CN112616460A - Rapid seedling propagation method for actinidia arguta - Google Patents
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- 244000298800 Actinidia arguta Species 0.000 title claims abstract description 40
- 235000016416 Actinidia arguta Nutrition 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000005520 cutting process Methods 0.000 claims abstract description 111
- 241000196324 Embryophyta Species 0.000 claims abstract description 29
- 230000037431 insertion Effects 0.000 claims abstract description 15
- 238000003780 insertion Methods 0.000 claims abstract description 15
- 239000004744 fabric Substances 0.000 claims abstract description 10
- 238000002791 soaking Methods 0.000 claims abstract description 10
- 230000006698 induction Effects 0.000 claims abstract description 9
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 238000005201 scrubbing Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000002689 soil Substances 0.000 claims description 35
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 230000000249 desinfective effect Effects 0.000 claims description 7
- 230000003020 moisturizing effect Effects 0.000 claims description 7
- 239000012188 paraffin wax Substances 0.000 claims description 7
- 239000002985 plastic film Substances 0.000 claims description 7
- 229920006255 plastic film Polymers 0.000 claims description 7
- 239000012286 potassium permanganate Substances 0.000 claims description 7
- 230000001737 promoting effect Effects 0.000 claims description 7
- 239000004576 sand Substances 0.000 claims description 7
- 238000009395 breeding Methods 0.000 abstract description 5
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 description 5
- 244000298715 Actinidia chinensis Species 0.000 description 2
- 235000009434 Actinidia chinensis Nutrition 0.000 description 2
- 240000007822 Actinidia kolomikta Species 0.000 description 2
- 235000002246 Actinidia kolomikta Nutrition 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000219068 Actinidia Species 0.000 description 1
- 240000006274 Actinidia polygama Species 0.000 description 1
- 235000016413 Actinidia polygama Nutrition 0.000 description 1
- 241000219066 Actinidiaceae Species 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/28—Raised beds; Planting beds; Edging elements for beds, lawn or the like, e.g. tiles
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a rapid seedling propagation method of actinidia arguta, which comprises the following steps: firstly, explant selection: selecting 1-2 year-old robust plants in 11 months per year, selecting branches with stem thickness of 0.5-0.8cm and plump axillary buds on the plants, and cutting the branches into insertion sections with length of 15-20cm, wherein each section is provided with 3-5 buds; step two, pretreatment: placing the insert obtained in the first step in a concentration of 0.05-0.13 mg.L‑1K of2SO4Soaking in the solution for 30-40 min; step three, induction culture: taking out the pretreated cutting section in the second step, covering the cutting section with a wet cloth with the thickness of 3-5mm, culturing in an environment with the temperature of 20-32 ℃ and the humidity of 60-75%, and scrubbing the cutting section with water with the temperature of 25 ℃ for 1 time each morning and evening to obtain the cutting section for rooting and sprouting. The invention has low cost and high efficiencyThe method has the advantages of quick effect, simplicity and strong operability, and can be widely applied to quick large-scale seedling breeding and cultivation of actinidia arguta.
Description
Technical Field
The invention relates to the technical field of actinidia arguta breeding, in particular to a rapid seedling breeding method for actinidia arguta.
Background
Actinidia arguta, also known as Actinidia chinensis planch, Actinidia arguta, Actinidia kolomikta, Aucklandia chinensis, Actinidia kolomikta, Actinidia polygama, Actinidia chinensis, etc., is a vine deciduous plant of Actinidia of Actinidiaceae. The fruit is edible, has high nutritive value, contains a large amount of vitamin C, starch, pectin and the like, and can be processed into jam, fruit juice, preserved fruit, can, wine or used for making cakes, candies and other various foods. Can also be used as medicine, and has effects of strengthening body, relieving fever, invigorating stomach, and stopping bleeding; the root and the root bark have certain treatment and inhibition effects on digestive tract cancer; the oil content of the seeds is 35.62 percent, the actinidia arguta is better dry oil, and actinidia arguta also has good honey source and ornamental plants, so that actinidia arguta has extremely high economic and medicinal values and the like. At present, actinidia arguta is mainly wild and assisted by artificial cultivation (variety).
In the prior art, actinidia arguta is usually propagated in a seed, grafting, tissue culture and other modes, the propagation characters of seeds are separated, and the grafting survival rate is low. The tissue culture has high input cost and poor operability, and variation or mutation may occur during callus induction, callus redifferentiation and subculture proliferation in the tissue culture, and even if seedlings grow, the characteristics of the seedlings are different from those of parents, so that a rapid seedling propagation method of actinidia arguta is needed to be designed, and rapid large-scale seedling propagation and cultivation of actinidia arguta can be realized.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a rapid seedling propagation method for actinidia arguta.
In order to achieve the purpose, the invention adopts the following technical scheme:
a rapid seedling propagation method of actinidia arguta comprises the following steps:
firstly, explant selection: selecting 1-2 year-old robust plants in 11 months per year, selecting branches with stem thickness of 0.5-0.8cm and plump axillary buds on the plants, and cutting the branches into insertion sections with length of 15-20cm, wherein each section is provided with 3-5 buds;
step two, pretreatment: placing the insert obtained in the first step in a concentration of 0.05-0.13 mg.L-1K of2SO4Soaking in the solution for 30-40 min;
step three, induction culture: fishing out the pretreated cutting section in the second step, covering the cutting section with wet cloth with the thickness of 3-5mm, culturing in an environment with the temperature of 20-32 ℃ and the humidity of 60-75%, and scrubbing the cutting section with water with the temperature of 25 ℃ for 1 time each morning and evening to obtain cutting sections for rooting and sprouting;
fourthly, preparing a seedbed: selecting dry soil with high terrain, loose soil texture and certain water retention capacity, ploughing the soil, finishing into a seedbed with the width of 1-1.4m and the height of 23-27cm, mixing a proper amount of fine sand in the bed surface, and disinfecting the soil for 1-2 times by using 0.5-1% of potassium permanganate;
fifthly, cutting: cutting the sprouting cuttings in the third step according to the plant spacing of 10-13cm, obliquely inserting the cuttings into a seedbed according to the angle of 30 degrees, leaving the top with 1 bud and about 2cm long to be exposed outside the soil, covering a plastic film for moisturizing, controlling the temperature to be 17-20 ℃, after cutting, covering a shade shed, covering a 95% sunshade net, when the young shoots grow to 5-7cm, keeping 3-4 leaves for timely pinching, controlling the overground part to grow, promoting the rooting and seedling formation, and obtaining the actinidia arguta seedling after the seedlings grow to 10-15 cm.
Preferably, the upper cutting opening of the middle section in the first step is cut under a flat condition at a distance of 1-2 cm from the bud, the opening is sealed by paraffin, and the lower cutting opening is cut under a 45-degree oblique cutting condition.
Preferably, in the first step, a branch with a stem diameter of 0.6cm and a plump axillary bud on a plant is selected, and an insertion section with a growth of 18cm is cut, wherein each section is provided with 4 buds.
Preferably, K in the second step2SO4The concentration of the solution was 0.08 mg.L-1The soaking time is 35 min.
Preferably, the thickness of the wet cloth in the third step is 3 cm.
Preferably, the temperature in the third step is controlled to be 25 ℃ and the humidity is controlled to be 65%.
Preferably, the width of the seedbed in the fourth step is 1.3m, and the height is 25 cm.
Preferably, in the fifth step, the plant spacing is 12cm, the temperature is controlled at 18 ℃, the length of a new shoot is controlled at 6cm, 3 leaves are left for timely pinching, and the seedling grows to 13cm to obtain the actinidia arguta seedling.
The invention has the beneficial effects that:
1. carry out preliminary treatment to the cutting through the setting, carry out sterilization treatment to the cutting, improve the cutting bacterinertness, and induced cultivation can induce the cutting to sprout rapidly, and then greatly improve seedling speed and make the reinforcing of taking root in later stage.
2. Through setting up quick a large amount of cuttage of seedbed, can grow seedlings fast and breed a large amount of actinidia arguta seedlings in the short-term, guarantee the seedling quality simultaneously, and then improve seedling breeding speed, realize seedling factory ization, large-scale production.
3. According to the invention, the cuttage process is completed according to the operation of the invention, the rooting can be rapidly enhanced, the seedling breeding speed is accelerated, the nutrition accumulation and growth capacity is improved, the seedling stress resistance is enhanced, and the seedling transplanting survival rate in the later period is improved.
The method has the advantages of low cost, high efficiency, rapidness, strong operability and wide application in the rapid large-scale seedling propagation and cultivation of actinidia arguta.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
Example one
A rapid seedling propagation method of actinidia arguta comprises the following steps:
firstly, explant selection: selecting 1-year-old robust plants in 11 months each year, selecting branches with stems of 0.5cm thick and full axillary buds on the plants, cutting into 15 cm-long insertion sections, wherein each section is provided with 3 buds, cutting the upper cutting openings of the insertion sections at a distance of 1cm from the buds by adopting flat cutting, sealing the opening by using paraffin, and cutting the lower cutting openings by beveling at 45 degrees;
step two, pretreatment: the insert obtained in the first step was placed at a concentration of 0.05 mg.L-1K of2SO4Soaking in the solution for 30 min;
step three, induction culture: fishing out the pretreated cutting section in the second step, covering the cutting section with wet cloth with the thickness of 3mm, culturing in an environment with the temperature of 20 ℃ and the humidity of 60%, and scrubbing the cutting section with water with the temperature of 25 ℃ for 1 time each morning and evening to obtain cutting sections for rooting and sprouting;
fourthly, preparing a seedbed: selecting dry soil with high terrain, loose soil quality and certain water retention capacity, ploughing the soil, finishing into a seedbed with the width of 1m and the height of 23cm, mixing a proper amount of fine sand in the bed surface, and disinfecting the soil for 1 time by using 0.5 percent potassium permanganate;
fifthly, cutting: cutting the sprouting cuttings in the third step according to the plant spacing of 10cm, obliquely inserting the cuttings into a seedbed according to the angle of 30 degrees, leaving the top with 1 bud and about 2cm long to be exposed outside the soil, covering a plastic film for moisturizing, controlling the temperature to be 17 ℃, after cutting, covering a shade shed, covering a 95% sunshade net, when the young shoots grow to 5cm, leaving 3 leaves for timely pinching, controlling the overground part to grow, promoting the rooting and seedling formation, and after the seedlings grow to 10cm, obtaining the actinidia arguta seedling.
Example two
A rapid seedling propagation method of actinidia arguta comprises the following steps:
firstly, explant selection: selecting 1-year-old robust plants in 11 months each year, selecting branches with stems of 0.6cm thick and full axillary buds on the plants, cutting the branches into 16 cm-long insertion sections, wherein each section is provided with 4 buds, cutting the upper cutting openings of the insertion sections at a distance of 1cm from the buds by adopting flat cutting, sealing the upper cutting openings by using paraffin, and cutting the lower cutting openings by beveling at 45 degrees;
step two, pretreatment: the insert obtained in the first step was placed at a concentration of 0.06 mg.L-1K of2SO4Soaking in the solution for 32 min;
step three, induction culture: fishing out the pretreated cutting section in the second step, covering the cutting section with wet cloth with the thickness of 4mm, culturing in an environment with the temperature of 22 ℃ and the humidity of 63 percent, and scrubbing the cutting section with water with the temperature of 25 ℃ for 1 time each morning and evening to obtain cutting sections for rooting and sprouting;
fourthly, preparing a seedbed: selecting dry soil with high terrain, loose soil texture and certain water retention capacity, ploughing the soil, finishing into a seedbed with the width of 1.2m and the height of 25cm, mixing a proper amount of fine sand in the bed surface, and disinfecting the soil for 1 time by using 0.6 percent potassium permanganate;
fifthly, cutting: cutting the sprouting cuttings in the third step according to the plant spacing of 11cm, obliquely inserting the cuttings into a seedbed according to the angle of 30 degrees, leaving the top with 1 bud and about 2cm long to be exposed outside the soil, covering a plastic film for moisturizing, controlling the temperature to be 18 ℃, after cutting, covering a shade shed, covering a 95% sunshade net, when the young shoots grow to 6cm, leaving 3 leaves for timely pinching, controlling the overground part to grow, promoting the rooting and seedling formation, and after the seedlings grow to 12cm, obtaining the actinidia arguta seedling.
EXAMPLE III
The method comprises the following steps:
firstly, explant selection: selecting 2-year-old robust plants in 11 months each year, selecting branches with stems of 0.6cm thick and full axillary buds on the plants, cutting the branches into 18 cm-long insertion sections, wherein each section is provided with 4 buds, cutting the upper cutting openings of the insertion sections at a distance of 2cm from the buds by adopting flat cutting, sealing the upper cutting openings by using paraffin, and cutting the lower cutting openings by beveling at 45 degrees;
step two, pretreatment: the insert obtained in the first step was placed at a concentration of 0.08 mg.L-1K of2SO4Soaking in the solution for 35 min;
step three, induction culture: fishing out the pretreated cutting section in the second step, covering the cutting section with wet cloth with the thickness of 3mm, culturing in an environment with the temperature of 25 ℃ and the humidity of 65%, and scrubbing the cutting section with water with the temperature of 25 ℃ for 1 time each morning and evening to obtain cutting sections for rooting and sprouting;
fourthly, preparing a seedbed: selecting dry soil with high terrain, loose soil texture and certain water retention capacity, ploughing the soil, finishing into a seedbed with the width of 1.3m and the height of 25cm, mixing a proper amount of fine sand in the bed surface, and disinfecting the soil for 1 time by using 0.8 percent potassium permanganate;
fifthly, cutting: cutting the sprouting cuttings in the third step according to the plant spacing of 12cm, obliquely inserting the cuttings into a seedbed according to the angle of 30 degrees, leaving the top with 1 bud and about 2cm long to be exposed outside the soil, covering a plastic film for moisturizing, controlling the temperature to be 18 ℃, after cutting, covering a shade shed, covering a 95% sunshade net, when the young shoots grow to 6cm, leaving 3 leaves for timely pinching, controlling the overground part to grow, promoting the rooting and seedling formation, and after the seedlings grow to 13cm, obtaining the actinidia arguta seedling.
Example four
A rapid seedling propagation method of actinidia arguta comprises the following steps:
firstly, explant selection: selecting 2-year-old robust plants in 11 months per year, selecting branches with stems being 0.7cm thick and axillary buds being plump on the plants, cutting the branches into 19 cm-long insertion sections, wherein each section is provided with 4 buds, cutting the upper cutting opening of each insertion section at a position 1.5 cm away from the buds by adopting flat cutting, sealing the upper cutting opening by using paraffin, and cutting the lower cutting opening at an angle of 45 degrees in a beveling manner;
step two, pretreatment: the insert obtained in the first step was placed at a concentration of 0.12 mg.L-1K of2SO4Soaking in the solution for 38 min;
step three, induction culture: fishing out the pretreated cutting section in the second step, covering the cutting section with wet cloth with the thickness of 4mm, culturing in an environment with the temperature of 30 ℃ and the humidity of 70%, and scrubbing the cutting section with water with the temperature of 25 ℃ for 1 time each morning and evening to obtain cutting sections for rooting and sprouting;
fourthly, preparing a seedbed: selecting dry soil with high terrain, loose soil texture and certain water retention capacity, ploughing the soil, finishing into a seedbed with the width of 1.3m and the height of 25cm, mixing a proper amount of fine sand in the bed surface, and disinfecting the soil for 2 times by using 0.8 percent potassium permanganate;
fifthly, cutting: cutting the sprouting cuttings in the third step according to the plant spacing of 12cm, obliquely inserting the cuttings into a seedbed according to the angle of 30 degrees, leaving the top with 1 bud and about 2cm long to be exposed outside the soil, covering a plastic film for moisturizing, controlling the temperature to be 19 ℃, after cutting, covering a shade shed, covering a 95% sunshade net, when the young shoots grow to 6cm, leaving 4 leaves for timely pinching, controlling the overground part to grow, promoting the rooting and seedling formation, and after the seedlings grow to 14cm, obtaining the actinidia arguta seedling.
EXAMPLE five
A rapid seedling propagation method of actinidia arguta comprises the following steps:
firstly, explant selection: selecting 2-year-old robust plants in 11 months each year, selecting branches with stems of 0.8cm thick and full axillary buds on the plants, cutting the branches into 20 cm-long insertion sections, wherein each section is provided with 5 buds, cutting the upper cutting openings of the insertion sections at a distance of 2cm from the buds by adopting flat cutting, sealing the upper cutting openings by using paraffin, and cutting the lower cutting openings by beveling at 45 degrees;
step two, pretreatment: the insert obtained in the first step was placed at a concentration of 0.13 mg.L-1K of2SO4Soaking in the solution for 40 min;
step three, induction culture: fishing out the pretreated cutting section in the second step, covering the cutting section with a wet cloth with the thickness of 5mm, culturing in an environment with the temperature of 32 ℃ and the humidity of 75%, and scrubbing the cutting section with water with the temperature of 25 ℃ for 1 time each morning and evening to obtain cutting sections for rooting and sprouting;
fourthly, preparing a seedbed: selecting dry soil with high terrain, loose soil texture and certain water retention capacity, ploughing the soil, finishing into a seedbed with the width of 1.4m and the height of 27cm, mixing a proper amount of fine sand in the bed surface, and disinfecting the soil for 2 times by using 1% potassium permanganate;
fifthly, cutting: cutting the sprouting cuttings in the third step according to the plant spacing of 13cm, obliquely inserting the cuttings into a seedbed according to the angle of 30 degrees, leaving the top with 1 bud and about 2cm long to be exposed outside the soil, covering a plastic film for moisturizing, controlling the temperature to be 20 ℃, after cutting, covering a shade shed, covering a 95% sunshade net, when the young shoots grow to 7cm, leaving 4 leaves for timely pinching, controlling the overground part to grow, promoting the rooting and seedling formation, and after the seedlings grow to 15cm, obtaining the actinidia arguta seedling.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. A rapid seedling propagation method of actinidia arguta is characterized by comprising the following steps:
firstly, explant selection: selecting 1-2 year-old robust plants in 11 months per year, selecting branches with stem thickness of 0.5-0.8cm and plump axillary buds on the plants, and cutting the branches into insertion sections with length of 15-20cm, wherein each section is provided with 3-5 buds;
step two, pretreatment: placing the insert obtained in the first step in a concentration of 0.05-0.13 mg.L-1K of2SO4Soaking in the solution for 30-40 min;
step three, induction culture: fishing out the pretreated cutting section in the second step, covering the cutting section with wet cloth with the thickness of 3-5mm, culturing in an environment with the temperature of 20-32 ℃ and the humidity of 60-75%, and scrubbing the cutting section with water with the temperature of 25 ℃ for 1 time each morning and evening to obtain cutting sections for rooting and sprouting;
fourthly, preparing a seedbed: selecting dry soil with high terrain, loose soil texture and certain water retention capacity, ploughing the soil, finishing into a seedbed with the width of 1-1.4m and the height of 23-27cm, mixing a proper amount of fine sand in the bed surface, and disinfecting the soil for 1-2 times by using 0.5-1% of potassium permanganate;
fifthly, cutting: cutting the sprouting cuttings in the third step according to the plant spacing of 10-13cm, obliquely inserting the cuttings into a seedbed according to the angle of 30 degrees, leaving the top with 1 bud and about 2cm long to be exposed outside the soil, covering a plastic film for moisturizing, controlling the temperature to be 17-20 ℃, after cutting, covering a shade shed, covering a 95% sunshade net, when the young shoots grow to 5-7cm, keeping 3-4 leaves for timely pinching, controlling the overground part to grow, promoting the rooting and seedling formation, and obtaining the actinidia arguta seedling after the seedlings grow to 10-15 cm.
2. The rapid seedling propagation method of actinidia arguta according to claim 1, wherein the upper cut of the middle section in the first step is cut at a distance of 1-2 cm from the bud by using a flat cutter, the cut is sealed by using paraffin, and the lower cut is cut at an angle of 45 degrees.
3. The propagation method for the fast seedling of actinidia arguta as claimed in claim 1, wherein in the first step, the branch with 0.6cm thick stem and plump axillary bud is selected from the plant, and cut into 18cm long insertion section, each section has 4 buds.
4. The rapid seedling propagation method of actinidia arguta as claimed in claim 1, wherein in the second step K is selected from the group consisting of2SO4The concentration of the solution was 0.08 mg.L-1The soaking time is 35 min.
5. The rapid seedling propagation method of actinidia arguta according to claim 1, wherein the thickness of the wet cloth in the third step is 3 cm.
6. The rapid seedling propagation method of actinidia arguta according to claim 1, wherein in the third step, the temperature is controlled to be 25 ℃ and the humidity is controlled to be 65%.
7. The rapid seedling propagation method of actinidia arguta according to claim 1, wherein in the fourth step, the width of the seedbed is 1.3m, and the height of the seedbed is 25 cm.
8. The rapid seedling propagation method of actinidia arguta according to claim 1, wherein in the fifth step, the plant spacing is 12cm, the temperature is controlled at 18 ℃, the length of a new tip is controlled at 6cm, 3 leaves are left for timely pinching, and the seedling grows to 13cm to obtain actinidia arguta seedling.
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| CN111149701A (en) * | 2020-02-27 | 2020-05-15 | 通化师范学院 | Rapid seedling propagation method for actinidia arguta |
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