CN112640919B - Drosophila attractant prepared from extract of leaves of plant of Acanthopanax - Google Patents
Drosophila attractant prepared from extract of leaves of plant of Acanthopanax Download PDFInfo
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- CN112640919B CN112640919B CN202110105456.4A CN202110105456A CN112640919B CN 112640919 B CN112640919 B CN 112640919B CN 202110105456 A CN202110105456 A CN 202110105456A CN 112640919 B CN112640919 B CN 112640919B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a drosophila attractant prepared from an extract of leaves of a plant in the genus of acanthopanax, which is prepared by the following method: A. placing newly picked leaves of Acanthopanax plant in a foam box with ice bag for low temperature preservation, wherein the ice bag is separated from the leaves by foam; B. taking the leaves out of the foam box, and irradiating for 20-40 minutes under the light of ultraviolet rays; C. placing the ultraviolet-irradiated and sterilized leaves into a wall breaking machine, and adding 50-100 ml of alcohol for wall breaking treatment; D. placing the leaf solution after the wall breaking treatment into active carbon particles, standing for 30 minutes, and filtering with filter paper; E. evaporating the filtered liquid into paste by using a rotary evaporator, and placing the paste in a refrigerator for later use; F. the plant paste is prepared into 20-50% water solution which is sprayed on fruits and vegetables, and has obvious poisoning effect on fruit flies in different periods.
Description
Technical Field
The invention belongs to the field of pest control, and particularly relates to a drosophila attractant prepared from an extract of leaves of a plant of the genus acanthopanax.
Background
Along with the attention of people on the health and safety of food and drink, green organic fruits are more and more popular. The blueberry planting industry in Guizhou is continuously expanded, the problem of blueberry diseases and insect pests is getting more and more serious, and fruit flies (Drosophila) are one of the most serious pests harming blueberry fruits. The fruit flies lay eggs on mature blueberry fruits in rainy and high-temperature seasons, the surfaces of the damaged fruits are slightly sunken, worm holes with the sizes of needle points are formed in the surfaces of the fruits, hatched larvae eat the fruits and are damaged, and insect dung exists in the fruits; damaged fruits become soft, juice overflows and falls, so that the yield is reduced and the quality is deteriorated. Meanwhile, the safety, commodity value, fresh sale, storage, processing and the like of fruit damaged by fruit flies are seriously affected.
Besides the blueberries, fruit flies can also harm various fruits such as cherries, grapes, raspberries and blackberries, and seriously affect the quality of the fruits and the economic income of fruit growers.
Therefore, how to provide a drosophila attractant which has good control effect, no pollution to fruits and no pollution to the environment becomes a technical problem which needs to be solved by the technical personnel in the field. In order to prevent fruit flies from being harmed by adding natural plants, effectively reduce the adding amount of the artificially synthesized insect-resist agent and further effectively ensure the food safety, the inventor selects acanthopanax plants as experiments.
Acanthopanax (academic name:Acanthopanaxmiq.) is a genus of Araliaceae, deciduous shrub or small tree. There are currently 37 (not including varieties) plants of the araliaceae family, araliaceae, which are distributed mainly in asia. The Chinese has 26 varieties of 18 varieties, which account for the first place in the world, are widely distributed in various provinces of China, and are the most abundant in Yangtze river basin, wherein the most common varieties are acanthopanax gracilistylus, acanthopanax senticosus and acanthopanax giraldii harms. The plants of Acanthopanax have medicinal value, and have antiinflammatory, anti-stress, antiarrhythmic, platelet aggregation inhibiting, antitumor, and analeptic effectsSmooth muscle action, antiulcer action, etc., which affect the immune system and metabolism of substances.
Disclosure of Invention
The invention aims to provide a drosophila trapping and killing agent prepared from acanthopanax plant leaf extract, which acts on drosophila in different periods and has obvious poisoning and killing effects.
The purpose of the invention and the main technical problem of solving the invention are realized by adopting the following technical scheme:
a drosophila attractant prepared from acanthopanax plant leaf extract is prepared by the following steps:
A. placing newly picked leaves of Acanthopanax plant in a foam box with ice bag for low temperature preservation, wherein the ice bag is separated from the leaves by foam;
B. taking the leaves out of the foam box, and irradiating for 20-40 minutes under the light of ultraviolet rays;
C. placing the leaves sterilized by ultraviolet irradiation into a wall breaking machine, and adding 50-100 ml of alcohol for wall breaking treatment;
D. placing the solution of the leaves after the wall breaking treatment into active carbon particles, standing for 20-30 minutes, and filtering with filter paper;
E. evaporating the filtered liquid into paste by using a rotary evaporator, and placing the paste in a refrigerator for later use;
F. the plant paste is prepared into 20-50% water solution which is sprayed on fruits and vegetables, and has obvious poisoning effect on fruit flies in different periods.
Further, in the step A, the acanthopanax plant leaves comprise acanthopanax sessiliflorus leaves, acanthopanax senticosus leaves, acanthopanax hollyhock leaves, acanthopanax senticosus leaves and acanthopanax evodia leaves.
Further, in the step B, the irradiation time is 30 minutes.
Further, in the step D, the standing time is 30 minutes.
Compared with the prior art, the invention has obvious advantages and beneficial effects. According to the technical scheme, the invention has the following characteristics:
1. step A, preventing leaves from freezing and ensuring the activity of fresh leaves;
2. b, irradiating the leaves with ultraviolet light to prevent bacteria carried on the plant leaves from influencing the poisoning effect of the fruit flies;
3. step C, adding alcohol during the wall breaking treatment of the leaves, so that the leaves can be fully broken, the sterilization effect can be achieved, and meanwhile, the leaves are treated by a wall breaking machine, so that the leaves can be changed into small particles, and active substances in the leaves can be better released;
4. step D, the crude extract of the leaves is prepared into paste, so that the paste is more convenient to store, and the experiment is not limited by seasonal changes; and secondly, when the aqueous solution is prepared by using the leaf paste, the error of the water content of the leaves on the experiment can be reduced.
Detailed Description
The following detailed description is provided for the specific embodiments, features and functions of the present invention in connection with the preferred embodiments.
In the previous experiments, most of drosophila adults are used as experimental objects, because the drosophila belongs to small-body insects, the volumes of eggs, larvae and pupae of the drosophila are smaller, the experiment utilizes a low-temperature anesthesia method to firstly perform male and female separation after the female and male drosophila are anesthetized, then 10 male and female drosophila are selected and are placed in a culture medium again to be cultured for 4 hours, and then the drosophila is taken out.
A drosophila attractant prepared from acanthopanax plant leaf extract is characterized in that: it is prepared by the following method:
A. placing newly picked leaves of Acanthopanax plant in a foam box with ice bag for low temperature preservation, wherein the ice bag is separated from the leaves by foam;
B. taking the leaves out of the foam box, and irradiating for 20-40 minutes under the light of ultraviolet rays;
C. placing the leaves sterilized by ultraviolet irradiation into a wall breaking machine, and adding 50-100 ml of alcohol for wall breaking treatment;
D. placing the solution of the leaves after the wall breaking treatment into active carbon particles, standing for 20-30 minutes, and filtering with filter paper;
E. evaporating the filtered liquid into paste by using a rotary evaporator, and placing the paste in a refrigerator for later use;
F. the plant paste is prepared into 20-50% water solution which is sprayed on fruits and vegetables, and has obvious poisoning effect on fruit flies in different periods.
The invention is illustrated below by means of several examples:
example 1:
1. placing newly picked leaves of Acanthopanax gracilistylus W.W.W. in a foam box with ice bag for low temperature preservation, wherein the ice bag is separated from the leaves by foam;
2. taking the leaves out of the foam box, and irradiating for 30 minutes under the light of ultraviolet rays;
3. placing the ultraviolet-irradiated and sterilized leaves into a wall breaking machine, and adding a small amount of alcohol for wall breaking;
4. placing the leaf solution after the wall breaking treatment into active carbon particles, standing for a while, and filtering with filter paper;
5. evaporating the filtered liquid into paste by using a rotary evaporator to obtain a crude extract, and placing the crude extract in a refrigerator for later use;
6. placing 9g of agar and 100g of sucrose in a pot, adding 500ml of distilled water, boiling, and continuously stirring until the agar is completely dissolved; adding 75g corn flour into a measuring cup, dissolving with 500ml distilled water, stirring to paste, slowly pouring into an agar pan, decocting for 1min, removing fire source, adding 4g yeast powder, stirring, cooling to 60 deg.C, adding 4ml propionic acid, and making into culture medium. The experiment uses drosophila, the temperature is 28 ℃, the relative humidity is 60%, and the photoperiod light/dark =12h/12 h. The prepared culture medium is filled in a test tube with the model number 15 x 100, the test tube is placed to normal temperature by inclining 20 degrees, adult fruit flies are put in the test tube and cultured for 15 days, then all the adult fruit flies are taken out and placed in other culture mediums, newly emerged fruit flies are taken out every other hour from the original culture medium, the taken-out fruit flies are placed in a wide-mouth bottle, the wide-mouth bottle is placed in an ice-water mixture, ice is supplemented properly in the experimental process on the basis that the ice-water mixture does not just fall into the wide-mouth bottle, the test tube is taken out after the temperature is controlled to be not more than 1 ℃ by a thermometer, and the adult male and female fruit flies are distinguished and filled in the new test tube filled with the culture medium. Then 10 male and female fruit flies are selected and put into the culture medium again for culturing for 4 hours, adult fruit flies are taken out, and test tubes after spawning are continuously cultured, so that fruit fly larvae, fruit fly pupae and fruit fly adults in the same growth period can be obtained.
F. The acanthopanax senticosus leaf paste is prepared into an aqueous solution according to 20 percent, and 20 pieces of the acanthopanax senticosus leaf paste aqueous solution are sprayed every day after taking out the drosophila ova, drosophila larvae, drosophila pupae and drosophila imagoes in the same growth period.
Example 2: the method of example 1 is used to obtain the fruit flies and the water solution of the acanthopanax gracilistylus leaf paste in different stages of each growth period of the same growth period, and the water solution of the acanthopanax gracilistylus leaf paste is used to spray the fruit flies in different periods.
Example 3: the method of example 1 was used to obtain the aqueous solution of the drosophila melanogaster and the acanthopanax hollyhock leaf paste at different stages of each growth period of the same growth period, and the aqueous solution of the acanthopanax hollyhock leaf paste was used to spray the drosophila melanogaster at different stages.
Example 4: the method of example 1 was used to obtain aqueous solutions of drosophila melanogaster and acanthopanax leaf paste at different stages of each growth period of the same growth period, and the aqueous solutions of acanthopanax leaf paste were used to spray the drosophila melanogaster at different stages.
Example 5: the method of example 1 was used to obtain the aqueous solution of drosophila melanogaster and acanthopanax evodiae leaf extract at different stages of each growth period of the same growth period, and the aqueous solution of acanthopanax evodiae leaf extract was used to spray the drosophila melanogaster at different periods.
The control group was sprayed with water to obtain fruit flies at different stages of each growth period of the same growth period by the method of example 1.
For the results recorded for examples 1-5 above and for the control group, the experimental data are shown in table 1:
the fruit fly poisoning agent has obvious poisoning effect when acting on fruit flies in different periods, and natural plants are added to prevent the fruit flies from being damaged, so that the addition of the artificially synthesized insect-resist agent is effectively reduced, and the food safety is further effectively ensured.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the present invention without departing from the technical spirit of the present invention.
Claims (4)
1. A drosophila attractant prepared from acanthopanax plant leaf extract is characterized in that: it is prepared by the following method:
A. placing newly picked leaves of Acanthopanax plant in a foam box with ice bag for low temperature preservation, wherein the ice bag is separated from the leaves by foam;
B. taking the leaves out of the foam box, and irradiating for 20-40 minutes under the light of ultraviolet rays;
C. placing the leaves sterilized by ultraviolet irradiation into a wall breaking machine, and adding 50-100 ml of alcohol for wall breaking treatment;
D. placing the solution of the leaves after the wall breaking treatment into active carbon particles, standing for 20-30 minutes, and filtering with filter paper;
E. evaporating the filtered liquid into paste by using a rotary evaporator, and placing the paste in a refrigerator for later use;
F. the plant paste is prepared into 20-50% aqueous solution which is sprayed on fruits and vegetables, and the fruit and vegetable pesticide has obvious poisoning effect on fruit flies in different periods;
the Acanthopanax plant leaves are Acanthopanax gracilistylus leaves, Acanthopanax hollyx leaves, Acanthopanax senticosus leaves and Acanthopanax evodiae leaves.
2. The drosophila attractant prepared from the extract of the leaves of the plant of the acanthopanax genus as claimed in claim 1, wherein: and in the step B, the irradiation time is 30 minutes.
3. The drosophila attractant prepared from the extract of the leaves of the plant of the acanthopanax genus as claimed in claim 1, wherein: and D, standing for 30 minutes.
4. The drosophila attractant prepared from the extract of the leaves of the plant of the acanthopanax genus as claimed in claim 1, wherein: and F, preparing the plant paste into an aqueous solution according to 20%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110105456.4A CN112640919B (en) | 2021-01-26 | 2021-01-26 | Drosophila attractant prepared from extract of leaves of plant of Acanthopanax |
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| CN202110105456.4A CN112640919B (en) | 2021-01-26 | 2021-01-26 | Drosophila attractant prepared from extract of leaves of plant of Acanthopanax |
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| CN112640919A CN112640919A (en) | 2021-04-13 |
| CN112640919B true CN112640919B (en) | 2021-07-23 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007002960A3 (en) * | 2005-06-28 | 2007-05-24 | Schalk Francois Mouton | Composition and method of pest control |
| CN101152510A (en) * | 2007-09-12 | 2008-04-02 | 王�锋 | Pharmaceutical composition, preparing method and use the same |
| CN103356724A (en) * | 2012-08-22 | 2013-10-23 | 东北林业大学 | Application of acanthopanax water extract in relieving intestinal inflammation of drosophila melanogaster |
| JP5345280B2 (en) * | 2006-07-14 | 2013-11-20 | アース製薬株式会社 | Spray products for controlling fly flies |
| CN108513994A (en) * | 2018-05-15 | 2018-09-11 | 河北农业大学 | Drosophila attractant and preparation method and use method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160081352A1 (en) * | 2014-09-23 | 2016-03-24 | Luis Augusto Mazariegos | Pest control formulation of Neem and Beauveria bassiana and methods of making and using same |
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2021
- 2021-01-26 CN CN202110105456.4A patent/CN112640919B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007002960A3 (en) * | 2005-06-28 | 2007-05-24 | Schalk Francois Mouton | Composition and method of pest control |
| JP5345280B2 (en) * | 2006-07-14 | 2013-11-20 | アース製薬株式会社 | Spray products for controlling fly flies |
| CN101152510A (en) * | 2007-09-12 | 2008-04-02 | 王�锋 | Pharmaceutical composition, preparing method and use the same |
| CN103356724A (en) * | 2012-08-22 | 2013-10-23 | 东北林业大学 | Application of acanthopanax water extract in relieving intestinal inflammation of drosophila melanogaster |
| CN108513994A (en) * | 2018-05-15 | 2018-09-11 | 河北农业大学 | Drosophila attractant and preparation method and use method thereof |
Non-Patent Citations (3)
| Title |
|---|
| Acanthopanax senticosus polysaccharide regulates the intestinal homeostasis disruption induced by toxic chemicals in Drosophila;Zhang, Hong等;《PHYTOTHERAPY RESEARCH》;20200131;第34卷(第1期);第193-200页 * |
| Toxicity of medicinal plant extracts to Lycoriella ingenua (Diptera Sciaridae) and Coboldia fuscipes (Diptera Scatopsidae);Yi, Jee-Hwan等;《Journal of Asia-Pacific Entomology》;20081231;第11卷(第4期);第221-223页 * |
| 刺五加总苷及刺五加苷B对持续黑暗条件下果蝇睡眠-觉醒节律的量效关系研究;卞宏生等;《中国药物依赖性杂志》;20200615;第29卷(第3期);第231-236页 * |
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