CN112715749A - Trachinotus ovatus feed additive capable of improving immunity - Google Patents
Trachinotus ovatus feed additive capable of improving immunity Download PDFInfo
- Publication number
- CN112715749A CN112715749A CN202011256358.2A CN202011256358A CN112715749A CN 112715749 A CN112715749 A CN 112715749A CN 202011256358 A CN202011256358 A CN 202011256358A CN 112715749 A CN112715749 A CN 112715749A
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- trachinotus ovatus
- feed
- bacillus subtilis
- fructo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Birds (AREA)
- Marine Sciences & Fisheries (AREA)
- Insects & Arthropods (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a trachinotus ovatus feed additive for improving immunity. The additive mainly comprises the following substances in parts by weight: 7-8 parts of bacillus subtilis and 4-8 parts of fructo-oligosaccharide. Also disclosed is a method of use of the invention: every 2000 parts of feed is added with 7-8 parts of bacillus subtilis and 4-8 parts of fructo-oligosaccharide. When in use, the feed is mixed with the trachinotus ovatus feed uniformly, and then the trachinotus ovatus is fed with the feed every day, so that the immunity and the disease resistance of the trachinotus ovatus can be improved. The bacillus subtilis is a probiotic for aquaculture, and can improve the digestion capacity and immunity of organisms and inhibit the growth of pathogenic bacteria in intestinal tracts. The fructo-oligosaccharide has certain immunogenicity, can stimulate the immune response of organisms, and simultaneously has the function of promoting the proliferation of beneficial bacteria. The interaction of the two components can improve the growth performance, immunity and disease resistance of trachinotus ovatus.
Description
Technical Field
The invention relates to a trachinotus ovatus feed additive for improving immunity, and belongs to the technical field of marine aquaculture.
Background
Trachinotus ovatus (arch of Trachinotus ovatus)Trachinotus ovatus) Commonly known as golden pomfretThe edible fish is distributed in temperate and tropical sea areas of Indian ocean, Pacific ocean and Atlantic ocean, is a high-quality edible fish, gradually becomes a main variety for seawater cage culture in south China in recent years, and the culture has already formed scale.
At present, golden pomfret breeding density is high, due to rapid development and disordered expansion of seawater breeding, sea areas are overloaded, breeding environments are continuously worsened, and due to the lack of scientific breeding management in the breeding process, diseases frequently occur, and various pathogenic pathogens also come with the seawater. The traditional method for controlling diseases by adopting antibiotics and chemical drugs is easy to cause environmental pollution, drug residues and drug-resistant germs. Therefore, with the increasing demand for environment-friendly aquaculture, it is important to find alternatives to antibiotics.
Disclosure of Invention
The invention aims to overcome the defect that the disease is controlled by adopting antibiotics and chemical drugs in the process of culturing the trachinotus ovatus. Provides a feed additive for improving the immunity and disease resistance of trachinotus ovatus.
In order to solve the problems, the following technical scheme is adopted: a trachinotus ovatus feed additive for improving immunity mainly comprises the following substances in parts by weight: 7-8 parts of bacillus subtilis and 4-8 parts of fructo-oligosaccharide.
The bacillus subtilis freeze-dried powder has the bacterium content not lower than 1.5 multiplied by 1010cfu/g。
The application method of the trachinotus ovatus feed additive for improving immunity comprises the following steps: every 2000 parts of feed is added with 7-8 parts of bacillus subtilis and 4-8 parts of fructo-oligosaccharide.
The feed is a commercial aquaculture feed.
The bacillus subtilis is a probiotic for aquaculture, and can improve the digestion capacity and immunity of organisms and inhibit the growth of pathogenic bacteria in intestinal tracts. The fructo-oligosaccharide has certain immunogenicity, can stimulate the immune response of organisms, and simultaneously has the function of promoting the proliferation of beneficial bacteria. Therefore, by utilizing the interaction of the trachinotus ovatus and the carangid, the immunity and the disease resistance of the trachinotus ovatus are improved.
Detailed Description
Example one
70g of bacillus subtilis and 40g of fructo-oligosaccharide are selected and added into 20kg of the Hexing feed and mixed evenly. 360 trachinotus ovatus larvae are respectively cultured in 6 2.0 multiplied by 1.5m net cages, the trachinotus ovatus with 3 net cages are fed twice at 5 and 18 points every day, and the other 3 net cages are only fed with common commercial feed. After 8 weeks of breeding, the specific growth rate, feed conversion rate, respiratory burst activity, phagocytic activity and lysozyme activity of trachinotus ovatus under two feeding modes are respectively determined, and infection experiments are carried out at the end of 8 weeks of breeding by using a semi-lethal concentration of 1.9 × 106And (5) injecting the trachinotus ovatus into the abdominal cavity of the vibrio vulnificus of cfu/ml, and calculating the 10-day cumulative mortality. The results show that the specific growth rate, feed conversion rate, respiratory burst activity, phagocytic activity and lysozyme activity of the fish added with the bacillus subtilis and the fructo-oligosaccharide are all obviously improved compared with the fish not added with the bacillus subtilis and the fructo-oligosaccharide, the cumulative death rate of the fish infected with the vibrio vulnificus within 10 days is only 29.63 percent, and the death rate of the fish fed with the common commercial feed only reaches 75.93 percent.
Example two
80g of bacillus subtilis and 80g of fructo-oligosaccharide are selected and added into 20kg of the Hengxing feed and mixed evenly. 360 young trachinotus ovatus are respectively cultured in 6 net cages of 2.0 multiplied by 1.5, 3 net cages of the trachinotus ovatus are fed with food twice at 5 and 18 points every day, and the other 3 net cages are only fed with common commercial feed. After 8 weeks of feeding, specific growth rate, feed conversion rate, respiratory burst activity, phagocytic activity and lysozyme activity were measured. Infection experiments were performed at the end of 8 weeks of culture with a semi-lethal concentration of 1.9X 106And (5) injecting the trachinotus ovatus into the abdominal cavity of the vibrio vulnificus of cfu/ml, and calculating the 10-day cumulative mortality. The results show that the specific growth rate, feed conversion rate, respiratory burst activity, phagocytic activity and lysozyme activity of the experimental fish added with the bacillus subtilis and the fructo-oligosaccharide are all obviously improved compared with the fish not added with the bacillus subtilis and the fructo-oligosaccharide, the cumulative death rate of the experimental fish infected with the vibrio vulnificus within 10 days is only 33.33%, and the death rate of the experimental fish fed with the common commercial feed only reaches 75.93%.
Claims (3)
1. A trachinotus ovatus feed additive for improving immunity is characterized in that: the additive mainly comprises the following substances in parts by weight: 7-8 parts of bacillus subtilis and 4-8 parts of fructo-oligosaccharide.
2. The trachinotus ovatus feed additive for improving immunity as claimed in claim 1, wherein the feed additive comprises: the bacillus subtilis freeze-dried powder has the bacterium content not lower than 1.5 multiplied by 1010cfu/g。
3. A use method of a trachinotus ovatus feed additive for improving immunity is characterized in that: every 2000 parts of feed is added with 7-8 parts of bacillus subtilis and 4-8 parts of fructo-oligosaccharide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011256358.2A CN112715749A (en) | 2020-11-11 | 2020-11-11 | Trachinotus ovatus feed additive capable of improving immunity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011256358.2A CN112715749A (en) | 2020-11-11 | 2020-11-11 | Trachinotus ovatus feed additive capable of improving immunity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112715749A true CN112715749A (en) | 2021-04-30 |
Family
ID=75597457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011256358.2A Pending CN112715749A (en) | 2020-11-11 | 2020-11-11 | Trachinotus ovatus feed additive capable of improving immunity |
Country Status (1)
| Country | Link |
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| CN (1) | CN112715749A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115553402A (en) * | 2022-10-25 | 2023-01-03 | 山东深海生物科技股份有限公司 | Biological polypeptide preparation capable of improving nonspecific immunity function of haliotis discus hannai and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0739318A (en) * | 1993-07-29 | 1995-02-10 | Maruha Corp | Feed for fish |
| CN105368749A (en) * | 2015-12-04 | 2016-03-02 | 青岛玛斯特生物技术有限公司 | Bacillus subtilis and feed additive containing same |
| CN107858302A (en) * | 2017-11-21 | 2018-03-30 | 华南农业大学 | A kind of bacillus subtilis 7K and its application |
| CN111972326A (en) * | 2020-09-04 | 2020-11-24 | 广西海洋研究所有限责任公司 | Method for breeding stichopus japonicus by using mating flowers in intermediate cultivation of Sipunculus nudus seedlings |
-
2020
- 2020-11-11 CN CN202011256358.2A patent/CN112715749A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0739318A (en) * | 1993-07-29 | 1995-02-10 | Maruha Corp | Feed for fish |
| CN105368749A (en) * | 2015-12-04 | 2016-03-02 | 青岛玛斯特生物技术有限公司 | Bacillus subtilis and feed additive containing same |
| CN107858302A (en) * | 2017-11-21 | 2018-03-30 | 华南农业大学 | A kind of bacillus subtilis 7K and its application |
| CN111972326A (en) * | 2020-09-04 | 2020-11-24 | 广西海洋研究所有限责任公司 | Method for breeding stichopus japonicus by using mating flowers in intermediate cultivation of Sipunculus nudus seedlings |
Non-Patent Citations (4)
| Title |
|---|
| 冀贞阳: "含"生长因子"的饲料和饲料添加剂", 《畜禽业》 * |
| 刘宇等: "鱼虾肠道菌群代谢产物短链脂肪酸研究进展", 《生物技术通报》 * |
| 江波 等: "功能性饲料添加剂——低聚果糖", 《粮食与饲料工业》 * |
| 陈琳等: "实时定量PCR法快速检测水产品中的副溶血性弧菌", 《浙江农业学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115553402A (en) * | 2022-10-25 | 2023-01-03 | 山东深海生物科技股份有限公司 | Biological polypeptide preparation capable of improving nonspecific immunity function of haliotis discus hannai and application thereof |
| CN115553402B (en) * | 2022-10-25 | 2024-04-05 | 山东深海生物科技股份有限公司 | Biological polypeptide preparation capable of improving nonspecific immunity function of Haliotis discus hand-Mazz and application thereof |
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