CN112675577A - Extraction device and method for extracting platelet-rich plasma from blood - Google Patents
Extraction device and method for extracting platelet-rich plasma from blood Download PDFInfo
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- CN112675577A CN112675577A CN201910987018.8A CN201910987018A CN112675577A CN 112675577 A CN112675577 A CN 112675577A CN 201910987018 A CN201910987018 A CN 201910987018A CN 112675577 A CN112675577 A CN 112675577A
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Abstract
The present invention relates to an extraction device and method for extracting platelet-rich plasma from blood. The device includes: a first container, a second container and a switch member. The first intercommunication mouth of sealing connection first container and the second intercommunication mouth of second container can be dismantled respectively at switch both ends and be equipped with the passageway in the switch. Wherein the switch member is switchable between at least two positions, a first switching position in which the first container and the second container are in communication via the passage in the switch member, contents in the first chamber being transferred to the second chamber under sliding movement of the first regulating member to extract platelet rich plasma from blood contained in the first container, and a second switching position in which communication between the first container and the second container is interrupted by the switch member.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to an extraction device and method for extracting platelet-rich plasma from blood.
Background
After centrifugation, the blood is divided into three layers, namely a Platelet-poor Plasma (PPP) layer, a Platelet-Rich Plasma (PRP) layer and a red blood cell layer from top to bottom. Among them, PRP has the effects of accelerating wound healing and repairing cartilage damage, reducing the formation of surgical scars and promoting cartilage regeneration, and thus is widely used in the medical field.
In the prior art, two times of centrifugation are generally adopted to extract PRP, blood plasma (including PRP and PPP) is obtained after the first centrifugation, and PRP is obtained after the second centrifugation, but the extraction process is generally operated in a test tube, various medical consumables and specially designed and manufactured extraction devices are required to be matched for use, the extracted PRP is easily polluted, the operation is complex, and the efficiency is low.
Disclosure of Invention
The present invention aims to provide an extraction device for extracting platelet-rich plasma from blood that can solve at least some of the above problems.
The present invention is also directed to a method of extracting platelet rich plasma from blood using the above improved extraction device for extracting platelet rich plasma from blood.
According to an aspect of the present invention, there is provided an extraction device for extracting platelet-rich plasma from blood, comprising: the first container comprises a first outer wall, a first adjusting piece sliding along the inner side of the first outer wall in a sealing mode, a first chamber defined by the first outer wall and the first adjusting piece, and a first communication port penetrating through the first outer wall and communicated with the first chamber; the second container comprises a second outer wall, a sealing end piece arranged on the second outer wall, a second chamber enclosed by the second outer wall and the sealing end piece, and a second communication port penetrating through the second outer wall and communicated with the second chamber; the two ends of the switch piece are respectively detachably and hermetically connected with the first communicating port of the first container and the second communicating port of the second container, and a channel is arranged in the switch piece; wherein the switch member is switchable between at least two positions, a first switching position in which the first and second containers are in communication via the passageway in the switch member and the contents of the first chamber are transferred to the second chamber under sliding movement of the first adjustment member to extract platelet rich plasma from the blood contained in the first container, and a second switching position in which communication between the first and second containers is interrupted by the switch member.
Compared with the prior art, the extraction of the PRP can be realized by combining the first container, the second container and the switch member and operating the first adjusting member of the first container in the embodiment, so that the risk of introducing pollution is reduced, the process of extracting the PRP is greatly simplified, and the required manufacturing cost is reduced. And the transfer of the contents in the first container and the second container is realized by the pushing of the first adjusting piece, so that the interlayer disturbance is greatly reduced, and the PRP can be more clearly extracted. Finally, the concentration of the extracted PRP can be controlled by controlling the propelling depth of the second regulating member, and the clinical application range is expanded.
Preferably, the seal end member is configured as a second regulating member sealingly sliding along the inside of the second outer wall, and in the first switching position, the contents in the first chamber and the contents in the second chamber are displaced from each other by the sliding of the first regulating member and the second regulating member.
Preferably, the switch member is configured as a two-way valve.
Preferably, the first container and/or the second container is configured as a syringe.
Preferably, the switch member further has a vent end, the switch member being switchable between at least three positions, a first switching position in which the first and second containers are in communication via the passage in the switch member and communication between the vent end and the second container is interrupted by the switch member, a second switching position in which communication between the first and second containers and communication between the vent end and the second container are both interrupted by the switch member, and a third switching position in which communication between the first and second containers is interrupted by the switch member and the vent end and the second container are in communication via the passage in the switch member.
Preferably, the switch is configured as a three-way valve.
Preferably, a first seal is removably attached to a free end of the vent end.
Preferably, one end of the switch piece, which is correspondingly connected with the first communication port, is provided with a first locking piece, and the first communication port is provided with a first matching locking piece which is matched with the first locking piece to lock the switch piece and the first container; the switch piece corresponds the one end of connecting the second intercommunication mouth and is equipped with the second locking piece, the second intercommunication mouth be equipped with the cooperation of second locking piece is in order to lock the switch piece with the second of second container pairs the locking piece.
Preferably, the first locking member is configured as a male luer/female luer connector and the first mating locking member is configured as a female luer/male luer connector; the second locking member is configured as a luer male/female connector and the second mating locking member is configured as a luer female/luer male connector.
Preferably, the first container is configured such that when the switch member is removed, a second sealing member is removably attached to a free end of the first mating locking member; the second container is configured such that when the switch member is removed, a third sealing member is removably attached to a free end of the second mating locking member.
Preferably, the connection between the first locking means and the first counter locking means and the connection between the second locking means and the second counter locking means can be designed as a screw thread engagement and/or a snap fit engagement.
Preferably, the first and/or second container, the first and/or first counterpart lock and the second and/or second counterpart lock are constructed from a transparent material.
According to another aspect of the present invention, there is provided a method for extracting platelet rich plasma from blood using the aforementioned extraction device for extracting platelet rich plasma from blood, the method comprising: placing the first communication port of the first container containing the collected blood upward for first centrifugation, wherein the contents in the first chamber are divided into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom; sealingly connecting the first and second containers to the switch and causing the switch to be switched in a first switching position, pushing the first regulating member to transfer the platelet poor plasma layer in the first chamber into the second chamber free of contents of the second container via the channel in the switch; switching the switch member to a second switching position and detaching a second container containing a platelet poor plasma layer from the switch member; and replacing the second container containing the platelet-poor plasma layer with a new second container containing no content, connecting the new second container containing no content to the switch member in a sealing manner, switching the switch member to a first switching position, pushing the first adjusting member to push the platelet-rich plasma layer in the first chamber into the new second container containing no content, and extracting the platelet-rich plasma from the blood contained in the first container by only containing the platelet-rich plasma layer in the new second container containing no content.
According to still another aspect of the present invention, there is provided a method of extracting platelet rich plasma from blood using the aforementioned extraction device for extracting platelet rich plasma from blood, the method including: placing the first communication port of the first container containing the collected blood upward for first centrifugation, wherein the contents in the first chamber are divided into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom; sealingly connecting the first and second containers to the switch and causing the switch to be switched in a first switching position, pushing the first regulating member to transfer the platelet-poor and platelet-rich plasma layers in the first chamber into the second chamber free of contents of the second container via the channel in the switch; switching the switch piece to a second switching position and detaching the second container from the switch piece, so that the second communication port of the second container is placed upwards for second centrifugation, and the content in the second chamber is divided into a platelet poor plasma layer and a platelet rich plasma layer from top to bottom; and the second container is hermetically connected to the switch member, the switch member is switched to the first switching position, and the second adjusting member is pushed to transfer the platelet poor plasma layer in the second cavity into the first container, so that the second container only contains the platelet rich plasma layer to extract the platelet rich plasma from the blood contained in the first container.
Preferably, before switching the switching member to the second switching position and detaching the second container from the switching member, the method includes: switching the switch member to a third switching position, and pulling the second regulating member to draw air through the venting end to draw platelet rich plasma remaining in the switch member into the second chamber.
According to still another aspect of the present invention, there is provided a method of extracting platelet rich plasma from blood using the aforementioned extraction device for extracting platelet rich plasma from blood, the method including: placing the first container containing the collected blood with the first communication port facing downward for a first centrifugation, wherein the contents in the first chamber are divided into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom; sealingly connecting the first and second containers to the switch and causing the switch to be switched in a first switching position, urging the first regulating member to transfer the layer of red blood cells in the first chamber into the second chamber free of contents of the second container via the channel in the switch; switching the switch piece to a second switching position and detaching the first container from the switch piece, so that the first communication port of the first container is placed upwards for second centrifugation, and the content in the first chamber is divided into a platelet poor plasma layer and a platelet rich plasma layer from top to bottom; and the first container is hermetically connected to the switch member, the switch member is switched to a first switching position, and the first adjusting member is pushed to transfer the platelet poor plasma layer in the first cavity into the second container, so that the first container only contains the platelet rich plasma layer to extract the platelet rich plasma from the blood contained in the first container.
Additional features and advantages of the invention will be set forth in part in the description which follows, and in part will be apparent to those having ordinary skill in the art upon examination of the following, or may be learned from the practice of the invention.
Drawings
Embodiments of the invention are described in detail below with reference to the attached drawing figures, wherein:
fig. 1 is a schematic view of an extraction device for extracting platelet rich plasma from blood according to an embodiment of the present invention, with a switch member switched to a first switch position, wherein the contents of a first container are transferred to a second container;
FIG. 2 is a schematic view of an extraction device for extracting platelet rich plasma from blood according to an embodiment of the present invention with the switch member switched in a third switching position;
fig. 3 is a schematic view of an extraction device for extracting platelet rich plasma from blood according to an embodiment of the present invention with the switch member switched to a first switch position in which the contents of the second container are transferred to the first container;
fig. 4 is a schematic view of an extraction device for extracting platelet rich plasma from blood according to an embodiment of the present invention with the switching member switched to the second switching position.
Description of reference numerals:
1-an extraction device for extracting platelet rich plasma from blood; 11-a first container; 111-a first outer wall; 112-a first adjustment member; 113-a first chamber; 114-a first communication port; 12-a second container; 121-a second outer wall; 122-a seal end piece; 123-a second chamber; 124-a second communication port; 13-a switch member; 131-a venting end; 132-first seal.
Detailed Description
Referring now to the drawings, illustrative aspects of the disclosed apparatus and method will be described in detail. Although the drawings are provided to present some embodiments of the invention, the drawings are not necessarily to scale of particular embodiments, and certain features may be exaggerated, removed, or partially sectioned to better illustrate and explain the present disclosure. The position of some components in the drawings can be adjusted according to actual requirements on the premise of not influencing the technical effect. The appearances of the phrase "in the drawings" or similar language in the specification are not necessarily referring to all drawings or examples.
Certain directional terms used hereinafter to describe the drawings, such as "inner", "outer", "above", "below", and other directional terms, will be understood to have their normal meaning and refer to those directions as they normally relate to when viewing the drawings. Unless otherwise indicated, the directional terms described herein are generally in accordance with conventional directions as understood by those skilled in the art.
The terms "first," "second," and the like, as used herein, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another.
As used herein, the terms "joined," "connected," and the like, are intended to encompass both components which are indirectly joined together through intervening layers (e.g., adhesives, welds, etc.) or intermediate members (e.g., connectors, transitions, etc.), and components which are directly joined together without any intervening layers (e.g., adhesives, welds, etc.) or intermediate members (e.g., connectors, transitions, etc.).
Fig. 1 to 4 show, by way of example, an extraction device of the present invention for extracting platelet-rich plasma from blood. The extraction device 1 for extracting platelet-rich plasma from blood in this example involves fewer structural components and is simple to operate and efficient in extraction.
The extraction device 1 for extracting platelet-rich plasma from blood includes: the first container 11 includes a first outer wall 111, a first regulating member 112 that slides in a sealed manner along an inner side of the first outer wall 111, a first chamber 113 surrounded by the first outer wall 111 and the first regulating member 112, and a first communicating port 114 that penetrates the first outer wall 111 and communicates with the first chamber 113. And a second container 12 including a second outer wall 121, a seal end piece 122 provided on the second outer wall 121, a second chamber 123 enclosed by the second outer wall 121 and the seal end piece 122, and a second communication port 124 penetrating the second outer wall 121 and communicating with the second chamber 123. The opening/closing member 13 has both ends detachably and hermetically connected to the first communication port 114 of the first container 11 and the second communication port 124 of the second container 12, respectively, and a passage is provided in the opening/closing member 13. Wherein the switch member 13 is switchable between at least two positions, a first switching position in which the first container 11 and the second container 12 are in communication via the passage in the switch member 13, the contents of the first chamber 113 being transferred to the second chamber 123 under sliding movement of the first regulating member 112 to extract platelet rich plasma from the blood contained in the first container 11, and a second switching position in which communication between the first container 11 and the second container 12 is interrupted by the switch member 13.
Specifically, the volume of the first chamber 113 changes as the first regulation member 112 slides. When the first container 11 and the second container 12 are connectable to the switch 13 and the switch 13 is switched to the first switching position, the first chamber 113, the first communication port 114, the passage in the switch 13, the second communication port 124, and the second chamber 123 constitute a closed space that communicates. The first regulating member 112 is driven by an external force to slide toward the first communicating port 114 to reduce the volume of the first chamber 113, and the contents in the first chamber 113, such as PPP and PRP in blood, are sequentially transferred into the second chamber 123 of the second container 12. When the transfer of the contents of the first chamber 113 is not required, the switch 13 is switched to the second position to disconnect the communication between the first chamber 113 and the second chamber 123. In particular, first container 11 may be configured as a syringe and second container 12 may be configured as a test tube with seal piece 122 integrally formed with second outer wall 121.
Alternatively, the sealing end piece 122 is configured as a second regulating member that sealingly slides along the inside of the second outer wall 121, and in the first switching position, the contents in the first chamber 113 and the contents in the second chamber 123 are transferred to each other by the sliding of the first regulating member 112 and the second regulating member.
Specifically, the volume of the second chamber 123 is changed as the second regulating member slides. When the switch member 13 is switched to the first switching position, the first regulating member 112 is driven by an external force to slide toward the first communication port 114 to reduce the volume of the first chamber 113, the contents in the first chamber 113 such as PPP and PRP in blood are transferred into the second chamber 123 of the second container 12, and the second regulating member is forced to slide away from the second communication port 124 by the contents entering the second chamber 123, and the volume of the second chamber 123 is increased. Alternatively, when the switch member 13 is switched to the first switching position, the second regulating member is driven by an external force to slide toward the second communication port 124 to reduce the volume of the second chamber 123, the content such as PPP in the second chamber 123 is transferred into the first chamber 113 of the first container 11, and the first regulating member 112 is forced to slide away from the first communication port 114 by the content entering the first chamber 113, and the volume of the first chamber 113 is increased.
In particular, the first container 11 and/or the second container 12 can be configured as a syringe, and accordingly the first outer wall 111 and/or the second outer wall 121 is configured as a cylinder wall of the syringe, the first adjusting element 112 and/or the second adjusting rod is configured as a piston of the syringe, and the first communication opening 114 and/or the second communication opening 124 is configured as a conical head of the syringe. The injector has wide application in the field of medical appliances, simple and convenient structure and operation and low price, thereby greatly reducing the operation difficulty of the extracting device 1 for extracting platelet-rich plasma from blood and reducing the manufacturing cost. Wherein the first container 11 and/or the second container 12 can be a syringe made of transparent material such as plastic or glass, so as to facilitate the observation of the pushing and pulling conditions of the first regulating member 112 and the second regulating member and the contents of the first chamber 113 and the second chamber 123, thereby further facilitating the control of the separation process of the PRP. In addition, the first container 11 and/or the second container 12 may be configured as a pusher or a profile syringe or other container configurations conventional in the art.
Alternatively, the switching member 13 may be configured as a two-way valve for enabling and disabling communication between the first chamber 113 of the first container 11 and the second chamber 123 of the second container 12. Wherein the two-way valve may further be configured as a manually operated two-way valve comprising a two-way valve spool provided with a passage and a rotary piston controlling the rotation of the two-way valve spool, the first communication port 114 and the second communication port 124 being aligned by operating the rotary piston to be positioned in a first switching position such that the passage in the two-way valve spool is aligned with both ends of the two-way valve spool, thereby allowing the first container 11 and the second container 12 to communicate via the passage in the two-way valve spool, or the two-way valve spool blocks both ends of the two-way valve spool by positioning the rotary piston in a second switching position, thereby allowing the first container 11 and the second container 12 to be disconnected. In the practical application process, the two-way valve can also be in other conventional two-way valve structures, and the two-way valve has wide application in the field of medical instruments, simple and convenient structure operation and low price, so that the operation difficulty of the extraction device 1 for extracting platelet-rich plasma from blood in the embodiment is greatly reduced, and the manufacturing cost is reduced. In addition, the two-way valve is generally made of a transparent material such as plastic or glass, and the sealed space in which the first container 11, the two-way valve, and the second container 12 communicate is a transparent passage, thereby improving convenience in observing the separation process of the PRP.
Optionally, the switch member 13 further has a vent end 131, the switch member 13 can be switched in at least three positions, in a first switching position, the first and second containers 11 and 12 are in communication via the passage in the switch member 13 and the vent end 131 is in communication with the first and second containers 11 and 12, respectively, is both disconnected by the switch member 13, in a second switching position, the communication between the first and second containers 11 and 12 and the communication between the vent end 131 and the first and second containers 11 and 12, respectively, are both disconnected by the switch member 13, and in a third switching position, the communication between the first container 11 and the vent end 131 and the second container 12, respectively, is disconnected by the switch member 13 and the vent end 131 is in communication with the second container 12 via the passage in the switch member 13.
Specifically, the transfer of the contents between the first container 11 and the second container 12 at the first switching position and the second switching position is similar to the transfer of the contents between the first container 11 and the second container 12 at the aforementioned first switching position and the second switching position. In the third switching position, the second regulating member slides away from the second communication port 124 under the drive of an external force, the volume of the second chamber 123 increases, and air in the external environment is drawn through the vent end 131 and the passage in the shutter 13 to further draw the content remaining in the passage of the shutter 13, such as PRP, into the second chamber 123.
Alternatively, the switch 13 may be configured as a three-way valve for enabling communication and disconnection between the first chamber 113 of the first container 11 and the second chamber 123 of the second container 12 and communication and disconnection between the venting end 131 and the first chamber 113 of the first container 11 and the second chamber 123 of the second container 12, respectively. Wherein the three-way valve may further be configured as a manually operated three-way valve comprising a three-way valve spool provided with a passage and a rotary piston controlling the rotation of the three-way valve spool, the rotary piston being positioned in a first switching position by operation of the rotary piston such that the passage in the three-way valve spool is aligned with the first and second ends of the three-way valve to align the first and second communication ports 114, 124 and to close off the third end of the three-way valve, thereby bringing the first and second containers 11, 12 into communication via the passage in the three-way valve spool and disconnecting the venting end 131 from the first and second containers 11, 12, respectively. Or the rotary piston is positioned at the second switching position, so that the three-way valve core blocks the first end, the second end and the third end of the three-way valve, thereby disconnecting the first container 11, the second container 12 and the ventilation end 131. Or the rotary piston is positioned in a third switching position so that the passage in the three-way valve core is aligned with the second end and the third end to close off the first end, thereby allowing communication between the venting end 131 and the second container 12 and disconnecting the first container 11 from the venting end 131 and the second container 12, respectively. In practical application, the three-way valve can also be in other conventional three-way valve structures. The three-way valve has wide application in the field of medical instruments, simple and convenient structure operation and low price, thereby greatly reducing the operation difficulty of the extracting device 1 for extracting platelet-rich plasma from blood and reducing the manufacturing cost.
Optionally, the free end of the venting end 131 may be provided with a sterilizing filter membrane to ensure a sterile environment inside the extraction device 1 for extracting platelet rich plasma from blood in this embodiment and to reduce the risk of introducing contamination during the extraction of outside air by the second container 12. In addition, a first sealing member 132, such as a sealing cap or a sealing plug, can be detachably connected to the free end of the ventilation end 131 to further improve the sealing performance of the extraction device 1 for extracting platelet-rich plasma from blood in the present embodiment.
Alternatively, the switch member 13 is provided with a first locking member corresponding to an end connecting the first communicating port 114, and the first communicating port 114 is provided with a first mating locking member cooperating with the first locking member to lock the switch member 13 and the first container 11. The one end that switch piece 13 corresponds and connects second intercommunication mouth 124 is equipped with the second locking piece, and second intercommunication mouth 124 is equipped with and cooperatees with the second locking piece in order to lock the second of switch piece 13 and second container 12 and mate the locking piece.
Wherein, the first locking member, the first mating locking member, the second locking member and the second mating locking member can be made of transparent material such as glass or plastic, so that the sealing performance can be maintained while the locking between the switch member 13 and the first container 11 and the second container 12 is ensured, and the convenience of observing and controlling the PRP extraction process can be further improved. In addition, a sealing ring, such as a rubber ring, may be separately provided at a joint of the first locking member and the first mating locking member or a joint of the second locking member and the second mating locking member to further improve sealing performance.
Optionally, the first container 11 is configured such that when the switch member 13 is removed, the free end of the first mating latch member has a second seal member removably attached thereto. The second container 12 is configured such that when the switch member 13 is removed, the free end of the second mating latch member has a third seal member removably attached thereto.
Alternatively, the connection between the first locking means and the first counter locking means and the connection between the second locking means and the second counter locking means can be designed as a screw thread and/or snap-fit engagement, i.e. the connection between the first locking means and the first counter locking means and the connection between the second locking means and the second counter locking means can be identical or different.
Illustratively, when the connection means is designed to be screw-engaged, the first locking member may be an internal/external screw thread provided on an end of the opening/closing member 13 corresponding to the first communication port 114, and the first counterpart locking member may be an external/internal screw thread provided on the first communication port 114. Alternatively, the first and second counterpart locking members may be separate screw-threaded connection members to connect the switching member 13 and the first container 11, for example, the first locking member may be configured as one of a male luer connector having an external thread/a female luer connector having an internal thread, and the first counterpart locking member may be configured as the other. The second latch and the second mating latch may be similar to the first latch and the first mating latch. Further, the second seal may be configured as a threaded plug or cap that is threadably engaged with the first mating lock, and the third seal may be the same as or different from the second seal. In practical application, the first locking member and the first mating locking member and the second mating locking member may be other detachable non-permeable structures. In addition, when the first container 11 is configured to be detached from the switch member 13, the free end of the first mating locking member is detachably connected to the needle assembly.
Alternatively, the content in the second chamber 123 is a part of the content in the first chamber 113 in terms of volume, and the maximum volume of the first chamber 113 may be larger than the maximum volume of the second chamber 123, so as to further reduce the manufacturing cost of the extraction device 1 for extracting platelet-rich plasma from blood in the present embodiment. Generally, the PRP preparation amount is about 10% of the amount of collected blood, and thus, for example, the first container 11 may require a volume of 1-100mL, and the second container 12 may require a volume of 1-10 mL. More specifically, if the volume required for taking blood is 100mL, the volume required for taking PPP and PRP is 55mL, and the volume required for taking PRP is 10mL, and accordingly, the first container 11 and the second container 12 can be selected according to the actual volumes contained therein.
The following will describe a method and process for extracting PRP from blood using the above-described device, as shown in fig. 1 and 4. During preparation, a needle assembly may be mounted on the first communication port 114 of the first container 11 to collect blood. During extraction, the first communication port 114 of the first container 11 containing the collected blood may be first placed upward and the attached needle assembly detached to attach a second seal, and the sealed first container 11 may be secured in a centrifuge tube designed to match the shape and size of the first container 11 for centrifugation. The contents of the first chamber 113 are divided into a PPP layer, a PRP layer, and a red blood cell layer from top to bottom. Next, the second seal on the first container 11 and the third seal on the second container 12 are removed to sealingly connect the first container 11 and the second container 12 to the switch 13, and the switch 13 is switched to the first switching position, pushing the first regulating member 112 to transfer the PPP layer in the first chamber 113 to the second chamber 123 of the second container 12 without contents via the channel in the switch 13, with the PRP layer and the red blood cell layer still in the first chamber 113. Then, the second container 12 containing the PPP layer is detached from the switch member 13, and replaced with a new second container 12 containing no contents and hermetically connected to the switch member 13, and the first regulating member 112 is continuously pushed to transfer the PRP layer in the first chamber 113 into the replaced second container 12. Alternatively, the shutter 13 may be switched to a third switching position, pulling the second shutter to draw air to draw the remaining PRP in the shutter 13 into the second chamber 123.
During this centrifugation movement, the first container 11 containing blood and a syringe containing water or blood and having the same size as the first container 11 are fixed in the centrifuge tube of the centrifuge from which the separation rod has been removed to achieve syringe balancing. Then centrifugal movement is carried out under the condition of the rotation speed of 1000-. It should be noted that after the first container 11 is centrifugally moved, the first communication port 114 of the first container 11 is kept in a constant orientation.
In another embodiment, a needle assembly may be mounted on the first communication port 114 of the first container 11 to collect blood during preparation. During extraction, the first communication port 114 of the first container 11 containing the collected blood may first be placed down and the attached needle assembly detached to attach a second seal, and the sealed first container 11 may be secured in a centrifuge tube designed to match the shape and size of the first container 11 for centrifugation. The contents of the first chamber 113 are divided into a PPP layer, a PRP layer, and a red blood cell layer from top to bottom. Next, the second seal on the first container 11 and the third seal on the second container 12 are removed to sealingly connect the first container 11 and the second container 12 to the switch 13, and the switch 13 is switched to the first switching position, pushing the first regulating member 112 to transfer the layer of red blood cells in the first chamber 113 to the second chamber 123 of the second container 12 without contents via the channel in the switch 13, with the PPP layer and the PRP layer still located in the first chamber 113. The second container 12 containing the layer of red blood cells is then removed from the switch member 13 and replaced with a new second container 12 containing no contents sealingly attached to the switch member 13, and the first adjustment member 112 is pushed on to transfer the PRP layer in the first chamber 113 into the replaced second container 12. Alternatively, the shutter 13 may be switched to a third switching position, pulling the second shutter to draw air to draw the remaining PRP in the shutter 13 into the second chamber 123.
It should be noted that after the first container 11 is centrifugally moved, it is ensured that the first communication port 114 of the first container 11 is kept facing downward.
In another embodiment, as shown in fig. 1 to 4, the first communication port 114 of the first container 11 containing the collected blood may be first placed upward and the second sealing member may be installed, the sealed first container 11 is fixed in a centrifuge tube in a centrifuge for a first centrifugation motion at 2500 rpm for 10 minutes, and the contents in the first chamber 113 are separated into PPP layer, PRP layer and red blood cell layer from top to bottom. Next, removing the second seal on the first container 11 and the third seal on the second container 12 to sealingly connect the first container 11 and the second container 12 to the switch 13 and cause the switch 13 to be switched to the first switching position, pushing the first regulating member 112 to transfer the PPP layer and the PRP layer in the first chamber 113 to the second chamber 123 of the second container 12 without contents via the channel in the switch 13, with the red blood cell layer still in the first chamber 113. Then, the shutter 13 is switched to the third switching position, and the second shutter is pulled to draw air to draw the PRP remaining in the shutter 13 into the second chamber 123. In addition, the switch member 13 is switched to the second switching position and the second container 12 is detached from the switch member 13, so that the second communication port 124 of the second container 12 is placed upward and the third sealing member is mounted for the second centrifugal movement, the rotating speed can be 3300 rpm, the centrifugal time can be 8 minutes, and the content in the second chamber 123 is divided into the PPP layer and the PRP layer from top to bottom. The third sealing member of the second container 12 is removed and the switch member 13 is rotated and then connected to the switch member 13, so that the switch member 13 is switched to the first switching position, and the second regulating member is pushed to transfer the PPP layer in the second chamber 123 into the first container 11, so that the PRP layer is only contained in the second container 12 and the PRP is extracted from the blood contained in the first container 11. Finally, the second container 12 can be removed from the switch member 13 and a third seal can be installed.
After the first centrifugal movement of the first container 11 and the second centrifugal movement of the second container 12, it is necessary to ensure that the orientation of the first communication port 114 of the first container 11 is not changed and the orientation of the second communication port 124 of the second container 12 is not changed by rotating the opening/closing member 13. Preferably, the centrifuge is operated at a higher speed during the second centrifugation than during the first centrifugation to improve the extraction quality of the PRP.
In another embodiment, the first communication port 114 of the first container 11 containing the collected blood may be first placed downward and the second sealing member may be installed, the sealed first container 11 may be fixed in a centrifuge tube in a centrifuge for a first centrifugation motion at 2500 rpm for 10 minutes, and the contents in the first chamber 113 may be separated into a PPP layer, a PRP layer and a PPP layer from top to bottom. Next, removing the second seal on the first container 11 and the third seal on the second container 12 sealingly connects the first container 11 and the second container 12 to the switch 13 and causes the switch 13 to be switched to the first switching position, pushing the first regulating member 112 to transfer the layer of red blood cells in the first chamber 113 into the second chamber 123 of the second container 12 without contents via the channel in the switch 13, with the PPP layer and the PRP layer still located in the first chamber 113. Then, the shutter 13 is switched to the third switching position, and the first regulating member 112 is pulled to draw air to draw the PRP remaining in the shutter 13 into the first chamber 113. In addition, the switch member 13 is switched to the second switching position and the first container 11 and the second container 12 are detached from the switch member 13, so that the first communication port 114 of the first container 11 is placed downward and the second sealing member is mounted for the second centrifugal movement, the rotating speed can be 3300 rpm, the centrifugal time can be 8 minutes, and the content in the first chamber 113 is divided into the PPP layer and the PRP layer from top to bottom. The second sealing member of the first container 11 is removed and the switching member 13 is rotated to be hermetically connected to the switching member 13, the second container 12 containing the red blood cell layer is replaced with a new second container 12 without contents and is connected to the switching member 13, the switching member 13 is switched to the first switching position, the first adjusting member 112 is pushed to transfer the PRP layer in the first chamber 113 into the replaced second container 12, so that the second container 12 contains only the PRP layer to extract PRP from the blood contained in the first container 11. Finally, the second container 12 containing the PRP can be removed from the switch member 13 and a third seal can be installed.
After the first centrifugation and the second centrifugation of the first container 11, the first communication port 114 of the first container 11 is kept facing downward.
In another embodiment, as shown in fig. 1 to 4, the first communication port 114 of the first container 11 containing the collected blood may be first placed downward and a second sealing member is installed, the sealed first container 11 is fixed in a centrifuge tube in a centrifuge for a first centrifugation movement, and the content in the first chamber 113 is divided into a PPP layer, a PRP layer and a red blood cell layer from top to bottom, wherein the red blood cell layer is closer to the first communication port 114. Next, removing the second seal on the first container 11 and the third seal on the second container 12 connects the first container 11 and the second container 12 to the switch 13 and causes the switch 13 to be switched to the first switching position, pushing the first regulating member 112 to transfer the layer of red blood cells in the first chamber 113 into the second chamber 123 of the second container 12 without contents via the channel in the switch 13, with the PPP layer and the PRP layer still located in the first chamber 113. Then, the opening/closing member 13 is switched to the second switching position and the first container 11 is detached from the opening/closing member 13, the first communication port 114 of the first container 12 is placed upward and the second sealing member is mounted for the second centrifugal movement, and the content in the first chamber 113 is divided into a PPP layer and a PRP layer from top to bottom, wherein the PPP layer is closer to the first communication port 114. The second seal of the first container 11 is removed and the switch 13 is rotated and then connected to the switch 13, so that the switch 13 is switched to the first switching position, and the first adjusting member 112 is pushed to transfer the PPP layer in the first chamber 113 to the second container 12, so that the first container 11 only contains the PRP layer and the PRP is extracted from the blood initially contained in the first container 11. Finally, the first container 11 can be removed from the switch member 13 and a second seal can be installed.
After the first container 11 is centrifuged for the first time, the first communication port 114 is kept downward, and after the first container 11 is centrifuged for the second time, the first communication port 114 is kept upward by rotating the switch 13.
In another embodiment, the first communication port 114 of the first container 11 containing the collected blood may be first placed upward and a second sealing member may be installed, the sealed first container 11 is fixed in a centrifuge tube in a centrifuge for a first centrifugation movement, and the contents in the first chamber 113 are divided into a PPP layer, a PRP layer and a red blood cell layer from top to bottom, wherein the PPP layer is closer to the first communication port 114. Next, removing the second seal on the first container 11 and the third seal on the second container 12 connects the first container 11 and the second container 12 to the switch 13 and causes the switch 13 to be switched to the first switching position, pushing the first regulating member 112 to transfer the PPP layer and the PRP layer in the first chamber 113 to the second chamber 123 of the second container 12 without contents via the channel in the switch 13, the red blood cell layer being located in the first chamber 113. Then, the switch member 13 is switched to the second switching position and the second container 12 is detached from the switch member 13, so that the second communication port 124 of the second container 12 is placed downward and the third sealing member is mounted for the second centrifugal movement, and the content in the second chamber 123 is divided into a PPP layer and a PRP layer from top to bottom, wherein the PRP layer is closer to the second communication port 124. The first container 11 is replaced with a new first container 11 containing no contents and hermetically connected to the switch 13, the third sealing member of the second container 12 is removed and the switch 13 is rotated and then connected to the switch 13, so that the switch 13 is switched to the first switching position, and the second regulating member is pushed to transfer the PRP layer in the second chamber 123 into the replaced first container 11, so that the replaced first container 11 contains only the PRP layer to extract PRP from the blood contained in the first container 11 originally containing blood. Finally, the first container 11 can be removed from the switch member 13 and a second seal can be installed.
It is necessary to ensure that the first communication port 114 is kept upward after the first centrifugal motion of the first container 11, and that the second communication port 124 is kept downward by rotating the opening/closing member 13 after the second centrifugal motion of the second container 12.
It should be understood that any combination of the operation steps of the extraction device in this embodiment to extract PRP should fall within the protection scope of the present invention.
It should be understood that although the description is in terms of various embodiments, not every embodiment includes only a single embodiment, and such description is for clarity purposes only, and those skilled in the art will recognize that the embodiments described herein may be combined as suitable to form other embodiments, as will be appreciated by those skilled in the art.
The above description is only an exemplary embodiment of the present invention, and is not intended to limit the scope of the present invention. Any equivalent alterations, modifications and combinations can be made by those skilled in the art without departing from the spirit and principles of the invention.
Claims (16)
1. An extraction device for extracting platelet rich plasma from blood, comprising:
the first container comprises a first outer wall, a first adjusting piece sliding along the inner side of the first outer wall in a sealing mode, a first chamber defined by the first outer wall and the first adjusting piece, and a first communication port penetrating through the first outer wall and communicated with the first chamber;
the second container comprises a second outer wall, a sealing end piece arranged on the second outer wall, a second chamber enclosed by the second outer wall and the sealing end piece, and a second communication port penetrating through the second outer wall and communicated with the second chamber;
the two ends of the switch piece are respectively detachably and hermetically connected with the first communicating port of the first container and the second communicating port of the second container, and a channel is arranged in the switch piece;
wherein the switch member is switchable between at least two positions, a first switching position in which the first and second containers are in communication via the passageway in the switch member and the contents of the first chamber are transferred to the second chamber under sliding movement of the first adjustment member to extract platelet rich plasma from the blood contained in the first container, and a second switching position in which communication between the first and second containers is interrupted by the switch member.
2. The device of claim 1, wherein the sealing member is configured to sealingly slide along an inner side of the second outer wall, such that in the first switching position, contents in the first chamber and contents in the second chamber are displaced from each other by sliding movement of the first and second members.
3. The extraction device of platelet rich plasma from blood as claimed in claim 1, wherein said switch member is configured as a two-way valve.
4. An extraction device for extracting platelet rich plasma from blood as claimed in claim 2, wherein the first container and/or the second container is configured as a syringe.
5. The device according to claim 2, wherein the switch member further has a vent end, and the switch member is switchable between at least three positions, wherein in a first switching position, the first and second containers are in communication via the passage in the switch member and the communication between the vent end and the second container is interrupted by the switch member, in a second switching position, the communication between the first and second containers and the communication between the vent end and the second container are both interrupted by the switch member, and in a third switching position, the communication between the first and second containers is interrupted by the switch member and the vent end is in communication with the second container via the passage in the switch member.
6. The device of claim 5, wherein the switch is configured as a three-way valve.
7. The device of claim 5, wherein the first sealing member is removably attached to the free end of the vent end.
8. The extraction apparatus for extracting platelet-rich plasma from blood according to claim 1,
the first connecting port is provided with a first locking piece, and the first connecting port is provided with a first matching locking piece which is matched with the first locking piece to lock the switch piece and the first container;
the switch piece corresponds the one end of connecting the second intercommunication mouth and is equipped with the second locking piece, the second intercommunication mouth be equipped with the cooperation of second locking piece is in order to lock the switch piece with the second of second container pairs the locking piece.
9. The extraction apparatus for extracting platelet-rich plasma from blood according to claim 8,
the first lock is configured as a luer male/female connector, and the first mating lock is configured as a luer female/luer male connector;
the second locking member is configured as a luer male/female connector and the second mating locking member is configured as a luer female/luer male connector.
10. The extraction apparatus for extracting platelet-rich plasma from blood according to claim 8,
the first container is configured such that when the switch member is removed, a second sealing member is removably attached to a free end of the first mating locking member;
the second container is configured such that when the switch member is removed, a third sealing member is removably attached to a free end of the second mating locking member.
11. An extraction device for extracting platelet rich plasma from blood as claimed in claim 8, wherein the connection between the first lock and the first mating lock and the connection between the second lock and the second mating lock are designed to be in a threaded and/or snap engagement.
12. The device of claim 8, wherein the first and/or second container, the first and/or second mating latch, and the second and/or third mating latch are constructed of a transparent material.
13. A method for extracting platelet rich plasma from blood using the extraction device for extracting platelet rich plasma from blood according to any one of claims 1 to 12, the method comprising:
placing the first communication port of the first container containing the collected blood upward for first centrifugation, wherein the contents in the first chamber are divided into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom;
sealingly connecting the first and second containers to the switch and causing the switch to be switched in a first switching position, pushing the first regulating member to transfer the platelet poor plasma layer in the first chamber into the second chamber free of contents of the second container via the channel in the switch;
switching the switch member to a second switching position and detaching a second container containing a platelet poor plasma layer from the switch member;
and replacing the second container containing the platelet-poor plasma layer with a new second container containing no content, connecting the new second container containing no content to the switch member in a sealing manner, switching the switch member to a first switching position, pushing the first adjusting member to push the platelet-rich plasma layer in the first chamber into the new second container containing no content, and extracting the platelet-rich plasma from the blood contained in the first container by only containing the platelet-rich plasma layer in the new second container containing no content.
14. A method for extracting platelet rich plasma from blood using the extraction device for extracting platelet rich plasma from blood according to any one of claims 2 to 12, the method comprising:
placing the first communication port of the first container containing the collected blood upward for first centrifugation, wherein the contents in the first chamber are divided into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom;
sealingly connecting the first and second containers to the switch and causing the switch to be switched to a first switch position, pushing the first regulator to transfer the platelet and platelet rich plasma layers in the first chamber into the second chamber free of contents via the channel in the switch;
switching the switch piece to a second switching position and detaching the second container from the switch piece, so that the second communication port of the second container is placed upwards for second centrifugation, and the content in the second chamber is divided into a platelet poor plasma layer and a platelet rich plasma layer from top to bottom;
and the second container is hermetically connected to the switch member, the switch member is switched to the first switching position, and the second adjusting member is pushed to transfer the platelet poor plasma layer in the second cavity into the first container, so that the second container only contains the platelet rich plasma layer to extract the platelet rich plasma from the blood contained in the first container.
15. The method of claim 14, wherein prior to switching the switch member to the second switch position and removing the second container from the switch member, comprises:
switching the switch member to a third switching position, and pulling the second regulating member to draw air through the venting end to draw platelet rich plasma remaining in the switch member into the second chamber.
16. A method for extracting platelet rich plasma from blood using the extraction device for extracting platelet rich plasma from blood according to any one of claims 2 to 12, the method comprising:
placing the first container containing the collected blood with the first communication port facing downward for a first centrifugation, wherein the contents in the first chamber are divided into a platelet poor plasma layer, a platelet rich plasma layer and a red blood cell layer from top to bottom;
sealingly connecting the first and second containers to the switch and causing the switch to be switched in a first switching position, urging the first regulating member to transfer the layer of red blood cells in the first chamber into the second chamber free of contents of the second container via the channel in the switch;
switching the switch piece to a second switching position and detaching the first container from the switch piece, so that the first communication port of the first container is placed upwards for second centrifugation, and the content in the first chamber is divided into a platelet poor plasma layer and a platelet rich plasma layer from top to bottom;
and the first container is hermetically connected to the switch member, the switch member is switched to a first switching position, and the first adjusting member is pushed to transfer the platelet poor plasma layer in the first cavity into the second container, so that the first container only contains the platelet rich plasma layer to extract the platelet rich plasma from the blood contained in the first container.
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| CN201910987018.8A CN112675577A (en) | 2019-10-17 | 2019-10-17 | Extraction device and method for extracting platelet-rich plasma from blood |
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| CN201910987018.8A CN112675577A (en) | 2019-10-17 | 2019-10-17 | Extraction device and method for extracting platelet-rich plasma from blood |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113546450A (en) * | 2021-07-27 | 2021-10-26 | 伯格曼(江苏)医疗科技有限公司 | Multipurpose platelet and plasma preparation device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113546450A (en) * | 2021-07-27 | 2021-10-26 | 伯格曼(江苏)医疗科技有限公司 | Multipurpose platelet and plasma preparation device |
| CN113546450B (en) * | 2021-07-27 | 2022-06-28 | 伯格曼(江苏)医疗科技有限公司 | Multipurpose platelet and plasma preparation device |
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