CN112641812A - Fermented cordyceps composition with immunity enhancing effect and preparation method and application thereof - Google Patents
Fermented cordyceps composition with immunity enhancing effect and preparation method and application thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/21—Removal of unwanted matter, e.g. deodorisation or detoxification by heating without chemical treatment, e.g. steam treatment, cooking
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a fermented cordyceps composition with an immunity enhancing effect and a preparation method and application thereof, wherein the fermented cordyceps composition comprises the following components in parts by weight: 75-100 parts of paecilomyces hepiali mycelium powder and 0-30 parts of cordyceps militaris; the preparation method comprises the steps of stirring for moistening, moist heat sterilization, drying, crushing, sieving, mixing, granulating and filling. The fermented cordyceps composition provided by the invention combines paecilomyces hepiali mycelium powder with cordyceps militaris, can replace cordyceps sinensis to take, has the effect of enhancing immunity similar to that of the cordyceps sinensis, and fills the gap that no related food or medicine replaces the cordyceps sinensis in the current market.
Description
Technical Field
The invention relates to the technical field of health-care functional foods, in particular to a fermented cordyceps composition with an immunity enhancing effect and a preparation method and application thereof.
Background
Over 2000 years ago, humans were found to have had a disease in epidemic and had resistance to reinfection with the disease, referred to as "immunity". Modern immunology recognizes that immunity is the body's own defense mechanism, and is the body's ability to recognize and destroy any foreign body invaded from the outside, such as viruses, bacteria, etc., to treat aged, damaged, dead, denatured self-cells, and to recognize and treat mutant cells and virus-infected cells in the body. The body with low immunity is very susceptible to infection of bacteria, viruses, fungi and the like, and the most direct manifestation is that the body is easy to get ill. At present, human needs to have the defense capability against various viruses and bacteria, create a healthy body and most importantly enhance the natural immunity and the natural cure capability for various diseases. Therefore, the market potential of the food taking immunity enhancement as a main function is huge.
The cordyceps sinensis is one of three treasures which are all called as wild ginseng and pilose antler in the medical treasury of China, and enjoys extremely high reputation. Cordyceps sinensis is a high-grade rare nourishing medicinal material, is sweet in taste and neutral in nature, has the effects of tonifying kidney and lung, stopping bleeding and reducing phlegm, and is commonly used for treating kidney deficiency and essence deficiency, chronic cough and asthma and the like. Because of its mild nature, it can tonify yin and yang, and is a good medicine for people with weak health and low immunity. However, the growth conditions of the cordyceps sinensis are high, and the cordyceps sinensis is currently endangered to be extinct and is classified as a wild plant (II grade) which is mainly protected by China. Therefore, the search for suitable substitute of Cordyceps is urgently needed.
Paecilomyces hepiali Chen et Dai (sp. nov) is a ubiquitous anamorph of Cordyceps sinensis (Ophiocordyceps sinensis), has main components similar to those of Cordyceps sinensis, is rich in adenosine, cordycepin, cordycepic acid, polysaccharide and other active components, and has effects of regulating immunity, relieving physical fatigue, resisting tumor, etc. The paecilomyces hepiali mycelium can stimulate the proliferation and transformation of splenic lymphocytes of mice, increase the number of antibody-producing cells and the level of serum hemolysin of the mice, promote the carbon clearance of mononuclear-macrophage of the mice and the like (P is less than 0.05), and has the function of enhancing the immunity. The paecilomyces hepiali mycelium can obviously improve the delayed hypersensitivity (P is less than 0.05) of mice, and has up-regulation effect (P is less than 0.05) on the levels of inflammation related factors such as IL-10, IL-12R, INF-alpha and the like, thereby proving that the paecilomyces hepiali has good effect of enhancing the immunity.
Cordyceps militaris (Cordyceps militaris) is also called as Cordyceps militaris, Cordyceps militaris or Cordyceps sinensis, contains various active ingredients such as cordycepin, Cordyceps polysaccharide, cordycepic acid, etc., has effects of regulating immunity, resisting oxidation, resisting tumor, resisting inflammation, etc., and has main chemical components with pharmacological and medicinal effects similar to those of Cordyceps sinensis. The cordyceps militaris sporocarp can obviously promote the natural proliferation effect (P is less than 0.05) of mouse lymphocytes, and simultaneously increase the serum hemolysin level and can enhance the DNFB-induced mouse delayed-type allergic reaction. The Cordyceps militaris extract can obviously increase the white blood cell number and the antibody volume number of a mouse and a model control group (P is less than 0.01), and the Cordyceps militaris has the function of enhancing immunity.
Therefore, the paecilomyces hepiali and the cordyceps militaris are used for replacing cordyceps sinensis, so that the feasibility is high, and no product or related research for developing the product with the efficacy of enhancing the immunity by compounding the paecilomyces hepiali and the cordyceps militaris is found at present. Therefore, the method has wide prospect for developing the food with the immunity enhancing effect by taking the paecilomyces hepiali and the cordyceps militaris as main functional ingredients to replace the cordyceps sinensis.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a fermented cordyceps composition with an immunity enhancing effect for replacing cordyceps sinensis, and a preparation method and application thereof.
In order to achieve the purpose, the invention provides the following technical scheme:
a fermented cordyceps composition with an immunity enhancing effect comprises the following components in parts by weight: 75-100 parts of paecilomyces hepiali mycelium powder and 0-30 parts of cordyceps militaris.
As a preferable technical scheme, the paint comprises the following components in parts by weight: 75-90 parts of paecilomyces hepiali mycelium powder and 10-25 parts of cordyceps militaris powder.
The second aspect of the invention provides a preparation method of the fermented cordyceps composition with the function of enhancing immunity, which comprises the following steps:
(1) stirring for moistening, moist heat sterilization and drying: mixing cordyceps militaris fruit bodies according to a material-liquid ratio of 1: (0.1-0.3) adding water, stirring for moistening for 5-20 min, performing damp-heat sterilization at 121 ℃ for 5-25 min, and drying the moistened cordyceps militaris sporocarp until the water content is less than or equal to 6.5% and the drying temperature is 55-80 ℃;
(2) crushing and sieving: crushing the sterilized and dried cordyceps militaris sporocarp and the paecilomyces hepiali mycelium, and sieving the crushed cordyceps militaris sporocarp and paecilomyces hepiali mycelium by a sieve of 40-200 meshes for later use;
(3) mixing: uniformly mixing the sieved cordyceps militaris sporocarp powder and the sieved paecilomyces hepiali mycelium powder for 10-50 min to obtain a mixture;
(4) and (3) granulating: granulating a mixture of cordyceps militaris sporocarp powder and paecilomyces hepiali mycelium powder to obtain granules;
(5) filling: and filling the particles into a solid preparation to obtain the fermented cordyceps composition.
As a preferable technical scheme, in the step (1), the ratio of cordyceps militaris fruit body to liquid is 1: (0.1-0.2) adding water, stirring for wetting, stirring for 8-15 min, performing damp-heat sterilization at 121 ℃ for 10-20 min, and drying the wetted cordyceps militaris sporocarp until the moisture content is less than or equal to 6.5% and the drying temperature is 60-70 ℃.
As a preferred technical scheme, in the step (2), the sterilized and dried cordyceps militaris sporocarp and the paecilomyces hepiali mycelia are crushed and sieved by a sieve of 80-200 meshes for later use.
As a preferable technical scheme, in the step (3), the mixing time is 10-30 min.
As a preferable technical scheme, in the step (4), the mixture of the cordyceps militaris sporocarp powder and the paecilomyces hepiali mycelium powder is added into a dry-process granulator, and granulation is carried out under the pressure of 1-3 MPa to obtain granules.
Compared with direct sterilization of dry cordyceps militaris, the method has the advantages that the dry cordyceps militaris is soaked and then sterilized, so that the thermal penetration effect of water vapor can be better exerted, the purpose of killing microorganisms is achieved, the dry cordyceps militaris is directly sterilized by a wet-heat method, so that the water vapor cannot sufficiently penetrate through materials, and the sterilization effect is better.
As a preferable technical scheme, in the step (5), the granules are filled into a capsule preparation, and the loading amount is 330 mg/granule, so that the fermented cordyceps composition is obtained.
The third aspect of the invention is to provide the application of the fermented cordyceps composition with the immunity enhancing effect in preparing food or medicine with the immunity enhancing effect.
As a preferable technical scheme, the food comprises functional food and health food.
As a preferred technical solution, the product form of the food or the medicine includes, but is not limited to, powder, capsule, tablet, etc.
Compared with the prior art, the invention has the following advantages:
1. the fermented cordyceps composition provided by the invention has the effect of improving the immunity, the paecilomyces hepiali mycelium powder and the cordyceps militaris are reasonably and scientifically mixed, and animal experiments prove that the composition has the function of enhancing the immunity similar to the effect of the cordyceps sinensis, can be taken by replacing the cordyceps sinensis and fills the blank of the current market;
2. paecilomyces hepiali can be fermented and cultured, is a substitute of natural cordyceps sinensis in pharmaceutical industry and food industry, and the industrial culture technology of cordyceps militaris is mature; the fermented cordyceps composition provided by the invention breaks through the limitation of natural cordyceps raw materials and can be supplied in large quantities;
3. paecilomyces hepiali belongs to 'list of fungus strains for health food', and Cordyceps militaris can be used as new resource food and raw material of health food; the fermented cordyceps composition provided by the invention is a pure natural health-care product, is non-toxic and harmless, and has no side effect;
4. the preparation process of the invention does not add other auxiliary materials, and the preparation method is simple to operate and can be applied to large-scale production.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. The reagents, equipment and methods adopted by the invention are all reagents, equipment and methods which are conventionally and commercially available in the technical field and are conventionally used in the technical field.
Example 1:
a fermented cordyceps composition with an immunity enhancing effect comprises the following components in parts by weight: 85 parts of paecilomyces hepiali mycelium powder and 15 parts of cordyceps militaris powder.
The preparation method comprises the following steps:
(1) stirring for moistening, moist heat sterilization and drying: and (3) mixing the qualified cordyceps militaris sporocarp with the raw materials in a liquid-to-solid ratio of 1: (0.1-0.3) adding water, stirring for 5-20 min for moistening, performing damp-heat sterilization at 121 ℃ for 5-25 min, and drying the moistened cordyceps militaris sporocarp until the moisture content is less than or equal to 6.5% and the drying temperature is 55-80 ℃;
(2) crushing and sieving: crushing the sterilized and dried cordyceps militaris sporocarp and the paecilomyces hepiali mycelium, and sieving the crushed cordyceps militaris sporocarp and paecilomyces hepiali mycelium by a sieve of 40-200 meshes for later use;
(3) mixing: putting the sieved cordyceps militaris sporocarp powder and the sieved paecilomyces hepiali mycelium powder into a V-shaped high-speed mixer, and uniformly mixing for 10-50 min to obtain a mixture;
(4) and (3) granulating: adding a mixture of cordyceps militaris sporocarp powder and paecilomyces hepiali mycelium powder into a feed inlet of a dry-process granulator, granulating under the pressure of 1-3 MPa to obtain granules, and sieving the granules with a 40-mesh sieve;
(5) filling: and filling the particles into a capsule preparation by adopting a full-automatic hard capsule filling machine, wherein the filling amount is 330 mg/particle, so as to obtain the fermented cordyceps composition.
Example 2:
a fermented cordyceps composition with an immunity enhancing effect comprises the following components in parts by weight: 83.3 parts of paecilomyces hepiali mycelium powder and 16.7 parts of cordyceps militaris powder.
The preparation method is the same as that of example 1 and is not repeated herein.
Example 3:
a fermented cordyceps composition with an immunity enhancing effect comprises the following components in parts by weight: 70 parts of paecilomyces hepiali mycelium powder and 30 parts of cordyceps militaris powder.
The preparation method is the same as that of example 1 and is not repeated herein.
Example 4:
a fermented cordyceps composition with an immunity enhancing effect comprises the following components in parts by weight: 95 parts of paecilomyces hepiali mycelium powder and 5 parts of cordyceps militaris powder.
The preparation method is the same as that of example 1 and is not repeated herein.
Effect example 1:
the fermented cordyceps composition prepared in example 2 is used for verifying the function of enhancing immunity
The fermented cordyceps composition prepared in example 2 was orally administered to kunming mice for 4 weeks at 0.17, 0.33, and 099g/kg · BW, respectively, per day, which were 5, 10, and 30 times the recommended daily dose. The specific test substance treatment and administration modes are as follows:
in the experiment, 0.34 g, 0.66 g and 1.98g of samples are weighed, 20ml of sample liquid is prepared by purified water respectively and is used for the intragastric administration of animals with low, medium and high doses respectively, the animals are intragastric administered with the test substance according to the dose of 0.1ml/10g & BW every day, and the negative control group is intragastric administered with 0.1ml/10g & BW purified water every day.
The results show that: as shown in table 1, the sheep red blood cell induced delayed allergy test of the mice is positive, which indicates that the test sample has the function of enhancing the cellular immune function; as shown in tables 2 and 3, the results of the tests on the serum hemolysin detection and the test on the spleen cell antibody production of the sheep red blood cell immunized test animals are positive, indicating that the test samples have the function of enhancing the humoral immunity. The fermented cordyceps composition disclosed by the invention has the function of enhancing the immunity.
TABLE 1 Effect of fermented Cordyceps composition on delayed allergy in mice
TABLE 2 influence of fermented Cordyceps composition on half-maximal hemolysin value of mouse serum hemolysin
TABLE 3 Effect of fermented Cordyceps composition on mouse splenocyte antibody production levels
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent substitutions or changes according to the technical solution and the concept of the present invention should be covered by the scope of the present invention.
Claims (10)
1. A fermented cordyceps composition with an immunity enhancing effect is characterized by comprising the following components in parts by weight: 75-100 parts of paecilomyces hepiali mycelium powder and 0-30 parts of cordyceps militaris.
2. The fermented cordyceps composition with the immunity enhancing effect according to claim 1, which is characterized by comprising the following components in parts by weight: 75-90 parts of paecilomyces hepiali mycelium powder and 10-25 parts of cordyceps militaris powder.
3. A method for preparing the fermented cordyceps composition with immunity enhancing effect according to claim 1 or 2, comprising the steps of:
(1) stirring for moistening, moist heat sterilization and drying: mixing cordyceps militaris fruit bodies according to a material-liquid ratio of 1: 0.1-0.3 of adding water, stirring for moistening for 5-20 min, then carrying out damp-heat sterilization at 121 ℃ for 5-25 min, and drying the moistened cordyceps militaris sporocarp until the water content is less than or equal to 6.5% and the drying temperature is 55-80 ℃;
(2) crushing and sieving: crushing the sterilized and dried cordyceps militaris sporocarp and paecilomyces hepiali mycelium, and sieving the crushed cordyceps militaris sporocarp and paecilomyces hepiali mycelium by a 40-200-mesh sieve for later use;
(3) mixing: uniformly mixing the sieved cordyceps militaris sporocarp powder and the sieved paecilomyces hepiali mycelium powder for 10-50 min to obtain a mixture;
(4) and (3) granulating: granulating a mixture of cordyceps militaris sporocarp powder and paecilomyces hepiali mycelium powder to obtain granules;
(5) filling: and filling the particles into a solid preparation to obtain the fermented cordyceps composition.
4. The preparation method of claim 3, wherein in the step (1), the ratio of the cordyceps militaris fruit body to the liquid material is 1: 0.1-0.2, adding water, stirring for wetting, stirring for 8-15 min, performing damp heat sterilization at 121 ℃ for 10-20 min, and drying the wetted cordyceps militaris sporocarp until the water content is less than or equal to 6.5% and the drying temperature is 60-70 ℃.
5. The preparation method according to claim 3, wherein in the step (2), the sterilized and dried cordyceps militaris sporocarp and paecilomyces hepiali mycelium are crushed and sieved by a sieve of 80-200 meshes for later use.
6. The method according to claim 3, wherein the mixing time in the step (3) is 10 to 30 min.
7. The preparation method according to claim 3, wherein in the step (4), the mixture of the cordyceps militaris sporocarp and the paecilomyces hepiali mycelium powder is added into a dry granulation machine, and granulation is carried out under the pressure of 1-3 MPa to obtain the granules.
8. The preparation method according to claim 3, wherein in the step (5), the granules are filled into a capsule preparation in an amount of 330 mg/granule to obtain the fermented cordyceps composition.
9. Use of the fermented cordyceps composition with immunity enhancing effect according to claim 1 or 2 in the preparation of food or medicine with immunity enhancing effect.
10. The use according to claim 9, wherein the food product comprises functional food, health food; the product forms of the food or the medicine comprise powder, capsules and tablets.
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Application publication date: 20210413 |