CN210103977U - A kind of auxiliary equipment for cancer cell culture - Google Patents

Abstract

The utility model discloses a cancer cell culture auxiliary device, including the culture flask that the opening is upwards, the bottleneck department of culture flask installs the bottle plug, the bottom side of culture flask inside is equipped with support piece, support piece includes the Y shaped plate, the extending direction of each tip of Y shaped plate all is equipped with the extension rod, the extension rod bottom side is close to the position of Y shaped plate and has seted up first mounting groove, the one end fixed connection of this first mounting groove and elastic sheet, the lower extreme of each tip of Y shaped plate has all seted up the second recess, this second recess and elastic sheet's other end fixed connection, the elastic sheet levels under the natural state, the intermediate position of Y shaped plate upper surface has seted up the circular slot; this cancer cell culture auxiliary assembly has increased agitating unit's cross-sectional area, and the rotational speed of the magnetic stirring arm reduces when driving the culture solution rotation, and the shearing force of magnetic stirring arm diminishes, is difficult for haring cancer cell.

Description

A kind of auxiliary equipment for cancer cell culture

technical field

The utility model relates to the technical field of cell culturing equipment, in particular to an auxiliary equipment for culturing cancer cells.

Background technique

In the prior art, the suspension culture of cancer cells can achieve high-density culture of cancer cells, and the stirring is realized by means of a magnetic stirrer. The suspension culture spinner flask is provided with a magnetic stirrer, and the magnetic stirrer utilizes the magnetic field of the same sex to repel and the opposite sex to attract. The principle of the magnetic field pushes the magnetic stirring bar placed in the container to rotate in a circle, so as to achieve the purpose of stirring the liquid. However, the magnetic stirrer realizes the stirring. The magnetic stirrer has a small cross-sectional area for stirring, and the rotation of the magnetic stirrer requires a fast rotation speed. The magnetic stirrer has a large shear force, which is easy to damage the cancer cells.

Utility model content

The technical problem to be solved by the utility model is to overcome the existing defects and provide an auxiliary device for culturing cancer cells, which increases the cross-sectional area of the stirring device, reduces the rotation speed of the magnetic stirring bar when driving the culture solution to rotate, and the magnetic The shear force of the stirring bar becomes smaller, which is not easy to damage the cancer cells, which can effectively solve the problem that the magnetic stirring bar in the background technology has a small stirring cross-sectional area, and the rotation speed of the magnetic stirring bar is required to drive the culture medium to rotate. Larger and easier to damage cancer cells.

In order to achieve the above purpose, the present invention provides the following technical solutions: an auxiliary device for culturing cancer cells, comprising a culture bottle with an upward opening, a bottle stopper is installed at the bottle mouth of the culture bottle, and a support is provided on the bottom side of the inside of the culture bottle. The support member includes a Y-shaped plate, and each end of the Y-shaped plate is provided with an extension rod in the extension direction, and a first installation groove is opened on the bottom of the extension rod near the Y-shaped plate. One end of the Y-shaped plate is fixedly connected, the lower end of each end of the Y-shaped plate is provided with a second groove, the second groove is fixedly connected with the other end of the elastic sheet, the elastic sheet is flat in its natural state, and the middle of the upper surface of the Y-shaped plate is There is a circular groove in the position.

The circular groove is rotatably connected with the lower end of the rotating rod, the upper end of the rotating rod is fixedly connected with the middle of the lower end of the magnet, and the upper end of the magnet is fixedly connected with the lower end of the vertical rod. inside the culture bottle.

As a preferred technical solution of the present invention, the end of the extension rod away from the Y-shaped plate is provided with a plurality of slots, and the slot is perpendicular to the length direction of the extension rod.

As a preferred technical solution of the present invention, the stirring member includes an elastic rubber tube, the elastic rubber tube is sleeved on the vertical rod, and the diameter of the inner cavity of the elastic rubber tube in the natural state is smaller than the diameter of the vertical rod, and the elastic rubber tube is smaller than the diameter of the vertical rod. The outer side of the rubber tube is fixed with a blade.

As a preferred technical solution of the present invention, the blade is a vertical plate, the width direction of the vertical plate is perpendicular to the length direction of the vertical rod, and the width of the vertical plate is smaller than the radius of the bottle mouth of the culture bottle.

As a preferred technical solution of the present invention, it also includes bolts, a lower side slot is formed on the bottom side of the Y-shaped plate at a position corresponding to the circular groove, a communication hole is formed on the top side of the lower side slot, and the communication hole communicates with the circular groove The lower end of the rotating rod is provided with a threaded hole, and the upper end of the bolt passes through the lower slot and the communication hole and is threadedly connected with the threaded hole.

As a preferred technical solution of the present invention, the bottom side of the culture flask and the top side of the rubber ring are fixedly connected.

As a preferred technical solution of the present invention, the bottom side of the bottle stopper is provided with a positioning groove, and the upper end of the vertical rod extends into the positioning groove and is rotatably connected with the positioning groove.

As a preferred technical solution of the present invention, the diameter of the virtual circle where the end of the Y-shaped plate is located is not greater than the diameter of the mouth of the culture bottle.

Compared with the prior art, the beneficial effects of the present utility model are:

1. In the auxiliary equipment for culturing cancer cells in the example of the present invention, the magnet drives the stirring member to rotate through the vertical rod, so as to achieve the purpose of stirring the liquid, the effective area of the stirring cross section of the blade is large, and the culture liquid in the culture bottle rotates at a constant speed , The rotation speed of the blade and the magnet needs to be reduced, and the shear force is proportional to the speed of the stirring device, which reduces the shear force of the blade and the magnet, and is not easy to damage the cancer cells.

2. In the auxiliary equipment for culturing cancer cells in the example of the present invention, the extension rod rotates downward, the elastic sheet is bent and deformed, the support member is convenient to enter the culture bottle, and the end of the extension rod away from the Y-shaped plate contacts the inner wall of the culture bottle, which improves the performance of the culture bottle. Stability of stirring elements and magnets.

3. The auxiliary equipment for culturing cancer cells in the example of the present invention, according to the inner diameter of the culture bottle, removes the excess length of the extension rod by slotting, and the length of the extension rod to the circular groove is equal to the inner diameter of the culture bottle. This support is suitable for Strong sex.

4. In the auxiliary equipment for culturing cancer cells in the example of the present invention, the stirring device is located in the bottle body, and the power output part and the power input part of the transmission stirring device are completely isolated, so that the bottle body is in a fully closed state, and the stirring efficiency is high, thereby reducing the energy consumption. Stirring speed to reduce damage to cells.

Description of drawings

Fig. 1 is the front view structure schematic diagram of the utility model;

Fig. 2 is the structural schematic diagram of the stirring member and the supporting member of the present invention;

FIG. 3 is an enlarged schematic view of the structure at A of the present invention.

In the picture: 1 vertical rod, 2 stirring piece, 21 elastic rubber tube, 22 blade, 3 magnet, 4 support piece, 41 slot, 42 extension rod, 43 elastic sheet, 44 round groove, 45 Y-shaped plate, 5 rotation Rod, 6 bolts, 7 grooves on the lower side, 8 rubber rings, 9 threaded holes, 10 communication holes, 11 bottle stoppers, 12 culture flasks.

Detailed ways

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the implementations. example. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

1-3, the present invention provides a technical solution: an auxiliary device for culturing cancer cells, comprising a culture bottle 12 with an upward opening, a bottle stopper 11 is installed at the bottle mouth of the culture bottle 12, and a bottle inside the culture bottle 12 is The bottom side is provided with a support member 4, and the support member 4 includes a Y-shaped plate 45. An extension rod 42 is provided in the extending direction of each end of the Y-shaped plate 45. An installation groove, the first installation groove is fixedly connected with one end of the elastic sheet 43, the lower end of each end of the Y-shaped plate 45 is provided with a second groove, and the second groove is fixedly connected with the other end of the elastic sheet 43 , the elastic sheet 43 is flat in a natural state, and a circular groove 44 is opened in the middle position of the upper surface of the Y-shaped plate 45 .

The circular groove 44 is rotatably connected with the lower end of the rotating rod 5, the upper end of the rotating rod 5 is fixedly connected with the middle of the lower end of the magnet 3, the upper end of the magnet 3 is fixedly connected with the lower end of the vertical rod 1, and a number of stirring pieces are installed on the vertical rod 1 2. The upper end of the vertical rod 1 is located in the culture flask 12 .

It is convenient for the support member 4 to enter the culture flask 12, and the end of the extension rod 42 away from the Y-shaped plate 45 is in contact with the inner wall of the culture flask 12, which improves the stability of the stirring member 2 and the magnet 3; the magnet 3 drives the stirring through the vertical rod 1 The element 2 rotates to achieve the purpose of stirring the liquid. The effective area of the stirring cross section of the blade 22 is large, which reduces the rotation speed of the blade 22 and the magnet 3, reduces the shear force of the blade 22 and the magnet 3, and is not easy to damage cancer cells.

The end of the extension rod 42 away from the Y-shaped plate 45 is provided with a number of slots 41, and the slot 41 and the extension rod 42 are perpendicular to the length direction; The length from the extension rod 42 to the circular groove 44 is equal to the inner diameter of the culture flask 12 , and the support 4 has strong applicability.

The stirring member 2 includes an elastic rubber tube 21, which is sleeved on the vertical rod 1, and the diameter of the inner cavity of the elastic rubber tube 21 is smaller than the diameter of the vertical rod 1 in a natural state, and the outer side of the elastic rubber tube 21 is fixed with a The blade 22 moves the position of the elastic rubber tube 21 on the vertical rod 1 to adjust the position of the blade 22 on the vertical rod 1, and the position of the stirring element 2 on the vertical rod 1 is convenient to adjust; the blade 22 is vertical Plate, the width direction of the vertical plate is perpendicular to the length direction of the vertical rod 1, and the width of the vertical plate is smaller than the radius of the bottle mouth of the culture bottle 12, the blade 22 can be easily taken out from the bottle mouth of the culture bottle 12 or put into a.

Specifically, as shown in FIG. 3 , a bolt 6 is also included, a lower side slot 7 is formed on the bottom side of the Y-shaped plate 45 corresponding to the circular groove 44 , and a communication hole 10 is formed on the top side of the lower side slot 7 , and the communication hole 10 Connect the circular groove 44 and the lower side slot 7, the lower end of the rotating rod 5 is provided with a threaded hole 9, the upper end of the bolt 6 passes through the lower side slot 7, the communication hole 10 and is threadedly connected with the threaded hole 9, the bolt 6 prevents the rotation of the rod 5 and the circular groove 44 are disengaged, and when the rotating rod 5 rotates, the rotating rod 5 drives the bolt 6 to rotate synchronously, and the bolt 6 does not affect the magnet 3 to drive the rotating rod 5.

The bottom side of the culture flask 12 is fixedly connected with the top side of the rubber ring 8. The rubber ring 8 improves the shock absorption performance of the culture flask 12, and the rubber ring 8 prevents the culture flask 12 from directly contacting the placing table of the external magnetic stirrer, thereby preventing the The heat conduction causes the heat at the bottom of the culture flask 12 to be transferred to the placing table of the external magnetic stirrer, which causes the temperature of the culture liquid in the culture flask 12 to decrease, and ensures the normal culture of cancer cells in the culture flask 12 .

In this embodiment, the diameter of the virtual circle where the end of the Y-shaped plate 45 is located is not larger than the diameter of the mouth of the culture bottle 12 .

When using:

In the prior art, the diameter at the mouth of the culture bottle 12 is smaller than the diameter inside the culture bottle 12;

When the equipment is operated on the ultra-clean aseptic table, the bottom end of the vertical rod 1 extends into the circular groove 44, the upper end of the bolt 6 passes through the lower side slot 7, the communication hole 10 and is threadedly connected with the threaded hole 9, extending The rod 42 is rotated downward, the elastic sheet 43 is bent and deformed, and elastic potential energy is accumulated in the elastic sheet 43, the support 4 enters the culture bottle 12, and the extension rod 42 is released, the elastic sheet 43 makes the extension rod 42 horizontal, and the support 4. Enter the bottom of the culture bottle 12, and the end of the extension rod 42 away from the Y-shaped plate 45 contacts the inner wall of the culture bottle 12;

Injecting culture medium and adding cancer cells into the culture flask 12, the bottle stopper 11 blocks the bottle mouth of the culture flask 12;

This equipment is placed on the placing table of the external magnetic stirrer. The magnetic stirrer uses the principle of the magnetic field to repel each other and attract each other, so that the magnetic field pushes the magnetic magnet 3 placed in the culture flask 12 to rotate in a circle, and the magnet 3 passes through The vertical rod 1 drives the stirring member 2 to rotate, so as to achieve the purpose of stirring the liquid. The effective area of the stirring cross section of the blade 22 is large, which reduces the rotation speed of the blade 22 and the magnet 3, and reduces the shear force of the blade 22 and the magnet 3. , not easy to damage cancer cells.

The bolt 6 connects the lower end of the rotating rod 5 and the Y-shaped plate 45 in rotation, and the vertical rod 1 and the magnet 3 are not easy to fall;

When the support member 4 is removed, the support member 4 is pulled upward by the vertical rod 1 to move toward the bottle mouth of the culture bottle 12 , the extension rod 42 is bent downward, and the support member 4 is removed from the culture bottle 12 .

Embodiment 2: As shown in the figure, this embodiment discloses an auxiliary device for culturing cancer cells. The bottom side of the vertical rod 1 is provided with a positioning groove, and the upper end of the vertical rod 1 extends into the positioning groove and is rotatably connected with the positioning groove.

When using:

The positioning groove on the bottle stopper 11 locates the upper end of the vertical rod 1, the circular groove 44 on the Y-shaped plate 45 locates the lower end of the rotating rod 5, the vertical rod 1 and the magnet 3 are conveniently positioned, and are not easy to be cut obliquely. The stirring effect of magnet 3.

In the prior art, the magnetic stirrer is a magnetic stirrer that uses the principle of the same-sex repulsion and opposite-sex attraction of the magnetic field, so that the magnetic field pushes the magnetic stirrer placed in the container to rotate in a circle, so as to achieve the purpose of stirring the liquid. The problem that the speed of the stirring bar is too fast to damage the cancer cells. The magnet 3 of the present invention drives the stirring member 2 to rotate through the vertical rod 1, so as to achieve the purpose of stirring the liquid, the effective area of the stirring cross section of the blade 22 is large, the rotation speed of the blade 22 and the magnet 3 is reduced, and the blade 22 is reduced. And the shearing force of the magnet 3, it is not easy to damage the cancer cells. Just like making soy milk, if the blade speed is slow, such as 3r/s, the blade will only push the beans away, but if the blade is fast, the momentary force between the blade and the beans will be large, and the blade will smash or cut the beans.

The support member 4 is easy to enter into the culture flask 12 , and the end of the extension rod 42 away from the Y-shaped plate 45 is in contact with the inner wall of the culture flask 12 , which improves the stability of the stirring member 2 and the magnet 3 . Prevent the whole composed of the vertical rod 1, the stirring member 2 and the magnet 3 from tilting, which will affect the overall stirring effect.

Depending on the inner diameter of the culture flask 12, the excess length of the extension rod 42 is removed through the slot 41, and the length from the extension rod 42 to the circular groove 44 is equal to the inner diameter of the culture flask 12. The support 4 has strong applicability.

Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes and modifications can be made to these embodiments without departing from the principles and spirit of the present invention , alternatives and modifications, the scope of the present invention is defined by the appended claims and their equivalents.

Claims (8)

1. An auxiliary device for culturing cancer cells, comprising a culture bottle (12) with an upward opening, a bottle stopper (11) is installed at the bottle mouth of the culture bottle (12), and characterized in that: the inside of the culture bottle (12) The bottom side is provided with a support member (4), the support member (4) includes a Y-shaped plate (45), and an extension rod (42) is provided in the extending direction of each end of the Y-shaped plate (45). The extension rod (42) A first installation groove is opened at the position of the bottom side close to the Y-shaped plate (45), the first installation groove is fixedly connected with one end of the elastic sheet (43), and the lower end of each end of the Y-shaped plate (45) is provided with a first installation groove. Two grooves, the second groove is fixedly connected to the other end of the elastic sheet (43), the elastic sheet (43) is flat in its natural state, and a circular groove (44) is formed in the middle of the upper surface of the Y-shaped plate (45); The circular groove (44) is rotatably connected with the lower end of the rotating rod (5), the upper end of the rotating rod (5) is fixedly connected with the middle of the lower end of the magnet (3), and the upper end of the magnet (3) is fixed with the lower end of the vertical rod (1). connected, a plurality of stirring elements (2) are installed on the vertical rod (1), and the upper end of the vertical rod (1) is located in the culture flask (12). 2. The cancer cell culture auxiliary device according to claim 1, characterized in that: one end of the extension rod (42) away from the Y-shaped plate (45) is provided with a plurality of slots (41), the slots (41) and The length direction of the extension rod (42) is vertical. 3 . The auxiliary device for culturing cancer cells according to claim 1 , wherein the stirring member ( 2 ) comprises an elastic rubber tube ( 21 ), and the elastic rubber tube ( 21 ) is sleeved on the vertical rod ( 1 ). 4 . , and the diameter of the inner cavity of the elastic rubber tube (21) in the natural state is smaller than the diameter of the vertical rod (1), and the outer side of the elastic rubber tube (21) is fixed with a blade (22). 4 . The auxiliary device for culturing cancer cells according to claim 3 , wherein the blade ( 22 ) is a vertical plate, the width direction of the vertical plate and the length direction of the vertical rod ( 1 ) are perpendicular, and the vertical direction is vertical. 5 . The width of the straight plate is smaller than the radius at the mouth of the culture flask (12). 5 . The auxiliary device for culturing cancer cells according to claim 1 , further comprising a bolt ( 6 ), and a lower side slot is provided on the bottom side of the Y-shaped plate ( 45 ) corresponding to the position of the circular groove ( 44 ). 6 . (7), the lower side slot (7) is provided with a communicating hole (10) on the top side, the communicating hole (10) communicates with the circular groove (44) and the lower side slot (7), and the lower end of the rotating rod (5) is provided with a In the threaded hole (9), the upper end of the bolt (6) passes through the lower side slot (7), the communication hole (10) and is threadedly connected with the threaded hole (9). 6 . The auxiliary device for culturing cancer cells according to claim 1 , wherein the bottom side of the culture flask ( 12 ) is fixedly connected with the top side of the rubber ring ( 8 ). 7 . 7 . The cancer cell culture auxiliary device according to claim 1 , wherein a positioning groove is formed on the bottom side of the bottle stopper ( 11 ), and the upper end of the vertical rod ( 1 ) extends into the positioning groove and is connected with the positioning groove. 8 . Locating grooves rotate the connection. 8 . The auxiliary device for culturing cancer cells according to claim 1 , characterized in that: the diameter of the virtual circle where the end of the Y-shaped plate ( 45 ) is located is not larger than the diameter of the mouth of the culture bottle ( 12 ).

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