CN116103269A - Complex enzyme and application method thereof in preparation of water-soluble fertilizer - Google Patents
Complex enzyme and application method thereof in preparation of water-soluble fertilizer Download PDFInfo
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- CN116103269A CN116103269A CN202211684713.5A CN202211684713A CN116103269A CN 116103269 A CN116103269 A CN 116103269A CN 202211684713 A CN202211684713 A CN 202211684713A CN 116103269 A CN116103269 A CN 116103269A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22002—Papain (3.4.22.2)
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a compound enzyme, which consists of cellulase, xylitol enzyme and papain according to the mass ratio of 2-4:1-5:0.5-0.7. The application method of the complex enzyme in the preparation of the water-soluble fertilizer comprises the following steps: (1) preparing soybean into soybean meal powder for standby; (2) The raw materials are mixed and stirred uniformly and then subjected to fermentation enzymolysis to prepare fermentation enzymolysis liquid; (3) Filtering the fermentation enzymolysis liquid prepared in the step (2) by using a filtering tank, and then placing the filtered fermentation enzymolysis liquid into a filter press for solid-liquid separation to prepare filtrate; (4) And (3) placing the filtrate obtained in the step (3) in an inactivating device for inactivating treatment to obtain the water-soluble fertilizer. The water-soluble fertilizer product produced by the invention contains rich active elements such as plant amino acid small peptide, polypeptide, oligosaccharide and the like and various chelate trace elements, no waste residue is produced in the preparation process, no emission is produced, the energy consumption is low, the production process is simple and convenient and easy to control, and the large-scale production is easy to form, thereby being beneficial to reducing the cost and enhancing the efficiency.
Description
[ field of technology ]
The invention belongs to the technical field of water-soluble fertilizers, and particularly relates to a compound enzyme and a use method thereof in the preparation of a water-soluble fertilizer.
[ background Art ]
The water-soluble fertilizer is a compound fertilizer containing nitrogen, phosphorus, potassium, calcium, magnesium, trace elements, amino acids, humic acid, alginic acid and the like, and can be completely dissolved in water. Strict standards are applied to the quality of raw materials and the treatment process. Research shows that the quality of fruits and vegetables can be improved by using the water-soluble fertilizer.
The water-soluble fertilizer has a great boosting effect on the development of modern agriculture in China, but the existing water-soluble fertilizer still has some technical problems, such as complex production process, long production period, easy generation of a large amount of byproducts to pollute the environment, low organic matter content, low free amino acid content, low product activity degree, inability of well improving the current state of soil hardening and the like. Therefore, how to develop a water-soluble fertilizer with short production period and high quality becomes a new research direction.
[ invention ]
The invention provides a compound enzyme and a use method thereof in the preparation of a water-soluble fertilizer, which are used for solving the problems of long production period, low product activity, poor soil improvement effect and the like of the water-soluble fertilizer prepared by the prior art.
In order to solve the technical problems, the invention adopts the following technical scheme:
a compound enzyme comprises cellulase, xylitol enzyme and papain at a mass ratio of 2-4:1-5:0.5-0.7.
Preferably, the microbial feed consists of cellulase, xylitol enzyme and papain according to the mass ratio of 3:4:0.6.
The invention also provides a use method of the compound enzyme in the production of the water-soluble fertilizer, which comprises the following steps:
(1) Preparing soybean into soybean meal powder for later use;
(2) Uniformly mixing and stirring soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium sources, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, zymophyte, compound enzyme, modifier and water, and then carrying out fermentation enzymolysis to obtain fermentation enzymolysis liquid;
(3) Filtering the fermentation enzymolysis liquid prepared in the step (2) by using a filtering tank, and then placing the filtered fermentation enzymolysis liquid into a filter press for solid-liquid separation to prepare filtrate;
(4) And (3) placing the filtrate obtained in the step (3) in an inactivating device for inactivating treatment to obtain the water-soluble fertilizer.
Preferably, the step (2) comprises the following raw materials by weight part, mixing and stirring uniformly: 60-70 parts of bean pulp powder, 10-15 parts of seaweed extract, 5-7 parts of organic nitrogen, phosphorus and potassium sources, 4-8 parts of mannitol, 2-5 parts of glucose, 4-8 parts of seaweed polysaccharide, 2-4 parts of polyglutamic acid, 0.04-0.08 part of salicylic acid, 0.3-0.5 part of zymocyte, 0.1-0.2 part of complex enzyme, 0.2-0.8 part of modifier and 120-200 parts of water.
Preferably, the organic nitrogen, phosphorus and potassium source is brown sugar.
Preferably, the modifier is prepared from polyacrylamide, hydroxyapatite and humic acid according to the mass ratio of 0.5-1:2-5:2-3.
More preferably, the modifier is prepared from polyacrylamide, hydroxyapatite and humic acid according to the mass ratio of 0.8:4:2.7.
Preferably, the zymophyte consists of saccharomycetes, lactobacillus and bacillus licheniformis according to the mass ratio of 4-8:0.3-0.6:1-2.
More preferably, the zymophyte consists of saccharomycetes, lactobacillus and bacillus licheniformis according to the mass ratio of 6:0.5:1.3.
Preferably, the conditions for carrying out the fermentation and enzymolysis in the step (2): fermenting and hydrolyzing at 25-32deg.C for 5-8 days.
The invention has the following beneficial effects:
(1) The complex enzyme adopted by the invention consists of cellulase, xylitol enzyme and papain, and a certain proportion is controlled, and the three enzymes are mutually matched, so that the enzymolysis of raw materials can be effectively accelerated, the completion can be realized within 5-8 days, and the production period is greatly shortened.
(2) In the modifier, the polyacrylamide can effectively improve the soil structure, improve the soil porosity and capillary porosity, increase the number of large soil aggregates and reduce the volume weight of the soil. The soil water infiltration and water content are promoted, the fertilizer utilization rate of the soil is increased, the effects of improving the soil fertility and increasing the yield are achieved, the erosion of the soil can be effectively prevented, meanwhile, the microorganisms in the soil are not influenced, and the soil planting efficiency is ensured; the hydroxyapatite contains a large amount of calcium ions, the structure of the hydroxyapatite contains holes and a large specific surface area, and the heavy metal in the soil and OH-thereof are combined to form a hydroxyl compound which provides more adsorption sites for heavy metal ions, so that the heavy metal ions in the soil can be adsorbed, and the pH value of the soil can be regulated; humic acid interacts with calcium ions in soil to form flocculent precipitated gel. The soil can be glued together to form a water-stable aggregate structure, and the permeability, the absorbability and the storage property of water and air can be maintained, so that the requirements of water, oxygen and air in the plant growth process are met, and complexes can be formed with iron and aluminum in the soil, so that the toxicity of acidic oxides to crops is reduced, and the buffer effect on alkaline substances is achieved.
(3) The hydroxyapatite disclosed by the invention can bear polyacrylamide due to the large specific surface area, so that the infiltration depth of the polyacrylamide to soil is increased, the existence of calcium ion components can increase the viscosity of a polyacrylamide aqueous solution, the infiltration rate of the polyacrylamide to the soil is increased, and the agglomeration effect of the polyacrylamide to the soil is improved; the polyacrylamide aqueous solution can fix heavy metals in soil, is favorable for the exchange adsorption of the hydroxyapatite on the heavy metal ions, and increases the removal of the hydroxyapatite on the heavy metal ions; humic acid can improve the porosity of soil, is favorable to improving the infiltration depth of the hydroxyapatite loaded polyacrylamide into the soil, increases the action effect, can increase the water retention performance of the soil, is favorable to the viscosity of the polyacrylamide aqueous solution, and further improves the infiltration rate of the polyacrylamide.
(4) The water-soluble fertilizer product produced by the invention contains rich active elements such as plant amino acid small peptide, polypeptide, oligosaccharide and the like and various chelate trace elements, no waste residue is produced in the preparation process, no emission is produced, the energy consumption is low, the production process is simple and convenient and easy to control, and the large-scale production is easy to form, thereby being beneficial to reducing the cost and enhancing the efficiency.
(5) The water-soluble fertilizer prepared by the invention has excellent technical indexes, wherein the organic matter content is more than or equal to 200g/L; the content of free amino acid is more than or equal to 80g/L; the nitrogen, phosphorus and potassium are more than or equal to 100g/L; aluminum, zinc, boron, iron, calcium, magnesium >30g/L; the water insoluble matter is less than 20g/L, and can meet the growth requirement of crops.
[ detailed description ] of the invention
For a better understanding of the present invention, reference is made to the following examples, which are included within the scope of the present invention, but are not intended to limit the scope of the present invention.
A water-soluble fertilizer is prepared from the following raw materials in parts by weight: 60-70 parts of bean pulp powder, 10-15 parts of seaweed extract, 5-7 parts of organic nitrogen, phosphorus and potassium sources, 4-8 parts of mannitol, 2-5 parts of glucose, 4-8 parts of seaweed polysaccharide, 2-4 parts of polyglutamic acid, 0.04-0.08 part of salicylic acid, 0.3-0.5 part of zymocyte, 0.1-0.2 part of complex enzyme, 0.2-0.8 part of modifier and 120-200 parts of water.
The organic nitrogen, phosphorus and potassium source is brown sugar.
The complex enzyme consists of cellulase, xylitol enzyme and papain according to the mass ratio of 2-4:1-5:0.5-0.7.
The modifier is prepared from polyacrylamide, hydroxyapatite and humic acid according to the mass ratio of 0.5-1:2-5:2-3.
The zymophyte consists of saccharomycetes, lactobacillus and bacillus licheniformis according to the mass ratio of 4-8:0.3-0.6:1-2.
The application method of the complex enzyme in the preparation of the water-soluble fertilizer comprises the following steps:
(1) Preparing soybean into soybean meal powder for later use;
(2) Uniformly mixing and stirring soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium sources, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, zymophyte, compound enzyme, modifier and water, and then carrying out fermentation enzymolysis for 5-8 days at 25-32 ℃ to obtain fermentation enzymolysis liquid;
(3) Filtering the fermentation enzymolysis liquid prepared in the step (2) by using a filtering tank, and then placing the filtered fermentation enzymolysis liquid into a filter press for solid-liquid separation to prepare filtrate;
(4) And (3) placing the filtrate obtained in the step (3) in an inactivating device for inactivating treatment to obtain the water-soluble fertilizer.
The following is a description of more specific embodiments.
Example 1
A water-soluble fertilizer is prepared from the following raw materials in parts by weight: 70 parts of bean pulp powder, 10 parts of seaweed extract, 6 parts of organic nitrogen, phosphorus and potassium sources, 8 parts of mannitol, 3 parts of glucose, 5 parts of seaweed polysaccharide, 4 parts of polyglutamic acid, 0.05 part of salicylic acid, 0.4 part of zymocyte, 0.2 part of complex enzyme, 0.6 part of modifier and 180 parts of water.
The organic nitrogen, phosphorus and potassium source is brown sugar.
The compound enzyme consists of cellulase, xylitol enzyme and papain according to the mass ratio of 4:2:0.5.
The modifier is prepared from polyacrylamide, hydroxyapatite and humic acid according to the mass ratio of 0.8:3:2.
The zymophyte consists of saccharomycetes, lactobacillus and bacillus licheniformis according to the mass ratio of 5:0.3:1.
The application method of the complex enzyme in the preparation of the water-soluble fertilizer comprises the following steps:
(1) Preparing soybean into soybean meal powder for later use;
(2) Uniformly mixing and stirring soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium sources, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, zymophyte, compound enzyme, modifier and water, and then carrying out fermentation and enzymolysis for 8 days at the temperature of 26 ℃ to obtain fermentation enzymolysis liquid;
(3) Filtering the fermentation enzymolysis liquid prepared in the step (2) by using a filtering tank, and then placing the filtered fermentation enzymolysis liquid into a filter press for solid-liquid separation to prepare filtrate;
(4) And (3) placing the filtrate obtained in the step (3) in an inactivating device for inactivating treatment to obtain the water-soluble fertilizer.
Example 2
A water-soluble fertilizer is prepared from the following raw materials in parts by weight: 60 parts of bean pulp powder, 12 parts of seaweed extract, 7 parts of organic nitrogen, phosphorus and potassium source, 4 parts of mannitol, 2 parts of glucose, 4 parts of seaweed polysaccharide, 2 parts of polyglutamic acid, 0.04 part of salicylic acid, 0.5 part of zymocyte, 0.1 part of complex enzyme, 0.2 part of modifier and 150 parts of water.
The organic nitrogen, phosphorus and potassium source is brown sugar.
The compound enzyme consists of cellulase, xylitol enzyme and papain according to the mass ratio of 2:2:0.7.
The modifier is prepared from polyacrylamide, hydroxyapatite and humic acid according to the mass ratio of 0.5:5:3.
The zymophyte consists of saccharomycetes, lactobacillus and bacillus licheniformis according to the mass ratio of 4:0.5:2.
The application method of the complex enzyme in the preparation of the water-soluble fertilizer comprises the following steps:
(1) Preparing soybean into soybean meal powder for later use;
(2) Uniformly mixing and stirring soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium sources, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, zymophyte, compound enzyme, modifier and water, and then carrying out fermentation and enzymolysis for 8 days at the temperature of 25 ℃ to obtain fermentation enzymolysis liquid;
(3) Filtering the fermentation enzymolysis liquid prepared in the step (2) by using a filtering tank, and then placing the filtered fermentation enzymolysis liquid into a filter press for solid-liquid separation to prepare filtrate;
(4) And (3) placing the filtrate obtained in the step (3) in an inactivating device for inactivating treatment to obtain the water-soluble fertilizer.
Example 3
A water-soluble fertilizer is prepared from the following raw materials in parts by weight: 60 parts of bean pulp powder, 15 parts of seaweed extract, 5 parts of organic nitrogen, phosphorus and potassium source, 6 parts of mannitol, 5 parts of glucose, 6 parts of seaweed polysaccharide, 2 parts of polyglutamic acid, 0.08 part of salicylic acid, 0.5 part of zymocyte, 0.1 part of complex enzyme, 0.4 part of modifier and 200 parts of water.
The organic nitrogen, phosphorus and potassium source is brown sugar.
The compound enzyme consists of cellulase, xylitol enzyme and papain according to the mass ratio of 3:4:0.6.
The modifier is prepared from polyacrylamide, hydroxyapatite and humic acid according to the mass ratio of 0.8:4:2.7.
The zymophyte consists of saccharomycetes, lactobacillus and bacillus licheniformis according to the mass ratio of 6:0.5:1.3.
The application method of the complex enzyme in the preparation of the water-soluble fertilizer comprises the following steps:
(1) Preparing soybean into soybean meal powder for later use;
(2) Uniformly mixing and stirring soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium sources, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, zymophyte, compound enzyme, modifier and water, and then carrying out fermentation and enzymolysis for 5 days at the temperature of 32 ℃ to obtain fermentation enzymolysis liquid;
(3) Filtering the fermentation enzymolysis liquid prepared in the step (2) by using a filtering tank, and then placing the filtered fermentation enzymolysis liquid into a filter press for solid-liquid separation to prepare filtrate;
(4) And (3) placing the filtrate obtained in the step (3) in an inactivating device for inactivating treatment to obtain the water-soluble fertilizer.
Example 4
A water-soluble fertilizer is prepared from the following raw materials in parts by weight: 65 parts of bean pulp powder, 10 parts of seaweed extract, 5 parts of organic nitrogen, phosphorus and potassium source, 4 parts of mannitol, 4 parts of glucose, 4 parts of seaweed polysaccharide, 4 parts of polyglutamic acid, 0.06 part of salicylic acid, 0.3 part of zymocyte, 0.2 part of complex enzyme, 0.8 part of modifier and 140 parts of water.
The organic nitrogen, phosphorus and potassium source is brown sugar.
The compound enzyme consists of cellulase, xylitol enzyme and papain according to the mass ratio of 3:4:0.7.
The modifier is prepared from polyacrylamide, hydroxyapatite and humic acid according to the mass ratio of 1:4:3.
The zymophyte consists of saccharomycetes, lactobacillus and bacillus licheniformis according to the mass ratio of 8:0.6:2.
The application method of the complex enzyme in the preparation of the water-soluble fertilizer comprises the following steps:
(1) Preparing soybean into soybean meal powder for later use;
(2) Uniformly mixing and stirring soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium sources, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, zymophyte, compound enzyme, modifier and water, and then carrying out fermentation and enzymolysis for 6 days at the temperature of 28 ℃ to obtain fermentation enzymolysis liquid;
(3) Filtering the fermentation enzymolysis liquid prepared in the step (2) by using a filtering tank, and then placing the filtered fermentation enzymolysis liquid into a filter press for solid-liquid separation to prepare filtrate;
(4) And (3) placing the filtrate obtained in the step (3) in an inactivating device for inactivating treatment to obtain the water-soluble fertilizer.
Example 5
A water-soluble fertilizer is prepared from the following raw materials in parts by weight: 70 parts of bean pulp powder, 12 parts of seaweed extract, 7 parts of organic nitrogen, phosphorus and potassium source, 8 parts of mannitol, 2 parts of glucose, 8 parts of seaweed polysaccharide, 4 parts of polyglutamic acid, 0.05 part of salicylic acid, 0.3 part of zymocyte, 0.2 part of complex enzyme, 0.5 part of modifier and 120 parts of water.
The organic nitrogen, phosphorus and potassium source is brown sugar.
The compound enzyme consists of cellulase, xylitol enzyme and papain according to the mass ratio of 2:5:0.6.
The modifier is prepared from polyacrylamide, hydroxyapatite and humic acid according to the mass ratio of 0.5:2:3.
The zymophyte consists of saccharomycetes, lactobacillus and bacillus licheniformis according to the mass ratio of 4:0.4:1.
The application method of the complex enzyme in the preparation of the water-soluble fertilizer comprises the following steps:
(1) Preparing soybean into soybean meal powder for later use;
(2) Uniformly mixing and stirring soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium sources, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, zymophyte, compound enzyme, modifier and water, and then carrying out fermentation and enzymolysis for 6 days at the temperature of 30 ℃ to obtain fermentation enzymolysis liquid;
(3) Filtering the fermentation enzymolysis liquid prepared in the step (2) by using a filtering tank, and then placing the filtered fermentation enzymolysis liquid into a filter press for solid-liquid separation to prepare filtrate;
(4) And (3) placing the filtrate obtained in the step (3) in an inactivating device for inactivating treatment to obtain the water-soluble fertilizer.
Comparative example 1
Substantially the same as in example 3, except that the water-soluble fertilizer was prepared by the raw material lacking the modifier, the difference was made up with water.
Comparative example 2
Substantially the same as in example 3, except that polyacrylamide was absent from the raw material for preparing the water-soluble fertilizer, the difference was made up with water.
Comparative example 3
Substantially the same as in example 3, except that hydroxyapatite was absent from the raw material for preparing the water-soluble fertilizer, the difference was made up with water.
Comparative example 4
Substantially the same as in example 3, except that humic acid was absent from the raw material for producing a water-soluble fertilizer, the difference was made up with water.
Comparative example 5
Substantially the same as in example 3, except that the raw material for preparing the water-soluble fertilizer lacks the complex enzyme, the difference is made up with water.
Comparative example 6
Substantially the same as in example 3, except that xylitol enzyme was absent from the raw material for producing the water-soluble fertilizer, the difference was made up with water.
Comparative example 7
Substantially the same as in example 3, except that the raw material for preparing a water-soluble fertilizer lacks cellulase, the difference is made up with water.
Comparative example 8
Substantially the same as in example 3, except that papain was absent from the raw materials for preparing the water-soluble fertilizer, the difference was made up with water.
Application of water-soluble fertilizer
Selecting an experimental base in a three-pond town Su Cun of Xingning area in Nanning, selecting 13 lands with basically consistent conditions, planting tomatoes of the same variety, and spraying from the seedling stage of the tomatoes, wherein the water-soluble fertilizer prepared in each spraying of examples 1-5 and comparative examples 1-8 is used by the following steps: the fertilizer is diluted by 200 times with water before application, the dosage per mu is 200mL, the fertilizer is respectively fertilized for 1 time every 7 days, the total application is three times, the conditions of other planting processes are basically consistent, the yield per mu is measured after tomatoes are ripe, and the results are shown in the following table.
| Group of | Mu yield (kg) |
| Example 1 | 4790 |
| Example 2 | 4843 |
| Example 3 | 4864 |
| Example 4 | 4793 |
| Example 5 | 4826 |
| Comparative example 1 | 3675 |
| Comparative example 2 | 4712 |
| Comparative example 3 | 4567 |
| Comparative example 4 | 4421 |
| Comparative example 5 | 4071 |
| Comparative example 6 | 4608 |
| Comparative example 7 | 4552 |
| Comparative example 8 | 4734 |
As can be seen from the table, the water-soluble fertilizer provided by the invention has the advantages that the acre yield of the tomatoes in the examples 1-5 reaches more than 4790kg, the acre yield of the example 3 reaches 4864kg, the yield of crops is greatly improved, and the economic benefit is high.
As can be seen from comparative examples 1-4, the polyacrylamide, the hydroxyapatite and the humic acid in the modifier provided by the invention have synergistic reaction, so that the yield of tomatoes per mu can be increased, and the economic benefit is further improved.
As can be seen from comparative examples 5-8, the xylitol enzyme, the cellulase and the papain of the invention also produce synergistic reaction, so that the acre yield of tomatoes is further improved, and the boosting economy is improved.
Claims (10)
1. A compound enzyme is characterized by comprising cellulase, xylitol enzyme and papain according to the mass ratio of 2-4:1-5:0.5-0.7.
2. The complex enzyme according to claim 1, which comprises cellulase, xylitol enzyme and papain in a mass ratio of 3:4:0.6.
3. A method of using the complex enzyme according to claim 1 or 2 in the manufacture of water-soluble fertilizers, comprising the steps of:
(1) Preparing soybean into soybean meal powder for later use;
(2) Uniformly mixing and stirring soybean meal powder, seaweed extract, organic nitrogen, phosphorus and potassium sources, mannitol, glucose, seaweed polysaccharide, polyglutamic acid, salicylic acid, zymophyte, compound enzyme, modifier and water, and then carrying out fermentation enzymolysis to obtain fermentation enzymolysis liquid;
(3) Filtering the fermentation enzymolysis liquid prepared in the step (2) by using a filtering tank, and then placing the filtered fermentation enzymolysis liquid into a filter press for solid-liquid separation to prepare filtrate;
(4) And (3) placing the filtrate obtained in the step (3) in an inactivating device for inactivating treatment to obtain the water-soluble fertilizer.
4. The method for using the compound enzyme in the production of the water-soluble fertilizer according to claim 3, wherein the step (2) comprises the following raw materials by weight part, mixing and stirring uniformly: 60-70 parts of bean pulp powder, 10-15 parts of seaweed extract, 5-7 parts of organic nitrogen, phosphorus and potassium sources, 4-8 parts of mannitol, 2-5 parts of glucose, 4-8 parts of seaweed polysaccharide, 2-4 parts of polyglutamic acid, 0.04-0.08 part of salicylic acid, 0.3-0.5 part of zymocyte, 0.1-0.2 part of complex enzyme, 0.2-0.8 part of modifier and 120-200 parts of water.
5. The method for using the complex enzyme in the production of water-soluble fertilizer according to claim 3 or 4, wherein the organic nitrogen-phosphorus-potassium source is brown sugar.
6. The method for using the compound enzyme in the production of the water-soluble fertilizer according to claim 3 or 4, wherein the modifier is prepared from polyacrylamide, hydroxyapatite and humic acid according to the mass ratio of 0.5-1:2-5:2-3.
7. The method for using the compound enzyme in the production of the water-soluble fertilizer according to claim 6, wherein the modifier is prepared from polyacrylamide, hydroxyapatite and humic acid according to the mass ratio of 0.8:4:2.7.
8. The method for using the compound enzyme in the production of the water-soluble fertilizer according to claim 3 or 4, wherein the zymophyte consists of saccharomycetes, lactobacillus and bacillus licheniformis according to the mass ratio of 4-8:0.3-0.6:1-2.
9. The method for using the compound enzyme in the production of the water-soluble fertilizer according to claim 8, wherein the zymophyte consists of saccharomycetes, lactobacillus and bacillus licheniformis according to the mass ratio of 6:0.5:1.3.
10. The method of using the complex enzyme in the production of water-soluble fertilizer according to claim 3, wherein the conditions for fermentation and enzymolysis in the step (2) are as follows: fermenting and hydrolyzing at 25-32deg.C for 5-8 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211684713.5A CN116103269A (en) | 2022-12-27 | 2022-12-27 | Complex enzyme and application method thereof in preparation of water-soluble fertilizer |
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| CN117362084A (en) * | 2023-10-27 | 2024-01-09 | 中山市承铭农业技术开发有限公司 | Amino acid water-soluble fertilizer prepared from soybeans and production process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117362084A (en) * | 2023-10-27 | 2024-01-09 | 中山市承铭农业技术开发有限公司 | Amino acid water-soluble fertilizer prepared from soybeans and production process |
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