CN111036421A - Assembled disposable centrifugal tube, centrifugal device and PRP preparation method - Google Patents
Assembled disposable centrifugal tube, centrifugal device and PRP preparation method Download PDFInfo
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- CN111036421A CN111036421A CN201911250424.2A CN201911250424A CN111036421A CN 111036421 A CN111036421 A CN 111036421A CN 201911250424 A CN201911250424 A CN 201911250424A CN 111036421 A CN111036421 A CN 111036421A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 18
- 210000004369 blood Anatomy 0.000 claims description 27
- 239000008280 blood Substances 0.000 claims description 27
- 238000000926 separation method Methods 0.000 claims description 27
- 238000005192 partition Methods 0.000 claims description 17
- 238000002347 injection Methods 0.000 claims description 15
- 239000007924 injection Substances 0.000 claims description 15
- 238000005119 centrifugation Methods 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 3
- 230000001681 protective effect Effects 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims description 2
- 210000001772 blood platelet Anatomy 0.000 abstract description 13
- 230000008859 change Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 210000004872 soft tissue Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011982 device technology Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- External Artificial Organs (AREA)
- Centrifugal Separators (AREA)
Abstract
The present disclosure provides a combination disposable centrifuge tube and centrifuge device, and a PRP preparation method. The centrifugal tube comprises a first bin body with openings at two ends, a second bin body with openings at two ends and a third bin body with openings at two ends, wherein the first bin body, the second bin body and the third bin body are sequentially detachably connected through a luer bi-pass joint with a valve, and the valve can be opened or closed; wherein, the third storehouse is internal to be provided with the division board, and the division board can slide along the direction of height of the third storehouse body, and the one end that the second storehouse body was kept away from in the third storehouse body is provided with the adjusting device who is connected with the division board, can drive the division board through adjusting device and slide along the direction of height of the third storehouse body to adjust the high position of division board in the third storehouse body. The present disclosure can ensure high purity of extracted platelets without mixing red blood cells if necessary.
Description
Technical Field
Embodiments of the present disclosure relate generally to the field of medical device technology, and more particularly, to a set-up single-use centrifuge tube and centrifuge device, a PRP preparation method.
Background
The existing centrifugal tube has an integral structure, when layered blood is extracted after centrifugal treatment, a platelet layer and a red blood cell layer are positioned in the same centrifugal tube, and red blood cells are mixed in the centrifugal tube when platelets are extracted, so that the purity of the extracted platelets is insufficient, the results of subsequent tests and detection are influenced, and the clinical curative effect is further influenced.
Disclosure of Invention
In a first aspect of the disclosure, a combined disposable PRP preparation centrifuge tube is provided, which comprises a first chamber body with two open ends, a second chamber body with two open ends, and a third chamber body with two open ends, wherein the first chamber body, the second chamber body and the third chamber body are detachably connected in sequence through a luer two-way joint with a valve, and the valve can be opened or closed; the third bin body is internally provided with a separation plate, the third bin body is internally provided with a first cavity and a second cavity which are separated by the separation plate, the first cavity is positioned on one side of the separation plate close to the second bin body and can be communicated with the second bin body, the second cavity is positioned on one side of the separation plate far away from the second bin body, the separation plate can slide along the height direction of the third bin body to adjust the volume of the first cavity and the second cavity, one end of the third bin body far away from the second bin body is provided with an adjusting device connected with the separation plate, and the adjusting device can drive the separation plate to slide along the height direction of the third bin body so as to adjust the height position of the separation plate in the third bin body.
The above aspect and any possible implementation manner further provide an implementation manner, wherein the adjusting device includes a connecting rod and an adjusting cap, the adjusting cap is sleeved on an outer side wall of the third bin body and can move along the height direction of the third bin body, and the connecting rod is respectively connected with the partition plate and the adjusting cap and can drive the partition plate to slide along the height direction of the third bin body under the action of the adjusting cap.
The above-mentioned aspects and any possible implementation manner further provide an implementation manner, wherein an external thread is disposed on an outer side wall of the third cartridge body, an internal thread matched with the external thread is disposed on the adjusting cap, and the adjusting cap is screwed and mounted on the outer side wall of the third cartridge body through matching between the internal thread and the external thread.
As to the above-mentioned aspects and any possible implementation manner, an implementation manner is further provided, in which one end of the first cartridge body connected to the second cartridge body is in a closing-in structure, both ends of the second cartridge body are in a closing-in structure, and one end of the third cartridge body connected to the second cartridge body is in a closing-in structure.
The above aspect and any possible implementation manner further provide an implementation manner, wherein the closing-up structure is an inclined structure, and an inclination angle of the closing-up structure is 45-60 °.
The above-described aspect and any possible implementation manner further provide an implementation manner, wherein an injection hole assembly is disposed at the closing-in structure of the third cartridge body, and an end of the injection hole assembly, which is away from the outer surface of the third cartridge body, does not exceed a plane where the side wall of the third cartridge body is located.
The above-described aspect and any one of the possible implementations further provides an implementation in which a middle portion of an end surface of the first cartridge body distal from the end of the second cartridge body has an opening.
The above-described aspects and any possible implementation further provide an implementation, further including: the sealing plug and the ventilation plug can be arranged at the opening to plug the opening.
In a second aspect of the present disclosure, there is provided a set-up single use centrifugation device comprising: one end of the protection frame is closed, and the other end of the protection frame is open; the adjusting cover is covered at the opening of the protection frame and is in sliding connection with the opening end of the protection frame; a modular disposable centrifuge tube according to any of the first aspects, the modular disposable centrifuge tube being disposed within the protective rack.
In a third aspect of the present disclosure, there is provided a method of preparing a PRP based on the disposable assembled centrifuge tube of any one of the first aspects, comprising: injecting blood into the third bin body, and centrifuging the blood in the centrifugal tube; adjusting the height of the partition plate in the third bin body to ensure that all or most of the erythrocytes positioned at the lowermost layer are positioned in the third bin body; closing a valve of a luer two-way joint between the second bin body and the third bin body, and detaching the third bin body; centrifuging the blood in the first bin body and the second bin body again; and closing a valve of a luer two-way connector between the first bin body and the second bin body, and disassembling the first bin body to obtain the PRP in the second bin body.
In the assembled disposable centrifuge tube, the centrifuge device and the PRP preparation method provided by the embodiment of the disclosure, the centrifuge tube is composed of the first chamber body, the second chamber body and the third chamber body which are detachably connected through the luer bi-pass joint, the partition plate and the adjusting device are arranged on the third chamber body, and the position of the partition plate in the third chamber body is adjusted through the adjusting device, so that platelets are positioned in the second chamber body, thereby ensuring that the purity of the platelets is high when the platelets are extracted, and the platelets are not mixed with red blood cells when necessary.
It should be understood that the statements herein reciting aspects are not intended to limit the critical or essential features of the embodiments of the present disclosure, nor are they intended to limit the scope of the present disclosure. Other features of the present disclosure will become apparent from the following description.
Drawings
The above and other features, advantages and aspects of various embodiments of the present disclosure will become more apparent by referring to the following detailed description when taken in conjunction with the accompanying drawings. In the drawings, like or similar reference characters designate like or similar elements, and wherein:
FIG. 1 shows a schematic structural diagram of a assembled disposable centrifuge tube provided by an embodiment of the present disclosure;
FIG. 2 illustrates an assembly view of a modular disposable centrifuge tube provided by embodiments of the present disclosure;
FIG. 3 shows a schematic structural view of a modular disposable centrifuge device provided by an embodiment of the present disclosure.
Wherein,
10. a first bin body; 11. sealing the plug; 12. a breathable plug;
20. a second bin body;
30. a third bin body; 31. a partition plate; 32. a connecting rod; 33. an adjusting cap; 34. an injection orifice assembly; 341. a first blocking cap;
40. luer two-way joint; 401. a second plugging cap;
51. a protection frame; 52. and adjusting the cover.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure will be described clearly and completely with reference to the drawings in the embodiments of the present disclosure, and it is obvious that the described embodiments are some, but not all embodiments of the present disclosure. All other embodiments, which can be derived by a person skilled in the art from the embodiments disclosed herein without making any creative effort, shall fall within the protection scope of the present disclosure.
In addition, the term "and/or" herein is only one kind of association relationship describing an associated object, and means that there may be three kinds of relationships, for example, a and/or B, which may mean: a exists alone, A and B exist simultaneously, and B exists alone. In addition, the character "/" herein generally indicates that the former and latter related objects are in an "or" relationship.
Fig. 1 shows a schematic structural view of a assembled disposable centrifuge tube provided in an embodiment of the present disclosure, and fig. 2 shows an assembly view of the assembled disposable centrifuge tube provided in an embodiment of the present disclosure.
Referring to fig. 1 and 2, the centrifuge tube includes a first chamber body 10 with openings at two ends, a second chamber body 20 with openings at two ends, and a third chamber body 30 with openings at two ends, wherein the first chamber body 10, the second chamber body 20, and the third chamber body 30 are sequentially detachably connected through a luer two-way connector 40 having a valve, and the valve can be opened or closed.
A partition plate 31 is arranged in the third bin body 30, a first cavity and a second cavity which are separated by the partition plate 31 are arranged in the third bin body 30, the first cavity is positioned on one side of the partition plate 31 close to the second bin body 20 and can be communicated with the second bin body 20, the second cavity is positioned on one side of the partition plate 31 far away from the second bin body 20, the partition plate 31 can slide along the height direction of the third bin body 30, the volume size of the first cavity and the second cavity can be adjusted, an adjusting device connected with the partition plate 31 is arranged at one end, far away from the second bin body 20, of the third bin body 30, the partition plate 31 can be driven to slide along the height direction of the third bin body 30 through the adjusting device, and the height position of the partition plate 31 in the third bin body 30.
It should be noted that the two cartridge bodies are connected by the luer two-way joint 40, so that one of the cartridge bodies can be disassembled without affecting the rest of the cartridge bodies.
The separation plate 31 is connected with the inner side wall of the third cabin 30 in a sealing manner, optionally, the separation plate 31 is made of a rubber material and is connected with the inner side wall of the third cabin 30 in an interference fit manner.
In one example, from top to bottom, a first cartridge body 10, a second cartridge body 20 and a third cartridge body 30 are coaxially arranged, the first cartridge body 10 is used for storing a centrifuged blood plasma layer, the second cartridge body 20 is used for storing a centrifuged blood platelet layer, and the third cartridge body 30 is used for storing a centrifuged red blood cell layer.
When the blood centrifuge is used specifically, a valve of the luer two-way connector 40 is opened, blood is injected into the third bin body 30, the blood in the centrifuge tube is centrifuged, and the treated blood is divided into a plasma layer, a platelet layer and a red blood cell layer from top to bottom. When red blood cells need to be extracted, the height of the separation plate 31 in the third bin body 30 can be adjusted through the adjusting device, so that the volume of the first cavity is adjusted, after the height of the red blood cells reaches a proper position, a valve of the luer two-way joint 40 between the third bin body 30 and the second bin body 20 is closed, all or most of the red blood cells at the lowest layer are positioned in the first cavity according to actual needs, the third bin body 30 is detached and discarded, the first bin body 10 and the second bin body 20 are left, the luer two-way joint 40 between the third bin body 30 and the second bin body 20 is plugged by the second plugging cap 401, and then the second centrifugal treatment is carried out.
It should be noted that the second block cap 401 has a large bottom area, so that it is possible to prevent the parts from being damaged due to an excessive pressure at the lower part of the luer two-way connector 40 during centrifugal movement.
When platelets need to be extracted after the second centrifugation treatment, the valve of the luer two-way joint 40 between the first bin body 10 and the second bin body 20 is closed, the first bin body 10 is discarded, and the second bin body 20 is reserved, wherein the PRP is required.
In this embodiment, the first bin 10, the second bin 20 and the third bin 30 detachably connected by the luer two-way connector 40 together form a centrifuge tube, the third bin 30 is provided with a separation plate 31 and an adjusting device, and the position of the separation plate 31 in the third bin 30 is adjusted by the adjusting device to enable platelets to be located in the second bin 20, so that the high purity of the platelets is ensured when extracting PRP, and erythrocytes are not mixed if necessary.
In this embodiment, the first bin 10, the second bin 20 and the third bin 30 are connected in sequence to form a soft tissue type centrifuge tube or a bone tissue type centrifuge tube.
The total volume of the soft tissue type centrifuge tube can be 80ml, the corresponding blood collection amount is 60-70 ml, the volume of the first bin body 10 can be 35ml, the volume of the second bin body 20 can be 10ml, and the maximum volume of the third bin body 30 can be 45 ml. It should be noted that the third cartridge body 30 can change its capacity by adjusting the position of the separation plate 31 therein.
The total volume of the bone tissue type centrifuge tube can be 140ml, the corresponding blood collection amount is 100-120 ml, the volume of the first bin body 10 can be 60ml, the volume of the second bin body 20 can be 13ml, and the maximum volume of the third bin body 30 can be 70 ml. It should be noted that the third cartridge body 30 can change its capacity by adjusting the position of the separation plate 31 therein.
In some embodiments, the adjusting device comprises a connecting rod 32 and an adjusting cap 33, the adjusting cap 33 is sleeved on the outer side wall of the third bin body 30 and can move along the height direction of the third bin body 30, the connecting rod 32 is respectively connected with the separation plate 31 and the adjusting cap 33, and the separation plate 31 can be driven by the adjusting cap 33 to slide along the height direction of the third bin body 30. Further, an external thread is arranged on the outer side wall of the third bin body 30, an internal thread matched with the external thread is arranged on the adjusting cap 33, and the adjusting cap 33 is in threaded connection with the third bin body 30. When the position of the separation plate 31 in the third bin body 30 needs to be adjusted, the adjusting cap 33 can be screwed, so that the depth of the adjusting rod 32 extending into the third bin body 30 is changed, and further, the position of the separation plate 31 in the third bin body 30 is changed to change the volume of the third bin body 30.
In the embodiment, the adjusting cap 33 is connected with the third cartridge body 30 in a threaded manner, so that the volume of the third cartridge body 30 can be adjusted more accurately.
In some embodiments, in order to facilitate the connection between the luer bi-pass joint 40 and the three cartridges, the first cartridge 10, the second cartridge 20, and the third cartridge 30 are composed of a cylindrical portion and a closing structure in the shape of a cone, wherein the end of the first cartridge 10 connected to the second cartridge 20 is the closing structure, both ends of the second cartridge 20 are the closing structures, the end of the third cartridge 30 connected to the second cartridge 20 is the closing structure, the size of the opening at the end of the closing structure can be determined according to the size of the opening at the end of the luer bi-pass joint 40, as long as it is ensured that the first cartridge 10, the second cartridge 20, and the third cartridge 30 are respectively connected to the luer bi-pass joint 40 in a sealing manner.
Optionally, the closing-up structure is an inclined structure, that is, the closing-up structure is such that the side wall surface of the first bin body 10, the second bin body 20 or the third bin body 30 is inclined towards the axial direction thereof at the end portion thereof, and the inclination angle thereof is 45-60 °. Preferably, the angle of inclination of the cuff structure is 45 °.
In this embodiment, the closing-in structure is arranged to facilitate the connection between the two-way luer connector 40, and at the same time, the blood in the centrifuge tube can flow into the next chamber more easily.
In some embodiments, an injection hole assembly is disposed at the closing-up structure of the third cartridge body 30, and optionally, the injection hole assembly is a cylinder connected to the outer surface of the third cartridge body 30 and parallel to the bottom wall of the third cartridge body 30, the cylinder is hollow, and a channel communicated with the internal cavity of the third cartridge body 30 is formed, and blood is injected into the third cartridge body 30 through the channel. Further, one end of the injection hole assembly, which is far away from the outer surface of the third bin body 30, does not exceed the plane where the side wall of the cylindrical part of the third bin body 30 is located, so that the injection hole assembly is prevented from being broken due to collision. In this embodiment, blood can be injected into third cartridge body 30 through the injection hole assembly.
Optionally, the centrifuge tube further comprises a first cap 341, and the injection hole assembly 34 is sealed with the first cap 341 when the injection hole assembly 34 is not in use.
In some embodiments, the first cartridge body 10 has an opening in the middle of its end face distal from the second cartridge body 20. Optionally, the centrifuging tube still include with sealed end cap 11 and ventilative end cap 12 that the opening matches, sealed end cap 11 uses when the centrifugation after the intraductal blood that pours into of centrifuging into, can seal the opening, ventilative end cap 12 uses when pouring into blood into the centrifuging tube into, is equipped with fungi-proofing dustproof filter membrane in the ventilative end cap 12, when can guaranteeing that the atmospheric pressure in the centrifuging tube is the same with external atmospheric pressure, also can prevent that the bacterium in the air from entering into the centrifuging tube.
In another aspect, embodiments of the present disclosure also provide a set-up single use centrifuge device, see fig. 3, comprising a protective rack 51, an adjustment cap 52 and a centrifuge tube as provided in the embodiments described above. Wherein, one end opening is sealed to the one end of fender bracket 51, and the centrifuging tube is placed in fender bracket 51, adjusts the lid 52 lid dress at the opening part of fender bracket 51, with the open end sliding connection of fender bracket 51, adjusts the position of lid 52 through the adjustment for the bottom of fender bracket 51 and the tight fixed centrifuging tube of regulation lid 52 clamp together, further strengthened the rigidity of centrifuging tube, make the centrifuging tube not fragile when the centrifugation.
Optionally, since the centrifuge tube is composed of the first bin 10, the second bin 20 and the third bin 30, when in use, only the centrifuge tube composed of the first bin 10 and the second bin 20 may need to be centrifuged, and thus the height of the protection frame 51 is set to be less than that of the centrifuge tube.
In another aspect, embodiments of the present disclosure further provide a preparation method of a PRP, which is implemented based on the centrifugal tube in the above embodiments.
Specifically, the method comprises the following steps:
in step S1, blood is injected into the third cartridge body 30, and the blood in the centrifugal tube is centrifuged.
In this embodiment, when blood is injected into the third silo body 30, the valve of the luer two-way connector 40 between the first silo body 10 and the second silo body 20 is in an open state, the valve of the luer two-way connector 40 between the second silo body 20 and the third silo body 30 is also in an open state, a gas-permeable plug 12 is adopted at the opening of the first silo body 10, the blood is injected through the injection hole assembly 34 on the third silo body 30, after the blood is injected, the injection hole assembly 34 is sealed by the first plug cap 341, and the gas-permeable plug 12 is replaced by the sealing plug 11.
Alternatively, the centrifuge tube can be positioned laterally to inject blood into third cartridge body 30 through injection port assembly 34.
In step S2, the height of the partition plate 31 in the third cartridge body 30 is adjusted so that all or most of the red blood cells in the lowest layer are in the third cartridge body 30.
It should be noted that, in the blood after centrifugation, the red blood cells are located in the lowest layer, and the height of the partition plate 31 in the third cartridge body 30 can be adjusted to make all or most of the red blood cells located in the lowest layer be located in the third cartridge body 30. The lowermost red blood cells are predominantly disposed within the third cartridge body 30, e.g., the red blood cells occupying all of the red blood cell volume 4/5 may be disposed within the third cartridge body 30.
Step S3, the valve of the luer bi-pass fitting 40 between the second cartridge body 20 and the third cartridge body 30 is closed, and the third cartridge body 30 is removed.
In step S4, the blood in the first cartridge body 10 and the second cartridge body 20 is again centrifuged.
In the present embodiment, when the re-centrifugation treatment is carried out, the second plugging cap 401 can be used for plugging at the luer double-way joint 40 between the second cartridge body 20 and the third cartridge body 30.
Step S5, the valve of the luer bi-pass fitting 40 between the first cartridge body 10 and the second cartridge body 20 is closed, and the first cartridge body 10 is disassembled to obtain the PRP in the second cartridge body 20.
The blood in the first cartridge body 10 and the second cartridge body 20 is centrifuged for the second time, so that all PRP is located in the second cartridge body 20.
In the description of the present specification, the terms "connect", "mount", "fix", and the like are to be understood in a broad sense, for example, "connect" may be a fixed connection, a detachable connection, or an integral connection; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
In the description of the present application, the description of the terms "one embodiment," "some embodiments," etc. means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (10)
1. The assembled disposable centrifuge tube is used for preparing PRP and is characterized by comprising a first chamber body with openings at two ends, a second chamber body with openings at two ends and a third chamber body with openings at two ends, wherein the first chamber body, the second chamber body and the third chamber body are sequentially detachably connected through a luer bi-pass joint with a valve, and the valve can be opened or closed;
the third bin body is internally provided with a separation plate, the third bin body is internally provided with a first cavity and a second cavity which are separated by the separation plate, the first cavity is positioned on one side of the separation plate close to the second bin body and can be communicated with the second bin body, the second cavity is positioned on one side of the separation plate far away from the second bin body, the separation plate can slide along the height direction of the third bin body to adjust the volume of the first cavity and the second cavity, one end of the third bin body far away from the second bin body is provided with an adjusting device connected with the separation plate, and the adjusting device can drive the separation plate to slide along the height direction of the third bin body so as to adjust the height position of the separation plate in the third bin body.
2. The assembled disposable centrifuge tube of claim 1, wherein the adjusting device comprises a connecting rod and an adjusting cap, the adjusting cap is sleeved on the outer side wall of the third chamber body and can move along the height direction of the third chamber body, the connecting rod is respectively connected with the separating plate and the adjusting cap, and the separating plate can be driven to slide along the height direction of the third chamber body under the action of the adjusting cap.
3. The assembled disposable centrifuge tube of claim 2, wherein the outer sidewall of the third chamber body is provided with an external thread, the adjusting cap is provided with an internal thread matching with the external thread, and the adjusting cap is screwed on the outer sidewall of the third chamber body by matching the internal thread with the external thread.
4. The assembled disposable centrifuge tube of claim 1, wherein the first and second chambers are connected to each other by a closing structure, the second chamber is connected to each other by a closing structure, and the third chamber is connected to the second chamber by a closing structure.
5. The assembled disposable centrifuge tube of claim 4, wherein the closing-up structure is an inclined structure with an angle of 45-60 °.
6. The assembled disposable centrifuge tube of claim 1, wherein the closing structure of the third chamber body is provided with an injection hole assembly, and one end of the injection hole assembly, which is far away from the outer surface of the third chamber body, does not exceed the plane of the side wall of the third chamber body.
7. The assembled disposable centrifuge tube of claim 1, wherein the first chamber body has an opening in the middle of the end face of the end thereof distal from the second chamber body.
8. The assembled disposable centrifuge tube of claim 7, further comprising:
the sealing plug and the ventilation plug can be arranged at the opening to plug the opening.
9. A modular single use centrifugation device for the preparation of PRP, comprising:
one end of the protection frame is closed, and the other end of the protection frame is open;
the adjusting cover is covered at the opening of the protection frame and is in sliding connection with the opening end of the protection frame;
a assembled disposable centrifuge tube according to any of claims 1 to 8 disposed within the protective rack.
10. A method of preparing a PRP based on the assembled disposable centrifuge tube of any of claims 1 to 8, comprising:
injecting blood into the third bin body, and centrifuging the blood in the centrifugal tube;
adjusting the height of the partition plate in the third bin body to ensure that all or most of the erythrocytes positioned at the lowermost layer are positioned in the third bin body;
closing a valve of a luer two-way joint between the second bin body and the third bin body, and detaching the third bin body;
centrifuging the blood in the first bin body and the second bin body again;
and closing a valve of a luer two-way connector between the first bin body and the second bin body, and disassembling the first bin body to obtain the PRP in the second bin body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911250424.2A CN111036421B (en) | 2019-12-09 | 2019-12-09 | Combined type disposable centrifuge tube, centrifugal device and PRP preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911250424.2A CN111036421B (en) | 2019-12-09 | 2019-12-09 | Combined type disposable centrifuge tube, centrifugal device and PRP preparation method |
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| CN111036421A true CN111036421A (en) | 2020-04-21 |
| CN111036421B CN111036421B (en) | 2024-05-17 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112371198A (en) * | 2020-11-24 | 2021-02-19 | 康膝生物医疗(深圳)有限公司 | Device and method for preparing injection type autologous i-PRF |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102802750A (en) * | 2009-06-16 | 2012-11-28 | 巴奥米特生物技术有限责任公司 | Liquid Separation From Adipose Tissue |
| CN103038333A (en) * | 2010-05-20 | 2013-04-10 | 里波热姆斯国际公司 | Device and method for preparing tissue, particularly adipose tissue |
| CN103842001A (en) * | 2011-12-29 | 2014-06-04 | 阿梅迪卡株式会社 | Integrated kit for separating blood and concentrating prp and method for extracting prp using same |
| CN106582908A (en) * | 2016-11-30 | 2017-04-26 | 深圳市达科为生物工程有限公司 | Platelet-rich plasma rapid separating device and method |
| CN107377238A (en) * | 2017-08-15 | 2017-11-24 | 维堪医美（厦门）科技有限公司 | A kind of platelet rich plasma separating extraction device |
| CN206793925U (en) * | 2017-04-20 | 2017-12-26 | 武汉朗克医疗器械有限公司 | Platelet rich plasma centrifugal device |
| CN207357368U (en) * | 2017-08-15 | 2018-05-15 | 维堪医美（厦门）科技有限公司 | A kind of platelet rich plasma separating extraction device |
| CN207533415U (en) * | 2017-11-08 | 2018-06-26 | 汪冬青 | Platelet rich plasma processing unit |
| CN109982731A (en) * | 2016-10-03 | 2019-07-05 | 泰尔茂比司特公司 | Centrifugal fluid separation equipment |
| CN110026297A (en) * | 2018-01-12 | 2019-07-19 | 瑞崴制造股份有限公司 | Body fluid separator |
-
2019
- 2019-12-09 CN CN201911250424.2A patent/CN111036421B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102802750A (en) * | 2009-06-16 | 2012-11-28 | 巴奥米特生物技术有限责任公司 | Liquid Separation From Adipose Tissue |
| CN103038333A (en) * | 2010-05-20 | 2013-04-10 | 里波热姆斯国际公司 | Device and method for preparing tissue, particularly adipose tissue |
| CN103842001A (en) * | 2011-12-29 | 2014-06-04 | 阿梅迪卡株式会社 | Integrated kit for separating blood and concentrating prp and method for extracting prp using same |
| CN109982731A (en) * | 2016-10-03 | 2019-07-05 | 泰尔茂比司特公司 | Centrifugal fluid separation equipment |
| CN106582908A (en) * | 2016-11-30 | 2017-04-26 | 深圳市达科为生物工程有限公司 | Platelet-rich plasma rapid separating device and method |
| CN206793925U (en) * | 2017-04-20 | 2017-12-26 | 武汉朗克医疗器械有限公司 | Platelet rich plasma centrifugal device |
| CN107377238A (en) * | 2017-08-15 | 2017-11-24 | 维堪医美（厦门）科技有限公司 | A kind of platelet rich plasma separating extraction device |
| CN207357368U (en) * | 2017-08-15 | 2018-05-15 | 维堪医美（厦门）科技有限公司 | A kind of platelet rich plasma separating extraction device |
| CN207533415U (en) * | 2017-11-08 | 2018-06-26 | 汪冬青 | Platelet rich plasma processing unit |
| CN110026297A (en) * | 2018-01-12 | 2019-07-19 | 瑞崴制造股份有限公司 | Body fluid separator |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112371198A (en) * | 2020-11-24 | 2021-02-19 | 康膝生物医疗(深圳)有限公司 | Device and method for preparing injection type autologous i-PRF |
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| CN111036421B (en) | 2024-05-17 |
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