 DEG C in temperature, humidity is under 40%-70% condition,Avoid high light soaks in the chamber of drying in the shade.
Step 6. filter paper takes out soak 30min in test paper soak after, is laid on clean glass plate or PVC plate, 45Vacuum drying 3 hours at DEG C.
Step 7. cuts into dried filter paper wide for rectangular on instrument by above-mentioned specification, must not have in the cut place of filter paper barBurr, stick on filter paper bar on the PVC plate that posts double faced adhesive tape, and are placed in automatic gas cutting machine, cut out and detect examination by specificationPaper product.
Embodiment 4
Step 1. antioxidant system preparation: take 1.5000g Tea Polyphenols and 0.3000g vitamin c, be dissolved in 100mL distilled waterIn, then add 1.5000g sodium thiosulfate, for subsequent use after stirring and dissolving;
Step 2. enzyme protection system preparation: take 2.0000g sucrose and dissolve in 100mL distilled water, then add 1.5000gPolyethylene glycol and 0.8000g bovine serum albumin(BSA) heating for dissolving on electric furnace, cooling, for subsequent use;
Step 3. developer preparation: take 1.5000g tetramethyl benzidine, add after the dissolving of 50mL distilled water, then add 20mgHorseradish peroxidase dissolves, for subsequent use;
Step 4. is mixed the antioxidant system preparing, enzyme protection system and developer, stirs, and obtains test paper soak;
Step 5. is poured test paper soak in glass plate into, and the filter paper cutting out (pH test paper body paper, specification is 15*30cm) is slowSlow immersion in test paper soak, makes not occur bubble in the middle of filter paper and test paper soak as far as possible, and makes test paper soak not there be filter paper(every 100mL can soak 1 filter paper). Test paper soaks environment: be 5-30 DEG C in temperature, humidity is under 40%-70% condition,Avoid high light soaks in the chamber of drying in the shade.
Step 6. filter paper takes out soak 30min in test paper soak after, is laid on clean glass plate or PVC plate, 40Vacuum drying 2 hours at DEG C.
Step 7. cuts into dried filter paper wide rectangular on instrument by above-mentioned specification, must not hairiness in the cut place of filter paper barLimit, sticks on filter paper bar on the PVC plate that posts double faced adhesive tape, and is placed in automatic gas cutting machine, cuts out Test paper by specificationFinished product.
Embodiment 5
Step 1. antioxidant system preparation: take 0.5000g Tea Polyphenols and 0.2000g vitamin c, be dissolved in 100mL distilled waterIn, then add 1.5000g sodium thiosulfate, for subsequent use after stirring and dissolving;
Step 2. enzyme protection system preparation: take 3.5000g sucrose and dissolve in 100mL distilled water, then add 1.0000gPolyethylene glycol and 0.8000g bovine serum albumin(BSA) heating for dissolving on electric furnace, cooling, for subsequent use;
Step 3. developer preparation: take 0.8000g tetramethyl benzidine, add after the dissolving of 50mL distilled water, then add 20mgHorseradish peroxidase dissolves, for subsequent use;
Step 4. is mixed the antioxidant system preparing, enzyme protection system and developer, stirs, and obtains test paper soak;
Step 5. is poured test paper soak in glass plate into, and the filter paper cutting out (pH test paper body paper, specification is 15*30cm) is slowSlow immersion in test paper soak, makes not occur bubble in the middle of filter paper and test paper soak as far as possible, and makes test paper soak not there be filter paper(every 100mL can soak 1 filter paper). Test paper soaks environment: be 5-30 DEG C in temperature, humidity is under 40%-70% condition,Avoid high light soaks in the chamber of drying in the shade.
Step 6. filter paper takes out soak 30min in test paper soak after, is laid on clean glass plate or PVC plate, 40Vacuum drying 3 hours at DEG C.
Step 7. cuts into the rectangular of the wide 8.9-9.1mm of being by above-mentioned specification by dried filter paper on instrument, at filter paper barMust not there be burr cut place, filter paper bar is sticked on the PVC plate that posts double faced adhesive tape, and be placed in automatic gas cutting machine, by specificationCut out Test paper finished product.
Claims (9)
1. a hydrogen peroxide quick detection test paper, comprises substrate, test-paper, and one end of substrate is handheld terminal, and the other end is that test-paper is stickyPaste end, test-paper is pasted test-paper on paste-side, and test-paper, after test paper soak soaks, is dried and obtains; It is characterized in that:Described test paper soak is mixed by antioxidant system, enzyme protection system and developer, described antioxidant system by Tea Polyphenols,Vitamin c, sodium thiosulfate are dissolved in water and obtain.
2. hydrogen peroxide quick detection test paper according to claim 1, is characterized in that: described enzyme protection system is by sucrose, poly-secondGlycol, bovine serum albumin(BSA) are dissolved in water and obtain.
3. hydrogen peroxide quick detection test paper according to claim 1, is characterized in that: described antioxidant system is by 0.5-1.5gTea Polyphenols, 0.1-0.3g vitamin c, 1.0-2.0g sodium thiosulfate are dissolved in 100ml water and obtain.
4. hydrogen peroxide quick detection test paper according to claim 2, is characterized in that: described enzyme protection system is by 2.0-3.5gSucrose, 0.5-1.5g polyethylene glycol, 0.5-1.0g bovine serum albumin(BSA) are dissolved in 100ml water and obtain.
5. hydrogen peroxide quick detection test paper according to claim 1, is characterized in that: described developer is by 1.0-1.5g tetramethylBase benzidine, adds after the dissolving of 50mL distilled water, then adds the dissolving of 20-40mg horseradish peroxidase to obtain.
6. a preparation method for hydrogen peroxide quick detection test paper described in claim 1, is characterized in that: concrete steps are as follows:
Step 1. antioxidant system preparation: take 0.5-1.5g Tea Polyphenols and 0.1-0.3g vitamin c, in 100mL distilled waterIn, then add 1.0-2.0g sodium thiosulfate, for subsequent use after stirring and dissolving;
Step 2. enzyme protection system preparation: take 2.0-3.5g sucrose and dissolve in 100mL distilled water, then add 0.5-1.5gPolyethylene glycol and 0.5-1.0g bovine serum albumin(BSA) heating for dissolving on electric furnace, cooling, for subsequent use;
Step 3. developer preparation: take 1.0-1.5g tetramethyl benzidine, add after the dissolving of 50mL distilled water, then add 20-40mgHorseradish peroxidase dissolves, for subsequent use;
Step 4. is mixed the antioxidant system preparing, enzyme protection system and developer, stirs, and obtains test paper soak;
Step 5. adopts test paper soak to soak filter paper;
After soaking in test paper soak, takes out by step 6. filter paper, and dry;
Filter paper is cut into filter paper bar by step 7., and filter paper bar is sticked on the substrate that posts double faced adhesive tape, cuts out detection by specificationTest paper finished product.
7. the preparation method of hydrogen peroxide quick detection test paper according to claim 6, is characterized in that: test paper soaks environment: in temperatureDegree is 5-30 DEG C, and humidity is under 40%-70% condition, avoids high light soak in the chamber of drying in the shade.
8. the preparation method of hydrogen peroxide quick detection test paper according to claim 6, is characterized in that: after filter paper soaks, at 35-45DEG C vacuum drying.
9. the preparation method of hydrogen peroxide quick detection test paper according to claim 6, is characterized in that: the cut place of filter paper bar must notThere are burr.
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| CN105891202A (en) * | 2016-06-03 | 2016-08-24 | 广州市进德生物科技有限公司 | Single-component TMB color developing solution and preparation method thereof |
| CN107121429A (en) * | 2017-06-15 | 2017-09-01 | 公安部物证鉴定中心 | A kind of sodium azide quick detection test paper and its application |
| CN107356588A (en) * | 2017-06-30 | 2017-11-17 | 济南大学 | The superoxide radical and hydrogen peroxide paper substrate detection method of a kind of simple and sensitive |
| CN107917910A (en) * | 2017-11-16 | 2018-04-17 | 武汉轻工大学 | It is a kind of to be used to detect class enzyme test peper of hydrogen peroxide and its preparation method and application |
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| CN109507176A (en) * | 2018-11-12 | 2019-03-22 | 长春理工大学 | Dry chemical hydrogen peroxide detection method based on secondary heme hexapeptide |
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| CN107356588A (en) * | 2017-06-30 | 2017-11-17 | 济南大学 | The superoxide radical and hydrogen peroxide paper substrate detection method of a kind of simple and sensitive |
| CN107917910A (en) * | 2017-11-16 | 2018-04-17 | 武汉轻工大学 | It is a kind of to be used to detect class enzyme test peper of hydrogen peroxide and its preparation method and application |
| CN109254001B (en) * | 2018-10-29 | 2021-06-04 | 青岛农业大学 | Preparation and application of starch rapid test card |
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