CN112624842A - Method for preparing seaweed compound fertilizer - Google Patents
Method for preparing seaweed compound fertilizer Download PDFInfo
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- CN112624842A CN112624842A CN202011523555.6A CN202011523555A CN112624842A CN 112624842 A CN112624842 A CN 112624842A CN 202011523555 A CN202011523555 A CN 202011523555A CN 112624842 A CN112624842 A CN 112624842A
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- seaweed
- enzymolysis
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
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- Pest Control & Pesticides (AREA)
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Abstract
The invention belongs to the technical field of plant fertilizer production. Aiming at the technical problem of overhigh cost of the seaweed compound fertilizer, the invention provides a method for preparing the seaweed compound fertilizer, which comprises the following steps: adding the seaweed into the enzymolysis liquid for enzymolysis, taking the seaweed liquid after enzymolysis to compound with other fertilizers to prepare the seaweed compound fertilizer, and is characterized in that: the seaweed liquid compounded with other fertilizers is prepared by taking supernatant generated after seaweed enzymolysis and performing enzyme reduction treatment on the supernatant. The enzyme recovery method adopted by the invention reduces the waste of energy and enzyme, the enzyme extraction ratio is 12-15%, and the enzyme production cost is greatly reduced.
Description
Technical Field
The invention belongs to the technical field of plant fertilizer production, and particularly relates to a method for preparing a seaweed compound fertilizer.
Background
The seaweed compound fertilizer is a fertilizer which is prepared by preparing a basic fertilizer by using marine brown algae through enzymolysis and then compounding a certain amount of nitrogen, phosphorus and potassium and medium trace elements. The liquid and powder are mainly used as main materials in the market, and a small part of the liquid and powder is in a granular state. At present, most of the processes for producing seaweed compound fertilizers in China use the technology of dry kelp powder enzymolysis and compounding. The method is simple and efficient, but the cost for preparing the basic fertilizer from the marine brown algae through enzymolysis is high, so that the overall cost of the compound fertilizer is increased. Therefore, how to reduce the manufacturing cost of the compound fertilizer and ensure the quality of the compound fertilizer becomes the key of the production of the seaweed compound fertilizer.
Disclosure of Invention
Aiming at the technical problem of overhigh cost of the seaweed compound fertilizer, the invention provides the method for preparing the seaweed compound fertilizer, which can obviously reduce the preparation cost of the seaweed base fertilizer and simultaneously can obviously improve the fertility of the seaweed compound fertilizer so as to achieve the technical aims of reducing the cost and improving the quality.
In order to achieve the technical purpose, the invention adopts the technical scheme that: a production method of a seaweed compound fertilizer comprises the following steps: adding the seaweed into the enzymolysis liquid for enzymolysis, taking the seaweed liquid after enzymolysis to compound with other fertilizers to prepare the seaweed compound fertilizer, and is characterized in that: the seaweed liquid compounded with other fertilizers is prepared by taking supernatant generated after seaweed enzymolysis and performing enzyme reduction treatment on the supernatant; the enzyme reduction treatment is to add ammonium sulfate with the weight of 0.1-0.15 part into the supernatant obtained after the enzymolysis of the seaweed, precipitate for 6-12h under the condition of the temperature of less than or equal to 20 ℃, then take out the supernatant as seaweed liquid compounded with other fertilizers, add dipotassium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with the weight of 2-3 times of the precipitate to dissolve the precipitate to form recovered enzyme liquid, and prepare enzymolysis liquid for the enzymolysis of the seaweed by utilizing the recovered enzyme liquid.
Further, the dipotassium hydrogen phosphate-potassium dihydrogen phosphate buffer solution has a concentration of 0.02M and a pH = 2-6.
Further, the enzymatic hydrolysate is cellulase, alginate lyase or a mixture of the cellulase and the alginate lyase, and the mixing ratio of the cellulase and the alginate lyase is as follows: and (3) quality of cellulase: alginate lyase =1:6, and the enzymatic activity of the enzymolysis liquid is more than or equal to 511U/mL.
Further, the preparation of the enzymatic hydrolysate for carrying out the enzymatic hydrolysis on the seaweed by utilizing the recovered enzyme solution is to add water and cellulase, alginate lyase or the mixture of the cellulase and the alginate lyase into the recovered enzyme solution until the enzyme activity is more than or equal to 511U/mL, wherein the mixing ratio of the cellulase to the alginate lyase is 1: 6.
Further, the step of adding the seaweed into the enzymolysis liquid for enzymolysis comprises the following steps: cutting 1 part of kelp into small pieces, adding 0.5 part of clear water, then adding 0.02 part of softener, adding 0.03-0.05 part of enzymolysis liquid, starting stirring, and carrying out enzymolysis at 40 ℃ for 16-18 h.
Further, the other fertilizers compounded by the seaweed liquid comprise 0.01 to 0.02 weight part of potassium sulfate, 0.02 to 0.03 weight part of boric acid and 0.01 to 0.02 weight part of sodium polyacrylate.
The invention has the beneficial effects that: the enzyme recovery method adopted by the invention reduces the waste of energy and enzyme, the enzyme extraction ratio is 15-25%, and the enzyme production cost is greatly reduced. The ammonium sulfate enzyme precipitation method adopted in the invention is suitable for fertilizer production technology, and the problem of ammonium sulfate recovery needs to be considered in the common production process of applying the enzyme precipitation method, so that the recovery difficulty is high, pollution and waste are easily caused, and the ammonium sulfate is a common fertilizer raw material and is suitable for various soils and crops.
Detailed Description
Example 1
1. Pulping 1 t of fresh kelp, adding 50% by mass of clear water, adding 35 Kg of alginate lyase enzyme liquid, wherein the enzyme activity of the enzyme liquid is 511U/mL, adding 20Kg of softener, starting stirring, carrying out enzymolysis for 17h, centrifuging the enzymolysis liquid, and removing kelp residues to obtain 1302Kg of supernatant.
3. 140 kg of ammonium sulfate was added to the supernatant and precipitated at 15 ℃ for 10 hours.
4. And centrifuging the enzyme precipitation liquid, pouring out supernatant liquid to obtain 1419.5Kg of supernatant liquid and 1.5Kg of precipitate, wherein the supernatant liquid is compounded with 21.3 Kg of potassium sulfate, 35.48 Kg of boric acid and 21.3 Kg of sodium polyacrylate to obtain the seaweed fertilizer.
5. 3.75 kg of 0.02M (pH2.0) dipotassium hydrogen phosphate-potassium dihydrogen phosphate buffer solution is added into the precipitate to be dissolved to obtain recovered enzyme solution, the recovered enzyme solution can be supplemented into the originally prepared enzymolysis solution with the enzyme activity value of 511U/mL, the enzyme activity value is tested to be 519U/mL, and the solution is reused for the enzymolysis of the kelp.
Through the steps, 14.28% of enzyme solution is successfully recovered, the value is based on the enzyme activity value of the recovered enzyme solution being more than 511U/mL, and the weight of the recovered enzyme solution accounts for the weight ratio of the originally used enzymolysis solution. The quality and the enzyme activity of the enzymolysis liquid are shown in the following table 1.
TABLE 1 recovery of enzyme solution in example 1
| Mass (kg) | Enzyme activity (U/mL) | Recovery ratio (%) | |
| Enzymolysis liquid | 35kg | 511 | |
| Recovering enzyme solution | 5.25kg | 519 | 15 |
Example 2
1. Pulping 1 t of fresh kelp, adding 50% by mass of clear water, adding 30 Kg by mass of brown cellulose enzyme solution, starting stirring, carrying out enzymolysis for 18h, centrifuging the enzymolysis solution, and removing kelp residues to obtain 1365Kg of supernatant fluid, wherein the enzyme activity of the enzyme solution is 516U/mL and 20K of softener.
3. 136.5 kg of ammonium sulfate was added to the supernatant, and the mixture was precipitated at 20 ℃ for 6 hours.
4. And centrifuging the enzyme precipitation liquid, and pouring out supernatant to obtain 1406Kg of supernatant and 2.5Kg of precipitate, wherein the supernatant is compounded with 27.3Kg of potassium sulfate, 27.3Kg of boric acid and 27.3Kg of sodium polyacrylate, namely the seaweed fertilizer.
5. And adding 5kg of 0.02M (pH6.0) dipotassium hydrogen phosphate-potassium dihydrogen phosphate buffer solution into the precipitate for dissolving to obtain recovered enzyme solution, supplementing the recovered enzyme solution into the originally prepared enzymolysis solution with the enzyme activity value of 516U/mL, testing the enzyme activity value to be 523U/mL, and reusing the enzyme solution for enzymolysis of the kelp.
Through the steps, 15.6% of enzyme solution is successfully recovered, the value is based on the enzyme activity value of the recovered enzyme solution being more than 511U/mL, and the weight of the recovered enzyme solution accounts for the weight ratio of the originally used enzymolysis solution. The quality and the enzyme activity of the enzymolysis liquid are shown in the following table 2.
TABLE 2 recovery of enzyme solution in example 2
| Mass (kg) | Enzyme activity (U/mL) | Recovery ratio (%) | |
| Initial addition of enzyme solution | 30 | 516 | |
| Recovering enzyme solution | 7.5 | 523 | 25 |
Example 3
1. Pulping 1 t of fresh kelp, adding 50% by mass of clear water, adding 40 Kg of enzymolysis liquid, wherein the enzymolysis liquid is prepared by mixing cellulase and alginate lyase, the ratio of the cellulase to the alginate lyase to the enzymolysis liquid is 1:6, the enzyme activity of the enzymolysis liquid is 525U/mL, and 20K softener, starting stirring, carrying out enzymolysis for 16 h, centrifuging the enzymolysis liquid, and removing kelp residues to obtain 1372Kg of supernatant.
3. 205.8 kg of ammonium sulfate was added to the supernatant, and the mixture was precipitated at 18 ℃ for 12 hours.
4. And centrifuging the enzyme precipitation liquid, pouring out the supernatant to obtain 1585Kg of supernatant and 3Kg of precipitate, wherein 23.7 Kg of potassium sulfate, 39.6Kg of boric acid and 23.7 Kg of sodium polyacrylate are compounded in the supernatant to obtain the seaweed fertilizer.
5. Adding 7.5 kg of 0.02M (pH4.0) dipotassium hydrogen phosphate-potassium dihydrogen phosphate buffer solution into the precipitate, dissolving to obtain recovered enzyme solution, supplementing the recovered enzyme solution into the initially prepared enzymolysis solution with the enzyme activity value of 525U/mL, testing the enzyme activity value of 531U/mL, and reusing the enzyme solution for enzymolysis of the kelp.
Through the steps, 15.6% of enzyme solution is successfully recovered, the value is based on the enzyme activity value of the recovered enzyme solution being more than 511U/mL, and the weight of the recovered enzyme solution accounts for the weight ratio of the originally used enzymolysis solution. The quality and the enzyme activity of the enzymatic hydrolysate are shown in the following table 3.
TABLE 3 enzyme solution recovery in example 3
| Mass (kg) | Enzyme activity (U/mL) | Recovery ratio (%) | |
| Initial addition of enzyme solution | 40 | 525 | |
| Recovering enzyme solution | 7.5 | 531 | 18.75 |
The experiment proves that: after the ammonium sulfate is adopted for enzyme reduction treatment, a large amount of bacterial enzymes which survive in the enzymolysis liquid after one time of enzymolysis can be recovered, and the use amount of the enzymes can be reduced by using the surviving enzymes to be put into the next time of enzymolysis, so that the enzymolysis cost of the kelp is reduced.
Fertilizer efficiency comparison experiment:
the seaweed compound fertilizer prepared in the example 2 is used as an experimental group, the supernatant obtained after the first enzymolysis in the example 2 is directly compounded with potassium sulfate, boric acid and sodium polyacrylate to prepare the seaweed compound fertilizer which is used as a control group, and the compounding ratio is consistent with that of the experimental group.
The test was carried out in Kaipu Bio Inc., Rong Cheng City. The experimental field and the control field are respectively square meters with the area of 25 square meters (the length and the width are 5 m), the wheat seeds are purchased in the farmer market of honor city, and the sowing mode adopts drilling. The same amount of seaweed fertilizer is applied to the experimental field and the control field, watering is carried out regularly, and other fertilizers are not applied. As the main difference between the seaweed fertilizers in the field and the control field is ammonium salt generated by ammonium sulfate during enzyme reduction, and the ammonium salt mainly promotes the development of nutritional organs such as wheat roots, stems, leaves and the like, the sampling at the jointing stage and the heading stage is selected to determine the growth condition.
Respectively sampling 50 plants at random in the jointing stage and the heading stage, measuring the fresh weight, the dry weight and the plant length of the plants, and recording data after averaging.
TABLE 4 influence of different fertilisations on the dry and wet weight and length of plants in the elongation and heading stages
As can be seen from the results in Table 4, the wheat growth conditions in the test field are better than those in the control field, the fresh weight and plant length differences are smaller, but the dry weight quality is obviously better than that in the comparative example, which indicates that the wheat has better thriving degree and is more plump in growth. Therefore, the seaweed liquid compounded by the ammonium sulfate enzyme reduction treatment can reduce the enzymolysis cost, and the fertility of the compounded seaweed compound fertilizer is further improved.
Claims (6)
1. A production method of a seaweed compound fertilizer comprises the following steps: adding the seaweed into the enzymolysis liquid for enzymolysis, taking the seaweed liquid after enzymolysis to compound with other fertilizers to prepare the seaweed compound fertilizer, and is characterized in that: the seaweed liquid compounded with other fertilizers is prepared by taking supernatant generated after seaweed enzymolysis and performing enzyme reduction treatment on the supernatant; the enzyme reduction treatment is to add ammonium sulfate with the weight of 0.1-0.15 part into the supernatant obtained after the enzymolysis of the seaweed, precipitate for 6-12h under the condition of the temperature of less than or equal to 20 ℃, then take out the supernatant as seaweed liquid compounded with other fertilizers, add dipotassium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with the weight of 2-3 times of the precipitate to dissolve the precipitate to form recovered enzyme liquid, and prepare enzymolysis liquid for the enzymolysis of the seaweed by utilizing the recovered enzyme liquid.
2. The method for producing a seaweed compound fertilizer according to claim 1, characterized in that: the concentration of the dipotassium hydrogen phosphate-potassium dihydrogen phosphate buffer solution is 0.02M, and the pH value is = 2-6.
3. The method for producing a seaweed compound fertilizer according to claim 1, characterized in that: the enzymolysis liquid is cellulase, algin lyase or the mixture of the cellulase and the algin lyase, and the mixing ratio of the cellulase and the algin lyase is as follows: and (3) quality of cellulase: alginate lyase =1:6, and the enzymatic activity of the enzymolysis liquid is more than or equal to 511U/mL.
4. The method for producing a seaweed compound fertilizer according to claim 1, characterized in that: the method for preparing the enzymatic hydrolysate for performing enzymolysis on the seaweed by utilizing the recovered enzyme solution is to add water, cellulase, alginate lyase or a mixture of the cellulase and the alginate lyase into the recovered enzyme solution until the enzyme activity is more than or equal to 511U/mL, wherein the mixing ratio of the cellulase to the alginate lyase is 1: 6.
5. The method for producing a seaweed compound fertilizer according to claim 1, characterized in that: the steps of adding the seaweed into the enzymolysis liquid for enzymolysis are as follows: cutting 1 part of kelp into small pieces, adding 0.5 part of clear water, then adding 0.02 part of softener, adding 0.03-0.05 part of enzymolysis liquid, starting stirring, and carrying out enzymolysis at 40 ℃ for 16-18 h.
6. The method for producing a seaweed compound fertilizer according to claim 1, characterized in that: the other fertilizers compounded by the seaweed liquid comprise 0.01 to 0.02 weight part of potassium sulfate, 0.02 to 0.03 weight part of boric acid and 0.01 to 0.02 weight part of sodium polyacrylate.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101319197A (en) * | 2007-06-05 | 2008-12-10 | 中国海洋大学 | Algin catenase prepared with Vibrio sp.JG07-007 |
| CN103145496A (en) * | 2013-02-27 | 2013-06-12 | 山东嘉丰海洋生物科技有限公司 | Method for preparing seaweed plant vegetative growth regulating agent by using biological enzymolysis method |
| US20170275213A1 (en) * | 2014-08-26 | 2017-09-28 | Weihai Shidai Marine Biotechnology Co., Ltd. | Composite microorganism enzyme, method for preparing plant nutrient solution by using composite microorganism enzyme, and fertilizer synergist |
| CN110256138A (en) * | 2019-04-17 | 2019-09-20 | 青岛明月蓝海生物科技有限公司 | A kind of clear liquid type water-soluble fertilizer and preparation method thereof |
| CN110590446A (en) * | 2019-09-25 | 2019-12-20 | 青岛佰蓝海藻有限公司 | Method for preparing liquid seaweed fertilizer by using kelp as raw material |
| CN111499431A (en) * | 2020-03-23 | 2020-08-07 | 青岛明月蓝海生物科技有限公司 | Preparation method of seaweed compound fertilizer |
-
2020
- 2020-12-22 CN CN202011523555.6A patent/CN112624842A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101319197A (en) * | 2007-06-05 | 2008-12-10 | 中国海洋大学 | Algin catenase prepared with Vibrio sp.JG07-007 |
| CN103145496A (en) * | 2013-02-27 | 2013-06-12 | 山东嘉丰海洋生物科技有限公司 | Method for preparing seaweed plant vegetative growth regulating agent by using biological enzymolysis method |
| US20170275213A1 (en) * | 2014-08-26 | 2017-09-28 | Weihai Shidai Marine Biotechnology Co., Ltd. | Composite microorganism enzyme, method for preparing plant nutrient solution by using composite microorganism enzyme, and fertilizer synergist |
| CN110256138A (en) * | 2019-04-17 | 2019-09-20 | 青岛明月蓝海生物科技有限公司 | A kind of clear liquid type water-soluble fertilizer and preparation method thereof |
| CN110590446A (en) * | 2019-09-25 | 2019-12-20 | 青岛佰蓝海藻有限公司 | Method for preparing liquid seaweed fertilizer by using kelp as raw material |
| CN111499431A (en) * | 2020-03-23 | 2020-08-07 | 青岛明月蓝海生物科技有限公司 | Preparation method of seaweed compound fertilizer |
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Application publication date: 20210409 |