CN112689674A - 葡聚糖亲和性标签及其应用 - Google Patents
葡聚糖亲和性标签及其应用 Download PDFInfo
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- CN112689674A CN112689674A CN201880097424.0A CN201880097424A CN112689674A CN 112689674 A CN112689674 A CN 112689674A CN 201880097424 A CN201880097424 A CN 201880097424A CN 112689674 A CN112689674 A CN 112689674A
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/13—Labelling of peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
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- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
提供了一种亲和性标签，其为葡聚糖结合功能区块的片段，可通过与葡聚糖的亲和力纯化目标蛋白质。所述亲和性标签可广泛应用于需要蛋白质纯化工艺的产业应用中。
Description
PCT国内申请，说明书已公开。
Claims (15)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2018/112303 WO2020087194A1 (zh) | 2018-10-29 | 2018-10-29 | 葡聚糖亲和性标签及其应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112689674A true CN112689674A (zh) | 2021-04-20 |
| CN112689674B CN112689674B (zh) | 2024-05-28 |
Family
ID=70461852
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880097424.0A Active CN112689674B (zh) | 2018-10-29 | 2018-10-29 | 葡聚糖亲和性标签及其应用 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20220002353A1 (zh) |
| CN (1) | CN112689674B (zh) |
| WO (1) | WO2020087194A1 (zh) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225378A (zh) * | 2008-01-23 | 2008-07-23 | 广西大学 | 内切葡聚糖酶和它的编码基因与应用 |
| CN101809031A (zh) * | 2007-09-27 | 2010-08-18 | 塞诺菲-安万特股份有限公司 | 核受体的可控形式的配体结合结构域以及涉及它的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1672075A1 (en) * | 2004-12-17 | 2006-06-21 | Bayer CropScience GmbH | Transformed plant expressing a dextransucrase and synthesizing a modified starch |
| RU2428477C2 (ru) * | 2009-05-15 | 2011-09-10 | Дмитрий Викторович Гришин | РЕКОМБИНАНТНАЯ БЕЛКОВАЯ КОНСТРУКЦИЯ ДСД-сп-β-ГАЛ, ОБЛАДАЮЩАЯ АКТИВНОСТЬЮ ФЕРМЕНТА ТЕРМОСТАБИЛЬНОЙ β-ГАЛАКТОЗИДАЗЫ (ЛАКТАЗЫ) И СПОСОБНОСТЬЮ АФФИННО СВЯЗЫВАТЬСЯ С ДЕКСТРАНОМ, ПЛАЗМИДНАЯ ДНК pGD-10, ОПРЕДЕЛЯЮЩАЯ БИОСИНТЕЗ ДСД-сп-β-ГАЛ, И ШТАММ-ПРОДУЦЕНТ Escherichia coli DH5α/PGD-10 |
| CN102757501A (zh) * | 2011-04-29 | 2012-10-31 | 中国科学院上海生命科学研究院 | 蛋白标签的联合应用 |
| US10745675B2 (en) * | 2017-05-02 | 2020-08-18 | The United States Of America, As Represented By The Secretary Of Agriculture | Modified enzymes for producing increased isomelezitose |
-
2018
- 2018-10-29 CN CN201880097424.0A patent/CN112689674B/zh active Active
- 2018-10-29 US US17/289,475 patent/US20220002353A1/en active Pending
- 2018-10-29 WO PCT/CN2018/112303 patent/WO2020087194A1/zh active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101809031A (zh) * | 2007-09-27 | 2010-08-18 | 塞诺菲-安万特股份有限公司 | 核受体的可控形式的配体结合结构域以及涉及它的方法 |
| CN101225378A (zh) * | 2008-01-23 | 2008-07-23 | 广西大学 | 内切葡聚糖酶和它的编码基因与应用 |
Non-Patent Citations (3)
| Title |
|---|
| "hydrolase [Leuconostoc mesenteroides], ACCESSION: WP_084014924.1", 《GENPEPT》, pages 1 - 2 * |
| KUNIYOSHI KASEDA等: "A novel approach for puri¢cation of recombinant proteins using the dextran-binding domain", 《FEBS LETTERS》, vol. 500, pages 141 * |
| 哈依努尔等: "翼螺旋转录因子FOXO1的 DNA 结合域的表达、纯化及结合特性", 《中南大学学报（医学版）》, vol. 42, no. 1, pages 1 - 7 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20220002353A1 (en) | 2022-01-06 |
| CN112689674B (zh) | 2024-05-28 |
| WO2020087194A1 (zh) | 2020-05-07 |
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