CN112618408B

CN112618408B - Composite protein hair restoration composition and application thereof

Info

Publication number: CN112618408B
Application number: CN202110046514.0A
Authority: CN
Inventors: 郑晓梅
Original assignee: Yang Makeup Source Shanghai Network Technology Co ltd
Current assignee: Yang Makeup Source Shanghai Network Technology Co ltd
Priority date: 2021-01-14
Filing date: 2021-01-14
Publication date: 2023-05-05
Anticipated expiration: 2041-01-14
Also published as: CN112618408A

Abstract

The invention relates to the technical field of hair care, in particular to a composite protein hair care composition and application thereof. It comprises hydrolyzed keratin, hydrolyzed fibroin, and hydrolyzed collagen. The invention creatively prepares the hydrolyzed keratin, hydrolyzed silk and hydrolyzed collagen into a composition, and the three proteins can act synergistically, so that the problem of hair damage is solved from the protein level. The composition can be widely applied to hair-washing and hair-care products in the cosmetic field, and can effectively improve the hair-repairing effect of the hair-washing and hair-care products. The hydrolyzed keratin can repair damaged keratin in the hair scales, smooth the hair scales and improve the hydrophobicity of the hair, and meanwhile, the glossiness of the hair is increased; the hydrolyzed fibroin and the hydrolyzed collagen can deeply penetrate into the hair, so that the hair marrow inside the hair is filled and repaired, and the strength, the elasticity and the toughness of the hair are improved.

Description

Composite protein hair restoration composition and application thereof

Technical Field

The invention relates to the technical field of hair care, in particular to a composite protein hair care composition and application thereof.

Background art

For modern society people, the effect of hair is not limited to physiological functions, and is more reflected in the beauty effect and social effect, and meanwhile, the hair is also a human personality display mode to a certain extent. Therefore, dyeing and perming are increasingly becoming a part of people's daily lives. However, this hair dressing process can cause serious and profound damage to the hair. Meanwhile, due to the development of industrialization in the modern society, environmental pollution and reduction of air quality are silently accelerating the damage degree of hair.

Damage to hair can be broadly classified into the following types according to factors that cause the damage to the hair: physical damage, chemical damage, thermal damage, solar damage, and weathering. Wherein, physical damage refers to damage to hair caused by external force, and one of external force causing hair damage is traction force brought by a comb and friction force caused by comb teeth when combing hair. Chemical damage refers to damage caused by a change in the keratin structure constituting hair due to a chemical reaction occurring in the hair, and substances that can cause a chemical reaction include permanent waving agents, hair dyes, bleaching agents, and the like. The heat damage refers to hair damage caused by too high temperature during blowing or ironing. Sunlight damage and weathering refer to the phenomenon that ultraviolet radiation in sunlight can also cause structural change and photodegradation of hair, and melanin can fade due to oxidation after the hair is irradiated by ultraviolet radiation. Hair is composed of sulfur-containing keratin cells, accounting for about 85-90% of the total hair. Therefore, the nature of hair damage is a change in physicochemical properties such as reduced moisture content of hair, loss of protein degradation, etc. due to natural and human factors. These changes lead to hair that becomes dry and forked, is subject to breakage, rough in hand feel, and reduced in glossiness. In a word, the damage of the hair can seriously affect the beauty, bring trouble to the life of people, and even directly affect the psychological health condition of people.

Commercially available hair restoration products generally consist of silicone oil which spreads on the hair surface by means of a low surface tension and cations which are electrostatically adsorbed on the hair surface. The hair slipping feeling can be effectively improved by using the hair slipping agent in a short time, but accumulation can be caused for a long time, so that the hair becomes heavier and heavier, and the hair state can be worsened and becomes brittle over time. Therefore, the commercial hair care products only can treat the symptoms and cannot treat the root cause of the damaged hair.

Disclosure of Invention

Aiming at the problems, the invention starts from the origin of hair repair and starts from the deep hair damage mechanism, and aims to repair damaged hair quality by using the synergistic effect of three proteins and deep to treat both symptoms and root causes and restore healthy and bright hair.

Specifically, the invention creatively prepares the hydrolyzed keratin, hydrolyzed silk and hydrolyzed collagen into a composition, and the three proteins can act synergistically, so that the problem of hair damage is solved from the aspect of protein. The composition can be widely applied to hair-washing and hair-care products in the cosmetic field, and can effectively improve the hair-repairing effect of the hair-washing and hair-care products.

In a first aspect the present invention provides a composite protein hair restoration composition comprising hydrolyzed keratin, hydrolyzed fibroin, hydrolyzed collagen.

As a preferable technical scheme, the weight ratio of the hydrolyzed keratin to the hydrolyzed silk protein is (1:5): (5:1).

As a preferable technical scheme, the weight ratio of the hydrolyzed fibroin to the hydrolyzed collagen is (1:5): (5:1).

As a preferable technical scheme, the hydrolyzed keratin is hydrolyzed keratin which is hydrolyzed by weak base enzymolysis.

As a preferred embodiment, the molecular weight of the hydrolyzed collagen is not greater than 5000 daltons.

As a preferred embodiment, the hydrolyzed collagen has a molecular weight of no more than 2000 daltons.

As a preferable technical scheme, the molecular weight of the hydrolyzed fibroin is 5000-50000 daltons.

As a preferable technical scheme, the molecular weight of the hydrolyzed fibroin is 10000-20000 daltons.

In a second aspect the present invention provides the use of a composite protein hair restoration composition as described above for hair restoration products including shampoos, conditioners, hair films, hair essential oils and the like.

In a third aspect, the invention provides a shampoo, which is prepared from the composite protein hair restoration composition, and the content of the composite protein hair restoration composition is 0.1-15 wt%.

The beneficial effects are that: the invention creatively prepares the hydrolyzed keratin, hydrolyzed silk and hydrolyzed collagen into a composition, and the three proteins can act synergistically, so that the problem of hair damage is solved from the protein level. The composition can be widely applied to hair-washing and hair-care products in the cosmetic field, and can effectively improve the hair-repairing effect of the hair-washing and hair-care products. The hydrolyzed keratin can repair damaged keratin in the hair scales, smooth the hair scales and improve the hydrophobicity of the hair, and meanwhile, the glossiness of the hair is increased; the molecular weight of the hydrolyzed fibroin and hydrolyzed collagen is very small, and the low molecular weight and high hydrophilicity can enable the hydrolyzed fibroin and hydrolyzed collagen to well permeate into hair, and the hair marrow inside the hair is filled and repaired. All three hydrolyzed proteins contain rich amino acids, and have good affinity and biocompatibility with the amino acids in the hairline, so that the amino acids in the hairline can be effectively affinitized and repaired, and the health degree, strength, elasticity and toughness of the hairline are improved.

The function of protein in conditioning is that the structural composition and molecular mass of the protein are closely related, and the key is that the protein forms hydrogen bonds with keratin in hair, and water-soluble protein has certain repairing effect on damaged hair. This is due to Mao Xiaopi damage to damaged hair, which exposes the proteins, increases the hydrophilicity of the hair, facilitates water-soluble protein binding, forms a protein film, and allows the hair to be easily combed and shiny.

Drawings

FIG. 1 is a scanning electron microscope image (SEM image) of a hair strand before it has been treated with the composition.

Fig. 2 is a scanning electron microscope image (SEM image) of hair strands treated with the composition of example 1.

Detailed Description

The technical features of the technical solution provided in the present invention will be further clearly and completely described in connection with the detailed description below, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

The words "preferred," "more preferred," and the like in the present disclosure refer to embodiments of the present disclosure that may provide certain benefits in some instances. However, other embodiments may be preferred under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.

It should be understood that all numbers expressing, for example, amounts of ingredients used in the specification and claims, except in any operating example or otherwise indicated, are to be understood as being modified in all instances by the term "about".

The hydrolyzed keratin is hydrolyzed liquid obtained by hydrolyzing keratin, the keratin is one of fiber structure protein family, and keratin peptide chain is multi-domain peptide chain and is divided into rod-shaped middle area and terminal peptide area with high retention. The peptide chain of the rod domain has a periodic sequence structure of 8 peptide repeats. All intermediate filamins have a similar peptide chain structure. Keratin peptide chains are typically alpha helices. The heterologous complex helix formed by the combination of acidic and basic keratin peptide chains is a characteristic conformational form of keratin. Keratin is the major protein that forms the outer layers of hair, corners, paws, and human skin. Keratin can protect epithelial tissue cells from injury or stress. The hydrolyzed keratins of the present invention may be obtained commercially or may be prepared according to conventional means in the art.

Because of the specific microstructure of keratin, it is generally insoluble in water, dilute acids or bases, and requires strong acids such as sulfuric acid, hydrochloric acid, etc., or strong bases such as sodium hydroxide, etc., to hydrolyze keratin to produce hydrolyzed keratin. However, the structure of tryptophan, amino acids including hydroxyl, mercapto, guanidino, etc. in keratin is destroyed in the strong acid and alkali environments, which affects the function of the composition.

In some preferred embodiments, the hydrolyzed keratin is hydrolyzed keratin that has been enzymatically hydrolyzed using a weak base.

In some embodiments, the method of preparing hydrolyzed keratin using weak base enzymatic hydrolysis comprises the steps of:

(1) Cleaning and drying keratin-containing raw materials, controlling the water content to be below 2%, and crushing the dried raw materials to obtain granular powder;

(2) Adding the granular powder obtained in the step (1) into water, regulating the pH of the solution to 8.5-9.5 by using food-grade alkali, and boiling for 8-12h to obtain an alkali treatment solution;

(3) Adding the alkali treatment solution obtained in the step (2) into Protamex protease and Flavourzyme enzyme for enzymolysis, inactivating enzyme after the enzymolysis is finished, and centrifuging or filtering to obtain clear liquid;

(4) Concentrating the clear liquid obtained in the step (3) in vacuum, wherein the concentration of the solution is controlled to be more than 40%;

(5) And (3) spray drying the concentrated solution obtained in the step (4) to obtain hydrolyzed keratin.

The hydrolyzed keratin is rich in a plurality of amino acids such as tryptophan, cysteine, methionine, proline and the like, can permeate into hair fibers from damaged and rolled hair scales, and interacts with components in structures such as fur, medulla and the like in the hair fibers, so that the hair protective film on the surface of the hair fibers can be repaired, and hair damage caused by rolling, falling-off and the like of the hair protective film is avoided.

The hydrolyzed fibroin is hydrolyzed liquid obtained after the fibroin is hydrolyzed. The source of the hydrolyzed fibroin is not particularly limited, and the hydrolyzed fibroin can be purchased from the market or obtained by hydrolysis by a conventional means.

In some embodiments, the method of preparing hydrolyzed fibroin comprises the steps of:

(1) Adding a proper amount of deionized water into the silkworm cocoons subjected to high-temperature sterilization in a reaction kettle, and heating to a certain temperature;

(2) Adding a proper amount of pH regulator into the system to regulate the pH value, and preserving heat and hydrolyzing for 1-5 hours;

(3) Cooling the above system to room temperature, decolorizing, filtering, and removing residue to obtain fibroin hydrolysate (hydrolyzed fibroin).

In some preferred embodiments, step (2) requires a gradient to adjust the ph of the system; preferably, the gradient system for adjusting the pH value comprises the following steps:

s01: adding an alkaline pH value regulator into the system, regulating the pH value of the system to at least 10.0, and then stirring and hydrolyzing for 30-45 min;

s02: deionized water and an acidic pH value regulator are added into the system, the pH value of the system is regulated to 9.0-10.0, and the system is stirred and hydrolyzed for 45-60 min;

s03: deionized water and an acidic pH value regulator are added into the system, the pH value of the system is regulated to 8.5-9.0, and the system is stirred and hydrolyzed for 1-3 hours.

In some preferred embodiments, the heating temperature in step (1) is 45-60 degrees celsius; preferably, the heating temperature is 50-55 ℃.

In some preferred embodiments, the hydrolyzed fibroin has a molecular weight of 5000 to 50000 daltons.

Further preferably, the molecular weight of the hydrolyzed fibroin is 10000-20000 daltons.

The applicant finds that the unordered entangled protein bundles in the cocoons can be carded, disentangled and unbundled through the method, the unbundled protein bundles are hydrolyzed into peptide chains with different molecular weights under a specific alkaline pH value environment, the fibroin is rapidly disentangled under a higher pH value condition by regulating and controlling the pH value of a system in a gradient regulating mode, so that the components such as water, pH value and the like in the system can enter into a region where the fibroin is tightly accumulated. And then further reducing the pH value of the system to form protein bundles with higher randomness, and simultaneously hydrolyzing the disentangled protein bundles in a lower pH value environment to generate peptide chains with certain size. And then further reducing the pH value of the system, further hydrolyzing the peptide chain generated by hydrolysis, and adjusting the molecular weight of the peptide chain. On the one hand, the pH value is too high, the alkaline solution in the system cannot enter the crystallization area of the fibroin in a short time, and the protein bundles in the amorphous area are excessively hydrolyzed to form peptide chains with smaller molecular weight under the high alkaline environment, even form single amino acid, so that the effect of the fibroin in the application is influenced. The applicant finds that by adopting a specific pH value adjusting mode, the hydrolysis of the fibroin can be more sufficient, and the molecular weight of the peptide chain obtained by the hydrolysis can be fully regulated and controlled, so that the hydrolyzed fibroin with excellent performance is obtained.

The hydrolyzed collagen protein is protein peptide obtained by hydrolyzing collagen protein. The relative molecular weight of the collagen is 30 ten thousand daltons (D), and the collagen is a triple-helical structure formed by combining 3 peptide chains and intertwined, is directly applied, is difficult to attach to damaged hair, reduces the roughness of the surface of the hair, and cannot be directly absorbed by the damaged hair, so that the collagen needs to be hydrolyzed to obtain hydrolyzed collagen with smaller molecular weight.

The source of the hydrolyzed collagen in the present invention is not particularly limited, and it may be commercially available, or may be prepared by a conventional method, for example, a collagen peptide obtained by subjecting modified collagen such as collagen or gelatin to hydrolysis treatment with an enzyme, an acid, an alkali or the like, or an artificially synthesized collagen peptide. As the collagen and gelatin, for example, collagen and gelatin derived from animals such as cattle, pigs and chickens or from fish, and particularly collagen proteins extracted from connective tissues such as skin, bones and tendons of animals, fish skin and scales can be used. In the present invention, collagen obtained from fish skin, fish heart artery, etc. is preferably used.

In some embodiments, the hydrolyzed collagen has a molecular weight of no greater than 5000 daltons.

Further preferably, the hydrolyzed collagen has a molecular weight of no greater than 2000 daltons.

Further preferably, the hydrolyzed collagen contains an appropriate amount of chondroitin sulfate. Chondroitin sulfate in the present invention is a type of glycosaminoglycan covalently linked to a protein to form a proteoglycan, consisting of alternating glucuronic acid and N-acetylgalactosamine (also known as N-acetylgalactosamine) disaccharide units linked to serine residues of the core protein through a sugar-like linking region.

The applicant has found that the hair restoration effect of the composition can be further improved by controlling the molecular weight of the hydrolyzed collagen, the molecular weight of the hydrolyzed fibroin, and the like. The applicant speculates that the adhesive film can be formed on the surface of the damaged hair after the three components with specific molecular weights and proportions are compounded, so that the permeation of the small molecular weight hydrolyzed collagen Wang Fasi in the composition is promoted, the repair of epidermal cells to be stripped on the surface of the hair is promoted, meanwhile, the components such as hydrolyzed fibroin and hydrolyzed keratin with higher molecular weight are utilized to have stronger adhesive force on the surface of the hair, and a protective film is formed on the surface of the hair under the interaction of the components to repair damaged hair, so that the brittle failure, bifurcation and falling of the hair are reduced, and meanwhile, the antistatic effect is realized, and the hair is smooth.

In some embodiments, the weight ratio of hydrolyzed keratin to hydrolyzed fibroin is (1:5): (5:1).

Further preferably, the weight ratio of the hydrolyzed fibroin to the hydrolyzed collagen is (1:5): (5:1).

Further preferably, the mass ratio of the hydrolyzed keratin, hydrolyzed fibroin and hydrolyzed collagen is 1:1.2:2. The applicant finds that the improvement of the composition on the hair quality can be achieved through a part of improvement by compounding the components in the proportion.

A second aspect of the present invention provides the use of a complex protein hair restoration composition as described above for hair restoration products including, but not limited to, shampoos, conditioners, hair films, hair care essential oils, and the like.

In a third aspect, the invention provides a shampoo, which is prepared from the composite protein hair restoration composition, and the content of the composite protein hair restoration composition is 0.1-15 wt%. In addition, the invention also provides a hair conditioner, the preparation raw materials of which comprise the composite protein hair restoration composition, and the content of the composite protein hair restoration composition is 0.1-15 wt%.

The other components, preparation process and the like of the shampoo and the hair conditioner are not particularly limited, and the shampoo and the hair conditioner can be prepared by adding the components such as the corresponding surfactant and the like into the composition.

The present invention will be specifically described below by way of examples. It is noted herein that the following examples are given solely for the purpose of further illustration and are not to be construed as limitations on the scope of the invention, as will be apparent to those skilled in the art in light of the foregoing disclosure.

Examples

Example 1: example 1 provides a composite protein hair restoration compositionThe weight ratio of the hydrolyzed keratin to the hydrolyzed fibroin to the hydrolyzed collagen is 1:1.2:2. The molecular weight of the hydrolyzed collagen is 2000 daltons, and the molecular weight of the hydrolyzed fibroin is 15000 daltons; the preparation method of the hydrolyzed fibroin comprises the following steps:

(1) Adding a proper amount of deionized water into the silkworm cocoons subjected to high-temperature sterilization in a reaction kettle, and heating to 55 ℃;

(2) Adding a proper amount of pH value regulator into the system to regulate the pH value, wherein the pH value of the gradient regulating system comprises the following steps: s01: adding an alkaline pH value regulator into the system, regulating the pH value of the system to 10.5, and then stirring and hydrolyzing for 40min; s02: adding deionized water and an acidic pH value regulator into the system, regulating the pH value of the system to 9.50, and stirring and hydrolyzing for 50min; s03: deionized water and an acidic pH value regulator are added into the system, the pH value of the system is regulated to 9.0, and the system is stirred and hydrolyzed for 2 hours;

Example 2: example 1 provides a composite protein hair restoration composition comprising hydrolyzed fibroin, hydrolyzed collagen, at a 1.2:2 lifting weight ratio. The molecular weight of the hydrolyzed collagen is 2000 daltons, and the molecular weight of the hydrolyzed fibroin is 15000 daltons; the preparation method of the hydrolyzed fibroin comprises the following steps:

Example 3: example 1 provides a composite protein hair restoration composition comprising hydrolyzed keratin, hydrolyzed collagen, at a weight to weight ratio of 1:2. The molecular weight of the hydrolyzed collagen is 2000 daltons.

Example 4: example 1 provides a composite protein hair restoration composition comprising hydrolyzed keratin and hydrolyzed fibroin in a 1:1.2 basis weight ratio. The molecular weight of the hydrolyzed fibroin is 15000 daltons; the preparation method of the hydrolyzed fibroin comprises the following steps:

Example 5: example 1 provides a composite protein hair restoration composition comprising hydrolyzed keratin, hydrolyzed fibroin, and hydrolyzed collagen, in a weight to weight ratio of 1:1.2:2. The molecular weight of the hydrolyzed collagen is 2000 daltons (which comprises 27wt% chondroitin sulfate, a Peptan series product from ABYSS corporation, france), and the molecular weight of the hydrolyzed fibroin is 15000 daltons; the preparation method of the hydrolyzed fibroin comprises the following steps:

(2) Adding a proper amount of pH value regulator into the system to regulate the pH value, wherein the pH value of the gradient regulating system comprises the following steps: s01: adding an alkaline pH value regulator into the system, regulating the pH value of the system to 10.5, and then stirring and hydrolyzing for 3.5 hours;

Example 6: example 1 provides a composite protein hair restoration composition comprising hydrolyzed keratin, hydrolyzed fibroin, and hydrolyzed collagen, in a weight to weight ratio of 1:1.2:2. The molecular weight of the hydrolyzed collagen is 2000 daltons, and the molecular weight of the hydrolyzed fibroin is 15000 daltons; the preparation method of the hydrolyzed fibroin comprises the following steps:

(2) Adding a proper amount of pH value regulator into the system to regulate the pH value, wherein the pH value of the gradient regulating system comprises the following steps: s01: adding an alkaline pH value regulator into the system, regulating the pH value of the system to 10.5, and then stirring and hydrolyzing for 90min; s02: deionized water and an acidic pH value regulator are added into the system, the pH value of the system is regulated to 9.0, and the system is stirred and hydrolyzed for 2 hours;

Example 7: example 1 provides a composite protein hair restoration composition comprising hydrolyzed keratin, hydrolyzed fibroin, and hydrolyzed collagen, in a weight to weight ratio of 1:1.2:2. The molecular weight of the hydrolyzed collagen is 2000 daltons, and the molecular weight of the hydrolyzed fibroin is 15000 daltons; the preparation method of the hydrolyzed fibroin comprises the following steps:

(2) Adding a proper amount of pH value regulator into the system to regulate the pH value, wherein the pH value of the gradient regulating system comprises the following steps: s01: adding an alkaline pH value regulator into the system, regulating the pH value of the system to 10.5, and then stirring and hydrolyzing for 40min; s02: deionized water and an acidic pH value regulator are added into the system, the pH value of the system is regulated to 9.50, and the system is stirred and hydrolyzed for 3 hours;

Performance testing

1. The applicant selected hair after permanent waving and tested hair surface electron microscopy images of the hair before and after application of the composition of example 1, specifically: immersing the damaged hair in the aqueous solution of the composite protein hair restoration composition of example 1 for 20 minutes, taking out the hair after soaking, naturally airing, and taking SEM pictures again, wherein the results are shown in FIG. 1 and FIG. 2, wherein FIG. 1 is an untreated SEM picture, and FIG. 2 is a treated SEM picture, it can be seen that the hair scales on the hair surface are substantially closed after the hair is treated by the composition of example 1, and the composition has a certain repairing effect on the hair, and will necessarily have a more obvious repairing effect after the hair is treated by the composition of example 1 for a plurality of times.

2. Carding work test: a commercial lady shampoo A was taken and the composition of example (content: 5 wt%) was added thereto to obtain shampoo B _i And shampoo A was used as a control, shampoo B was used as a control _i Shampoo bundle B (5 min). After that, the combing work was measured again after the two hair strands were naturally dried. The smaller the combing work, the easier the hair bundle is to comb, and the better the smoothness of the hair bundle is. The results are shown in Table 2.

TABLE 2

	Original combing work/gf-mm	After treatment carding work/gf-mm
			Example 1	784.23	480.83
Example 2	782.58	579.44
			Example 3	775.80	564.29
Example 4	786.47	591.87
			Example 5	783.52	538.55
Example 6	790.82	533.14
			Example 7	786.34	539.22
Comparative example	796.15	638.30

From the above experimental results, it can be seen that the composition provided by the invention can greatly reduce combing work, i.e. can greatly improve the smoothness of the hair bundle, i.e. the formula has excellent conditioning property on hair. And the effect is best when three components, specific proportions and specific compounded molecular weights are adopted.

3. Contact angle test: a commercial lady hair conditioner C was taken and the composition of example (content: 5 wt%) was added thereto to obtain a hair conditioner D _i The hair tress was cleaned with the same surfactant solution and the same method. Then, hair conditioner C was used as a control, hair conditioner D _i Shampoo bundle D (5 min). The contact angle was measured again after naturally airing the hair bundle. The greater the contact angle, the higher the degree of hydrophobicity of the hair and the better the smoothness of the hair surface. The results are shown in Table 3.

TABLE 3 Table 3

	Original contact angle/degree	Contact angle/degree after treatment
			Example 1	44.57	83.25
Example 2	43.65	74.38
			Example 3	44.09	75.11
Example 4	44.31	72.88
			Example 5	43.70	77.47
Example 6	43.91	79.98
			Example 7	44.12	80.04
Comparative example	43.25	68.35

From the experimental results, the composite protein hair restoration composition provided by the invention can greatly improve the contact angle of hair bundles, the contact angle is greatly improved to 83.25 degrees, 86.78 percent of the contact angle is improved, and the improvement effect is very remarkable, namely, the restoration effect of a hair conditioner formula on hair is improved. Likewise, the effect is best when three components, specific proportions, and specific compounded molecular weights are employed.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the invention in any way, and any person skilled in the art may make modifications or alterations to the disclosed technical content to equivalent embodiments without departing from the technical content of the present invention, and any simple modification, equivalent changes and alterations to the above embodiments according to the technical substance of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A composite protein hair restoration composition, which is characterized by comprising hydrolyzed keratin, hydrolyzed fibroin and hydrolyzed collagen; the weight ratio of the hydrolyzed keratin to the hydrolyzed fibroin to the hydrolyzed collagen is 1:1.2:2; the preparation method of the hydrolyzed fibroin comprises the following steps: (1) Adding a proper amount of deionized water into the silkworm cocoons subjected to high-temperature sterilization in a reaction kettle, and heating to 55 ℃; (2) Adding a proper amount of pH regulator into the system to regulate the pH value, and preserving heat for hydrolysis; (3) Cooling the system to room temperature, decoloring, filtering, and removing filter residues to obtain fibroin hydrolysate, namely the hydrolyzed fibroin; in the step (2), the pH value of the system is required to be adjusted in a gradient manner; the pH value of the gradient regulating system comprises the following steps: s01: adding an alkaline pH value regulator into the system, regulating the pH value of the system to 10.5, and then stirring and hydrolyzing for 40min; s02: adding deionized water and an acidic pH value regulator into the system, regulating the pH value of the system to 9.50, and stirring and hydrolyzing for 50min; s03: deionized water and an acidic pH value regulator are added into the system, the pH value of the system is regulated to 9.0, and the system is stirred and hydrolyzed for 2 hours;

the hydrolyzed keratin is hydrolyzed keratin by weak base enzymolysis;

the molecular weight of the hydrolyzed collagen is not higher than 2000 daltons;

the molecular weight of the hydrolyzed fibroin is 10000-20000 daltons.

2. The use of the composite protein hair restoration composition according to claim 1, wherein the composite protein hair restoration composition is applied to a hair restoration product, and the hair restoration product comprises shampoo, conditioner, hair mask and hair care essential oil.

3. A shampoo characterized in that the preparation raw material comprises the composite protein hair restoration composition according to claim 1, and the content of the composite protein hair restoration composition is 0.1-15 wt%.