CN112673955A - Rapid screening method for quinoa nitrogen efficient genotype breeding resources - Google Patents
Rapid screening method for quinoa nitrogen efficient genotype breeding resources Download PDFInfo
- Publication number
- CN112673955A CN112673955A CN202011590180.5A CN202011590180A CN112673955A CN 112673955 A CN112673955 A CN 112673955A CN 202011590180 A CN202011590180 A CN 202011590180A CN 112673955 A CN112673955 A CN 112673955A
- Authority
- CN
- China
- Prior art keywords
- quinoa
- nitrogen
- root
- leaf
- leaves
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of agriculture, and discloses a rapid screening method of a quinoa nitrogen efficient genotype breeding resource, which comprises the following steps: selecting single plants with good adaptability, consistent seed properties and good disease resistance, selecting excellent materials through indoor seed test, threshing and storing; screening, pretreating and water culturing the preserved quinoa seeds; when the quinoa reaches the bud stage, performing morphological evaluation on the quinoa; and sampling treatment is carried out; by means of H2SO4‑H2O2Digesting the cultured quinoa, and determining the total nitrogen content of the quinoa by using a full-automatic azotometer; and calculating the accumulation amount and the physiological utilization efficiency of nitrogen in the chenopodium quinoa, and screening the chenopodium quinoa nitrogen efficient genotype breeding resources based on the calculation result. The invention effectively screens out the variety resource of the nitrogen high-efficiency genotype from the form, the nitrogen accumulation amount and the physiological utilization efficiency, and finally the bred breeding material can be directly used for the quinoa nitrogen high-efficiency geneHas good market application prospect in breeding new species.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a rapid screening method of a quinoa nitrogen efficient genotype breeding resource.
Background
At present, excessive application of nitrogen fertilizer becomes one of the main reasons that the problems of low utilization efficiency of nitrogen fertilizer, large accumulation of soil nitrogen, non-point source pollution and the like are increasingly serious in China, screening of crop nitrogen high-efficiency genotypes from the genetic potential of crops becomes an important way for reducing the application amount of the nitrogen fertilizer and improving the nutritional efficiency of the crop nitrogen, and meanwhile, the research on the physiological mechanism of the nitrogen high-efficiency genotypes can provide an effective way and a theoretical basis for variety genetic improvement. However, the evaluation indexes and evaluation methods of the nitrogen-efficient genotypes of the crops still do not form a unified standard so far, and the physiological mechanisms of the nitrogen-efficient genotypes are not deeply researched.
Through the above analysis, the problems and defects of the prior art are as follows: the prior art does not have a related research of rapid screening of quinoa nitrogen high-efficiency genotype breeding resources.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a rapid screening method of a quinoa nitrogen efficient genotype breeding resource.
The invention is realized in such a way that the rapid screening method of the quinoa nitrogen efficient genotype breeding resource comprises the following steps:
selecting single plants with good adaptability, consistent seed properties and good disease resistance, selecting excellent chenopodium quinoa materials through indoor seed test, threshing, storing and standby.
Screening, pretreating and disinfecting stored quinoa seeds; and soaking the disinfected seeds in a nutrient solution containing trace elements for 4-7 hours, and transferring the seeds into a germination accelerating room for accelerating germination to obtain the germinated quinoa seeds.
Thirdly, carrying out matrix seedling raising on the germinated quinoa seeds, and treating the germinated quinoa seeds by using growth hormone; soaking quinoa treated by growth hormone into nutrient solution for root inducing culture, and finishing the root inducing culture when the seedling age is 8 leaves and the length of the root system is more than or equal to 5cm to obtain the quinoa seedling after water culture.
Step four, when the quinoa seedlings are unfolded into 2 leaves, adding complete nutrient solution for seedling; and when the chenopodium quinoa reaches the bud stage, randomly selecting n chenopodium quinoa plant roots, growing points and leaves for scanning.
Analyzing and measuring the average root diameter, the total root length, the root surface area and the root volume, and the grading ranges of different root diameters, the total root length, the root surface area and the root volume, counting the root system injury index, and generating a root system development index; the calculation formula of the root injury index is as follows:
the root injury index% { Σ (number of plants × number of stages) }/(total number of plants × highest number of stages) × 100.
Step six, detecting the growth point state of the quinoa plant, and judging the growth point state; simultaneously, randomly extracting 10 quinoa plants from each material, taking 1 to 5 leaves from a growing point of each plant downwards to judge the yellowing degree of the leaves, and calculating the yellowing index of the leaves; the calculation formula of the leaf yellowing index is as follows:
the leaf yellowing index% { Σ (number of yellowing stage leaves × number of yellowing stage stages) }/(total number of leaves × number of yellowing heaviest stage stages) × 100.
And seventhly, judging the nitrogen efficient genotype and the nitrogen inefficient genotype of the chenopodium quinoa plant based on the root development index, the growth point state and the leaf yellowing degree of the chenopodium quinoa plant to generate a morphological evaluation result.
Step eight, sampling treatment: washing collected quinoa samples with tap water and deionized water in sequence, wiping the washed samples with absorbent paper, deactivating enzyme at 108 ℃ for 25-30 min, cooling to 70 ℃, drying for 20-24 h until the water content is 5% -10%, crushing by using a stainless steel crusher, and sieving.
And step nine, adding ethyl acetate into the quinoa powder, extracting for 30-45 min by using an ultrasonic cleaner, filtering and collecting, repeatedly performing ultrasonic extraction on filter residues for 2-4 times, combining the obtained filtrate, concentrating and fixing the volume for later use.
And step ten, adding a sodium nitrite-sodium molybdate reagent and a sodium hydroxide solution into the quinoa extract obtained in the step nine for color development, carrying out image scaling, image graying, image binarization and image edge detection on the developed image through a color development recognizer, and recognizing the developed color.
Step eleven, utilizing H2SO4-H2O2And (4) carrying out digestion treatment on the quinoa subjected to crushing and sieving treatment in the step eight, and determining the total nitrogen content of the quinoa by using a full-automatic azotometer.
And step twelve, calculating the accumulation amount and the physiological utilization efficiency of nitrogen in the chenopodium quinoa, and screening the chenopodium quinoa nitrogen efficient genotype breeding resources based on the calculation result.
Further, in the second step, the method for sterilizing the seeds comprises: subjecting the seeds to 30% hydrogen peroxide and 0.1% NaC1O3And (5) performing disinfection treatment.
Further, in the second step, the temperature of the germination accelerating treatment is 25-28 ℃.
Further, in the third step, the formula of the substrate for raising the seedlings by the substrate is grass peat: perlite: and adding 0.4-0.5 kg of NPK compound fertilizer with the element mass ratio of 17:17:17 into each cubic meter of vermiculite as 6:3: 1.
Further, in the third step, the growth hormone is composed of, by mass, 6-10 parts of naphthoic acid, 2-5 parts of gibberellin, 5-8 parts of indoleacetic acid and 3-6 parts of salicylic acid.
Further, in the third step, the quinoa treated by the growth hormone is immersed in the nutrient solution for root induction culture at the culture temperature of 20-25 ℃, the dissolved oxygen content is 3.0-5.5 mg/L, the EC value is 0.5-1.3 ms/cm, and the pH value is 5.7-6.5.
Further, in the fourth step, the selecting n chenopodium quinoa plant roots comprises: n is not less than 8.
Further, in the fifth step, the root development index is classified into 5 grades, including:
level 1: more new roots, thick and long roots, non-withered root tips and white color;
and 2, stage: more new roots, thick and long roots, slightly withered root tips and white color;
and 3, level: the new roots are few, the roots are thick and short, the root tips are withered and brown;
4, level: the new roots are few, the roots are thick and short, the root tips are withered, and the color is brown;
and 5, stage: no root growth, severe withered root tip, and brown color.
Further, in the sixth step, the index of yellowing of the leaves is classified into 5 grades, including:
level 0: normal green leaves;
level 1: the color of the leaf is slightly yellow-green, the base part of the leaf is yellow, and most of the leaf tip is still green;
and 2, stage: yellow green, the whole leaf is more yellow green than the normal leaf;
and 3, level: yellow, the whole leaf appears yellow compared to normal leaf; or the color of the leaves fades slightly but the leaves appear small withered spots;
4, level: white-yellow, the whole leaf appears white-yellow than normal leaf; or the superior leaflet along the veins with large transparent necrotic spots.
Further, in the twelfth step, the method for calculating the nitrogen accumulation amount and the physiological utilization efficiency includes:
the nitrogen accumulation amount is the nitrogen absorption amount of the chenopodium quinoa single plant, and the physiological utilization efficiency of nitrogen is the dry matter amount formed by the nitrogen absorption amount of the unit of the nitrogen accumulation amount.
By combining all the technical schemes, the invention has the advantages and positive effects that: the method effectively screens out variety resources of nitrogen efficient genotypes from morphology, nitrogen accumulation and physiological utilization efficiency, determines three indexes of growing point state, leaf yellowing index and root development index as evaluation indexes, and establishes a screening method of chenopodium quinoa efficient genotype breeding resources; meanwhile, the screening method constructed by the invention is based on scientific experiments, and finally the screened breeding material can be directly used for breeding new varieties of quinoa nitrogen-efficient genotype, so that the screening method has good market application prospect.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of a rapid screening method of a quinoa nitrogen efficient genotype breeding resource provided by an embodiment of the invention.
Fig. 2 is a flowchart of a method for performing seedling raising treatment on quinoa seeds according to an embodiment of the present invention.
Fig. 3 is a flowchart of a method for evaluating morphology of quinoa according to an embodiment of the present invention.
Fig. 4 is a flowchart of a method for sampling quinoa according to an embodiment of the present invention.
FIG. 5 is a flowchart of a method for developing and identifying a quinoa extract according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a rapid screening method of a quinoa nitrogen efficient genotype breeding resource, and the invention is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the rapid screening method for a quinoa nitrogen-efficient genotype breeding resource provided by the embodiment of the present invention includes:
s101, selecting single plants with good adaptability, consistent seed properties and good disease resistance, selecting excellent materials through indoor seed test, threshing, storing and reserving for later use.
S102, screening, pretreating and seedling raising the stored quinoa seeds.
S103, when the chenopodium quinoa reaches the bud stage, randomly selecting n chenopodium quinoa plant roots, growing points and leaves for scanning, performing shape evaluation on the chenopodium quinoa, and performing sampling treatment.
S104, obtaining a quinoa extract, adding a sodium nitrite-sodium molybdate reagent and a sodium hydroxide solution into the quinoa extract for developing, and identifying the color development.
S105, using H2SO4-H2O2Digesting the cultured quinoa, and determining the total nitrogen content of the quinoa by using a full-automatic azotometer.
S106, calculating the accumulation amount and the physiological utilization efficiency of nitrogen in the quinoa, and screening the quinoa nitrogen efficient genotype breeding resources based on the calculation result.
As shown in fig. 2, in step S102 provided in the embodiment of the present invention, the method for performing seedling raising treatment on quinoa seeds includes:
s201, screening, pretreating and disinfecting the stored quinoa seeds.
S202, soaking the disinfected seeds in a nutrient solution containing trace elements for 4-7 hours, and transferring the seeds into a germination accelerating room for accelerating germination to obtain the germinated quinoa seeds.
S203, firstly, carrying out substrate seedling raising on the germinated quinoa seeds, and treating the germinated quinoa seeds by utilizing growth hormone.
And S204, soaking the quinoa treated by the growth hormone into a nutrient solution for root induction culture, and finishing the root induction culture when the quinoa is 8 leaves in seedling age and the length of a root system is more than or equal to 5cm to obtain the quinoa seedlings subjected to water culture.
S205, when the quinoa seedlings are unfolded to 2 leaves, the quinoa seedlings are transplanted into a complete nutrient solution for seedling.
In step S201 provided in the embodiment of the present invention, the method for disinfecting seeds includes: subjecting the seeds to 30% hydrogen peroxide and 0.1% NaC1O3And (5) performing disinfection treatment.
In step S202 provided in the embodiment of the present invention, the temperature of the germination accelerating treatment is 25 to 28 ℃.
In step S202 provided in the embodiment of the present invention, the substrate formula for substrate seedling culture is peat: perlite: and adding 0.4-0.5 kg of NPK compound fertilizer with the element mass ratio of 17:17:17 into each cubic meter of vermiculite as 6:3: 1.
In step S203 provided by the embodiment of the present invention, the growth hormone is composed of, by mass, 6 to 10 parts of naphthoic acid, 2 to 5 parts of gibberellin, 5 to 8 parts of indoleacetic acid, and 3 to 6 parts of salicylic acid.
In step S204 provided by the embodiment of the present invention, the cultivation temperature for immersing quinoa treated with growth hormone into the nutrient solution for root induction cultivation is 20 ℃ to 25 ℃, the dissolved oxygen content is 3.0mg/L to 5.5mg/L, the EC value is 0.5ms/cm to 1.3ms/cm, and the pH value is 5.7 to 6.5.
In step S103 provided by the embodiment of the present invention, the selecting root systems of n chenopodium quinoa plants includes: n is not less than 8.
As shown in fig. 3, in step S103, the method for evaluating the morphology of quinoa according to the embodiment of the present invention includes:
s301, analyzing and measuring the average root diameter, the total root length, the root surface area and the root volume, and the grading ranges of different root diameters, the total root length, the root surface area and the root volume, counting the root system injury index, and generating a root system development index.
S302, detecting the growth point state of the quinoa plant, and judging the growth point state; meanwhile, 10 quinoa plants are randomly extracted from each material, 1 to 5 leaves are downwards taken from a growing point of each plant to judge the yellowing degree of the leaves, and the yellowing index of the leaves is calculated.
S303, judging the nitrogen efficient genotype and the nitrogen inefficient genotype of the chenopodium quinoa plant based on the root development index, the growth point state and the leaf yellowing degree of the chenopodium quinoa plant to generate a morphological evaluation result.
In step S301 provided in the embodiment of the present invention, the calculation formula of the root injury index is:
the root injury index% { Σ (number of plants × number of stages) }/(total number of plants × highest number of stages) × 100.
In step S302 provided in the embodiment of the present invention, a calculation formula of the leaf yellowing index is:
the leaf yellowing index% { Σ (number of yellowing stage leaves × number of yellowing stage stages) }/(total number of leaves × number of yellowing heaviest stage stages) × 100.
In step S301 provided in the embodiment of the present invention, the root development index is classified into 5 grades, and includes:
level 1: more new roots, thick and long roots, non-withered root tips and white color;
and 2, stage: more new roots, thick and long roots, slightly withered root tips and white color;
and 3, level: the new roots are few, the roots are thick and short, the root tips are withered and brown;
4, level: the new roots are few, the roots are thick and short, the root tips are withered, and the color is brown;
and 5, stage: no root growth, severe withered root tip, and brown color.
In step S302 provided in the embodiment of the present invention, the blade yellowing index is classified into 5 stages, and includes:
level 0: normal green leaves;
level 1: the color of the leaf is slightly yellow-green, the base part of the leaf is yellow, and most of the leaf tip is still green;
and 2, stage: yellow green, the whole leaf is more yellow green than the normal leaf;
and 3, level: yellow, the whole leaf appears yellow compared to normal leaf; or the color of the leaves fades slightly but the leaves appear small withered spots;
4, level: white-yellow, the whole leaf appears white-yellow than normal leaf; or the superior leaflet along the veins with large transparent necrotic spots.
As shown in fig. 4, in step S103 provided in the embodiment of the present invention, the method for performing sampling processing on quinoa includes:
s401, washing collected quinoa samples with tap water and deionized water in sequence, wiping the washed samples with absorbent paper, and deactivating enzyme at 108 ℃ for 25-30 min.
S402, cooling to 70 ℃, drying for 20-24 hours until the water content is 5% -10%, crushing by a stainless steel crusher, and sieving.
As shown in fig. 5, in step S104, the method for developing and identifying a quinoa extract includes:
s501, adding ethyl acetate into quinoa wheat flour, extracting for 30-45 min by using an ultrasonic cleaner, filtering and collecting, repeatedly performing ultrasonic extraction on filter residues for 2-4 times, combining the obtained filtrate, concentrating and fixing the volume for later use.
S502, adding a sodium nitrite-sodium molybdate reagent and a sodium hydroxide solution into the quinoa extract for color development.
In S503, the color identifier performs scaling of the image, graying of the image, binarization of the image, and edge detection of the image on the color-developed image, thereby identifying the color development.
In step S105 provided in the embodiment of the present invention, the method for calculating the nitrogen accumulation amount and the physiological utilization efficiency includes: the nitrogen accumulation amount is the nitrogen absorption amount of the chenopodium quinoa single plant, and the physiological utilization efficiency of nitrogen is the dry matter amount formed by the nitrogen absorption amount of the unit of the nitrogen accumulation amount.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A rapid screening method of a quinoa nitrogen efficient genotype breeding resource is characterized by comprising the following steps:
selecting single plants with good adaptability, consistent seed properties and good disease resistance, selecting excellent chenopodium quinoa materials through indoor seed test, threshing, storing for later use;
screening, pretreating and disinfecting stored quinoa seeds; soaking the disinfected seeds in a nutrient solution containing trace elements for 4-7 h, and transferring the seeds into a germination accelerating room for accelerating germination to obtain germinated quinoa seeds;
thirdly, carrying out matrix seedling raising on the germinated quinoa seeds, and treating the germinated quinoa seeds by using growth hormone; soaking quinoa treated by growth hormone into a nutrient solution for root induction culture, and finishing the root induction culture when the quinoa is 8 leaves in seedling age and the length of a root system is more than or equal to 5cm to obtain the quinoa seedlings subjected to water culture;
step four, when the quinoa seedlings are unfolded into 2 leaves, adding complete nutrient solution for seedling; when the chenopodium quinoa reaches the bud stage, randomly selecting n chenopodium quinoa plant roots, growing points and leaves for scanning;
analyzing and measuring the average root diameter, the total root length, the root surface area and the root volume, and the grading ranges of different root diameters, the total root length, the root surface area and the root volume, counting the root system injury index, and generating a root system development index; the calculation formula of the root injury index is as follows:
root injury index { ∑ (number of plants × number of stages) }/(total number of plants × highest number of stages) × 100;
step six, detecting the growth point state of the quinoa plant, and judging the growth point state; simultaneously, randomly extracting 10 quinoa plants from each material, taking 1 to 5 leaves from a growing point of each plant downwards to judge the yellowing degree of the leaves, and calculating the yellowing index of the leaves; the calculation formula of the leaf yellowing index is as follows:
the leaf yellowing index% { ∑ (yellowing stage leaf number × yellowing stage number) }/(total leaf number × yellowing heaviest stage number) × 100;
seventhly, judging the nitrogen efficient genotype and the nitrogen inefficient genotype of the chenopodium quinoa plant based on the root development index, the growth point state and the leaf yellowing degree of the chenopodium quinoa plant to generate a morphological evaluation result;
step eight, sampling treatment: washing collected quinoa samples with tap water and deionized water in sequence, wiping the washed samples with absorbent paper, deactivating enzyme at 108 ℃ for 25-30 min, cooling to 70 ℃, drying for 20-24 h until the water content is 5% -10%, crushing by using a stainless steel crusher, and sieving;
step nine, adding ethyl acetate into quinoa powder, extracting for 30-45 min by using an ultrasonic cleaner, filtering and collecting, repeatedly performing ultrasonic extraction on filter residues for 2-4 times, combining the obtained filtrate, concentrating and fixing the volume for later use;
adding a sodium nitrite-sodium molybdate reagent and a sodium hydroxide solution into the quinoa extract obtained in the ninth step for developing, carrying out image scaling, image graying, image binarization and image edge detection on the developed image through a color development recognizer, and recognizing the developed color;
step eleven, utilizing H2SO4-H2O2Carrying out digestion treatment on the quinoa subjected to crushing and screening treatment in the step eight, and measuring the total nitrogen content of the quinoa by using a full-automatic azotometer;
and step twelve, calculating the accumulation amount and the physiological utilization efficiency of nitrogen in the chenopodium quinoa, and screening the chenopodium quinoa nitrogen efficient genotype breeding resources based on the calculation result.
2. The rapid screening method of quinoa nitrogen-efficient genotype breeding resources as claimed in claim 1, wherein in the second step, the method for sterilizing the seeds comprises: subjecting the seeds to 30% hydrogen peroxide and 0.1% NaC1O3And (5) performing disinfection treatment.
3. The rapid screening method of quinoa nitrogen-efficient genotype breeding resources according to claim 1, wherein in the second step, the temperature of the germination accelerating treatment is 25-28 ℃.
4. The rapid screening method of quinoa nitrogen highly efficient genotype breeding resources as claimed in claim 1, wherein in step three, the substrate formula of the substrate seedling is peat: perlite: and adding 0.4-0.5 kg of NPK compound fertilizer with the element mass ratio of 17:17:17 into each cubic meter of vermiculite as 6:3: 1.
5. The rapid screening method of quinoa nitrogen-efficient genotype breeding resources as claimed in claim 1, wherein in step three, the growth hormone is composed of, by mass, 6-10 parts of naphthoic acid, 2-5 parts of gibberellin, 5-8 parts of indoleacetic acid and 3-6 parts of salicylic acid.
6. The method for rapidly screening a quinoa nitrogen-efficient genotype breeding resource according to claim 1, wherein in step three, the cultivation temperature for immersing quinoa after being treated by growth hormone in a nutrient solution for root induction cultivation is 20-25 ℃, the dissolved oxygen content is 3.0-5.5 mg/L, the EC value is 0.5-1.3 ms/cm, and the pH value is 5.7-6.5.
7. The rapid screening method of a quinoa nitrogen-efficient genotype breeding resource of claim 1, wherein in the fourth step, the selection of n root systems of quinoa plants comprises: n is not less than 8.
8. The rapid screening method of quinoa nitrogen-efficient genotype breeding resources as claimed in claim 1, wherein in step five, the root development index is classified into 5 grades, comprising:
level 1: more new roots, thick and long roots, non-withered root tips and white color;
and 2, stage: more new roots, thick and long roots, slightly withered root tips and white color;
and 3, level: the new roots are few, the roots are thick and short, the root tips are withered and brown;
4, level: the new roots are few, the roots are thick and short, the root tips are withered, and the color is brown;
and 5, stage: no root growth, severe withered root tip, and brown color.
9. The rapid screening method of quinoa nitrogen-efficient genotype breeding resources as claimed in claim 1, wherein in step six, the leaf yellowing index is classified into 5 grades, comprising:
level 0: normal green leaves;
level 1: the color of the leaf is slightly yellow-green, the base part of the leaf is yellow, and most of the leaf tip is still green;
and 2, stage: yellow green, the whole leaf is more yellow green than the normal leaf;
and 3, level: yellow, the whole leaf appears yellow compared to normal leaf; or the color of the leaves fades slightly but the leaves appear small withered spots;
4, level: white-yellow, the whole leaf appears white-yellow than normal leaf; or the superior leaflet along the veins with large transparent necrotic spots.
10. The rapid screening method of quinoa nitrogen highly efficient genotype breeding resources as claimed in claim 1, wherein in the twelfth step, the calculation method of the nitrogen accumulation amount and the physiological utilization efficiency is as follows:
the nitrogen accumulation amount is the nitrogen absorption amount of the chenopodium quinoa single plant, and the physiological utilization efficiency of nitrogen is the dry matter amount formed by the nitrogen absorption amount of the unit of the nitrogen accumulation amount.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011590180.5A CN112673955A (en) | 2020-12-28 | 2020-12-28 | Rapid screening method for quinoa nitrogen efficient genotype breeding resources |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011590180.5A CN112673955A (en) | 2020-12-28 | 2020-12-28 | Rapid screening method for quinoa nitrogen efficient genotype breeding resources |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112673955A true CN112673955A (en) | 2021-04-20 |
Family
ID=75454862
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011590180.5A Pending CN112673955A (en) | 2020-12-28 | 2020-12-28 | Rapid screening method for quinoa nitrogen efficient genotype breeding resources |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112673955A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103058485A (en) * | 2012-12-24 | 2013-04-24 | 华南农业大学 | Method of achieving synchronization of municipal sludge desiccation and nutrient utilization |
| CN104396728A (en) * | 2014-11-06 | 2015-03-11 | 青岛农业大学 | Dry pepper Fe-efficient breeding resource rapid screening method |
| CN106416716A (en) * | 2016-10-11 | 2017-02-22 | 甘肃省农业科学院畜草与绿色农业研究所 | Breeding method of new quinoa variety |
| CN106613846A (en) * | 2016-12-16 | 2017-05-10 | 福建百卉花艺有限公司 | Water culture method |
| CN107306782A (en) * | 2017-08-16 | 2017-11-03 | 闽南师范大学 | A kind of water spinach ciltivating process rich in trace element |
| CN109964761A (en) * | 2019-04-26 | 2019-07-05 | 山东省农业科学院生物技术研究中心 | A kind of salt-soda soil quinoa ecological efficient growing and cultivation method |
| CN110915649A (en) * | 2019-12-03 | 2020-03-27 | 西藏自治区农牧科学院蔬菜研究所 | Rapid screening method of high-pungency pepper breeding resources |
-
2020
- 2020-12-28 CN CN202011590180.5A patent/CN112673955A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103058485A (en) * | 2012-12-24 | 2013-04-24 | 华南农业大学 | Method of achieving synchronization of municipal sludge desiccation and nutrient utilization |
| CN104396728A (en) * | 2014-11-06 | 2015-03-11 | 青岛农业大学 | Dry pepper Fe-efficient breeding resource rapid screening method |
| CN106416716A (en) * | 2016-10-11 | 2017-02-22 | 甘肃省农业科学院畜草与绿色农业研究所 | Breeding method of new quinoa variety |
| CN106613846A (en) * | 2016-12-16 | 2017-05-10 | 福建百卉花艺有限公司 | Water culture method |
| CN107306782A (en) * | 2017-08-16 | 2017-11-03 | 闽南师范大学 | A kind of water spinach ciltivating process rich in trace element |
| CN109964761A (en) * | 2019-04-26 | 2019-07-05 | 山东省农业科学院生物技术研究中心 | A kind of salt-soda soil quinoa ecological efficient growing and cultivation method |
| CN110915649A (en) * | 2019-12-03 | 2020-03-27 | 西藏自治区农牧科学院蔬菜研究所 | Rapid screening method of high-pungency pepper breeding resources |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bavaresco et al. | Effect of calcareous soil on photosynthesis rate, mineral nutrition, and Source‐Sink ratio of table grape | |
| CN106834418A (en) | The method that seedling stage water planting is inoculated with Rapid identification tomato neckrot Resistance To Root Rot Disease plant | |
| CN108337951A (en) | A method of reducing sloping upland water and soil and soil nitrogen loss amount | |
| CN111386790A (en) | Method for identifying low-temperature germination capacity of direct-seeded rice in cold region | |
| CN110583261B (en) | Method for planting Sasa sagittifolia by using biological fertilizer | |
| US11254907B2 (en) | Fungal volatile organic compound enhances plant s growth characteristics | |
| CN111296025A (en) | Research and application of controlled-release nitrogen fertilizer and controlled-release potassium fertilizer on influence on physiological characteristics and soil nutrients of corn in large-horn-mouth period | |
| Dudits et al. | Triploid hybrid vigor in above-ground growth and methane fermentation efficiency of energy willow | |
| CN103205501B (en) | A kind of method identifying rice blast-resistant gene of wild rice | |
| CN112673955A (en) | Rapid screening method for quinoa nitrogen efficient genotype breeding resources | |
| CN110106194A (en) | The ORF segment of POD P7 gene and its application in raising plant cadmium stress tolerance, reduction Cd accumulation | |
| CN105316240A (en) | Rhizopycnis sp. NYN8G01 and application thereof | |
| CN113099774A (en) | Screening method of low-cadmium high-quality rice | |
| CN109315286B (en) | Method for screening corn genotype for improving nitrogen efficiency of corn/soybean intercropping system | |
| CN110612837A (en) | Method for rapidly identifying leaf vegetable cadmium low-absorption variety by utilizing cadmium-rich matrix cultivation | |
| Ballhorn et al. | Quantitative effects of soil salinity on the symbiosis of wild lima bean (Phaseolus lunatus L.) and Bradyrhizobium in Costa Rica | |
| CN112243856A (en) | Early-maturing late-japonica rice hybrid breeding method | |
| CN109673201A (en) | A kind of method that shiny-leaved yellowhorn seed dormancy is abolished | |
| Weijia et al. | Use of Ginkgo biloba leaf compost for promoting soil properties and rooting of New Guinea impatiens cuttings | |
| CN113105293B (en) | Exogenous salicylic acid-added rice seedling culture solution and application thereof | |
| Hamakawa et al. | Observation of microbial colonization on the surface of rice roots along with their development and degradation | |
| US20220127561A1 (en) | Fungal volatile organic compound enhances plant growth characteristics | |
| Jafarian et al. | Histological responses of two wheat species to Azospirillum inoculation under dryland farming | |
| CN106688546A (en) | Method for improving sensitivity and specificity of soil cadmium contamination by bryophytes | |
| Al AsadA et al. | The Diversity of Arbuscular Mycorrhizal Fungi in the Black Cumin Rhizosphere (Nigella sativa L.) in Cianjur, West Java, Indonesia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210420 |