CN112646720B - Research and development device for mutant small molecule anticancer drugs - Google Patents

Research and development device for mutant small molecule anticancer drugs Download PDF

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Publication number
CN112646720B
CN112646720B CN202110017157.5A CN202110017157A CN112646720B CN 112646720 B CN112646720 B CN 112646720B CN 202110017157 A CN202110017157 A CN 202110017157A CN 112646720 B CN112646720 B CN 112646720B
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cavity
wall surface
detection
end surface
cancer cell
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CN112646720A (en
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邹黎
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Dongguan Lanwei Medical Laboratory Co ltd
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Dongguan Lanwei Medical Laboratory Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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Abstract

The invention discloses a research and development device for mutant micromolecule anticancer drugs, which comprises a detection box, wherein a working cavity is arranged in the detection box, a cancer cell culture bottle extending upwards is fixedly arranged on the lower wall surface of the left side of the working cavity, a cancer cell culture cavity with an upward opening is arranged on the upper end surface of the cancer cell culture bottle, and cancer cells cultured at a constant temperature can be stored in the cancer cell culture cavity; according to the invention, the cancer cell culture bottle is sealed and cultured to provide a proper living environment for the cancer cells, the liftable cancer cell suction device is utilized to suck the cancer cells and maintain the stability of the living environment of the cancer cells, the cells on the outer wall of the suction tube can be cleaned in time, and meanwhile, the device can detect the temperature of the culture dish and compare the temperature of the cancer cell culture bottle through the liquid medicine to control the release of the cancer cells in the suction tube, so that the influence of the environment change on the accuracy of an experimental result is effectively avoided.

Description

Research and development device for mutant small molecule anticancer drugs
Technical Field
The invention relates to the technical field of drug research and development, in particular to a research and development device for mutant small molecule anticancer drugs.
Background
Need detect the property of a medicine of medicine in the anticancer drug research and development process, traditional medicine detects and goes on in the laboratory, the suitable living environment of cancer cell that provides that can not be fine, need go on under specific environment to cancer cell's cultivation, need reduce the influence of external environment change, current cancer cell shifts and carries out the auxiliary operation through utilizing the suction tube, the suction tube outer wall causes cancer cell to remain, and then cause the experimental data deviation easily, need timely to clear up the suction tube outer wall simultaneously, the experimental step is too loaded down with trivial details, influence experiment operation convenience.
Disclosure of Invention
The invention aims to provide a research and development device for mutant small-molecule anticancer drugs, and solves the problem that experimental results are inaccurate due to environmental changes.
The invention is realized by the following technical scheme.
The invention relates to a research and development device for mutant small-molecule anticancer drugs, which comprises a detection box, wherein a working cavity is arranged in the detection box, an upwardly extending cancer cell culture bottle is fixedly arranged on the lower wall surface of the left side of the working cavity, a cancer cell culture cavity with an upward opening is arranged on the upper end surface of the cancer cell culture bottle, cancer cells cultured at a constant temperature can be stored in the cancer cell culture cavity, a closed cover plate is fixedly arranged on the upper end surface of the cancer cell culture cavity, the cancer cell culture cavity can be closed by the closed cover plate, an extraction hole is communicated between the upper end surface and the lower end surface of the closed cover plate, a wiping cavity extending to one side away from the circle center of the closed cover plate is communicated with the inner wall surface of the upper end of the extraction hole, an annular wiping ring is fixedly arranged in the wiping cavity, the wiping ring is made of a memory material capable of deforming, four annular array closed cavities are communicated with the inner wall surface of the extraction hole on the lower side of the wiping ring, the upper wall surface of the closed cavity is communicated with a sliding guide cavity extending upwards, a closed vertebral lamina capable of moving along one side close to or far away from the circle center of the closed cover plate is arranged in the closed cavity, the four closed vertebral laminas can seal the extraction hole, a guide slider is fixedly arranged on the upper end surface of the closed vertebral lamina and has a sliding guide effect, one end, far away from the axle center of the closed cover plate, of the guide slider can extend to the sliding guide cavity, the guide slider can slide in the sliding guide cavity, a closed spring is fixedly arranged between the end surface, far away from the axle center of the closed cover plate, of the closed vertebral lamina and the wall surface, far away from the closed cover plate, of the closed cavity, a translation motor is fixedly arranged in the detection box at the upper end of the left side of the working cavity, and the right end surface of the translation motor is dynamically connected with a translation screw rod penetrating through the left wall surface and the right wall surface of the working cavity, an extraction machine body capable of moving left and right is matched with the outer circular surface of the translation screw rod on the upper side of the cancer cell culture bottle in a threaded manner, an absorption device storage cavity is arranged in the extraction machine body, an absorption tube extension hole is communicated and arranged between the lower wall surface of the absorption device storage cavity and the lower end surface of the extraction machine body, a support table is fixedly arranged on the lower wall surface of the working cavity on the right side of the cancer cell culture bottle, a temperature contrast detection mechanism is arranged in the support table, a liquid medicine detection culture dish is fixedly arranged on the upper end surface of the support table, a liquid medicine culture cavity with an upward opening is communicated and arranged on the upper end surface of the liquid medicine detection culture dish, liquid medicine to be tested can be stored in the liquid medicine culture cavity, a temperature detection cavity is arranged in the liquid medicine detection culture dish on the lower side of the liquid medicine culture dish, and the temperature detection cavity can sense the temperature in the liquid medicine detection culture dish, the cancer cell culture chamber downside cancer cell culture bottle inside is equipped with the blake bottle and detects the chamber, the blake bottle detects the chamber and can real-time detection cancer cell culture intracavity portion temperature.
Preferably, the working chamber upside be equipped with the translation sliding chamber in the detection case, translation sliding chamber lower wall with the intercommunication is equipped with the translation sliding tray between the working chamber upper wall, with the extraction organism corresponds the position translation sliding intracavity portion is equipped with the slide that leads that can the horizontal slip, lead the slide under the fixed being equipped with of terminal surface and pass translation sliding tray and extend to the inside connecting rod of working chamber, the connecting rod lower extreme with extraction organism up end fixed connection.
Preferably, the suction device deposits the left side in chamber be equipped with rack lift chamber in the extractor, rack lift chamber rear wall intercommunication is equipped with the lift guide chute of ranging back, rack lift chamber right side wall with the suction device deposits and communicates between the intracavity face and be equipped with the drag link shifting chute, with the left side that the suction device deposited the chamber intermediate position and corresponds the extractor internal fixation is equipped with elevator motor, elevator motor right-hand member face power is connected with extends to the gear shaft in the rack lift chamber, the gear shaft right-hand member is fixed and is equipped with the gear.
Preferably, a rack capable of sliding up and down is arranged on the rear side of the gear, the outer circular surface of the gear is meshed with the front end surface of the rack, a lifting guide slide rod extending into the lifting guide slide groove is fixedly arranged on the rear end of the rack, the rear end of the lifting guide slide rod can slide up and down in the lifting guide slide groove, a cross pull rod penetrating through the cross pull rod moving groove and extending into the storage cavity of the suction device is fixedly arranged on the right end surface of the lower side of the rack, an annular suction tube fixing ring is fixedly arranged on the right end of the cross pull rod, a vertically placed suction tube is fixedly arranged on the inner circular surface of the suction tube fixing ring, a suction tube rubber head communicated with each other is fixedly arranged on the upper end of the suction tube, upper extrusion blocks which are mutually symmetrical are fixedly arranged on the front and rear wall surfaces of the storage cavity of the suction device on the upper side of the suction tube rubber head, and the suction tube rubber head can be extruded when continuously moving up, the front wall and the rear wall of the storage cavity of the suction device on the lower side of the upper extrusion block are fixedly provided with lower extrusion blocks which are mutually symmetrical, and the rubber head of the suction pipe can be extruded by the lower extrusion blocks when moving to the lower limit.
Preferably, a temperature detection and regulation cavity is arranged in the support platform at the lower side of the temperature detection cavity, the left end and the right end of the temperature detection and regulation cavity are both fixedly provided with temperature guide plates which are symmetrical with each other, the heat conducting plate can transfer heat, the inner wall surface of the temperature detection regulation cavity is provided with a detection plate capable of sliding left and right, the temperature detection and control cavities on the left side and the right side of the detection plate are filled with inert gases with the same quality and quantity, a culture bottle detection pipeline is communicated between the lower wall surface of the culture bottle detection cavity and the left end surface of the left temperature conduction plate, the temperature detects wall and right side under the chamber the intercommunication is equipped with the temperature and detects the conveyer pipe between leading the temperature board right-hand member face, is close to temperature detects regulation and control chamber one side the temperature detects the conveyer pipe lower extreme and is equipped with the chamber that opens and shuts, open and shut chamber upper wall with the intercommunication is equipped with the switching passageway between the temperature detects the conveyer pipe.
Preferably, a trigger block extending leftwards is fixedly arranged on the right wall surface of the working cavity at the position corresponding to the lower end of the extraction machine body, a trigger cavity is arranged in the trigger block, a trigger plate capable of moving left and right is arranged on the inner wall surface of the trigger cavity, a trigger rod extending out of the left end surface of the trigger block is fixedly arranged on the left end surface of the trigger plate, a trigger spring is fixedly arranged between the right end surface of the trigger plate and the right wall surface of the trigger cavity, an opening and closing plate capable of sliding up and down is arranged on the inner wall surface of the opening and closing cavity, an opening and closing lifting rod capable of sliding up and down in the opening and closing channel is fixedly arranged on the upper end surface of the opening and closing plate, the opening and closing lifting rod can seal the temperature detection conveying pipe and insulate the gas in the temperature detection cavity, an opening and closing spring is fixedly arranged between the lower end face of the opening and closing plate and the lower wall face of the opening and closing cavity, and a pull rope is fixedly connected between the lower end face of the opening and closing plate and the right end face of the trigger plate.
Preferably, wall intermediate position intercommunication is equipped with downwardly extending's release and triggers the chamber under the temperature detects regulation and control chamber, release triggers intracavity internal face and is equipped with the release trigger head that can slide from top to bottom, the fixed release signal piece that is equipped with of wall under the chamber is triggered in the release, release trigger head down the terminal surface with release triggers fixedly to be equipped with the release signal spring under the chamber between the wall, release trigger head with can control when the release signal piece contacts elevator motor drive and drive the rack continues upward movement one section distance to accomplish the extrusion of suction tube rubber head.
The invention has the beneficial effects that: according to the device, the cancer cell culture bottle is sealed and cultured, a proper living environment is provided for the cancer cells, the cancer cell suction device capable of lifting is utilized, the stability of the living environment of the cancer cells can be maintained while the cancer cells are sucked, the cells on the outer wall of the suction tube can be cleaned in time, meanwhile, the device can detect the temperature of the culture dish and the temperature of the cancer cell culture bottle through the liquid medicine and compare the temperature with the temperature of the cancer cell culture bottle, the release of the cancer cells in the suction tube is controlled, and the influence of environmental change on the accuracy of an experimental result is effectively avoided.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a schematic structural diagram at A-A in FIG. 1 according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram at B-B in FIG. 1 according to an embodiment of the present invention;
FIG. 4 is an enlarged schematic view of the embodiment of the present invention at C in FIG. 1;
FIG. 5 is a schematic structural diagram at D-D in FIG. 4 according to an embodiment of the present invention;
FIG. 6 is a schematic diagram of a structure at E-E in FIG. 4 according to an embodiment of the present invention;
FIG. 7 is an enlarged view of the embodiment of the present invention shown in FIG. 1 at F.
Detailed Description
The invention will now be described in detail with reference to fig. 1-7, wherein for ease of description the orientations described below are now defined as follows: the up, down, left, right, and front-back directions described below coincide with the up, down, left, right, and front-back directions in the projection relation of fig. 1 itself.
The research and development device for mutant small-molecule anticancer drugs, which is described in conjunction with the accompanying drawings 1-7, comprises a detection box 11, a working chamber 12 is arranged inside the detection box 11, a cancer cell culture bottle 15 extending upwards is fixedly arranged on the lower wall surface of the left side of the working chamber 12, a cancer cell culture chamber 16 with an upward opening is arranged on the upper end surface of the cancer cell culture bottle 15, cancer cells cultured at a constant temperature can be stored in the cancer cell culture chamber 16, a sealing cover plate 17 is fixedly arranged on the upper end surface of the cancer cell culture chamber 16, the sealing cover plate 17 can seal the cancer cell culture chamber 16, an extraction hole 18 is communicated between the upper end surface and the lower end surface of the sealing cover plate 17, a wiping chamber 21 extending to one side far away from the circle center of the sealing cover plate 17 is communicated with the inner wall surface of the upper end of the extraction hole 18, an annular wiping ring 22 is fixedly arranged inside the wiping chamber 21, and the wiping ring 22 is a memory material capable of deforming, the inner wall surface of the extraction hole 18 on the lower side of the wiping ring 22 is communicated with four annular array closed cavities 19, the upper wall surface of the closed cavity 19 is communicated with a sliding guide cavity 20 which extends upwards, a closed vertebral plate 23 which can move along one side close to or far away from the circle center of the closed cover plate 17 is arranged in the closed cavity 19, the four closed vertebral plates 23 can seal the extraction hole 18, a guide slider 71 is fixedly arranged on the upper end surface of the closed vertebral plate 23, the guide slider 71 has a sliding guide function, one end, away from the axis center of the closed cover plate 17, of the guide slider 71 can extend to the sliding guide cavity 20, the guide slider 71 can slide in the sliding guide cavity 20, the closed vertebral plate 23 is far away from one side end surface of the axis of the closed cover plate 17 and the same side, a closed spring 24 is fixedly arranged between the wall surface of the closed cavity 19 far away from the side of the closed cover plate 17, and a translation motor 25 is fixedly arranged in the detection box 11 at the upper end of the left side of the working cavity 12, the right end face of the translation motor 25 is in power connection with a translation screw 26 penetrating through the left and right wall faces of the working chamber 12, an extraction body 27 capable of moving left and right is in threaded fit with the outer circular face of the translation screw 26 positioned on the upper side of the cancer cell culture bottle 15, an extraction device storage chamber 30 is arranged inside the extraction body 27, an extraction tube extension hole 31 is communicated and arranged between the lower wall face of the extraction device storage chamber 30 and the lower end face of the extraction body 27, a support table 53 is fixedly arranged on the lower wall face of the working chamber 12 on the right side of the cancer cell culture bottle 15, a temperature contrast detection mechanism is arranged inside the support table 53, a liquid medicine detection culture dish 54 is fixedly arranged on the upper end face of the support table 53, a liquid medicine culture chamber 70 with an upward opening is communicated and arranged on the upper end face of the liquid medicine detection culture dish 54, liquid medicine to be tested can be stored in the liquid medicine culture chamber 70, and a temperature detection chamber 55 is arranged in the liquid medicine detection culture dish 54 on the lower side of the liquid medicine culture chamber 70, temperature detection chamber 55 can respond to the inside temperature of liquid medicine detection culture dish 54, cancer cell culture chamber 16 downside cancer cell culture bottle 15 is inside to be equipped with blake bottle and to detect chamber 51, blake bottle detects chamber 51 and can real-time detection cancer cell culture chamber 16 inside temperature.
Beneficially, a translational sliding cavity 14 is arranged in the detection box 11 on the upper side of the working cavity 12, a translational sliding groove 13 is arranged between the lower wall surface of the translational sliding cavity 14 and the upper wall surface of the working cavity 12 in a communicating manner, a guide sliding plate 29 capable of sliding left and right is arranged inside the translational sliding cavity 14 at a position corresponding to the extraction machine body 27, a connecting rod 28 penetrating through the translational sliding groove 13 and extending into the working cavity 12 is fixedly arranged on the lower end surface of the guide sliding plate 29, and the lower end of the connecting rod 28 is fixedly connected with the upper end surface of the extraction machine body 27.
Beneficially, a rack lifting cavity 33 is arranged in the extraction machine body 27 on the left side of the suction device storage cavity 30, a lifting guide chute 34 extending backwards is arranged on the rear wall surface of the rack lifting cavity 33 in a communicating manner, a tie rod moving chute 35 is arranged between the right wall surface of the rack lifting cavity 33 and the inner wall surface of the suction device storage cavity 30 in a communicating manner, a lifting motor 32 is fixedly arranged in the extraction machine body 27 on the left side corresponding to the middle position of the suction device storage cavity 30, a gear shaft 36 extending into the rack lifting cavity 33 is connected to the right end surface of the lifting motor 32 in a power mode, and a gear 37 is fixedly arranged at the right end of the gear shaft 36.
Beneficially, a rack 38 capable of sliding up and down is arranged on the rear side of the gear 37, the outer circumferential surface of the gear 37 is engaged with the front end surface of the rack 38, a lifting guide slide rod 39 extending into the lifting guide slide groove 34 is fixedly arranged on the rear end of the rack 38, the rear end of the lifting guide slide rod 39 can slide up and down in the lifting guide slide groove 34, a tie rod 40 penetrating through the tie rod moving groove 35 and extending into the suction device storage cavity 30 is fixedly arranged on the right end surface of the lower side of the rack 38, an annular suction tube fixing ring 41 is fixedly arranged on the right end of the tie rod 40, a vertically arranged suction tube 42 is fixedly arranged on the inner circumferential surface of the suction tube fixing ring 41, mutually communicated suction tube rubber heads 43 are fixedly arranged on the upper ends of the suction tubes 42, mutually symmetrical upper extrusion blocks 44 are fixedly arranged on the front and rear wall surfaces of the suction device storage cavity 30 on the upper side of the suction tube rubber heads 43, when the suction pipe rubber head 43 moves upwards continuously, the suction pipe rubber head 43 can be extruded, the front wall surface and the rear wall surface of the suction device storage cavity 30 on the lower side of the upper extrusion block 44 are fixedly provided with lower extrusion blocks 45 which are symmetrical with each other, and the suction pipe rubber head 43 can be extruded by the lower extrusion blocks 45 when moving to the lower limit.
Advantageously, a temperature detection and regulation cavity 57 is arranged in the support platform 53 at the lower side of the temperature detection cavity 55, the left end and the right end of the temperature detection and regulation cavity 57 are both fixedly provided with temperature guide plates 58 which are mutually symmetrical, the temperature guide plate 58 can transmit heat, the inner wall surface of the temperature detection and regulation cavity 57 is provided with a detection plate 59 capable of sliding left and right, the temperature detection and control cavities 57 on the left and right sides of the detection plate 59 are filled with inert gas with the same quality, a culture bottle detection pipeline 52 is communicated between the lower wall surface of the culture bottle detection cavity 51 and the left end surface of the temperature guide plate 58 on the left side, a temperature detection conveying pipe 56 is communicated between the lower wall surface of the temperature detection cavity 55 and the right end surface of the temperature guide plate 58 on the right side, an open-close cavity 64 is arranged at the lower end of the temperature detection conveying pipe 56 on one side close to the temperature detection regulating cavity 57, an open/close passage 65 is provided between the upper wall surface of the open/close chamber 64 and the temperature detection duct 56.
Beneficially, a trigger block 46 extending leftward is fixedly arranged on the right wall surface of the working chamber 12 at a position corresponding to the lower end of the extraction machine body 27, a trigger chamber 47 is arranged inside the trigger block 46, a trigger plate 48 capable of moving left and right is arranged on the inner wall surface of the trigger chamber 47, a trigger rod 49 extending out of the left end surface of the trigger block 46 is fixedly arranged on the left end surface of the trigger plate 48, a trigger spring 50 is fixedly arranged between the right end surface of the trigger plate 48 and the right wall surface of the trigger chamber 47, an opening and closing plate 66 capable of sliding up and down is arranged on the inner wall surface of the opening and closing chamber 64, an opening and closing lifting rod 67 capable of sliding up and down in the opening and closing channel 65 is fixedly arranged on the upper end surface of the opening and closing plate 66, the opening and closing lifting rod 67 can close the temperature detection delivery pipe 56 and insulate the gas inside the temperature detection chamber 55, and an opening and closing spring 68 is fixedly arranged between the lower end surface of the opening and closing plate 66 and the lower wall surface of the opening and closing chamber 64, a draw cord 69 is fixedly connected between the lower end surface of the opening/closing plate 66 and the right end surface of the trigger plate 48.
Beneficially, a release trigger cavity 60 extending downward is communicated with the middle position of the lower wall surface of the temperature detection and control cavity 57, a release trigger head 61 capable of sliding up and down is arranged on the inner wall surface of the release trigger cavity 60, a release signal block 62 is fixedly arranged on the lower wall surface of the release trigger cavity 60, a release signal spring 63 is fixedly arranged between the lower end surface of the release trigger head 61 and the lower wall surface of the release trigger cavity 60, and when the release trigger head 61 is in contact with the release signal block 62, the lift motor 32 can be controlled to drive and drive the rack 38 to move upwards for a certain distance to complete the extrusion of the suction pipe rubber head 43.
In the initial state, the extraction body 27 is positioned above the cancer cell culture bottle 15, the closed vertebral plate 23 closes the extraction hole 18, the rubber head 43 of the aspiration tube and the upper squeezing block 44 abut against each other, the rubber head 43 of the aspiration tube is in the stretching state, the trigger plate 48 and the trigger rod 49 are in the left limit position, the release trigger head 61 is in the upper limit position, the release trigger head 61 and the release signal block 62 are out of contact, the opening and closing plate 66 and the opening and closing lifting rod 67 are in the upper limit position, the opening and closing lifting rod 67 closes the temperature detection delivery pipe 56, and the detection plate 59 is in the right limit position.
During operation, the lifting motor 32 is driven to rotate forward, so as to drive the gear shaft 36 to rotate, further drive the gear 37 to rotate, further drive the rack 38 and the lifting guide rod 39 to move downward, further drive the suction tube fixing ring 41 and the suction tube 42 to move downward integrally through the tie rod 40, further drive the suction tube rubber head 43 to move downward together, extend the lower end of the suction tube 42 into the suction hole 18 and extrude the wiping ring 22 to deform, the suction tube 42 moves downward continuously and abuts against the end surface of the guide block 71, further push the guide block 71 to slide in the guide sliding cavity 20 to one side far away from the axis of the closed cover plate 17, further drive the closed vertebral plate 23 to compress the closed spring 24 to move to one side far away from the axis of the closed cover plate 17, further open the suction hole 18 through the closed vertebral plate 23, further move the suction tube 42 downward and extend into the cancer cell culture cavity 16, further drive the suction tube rubber head 43 to move downward continuously through the lower extrusion block 45, and complete extrusion of the suction tube rubber head 43, further pressing the gas inside the rubber head 43 into the cancer cell culture chamber 16, extending the lower end of the suction tube 42 into the cancer cells inside the cancer cell culture chamber 16, driving the elevator motor 32 to rotate reversely, further driving the gear shaft 36 to rotate reversely, further driving the gear 37 to rotate reversely, further driving the rack 38 engaged with each other to move upwards, further driving the suction tube 42 and the rubber head 43 of the suction tube to move upwards together, when the lower extrusion block 45 is separated from the extrusion to the rubber head 43 of the suction tube, starting to suck air inside the rubber head 43 of the suction tube, further sucking the cancer cells inside the cancer cell culture chamber 16 into the suction tube 42, further continuing to drive the suction tube 42 and the rubber head 43 of the suction tube to move upwards, when the outer circular surface of the suction tube 42 is separated from the vertebral plate 23, the closed vertebral plate 23 closes the suction hole 18 again under the elastic force of the closing spring 24, further ensuring the sealing inside the cancer cell culture chamber 16, when the suction tube 42 moves upwards continuously, the outer circular surface of the suction tube 42 contacts with the wiping ring 22, and the wiping ring 22 wipes the cancer cells adhered to the outer circular surface of the suction tube 42, so as to ensure the cleanness of the outer circular surface of the suction tube 42, the suction tube 42 and the suction tube rubber head 43 move upwards continuously, when the suction tube rubber head 43 abuts against the upper extrusion block 44, the lifting motor 32 is stopped being driven, so as to keep the suction tube 42 and the suction tube rubber head 43 still, at this time, the translation motor 25 is driven to rotate in the forward direction, the translation screw 26 is driven to rotate, the whole extraction body 27 is driven to move rightwards under the guiding slide of the connecting rod 28 and the guide plate 29, when the extraction body 27 moves rightwards, the trigger plate 48 is pushed by the trigger rod 49 to compress the trigger spring 50 to move rightwards continuously, when the extraction body 27 moves to the right limit, the translation motor 25 is stopped being driven, at this time, the suction tube 42 is positioned at the upper side of the liquid medicine culture chamber 70, when the trigger plate 48 moves to the right limit, the opening and closing plate 66 is pulled by the pull rope 69 to compress the opening and closing spring 68 to move downwards, so that the opening and closing lifting rod 67 is driven to move downwards and the sealing of the temperature detection conveying pipe 56 is released, at the moment, the temperature inside the temperature detection cavity 55 is contacted with the right temperature guide plate 58 through the temperature detection conveying pipe 56, the inert gas on the right side of the detection plate 59 is heated through the right temperature guide plate 58, if the detection temperature inside the culture bottle detection cavity 51 is the same as the temperature inside the temperature detection cavity 55, the gas pressures on the left side and the right side of the detection plate 59 are gradually balanced, so that the detection plate 59 is pushed to move leftwards and stop at the middle position of the temperature detection regulation and control cavity 57, at the moment, the detection plate 59 pushes the release trigger head 61 to compress the release signal spring 63 to move downwards, so that the contact between the release trigger head 61 and the release signal block 62 is completed, so that the lifting motor 32 can be driven to rotate reversely and continue to drive the rack 38 to move upwards for a certain distance, then the rubber head 43 of the suction tube and the upper extrusion block 44 continue to extrude, so as to discharge the cancer cells in the suction tube 42 into the liquid medicine culture chamber 70, and further complete the effectiveness test of the liquid medicine on the cancer cells, the translation motor 25 is driven to rotate reversely, so as to drive the translation screw 26 to rotate reversely, and further drive the extraction machine body 27 to move leftward and reset, at this time, the extraction machine body 27 is disconnected from extruding the trigger rod 49, and further the trigger plate 48 and the trigger rod 49 move leftward and reset under the action of the elastic force of the trigger spring 50, the opening and closing plate 66 moves upward and resets under the action of the elastic force of the opening and closing spring 68, and further the opening and closing lifting rod 67 moves upward and seals the temperature detection delivery pipe 56, after a period of time, the right temperature guide plate 58 stops thermal delivery, and further the inert gas in the temperature detection and control chamber 57 at the left side of the detection plate 59 forms a temperature difference with the gas in the temperature detection and control chamber 57 at the right side of the detection plate 59, and then the detection plate 59 is pushed to move rightwards, and the trigger head 61 is released to move upwards and reset under the elasticity of the release signal spring 63, at the moment, the lifting motor 32 rotates forwards and continues to drive the rack 38 to move downwards to the initial position, and then the overall reset of the equipment is completed.
The above embodiments are only for illustrating the technical idea and features of the present invention, and the purpose of the present invention is to enable those skilled in the art to understand the content of the present invention and implement the present invention, and not to limit the protection scope of the present invention by this. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims (1)

1. The utility model provides a research and development device to mutant small molecule anticancer drugs, includes the detection case, its characterized in that: the detection box is internally provided with a working chamber, the lower wall surface of the left side of the working chamber is fixedly provided with an upwardly extending cancer cell culture bottle, the upper end surface of the cancer cell culture bottle is provided with a cancer cell culture chamber with an upward opening, the cancer cell culture chamber can store cancer cells cultured at constant temperature, the upper end surface of the cancer cell culture chamber is fixedly provided with a sealing cover plate, the sealing cover plate can seal the cancer cell culture chamber, an extraction hole is communicated between the upper end surface and the lower end surface of the sealing cover plate, the inner wall surface of the upper end of the extraction hole is communicated with a wiping chamber extending to one side far away from the circle center of the sealing cover plate, the inner part of the wiping chamber is fixedly provided with an annular wiping ring, the wiping ring is made of a memory material capable of deforming, the inner wall surface of the extraction hole at the lower side of the wiping ring is communicated with four annular array sealing chambers, and the upper wall surface of the sealing chamber is communicated with an upwardly extending sliding guide chamber, the closed cavity is internally provided with a closed vertebral lamina which can move along one side close to or far away from the circle center of the closed cover plate, the four closed vertebral laminas can close the extraction hole, the upper end surface of the closed vertebral lamina is fixedly provided with a guide block, the guide block has a guide sliding effect, one end of the guide block, far away from the axle center of the closed cover plate, can extend to the guide sliding cavity, the guide block can slide in the guide sliding cavity, one side end surface of the closed vertebral lamina, far away from the axle center of the closed cover plate, is positioned on the same side as the side end surface of the closed cavity, far away from the closed cover plate, is fixedly provided with a closed spring between the side end surfaces of the closed cavity, far away from the closed cover plate, the detection box at the upper end of the left side of the working cavity is fixedly provided with a translation motor, the right end surface of the translation motor is dynamically connected with a translation screw rod which penetrates through the left and right wall surfaces of the working cavity, and an extraction body which can move left and right is matched with the outer circular surface of the translation screw rod positioned on the upper side of the cancer cell culture bottle, a suction device storage cavity is arranged in the extractor body, a suction pipe extending hole is communicated between the lower wall surface of the suction device storage cavity and the lower end surface of the extractor body, a supporting table is fixedly arranged on the lower wall surface of the working cavity on the right side of the cancer cell culture bottle, a temperature contrast detection mechanism is arranged inside the supporting table, a liquid medicine detection culture dish is fixedly arranged on the upper end surface of the support platform, the upper end surface of the liquid medicine detection culture dish is communicated with a liquid medicine culture cavity with an upward opening, the liquid medicine to be tested can be stored in the liquid medicine culture cavity, a temperature detection cavity is arranged in the liquid medicine detection culture dish at the lower side of the liquid medicine culture cavity, the temperature detection cavity can sense the temperature in the liquid medicine detection culture dish, a culture bottle detection cavity is arranged in the cancer cell culture bottle on the lower side of the cancer cell culture cavity, and the culture bottle detection cavity can detect the temperature in the cancer cell culture cavity in real time; a translational sliding cavity is arranged in the detection box at the upper side of the working cavity, a translational sliding groove is communicated between the lower wall surface of the translational sliding cavity and the upper wall surface of the working cavity, a guide sliding plate capable of sliding left and right is arranged in the translational sliding cavity at the position corresponding to the extraction machine body, a connecting rod penetrating through the translational sliding groove and extending to the inside of the working cavity is fixedly arranged on the lower end surface of the guide sliding plate, and the lower end of the connecting rod is fixedly connected with the upper end surface of the extraction machine body; a rack lifting cavity is arranged in the extractor body on the left side of the storage cavity of the suction device, a lifting guide chute extending backwards is communicated with the rear wall surface of the rack lifting cavity, a tie rod moving chute is communicated between the right wall surface of the rack lifting cavity and the inner wall surface of the storage cavity of the suction device, a lifting motor is fixedly arranged in the extractor body on the left side corresponding to the middle position of the storage cavity of the suction device, the right end surface of the lifting motor is in power connection with a gear shaft extending into the rack lifting cavity, and a gear is fixedly arranged at the right end of the gear shaft; a rack capable of sliding up and down is arranged on the rear side of the gear, the outer circular surface of the gear is meshed with the front end surface of the rack, a lifting guide sliding rod extending into the lifting guide sliding groove is fixedly arranged on the rear end of the rack, the rear end of the lifting guide sliding rod can slide up and down in the lifting guide sliding groove, a transverse pull rod penetrating through the transverse pull rod moving groove and extending into the storage cavity of the suction device is fixedly arranged on the right end surface of the lower side of the rack, an annular suction tube fixing ring is fixedly arranged on the right end of the transverse pull rod, a vertically-arranged suction tube is fixedly arranged on the inner circular surface of the suction tube fixing ring, suction tube rubber heads communicated with each other are fixedly arranged on the upper end of the suction tube, mutually-symmetrical upper extrusion blocks are fixedly arranged on the front wall surface and the rear wall surface of the storage cavity of the suction device on the upper side of the suction tube rubber heads, and the suction tube rubber heads can be extruded when continuously moving up, the front wall and the rear wall of the storage cavity of the suction device at the lower side of the upper extrusion block are fixedly provided with lower extrusion blocks which are mutually symmetrical, and the rubber head of the suction pipe can be extruded by the lower extrusion blocks when moving to the lower limit; a temperature detection and regulation cavity is arranged in the support platform at the lower side of the temperature detection cavity, the left end and the right end of the temperature detection and regulation cavity are fixedly provided with temperature guide plates which are mutually symmetrical, the heat conducting plate can transfer heat, the inner wall surface of the temperature detection regulation cavity is provided with a detection plate capable of sliding left and right, the temperature detection and control cavities on the left side and the right side of the detection plate are filled with inert gases with the same quality and quantity, a culture bottle detection pipeline is communicated between the lower wall surface of the culture bottle detection cavity and the left end surface of the left temperature conduction plate, a temperature detection conveying pipe is communicated between the lower wall surface of the temperature detection cavity and the right end surface of the temperature guide plate on the right side, an opening and closing cavity is arranged at the lower end of the temperature detection conveying pipe close to one side of the temperature detection regulating cavity, and an opening and closing channel is communicated between the upper wall surface of the opening and closing cavity and the temperature detection conveying pipe; a trigger block extending leftwards is fixedly arranged on the right wall surface of the working cavity at the position corresponding to the lower end of the extractor body, a trigger cavity is arranged in the trigger block, a trigger plate capable of moving left and right is arranged on the inner wall surface of the trigger cavity, a trigger rod extending out of the left end surface of the trigger block is fixedly arranged on the left end surface of the trigger plate, a trigger spring is fixedly arranged between the right end surface of the trigger plate and the right wall surface of the trigger cavity, an opening and closing plate capable of sliding up and down is arranged on the inner wall surface of the opening and closing cavity, an opening and closing lifting rod capable of sliding up and down in the opening and closing channel is fixedly arranged on the upper end surface of the opening and closing plate, the opening and closing lifting rod can seal the temperature detection conveying pipe and insulate the gas in the temperature detection cavity, an opening and closing spring is fixedly arranged between the lower end face of the opening and closing plate and the lower wall face of the opening and closing cavity, and a pull rope is fixedly connected between the lower end face of the opening and closing plate and the right end face of the trigger plate; temperature detects regulation and control chamber lower wall intermediate position intercommunication and is equipped with downwardly extending's release and triggers the chamber, release triggers intracavity internal face and is equipped with the release trigger head that can slide from top to bottom, the fixed release signal piece that is equipped with of chamber lower wall face is triggered in the release, under the release trigger head terminal surface with the release trigger chamber is fixed between the wall face and is equipped with the release signal spring, the release trigger head with can control when the release signal piece contacts elevator motor drive and drive the rack continues upward movement one section distance to be accomplished the extrusion of straw rubber head.
CN202110017157.5A 2021-01-07 2021-01-07 Research and development device for mutant small molecule anticancer drugs Active CN112646720B (en)

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