CN112662593A - Organic fertilizer fermentation inoculant and culture method thereof - Google Patents
Organic fertilizer fermentation inoculant and culture method thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to the technical field of organic fertilizer fermentation, in particular to an organic fertilizer fermentation microbial inoculum and a culture method thereof, wherein the fermentation microbial inoculum can accelerate the fermentation and decomposition speed of various organic waste gas substances, reduce the pile-turning times, shorten the fermentation and decomposition time and improve the practicability, the fermentation microbial inoculum comprises a main fermentation microbial inoculum and an auxiliary fermentation microbial inoculum, the use ratio of the main fermentation microbial inoculum to the auxiliary fermentation microbial inoculum is 4-6: 1, wherein the main fermentation microbial inoculum comprises saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, trichoderma viride and aspergillus niger, and the auxiliary fermentation microbial inoculum comprises lactobacillus plantarum, lactobacillus acidophilus, actinomycetes and photosynthetic bacteria; the culture method of the fermentation inoculum comprises the following steps: s1, slant culture of strain in test tube; s2, carrying out shake flask culture on the strains; s3, performing seed tank expansion culture; s4, culturing in a fermentation tank; s5, preparing a microbial inoculum; s6, drying the bacterial liquid; s7, detecting live bacteria; and S8, subpackaging and packaging.
Description
Technical Field
The invention relates to the technical field of organic fertilizer fermentation, in particular to an organic fertilizer fermentation microbial inoculum and a culture method thereof.
Background
An organic fertilizer strain, i.e. a biological organic fertilizer strain, is a strain for processing organic solid wastes (including organic garbage, straws, livestock and poultry manure, cake meal, agricultural and sideline products and solid wastes generated by food processing) into organic fertilizers after fermentation, deodorization and complete decomposition, an organic fertilizer leaven, i.e. an organic material decomposing agent, can decompose protein, cellulose, hemicellulose, lignin and the like and is formed by compounding bacteria, fungi and the like, the organic fertilizer leaven has high effective viable count and strong degradation capability, and can achieve the effects of warming, deodorizing, eliminating plant diseases and insect pests, weed seeds and improving nutrients, quickly decompose and mineralize carbon, nitrogen, phosphorus, potassium, sulfur and the like in a compost under proper conditions to form simple organic matters, thereby further decomposing the organic fertilizers into nutrient components which can be absorbed by crops The organic fertilizer fermentation method comprises the steps of treating various crop straws (compost and liquid fertilizer), treating kitchen waste (swill), and quickly changing wastes such as melon vines, leaves and weeds, bran vinegar residue, wine residue, vinegar residue, soy sauce residue, bean cakes, residues, powder residue, bean curd residue, bone meal, bagasse and the like into organic fertilizers.
Disclosure of Invention
In order to solve the technical problems, an object of the present invention is to provide an organic fertilizer fermentation inoculant which can accelerate fermentation and decomposition of various organic waste gas, reduce pile turning times, shorten fermentation and decomposition time, and improve practicability;
the invention also aims to provide a culture method of the organic fertilizer fermentation inoculum, which can accelerate the fermentation and decomposition speed of various organic waste gases, reduce the pile turning times, shorten the fermentation and decomposition time and improve the practicability.
The organic fertilizer fermentation microbial inoculum comprises a main fermentation microbial inoculum and an auxiliary fermentation microbial inoculum, wherein the using ratio of the main fermentation microbial inoculum to the auxiliary fermentation microbial inoculum is 4-6: 1, the main fermentation microbial inoculum comprises saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, trichoderma viride and aspergillus niger, and the auxiliary fermentation microbial inoculum comprises lactobacillus plantarum, lactobacillus acidophilus, actinomycetes and photosynthetic bacteria.
Specifically, the organic fertilizer fermentation inoculant further comprises bran powder and protease, the total adding amount of the bran powder and the protease is 5-10 times of that of the main fermentation inoculant and the auxiliary fermentation inoculant, and the using ratio of the bran powder to the protease is 10-12: 1.
Specifically, the addition ratio of each component in the main fermentation inoculant is as follows:
1-2 parts of saccharomyces cerevisiae;
0.8-1.3 parts of bacillus subtilis;
0.5-0.9 parts of bacillus licheniformis;
0.3-0.5 parts of trichoderma viride;
0.2-0.4 part of Aspergillus niger.
Specifically, the addition proportion of each component in the auxiliary fermentation inoculant is as follows:
1-2 parts of lactobacillus plantarum;
1-1.5 parts of lactobacillus acidophilus;
0.6-1.2 parts of actinomycetes;
0.4-0.8 parts of photosynthetic bacteria.
A culture method of an organic fertilizer fermentation inoculant comprises the following steps:
s1, slant culture of strain in test tube: respectively inoculating strains preserved in sand tubes or freeze drying tanks of different strains on different test tube slopes in an aseptic mode for test tube slope culture;
s2, strain shake flask culture: inoculating the strain cultured on the test tube slant to the shake flask culture medium again in an aseptic manner for continuous culture;
s3, seed tank expansion culture: transferring the strain cultured by the shake flask into a seeding tank, and carrying out amplification culture in the seeding tank;
s4, fermentation tank culture: transferring the strain subjected to the enlarged culture to a fermentation tank for fermentation culture to be qualified;
s5, preparation of a microbial inoculum: preparing the strains subjected to gradual diffusion culture into a bacterial liquid, and preparing a zymophyte agent according to a proportion;
s6, drying bacterial liquid: drying the prepared fermentation microbial inoculum in a vacuum freeze drying mode to obtain a dried organic fertilizer fermentation microbial inoculum;
s7, viable bacteria detection: carrying out viable bacteria detection on the dried organic fertilizer fermentation microbial inoculum, and subpackaging the detected qualified microbial inoculum;
s8, subpackaging and packaging: and mixing the detected organic fertilizer fermentation inoculum with bran powder and protease in proportion, and then packaging for storage and transportation.
Specifically, the pH value of the culture medium in the seeding tank is 7.0-7.8, and the culture temperature is 28-32 ℃.
Specifically, at the later stage of the amplification culture, sterile air is introduced into the seeding tank, and is matched with stirring, so that the strains in the seeding tank adapt to the fermentation environment in the fermentation tank.
Specifically, when the viable bacteria are detected, the number of the viable bacteria is more than or equal to 95 hundred million/g, and the viable bacteria are qualified.
Compared with the prior art, the invention has the beneficial effects that: the organic fermentation bacterial manure disclosed by the invention is high in viable count, so that the decomposition speed can be accelerated in the fermentation and decomposition process of organic wastes, the main fermentation microbial inoculum and the auxiliary fermentation microbial inoculum, namely a fermentation microbial inoculum mixture with bran powder and protease, accounting for 0.25-1% of the mass of the agricultural organic wastes to be fermented, are weighed and mixed with the organic wastes uniformly, the water content is adjusted to 40% -50%, and the composting fermentation and decomposition are started;
when the organic fertilizer fermentation microbial inoculum is used for carrying out organic fertilizer fermentation culture, the temperature of a mixture of organic wastes and the microbial inoculum is quickly raised, the pile temperature is quickly raised to 50-60 ℃ within 2 days, fermentation and decomposition can be completed within 5-7 days, pile turning is carried out once every 1-2 days, when odor is gradually dissipated, the materials are not heated any more and loose in shape, and after the materials become dark brown, fermentation is completed;
the organic fertilizer fermented by the method can effectively improve the hardening condition of soil and inhibit the occurrence of plant diseases and insect pests in the soil;
the organic fertilizer fermentation inoculant is simple to operate and wide in application, and can be widely applied to various organic wastes such as various crop straws, livestock and poultry manure, household garbage, agricultural product processing waste and the like;
therefore, when the organic fertilizer fermentation inoculant disclosed by the invention is used for decomposing and fermenting organic wastes, the fermentation and decomposition speeds of various organic waste gases can be accelerated, the pile turning times are reduced, the fermentation and decomposition time is shortened, and the practicability is improved.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
The organic fertilizer fermentation microbial inoculum comprises a main fermentation microbial inoculum and an auxiliary fermentation microbial inoculum, wherein the using ratio of the main fermentation microbial inoculum to the auxiliary fermentation microbial inoculum is 4:1, the main fermentation microbial inoculum comprises saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, trichoderma viride and aspergillus niger, and the auxiliary fermentation microbial inoculum comprises lactobacillus plantarum, lactobacillus acidophilus, actinomycetes and photosynthetic bacteria.
The organic fertilizer fermentation inoculum also comprises bran powder and protease, the total addition amount of the bran powder and the protease is 5 times of the total addition amount of the main fermentation inoculum and the auxiliary fermentation inoculum, and the use ratio of the bran powder to the protease is 10: 1.
Wherein the addition proportion of each component in the main fermentation inoculant is as follows:
1 part of saccharomyces cerevisiae;
1.3 parts of bacillus subtilis;
0.5 part of bacillus licheniformis;
0.4 part of trichoderma viride;
0.2 part of aspergillus niger.
Wherein the addition proportion of each component in the auxiliary fermentation inoculant is as follows:
1 part of lactobacillus plantarum;
1.2 parts of lactobacillus acidophilus;
0.8 part of actinomycetes;
0.5 part of photosynthetic bacteria.
A culture method of an organic fertilizer fermentation inoculant comprises the following steps:
s1, slant culture of strain in test tube: respectively inoculating strains preserved in sand tubes or freeze drying tanks of different strains on different test tube slopes in an aseptic mode for test tube slope culture;
s2, strain shake flask culture: inoculating the strain cultured on the test tube slant to the shake flask culture medium again in an aseptic manner for continuous culture;
s3, seed tank expansion culture: transferring the strain subjected to shake flask culture into a seeding tank, performing amplification culture in the seeding tank, wherein the pH of a culture medium in the seeding tank is 7.4, the culture temperature is 32 ℃, introducing sterile air into the seeding tank in the later period of amplification culture, and stirring to make the strain in the seeding tank adapt to the fermentation environment in a fermentation tank;
s4, fermentation tank culture: transferring the strain subjected to the expanded culture into a fermentation tank for fermentation culture, wherein the viable bacteria number is more than or equal to 95 hundred million/g and is qualified when the viable bacteria are detected;
s5, preparation of a microbial inoculum: preparing the strains subjected to gradual diffusion culture into a bacterial liquid, and preparing a zymophyte agent according to a proportion;
s6, drying bacterial liquid: drying the prepared fermentation microbial inoculum in a vacuum freeze drying mode to obtain a dried organic fertilizer fermentation microbial inoculum;
s7, viable bacteria detection: carrying out viable bacteria detection on the dried organic fertilizer fermentation microbial inoculum, and subpackaging the detected qualified microbial inoculum;
s8, subpackaging and packaging: and mixing the detected organic fertilizer fermentation inoculum with bran powder and protease in proportion, and then packaging for storage and transportation.
Weighing a main fermentation microbial inoculum and an auxiliary fermentation microbial inoculum which are 0.05-0.1% of the mass of agricultural organic waste to be fermented, namely 0.25-1% of a fermentation microbial inoculum mixture with bran powder and protease, uniformly mixing and stirring the mixture with the organic waste, adjusting the water content to 40% -50%, starting composting, fermenting and decomposing, rapidly increasing the temperature of a pile to 50-60 ℃ within 2 days, completing fermenting and decomposing within 5-7 days, turning the pile once every 1-2 days, generating high temperature in the fermentation process, killing harmful bacteria and worm eggs in the organic waste, and completely decomposing the organic waste.
Example 2
The organic fertilizer fermentation microbial inoculum comprises a main fermentation microbial inoculum and an auxiliary fermentation microbial inoculum, wherein the using ratio of the main fermentation microbial inoculum to the auxiliary fermentation microbial inoculum is 6:1, the main fermentation microbial inoculum comprises saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, trichoderma viride and aspergillus niger, and the auxiliary fermentation microbial inoculum comprises lactobacillus plantarum, lactobacillus acidophilus, actinomycetes and photosynthetic bacteria.
The organic fertilizer fermentation inoculum also comprises bran powder and protease, the total addition amount of the bran powder and the protease is 7 times of the total addition amount of the main fermentation inoculum and the auxiliary fermentation inoculum, and the use ratio of the bran powder to the protease is 12: 1.
Wherein the addition proportion of each component in the main fermentation inoculant is as follows:
2 parts of saccharomyces cerevisiae;
1.1 parts of bacillus subtilis;
0.9 part of bacillus licheniformis;
0.3 part of trichoderma viride;
0.3 part of aspergillus niger.
Wherein the addition proportion of each component in the auxiliary fermentation inoculant is as follows:
1.4 parts of lactobacillus plantarum;
1.3 parts of lactobacillus acidophilus;
0.6 part of actinomycetes;
0.7 part of photosynthetic bacteria.
A culture method of an organic fertilizer fermentation inoculant comprises the following steps:
s1, slant culture of strain in test tube: respectively inoculating strains preserved in sand tubes or freeze drying tanks of different strains on different test tube slopes in an aseptic mode for test tube slope culture;
s2, strain shake flask culture: inoculating the strain cultured on the test tube slant to the shake flask culture medium again in an aseptic manner for continuous culture;
s3, seed tank expansion culture: transferring the strain subjected to shake flask culture into a seeding tank, performing amplification culture in the seeding tank, wherein the pH of a culture medium in the seeding tank is 7.6, the culture temperature is 28 ℃, introducing sterile air into the seeding tank in the later period of amplification culture, and stirring to make the strain in the seeding tank adapt to the fermentation environment in a fermentation tank;
s4, fermentation tank culture: transferring the strain subjected to the expanded culture into a fermentation tank for fermentation culture, wherein the viable bacteria number is more than or equal to 95 hundred million/g and is qualified when the viable bacteria are detected;
s5, preparation of a microbial inoculum: preparing the strains subjected to gradual diffusion culture into a bacterial liquid, and preparing a zymophyte agent according to a proportion;
s6, drying bacterial liquid: drying the prepared fermentation microbial inoculum in a vacuum freeze drying mode to obtain a dried organic fertilizer fermentation microbial inoculum;
s7, viable bacteria detection: carrying out viable bacteria detection on the dried organic fertilizer fermentation microbial inoculum, and subpackaging the detected qualified microbial inoculum;
s8, subpackaging and packaging: and mixing the detected organic fertilizer fermentation inoculum with bran powder and protease in proportion, and then packaging for storage and transportation.
Weighing a main fermentation microbial inoculum and an auxiliary fermentation microbial inoculum which are 0.05-0.1% of the mass of agricultural organic waste to be fermented, namely 0.25-1% of a fermentation microbial inoculum mixture with bran powder and protease, uniformly mixing and stirring the mixture with the organic waste, adjusting the water content to 40% -50%, starting composting, fermenting and decomposing, rapidly increasing the temperature of a pile to 50-60 ℃ within 2 days, completing fermenting and decomposing within 5-7 days, turning the pile once every 1-2 days, generating high temperature in the fermentation process, killing harmful bacteria and worm eggs in the organic waste, and completely decomposing the organic waste.
Example 3
The organic fertilizer fermentation microbial inoculum comprises a main fermentation microbial inoculum and an auxiliary fermentation microbial inoculum, wherein the using ratio of the main fermentation microbial inoculum to the auxiliary fermentation microbial inoculum is 5:1, the main fermentation microbial inoculum comprises saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, trichoderma viride and aspergillus niger, and the auxiliary fermentation microbial inoculum comprises lactobacillus plantarum, lactobacillus acidophilus, actinomycetes and photosynthetic bacteria.
The organic fertilizer fermentation inoculum also comprises bran powder and protease, the total addition amount of the bran powder and the protease is 10 times of the total addition amount of the main fermentation inoculum and the auxiliary fermentation inoculum, and the use ratio of the bran powder to the protease is 11: 1.
Wherein the addition proportion of each component in the main fermentation inoculant is as follows:
1.5 parts of saccharomyces cerevisiae;
1.0 part of bacillus subtilis;
0.7 part of bacillus licheniformis;
0.4 part of trichoderma viride;
0.4 part of aspergillus niger.
Wherein the addition proportion of each component in the auxiliary fermentation inoculant is as follows:
2 parts of lactobacillus plantarum;
1 part of lactobacillus acidophilus;
1.0 part of actinomycetes;
0.4 parts of photosynthetic bacteria.
A culture method of an organic fertilizer fermentation inoculant comprises the following steps:
s1, slant culture of strain in test tube: respectively inoculating strains preserved in sand tubes or freeze drying tanks of different strains on different test tube slopes in an aseptic mode for test tube slope culture;
s2, strain shake flask culture: inoculating the strain cultured on the test tube slant to the shake flask culture medium again in an aseptic manner for continuous culture;
s3, seed tank expansion culture: transferring the strain subjected to shake flask culture into a seeding tank, performing amplification culture in the seeding tank, wherein the pH of a culture medium in the seeding tank is 7.8, the culture temperature is 30 ℃, introducing sterile air into the seeding tank in the later period of amplification culture, and stirring to make the strain in the seeding tank adapt to the fermentation environment in a fermentation tank;
s4, fermentation tank culture: transferring the strain subjected to the expanded culture into a fermentation tank for fermentation culture, wherein the viable bacteria number is more than or equal to 95 hundred million/g and is qualified when the viable bacteria are detected;
s5, preparation of a microbial inoculum: preparing the strains subjected to gradual diffusion culture into a bacterial liquid, and preparing a zymophyte agent according to a proportion;
s6, drying bacterial liquid: drying the prepared fermentation microbial inoculum in a vacuum freeze drying mode to obtain a dried organic fertilizer fermentation microbial inoculum;
s7, viable bacteria detection: carrying out viable bacteria detection on the dried organic fertilizer fermentation microbial inoculum, and subpackaging the detected qualified microbial inoculum;
s8, subpackaging and packaging: and mixing the detected organic fertilizer fermentation inoculum with bran powder and protease in proportion, and then packaging for storage and transportation.
Weighing a main fermentation microbial inoculum and an auxiliary fermentation microbial inoculum which are 0.05-0.1% of the mass of agricultural organic waste to be fermented, namely 0.25-1% of a fermentation microbial inoculum mixture with bran powder and protease, uniformly mixing and stirring the mixture with the organic waste, adjusting the water content to 40% -50%, starting composting, fermenting and decomposing, rapidly increasing the temperature of a pile to 50-60 ℃ within 2 days, completing fermenting and decomposing within 5-7 days, turning the pile once every 1-2 days, generating high temperature in the fermentation process, killing harmful bacteria and worm eggs in the organic waste, and completely decomposing the organic waste.
Example 4
The organic fertilizer fermentation microbial inoculum comprises a main fermentation microbial inoculum and an auxiliary fermentation microbial inoculum, wherein the using ratio of the main fermentation microbial inoculum to the auxiliary fermentation microbial inoculum is 5.5:1, the main fermentation microbial inoculum comprises saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, trichoderma viride and aspergillus niger, and the auxiliary fermentation microbial inoculum comprises lactobacillus plantarum, lactobacillus acidophilus, actinomycetes and photosynthetic bacteria.
The organic fertilizer fermentation inoculum also comprises bran powder and protease, the total addition amount of the bran powder and the protease is 8 times of the total addition amount of the main fermentation inoculum and the auxiliary fermentation inoculum, and the use ratio of the bran powder to the protease is 11.5: 1.
Wherein the addition proportion of each component in the main fermentation inoculant is as follows:
1.8 parts of saccharomyces cerevisiae;
0.8 part of bacillus subtilis;
0.8 part of bacillus licheniformis;
0.5 part of trichoderma viride;
0.3 part of aspergillus niger.
Wherein the addition proportion of each component in the auxiliary fermentation inoculant is as follows:
1.7 parts of lactobacillus plantarum;
1.2 parts of lactobacillus acidophilus;
1.2 parts of actinomycetes;
0.8 parts of photosynthetic bacteria.
A culture method of an organic fertilizer fermentation inoculant comprises the following steps:
s1, slant culture of strain in test tube: respectively inoculating strains preserved in sand tubes or freeze drying tanks of different strains on different test tube slopes in an aseptic mode for test tube slope culture;
s2, strain shake flask culture: inoculating the strain cultured on the test tube slant to the shake flask culture medium again in an aseptic manner for continuous culture;
s3, seed tank expansion culture: transferring the strain subjected to shake flask culture into a seeding tank, performing amplification culture in the seeding tank, wherein the pH of a culture medium in the seeding tank is 7.0, the culture temperature is 29 ℃, introducing sterile air into the seeding tank in the later period of amplification culture, and stirring to make the strain in the seeding tank adapt to the fermentation environment in a fermentation tank;
s4, fermentation tank culture: transferring the strain subjected to the expanded culture into a fermentation tank for fermentation culture, wherein the viable bacteria number is more than or equal to 95 hundred million/g and is qualified when the viable bacteria are detected;
s5, preparation of a microbial inoculum: preparing the strains subjected to gradual diffusion culture into a bacterial liquid, and preparing a zymophyte agent according to a proportion;
s6, drying bacterial liquid: drying the prepared fermentation microbial inoculum in a vacuum freeze drying mode to obtain a dried organic fertilizer fermentation microbial inoculum;
s7, viable bacteria detection: carrying out viable bacteria detection on the dried organic fertilizer fermentation microbial inoculum, and subpackaging the detected qualified microbial inoculum;
s8, subpackaging and packaging: and mixing the detected organic fertilizer fermentation inoculum with bran powder and protease in proportion, and then packaging for storage and transportation.
Weighing a main fermentation microbial inoculum and an auxiliary fermentation microbial inoculum which are 0.05-0.1% of the mass of agricultural organic waste to be fermented, namely 0.25-1% of a fermentation microbial inoculum mixture with bran powder and protease, uniformly mixing and stirring the mixture with the organic waste, adjusting the water content to 40% -50%, starting composting, fermenting and decomposing, rapidly increasing the temperature of a pile to 50-60 ℃ within 2 days, completing fermenting and decomposing within 5-7 days, turning the pile once every 1-2 days, generating high temperature in the fermentation process, killing harmful bacteria and worm eggs in the organic waste, and completely decomposing the organic waste.
The organic fermentation bacterial manure disclosed by the invention is high in viable count, so that the decomposition speed can be accelerated in the fermentation and decomposition process of organic wastes, the main fermentation microbial inoculum and the auxiliary fermentation microbial inoculum, namely a fermentation microbial inoculum mixture with bran powder and protease, accounting for 0.25-1% of the mass of the agricultural organic wastes to be fermented, are weighed and mixed with the organic wastes uniformly, the water content is adjusted to 40% -50%, and the composting fermentation and decomposition are started;
when the organic fertilizer fermentation microbial inoculum is used for carrying out organic fertilizer fermentation culture, the temperature of a mixture of organic wastes and the microbial inoculum is quickly raised, the pile temperature is quickly raised to 50-60 ℃ within 2 days, fermentation and decomposition can be completed within 5-7 days, pile turning is carried out once every 1-2 days, when odor is gradually dissipated, the materials are not heated any more and loose in shape, and after the materials become dark brown, fermentation is completed;
the organic fertilizer fermented by the method can effectively improve the hardening condition of soil and inhibit the occurrence of plant diseases and insect pests in the soil;
the organic fertilizer fermentation inoculant is simple to operate and wide in application, and can be widely applied to various organic wastes such as various crop straws, livestock and poultry manure, household garbage, agricultural product processing waste and the like;
therefore, when the organic fertilizer fermentation inoculant disclosed by the invention is used for decomposing and fermenting organic wastes, the fermentation and decomposition speeds of various organic waste gases can be accelerated, the pile turning times are reduced, the fermentation and decomposition time is shortened, and the practicability is improved.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (8)
1. An organic fertilizer fermentation bacterial agent is characterized by comprising a main fermentation bacterial agent and an auxiliary fermentation bacterial agent, wherein the using ratio of the main fermentation bacterial agent to the auxiliary fermentation bacterial agent is 4-6: 1, the main fermentation bacterial agent comprises saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, trichoderma viride and aspergillus niger, and the auxiliary fermentation bacterial agent comprises lactobacillus plantarum, lactobacillus acidophilus, actinomycetes and photosynthetic bacteria.
2. The organic fertilizer fermentation inoculant according to claim 1, further comprising bran powder and protease, wherein the total amount of the bran powder and the protease is 5-10 times of the total amount of the main fermentation inoculant and the auxiliary fermentation inoculant, and the ratio of the bran powder to the protease is 10-12: 1.
3. The organic fertilizer fermentation inoculant according to claim 1, wherein the main fermentation inoculant comprises the following components in the addition ratio:
1-2 parts of saccharomyces cerevisiae;
0.8-1.3 parts of bacillus subtilis;
0.5-0.9 parts of bacillus licheniformis;
0.3-0.5 parts of trichoderma viride;
0.2-0.4 part of Aspergillus niger.
4. The organic fertilizer fermentation inoculant according to claim 1, wherein the auxiliary fermentation inoculant comprises the following components in proportion:
1-2 parts of lactobacillus plantarum;
1-1.5 parts of lactobacillus acidophilus;
0.6-1.2 parts of actinomycetes;
0.4-0.8 parts of photosynthetic bacteria.
5. The method for culturing the organic fertilizer fermentation inoculum as claimed in any one of claims 2 to 4, which is characterized by comprising the following steps:
s1, slant culture of strain in test tube: respectively inoculating strains preserved in sand tubes or freeze drying tanks of different strains on different test tube slopes in an aseptic mode for test tube slope culture;
s2, strain shake flask culture: inoculating the strain cultured on the test tube slant to the shake flask culture medium again in an aseptic manner for continuous culture;
s3, seed tank expansion culture: transferring the strain cultured by the shake flask into a seeding tank, and carrying out amplification culture in the seeding tank;
s4, fermentation tank culture: transferring the strain subjected to the enlarged culture to a fermentation tank for fermentation culture to be qualified;
s5, preparation of a microbial inoculum: preparing the strains subjected to gradual diffusion culture into a bacterial liquid, and preparing a zymophyte agent according to a proportion;
s6, drying bacterial liquid: drying the prepared fermentation microbial inoculum in a vacuum freeze drying mode to obtain a dried organic fertilizer fermentation microbial inoculum;
s7, viable bacteria detection: carrying out viable bacteria detection on the dried organic fertilizer fermentation microbial inoculum, and subpackaging the detected qualified microbial inoculum;
s8, subpackaging and packaging: and mixing the detected organic fertilizer fermentation inoculum with bran powder and protease in proportion, and then packaging for storage and transportation.
6. The method for culturing the organic fertilizer fermentation inoculant according to claim 5, wherein the pH of the culture medium in the seeding tank is 7.0-7.8, and the culture temperature is 28-32 ℃.
7. The method for culturing an organic fertilizer fermentation inoculant according to claim 5, wherein in the later stage of the expanded culture, sterile air is introduced into the seed tank, and the strain in the seed tank is adapted to the fermentation environment in the fermentation tank by stirring.
8. The method for culturing the organic fertilizer fermentation inoculum according to claim 5, wherein the number of the viable bacteria is not less than 95 hundred million/g, and the viable bacteria is qualified when being detected.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113135777A (en) * | 2021-04-22 | 2021-07-20 | 广西壮族自治区农业科学院 | On-site harmless treatment method for fruit and vegetable wastes |
| CN114409477A (en) * | 2022-02-16 | 2022-04-29 | 深圳市淳睿科技发展有限公司 | Organic fertilizer and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441360A (en) * | 2015-12-27 | 2016-03-30 | 陈景河 | Organic fertilizer fermentation inoculant |
| CN105733975A (en) * | 2014-12-12 | 2016-07-06 | 河北省农林科学院遗传生理研究所 | Multifunctional high efficient organic material decomposition agent and preparation method thereof |
| CN109081757A (en) * | 2018-08-14 | 2018-12-25 | 深圳市三林生物科技工程有限公司 | Biological organic fertilizer and preparation method thereof |
-
2021
- 2021-01-22 CN CN202110085661.9A patent/CN112662593A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105733975A (en) * | 2014-12-12 | 2016-07-06 | 河北省农林科学院遗传生理研究所 | Multifunctional high efficient organic material decomposition agent and preparation method thereof |
| CN105441360A (en) * | 2015-12-27 | 2016-03-30 | 陈景河 | Organic fertilizer fermentation inoculant |
| CN109081757A (en) * | 2018-08-14 | 2018-12-25 | 深圳市三林生物科技工程有限公司 | Biological organic fertilizer and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113135777A (en) * | 2021-04-22 | 2021-07-20 | 广西壮族自治区农业科学院 | On-site harmless treatment method for fruit and vegetable wastes |
| CN114409477A (en) * | 2022-02-16 | 2022-04-29 | 深圳市淳睿科技发展有限公司 | Organic fertilizer and preparation method thereof |
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