CN112625978A - Banana growth-promoting water-soluble microbial agent, preparation method and application thereof - Google Patents
Banana growth-promoting water-soluble microbial agent, preparation method and application thereof Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/27—Pseudomonas
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A01N63/38—Trichoderma
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a banana growth promoting water-soluble microbial agent, a preparation method and application thereof, wherein a compound comprises six microorganisms, and consists of pseudomonas aeruginosa, serratia marcescens, podobule burkholderia, pseudomonas fluorescens, bacillus subtilis and trichoderma viride, and the preparation method comprises the following steps: preparing water-soluble carbon source from rice flour processing waste; culturing six kinds of bacteria in a water-soluble carbon source to prepare six liquid microbial agents; and (3) air-drying and mixing the six liquid microbial agents to obtain the water-soluble compound microbial agent. The invention relates to the technical fields of waste utilization, agricultural microorganisms, ecological planting and microbial agents. The water-soluble microbial agent for promoting growth of bananas, the preparation method and the application thereof can recycle rice flour waste, not only can reduce the cost of industrial production, avoid waste of waste resources, but also can reduce the pollution of the waste to the environment, improve the yield of bananas and reduce the plant diseases and insect pests of bananas.
Description
Technical Field
The invention relates to the technical fields of waste utilization, agricultural microorganisms, ecological planting and microbial agents, in particular to a banana growth-promoting water-soluble microbial agent, a preparation method and application thereof.
Background
At present, the rice flour processing industry in China has large discharge amount of wastewater, and the pollutants mainly to be removed in the wastewater are rice flour carbohydrates, are easy to biodegrade, and can be used as a cheap carbon source for microbial culture. The phenomenon of chemical fertilizer abuse in the planting industry is common, and water bodies polluted by the chemical fertilizer are finally gathered into a human body through ecological circulation, so that the drug resistance of bacteria in the human body is increased, and the health of the human body is damaged. The banana has developed root system and large fertilizer demand, and the supply of nutrient elements is needed in the whole growth process.
The fertilizer and pesticide has zero growth requirement for the agricultural department of corresponding country. To achieve zero growth of chemical fertilizers and pesticides and to ensure the yield and income increase of bananas and to achieve effective supply on the supply side, products capable of effectively replacing chemical fertilizers and pesticides are needed. Therefore, microbial preparations capable of replacing chemical fertilizers and reducing pesticides are in urgent need of developing ecological agriculture at present, the microbial preparations are used for improving planting environment, improving product quality and realizing yield increase, the microbial preparations are a necessary trend of modern planting, and the microbial preparations are a necessary choice for developing green sustainable efficient ecological agriculture.
The beneficial microorganism bacteria are added into the fertilizer, so that the digestion and absorption of the banana plants to the nutrition in the soil can be promoted, and the using amount of the fertilizer can be greatly reduced; microbial beneficial bacteria are added into the drip irrigation water, a microbial community system is changed, the growth promotion and disease resistance of bananas are enhanced, the fertilizer water and pesticide consumption can be greatly reduced, and the ecological water environment is restored and improved.
In recent years, various microbial fertilizers are abundant like bamboo shoots in spring after rain, but products with obvious fertilizer efficiency reaching or exceeding the fertilizer are rare, and the main reasons are as follows: firstly, the microbial strain is single, and various problems of crop growth cannot be solved; secondly, the storage period of the microbial inoculum is short, the fertilizer effect is not obvious, the problems of putrefaction, death of effective bacteria and short storage period of the liquid microbial inoculum are difficult to solve for both single microbial inoculum and composite microbial inoculum, the conventional microbial inoculum is propagated for a generation in a liquid state for several minutes to several hours, the strain is rapidly malformed and died due to continuous rapid propagation and growth, the microbial inoculum is blackened and smelly, most floras of the microbial inoculum are malformed and weak after dilution, the effective functions of the microbial inoculum cannot be exerted, and the storage period is short under the normal temperature condition and is generally only several months.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a banana growth promoting water-soluble microbial agent, a preparation method and application thereof.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a water-soluble microbial agent for promoting growth of bananas is prepared from a culture medium culture microbial agent of rice flour processing waste, wherein the water-soluble microbial agent comprises six liquid microbial agents cultured by using a liquid culture medium, a carbon source of the culture medium of the liquid microbial agent is from the rice flour processing waste, and each 10 parts by mass of the liquid microbial agent comprises the following microbial agents in parts by mass:
1-3 parts of pseudomonas aeruginosa agent;
1-3 parts of serratia marcescens agent;
1 part by mass of a Burkholderia glumae agent;
1-2 parts of liquid bacillus subtilis preparation;
1-2 parts of pseudomonas fluorescens agent;
1-2 parts of trichoderma viride;
wherein the content of the good-culture bacteria in each liquid microbial inoculum is more than or equal to 2 multiplied by 108 cfu/mL.
Preferably, the microbial liquid inoculum further comprises trace elements, the addition amount of the trace elements is 0.1-0.2% of the mass of the composite microbial liquid inoculum, and the trace elements are selected from any one or a mixture of at least two of B, Zn, Mn, Cu and Co.
The invention also discloses a preparation method of the banana growth promoting water-soluble microbial agent, which comprises the following steps:
s1, utilizing the starch content in the rice flour processing wastewater, recycling the rice flour processing waste as a main carbon source of microorganisms to replace a carbon source commonly used in the traditional industry, reducing the production cost, collecting the wastewater generated by rice flour processing treatment in a recovery tank, standing for precipitation, removing supernatant, collecting precipitate, adding a proper amount of clear water for dissolution, filtering to remove large-particle solids, and air-drying and recycling the solution as the carbon source of a liquid culture medium;
s2, respectively culturing the six bacteria in the claim 1 to prepare six liquid bacteria agents;
s3, slowly injecting the six liquid microbial inoculum into a compound culture medium according to the formula proportion of claim 1, uniformly stirring, shaking, and after homogenization and stabilization, air-drying to obtain the water-soluble compound microorganism liquid microbial inoculum.
Preferably, in step S2, the six bacteria in claim 1 are cultured respectively to obtain six liquid microbial inoculum, and the specific steps are as follows:
1, respectively preparing liquid bacteria culture media of pseudomonas aeruginosa, serratia marcescens, podbokholderia glumae, pseudomonas fluorescens, bacillus subtilis and trichoderma viride, respectively inoculating the strains which are respectively correspondingly activated into the six liquid bacteria culture media, culturing for 12 hours at the temperature of 28-35 ℃, the temperature of 120-;
2, respectively taking six primary bacterial liquids, inoculating respective corresponding strains into six liquid bacterial culture media, carrying out secondary amplification culture on the strains at the temperature of 28-35 ℃, at the temperature of 120-140r/min and under the condition of pH value of 5.5-7.5, and culturing for 8-10h to obtain six secondary bacterial liquids, wherein the inoculation amount of each primary bacterial liquid is 0.9-2.5% of the mass of the liquid bacterial culture medium;
3, inoculating the strains corresponding to the six secondary bacterial liquids into the six liquid bacterial culture media, carrying out three-stage amplification culture on the strains at the temperature of 28-37 ℃, the temperature of 120-140r/min and the pH value of 5.5-7.5, and culturing for 8h to obtain the six liquid bacterial agents, wherein the inoculation amount of each secondary bacterial liquid is 1-3.5% of the mass of the liquid culture medium.
Preferably, in step S3, trace elements are added simultaneously, the addition amount of the trace elements is 0.001-0.15% of the mass of the composite microbial liquid inoculum, and the trace elements are selected from any one or a mixture of at least two of B, Zn, Mn, Cu and Co.
Preferably, the pseudomonas aeruginosa liquid culture medium formula consists of the following components in percentage by mass: 3-10% of rice flour production waste, 2-8% of soybean meal, 0.1-0.6% of dipotassium phosphate, 0.2-0.8% of ammonium sulfate, 0.3-0.6% of calcium carbonate and sterile water;
the liquid bacterium culture medium formula of the serratia marcescens agent comprises the following components in percentage by mass: 2-10% of rice flour production waste, 1-8% of soybean meal, 0.1-0.8% of dipotassium phosphate, 0.5% of yeast extract, 0.01-0.03% of magnesium sulfate, 0.2-0.7% of sodium chloride and sterile water;
the liquid bacteria culture medium formula of the Burkholderia glumae comprises the following components in percentage by mass: 2-8% of rice flour production waste, 2-8% of soybean meal, 0.04-0.18% of dipotassium phosphate, 0.15-0.2% of magnesium sulfate and sterile water;
the liquid bacterium culture medium formula of the pseudomonas fluorescens agent comprises the following components in percentage by mass: 1.5-10% of rice flour production waste, 2-8% of soybean meal, 0.2-0.5% of sodium chloride, 0.05-0.1% of dipotassium phosphate, 0.05-0.1% of potassium nitrate, 0.03-0.08% of magnesium sulfate, 0.001-0.005% of ferrous sulfate and sterile water;
the liquid bacterium culture medium formula of the bacillus subtilis comprises the following components in percentage by mass: 2-10% of rice flour production waste, 1.5-10% of soybean meal, 0.05-0.2% of sodium chloride, 0.1-0.2% of dipotassium phosphate, 0.05-0.015% of calcium carbonate, 0.001-0.005% of manganese sulfate, 0.001-0.003% of ferrous sulfate and sterile water;
the liquid fungus culture medium formula of the trichoderma viride comprises the following components in percentage by mass: 2-10% of rice flour production waste, 1-4% of molasses, 1.5-8% of soybean meal, 0.1-0.2% of dipotassium phosphate, 0.05-0.1% of calcium carbonate, 0.05-0.5% of sodium chloride, 0.001-0.03% of manganese sulfate and sterile water.
The invention also discloses an application of the banana growth promoting water-soluble microbial agent in banana planting by taking rice flour processing waste as a raw material.
The design characteristics of the invention are as follows: the banana growth promoting microbial agent taking rice flour processing waste as a raw material is a microbial liquid microbial agent formed by compounding floras, and is characterized in that the rice flour processing waste is prepared into a carbon source respectively, the microbial agent consists of six bacteria and facultative bacteria, and each bacteria takes the carbon source as the raw material and is fermented independently by adopting a fermentation process. Finally, mixing the six bactericides according to the proportion respectively. The characteristics have the advantages that the six strains are independently cultured in the fermentation stage, and the quick growth and propagation of the strains can be promoted on the premise of ensuring the purity and functionality of the strains. After fermentation is completed, the growth and reproduction become slow due to the inhibition of weak acid conditions, spores are formed by most strains, excellent symbiotic environmental conditions which are not antagonistic to each other are formed among the strains, and the solid microbial inoculum can be stored for a long time at normal temperature.
The compound contains six microorganisms, and consists of Pseudomonas aeruginosa (Pseudomonas aeruginosa), Serratia marcescens (Serratia marcescens) with the collection number of CGMCC 1.12941, Burkholderia glumae (Burkholderia tubericum) with the collection number of CGMCC 1.10205, Pseudomonas fluorescens (Pseudomonas fluorescens) with the collection number of CGMCC 1.4525, Bacillus subtilis with the collection number of laboratory and Trichoderma viride (Trichoderma viride) with the collection number of CGMCC 3.11450.
(III) advantageous effects
The invention provides a banana growth promoting water-soluble microbial agent, a preparation method and application thereof. Compared with the prior art, the method has the following beneficial effects:
(1) the banana growth promoting water-soluble microbial agent, the preparation method and the application thereof have the advantages that the composite microbial liquid agent has more effective viable bacteria and long storage period, six microbial agents are fermented respectively and then compounded in proportion, the growth condition of the flora is inhibited in a weakly acidic environment after compounding, meanwhile, nutrients and metabolic energy are complemented, excellent symbiotic environment with interdependence and non-antagonism is formed among floras, the microbial inoculum adopts natural air drying, only water is evaporated, the number of thalli and metabolic products are retained to the maximum extent, therefore, although the growth of the floras is inhibited, the activity of the bacterial strain is retained, under the condition of normal temperature, the storage period of the banana growth promoting water-soluble microbial agent taking the rice flour processing waste as the raw material can reach more than 1 year, and after the banana growth promoting water-soluble microbial agent is dissolved and diluted by water, the bacterial strain can quickly recover to grow and play a role.
(2) The composite microbial liquid agent has comprehensive functions, and can enable more strains with different functions to be compounded and symbiotic by adopting a method of respectively fermenting 6 flora agents and then compounding the 6 flora agents in proportion, so that the composite microbial liquid agent can decompose pollutants, purify water quality, generate immune metabolites, inhibit bacteria, resist insects, prevent diseases such as banana wilt and the like after being applied, remarkably improve the quality of bananas, and create favorable conditions for realizing biological treatment of water pollution, improving ecological environment and creating favorable conditions.
Drawings
FIG. 1 is a flow chart of a preparation method of the banana growth promoting water-soluble microbial agent of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, the embodiment of the present invention provides three technical solutions: a banana growth promoting water-soluble microbial agent, a preparation method and an application thereof specifically comprise the following embodiments:
example 1
1 kg of water is added into 1 g of the microbial inoculum to prepare a bacterial solution, 0.2ml of the bacterial solution is taken and coated on an LB solid culture medium, and 0.2ml of physiological saline is taken as a control and coated on five plates respectively. Fusarium oxysporum was inoculated in the center of each plate and cultured at 27 ℃. After 24h, the experimental group was observed to have a significant inhibition of the growth of Fusarium oxysporum, while the control group had plates full of Fusarium oxysporum colonies.
Example 2
30 g of the fungicide is mixed with 30 kg of water to prepare a spray-treated 20-square banana seedbed, and the 20-square banana seedbed is treated by using a common fertilizer as a reference. And spraying Fusarium oxysporum for treating banana wilt. After the banana seedlings are continuously used for one month, the prevalence rate of the banana seedlings in the experimental group is only 2%, which is obviously lower than that of 37% in the control group, the banana seedlings are greener and stronger, the average growth height is 5cm higher than that of the control group, and the root systems are more vigorous.
Example 3
One hundred acre of the plot was divided into two parts in the east-west direction, wherein one part was selected as the a plot (fifty acres occupied) to use the composite microbial preparation of the present invention, and the other plot was the B plot as a control. The composite microbial liquid inoculum of example 2 was used in park a by the following method: 1. 1% of the microbial inoculum is added into farmyard manure; 2. 1% of the microbial inoculum is added into drip irrigation water; 3. diluting the microbial inoculum by 20 times with clear water, and spraying the microbial inoculum on the soil surface of the banana garden 1 time per day; 4. 1 percent of the microbial inoculum is mixed and poured into a manure pool every day. After the banana tree is continuously used for 1 month, the growth state of bananas in the area A is obviously improved compared with that in the area B, the banana tree is obviously lower than seedlings of banana infectious diseases and nematodes in the area B after being detected by workers, roots of the banana tree are more vigorous, the growth height of the banana tree is higher, the chlorophyll content in leaves of the banana tree is higher, and the maturing rate of the banana tree is higher.
The composite microbial liquid microbial agent has a large number of effective viable bacteria and a long storage period, six microbial agents are fermented respectively and then compounded in proportion, the growth condition of floras is inhibited in a weakly acidic environment after compounding, simultaneously the nutrient and metabolic energy are complemented, a good symbiotic environment with dependence symbiosis and mutual antagonism is formed among the floras, the microbial agent is naturally air-dried, only moisture is evaporated, the number of the bacteria and metabolites are kept to the maximum extent, therefore, although the growth of the floras is inhibited, the vitality of the bacterial strain is kept, the storage validity period of the banana growth promoting water-soluble microbial agent taking rice flour processing waste as a raw material can reach more than 1 year under the normal temperature condition, the bacterial strain can quickly recover growth and play a role after being dissolved and diluted by adding water, and meanwhile, the multiple strains in the composite microbial liquid microbial agent are synergized, the functions are comprehensive, and by adopting the method of respectively fermenting 6 flora microbial agents and then compounding the microbial agents in proportion, more strains with different functions can be compounded and symbiotic, after the microbial agents are applied, the effects of decomposing pollutants, purifying water quality, generating immune metabolites, inhibiting bacteria, resisting insects, preventing diseases such as banana wilt and the like are achieved, the quality of bananas is remarkably improved, and favorable conditions are created for realizing biological treatment of water pollution, improving ecological environment and improving ecological environment.
The crops can be topdressed by water flushing and drop irrigation at any time in the whole growth period, a large amount of beneficial bacteria in the soil can be quickly supplemented, the ineffective phosphorus and potassium in the soil can be dissolved, the soil structure can be improved, and the fertilizer efficiency can be increased. The microbial secondary metabolite produced by the product has strong water and fertilizer retention functions, can inhibit the growth and propagation of harmful bacteria, reduce the occurrence probability of soil diseases and banana diseases, stimulate the development of root systems, quickly rejuvenate crops, improve the quality, prolong the harvesting period, and is economic and environment-friendly.
And those not described in detail in this specification are well within the skill of those in the art.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A water-soluble microbial agent for promoting growth of bananas is characterized in that: the raw material of the microbial agent is a culture medium culture microbial agent of rice flour processing waste, the water-soluble microbial agent comprises six liquid microbial agents cultured by using a liquid culture medium, a carbon source of the culture medium of the liquid microbial agent is from the rice flour processing waste, and each 10 parts by mass of the liquid microbial agent is composed of the following microbial agents in parts by mass:
1-3 parts of pseudomonas aeruginosa agent;
1-3 parts of serratia marcescens agent;
1 part by mass of a Burkholderia glumae agent;
1-2 parts of liquid bacillus subtilis preparation;
1-2 parts of pseudomonas fluorescens agent;
1-2 parts of trichoderma viride;
wherein the content of the good-culture bacteria in each liquid microbial inoculum is more than or equal to 2 multiplied by 108 cfu/mL.
2. The water-soluble microbial agent for promoting banana growth, the preparation method and the application thereof according to claim 1, wherein the water-soluble microbial agent for promoting banana growth is characterized in that: the microbial liquid inoculant further comprises trace elements, the addition amount of the trace elements is 0.1-0.2% of the mass of the composite microbial liquid inoculant, and the trace elements are selected from any one or a mixture of at least two of B, Zn, Mn, Cu and Co.
3. A method for preparing the banana growth promoting water-soluble microbial agent of claim 1 or 2, wherein: the method specifically comprises the following steps:
s1, collecting wastewater generated in the rice flour processing treatment in a recovery tank, standing for precipitation, removing supernatant, collecting precipitate, adding a proper amount of clear water for dissolution, filtering to remove large-particle solids, and air-drying the solution for recovery as a carbon source of a liquid culture medium;
s2, respectively culturing the six bacteria in the claim 1 to prepare six liquid bacteria agents;
s3, slowly injecting the six liquid microbial inoculum into a compound culture medium according to the formula proportion of claim 1, uniformly stirring, shaking, and after homogenization and stabilization, air-drying to obtain the water-soluble compound microorganism liquid microbial inoculum.
4. The preparation method of the banana growth promoting water-soluble microbial agent according to claim 3, wherein the preparation method comprises the following steps: in step S2, the six bacteria in claim 1 are respectively cultured to prepare six liquid microbial inoculum, and the specific steps are as follows:
1, respectively preparing liquid bacteria culture media of pseudomonas aeruginosa, serratia marcescens, podbokholderia glumae, pseudomonas fluorescens, bacillus subtilis and trichoderma viride, respectively inoculating the strains which are respectively correspondingly activated into the six liquid bacteria culture media, culturing for 12 hours at the temperature of 28-35 ℃, the temperature of 120-;
2, respectively taking six primary bacterial liquids, inoculating respective corresponding strains into six liquid bacterial culture media, carrying out secondary amplification culture on the strains at the temperature of 28-35 ℃, at the temperature of 120-140r/min and under the condition of pH value of 5.5-7.5, and culturing for 8-10h to obtain six secondary bacterial liquids, wherein the inoculation amount of each primary bacterial liquid is 0.9-2.5% of the mass of the liquid bacterial culture medium;
3, inoculating the strains corresponding to the six secondary bacterial liquids into the six liquid bacterial culture media, carrying out three-stage amplification culture on the strains at the temperature of 28-37 ℃, the temperature of 120-140r/min and the pH value of 5.5-7.5, and culturing for 8h to obtain the six liquid bacterial agents, wherein the inoculation amount of each secondary bacterial liquid is 1-3.5% of the mass of the liquid culture medium.
5. The preparation method of the banana growth promoting water-soluble microbial agent according to claim 3, wherein the preparation method comprises the following steps: in the step S3, trace elements are added at the same time, the addition amount of the trace elements is 0.001-0.15% of the mass of the composite microbial liquid inoculum, and the trace elements are selected from any one or a mixture of at least two of B, Zn, Mn, Cu and Co.
6. The preparation method of the banana growth promoting water-soluble microbial agent according to claim 3, wherein the preparation method comprises the following steps: the formula of the pseudomonas aeruginosa liquid culture medium comprises the following components in percentage by mass: 3-10% of rice flour production waste, 2-8% of soybean meal, 0.1-0.6% of dipotassium phosphate, 0.2-0.8% of ammonium sulfate, 0.3-0.6% of calcium carbonate and sterile water;
the liquid bacterium culture medium formula of the serratia marcescens agent comprises the following components in percentage by mass: 2-10% of rice flour production waste, 1-8% of soybean meal, 0.1-0.8% of dipotassium phosphate, 0.5% of yeast extract, 0.01-0.03% of magnesium sulfate, 0.2-0.7% of sodium chloride and sterile water;
the liquid bacteria culture medium formula of the Burkholderia glumae comprises the following components in percentage by mass: 2-8% of rice flour production waste, 2-8% of soybean meal, 0.04-0.18% of dipotassium phosphate, 0.15-0.2% of magnesium sulfate and sterile water;
the liquid bacterium culture medium formula of the pseudomonas fluorescens agent comprises the following components in percentage by mass: 1.5-10% of rice flour production waste, 2-8% of soybean meal, 0.2-0.5% of sodium chloride, 0.05-0.1% of dipotassium phosphate, 0.05-0.1% of potassium nitrate, 0.03-0.08% of magnesium sulfate, 0.001-0.005% of ferrous sulfate and sterile water;
the liquid bacterium culture medium formula of the bacillus subtilis comprises the following components in percentage by mass: 2-10% of rice flour production waste, 1.5-10% of soybean meal, 0.05-0.2% of sodium chloride, 0.1-0.2% of dipotassium phosphate, 0.05-0.015% of calcium carbonate, 0.001-0.005% of manganese sulfate, 0.001-0.003% of ferrous sulfate and sterile water;
the liquid fungus culture medium formula of the trichoderma viride comprises the following components in percentage by mass: 2-10% of rice flour production waste, 1-4% of molasses, 1.5-8% of soybean meal, 0.1-0.2% of dipotassium phosphate, 0.05-0.1% of calcium carbonate, 0.05-0.5% of sodium chloride, 0.001-0.03% of manganese sulfate and sterile water.
7. The use of the banana growth promoting water-soluble microbial inoculant according to claim 1 in banana planting by using rice flour processing waste as a raw material.
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