CN114525316A - Seaweed liquid and preparation method of seaweed fertilizer thereof - Google Patents
Seaweed liquid and preparation method of seaweed fertilizer thereof Download PDFInfo
- Publication number
- CN114525316A CN114525316A CN202210219470.1A CN202210219470A CN114525316A CN 114525316 A CN114525316 A CN 114525316A CN 202210219470 A CN202210219470 A CN 202210219470A CN 114525316 A CN114525316 A CN 114525316A
- Authority
- CN
- China
- Prior art keywords
- seaweed
- enzyme
- fertilizer
- enzymolysis
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to a seaweed liquid and its preparation method of seaweed fertilizer, the preparation method of seaweed extracted solution is, add seaweed into acetic acid sodium acetate buffer solution, after the enzymolysis of mixed enzyme, filter, the resulting filtrate is seaweed liquid, characterized by, the said mixed enzyme includes glucoamylase, cellulase and bovine serum albumin at least; the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 19-39: 60-80: 0.01-0.5; the mass ratio of the saccharifying enzyme to the cellulase to the bovine serum albumin in the mixed enzyme is 1-2: 5-15; the pH value of the sodium acetate buffer solution is 4.4-6.0. The method can solve the problems that saccharification enzyme and cellulase are different in enzymolysis conditions, step-by-step extraction is needed to ensure the enzyme activity, and the extraction efficiency is low in the preparation of algal polysaccharides through enzymolysis of seaweed.
Description
Technical Field
The invention relates to a preparation method of seaweed liquid and a seaweed fertilizer thereof.
Background
At present, algal polysaccharide is extracted from algal liquid by enzyme, generally, in order to maximize extraction, cellulase and glucoamylase are combined and sequentially extracted step by step, firstly, cellulase dissolves cell walls of algae, dissolves out intracellular solutes and hydrolyzes beta-1, 4 glycosidic bonds; and then saccharifying enzyme is adopted to hydrolyze alpha-1, 4-and alpha-1, 6-glycosidic bonds so as to improve the content of algal polysaccharides in the extracting solution, because the enzyme activity conditions of cellulase and saccharifying enzyme are different, and the extraction of saccharifying enzyme is carried out by dissolving out the seaweed cell inner solute by virtue of the enzymolysis of cellulase, if the cellulase and saccharifying enzyme are simultaneously extracted, the enzyme activity is greatly reduced along with the extension of the extraction time, a large amount of enzyme needs to be added to ensure the enzyme activity, and the problem of incomplete extraction also exists, so that the production cost of an enterprise is improved, and the problems of long extraction time and low production efficiency exist due to the fact that the extraction is carried out step by step. For example, the invention patent of our company, patent No. CN112538511A, is an extraction method of seaweed liquid, that is, a process of sequentially performing cellulase extraction and glucoamylase extraction step by step is adopted, although the extraction is relatively complete, the problems of long extraction time and low production efficiency exist.
At present, the method has no method for improving the extraction efficiency of algal polysaccharide without increasing the addition of enzyme and influencing the extraction rate of algal polysaccharide. The prepared seaweed liquid should not affect the fertilizer efficiency while changing the extraction method.
Disclosure of Invention
The invention provides a preparation method of seaweed liquid and a seaweed fertilizer thereof, which solves the technical problems that 1) the addition of enzyme is not increased, the extraction efficiency is improved, and the extraction is complete; 2) the prepared seaweed liquid should not affect the fertilizer efficiency while changing the extraction method.
In order to solve the technical problems, the invention adopts the following technical scheme:
a method for preparing Sargassum liquid comprises adding Sargassum and mixed enzyme into sodium acetate buffer solution, performing enzymolysis with mixed enzyme, and filtering to obtain filtrate, which is Sargassum liquid, wherein the mixed enzyme at least comprises diastase, cellulase and bovine serum albumin; the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 19-39: 60-80: 0.07-0.5; the mass ratio of the saccharifying enzyme to the cellulase to the bovine serum albumin in the mixed enzyme is 1-2: 5-15; the pH value of the sodium acetate buffer solution is 4.4-6.0; the seaweed is one or more of Porphyra haitanensis, herba Zosterae marinae, Eucheuma Gelatinosum and Sargassum.
The mixed enzyme enzymolysis condition is that the temperature is 37-50 ℃ and the time is 6-36 h.
After the mixed enzyme is subjected to enzymolysis, enzyme deactivation is also included, protease is added after the enzyme deactivation, and the protease enzymolysis is carried out; the mass ratio of the protease addition amount to the mixed enzyme addition amount is 1: 10-20; the enzymolysis condition of the protease is that the enzymolysis temperature is 26-35 ℃, and the enzymolysis time is 6-48 h;
the enzyme deactivation condition is that the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min.
After the enzymolysis of the protease, enzyme deactivation is also included; the enzyme deactivation and enzyme deactivation conditions are that the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min.
The protease enzymolysis also comprises adding one of sodium hydroxide, sodium carbonate, potassium carbonate or potassium hydroxide before enzymolysis, and adjusting pH to 7.0.
And the protease enzymolysis also comprises introducing nitrogen into the seaweed liquid before enzymolysis until the oxygen content in the seaweed liquid is lower than 0.2 mg/L.
And the protease enzymolysis also comprises introducing nitrogen into the seaweed liquid before enzymolysis until the oxygen content in the seaweed liquid is lower than 0.2 mg/L.
The preparation method of the seaweed fertilizer from the seaweed liquid comprises the following steps,
drying the seaweed liquid to obtain seaweed powder; the drying temperature is not higher than 85 ℃;
adding the seaweed liquid or seaweed powder into the fertilizer or the fertilizer raw material to obtain the seaweed fertilizer;
the fertilizer is one of a water-soluble fertilizer, a compound fertilizer, an organic-inorganic fertilizer, a biological organic fertilizer, a simple substance fertilizer and an organic fertilizer;
the fertilizer raw materials are one or more of potassium chloride, potassium sulfate, urea, monoammonium phosphate, diammonium phosphate, ammonium sulfate, ammonium chloride, monopotassium phosphate, bentonite, zinc sulfate, ammonium calcium nitrate and potassium nitrate.
The mass ratio of the seaweed liquid to the fertilizer or the fertilizer raw materials is 3-30: 70-99;
the mass ratio of the seaweed powder to the fertilizer or the fertilizer raw materials is 1-10: 90-99.
The invention has the following beneficial technical effects:
1. this application is through adding the marine alga in the acetic acid sodium acetate buffer to add bovine serum albumin, can improve algal polysaccharide's extraction efficiency, and can make algal polysaccharide draw completely.
2. Experiments in the application find that the content of free amino acid in the protease enzymolysis liquid can be improved by adjusting the pH to 7 before the protease enzymolysis.
3. According to the method, the extraction method is changed, and the prepared seaweed liquid does not affect the fertilizer efficiency.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1
A preparation method of seaweed liquid comprises adding seaweed into sodium acetate buffer solution, performing enzymolysis with mixed enzyme, inactivating enzyme, and filtering to obtain filtrate, wherein the mixed enzyme is a composition of saccharifying enzyme, cellulase and bovine serum albumin at a mass ratio of 1:1: 15;
the pH of the sodium acetate buffer solution is 4.8;
the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 30:68.1: 1.7; the seaweed is Porphyra haitanensis.
The enzymolysis condition of the mixed enzyme is 40 ℃; the enzymolysis time is 30 h.
The enzyme deactivation and enzyme deactivation conditions are that the temperature is 100 ℃ and the time is 2 min.
Wherein the activity of the saccharifying enzyme is 50000U/g, and the activity of the cellulase is 100000U/g; the above enzymes were purchased from Novoxin (China) Biotechnology Ltd.
The preparation method for preparing the seaweed fertilizer from the seaweed liquid comprises the following steps:
adding the seaweed liquid into a liquid water-soluble fertilizer to obtain a liquid seaweed fertilizer; the mass ratio of the seaweed liquid to the liquid water-soluble fertilizer is 10: 90;
the water-soluble fertilizer is a humic acid-containing water-soluble fertilizer and consists of urea, monopotassium phosphate, potassium humate and water according to a mass ratio of 30:10:5: 65.
Example 2
A preparation method of seaweed liquid comprises the steps of adding seaweed into sodium acetate buffer solution, carrying out enzymolysis through mixed enzyme, carrying out enzyme deactivation to obtain saccharification enzymolysis liquid, adding papain into the saccharification enzymolysis liquid, carrying out enzymolysis, carrying out enzyme deactivation, and filtering to obtain filtrate, namely the seaweed liquid, wherein the mixed enzyme is a composition of the glucoamylase, cellulase and bovine serum albumin according to the mass ratio of 1:1: 15;
the pH of the sodium acetate buffer solution is 4.8;
the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 30:68.1: 1.7; the seaweed is Porphyra haitanensis.
The enzymolysis condition of the mixed enzyme is 40 ℃; the enzymolysis time is 30 h.
The mass ratio of the protease addition amount to the mixed enzyme addition amount is 1: 17; the enzymolysis condition of the protease is that the enzymolysis temperature is 32 ℃ and the enzymolysis time is 24 hours;
the enzyme deactivation and enzyme deactivation conditions are that the temperature is 100 ℃ and the time is 2 min.
Wherein the activity of the saccharifying enzyme is 50000U/g, the activity of the cellulase is 100000U/g, the activity of the papain is 50000U/g, and the saccharifying enzyme is purchased from Novoxin (China) biotechnology limited.
The preparation method for preparing the seaweed fertilizer from the seaweed liquid comprises the following steps:
adding the seaweed liquid into a liquid water-soluble fertilizer to obtain a liquid seaweed fertilizer; the mass ratio of the seaweed liquid to the liquid water-soluble fertilizer is 10: 90.
The liquid water-soluble fertilizer is a humic acid-containing liquid water-soluble fertilizer and consists of urea, monopotassium phosphate, potassium humate and water according to a mass ratio of 30:10:5: 65.
Example 3
A preparation method of seaweed liquid comprises the steps of adding seaweed into a sodium acetate buffer solution, carrying out enzymolysis through mixed enzyme, carrying out enzyme deactivation to obtain saccharification enzymolysis liquid, adding a 5% sodium hydroxide aqueous solution into the saccharification enzymolysis liquid, adjusting the pH value to 7.0, adding papain, carrying out enzymolysis, carrying out enzyme deactivation, and filtering to obtain a filtrate, namely the seaweed liquid, wherein the mixed enzyme is a composition of the saccharifying enzyme, the cellulase and bovine serum albumin according to the mass ratio of 1:1: 15;
the pH of the sodium acetate buffer solution is 4.8;
the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 30:68.1: 1.7; the seaweed is Porphyra haitanensis.
The enzymolysis condition of the mixed enzyme is 40 ℃; the enzymolysis time is 30 h.
The mass ratio of the protease addition amount to the mixed enzyme addition amount is 1: 17; the enzymolysis condition of the protease is that the enzymolysis temperature is 32 ℃ and the enzymolysis time is 24 hours;
the enzyme deactivation and enzyme deactivation conditions are that the temperature is 100 ℃ and the time is 2 min.
Wherein the activity of the saccharifying enzyme is 50000U/g, the activity of the cellulase is 100000U/g, the activity of the papain is 50000U/g, and the saccharifying enzyme is purchased from Novoxin (China) biotechnology limited.
The preparation method for preparing the seaweed fertilizer from the seaweed liquid comprises the following steps:
adding the seaweed liquid into a liquid water-soluble fertilizer to obtain a liquid seaweed fertilizer; the mass ratio of the seaweed liquid to the liquid water-soluble fertilizer is 10: 90.
The liquid water-soluble fertilizer is a humic acid-containing liquid water-soluble fertilizer and consists of urea, monopotassium phosphate, potassium humate and water according to a mass ratio of 30:10:5: 65.
Example 4
A preparation method of seaweed liquid comprises adding seaweed into sodium acetate buffer solution, performing enzymolysis with mixed enzyme, inactivating enzyme, and filtering to obtain filtrate, wherein the mixed enzyme is a composition of saccharifying enzyme, cellulase and bovine serum albumin at a mass ratio of 1:1: 12;
the pH of the sodium acetate buffer solution is 5.2;
the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 25:73.5: 1.5; the seaweed is kelp.
The enzymolysis condition of the mixed enzyme is 47 ℃; the enzymolysis time is 32 h.
The enzyme deactivation and enzyme deactivation conditions are that the temperature is 100 ℃ and the time is 2 min.
Wherein the activity of saccharifying enzyme is 50000U/g, the activity of cellulase is 100000U/g, and the enzyme is purchased from Novoxin (China) Biotechnology Co., Ltd.
The preparation method for preparing the seaweed fertilizer from the seaweed liquid comprises the following steps:
drying the seaweed solution at 60 deg.C until the water content is 1.8% to obtain seaweed powder;
adding seaweed powder into a water-soluble fertilizer to obtain a seaweed fertilizer; the mass ratio of the seaweed powder to the water-soluble fertilizer is 5: 95;
the water-soluble fertilizer is a macroelement water-soluble fertilizer and consists of urea, monopotassium phosphate and potassium sulfate according to the mass ratio of 50:20: 30.
Example 5
A preparation method of seaweed liquid comprises the steps of adding seaweed into sodium acetate buffer solution, carrying out enzymolysis through mixed enzyme, carrying out enzyme deactivation to obtain saccharification enzymolysis liquid, adding flavourzyme into the saccharification enzymolysis liquid, carrying out enzymolysis, carrying out enzyme deactivation, and filtering to obtain filtrate, namely the seaweed liquid, wherein the mixed enzyme is a composition of the saccharifying enzyme, the cellulase and bovine serum albumin according to the mass ratio of 1:1: 12;
the pH of the sodium acetate buffer solution is 5.2;
the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 25:73.5: 1.5; the seaweed is kelp.
The enzymolysis condition of the mixed enzyme is 47 ℃; the enzymolysis time is 32 h.
The mass ratio of the protease addition amount to the mixed enzyme addition amount is 1: 6; the enzymolysis condition of the protease is that the enzymolysis temperature is 32 ℃ and the enzymolysis time is 24 hours;
the enzyme deactivation and enzyme deactivation conditions are that the temperature is 100 ℃ and the time is 2 min.
Wherein the activity of saccharifying enzyme is 50000U/g, the activity of cellulase is 100000U/g, the activity of flavourzyme is 18400U/g, and the enzymes are purchased from Novoxin (China) biotechnology limited company.
The preparation method for preparing the seaweed fertilizer from the seaweed liquid comprises the following steps:
drying the seaweed solution at 60 deg.C until the water content is 1.8% to obtain seaweed powder;
adding seaweed powder into a water-soluble fertilizer to obtain a seaweed fertilizer; the mass ratio of the seaweed powder to the water-soluble fertilizer is 5: 95;
the water-soluble fertilizer is a macroelement water-soluble fertilizer and consists of urea, monopotassium phosphate and potassium sulfate according to the mass ratio of 50:20: 30.
Example 6
A preparation method of seaweed liquid comprises the steps of adding seaweed into sodium acetate buffer solution, carrying out enzymolysis through mixed enzyme, carrying out enzyme deactivation to obtain saccharification enzymolysis liquid, adding sodium carbonate into the saccharification enzymolysis liquid to adjust the pH value to 7.0, adding flavourzyme, carrying out enzymolysis, carrying out enzyme deactivation, and filtering to obtain filtrate, namely the seaweed liquid, wherein the mixed enzyme is a composition of the saccharifying enzyme, cellulase and bovine serum albumin according to the mass ratio of 1:1: 12;
the pH of the sodium acetate buffer solution is 5.2;
the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 25:73.5: 1.5; the seaweed is kelp.
The enzymolysis condition of the mixed enzyme is 47 ℃; the enzymolysis time is 32 h.
The mass ratio of the protease addition amount to the mixed enzyme addition amount is 1: 6; the enzymolysis condition of the protease is that the enzymolysis temperature is 32 ℃ and the enzymolysis time is 24 h.
The enzyme deactivation and enzyme deactivation conditions are that the temperature is 100 ℃ and the time is 2 min.
Wherein the activity of saccharifying enzyme is 50000U/g, the activity of cellulase is 100000U/g, the activity of flavourzyme is 18400U/g, and the enzymes are purchased from Novoxin (China) biotechnology limited company.
The preparation method for preparing the seaweed fertilizer from the seaweed liquid comprises the following steps:
drying the seaweed solution at 60 deg.C until the water content is 1.8% to obtain seaweed powder;
adding seaweed powder into a water-soluble fertilizer to obtain a seaweed fertilizer; the mass ratio of the seaweed powder to the water-soluble fertilizer is 5: 95;
the water-soluble fertilizer is a macroelement water-soluble fertilizer and consists of urea, monopotassium phosphate and potassium sulfate according to the mass ratio of 50:20: 30.
Example 7
A preparation method of seaweed liquid comprises adding seaweed into sodium acetate buffer solution, performing enzymolysis with mixed enzyme, inactivating enzyme, and filtering to obtain filtrate, wherein the mixed enzyme is a composition of saccharifying enzyme, cellulase and bovine serum albumin at a mass ratio of 1:2: 15;
the pH of the sodium acetate buffer solution is 5.4;
the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 28:70: 2; the seaweed is kelp.
The enzymolysis condition of the mixed enzyme is 46 ℃; the enzymolysis time is 34 h.
The enzyme deactivation and enzyme deactivation conditions are that the temperature is 100 ℃ and the time is 2 min.
Wherein the activity of saccharifying enzyme is 50000U/g, the activity of cellulase is 100000U/g, and the enzyme is purchased from Novoxin (China) Biotechnology Co., Ltd.
The preparation method for preparing the seaweed fertilizer from the seaweed liquid comprises the following steps:
drying the seaweed solution at 60 deg.C until the water content is 1.8% to obtain seaweed powder;
and (3) conveying 2 parts of seaweed powder, 300 parts of urea, 200 parts of monoammonium phosphate, 300 parts of potassium sulfate and 200 parts of bentonite into a granulation roller through a metering belt, and granulating to obtain the seaweed fertilizer.
Example 8
A preparation method of seaweed liquid comprises the steps of adding seaweed into sodium acetate buffer solution, carrying out enzymolysis through mixed enzyme, carrying out enzyme deactivation to obtain saccharification enzymolysis liquid, adding compound protease into the saccharification enzymolysis liquid, carrying out enzymolysis, carrying out enzyme deactivation, and filtering to obtain filtrate, namely the seaweed liquid, wherein the mixed enzyme is a composition of the saccharifying enzyme, cellulase and bovine serum albumin according to the mass ratio of 1:2: 15;
the pH of the sodium acetate buffer solution is 5.4;
the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 28:70: 2; the seaweed is kelp.
The enzymolysis condition of the mixed enzyme is 46 ℃; the enzymolysis time is 34 h.
The mass ratio of the protease addition amount to the mixed enzyme addition amount is 1: 14; the enzymolysis condition of the protease is that the enzymolysis temperature is 32 ℃ and the enzymolysis time is 28 h.
The enzyme deactivation and enzyme deactivation conditions are that the temperature is 100 ℃ and the time is 2 min.
Wherein the activity of saccharifying enzyme is 50000U/g, the activity of cellulase is 100000U/g, the activity of compound protease is 93400U/g, and the enzyme is purchased from Novoxin (China) biotechnology limited.
The preparation method for preparing the seaweed fertilizer from the seaweed liquid comprises the following steps:
drying the seaweed solution at 60 deg.C until the water content is 1.8% to obtain seaweed powder;
and (3) conveying 2 parts of seaweed powder, 300 parts of urea, 200 parts of monoammonium phosphate, 300 parts of potassium sulfate and 200 parts of bentonite into a granulation roller through a metering belt, and granulating to obtain the seaweed fertilizer.
Example 9
A preparation method of seaweed liquid comprises the steps of adding seaweed into a sodium acetate buffer solution, carrying out enzymolysis through mixed enzyme, carrying out enzyme deactivation to obtain saccharified enzymolysis liquid, adding 6% of sodium hydroxide aqueous solution into the saccharified enzymolysis liquid to adjust the pH value to 7.0, adding compound protease, carrying out enzymolysis, carrying out enzyme deactivation, and filtering to obtain filtrate, namely the seaweed liquid, wherein the mixed enzyme is a composition of the saccharified enzyme, cellulase and bovine serum albumin according to the mass ratio of 1:2: 15;
the pH of the sodium acetate buffer solution is 5.2;
the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 28:70: 2; the seaweed is kelp.
The enzymolysis condition of the mixed enzyme is 46 ℃; the enzymolysis time is 34 h.
The mass ratio of the protease addition amount to the mixed enzyme addition amount is 1: 14; the enzymolysis conditions of the protease are that the enzymolysis temperature is 32 ℃ and the enzymolysis time is 28h
The enzyme deactivation and enzyme deactivation conditions are that the temperature is 100 ℃ and the time is 2 min.
Wherein the activity of saccharifying enzyme is 50000U/g, the activity of cellulase is 100000U/g, the activity of compound protease is 93400U/g, and the enzyme is purchased from Novoxin (China) biotechnology limited.
The preparation method for preparing the seaweed fertilizer from the seaweed liquid comprises the following steps:
drying the seaweed solution at 60 deg.C until the water content is 1.8% to obtain seaweed powder;
and (3) conveying 2 parts of seaweed powder, 300 parts of urea, 200 parts of monoammonium phosphate, 300 parts of potassium sulfate and 200 parts of bentonite into a granulation roller through a metering belt, and granulating to obtain the seaweed fertilizer.
The beneficial effects of the present invention are further illustrated below in conjunction with experimental data:
test material
1.1 test sites: the detection was made by Sanfeng (tobacco pipe) agriculture science and technology Co.
1.2 test detection: algal polysaccharide content (%).
1.3 test materials: comparative 1 (saccharified enzymatic hydrolysate prepared according to patent No. CN112661543A example 1), comparative 2 (saccharified enzymatic hydrolysate prepared in the same manner as in example 2 except that the sodium acetate buffer solution was changed to water), comparative 3 (saccharified enzymatic hydrolysate prepared in the same manner as in example 2 except that bovine serum albumin was not added), comparative 4 (saccharified enzymatic hydrolysate prepared in the same manner as in example 5 except that the sodium acetate buffer solution was changed to water and no bovine serum albumin was added, and enzymatic hydrolysis were sequentially performed, wherein the enzymatic hydrolysis condition was ph 6.0, the enzymatic hydrolysis temperature was 30 ℃, the enzymatic hydrolysis time was 10 hours, the enzymatic hydrolysis condition of the saccharification enzyme was ph 4.2, the enzymatic hydrolysis temperature was 60 ℃, the enzymatic hydrolysis time was 22 hours, enzyme deactivation was performed after each enzymatic hydrolysis, the enzymatic deactivation condition was 100 ℃, the enzymatic hydrolysis time was 3 minutes), comparative 5 (saccharified enzymatic hydrolysis was performed except that the sodium acetate buffer solution was changed to water, the same procedure as in example 5 was repeated except that the above-mentioned components were replaced by the above-mentioned components, comparative example 6 (the same saccharification enzymatic hydrolysate prepared in example 5 was repeated except that bovine serum albumin was not added), the saccharification enzymatic hydrolysate prepared in example 2, and the saccharification enzymatic hydrolysate prepared in example 5.
1.4 Experimental implementation: the seaweed polysaccharide is detected by a sulfuric acid-phenol method.
The present application is consistent in other implementations except for differences in the respective processes.
2 results and analysis
The content of algal polysaccharides is shown in Table 1
TABLE 1
| Comparative example 1 | Comparative example 2 | Comparison 3 | Comparative example 4 | Comparative example 5 | Comparative example 6 | Example 2 | Example 5 | |
| Algal polysaccharide (%) | 13.8 | 12.3 | 10.1 | 7.2 | 6.4 | 5.1 | 13.7 | 7.7 |
Note: in table 1, comparison 2, and comparison 3 … correspond to the saccharification enzymatic hydrolysate prepared in comparison 1, the saccharification enzymatic hydrolysate prepared in comparison 2, and the saccharification enzymatic hydrolysate … prepared in comparison 3, respectively; wherein the content of total sugar in Porphyra haitanensis is 47.2%; the kelp contains 31.1% of total sugar.
As can be seen from comparison 1 (extraction time 36 h) in Table 1, cellulase and glucoamylase are adopted for sequential extraction, and extraction for 36h is relatively complete; as can be seen from the comparison of the values of the comparison 2, the comparison 3 and the example 2, the extraction of the sodium acetate buffer solution and the bovine serum albumin is combined, and the extraction of the example 2 of the application is more complete at the same time; as can be seen from the comparison of the data of the comparison 1 and the data of the comparison 2, the time of the embodiment 2 of the method is saved by 6h compared with the comparison 1, the extraction rate is basically unchanged, and the extraction efficiency can be improved.
As can be seen from Table 1 and comparative example 4 (extraction time 32 h), the extraction of 32h by using cellulase and glucoamylase in sequence has the problem of incomplete extraction; as can be seen from the comparison of the values of the comparison 5, the comparison 6 and the example 5, the extraction of the example 5 of the application is more complete at the same time by the combined extraction of the sodium acetate buffer solution and the bovine serum albumin; as can be seen from the comparison of the data of comparative example 4 and example 5, the extraction of the application is more complete in the case of example 5 and comparative example 4 under the premise of the same extraction time.
To sum up, this application can show and improve extraction efficiency through adding sodium acetate buffer solution and bovine serum albumin.
Experiment two
1.1 test site: the detection was made by Sanfeng (tobacco pipe) agriculture science and technology Co.
1.2 test detection: total free amino acid content (%).
1.3 test materials: example 2, example 3, example 5 and example 6.
1.4 detection method: detection of free amino acid content the detection was carried out according to the determination of free amino acids in the GB/T30987-2014 plant.
The present application is consistent in other implementations except for differences in the respective processes.
2 results and analysis
The total content of free amino acids is shown in Table 2
TABLE 2
| Example 2 | Example 3 | Example 4 | Example 5 | |
| Total free amino acid content (%) | 4.8 | 6.5 | 0.4 | 1.5 |
From the data of examples 2 and 3 and examples 4 and 5, it can be seen that the total content of free amino acids can be increased by adjusting the pH of the saccharified enzymatic hydrolysate. This may be due to the recombination of carboxylic acid groups in bovine serum albumin with amino groups of free amino acids, which affects the extraction of free amino acids.
Experiment three
1, materials and methods:
1.1 test site: the apple variety is 3 in three acres of apple orchards in Su shop, Zhen Su shop and Zhen Su shop in Xixia city, the age of the apple orchard is 10 years, 60 apple trees are planted in each acre, and 160 apple trees with almost the same length are taken.
1.2 test detection: detecting the L, a, b, c and h values of the apples; and (4) averaging the fruit grades (d is more than or equal to 80 mm), and detecting for 10 months and 24 days in 2021 years, namely 12 days after the apples are bagged.
1.3 test materials: comparative example 7 (same as example 2 except that no seaweed liquid was added), comparative example 8 (same as example 2 except that CN112538511A was added to the seaweed liquid prepared in example 1), comparative example 9 (same as example 5 except that no seaweed liquid was added), comparative example 10 (same as example 5 except that CN112538511A was added to the seaweed liquid prepared in example 3), and water-soluble fertilizers prepared in examples 2, 3, 5 and 6.
1.4 Experimental methods: 160 fruit trees were grouped into 8 groups of 20 adjacent fruit trees, which were respectively corresponding to the water-soluble fertilizers prepared in comparative example 7 (same as example 2 except that no seaweed liquid was added), comparative example 8 (same as example 2 except that the seaweed liquid was added according to CN112538511A example 1), comparative example 9 (same as example 5 except that no seaweed liquid was added), comparative example 10 (same as example 5 except that the seaweed liquid was added according to CN112538511A example 3), example 2, example 3, example 5 and example 6; the water soluble fertilizer is used by micro-spraying, wherein, comparison 7 (except that no seaweed liquid is added, the other parts are the same as the embodiment 2), comparison 8 (except that the seaweed liquid is added with CN112538511A, the other parts are the same as the embodiment 2) and the embodiment 2 and the embodiment 3 are diluted by water with the dilution factor of 500; comparative example 9 (same as example 5 except that no seaweed liquid was added), comparative example 10 (same as example 5 except that the seaweed liquid was prepared in example 3 in accordance with CN 112538511A), and examples 5 and 6 were diluted with water at a dilution ratio of 1000; of these, comparative example 7 (same as example 2 except that no seaweed liquid was added), comparative example 8 (same as example 2 except that CN112538511A was added to the seaweed liquid prepared in example 1), and example 2 and example 3 each using 10kg of fertilizer per treatment; comparative example 9 (same as example 5 except that no seaweed liquid was added), comparative example 10 (same as example 5 except that the seaweed liquid was added with the seaweed liquid prepared in example 3 of CN 112538511A), example 5 and example 6, each treatment using 5kg of fertilizer. The experiment was performed from 3/31/2021 to 9/15/2021, with 9 treatments per treatment during the experiment.
1.5 detection method: in a CIELab model, L represents the color brightness, the lightness is in direct proportion to a numerical value, a represents red and green, b represents yellow and blue, c represents the chroma (color saturation or pure degree), and h represents the hue angle; performing hardness detection by using a hardness meter; randomly picking 100 apples per treatment for testing (requiring sampled apples to develop normally and without bird pecking), fruit grade = mass of apples of a certain diameter/total mass of apples participating in the calculation 100%, wherein the statistical diameter of coloration is (d) fruits of 75-80 mm; fruits with a fruit grade statistic diameter (d) of more than 80 mm.
The experiment was conducted in a consistent manner except for the different experimental treatments.
2 results and analysis
The color and fruit grade (d is more than or equal to 80 mm%) of the apples are shown in Table 3
TABLE 3
As can be seen from the data of examples 2 and 3 and examples 5 and 6 in tables 2 and 3, the adjustment of pH of the saccharified enzymatic hydrolysate before the enzymatic hydrolysis by protease affects not only the extraction of free amino acids but also the effect of preparing a fertilizer using the prepared algal solution, and examples 3 and 6, which have better effects by adjusting pH, have better effects.
As can be seen from the data of comparison 8 and example 3 and comparison 10 and example 6, the present application changes the extraction method of the seaweed liquid, but does not affect the use effect.
Claims (8)
1. A preparation method of seaweed liquid comprises adding seaweed and mixed enzyme into sodium acetate buffer solution, performing enzymolysis with the mixed enzyme, and filtering to obtain filtrate, which is seaweed liquid, wherein the mixed enzyme at least comprises diastase, cellulase and bovine serum albumin; the mass ratio of the seaweed to the sodium acetate buffer solution to the mixed enzyme is 19-39: 60-80: 0.07-0.5; the mass ratio of the saccharifying enzyme to the cellulase to the bovine serum albumin in the mixed enzyme is 1-2: 5-15; the pH value of the sodium acetate buffer solution is 4.4-6.0; the seaweed is one or more of porphyra haitanensis, kelp, agar and gulfweed;
the mixed enzyme enzymolysis condition is that the temperature is 37-50 ℃ and the time is 6-36 h.
2. The method for preparing seaweed liquid according to claim 1, further comprising inactivating enzyme after enzymolysis with mixed enzyme, and adding protease after inactivating enzyme for enzymolysis with protease; the mass ratio of the protease addition amount to the mixed enzyme addition amount is 1: 10-20; the enzymolysis condition of the protease is that the enzymolysis temperature is 26-35 ℃, and the enzymolysis time is 6-48 h;
the enzyme deactivation condition is that the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min.
3. The method for preparing seaweed liquid as claimed in claim 2, further comprising inactivating the enzyme after the enzymolysis with the protease; the enzyme deactivation and enzyme deactivation conditions are that the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min.
4. The method of preparing seaweed solution according to claim 2 or 3, wherein the protease enzymolysis further comprises adding one of sodium hydroxide, sodium carbonate, potassium carbonate or potassium hydroxide before the enzymolysis, and adjusting the pH to 7.0.
5. The method of claim 2 or 3, wherein the enzymatic hydrolysis with protease further comprises introducing nitrogen gas into the seaweed solution before the enzymatic hydrolysis until the oxygen content in the seaweed solution is less than 0.2 mg/L.
6. The method of claim 4, wherein the enzymatic hydrolysis with the protease further comprises introducing nitrogen gas into the seaweed solution before the enzymatic hydrolysis until the oxygen content in the seaweed solution is less than 0.2 mg/L.
7. The method for preparing the seaweed fertilizer from the seaweed liquid as claimed in any one of claims 1 to 6, which comprises the following steps:
drying the seaweed liquid to obtain seaweed powder; the drying temperature is not higher than 85 ℃;
adding the seaweed liquid or seaweed powder into the fertilizer or the fertilizer raw material to obtain the seaweed fertilizer;
the fertilizer is one of a water-soluble fertilizer, a compound fertilizer, an organic-inorganic fertilizer, a biological organic fertilizer, a simple substance fertilizer and an organic fertilizer;
the fertilizer raw materials are one or more of potassium chloride, potassium sulfate, urea, monoammonium phosphate, diammonium phosphate, ammonium sulfate, ammonium chloride, monopotassium phosphate, bentonite, zinc sulfate, ammonium calcium nitrate and potassium nitrate.
8. The method for preparing the seaweed fertilizer from the seaweed liquid as claimed in claim 7, wherein the mass ratio of the seaweed liquid to the fertilizer or the fertilizer raw materials is 3-30: 70-99;
the mass ratio of the seaweed powder to the fertilizer or the fertilizer raw materials is 1-10: 90-99.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210219470.1A CN114525316A (en) | 2022-03-08 | 2022-03-08 | Seaweed liquid and preparation method of seaweed fertilizer thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210219470.1A CN114525316A (en) | 2022-03-08 | 2022-03-08 | Seaweed liquid and preparation method of seaweed fertilizer thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114525316A true CN114525316A (en) | 2022-05-24 |
Family
ID=81627021
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210219470.1A Pending CN114525316A (en) | 2022-03-08 | 2022-03-08 | Seaweed liquid and preparation method of seaweed fertilizer thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114525316A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115677411A (en) * | 2022-11-16 | 2023-02-03 | 山东爱果者生物技术有限公司 | Water-soluble fertilizer and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103224967A (en) * | 2013-04-15 | 2013-07-31 | 沈阳师范大学 | Preparation method of low galactose content guar gum |
| WO2019217489A1 (en) * | 2018-05-08 | 2019-11-14 | Nutriomix, Inc. | Seaweed meal and method of making the same |
| CN112479782A (en) * | 2021-01-15 | 2021-03-12 | 晟丰(烟台)农业科技有限公司 | Seaweed fertilizer and preparation method thereof |
| CN112538511A (en) * | 2021-01-26 | 2021-03-23 | 晟丰(烟台)农业科技有限公司 | Extraction method of seaweed liquid |
| CN112661543A (en) * | 2021-01-26 | 2021-04-16 | 晟丰(烟台)农业科技有限公司 | Seaweed fertilizer and preparation method thereof |
| CN112813117A (en) * | 2021-03-04 | 2021-05-18 | 天津科技大学 | Method for improving enzymolysis efficiency by cooperatively treating poplar through steam explosion and bovine serum albumin |
-
2022
- 2022-03-08 CN CN202210219470.1A patent/CN114525316A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103224967A (en) * | 2013-04-15 | 2013-07-31 | 沈阳师范大学 | Preparation method of low galactose content guar gum |
| WO2019217489A1 (en) * | 2018-05-08 | 2019-11-14 | Nutriomix, Inc. | Seaweed meal and method of making the same |
| CN112479782A (en) * | 2021-01-15 | 2021-03-12 | 晟丰(烟台)农业科技有限公司 | Seaweed fertilizer and preparation method thereof |
| CN112538511A (en) * | 2021-01-26 | 2021-03-23 | 晟丰(烟台)农业科技有限公司 | Extraction method of seaweed liquid |
| CN112661543A (en) * | 2021-01-26 | 2021-04-16 | 晟丰(烟台)农业科技有限公司 | Seaweed fertilizer and preparation method thereof |
| CN112813117A (en) * | 2021-03-04 | 2021-05-18 | 天津科技大学 | Method for improving enzymolysis efficiency by cooperatively treating poplar through steam explosion and bovine serum albumin |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115677411A (en) * | 2022-11-16 | 2023-02-03 | 山东爱果者生物技术有限公司 | Water-soluble fertilizer and preparation method thereof |
| CN115677411B (en) * | 2022-11-16 | 2023-10-27 | 湖南沃肥生物科技有限公司 | Water-soluble fertilizer and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112661543B (en) | Seaweed fertilizer and preparation method thereof | |
| CN107032886B (en) | Selenium-rich natural multifunctional foliar fertilizer and preparation method thereof | |
| CN112479782A (en) | Seaweed fertilizer and preparation method thereof | |
| CN112538511B (en) | Extraction method of seaweed liquid | |
| CN105061109B (en) | A kind of sub-micron organic carbon fertilizer and its production method | |
| CN113321563A (en) | Seaweed organic water-soluble fertilizer | |
| CN111574255A (en) | Method for producing organic water-soluble fertilizer by using straws | |
| CN114525316A (en) | Seaweed liquid and preparation method of seaweed fertilizer thereof | |
| CN114368987A (en) | Soil activation type microbial fertilizer and preparation method thereof | |
| CN115094107A (en) | Method for preparing soybean polypeptide composition and polypeptide amino acid fertilizer and product | |
| CN105085025A (en) | Water-soluble fertilizer capable of improving color and luster of apples and promoting coloring and application method | |
| CN102838424A (en) | Biological humic acid urea | |
| CN104193535A (en) | Environment-friendly organic and inorganic fertilizer prepared by taking molasses alcohol waste mash as raw material | |
| CN113880639A (en) | Humic acid fertilizer and preparation method thereof | |
| AU2020104406A4 (en) | Citric acid organic foliar fertilizer, and preparation method and use thereof | |
| CN114276197A (en) | Method for producing synergistic fertilizer by using food waste | |
| CN112680361A (en) | Trichoderma galnarum and application thereof | |
| CN114989984B (en) | Method for preparing seaweed extract by enzymolysis | |
| CN114315483A (en) | Humic acid water-soluble fertilizer containing seaweed extract and preparation method thereof | |
| CN105753516A (en) | Method of manufacture and method of use of biochar capable of promoting growth of seedlings in gleyed rice field | |
| CN114874043A (en) | Compound bio-organic fertilizer for improving saline-alkali soil and preparation method thereof | |
| CN113862324A (en) | Preparation method of mixed liquid of polypeptide and amino acid, amino acid foliar fertilizer and polypeptide water-soluble fertilizer | |
| CN104193455A (en) | Organic and inorganic fertilizer prepared by taking molasses alcohol waste mash as raw material | |
| CN105732226A (en) | Suspension type seedling-lifting liquid compound fertilizer and preparation method thereof | |
| CN113564053A (en) | Trichoderma hedgehog and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |