CN112618733A

CN112618733A - Scutellaria baicalensis test method based on hypertensive mice, and liquid medicine storage device and method

Info

Publication number: CN112618733A
Application number: CN202011527074.2A
Authority: CN
Inventors: 李亚芹; 解诗语; 王乾一; 康世鑫; 崔红根
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-12-22
Filing date: 2020-12-22
Publication date: 2021-04-09
Anticipated expiration: 2040-12-22
Also published as: CN112618733B

Abstract

The invention relates to a scutellaria baicalensis test method based on hypertensive mice, which comprises the steps of preparing experimental animals, namely spontaneous hypertensive rats, 10 Wister rats and a cleaning level; experimental medications, scutellaria baicalensis and benazepril; a kit; the device comprises an experimental instrument, a water bath constant temperature oscillator, a vortex mixer, a centrifuge, a thermostat, an enzyme-labeling instrument, an electronic analysis balance, an animal dissection instrument and an experimental bench; also relates to a liquid medicine storage device and a method, comprising a liquid medicine carrier (1) for packaging liquid medicine; the device comprises a station conveying device (2); the station conveying device (2) comprises a conveying line (15) distributed with conveying clamping tools (16); the transfer mould (16) is a hollow structure, and the upper port of the transfer mould is provided with a mould process notch (17); the invention has reasonable design, compact structure and convenient use.

Description

Scutellaria baicalensis test method based on hypertensive mice, and liquid medicine storage device and method

Technical Field

The invention relates to a scutellaria baicalensis test method based on hypertensive mice, a liquid medicine storage device and a liquid medicine storage method.

Background

Scutellaria baicalensis is Labiatae (labiatae) and was first recorded in Shen nong's herbal Jing, and has the effects of clearing heat, purging fire, detoxifying, stopping bleeding, preventing miscarriage and the like. The chemical components of the scutellaria baicalensis mainly contain flavonoid components, and in addition, the scutellaria baicalensis also contains sitosterol, daucosterol, trace elements and the like, and the main pharmacological effects of the scutellaria baicalensis have the aspects of antibiosis, antioxidation, anti-inflammation, anti-allergic reaction, tumor resistance and the like. At present, the research is mostly limited to the anti-tumor effect, the anti-virus effect, the liver protection effect and the in vitro anti-oxidation effect of the scutellaria baicalensis. The WangQ research finds that the effective components of the scutellaria have the inhibition effect on myocardial fibrosis and collagen hyperplasia, so that the effective components of the scutellaria have certain in-vitro antioxidant, myocardial fibrosis resistant and collagen hyperplasia resistant effects. The invention aims to verify the influence of scutellaria on the collagen content in myocardial tissues.

In addition, along with the problem of environmental pollution, the glass medicament bottle cannot be degraded by nature, is high in cost and is fragile and gradually eliminated by plastics.

Disclosure of Invention

The invention aims to solve the technical problem of providing a scutellaria baicalensis testing method based on hypertensive mice, a liquid medicine storage device and a liquid medicine storage method.

In order to solve the problems, the technical scheme adopted by the invention is as follows:

a Scutellariae radix test method based on hypertensive mice, S1, preparing experimental animals of 10 Wister rats with spontaneous hypertension, cleaning level; experimental medications, scutellaria baicalensis and benazepril; a kit; the device comprises an experimental instrument, a water bath constant temperature oscillator, a vortex mixer, a centrifuge, a thermostat, an enzyme-labeling instrument, an electronic analysis balance, an animal dissection instrument and an experimental bench;

s2, experimental procedure:

s2.1, randomly dividing the spontaneous hypertension rats into the following groups: benazepril group, scutellaria group, model group and blank control group, the scutellaria group is divided into high, middle and low three groups;

s2.2, respectively administering the benazepril, the effective components of the scutellaria baicalensis, the model group and the spontaneous hypertension rats of the control group with the same amount of physiological saline for intragastric administration, and simultaneously feeding standard feed and free drinking water;

s3, measurement of blood pressure and heart rate:

firstly, preheating the tail of a rat in water of 40 ℃ and then measuring the blood pressure; then, directly and synchronously recording the pulse and pressure signals through a processing system; secondly, continuously carrying out N times of measurement, taking the average value as the systolic pressure and the heart rate of the sample, wherein the unit is respectively as follows: mmHg and bpm; thirdly, the results are obtained, and the blood pressure of the rats in the model group and the treatment group is obviously higher than that of the rats in the blank group.

As a further improvement of the above technical solution:

measurement of collagen content in myocardial tissue:

firstly, anesthetizing a rat by using 3% sodium pentobarbital, fixing the rat on an anatomical table, then dissecting the chest, taking out the heart, and precooling the heart in normal saline; then, separating the left ventricle, and washing the left ventricle with precooled physiological saline; secondly, sucking water by using clean filter paper, homogenizing the myocardial tissues of the rats of each group, and then determining the contents of the carboxyl terminal peptide of the type I procollagen and the type III procollagen in the myocardial tissues, wherein the determination method adopts an enzyme-linked immunosorbent assay;

s5, histological examination: fixing a little of myocardial tissue by 10% formaldehyde, washing with 0.01MPBS phosphate buffered normal saline for 3 times after 48 hours, performing gradient dehydration by 70-100% alcohol, performing transparency in xylene for 2 times, embedding paraffin, fixing the repaired wax block on a paraffin slicer for slicing, wherein the thickness is 7um, and observing the change of the myocardial tissue structure by using a microscope after HE staining;

s6, statistical methods;

firstly, the data obtained by the experiment are subjected to statistical analysis, the data are analyzed by adopting an SPSS12.0 software package, the data are all expressed by x +/-s, and variance analysis is adopted for comparison among a plurality of groups of samples.

Results were compared to the change in blood pressure and heart rate before and after the rat experiment.

A liquid medicine storage device is used for packaging liquid medicine in a liquid medicine carrier; the device comprises a station conveying device; the station conveying device comprises a conveying line distributed with conveying clamping tools; the transfer mould is a hollow structure, and the upper port of the transfer mould is provided with a mould process notch;

distributed on the transmission line are:

a falling station with a falling device for placing the liquid medicine carrier on the conveying clamping fixture;

the adjusting station is provided with a pressing manipulator; pressing the manipulator downwards to place the liquid medicine carrier with edges and an opening at the upper end in a conveying mould;

the cleaning station is provided with an ozone blowing pipe and is used for introducing ozone gas into the liquid medicine carrier for disinfection;

a filling station having a filling machine for filling the liquid medicine into the liquid medicine carrier;

the film sealing station is provided with a film sealing device and is used for sealing the upper port of the liquid medicine carrier;

a labeling station having a labeling device for labeling the sealing film for discrimination;

and/or the output station is used for outputting the liquid medicine carrier fully loaded with the liquid medicine.

A liquid medicine storage device comprises a falling device positioned at a falling station; the device comprises a falling guide part vertically arranged right above a falling station and a falling stop lever arranged below the falling guide part; a falling process gap is formed between the lower part of the falling guide part and the upper part of the falling blocking rod; the liquid medicine carrier is stacked in the falling guide part;

a falling crankshaft part is arranged on one horizontal side of the falling process gap; the falling crankshaft part is hinged with a falling U-shaped pushing frame through a falling connecting rod part; a guide rod of the falling U-shaped pushing frame linearly reciprocates in the falling telescopic guide part;

a fork head of the falling U-shaped pushing frame is provided with a falling wedge, and the falling wedge is used for entering and exiting from a neutral position between the edges of the liquid medicine carriers at the stacking bottom of the falling technical gap;

a falling speed distribution part is connected to the falling crankshaft part through a falling bevel gear set in a transmission manner, a falling stirring blade is connected to the falling speed distribution part in a transmission manner, and a falling stirring process gap matched with the liquid medicine carrier is formed in the falling stirring blade;

the falling poking blade is inserted into a neutral position between the edges of the liquid medicine carriers at the stacking bottom.

As a further improvement of the above technical solution:

dropping and pulling the edge of the bottommost liquid medicine carrier of the blade support liquid medicine carrier;

at the falling process gap, the falling crankshaft part drives the falling U-shaped pushing frame to linearly reciprocate in the falling telescopic guide part through the falling connecting rod part, so that the falling wedge enters and exits at a neutral position between the bottommost layer and the edge of the liquid medicine carrier on the second lower layer, the liquid medicine carrier on the second lower layer is separated from the liquid medicine carrier on the bottommost layer by utilizing the inclination, and meanwhile, the falling stirring blade swings downwards to enable the liquid medicine carrier on the bottommost layer to fall;

and the falling stirring blade circulates to continue to come under the liquid medicine carrier at the bottommost layer, and meanwhile, the falling speed is larger than that of the inclined wedge which retreats away from the neutral position, so that the stacked liquid medicine carriers fall onto the falling stirring blade.

A liquid medicine storage device comprises a film sealing device, a film sealing device and a liquid medicine storage device, wherein the film sealing device comprises a film sealing feeding coil and a film sealing discharging reel which are positioned at two transverse sides above a film sealing station; the film sealing upper material roll conveys a film sealing strip to the film sealing damping counterweight roller group, and the film sealing strip passes through the upper part of the liquid medicine carrier positioned at the film sealing station; a film sealing damping counterweight roller set is matched and sleeved on the film sealing strip;

a first film sealing swing arm and a second film sealing swing arm are arranged on the side of the film sealing station, and the roots of the first film sealing swing arm and the second film sealing swing arm are respectively hinged to a frame of the film sealing device;

a film sealing linkage swing arm is hinged between the cantilever end of the film sealing first swing arm and the cantilever end of the film sealing second swing arm, and a film sealing die cutter and a film sealing thermoplastic welding head are arranged at the end part of the film sealing linkage swing arm;

when the film sealing die-cutting knife and the film sealing thermoplastic welding joint are downward, the first film sealing swing arm and the second film sealing swing arm are in an X shape; when the film sealing die-cutting knife and the film sealing thermoplastic welding joint face upwards, the film sealing first swing arm, the film sealing second swing arm, the film sealing linkage swing arm and the rack form a quadrilateral structure;

the film sealing upper material roll conveys a film sealing strip for sealing the film to a film sealing discharge scroll and winds the die-cut film sealing strip after passing through a film sealing damping balance roller set;

the first film sealing swing arm drives the second film sealing swing arm to be in linkage or the first film sealing swing arm drives the second film sealing swing arm to be in linkage, the film sealing die cutting knife performs die cutting on the film sealing strip, and the film sealing thermoplastic welding head performs die cutting on the film sealing strip and the edge of the liquid medicine carrier.

A liquid medicine storage device comprises a label device, wherein the label device comprises a first label swing arm which is positioned above a label station and the root of which is hinged on a rack of the label device; a label linkage arm is hinged to the upper end of the first label swing arm, and a label printing head for printing a seal film of the liquid medicine carrier is arranged at the lower end of the label linkage arm;

the middle part of the label linkage arm is hinged with the head end part of a second label swing arm, and the middle part of the second label swing arm is hinged on the rack;

when the label printing head prints downwards, the first label swing arm and the second label swing arm are in an X-shaped structure, and when the label printing head is upward to be inked, the first label swing arm is positioned on the front side of the second label swing arm and forms a quadrilateral structure with the label linkage arm and the rack;

the tail end of the second swing arm of the label is hinged with a label driven connecting rod, the label driven connecting rod is connected with a label lifting rod, one side of the label lifting rod is provided with a label ink box, the label ink applying brush is arranged on the label lifting rod, and when a label printing head prints characters downwards, the label lifting rod is used for dipping ink at the label ink box and when the label printing head is upwards to be used for being inked by the ink applying head which overturns upwards.

A liquid medicine storage method is used for respectively filling required Scutellariae radix liquid medicine or benazepril in a liquid medicine carrier; by means of a station conveyor; the method comprises the following steps of;

s1, placing the liquid medicine carrier on the conveying mould through the falling device at the falling station;

s2, at the adjusting station, firstly, receiving the liquid medicine carrier sent by the falling station; then, the liquid medicine carrier with edges and an opening at the upper end is horizontally placed in a conveying mould by a downward pressing manipulator;

s3, at the cleaning station, firstly, receiving the liquid medicine carrier sent by the adjusting station; then, ozone gas is introduced into the liquid medicine carrier through an ozone blowing pipe for disinfection;

s4, at the filling station, firstly, receiving the liquid medicine carrier sent by the cleaning station; then, filling the liquid medicine into the liquid medicine carrier through a filling machine;

s5, at the film sealing station, firstly, receiving the liquid medicine carrier sent by the filling station; then, sealing the upper port of the liquid medicine carrier by a film sealing device;

s6, at the label station, firstly, receiving the liquid medicine carrier sent by the film sealing station; then, labeling is carried out on the sealing film through a labeling device;

s7, at the output station, firstly, the liquid medicine carrier sent by the label station is received; then, the chemical liquid carrier full of the chemical liquid is output.

A liquid medicine storage method comprises step S1, the specific steps are as follows;

s1.1, pre-storing stacked liquid medicine carriers in a falling guide part, and falling to stir blades to lift two sides of the lowest layer;

s1.2, at the position of a falling process gap, a falling crankshaft part drives a falling U-shaped pushing frame to linearly reciprocate in a falling telescopic guide part through a falling connecting rod part; then, the falling wedge enters a neutral position between the bottom layer and the edge of the liquid medicine carrier of the second lower layer, the upper top of the liquid medicine carrier of the second lower layer is separated from the liquid medicine carrier of the bottom layer by utilizing the inclination, and meanwhile, the falling wedge stirs the blade to swing downwards, so that the liquid medicine carrier of the bottom layer falls into the conveying mould;

s1.3, enabling the falling stirring blades to circulate, continuing to come under the medicine liquid carrier at the bottommost layer, and enabling the falling stirring blades to retreat away from a neutral position when the falling stirring blades fall, so that the stacked medicine liquid carriers fall on the falling stirring blades and are supported;

s1.4, the station conveying device drives the conveying clamping fixture loaded with the liquid medicine carrier to leave the falling station.

A liquid medicine storage method comprises step S5, the specific steps are as follows;

s5.1, rotating a film sealing discharge reel to drive a film sealing strip output by a film sealing material loading roll to pass through the upper part of a film sealing station and then be wound on the film sealing discharge reel;

s5.2, the filled liquid medicine carrier comes to a film sealing station;

s5.3, the first film sealing swing arm and the second film sealing swing arm are in driving linkage, so that the film sealing die cutting knife and the film sealing thermoplastic welding head do downward turntable movement;

s5.4, die cutting is carried out on the film sealing strip on the liquid medicine carrier by the film sealing die-cutting cutter, and the film sealing thermoplastic welding head welds the film sealing strip on the edge of the liquid medicine carrier for sealing;

s5.5, the film sealing die-cutting knife and the film sealing thermoplastic welding head move upwards to turn over and leave the film sealing station;

s5.6, the filled liquid medicine carrier leaves a film sealing station; the film sealing discharge reel rotates to continuously drive a film sealing strip output by a film sealing feeding roll to be wound on the film sealing discharge reel after passing through the upper part of the film sealing station.

A liquid medicine storage method comprises step S6, the specific steps are as follows;

s6.1, the station conveying device drives the conveying mould loaded with the liquid medicine carrier to move to a label station;

s6.2, at the position where the label printing head faces upwards and is to be inked, the label inking brush is used for inking the label printing head;

s6.3, the first label swing arm and the second label swing arm swing, and the label printing head prints characters downwards on the sealing film of the liquid medicine carrier; the label inking brush dips ink at the label ink box;

s6.4, the first label swing arm and the second label swing arm swing to enable the label printing head to move upwards;

s6.5, the station conveying device drives the conveying clamping fixture loaded with the liquid medicine carrier to leave the label station.

Drawings

FIGS. 1(a) to 1(f) are schematic views of each set of myocardial tissue sections according to the present invention.

Fig. 2 is a schematic structural view of the station conveying device.

FIG. 3 is a schematic view of the drop device of the present invention.

Fig. 4 is a schematic view of the structure of the label device of the present invention.

Fig. 5 is a schematic view of the structure of the label device of the present invention.

Wherein: 1. a liquid medicine carrier; 2. a station conveying device; 3. a drop station; 4. adjusting the station; 5. cleaning the station; 6. a filling station; 7. a film sealing station; 8. a label station; 9. an output station; 10. a dropping device; 11. an ozone blowing pipe; 12. filling machine; 13. a film sealing device; 14. a label device; 15. a conveyor line; 16. conveying the clamping fixture; 17. a mold process notch; 18. a drop guide section; 19. a drop process gap; 20. a drop stop lever; 21. a lower crankshaft part; 22. a drop link section; 23. a drop telescopic guide part; 24. a falling U-shaped pushing frame; 25. falling is greater than the wedge; 26. a drop bevel gear set; 27. a falling speed matching part; 28. dropping and stirring the blade; 29. a drop and pick process gap; 30. sealing the film and feeding the material roll; 31. a film sealing damping balance weight roller set; 32. a film sealing and discharging reel; 33. sealing the membrane strip; 34. a first swing arm for sealing the film; 35. a second swing arm for sealing the film; 36. sealing the film and linking the swing arm; 37. sealing the film die-cutting rule; 38. sealing a thermoplastic welding head of the film; 39. a first swing arm of the label; 40. a label linkage arm; 41. a label print head; 42. a second swing arm of the label; 43. a tag driven link; 44. a label lifter; 45. a label inking brush; 46. a label cartridge.

Detailed Description

Example 1: the scutellaria baicalensis test method based on the hypertensive mice comprises the following steps of:

14 weeks old spontaneous hypertensive rats 50, common Wister rats 10, body weight 257 ± 10 grams, cleaning grade, birth place: a certain experimental animal center. Certification number 805108.

Experimental medicine and main reagent of radix Scutellariae. Benazepril. A kit.

An experimental instrument:

a water bath constant temperature oscillator, a vortex mixer IKA-MSZ type, a low-speed desk type centrifuge, a super thermostat, a Stat-Fax-2100 microplate reader, a high-speed refrigerated centrifuge, an electronic analytical balance, an animal dissection instrument and an experimental bench.

The experimental process comprises the following steps: spontaneous hypertensive rats were randomly divided into the following groups: benazepril group, scutellaria group (high, medium and low three doses) and model group, each group contains 10. Blank control group 10. The drug components are respectively given 5mg/kg/d benazepril, 17.5, 35 and 70mg/kg/d effective components of scutellaria baicalensis, and the model group and the control group are given equal amount of normal saline for intragastric administration for 8 weeks, and are fed with standard feed and free drinking water.

Blood pressure and heart rate measurements

Blood pressure (in awake and non-irritated states) was measured after preheating the rat tail for 15 minutes at 40 degrees in water. The pulse and pressure signals are directly and synchronously recorded by a processing system. The measurements were performed 3 times in succession, and the mean values were taken as the systolic blood pressure and heart rate of the sample, in units: mmHg and bpm. The blood pressure of the model group and the treatment group rats is obviously higher than that of the blank group.

Determination of collagen content in myocardial tissue

Anesthetizing a rat by using 3% sodium pentobarbital, fixing the rat on an anatomical table, then cutting open the chest, taking out the heart, precooling the heart in normal saline, separating the left ventricle, washing the left ventricle by using the precooled normal saline, then sucking water by using clean filter paper, homogenizing the myocardial tissues of the rat of each group, and then determining the contents of the type I procollagen carboxyl terminal peptide and the type III procollagen in the myocardial tissues, wherein the determination method adopts an enzyme-linked immunosorbent assay.

Histological examination: fixing a little of myocardial tissue by 10% formaldehyde, washing for 3 times by 0.01MPBS (phosphate buffered saline) after 48 hours, dehydrating by 70-100% alcohol with conventional concentration gradient, clearing in dimethylbenzene for 2 times, embedding in paraffin, fixing the repaired wax block on a paraffin slicer, slicing to a thickness of 7um, staining by conventional HE, and observing the change of the myocardial tissue structure by a microscope.

Statistical method

And (4) carrying out statistical analysis on the data obtained by the experiment, and carrying out data analysis by adopting an SPSS12.0 software package. Data are expressed in x + -s. The multiple sets of samples were compared using analysis of variance.

Results

Variation of blood pressure and heart rate before and after rat experiment

The experimental result shows that the 14-week-old spontaneous hypertensive rat has the systolic pressure obviously higher than that of a blank group of rats (P <0.01) at the same week age, the systolic pressure of a model group of rats at 22 weeks age is obviously higher than that of a 14-week-old rat (P <0.05), the systolic pressure of a scutellaria baicalensis group of rats at 22 weeks age is higher than that of the rats before treatment, but the systolic pressure of a high-dose group and the systolic pressure of a medium-dose group are obviously lower than that of the model group of rats at the same week age (P <0.01), no obvious difference exists between the two groups, and the systolic pressure of a low-. The contraction pressure of the benazepril group is reduced compared with that before treatment, and is obviously lower than that of the rats in the same week old model group (P < 0.01). The scutellaria baicalensis group and the model group have significant meaning (P is less than 0.01) in the reduction of systolic pressure; comparison of rat heart rates between groups and between groups showed no significant change in heart rate between groups, between groups before and after treatment (P > 0.05).

Effect of Scutellaria baicalensis Georgi effective component on content of type I procollagen carboxyl terminal peptide and type III procollagen amino terminal peptide (measured by ELISA) in rat myocardium

The type I procollagen carboxyl terminal peptide and the type III procollagen amino terminal peptide in serum can reflect myocardial collagen content. Compared with the normal group, the content of myocardial collagen in the model group is obviously increased (P <0.05), which indicates that the model group has obvious myocardial fibrosis; compared with a blank group, the data of the medicine group has no significant difference (P is more than 0.05); compared with the model group, the medium and high dose scutellaria baicalensis group and the benazepril group have obviously reduced myocardial collagen content (P is less than 0.05), and compared with the model group, the low dose group has no significant difference. Compared with the benazepril group, the scutellaria baicalensis group with medium and high dose has no significant difference (P > 0.05).

Normal rats have narrow myocardial spaces and are occupied by a small amount of collagen; the myocardial gap of the rat in the hypertension model group is obviously widened, the collagen content in the myocardial gap is obviously increased, and the rat is tightly wrapped with myocardial cells in palisade arrangement in different degrees; the collagen in the myocardial gap after the treatment of the scutellaria baicalensis with medium and high dose and the benazepril is obviously reduced compared with that of a model group, and the myocardial structure of a low-dose scutellaria baicalensis group is not obviously improved.

Each group of myocardial tissue sections: as shown in FIG. 1, normal rats have narrow myocardial spaces and are occupied by a small amount of collagen; the myocardial gap of the rat in the hypertension model group is obviously widened, the collagen content in the myocardial gap is obviously increased, and the rat is tightly wrapped with myocardial cells in palisade arrangement in different degrees; the collagen in the myocardial gap after the treatment of the scutellaria baicalensis with medium and high dose and the benazepril is obviously reduced compared with that of a model group, and the myocardial structure of a low-dose scutellaria baicalensis group is not obviously improved.

Embodiment 2, as shown in fig. 2 to 5, a liquid medicine storing device of the present embodiment is used for packaging a liquid medicine in a liquid medicine carrier 1; the device comprises a station conveying device 2; the station conveying device 2 comprises a conveying line 15 distributed with conveying clamping tools 16; the transfer mould 16 is a hollow structure, and the upper port of the transfer mould is provided with a mould process notch 17;

distributed over the conveyor line 15 are:

a dropping station 3 having a dropping device 10 to place the liquid medicine carrier 1 on a transfer mold 16;

an adjusting station 4 with a pressing manipulator; pressing down the manipulator to place the liquid medicine carrier 1 with edge and upper end opening in the conveying clamping fixture 16;

the cleaning station 5 is provided with an ozone blowing pipe 11 for introducing ozone gas into the liquid medicine carrier 1 for disinfection;

a filling station 6 having a filling machine 12 for filling the liquid medicine into the liquid medicine carrier 1;

the film sealing station 7 is provided with a film sealing device 13 and is used for sealing the upper port of the liquid medicine carrier 1;

a labelling station 8 with labelling means 14 for labelling the envelope for distinction;

and/or an output station 9 for outputting the liquid medicine carrier 1 fully loaded with the liquid medicine.

The liquid medicine storage device of the embodiment comprises a falling device 10 positioned at a falling station 3; it comprises a falling guide part 18 vertically arranged right above the falling station 3 and a falling stop lever 20 arranged below the falling guide part 18; a drop process gap 19 is formed between the lower part of the drop guide part 18 and the upper part of the drop stop rod 20; the liquid medicine carrier 1 is stacked in the drop guide 18;

a falling crankshaft part 21 is arranged on one horizontal side of the falling process gap 19; the falling crankshaft part 21 is hinged with a falling U-shaped pushing frame 24 through a falling connecting rod part 22; the guide rod of the falling U-shaped pushing frame 24 linearly reciprocates in the falling telescopic guide part 23;

a falling & gt wedge 25 is arranged on the fork head of the falling U-shaped pushing frame 24, and the falling & gt wedge 25 is used for entering and exiting from a neutral position between the edges of the liquid medicine carriers 1 at the stacking bottom at the falling process gap 19;

a falling speed distribution part 27 is in transmission connection with the falling crankshaft part 21 through a falling bevel gear set 26, a falling toggle blade 28 is in transmission connection with the falling speed distribution part 27, and a falling toggle technological gap 29 which is matched with the liquid medicine carrier 1 is arranged on the falling toggle blade 28;

the falling toggle blade 28 is inserted into the empty space between the edges of the liquid medicine carriers 1 at the bottom of the stack.

The falling poking blade 28 holds the edge of the bottommost liquid medicine carrier 1 of the liquid medicine carrier 1;

at the falling process gap 19, the falling crankshaft part 21 drives the falling U-shaped pushing frame 24 to linearly reciprocate in the falling telescopic guide part 23 through the falling connecting rod part 22, so that the falling wedge 25 enters and exits the neutral position between the bottommost layer and the edge of the liquid medicine carrier 1 on the lower second layer, the liquid medicine carrier 1 on the lower second layer is separated from the liquid medicine carrier 1 on the bottommost layer by utilizing the inclination, and meanwhile, the falling toggle blade 28 swings downwards, so that the liquid medicine carrier 1 on the bottommost layer falls;

the falling toggle blade 28 circulates to continue to the bottom of the medicine liquid carrier 1, and simultaneously, the falling > wedge 25 retreats away from the neutral position, so that the stacked medicine liquid carriers 1 fall onto the falling toggle blade 28.

The liquid medicine storage device of the embodiment comprises a film sealing device 13, which comprises a film sealing feeding roll 30 and a film sealing discharging reel 32 which are positioned at the two transverse sides above a film sealing station 7; the film sealing upper material roll 30 transmits a film sealing strip 33 to the film sealing damping balance weight roll group 31, and the film sealing strip 33 passes through the upper part of the liquid medicine carrier 1 positioned at the film sealing station 7; the film sealing strip 33 is matched with a film sealing damping counterweight roller set 31;

a first film sealing swing arm 34 and a second film sealing swing arm 35 with the roots hinged to the frame of the film sealing device 13 are arranged on the side of the film sealing station 7;

a film sealing linkage swing arm 36 is hinged between the cantilever ends of the first film sealing swing arm 34 and the second film sealing swing arm 35, and a film sealing die cutting knife 37 and a film sealing thermoplastic welding head 38 are arranged at the end part of the film sealing linkage swing arm 36;

when the film sealing die-cutting knife 37 and the film sealing thermoplastic welding head 38 face downwards, the film sealing first swing arm 34 and the film sealing second swing arm 35 are in an X shape; when the film sealing die-cutting knife 37 and the film sealing thermoplastic welding head 38 face upwards, the film sealing first swing arm 34, the film sealing second swing arm 35, the film sealing linkage swing arm 36 and the rack form a quadrilateral structure;

the film sealing upper material roll 30 conveys a film sealing strip 33 for film sealing to a film sealing discharge reel 32 after passing through a film sealing damping balance roller set 31 and winds the die-cut film sealing strip 33;

the first film sealing swing arm 34 drives the second film sealing swing arm 35 to move in a linked mode or the first film sealing swing arm 34 moves in a linked mode and the second film sealing swing arm 35 drives the film sealing die-cutting knife 37 to die-cut the film sealing strip 33, and the film sealing thermoplastic welding head 38 thermoplastically molds the die-cut film to the edge of the liquid medicine carrier 1.

The liquid medicine storage device of the embodiment comprises a label device 14, wherein the label device 14 comprises a first label swing arm 39 which is positioned above a label station 8 and is hinged on a rack of the label device 14 at the root part; a label linkage arm 40 is hinged at the upper end of the first label swing arm 39, and a label printing head 41 for printing a seal film of the liquid medicine carrier 1 is arranged at the lower end of the label linkage arm 40;

the middle part of the label linkage arm 40 is hinged with the head end part of a second label swing arm 42, and the middle part of the second label swing arm 42 is hinged on the rack;

when the label printing head 41 prints downwards, the first label swing arm 39 and the second label swing arm 42 are in an X-shaped structure, and when the label printing head 41 is upwards to be inked, the first label swing arm 39 is positioned on the front side of the second label swing arm 42 and forms a quadrilateral structure with the label linkage arm 40 and the frame;

a label follower link 43 is hinged to a rear end of the second label swing arm 42, a label lifting rod 44 is connected to the label follower link 43, a label cartridge 46 is disposed on one side of the label lifting rod 44, and a label inking brush 45 is disposed on the label lifting rod 44 for dipping ink in the label cartridge 46 when the label printing head 41 prints downward and for inking the label printing head 41 that is turned upward when the label printing head 41 prints upward to be inked.

The liquid medicine storage method of the embodiment is used for respectively filling the required scutellaria baicalensis liquid medicine or benazepril into the liquid medicine carrier 1; by means of the station conveyor 2; the method comprises the following steps of;

s1, placing the chemical liquid carrier 1 on the transfer mold 16 by the dropping device 10 at the dropping station 3;

s2, at the adjusting station 4, firstly, the liquid medicine carrier 1 sent by the falling station 3 is received; then, the liquid medicine carrier 1 with edges and an opening at the upper end is flatly placed in the conveying mould 16 through a downward pressing manipulator;

s3, at the cleaning station 5, firstly, the liquid medicine carrier 1 sent by the adjusting station 4 is received; then, ozone gas is introduced into the liquid medicine carrier 1 through the ozone blowing pipe 11 for disinfection;

s4, at the filling station 6, firstly, the liquid medicine carrier 1 sent by the cleaning station 5 is received; then, the liquid medicine is filled into the liquid medicine carrier 1 by the filling machine 12;

s5, at the film sealing station 7, firstly, receiving the liquid medicine carrier 1 sent by the filling station 6; then, the upper port of the liquid medicine carrier 1 is sealed by a film sealing device 13;

s6, in the label station 8, firstly, the liquid medicine carrier 1 sent by the film sealing station 7 is received; then, labeling is performed on the envelope film by a labeling device 14;

s7, at the output station 9, firstly, the liquid medicine carrier 1 sent in by the label station 8 is received; then, the chemical liquid carrier 1 filled with the chemical liquid is discharged.

The method for storing a liquid medicine of the present embodiment includes step S1, which includes the following steps;

s1.1, pre-storing stacked liquid medicine carriers 1 in the falling guide part 18 and lifting two sides of the lowest layer by falling toggle blades 28;

s1.2, firstly, at the position of the falling process gap 19, the falling crankshaft part 21 drives the falling U-shaped pushing frame 24 to linearly reciprocate in the falling telescopic guide part 23 through the falling connecting rod part 22; then, the falling wedge 25 enters a neutral position between the bottommost layer and the edge of the liquid medicine carrier 1 on the lower second layer, the upper top of the liquid medicine carrier 1 on the lower second layer is separated from the liquid medicine carrier 1 on the bottommost layer by utilizing the inclination, and meanwhile, the falling wedge stirs the blade 28 to swing downwards, so that the liquid medicine carrier 1 on the bottommost layer falls into the conveying mould 16;

s1.3, circulating the falling toggle blade 28, continuously coming to the bottom layer of the liquid medicine carrier 1, and simultaneously, retreating the falling wedge 25 to leave the neutral position, so that the stacked liquid medicine carriers 1 fall onto the falling toggle blade 28 to be supported;

s1.4, the station conveying device 2 drives the conveying clamping fixture 16 loaded with the liquid medicine carrier 1 to leave the falling station 3.

The method for storing a liquid medicine of the present embodiment includes step S5, which includes the following steps;

s5.1, the film sealing discharging reel 32 rotates to drive the film sealing strip 33 output by the film sealing feeding roll 30 to pass through the position above the film sealing station 7 and then be wound on the film sealing discharging reel 32;

s5.2, the filled liquid medicine carrier 1 comes to a film sealing station 7;

s5.3, the first film sealing swing arm 34 and the second film sealing swing arm 35 are in driving linkage, so that the film sealing die-cutting knife 37 and the film sealing thermoplastic welding head 38 do downward overturning movement;

s5.4, die cutting is carried out on the film sealing strip 33 on the liquid medicine carrier 1 by the film sealing die-cutting knife 37, and the film sealing thermoplastic welding head 38 welds the film sealing strip 33 on the edge of the liquid medicine carrier 1 for sealing;

s5.5, turning the film sealing die-cutting knife 37 and the film sealing thermoplastic welding head 38 upwards to leave the film sealing station 7;

s5.6, the filled liquid medicine carrier 1 leaves a film sealing station 7; the film sealing discharging reel 32 rotates to continuously drive the film sealing strip 33 output by the film sealing feeding roll 30 to pass through the position above the film sealing station 7 and then be wound on the film sealing discharging reel 32.

The method for storing a liquid medicine of the present embodiment includes step S6, which includes the following steps;

s6.1, the station conveying device 2 drives the conveying clamping fixture 16 loaded with the liquid medicine carrier 1 to move to a label station 8;

s6.2, the label inking brush 45 inks the label printing head 41 at the position where the label printing head 41 faces upwards to be inked;

s6.3, the first label swing arm 39 and the second label swing arm 42 swing, and the label printing head 41 prints characters downwards on the sealing film of the liquid medicine carrier 1; the label inking brush 45 dips the ink at the label cartridge 46;

s6.4, swinging the first label swing arm 39 and the second label swing arm 42 to enable the label printing head 41 to move upwards;

s6.5, the station conveying device 2 drives the conveying clamping fixture 16 loaded with the liquid medicine carrier 1 to leave the label station 8.

The invention realizes that a liquid medicine carrier 1 (preferably plastic) stores Chinese patent medicine liquid or western medicine and other medicaments, is stable to place and is not easy to turn on one side, and the station conveying device 2 can be a conveying belt and other general structures, and comprises a falling station 3, an adjusting station 4, a cleaning station 5, a filling station 6, a film sealing station 7, a label station 8 and an output station 9 which are protection stations of the invention and do not represent that other stations can not exist or the stations of the invention are combined.

According to the invention, each station is protected independently and in combination. The reasonable combination and disassembly are the protection scope of the invention. The liquid medicine carrier 1 comprises a box body for storing liquid medicine so as to be flat; the outer side wall of the box body is provided with a taper so as to be convenient for stacking and storing empty boxes, and the box body is provided with an upper end edge so as to be convenient for sealing a film;

the falling device 10 utilizes the taper and the edge of the box body to generate a gap so as to be convenient for separation, the ozone blowing pipe 11 realizes non-contact sterilization, the filling machine 12 realizes the filling of liquid medicine, the film sealing device 13 realizes the continuous feeding, the automatic die cutting and welding sealing, and the labeling device 14 realizes the automatic marking. Through the connecting rod structure, automatic inking, quick inking and label printing are realized. It is through coordinated control, simplifies supporting supervisory equipment such as sensor, simplifies the structure, and is durable.

Claims

1. A scutellaria baicalensis test method based on hypertensive mice is characterized by comprising the following steps: comprises the following steps;

s1, preparing the experimental animal as a spontaneous hypertensive rat, 10 Wister rats and a cleaning level; experimental medications, scutellaria baicalensis and benazepril; a kit; the device comprises an experimental instrument, a water bath constant temperature oscillator, a vortex mixer, a centrifuge, a thermostat, an enzyme-labeling instrument, an electronic analysis balance, an animal dissection instrument and an experimental bench;

s2, experimental procedure:

s3, measurement of blood pressure and heart rate:

S4, determination of collagen content in myocardial tissue:

s6, statistical methods;

S7, comparing the results with the change of the blood pressure and the heart rate before and after the rat experiment.

2. A medical fluid storage device characterized by: used for packaging the liquid medicine in the liquid medicine carrier (1); the device comprises a station conveying device (2); the station conveying device (2) comprises a conveying line (15) distributed with conveying clamping tools (16); the transfer mould (16) is a hollow structure, and the upper port of the transfer mould is provided with a mould process notch (17);

distributed on the conveyor line (15):

a drop station (3) having a drop device (10) for placing the liquid medicine carrier (1) onto a transfer mould (16);

an adjusting station (4) provided with a pressing manipulator; pressing down the manipulator to place the liquid medicine carrier (1) with edges and an opening at the upper end in a conveying mould (16);

a cleaning station (5) which is provided with an ozone blowing pipe (11) and is used for introducing ozone gas into the liquid medicine carrier (1) for disinfection;

a filling station (6) having a filling machine (12) for filling the liquid medicament into the liquid medicament carrier (1);

the film sealing station (7) is provided with a film sealing device (13) and is used for sealing the upper port of the liquid medicine carrier (1);

a labelling station (8) having labelling means (14) for labelling the envelope for discrimination;

and/or an output station (9) for outputting the liquid medicine carrier (1) fully loaded with the liquid medicine.

3. A medical fluid storage device characterized by: comprising a falling device (10) located at a falling station (3); the device comprises a falling guide part (18) vertically arranged right above a falling station (3) and a falling stop lever (20) arranged below the falling guide part (18); a drop process gap (19) is arranged between the lower part of the drop guide part (18) and the upper part of the drop stop lever (20); the liquid medicine carrier (1) is stacked in the falling guide part (18);

a falling crankshaft part (21) is arranged on one horizontal side of the falling process gap (19); the falling crankshaft part (21) is hinged with a falling U-shaped pushing frame (24) through a falling connecting rod part (22); a guide rod of the falling U-shaped pushing frame (24) linearly reciprocates in the falling telescopic guide part (23);

a falling & gt wedge (25) is arranged on a fork head of the falling U-shaped pushing frame (24), and the falling & gt wedge (25) is used for entering and exiting a neutral position between the edges of the liquid medicine carriers (1) at the stacking bottom at the falling process gap (19);

a falling speed distribution part (27) is in transmission connection with the falling crankshaft part (21) through a falling bevel gear set (26), a falling stirring blade (28) is in transmission connection with the falling speed distribution part (27), and a falling stirring process gap (29) matched with the liquid medicine carrier (1) is arranged on the falling stirring blade (28);

the falling toggle blade (28) is inserted into a neutral position between the edges of the liquid medicine carriers (1) at the bottom of the stack.

4. The medical fluid storage device according to claim 3, wherein: the liquid medicine carrier (1) comprises a box body for storing liquid medicine so as to be flat; the outer side wall of the box body is provided with a taper so as to be convenient for stacking and storing empty boxes, and the box body is provided with an upper end edge so as to be convenient for sealing a film;

the falling poking blade (28) holds the edge of the bottommost liquid medicine carrier (1) of the liquid medicine carrier (1);

at the position of the falling process gap (19), the falling crankshaft part (21) drives the falling U-shaped pushing frame (24) to reciprocate linearly in the falling telescopic guide part (23) through the falling connecting rod part (22), so that the falling wedge (25) enters and exits the neutral position between the bottommost layer and the edge of the liquid medicine carrier (1) of the lower second layer, the upper top of the liquid medicine carrier (1) of the lower second layer is separated from the liquid medicine carrier (1) of the bottommost layer by utilizing the inclination, and meanwhile, the falling toggle blade (28) swings downwards to enable the liquid medicine carrier (1) of the bottommost layer to fall;

the falling toggle blade (28) circulates and continues to reach the lower part of the medicine liquid carrier (1) at the bottommost layer, and meanwhile, the falling wedge (25) retreats to leave the neutral position, so that the stacked medicine liquid carriers (1) fall onto the falling toggle blade (28).

5. A medical fluid storage device characterized by: the device comprises a film sealing device (13) which comprises a film sealing feeding roll (30) and a film sealing discharging reel (32) which are positioned at the two transverse sides above a film sealing station (7); the film sealing feeding roll (30) conveys a film sealing strip (33) to the film sealing damping counterweight roll group (31), and the film sealing strip (33) passes through the upper part of the liquid medicine carrier (1) positioned at the film sealing station (7); a film sealing damping counterweight roller set (31) is matched on the film sealing strip (33);

a first film sealing swing arm (34) and a second film sealing swing arm (35) are arranged on the side of the film sealing station (7), and the roots of the first film sealing swing arm and the second film sealing swing arm are respectively hinged to a rack of the film sealing device (13);

a film sealing linkage swing arm (36) is hinged between the cantilever ends of the first film sealing swing arm (34) and the second film sealing swing arm (35), and a film sealing die cutting knife (37) and a film sealing thermoplastic welding head (38) are arranged at the end part of the film sealing linkage swing arm (36);

when the film sealing die cutter (37) and the film sealing thermoplastic welding head (38) are downward, the first film sealing swing arm (34) and the second film sealing swing arm (35) are X-shaped; when the film sealing die-cutting knife (37) and the film sealing thermoplastic welding head (38) are upward, the film sealing first swing arm (34), the film sealing second swing arm (35), the film sealing linkage swing arm (36) and the rack form a quadrilateral structure;

the film sealing upper material roll (30) conveys a film sealing strip (33) for sealing the film to a film sealing discharge reel (32) after passing through a film sealing damping counterweight roller set (31) and winds the die-cut film sealing strip (33);

the film sealing first swing arm (34) is driven and the film sealing second swing arm (35) is linked or the film sealing first swing arm (34) is linked and the film sealing second swing arm (35) is driven, the film sealing die cutting knife (37) is used for die cutting the film sealing strip (33), and the film sealing thermoplastic welding head (38) is used for thermoplastic molding of the die-cut film sealing to the edge of the liquid medicine carrier (1).

6. A medical fluid storage device characterized by: the label machine comprises a label device (14), wherein the label device (14) comprises a first label swing arm (39) which is positioned above a label station (8) and is hinged to a rack of the label device (14) at the root part; a label linkage arm (40) is hinged to the upper end of the first label swing arm (39), and a label printing head (41) for printing on a sealing film of the liquid medicine carrier (1) is arranged at the lower end of the label linkage arm (40);

the middle part of the label linkage arm (40) is hinged with the head end part of a second label swing arm (42), and the middle part of the second label swing arm (42) is hinged on the rack;

when the label printing head (41) prints downwards, the first label swing arm (39) and the second label swing arm (42) are in an X-shaped structure, and when the label printing head (41) is upward to be inked, the first label swing arm (39) is positioned on the front side of the second label swing arm (42) and forms a quadrilateral structure together with the label linkage arm (40) and the rack;

the tail end of the second label swing arm (42) is hinged with a label driven connecting rod (43), the label driven connecting rod (43) is connected with a label lifting rod (44), one side of the label lifting rod (44) is provided with a label ink box (46), the label lifting rod (44) is provided with a label inking brush (45), when the label printing head (41) prints downwards, the label ink box (46) is used for dipping ink, and when the label printing head (41) is upwards to be inked, the label printing head (41) which is overturned upwards is used for inking.

7. A method for storing a liquid medicine, characterized by: used for respectively filling the required scutellaria baicalensis medicinal liquid or benazepril in the medicinal liquid carrier (1); by means of a station conveyor (2); the method comprises the following steps of;

s1, placing the liquid medicine carrier (1) on a conveying clamping fixture (16) through a dropping device (10) at a dropping station (3);

s2, at the adjusting station (4), firstly, receiving the liquid medicine carrier (1) sent by the falling station (3); then, the liquid medicine carrier (1) with edges and an opening at the upper end is flatly placed in a conveying mould (16) through a downward pressing manipulator;

s3, in the cleaning station (5), firstly, the liquid medicine carrier (1) sent by the adjusting station (4) is received; then, ozone gas is introduced into the liquid medicine carrier (1) through an ozone blowing pipe (11) for disinfection;

s4, in the filling station (6), firstly, receiving the liquid medicine carrier (1) sent by the cleaning station (5); then, filling the liquid medicine into the liquid medicine carrier (1) through a filling machine (12);

s5, in the film sealing station (7), firstly, receiving the liquid medicine carrier (1) sent by the filling station (6); then, sealing the upper port of the liquid medicine carrier (1) by a film sealing device (13);

s6, in the label station (8), firstly, receiving the liquid medicine carrier (1) sent by the film sealing station (7); then, labeling is carried out on the sealing film through a labeling device (14);

s7, in the output station (9), firstly, the liquid medicine carrier (1) sent by the label station (8) is received; then, the chemical liquid carrier (1) full of the chemical liquid is discharged.

8. A method for storing a liquid medicine, characterized by: the method comprises the following steps of S1;

s1.1, pre-storing the stacked liquid medicine carriers (1) in a falling guide part (18) and lifting two sides of the lowest layer by falling toggle blades (28);

s1.2, firstly, at a falling process gap (19), a falling crankshaft part (21) drives a falling U-shaped pushing frame (24) to linearly reciprocate in a falling telescopic guide part (23) through a falling connecting rod part (22); then, the falling wedge (25) enters a neutral position between the edges of the liquid medicine carriers (1) on the bottommost layer and the second lower layer, the liquid medicine carriers (1) on the second lower layer are separated from the liquid medicine carriers (1) on the bottommost layer by utilizing the inclination, and meanwhile, the falling wedge swings downwards by stirring a blade (28), so that the liquid medicine carriers (1) on the bottommost layer fall into a conveying mould (16);

s1.3, circulating the falling toggle blade (28), continuously coming to the lower part of the liquid medicine carrier (1) at the bottommost layer, and simultaneously, retreating the falling wedge (25) to leave the neutral position, so that the stacked liquid medicine carriers (1) fall onto the falling toggle blade (28) to be supported;

s1.4, the station conveying device (2) drives the conveying clamping fixture (16) loaded with the liquid medicine carrier (1) to leave the falling station (3).

9. A method for storing a liquid medicine, characterized by: the method comprises the following steps of S5;

s5.1, the film sealing discharging reel (32) rotates to drive a film sealing strip (33) output by the film sealing feeding roll (30) to pass through the position above the film sealing station (7) and then be wound on the film sealing discharging reel (32);

s5.2, the filled liquid medicine carrier (1) comes to a film sealing station (7);

s5.3, the first film sealing swing arm (34) and the second film sealing swing arm (35) are in driving linkage, so that the film sealing die cutting knife (37) and the film sealing thermoplastic welding head (38) downwards perform overturning movement;

s5.4, die cutting is carried out on the film sealing strip (33) on the liquid medicine carrier (1) by the film sealing die cutting knife (37), and the film sealing strip (33) is welded on the edge of the liquid medicine carrier (1) by a film sealing thermoplastic welding head (38) for sealing;

s5.5, the film sealing die-cutting knife (37) and the film sealing thermoplastic welding head (38) move upwards to turn over and leave the film sealing station (7);

s5.6, the filled liquid medicine carrier (1) leaves a film sealing station (7); the film sealing discharging reel (32) rotates to continuously drive the film sealing strip (33) output by the film sealing feeding coil (30) to pass through the position above the film sealing station (7) and then be wound on the film sealing discharging reel (32).

10. A method for storing a liquid medicine, characterized by: the method comprises the following steps of S6;

s6.1, the station conveying device (2) drives a conveying clamping fixture (16) loaded with the liquid medicine carrier (1) to move to a label station (8);

s6.2, the label inking brush (45) inks the label printing head (41) at the position where the label printing head (41) faces upwards to be inked;

s6.3, the first label swing arm (39) and the second label swing arm (42) swing, and the label printing head (41) prints characters downwards on the sealing film of the liquid medicine carrier (1) at the same time; a label inking brush (45) dips ink at the label cartridge (46);

s6.4, swinging the first label swing arm (39) and the second label swing arm (42) to enable the label printing head (41) to move upwards;

s6.5, the station conveying device (2) drives the conveying clamping fixture (16) loaded with the liquid medicine carrier (1) to leave the label station (8).