CN111036421B - Combined type disposable centrifuge tube, centrifugal device and PRP preparation method - Google Patents
Combined type disposable centrifuge tube, centrifugal device and PRP preparation method Download PDFInfo
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- CN111036421B CN111036421B CN201911250424.2A CN201911250424A CN111036421B CN 111036421 B CN111036421 B CN 111036421B CN 201911250424 A CN201911250424 A CN 201911250424A CN 111036421 B CN111036421 B CN 111036421B
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- 238000002360 preparation method Methods 0.000 title claims description 8
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 claims abstract description 6
- 238000005192 partition Methods 0.000 claims description 36
- 210000004369 blood Anatomy 0.000 claims description 26
- 239000008280 blood Substances 0.000 claims description 26
- 238000002347 injection Methods 0.000 claims description 16
- 239000007924 injection Substances 0.000 claims description 16
- 238000007789 sealing Methods 0.000 claims description 8
- 230000001681 protective effect Effects 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 230000009471 action Effects 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 210000001772 blood platelet Anatomy 0.000 description 11
- 238000005119 centrifugation Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 3
- 238000010241 blood sampling Methods 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 210000004872 soft tissue Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011982 device technology Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- External Artificial Organs (AREA)
- Centrifugal Separators (AREA)
Abstract
The present disclosure provides a modular, disposable centrifuge tube and centrifuge device, PRP manufacturing method. The centrifuge tube comprises a first bin body with two open ends, a second bin body with two open ends and a third bin body with two open ends, wherein the first bin body, the second bin body and the third bin body are sequentially and detachably connected through a luer bi-pass joint with a valve, and the valve can be opened or closed; wherein, be provided with the division board in the third storehouse body, the division board can be followed the direction of height of the third storehouse body and slided, and the one end that the second storehouse body was kept away from to the third storehouse body is provided with the adjusting device who is connected with the division board, can drive the division board through adjusting device and slide along the direction of height of the third storehouse body to adjust the height position of division board in the third storehouse body. The present disclosure can ensure that the extracted platelets are of high purity and are not contaminated with erythrocytes when necessary.
Description
Technical Field
Embodiments of the present disclosure relate generally to the field of medical device technology, and more particularly, to a modular single use centrifuge tube and centrifuge device, PRP manufacturing method.
Background
The existing centrifuge tube is of an integral structure, when blood layered after centrifugal treatment is extracted, a platelet layer and a erythrocyte layer are located in the same centrifuge tube, and erythrocytes are mixed in the process of extracting platelets, so that the purity of the extracted platelets is insufficient, the results of subsequent tests and detection are affected, and further the clinical curative effect is affected.
Disclosure of Invention
In a first aspect of the present disclosure, a disposable PRP manufacturing centrifuge tube is provided, including a first bin body with two open ends, a second bin body with two open ends, and a third bin body with two open ends, where the first bin body, the second bin body, and the third bin body are detachably connected in sequence through a luer two-way connector with a valve, and the valve can be opened or closed; the third bin is internally provided with a partition plate, the third bin is internally provided with a first cavity and a second cavity which are separated by the partition plate, the first cavity is positioned at one side of the partition plate, which is close to the second bin, and can be communicated with the second bin, the second cavity is positioned at one side of the partition plate, which is far away from the second bin, and the partition plate can slide along the height direction of the third bin so as to adjust the volume of the first cavity and the second cavity, one end of the third bin, which is far away from the second bin, is provided with an adjusting device connected with the partition plate, and the partition plate can be driven to slide along the height direction of the third bin through the adjusting device so as to adjust the height position of the partition plate in the third bin.
As described above, in one possible implementation manner, there is further provided an implementation manner, where the adjusting device includes a connecting rod and an adjusting cap, where the adjusting cap is sleeved on an outer sidewall of the third bin body and is capable of moving along a height direction of the third bin body, and the connecting rod is respectively connected with the partition plate and the adjusting cap, and is capable of driving the partition plate to slide along the height direction of the third bin body under the action of the adjusting cap.
In the aspect and any possible implementation manner described above, there is further provided an implementation manner, an external thread is provided on an outer side wall of the third bin body, an internal thread matched with the external thread is provided on the adjusting cap, and the adjusting cap is installed on the outer side wall of the third bin body through the matching screwing of the internal thread and the external thread.
As described above, in one possible implementation manner, there is further provided an implementation manner, one end of the first bin body connected with the second bin body is a closing-in structure, two ends of the second bin body are both closing-in structures, and one end of the third bin body connected with the second bin body is a closing-in structure.
In the aspect and any possible implementation manner as described above, there is further provided an implementation manner, where the closing-in structure is an inclined structure, and an inclination angle of the inclined structure is 45 ° to 60 °.
In the aspect and any possible implementation manner described above, there is further provided an implementation manner, an injection hole assembly is disposed at the closing-up structure of the third bin body, and one end of the injection hole assembly, which is far away from the outer surface of the third bin body, does not exceed a plane where a side wall of the third bin body is located.
In the aspect and any possible implementation manner described above, there is further provided an implementation manner, a middle portion of an end face of the first bin body, which is far away from one end of the second bin body, is provided with an opening.
Aspects and any one of the possible implementations as described above, further providing an implementation, further including: the sealing plug and the ventilation plug can be mounted at the opening to seal the opening.
In a second aspect of the present disclosure, there is provided a modular single use centrifugation device comprising: a protective frame with one end closed and one end opened; the adjusting cover is arranged at the opening of the protecting frame and is connected with the opening end of the protecting frame in a sliding way; the modular single use centrifuge tube of any of the first aspects, the modular single use centrifuge tube disposed within the protective frame.
In a third aspect of the present disclosure, there is provided a method of preparing PRP based on the disposable set-up centrifuge tube of any one of the first aspects, comprising: injecting blood into the third bin body, and centrifuging the blood in the centrifuge tube; the height of the partition plate in the third bin body is adjusted to enable all or most of red blood cells at the lowest layer to be in the third bin body; closing a valve of the luer two-way joint between the second bin body and the third bin body, and disassembling the third bin body; carrying out centrifugal treatment on blood in the first bin body and the second bin body again; closing a valve of the luer two-way joint between the first bin body and the second bin body, and disassembling the first bin body to obtain the PRP in the second bin body.
In the assembled disposable centrifuge tube, the centrifugal device and the PRP preparation method provided by the embodiment of the disclosure, the first bin body, the second bin body and the third bin body which are detachably connected through the luer bi-pass connector form the centrifuge tube together, the third bin body is provided with the partition plate and the adjusting device, and the position of the partition plate in the third bin body is adjusted through the adjusting device, so that the blood platelets are positioned in the second bin body, and therefore, the purity of the blood platelets is high when the blood platelets are extracted, and red blood cells cannot be mixed when necessary.
It should be understood that what is described in this summary is not intended to limit the critical or essential features of the embodiments of the disclosure nor to limit the scope of the disclosure. Other features of the present disclosure will become apparent from the following description.
Drawings
The above and other features, advantages and aspects of embodiments of the present disclosure will become more apparent by reference to the following detailed description when taken in conjunction with the accompanying drawings. In the drawings, wherein like or similar reference numerals denote like or similar elements, in which:
FIG. 1 illustrates a schematic diagram of a modular single use centrifuge tube provided by an embodiment of the present disclosure;
FIG. 2 illustrates an assembly view of a modular single use centrifuge tube provided by an embodiment of the present disclosure;
fig. 3 shows a schematic structural view of a modular single-use centrifugal device provided by an embodiment of the present disclosure.
Wherein,
10. A first bin; 11. sealing the plugs; 12. a ventilation plug;
20. a second bin;
30. A third bin; 31. a partition plate; 32. a connecting rod; 33. an adjustment cap; 34. an injection hole assembly; 341. a first cap;
40. a luer bi-pass fitting; 401. a second blocking cap;
51. a protective frame; 52. and adjusting the cover.
Detailed Description
For the purposes of making the objects, technical solutions and advantages of the embodiments of the present disclosure more apparent, the technical solutions of the embodiments of the present disclosure will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present disclosure, and it is apparent that the described embodiments are some embodiments of the present disclosure, but not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments in this disclosure without inventive faculty, are intended to be within the scope of this disclosure.
In addition, the term "and/or" herein is merely an association relationship describing an association object, and means that three relationships may exist, for example, a and/or B may mean: a exists alone, A and B exist together, and B exists alone. In addition, the character "/" herein generally indicates that the front and rear associated objects are an "or" relationship.
Fig. 1 shows a schematic structural diagram of a assembled single-use centrifuge tube provided by an embodiment of the present disclosure, and fig. 2 shows an assembly drawing of the assembled single-use centrifuge tube provided by an embodiment of the present disclosure.
Referring to fig. 1 and 2, the centrifuge tube comprises a first bin body 10 with two open ends, a second bin body 20 with two open ends, and a third bin body 30 with two open ends, wherein the first bin body 10, the second bin body 20 and the third bin body 30 are sequentially and detachably connected through a luer double-pass connector 40 with a valve, and the valve can be opened or closed.
Be provided with division plate 31 in the third storehouse body 30, be provided with the first cavity and the second cavity that are separated by division plate 31 in the third storehouse body 30, first cavity is located the one side that division plate 31 is close to second storehouse body 20, and can communicate with second storehouse body 20, the second cavity is located the one side that division plate 31 kept away from second storehouse body 20, and division plate 31 can follow the direction of height of third storehouse body 30 and slide, in order to adjust the volume size of first cavity and second cavity, the one end that third storehouse body 30 kept away from second storehouse body 20 is provided with the adjusting device who is connected with division plate 31, can drive division plate 31 and slide along the direction of height of third storehouse body 30 through adjusting device, in order to adjust the height position of division plate 31 in third storehouse body 30.
The two bin bodies are connected by the luer double-way connector 40, so that one bin body can be disassembled without affecting the other bin bodies.
The partition plate 31 is in sealing connection with the inner side wall of the third bin 30, optionally the partition plate 31 is made of rubber material and is in interference fit connection with the inner side wall of the third bin 30.
In an example, from top to bottom, the first bin 10, the second bin 20 and the third bin 30 are coaxially arranged in sequence, the first bin 10 is used for storing a blood plasma layer after blood is centrifuged, the second bin 20 is used for storing a blood platelet layer after blood is centrifuged, and the third bin 30 is used for storing a red blood cell layer after blood is centrifuged.
When the device is specifically used, the valve of the luer two-way connector 40 is opened, blood is injected into the third bin body 30, the blood in the centrifuge tube is centrifuged, and the processed blood is divided into a plasma layer, a platelet layer and a red blood cell layer from top to bottom. When red blood cells need to be extracted, the height of the partition plate 31 in the third bin body 30 can be adjusted through the adjusting device, so that the volume of the first cavity is adjusted, after the height of the red blood cells reaches a proper position, the valve of the luer double-pass joint 40 between the third bin body 30 and the second bin body 20 is closed, all or most of red blood cells positioned at the lowest layer are positioned in the first cavity according to actual needs, the third bin body 30 is disassembled and discarded, the first bin body 10 and the second bin body 20 are left, the luer double-pass joint 40 between the third bin body 30 and the second bin body 20 is plugged by adopting the second plugging cap 401, and then secondary centrifugation is carried out.
The second cap 401 has a larger bottom area, and can prevent the pressure at the lower part of the luer double-pass connector 40 from being too high and damaging the components during the centrifugal movement.
When the platelets need to be extracted after the second centrifugation, the valve of the luer two-way connector 40 between the first cartridge body 10 and the second cartridge body 20 is closed, the first cartridge body 10 is discarded, and the second cartridge body 20 is reserved, wherein the required PRP is obtained.
In this embodiment, the first bin 10, the second bin 20 and the third bin 30 are detachably connected through the luer bi-pass connector 40 to form a centrifuge tube, the third bin 30 is provided with the partition plate 31 and the adjusting device, and the position of the partition plate 31 in the third bin 30 is adjusted through the adjusting device to enable the blood platelets to be located in the second bin 20, so that the purity of the blood platelets is high when PRP is extracted, and red blood cells are not mixed when necessary.
In this embodiment, the first cartridge body 10, the second cartridge body 20 and the third cartridge body 30 are sequentially connected to form a soft tissue type centrifuge tube or a bone tissue type centrifuge tube.
The total capacity of the soft tissue centrifuge tube can be 80ml, the corresponding blood sampling amount is 60-70 ml, the capacity of the first bin body 10 can be 35ml, the capacity of the second bin body 20 can be 10ml, and the maximum capacity of the third bin body 30 can be 45ml. The third cartridge body 30 may change its capacity by adjusting the position of the partition plate 31 therein.
The total capacity of the bone tissue centrifuge tube may be 140ml, the corresponding blood sampling amount may be 100-120 ml, the capacity of the first cartridge body 10 may be 60ml, the capacity of the second cartridge body 20 may be 13ml, and the maximum capacity of the third cartridge body 30 may be 70ml. The third cartridge body 30 may change its capacity by adjusting the position of the partition plate 31 therein.
In some embodiments, the adjusting device includes a connecting rod 32 and an adjusting cap 33, the adjusting cap 33 is sleeved on the outer side wall of the third bin 30 and can move along the height direction of the third bin 30, the connecting rod 32 is respectively connected with the partition plate 31 and the adjusting cap 33, and the partition plate 31 can be driven to slide along the height direction of the third bin 30 under the action of the adjusting cap 33. Further, an external thread is provided on the outer side wall of the third bin body 30, an internal thread matched with the external thread is provided on the adjusting cap 33, and the adjusting cap 33 is in threaded connection with the third bin body 30. When the position of the partition plate 31 in the third bin 30 needs to be adjusted, the adjusting cap 33 can be screwed, so that the depth of the adjusting rod 32 extending into the third bin 30 is changed, and the position of the partition plate 31 in the third bin 30 is changed, so that the volume of the third bin 30 is changed.
In this embodiment, the adjustment cap 33 and the third chamber 30 are connected by screw threads, so that the accuracy of the volume adjustment of the third chamber 30 is higher.
In some embodiments, to facilitate connection between the luer two-way connector 40 and three bin bodies, the first bin body 10, the second bin body 20 and the third bin body 30 are formed by a cylindrical portion and a conical-cylindrical-shaped closing-in structure, wherein one end of the first bin body 10 connected with the second bin body 20 is a closing-in structure, two ends of the second bin body 20 are both closing-in structures, one end of the third bin body 30 connected with the second bin body 20 is a closing-in structure, and the size of an end opening of the closing-in structure can be determined according to the size of an end opening of the luer two-way connector 40, so long as sealing connection between the first bin body 10, the second bin body 20 and the third bin body 30 and the luer two-way connector 40 is ensured.
Optionally, the closing-in structure is an inclined structure, that is, the side wall surface of the first bin 10, the second bin 20 or the third bin 30 is inclined towards the axis direction at the end of the side wall surface, and the inclination angle is 45-60 degrees. Preferably, the inclination angle of the closing-in structure is 45 °.
In this embodiment, through the setting of binding off structure, can be more favorable to with luer bi-pass connect 40 between be connected, also make the blood in the centrifuging tube flow into next storehouse in vivo more easily simultaneously.
In some embodiments, the third cartridge body 30 is provided with an injection hole assembly at its closing-in structure, optionally, the injection hole assembly is a cylinder connected to the outer surface of the third cartridge body 30 and parallel to the bottom wall of the third cartridge body 30, and the cylinder is hollow inside, forming a channel communicating with the internal cavity of the third cartridge body 30, from which channel blood is injected into the third cartridge body 30. Further, the end of the injection hole assembly far away from the outer surface of the third bin body 30 does not exceed the plane of the side wall of the cylindrical part of the third bin body 30, so that the fracture of the injection hole assembly caused by collision is avoided. In this embodiment, blood may be injected into the third cartridge body 30 through the injection port assembly.
Optionally, the centrifuge tube further comprises a first cap 341, the injection hole assembly 34 being sealed with the first cap 341 when the injection hole assembly 34 is not in use.
In some embodiments, the first cartridge body 10 has an opening in the middle of an end surface of the first cartridge body remote from the second cartridge body 20. Optionally, the centrifuging tube still include with opening matched sealing plug 11 and ventilative end cap 12, sealing plug 11 uses when centrifuging after the centrifuging tube is filled with blood, can seal the opening, and ventilative end cap 12 uses when filling blood into the centrifuging tube, is equipped with antibacterial dustproof filter membrane in the ventilative end cap 12, can guarantee that the atmospheric pressure in the centrifuging tube is the same with external atmospheric pressure in the centrifuging tube, also can prevent that bacterium in the air from entering into the centrifuging tube.
In another aspect, embodiments of the present disclosure also provide a modular single use centrifugation device, see fig. 3, that includes a protective frame 51, an adjustment cover 52, and a centrifuge tube as provided by the embodiments described above. Wherein, the one end of fender bracket 51 is sealed one end opening, and the centrifuging tube is placed in fender bracket 51, and the adjustment lid 52 lid dress is in the opening part of fender bracket 51, with fender bracket 51 open end sliding connection, through the position of adjustment lid 52 for the bottom of fender bracket 51 and adjustment lid 52 clamp together fixed centrifuging tube, have further strengthened the rigidity of centrifuging tube, make the centrifuging tube not fragile when centrifuging.
Alternatively, since the centrifuge tube is composed of the first bin 10, the second bin 20 and the third bin 30, it may be only necessary to centrifuge the centrifuge tube composed of the first bin 10 and the second bin 20 when in use, and thus the height of the protective frame 51 is set to be smaller than the height of the centrifuge tube.
In another aspect, embodiments of the present disclosure also provide a method of preparing PRP based on the centrifuge tube of the above embodiments.
Specifically, the method comprises the following steps:
Step S1, injecting blood into the third bin body 30, and centrifuging the blood in the centrifuge tube.
In this embodiment, when blood is injected into the third chamber body 30, the valve of the luer two-way connector 40 between the first chamber body 10 and the second chamber body 20 is in an open state, the valve of the luer two-way connector 40 between the second chamber body 20 and the third chamber body 30 is also in an open state, and the air-permeable plug 12 is adopted at the opening of the first chamber body 10, the injection is performed through the injection hole assembly 34 on the third chamber body 30, after the blood is injected, the first plug cap 341 is adopted to seal the injection hole assembly 34, and the air-permeable plug 12 is replaced by the sealing plug 11.
Alternatively, the centrifuge tube may be placed laterally and blood injected into the third cartridge body 30 through the injection port assembly 34.
In step S2, the height of the partition plate 31 in the third chamber 30 is adjusted so that all or most of the red blood cells located at the lowest layer are located in the third chamber 30.
In the blood after centrifugation, the red blood cells are located at the lowest layer, and all or most of the red blood cells located at the lowest layer can be located at the third chamber 30 by adjusting the height of the partition plate 31 in the third chamber 30. With the majority of the red blood cells located at the lowermost layer in the third cartridge body 30, for example, 4/5 of the volume of all red blood cells may be located in the third cartridge body 30.
And S3, closing a valve of the luer double-pass connector 40 between the second bin body 20 and the third bin body 30, and disassembling the third bin body 30.
Step S4, the blood in the first bin 10 and the second bin 20 is centrifuged again.
In this embodiment, a second cap 401 may be used to seal the luer duplex 40 between the second cartridge body 20 and the third cartridge body 30 during the re-centrifugation process.
And S5, closing a valve of the luer double-pass connector 40 between the first bin body 10 and the second bin body 20, and disassembling the first bin body 10 to obtain the PRP in the second bin body 20.
The second centrifugation of the blood in the first cartridge body 10 and the second cartridge body 20 can cause the PRP to be entirely located in the second cartridge body 20.
In the description of the present specification, the terms "connected," "mounted," "secured," and the like are to be construed broadly, and for example, "connected" may be a fixed connection, a removable connection, or an integral connection; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.
In the description of the present specification, the terms "one embodiment," "some embodiments," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above is only a preferred embodiment of the present application, and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (9)
1. The PRP preparation method based on the assembled disposable centrifuge tube is used for preparing the PRP and is characterized in that the assembled disposable centrifuge tube comprises a first bin body with two openings, a second bin body with two openings and a third bin body with two openings, wherein the first bin body, the second bin body and the third bin body are sequentially and detachably connected through a luer double-pass connector with a valve, the valve can be opened or closed, the bin bodies are connected through the luer double-pass connector, and one bin body can be detached under the condition that the rest bin bodies are not affected;
Wherein the third bin body is internally provided with a partition plate, the third bin body is internally provided with a first cavity and a second cavity which are separated by the partition plate, the first cavity is positioned at one side of the partition plate, which is close to the second bin body, and can be communicated with the second bin body, the second cavity is positioned at one side of the partition plate, which is far away from the second bin body, and the partition plate can slide along the height direction of the third bin body so as to adjust the volume of the first cavity and the second cavity, one end of the third bin body, which is far away from the second bin body, is provided with an adjusting device connected with the partition plate, the partition plate can be driven to slide along the height direction of the third bin body through the adjusting device so as to adjust the height position of the partition plate in the third bin body,
When PRP is prepared by a modular single use centrifuge tube,
Injecting blood into the third bin body, and centrifuging the blood in the centrifuge tube;
the height of the partition plate in the third bin body is adjusted to enable all or most of red blood cells at the lowest layer to be in the third bin body;
closing a valve of the luer two-way joint between the second bin body and the third bin body, and disassembling the third bin body;
carrying out centrifugal treatment on blood in the first bin body and the second bin body again;
closing a valve of the luer two-way joint between the first bin body and the second bin body, and disassembling the first bin body to obtain the PRP in the second bin body.
2. The PRP preparation method based on the assembled type disposable centrifuge tube according to claim 1, wherein the adjusting device comprises a connecting rod and an adjusting cap, the adjusting cap is sleeved on the outer side wall of the third bin body and can move along the height direction of the third bin body, the connecting rod is respectively connected with the separating plate and the adjusting cap, and the separating plate can be driven to slide along the height direction of the third bin body under the action of the adjusting cap.
3. The PRP manufacturing method based on the assembled single-use centrifuge tube according to claim 2, wherein an external thread is arranged on the outer side wall of the third bin body, an internal thread matched with the external thread is arranged on the adjusting cap, and the adjusting cap is installed on the outer side wall of the third bin body through the matching screwing of the internal thread and the external thread.
4. The PRP preparation method based on the assembled type single-use centrifugal tube, which is disclosed in claim 1, is characterized in that one end of the first bin body connected with the second bin body is of a closing-in structure, both ends of the second bin body are of closing-in structures, and one end of the third bin body connected with the second bin body is of a closing-in structure.
5. The PRP manufacturing method based on a combined type single-use centrifugal tube according to claim 4, wherein the closing-in structure is an inclined structure, and the inclination angle is 45-60 degrees.
6. The PRP manufacturing method based on the assembled single-use centrifugal tube according to claim 1, wherein an injection hole assembly is arranged at the closing-in structure of the third bin body, and one end of the injection hole assembly, which is far away from the outer surface of the third bin body, does not exceed the plane of the side wall of the third bin body.
7. The method for preparing PRP based on assembled single-use centrifuge tube according to claim 1, wherein the middle part of the end surface of the first bin body far away from the end of the second bin body is provided with an opening.
8. The method for preparing PRP based on a modular single use centrifuge tube of claim 7, further comprising:
the sealing plug and the ventilation plug can be mounted at the opening to seal the opening.
9. A PRP preparation method based on a modular single-use centrifugal device for preparing PRP, comprising:
A protective frame with one end closed and one end opened;
the adjusting cover is arranged at the opening of the protecting frame and is connected with the opening end of the protecting frame in a sliding way;
The modular single use centrifuge tube of any one of claims 1 to 8 disposed within the protective frame for preparing PRP by the PRP preparation method of any one of claims 1 to 8.
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| CN201911250424.2A CN111036421B (en) | 2019-12-09 | 2019-12-09 | Combined type disposable centrifuge tube, centrifugal device and PRP preparation method |
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|---|---|---|---|
| CN201911250424.2A CN111036421B (en) | 2019-12-09 | 2019-12-09 | Combined type disposable centrifuge tube, centrifugal device and PRP preparation method |
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| CN111036421B true CN111036421B (en) | 2024-05-17 |
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| CN111036421A (en) | 2020-04-21 |
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