CN210103977U - Cancer cell culture auxiliary assembly - Google Patents
Cancer cell culture auxiliary assembly Download PDFInfo
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- CN210103977U CN210103977U CN201920908486.7U CN201920908486U CN210103977U CN 210103977 U CN210103977 U CN 210103977U CN 201920908486 U CN201920908486 U CN 201920908486U CN 210103977 U CN210103977 U CN 210103977U
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- shaped plate
- cancer cell
- bottle
- cell culture
- groove
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Abstract
The utility model discloses a cancer cell culture auxiliary device, including the culture flask that the opening is upwards, the bottleneck department of culture flask installs the bottle plug, the bottom side of culture flask inside is equipped with support piece, support piece includes the Y shaped plate, the extending direction of each tip of Y shaped plate all is equipped with the extension rod, the extension rod bottom side is close to the position of Y shaped plate and has seted up first mounting groove, the one end fixed connection of this first mounting groove and elastic sheet, the lower extreme of each tip of Y shaped plate has all seted up the second recess, this second recess and elastic sheet's other end fixed connection, the elastic sheet levels under the natural state, the intermediate position of Y shaped plate upper surface has seted up the circular slot; this cancer cell culture auxiliary assembly has increased agitating unit's cross-sectional area, and the rotational speed of the magnetic stirring arm reduces when driving the culture solution rotation, and the shearing force of magnetic stirring arm diminishes, is difficult for haring cancer cell.
Description
Technical Field
The utility model relates to a cell culture equipment technical field specifically is a cancer cell culture auxiliary assembly.
Background
Among the prior art, suspension culture cancer cell can realize the high density culture of cancer cell, relies on magnetic stirrers to realize the stirring, and suspension culture rotor flask is provided with the stirring of taking magnetism, and magnetic stirrers utilizes the principle that like poles repel each other, opposite poles attract of magnetic field, makes magnetic field promote to place and carry out the circumference operation in the stirring of taking magnetism in the container to reach the mesh of stirring liquid. However, the magnetic stirrer realizes stirring, the stirring cross-sectional area of the magnetic stirrer is small, the rotating speed of the magnetic stirrer required for driving the culture solution to rotate is high, the shearing force of the magnetic stirrer is large, and cancer cells are easily damaged.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to overcome current defect, a cancer cell culture auxiliary assembly is provided, agitating unit's cross-sectional area has been increased, the rotation rate of the rotatory time magnetic stirring of drive culture solution reduces, the shearing force of the magnetic stirring diminishes, be difficult for haring cancer cell, it is little to effectively solve magnetic stirring cross-sectional area in the background art, it is fast to drive the rotation rate that the culture solution is rotatory to need magnetic stirring, magnetic stirring shearing force is great, damage cancer cell's problem easily.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a cancer cell culture auxiliary assembly, includes the ascending blake bottle of opening, and the bottleneck department of blake bottle installs the bottle plug, the inside bottom side of blake bottle is equipped with support piece, and support piece includes the Y shaped plate, and the extending direction of every tip of Y shaped plate all is equipped with the extension rod, and the extension rod bottom side is close to the position of Y shaped plate and has seted up first mounting groove, the one end fixed connection of this first mounting groove and flexure strip, and the second recess has all been seted up to the lower extreme of every tip of Y shaped plate, and the other end fixed connection of this second recess and flexure strip levels under the flexure strip natural state, and the circular slot has been seted up to the intermediate position of.
The lower extreme of circular slot and dwang rotates to be connected, the middle part fixed connection of the upper end of dwang and magnet lower extreme, the upper end of magnet and the lower extreme fixed connection of vertical pole install a plurality of stirring pieces on the vertical pole, and the upper end of vertical pole is located the blake bottle.
As an optimal technical scheme of the utility model, a plurality of flutings have been seted up to the one end that the Y shaped plate was kept away from to the extension rod, and fluting and extension rod length direction are perpendicular.
As a preferred technical scheme of the utility model, the stirring piece includes the elastic rubber pipe, and the elastic rubber pipe box is established on vertical pole, and the diameter of inner chamber is less than the diameter of vertical pole under the elastic rubber pipe natural state, and the outside of elastic rubber pipe is fixed with the blade.
As an optimal technical scheme of the utility model, the blade is vertical dull and stereotyped, and vertical dull and stereotyped width direction is perpendicular with the length direction of vertical pole, and vertical dull and stereotyped width is less than the radius of blake bottle mouth department.
As a preferred technical scheme of the utility model, still include the bolt, the downside fluting has been seted up to the position that Y shaped plate bottom side corresponds the circular slot, and the intercommunicating pore has been seted up to downside fluting top side, intercommunicating pore intercommunication circular slot and downside fluting, the lower extreme of dwang set up threaded hole, the upper end of bolt pass downside fluting, intercommunicating pore and with screw hole threaded connection.
As a preferred technical scheme of the utility model, the bottom side of blake bottle and the top side fixed connection of rubber ring.
As a preferred technical scheme of the utility model, positioning groove has been seted up to the bottom side of bottle plug, and positioning groove is stretched into and is connected with the rotation of positioning groove in the upper end of vertical pole.
As an optimized technical proposal of the utility model, the diameter of the virtual circle of the Y-shaped plate end part is not more than the diameter of the bottle mouth of the culture bottle.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the utility model discloses cancer cell culture auxiliary assembly, magnet pass through vertical pole and drive the stirring piece rotation to reach the purpose of stirring liquid, the effective area of the stirring cross section of blade is big, under the rotatory invariable rotational speed of culture solution in the blake bottle, the rotation rate that needs blade and magnet diminishes, and the speed of shearing force and agitating unit is directly proportional, has reduced the shearing force of blade and magnet, is difficult for damaging the cancer cell.
2. The utility model discloses the cancer cell culture auxiliary assembly of example, the extension rod is rotatory downwards, and the flexure strip is crooked deformation, and support piece gets into the blake bottle in convenient, and the extension rod keeps away from the one end of Y shaped plate and the inner wall contact of blake bottle, has improved the stability of stirring piece and magnet.
3. The utility model discloses the cancer cell culture auxiliary assembly of example breaks off the unnecessary length of extension rod through the fluting with the fingers and thumb according to the difference of the inside diameter of blake bottle, and the extension rod equals the inside diameter of blake bottle to the length of circular slot, and this support piece suitability is strong.
4. The utility model discloses cancer cell culture auxiliary assembly, agitating unit are located the bottle, and the power take off part and the power input part that pass agitating unit are kept apart completely, make the bottle be in totally closed state, stir efficiently to can reduce stirring speed, reduce the damage to the cell.
Drawings
FIG. 1 is a schematic view of the structure of the present invention;
FIG. 2 is a schematic structural view of the stirring member and the supporting member of the present invention;
fig. 3 is an enlarged schematic view of the structure at a position a of the present invention.
In the figure: 1 vertical rod, 2 stirring pieces, 21 elastic rubber tubes, 22 blades, 3 magnets, 4 supporting pieces, 41 grooves, 42 extension rods, 43 elastic pieces, 44 circular grooves, 45Y-shaped plates, 5 rotating rods, 6 bolts, 7 lower side grooves, 8 rubber rings, 9 threaded holes, 10 communicating holes, 11 bottle stoppers and 12 culture bottles.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: the utility model provides a cancer cell culture auxiliary assembly, including ascending blake bottle 12 of opening, bottle plug 11 is installed to the bottleneck department of blake bottle 12, the inside bottom side of blake bottle 12 is equipped with support piece 4, support piece 4 includes Y shaped plate 45, the extending direction of every tip of Y shaped plate 45 all is equipped with extension rod 42, extension rod 42 bottom side has been close to the position of Y shaped plate 45 and has been seted up first mounting groove, the one end fixed connection of this first mounting groove and flexure strip 43, the second recess has all been seted up to the lower extreme of every tip of Y shaped plate 45, the other end fixed connection of this second recess and flexure strip 43, flexure strip 43 levels down under the natural state, circular slot 44 has been seted up to the intermediate position of Y shaped plate 45 upper surface.
The lower extreme of circular slot 44 and dwang 5 rotates to be connected, the middle part fixed connection of the upper end of dwang 5 and the 3 lower extreme of magnet, the upper end of magnet 3 and the lower extreme fixed connection of vertical pole 1 install a plurality of stirring pieces 2 on the vertical pole 1, and the upper end of vertical pole 1 is located blake bottle 12.
The support member 4 conveniently enters the culture bottle 12, and one end of the extension rod 42 far away from the Y-shaped plate 45 is contacted with the inner wall of the culture bottle 12, so that the stability of the stirring member 2 and the magnet 3 is improved; magnet 3 drives stirring piece 2 through vertical pole 1 and rotates to reach the purpose of stirring liquid, the effective area of blade 22's stirring cross section is big, has reduced the rotational speed of blade 22 and magnet 3, has reduced the shearing force of blade 22 and magnet 3, is difficult for haring cancer cell.
A plurality of slots 41 are formed in one end of the extension rod 42, which is far away from the Y-shaped plate 45, and the length directions of the slots 41 and the extension rod 42 are vertical; according to the difference of the inner diameter of the culture bottle 12, the redundant length of the extension rod 42 is broken off through the slot 41, the length from the extension rod 42 to the circular slot 44 is equal to the inner diameter of the culture bottle 12, and the support piece 4 has strong applicability.
The stirring part 2 comprises an elastic rubber tube 21, the elastic rubber tube 21 is sleeved on the vertical rod 1, the diameter of an inner cavity of the elastic rubber tube 21 is smaller than that of the vertical rod 1 in a natural state, blades 22 are fixed on the outer side of the elastic rubber tube 21, the position of the elastic rubber tube 21 on the vertical rod 1 is moved so as to adjust the position of the blades 22 on the vertical rod 1, and the position of the stirring part 2 on the vertical rod 1 is convenient to adjust; the blades 22 are vertical flat plates, the width direction of each vertical flat plate is perpendicular to the length direction of the vertical rod 1, the width of each vertical flat plate is smaller than the radius of the bottle mouth of the culture bottle 12, and the blades 22 can be conveniently taken out of or put into the bottle mouth of the culture bottle 12.
Specifically, as shown in fig. 3, the magnetic flux linkage rotating rod further comprises a bolt 6, a lower side groove 7 is formed in the position, corresponding to the circular groove 44, of the bottom side of the Y-shaped plate 45, a communicating hole 10 is formed in the top side of the lower side groove 7, the communicating hole 10 communicates the circular groove 44 with the lower side groove 7, a threaded hole 9 is formed in the lower end of the rotating rod 5, the lower side groove 7 is passed through the upper end of the bolt 6, the communicating hole 10 is connected with the threaded hole 9 in a threaded mode, the bolt 6 prevents the rotating rod 5 and the circular groove 44 from being separated, the rotating rod 5 drives the bolt 6 to.
The bottom side of blake bottle 12 and the top side fixed connection of rubber ring 8, rubber ring 8 has improved the shock attenuation performance of blake bottle 12, and rubber ring 8 avoids blake bottle 12 direct and outside magnetic stirrers place the platform contact, avoids leading to the heat transfer of blake bottle 12 bottom to place the platform for outside magnetic stirrers because of heat-conduction, causes the temperature reduction of blake bottle 12 culture solution, guarantees the normal culture of cancer cell in the blake bottle 12.
The diameter of the imaginary circle at the end of the Y-shaped plate 45 in this embodiment is not larger than the diameter of the mouth of the culture bottle 12.
When in use:
in the prior art, the diameter of the mouth of the culture bottle 12 is smaller than the diameter of the inside of the culture bottle 12;
the equipment is operated on an ultra-clean sterile table, the bottom end of a vertical rod 1 extends into a circular groove 44, the upper end of a bolt 6 penetrates through a lower side slot 7 and a communicating hole 10 and is in threaded connection with a threaded hole 9, an extension rod 42 rotates downwards, an elastic sheet 43 is bent and deformed, elastic potential energy is accumulated in the elastic sheet 43, a support member 4 enters a culture bottle 12, the extension rod 42 is released, the elastic sheet 43 enables the extension rod 42 to be horizontal, the support member 4 enters the bottom of the culture bottle 12, and one end, far away from a Y-shaped plate 45, of the extension rod 42 is in contact with the inner wall of the culture bottle 12;
the culture solution and the cancer cells are injected into the culture bottle 12, and the bottle stopper 11 blocks the bottle mouth of the culture bottle 12;
this equipment is put placing bench of outside magnetic stirrers, magnetic stirrers utilizes like magnetic poles of magnetic field to repel each other, the principle that opposite poles attracted, make magnetic field promote to place and carry out the circumference operation at 12 magnetic magnet 3 in the blake bottle, magnet 3 drives stirring 2 rotations through vertical pole 1, thereby reach the purpose of stirring liquid, the effective area of blade 22's stirring cross section is big, the rotation rate of blade 22 and magnet 3 has been reduced, the shearing force of blade 22 and magnet 3 has been reduced, be difficult for damaging the cancer cell.
The lower end of the rotating rod 5 is rotatably connected with the Y-shaped plate 45 through the bolt 6, and the vertical rod 1 and the magnet 3 are not prone to toppling;
when the support member 4 is removed, the support member 4 is pulled upwards by the vertical rod 1 to move towards the mouth of the culture bottle 12, the extension rod 42 is bent downwards, and the support member 4 is removed from the culture bottle 12.
Example two: as shown in the figures, the present embodiment discloses a cancer cell culture auxiliary device, the structure of which is substantially the same as that of the first embodiment, except that the present embodiment does not include the bolt 6, the bottom side of the bottle plug 11 is provided with a positioning groove, and the upper end of the vertical rod 1 extends into the positioning groove and is rotatably connected with the positioning groove.
When in use:
the upper end of the vertical rod 1 is positioned by the positioning groove on the bottle stopper 11, the lower end of the rotating rod 5 is positioned by the circular groove 44 on the Y-shaped plate 45, the vertical rod 1 and the magnet 3 are conveniently positioned and are not easy to cut obliquely, and the stirring effect of the blades 22 and the magnet 3 is ensured.
In the prior art, the magnetic stirrer utilizes the principle that like poles of a magnetic field repel each other and opposite poles of the magnetic field attract each other, so that the magnetic stirrer placed in a container is pushed by the magnetic field to perform circumferential operation, the aim of stirring liquid is fulfilled, and the problem that cancer cells are damaged due to the fact that the rotating speed of the magnetic stirrer is too high exists. And the utility model discloses magnet 3 drives stirring 2 rotations through vertical pole 1 to reach the purpose of stirring liquid, the effective area of blade 22's stirring cross section is big, has reduced the rotation rate of blade 22 and magnet 3, has reduced the shearing force of blade 22 and magnet 3, is difficult for damaging the cancer cell. Like pulping, if the blade rotates slowly, e.g., 3r/s, the blade will only push the beans away, but if the blade rotates quickly, the instant force of contact between the blade and the beans is large, and the blade will crush or cut the beans.
The support member 4 is conveniently inserted into the culture bottle 12, and the extension rod 42 is far away from one end of the Y-shaped plate 45 and contacts with the inner wall of the culture bottle 12, so that the stability of the stirring member 2 and the magnet 3 is improved. The integral inclination of the vertical rod 1, the stirring piece 2 and the magnet 3 is prevented from influencing the integral stirring effect.
According to the difference of the inner diameter of the culture bottle 12, the redundant length of the extension rod 42 is broken off through the slot 41, the length from the extension rod 42 to the circular slot 44 is equal to the inner diameter of the culture bottle 12, and the support piece 4 has strong applicability.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a cancer cell culture auxiliary assembly, includes ascending blake bottle (12) of opening, and bottle plug (11), its characterized in that are installed to the bottleneck department of blake bottle (12): the bottom side in the culture bottle (12) is provided with a support piece (4), the support piece (4) comprises a Y-shaped plate (45), an extension rod (42) is arranged in the extension direction of each end part of the Y-shaped plate (45), a first mounting groove is formed in the position, close to the Y-shaped plate (45), of the bottom side of the extension rod (42), the first mounting groove is fixedly connected with one end of an elastic sheet (43), a second groove is formed in the lower end of each end part of the Y-shaped plate (45), the second groove is fixedly connected with the other end of the elastic sheet (43), the elastic sheet (43) is flat in a natural state, and a circular groove (44) is formed in the middle position of the upper surface of the Y-shaped plate;
the lower extreme of circular slot (44) and dwang (5) rotates to be connected, the middle part fixed connection of the upper end of dwang (5) and magnet (3) lower extreme, the upper end of magnet (3) and the lower extreme fixed connection of vertical pole (1), install a plurality of stirring pieces (2) on vertical pole (1), the upper end of vertical pole (1) is located blake bottle (12).
2. The cancer cell culture assisting apparatus according to claim 1, wherein: a plurality of slots (41) are formed in one end, far away from the Y-shaped plate (45), of the extension rod (42), and the length directions of the slots (41) and the extension rod (42) are perpendicular.
3. The cancer cell culture assisting apparatus according to claim 1, wherein: stirring piece (2) are including elasticity rubber tube (21), and elasticity rubber tube (21) cover is established on vertical pole (1), and the diameter of inner chamber is less than the diameter of vertical pole (1) under elasticity rubber tube (21) the natural state, and the outside of elasticity rubber tube (21) is fixed with blade (22).
4. The cancer cell culture assisting apparatus according to claim 3, wherein: the blades (22) are vertical flat plates, the width direction of each vertical flat plate is perpendicular to the length direction of the vertical rod (1), and the width of each vertical flat plate is smaller than the radius of the bottleneck of the culture bottle (12).
5. The cancer cell culture assisting apparatus according to claim 1, wherein: the novel rotary rod is characterized by further comprising a bolt (6), the lower side groove (7) is formed in the position, corresponding to the circular groove (44), of the bottom side of the Y-shaped plate (45), the communicating hole (10) is formed in the top side of the lower side groove (7), the communicating hole (10) is communicated with the circular groove (44) and the lower side groove (7), the threaded hole (9) is formed in the lower end of the rotary rod (5), and the upper end of the bolt (6) penetrates through the lower side groove (7) and the communicating hole (10) and is in threaded connection.
6. The cancer cell culture assisting apparatus according to claim 1, wherein: the bottom side of the culture bottle (12) is fixedly connected with the top side of the rubber ring (8).
7. The cancer cell culture assisting apparatus according to claim 1, wherein: the bottom side of the bottle stopper (11) is provided with a positioning groove, and the upper end of the vertical rod (1) extends into the positioning groove and is rotatably connected with the positioning groove.
8. The cancer cell culture assisting apparatus according to claim 1, wherein: the diameter of a virtual circle where the end part of the Y-shaped plate (45) is positioned is not more than that of the bottleneck of the culture bottle (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920908486.7U CN210103977U (en) | 2019-06-17 | 2019-06-17 | Cancer cell culture auxiliary assembly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920908486.7U CN210103977U (en) | 2019-06-17 | 2019-06-17 | Cancer cell culture auxiliary assembly |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210103977U true CN210103977U (en) | 2020-02-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920908486.7U Expired - Fee Related CN210103977U (en) | 2019-06-17 | 2019-06-17 | Cancer cell culture auxiliary assembly |
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| Country | Link |
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| CN (1) | CN210103977U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646720A (en) * | 2021-01-07 | 2021-04-13 | 广州市言康生物科技有限公司 | Research and development device for mutant small molecule anticancer drugs |
-
2019
- 2019-06-17 CN CN201920908486.7U patent/CN210103977U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646720A (en) * | 2021-01-07 | 2021-04-13 | 广州市言康生物科技有限公司 | Research and development device for mutant small molecule anticancer drugs |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200221 Termination date: 20210617 |
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| CF01 | Termination of patent right due to non-payment of annual fee |