CN112592813A - Automatic nucleic acid extraction method - Google Patents
Automatic nucleic acid extraction method Download PDFInfo
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- CN112592813A CN112592813A CN202011481353.XA CN202011481353A CN112592813A CN 112592813 A CN112592813 A CN 112592813A CN 202011481353 A CN202011481353 A CN 202011481353A CN 112592813 A CN112592813 A CN 112592813A
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- hole
- sleeve
- straw
- deep
- pipe
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
Abstract
The invention relates to the technical field of biomedicine and discloses an automatic nucleic acid extraction method which comprises a clamping plate, wherein a drawing plate is fixedly sleeved at the top of the clamping plate, the top of the drawing plate is provided with six stop holes, and the inner diameter of a limiting pipe is larger than the diameter of a suction pipe and is the same as the diameter of a sleeve. Make deformation hole and sleeve downstream time change shape hole can take place the contact with the top of spacing pipe, deformation hole can't continue to take place deformation simultaneously through and the straw between frictional force make sealing device whole and straw synchronous downstream, along with straw and sealing device to the inside income of deep tube, compress in the volume of deep tube, make mentioning in the deep tube discharge and form one-way air current in the straw through the straw, make outside impurity can't enter into the inside of deep tube through the straw, medicament or the sample in having avoided outside volatile gas or other impurity pollution deep tube, the purity of nucleic acid extraction has been improved.
Description
Technical Field
The invention relates to the technical field of biomedicine, in particular to an automatic nucleic acid extraction method.
Background
Nucleic acid extraction is a very common and important step in biological experiments nowadays, operators can put various reagents prepared in advance into a deep-hole box, and the reagents and samples to be processed in the deep-hole box are processed by controlling a magnetic rod and a magnetic rod sleeve through a mechanical system, and the instrument is generally heated before operation, so that the nucleic acid extraction operation can be carried out after the temperature reaches the test requirement.
But current deep-hole box is for convenient operation, often all can adopt the opening design, and this makes at the in-process of heating, and the reagent can be heated and make volatilizing aggravation to deep-hole distance on the deep-hole box is nearer, and volatilizing medicament enters into other deep-holes very easily, thereby pollutes medicament or the sample in the deep-hole, thereby leads to the extraction purity of final nucleic acid to reduce.
Disclosure of Invention
Aiming at the defects of the existing automatic nucleic acid extraction instrument in the background technology in the using process, the invention provides the automatic nucleic acid extraction instrument and the extraction method thereof, which have the advantage of plugging a deep hole on a deep hole box and solve the problem that other reagents or samples are polluted because the reagents in the deep hole box in the original equipment are volatile.
The invention provides the following technical scheme: the utility model provides an automatic appearance that draws of nucleic acid, includes splint, the fixed cover in top of splint has connect and has taken out the board, it is six to take out the top of board and has seted up the quantity that ends the journey hole and end the journey hole, the bottom fixed mounting of splint has the deep tube just the quantity of deep tube is six just, the top of splint is provided with mechanical driving plate, mechanical driving plate's bottom fixed mounting has the sleeve just telescopic quantity is six, telescopic outer surface fixed cup joints has the straw, sealing device has been cup jointed in the inside activity that ends the journey hole.
Preferably, the inner wall of the stop hole is stepped and is matched with the sealing device.
Preferably, the sealing device comprises a sealing sleeve, a support ring is fixedly sleeved at the bottom of the outer surface of the sealing sleeve, the support ring is matched with a stepped structure arranged on the inner wall of the stroke stopping hole, a position, close to the outer side, of the bottom of the support ring is fixedly installed and corresponds to the position of the deep pipe, a one-way through hole is formed in the bottom of the sealing sleeve, a limiting pipe is fixedly installed at the position, located at the top of the one-way through hole, of the bottom of the inner cavity of the sealing sleeve, and a deformation hole is formed in the top.
Preferably, the axis of the suction pipe and the axis of the sealing device are located on the same central line, and the axis of the suction pipe and the axis of the deep pipe are located on the same central line.
Preferably, the top of the sealing sleeve is made of soft rubber.
Preferably, the aperture of the deformation hole is larger than the pipe diameter of the suction pipe and smaller than the diameter of the sleeve, and the inner diameter of the limiting pipe is larger than the diameter of the suction pipe and is the same as the diameter of the sleeve.
Preferably, the one-way through hole has a larger top aperture than a bottom aperture.
The invention has the following beneficial effects:
1. the invention makes the straw firstly pass through the deformation hole and the limit pipe and contact with the one-way through hole at the bottom of the sealing sleeve by the aperture of the deformation hole being larger than the pipe diameter of the straw and the aperture of the deformation hole being smaller than the diameter of the sleeve, makes the straw extrude the one-way through hole and extend into the deep pipe by the top of the sealing sleeve being made of soft rubber, and makes the tail end of the straw and the sleeve connected part contact with the deformation hole at the top of the sealing sleeve and make the deformation hole and the sleeve joint and move downwards as the straw continues to move downwards, because the inner diameter of the limit pipe is larger than the diameter of the straw and is the same as the diameter of the sleeve, the deformation hole and the sleeve move downwards when the deformation hole cannot continue to deform, and makes the sealing device and the straw move downwards synchronously by the friction force between the deformation hole and the straw, along with straw and sealing device to the inside income of deep tube, compress in the volume of deep tube for mention in the deep tube discharges and form unidirectional air current in the straw through the straw, make outside impurity can't enter into the inside of deep tube through the straw, avoided outside volatile gas or other impurity to pollute medicament or sample in the deep tube, improved the purity of nucleic acid extraction.
2. According to the invention, when the suction pipe finishes the extraction operation and is pulled away from the deep pipe through the movement of the sleeve, the sealing device integrally moves synchronously through the friction force of the suction pipe, when the support ring contacts with the sleeve, the sealing device reaches the stroke limit, the suction pipe is pulled away from the inside of the sealing device, when the suction pipe is separated from the one-way through hole, the one-way through hole resets again, and the top aperture of the one-way through hole is larger than the bottom aperture, so that the gas inside the deep pipe is prevented from leaking out, the phenomenon that the volatilized gas in the deep pipe enters the operation box body to cause the pollution of reagents or samples in other deep pipes is avoided, and the probability of the pollution of impurities in the deep pipe is reduced.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic cross-sectional view of the structure of the present invention;
FIG. 3 is an enlarged view of the point A in the structure of FIG. 2.
In the figure: 1. a splint; 2. drawing the plate; 3. a stop hole; 4. deep tube; 5. a mechanical drive plate; 6. a sleeve; 7. a straw; 8. a sealing device; 81. sealing sleeves; 82. a support ring; 83. a positioning ring; 84. passing the holes in one direction; 85. a limiting pipe; 86. a deformation hole is formed.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-3, an automatic nucleic acid extraction apparatus and an extraction method thereof includes a clamp plate 1, a draw plate 2 is fixedly sleeved on the top of the clamp plate 1, stop holes 3 are formed on the top of the draw plate 2, the number of the stop holes 3 is six, deep tubes 4 are fixedly mounted on the bottom of the clamp plate 1, the number of the deep tubes 4 is six, a mechanical transmission plate 5 is arranged above the clamp plate 1, sleeves 6 are fixedly mounted on the bottom of the mechanical transmission plate 5, the number of the sleeves 6 is six, a suction tube 7 is fixedly sleeved on the outer surface of the sleeve 6, a sealing device 8 is movably sleeved inside the stop holes 3, the inner wall of the stop holes 3 is stepped and is matched with the sealing device 8, the sealing device 8 includes a sealing sleeve 81, the top of the sealing sleeve 81 is made of soft rubber, a support ring 82 is fixedly sleeved on the bottom of the outer surface of the sealing sleeve 81, the support ring 82 is matched with a stepped structure arranged on the, fixed mounting has holding ring 83 in the position that support ring 82 bottom is close to the outside, one-way via hole 84 has been seted up to the bottom of seal cover 81, the top aperture of one-way via hole 84 is greater than the bottom aperture, fixed mounting has spacing pipe 85 in the position that the bottom of seal cover 81 inner chamber is located one-way via hole 84 top, deformation hole 86 has been seted up at the top of seal cover 81, the aperture of deformation hole 86 is greater than the pipe diameter of straw 7 and the aperture of deformation hole 86 is less than the diameter of sleeve 6, the internal diameter of spacing pipe 85 is greater than the diameter of straw 7 and the same with the diameter of sleeve 6, the axis of straw 7 and sealing device 8's axis are located same central line, the axis of straw 7 and the axis of deep tube 4 are.
Wherein, the aperture through the deformation hole 86 is larger than the pipe diameter of the straw 7 and the aperture of the deformation hole 86 is smaller than the diameter of the sleeve 6, so that the straw 7 firstly passes through the deformation hole 86 and the limiting pipe 85 and contacts with the one-way through hole 84 at the bottom of the sealing sleeve 81 in the downward movement process of the sleeve 6, the top of the sealing sleeve 81 is made of soft rubber, so that the straw 7 can extrude the one-way through hole 84 and extend into the deep pipe 4, as the straw 7 continues to move downwards, the sleeved part of the tail end of the straw 7 and the sleeve 6 can contact with the deformation hole 86 at the top of the sealing sleeve 81 and make the deformation hole 86 and the sleeve 6 joint and move downwards, because the inner diameter of the limiting pipe 85 is larger than the diameter of the straw 7 and is the same as the diameter of the sleeve 6, the deformation hole 86 can contact with the top of the limiting pipe 85 when the deformation hole 86 and the sleeve 6 move downwards, at the deformation hole 86 can not continue to deform and simultaneously make the sealing device 8 finish the The body and the suction pipe 7 move downwards synchronously, and along with the extension of the suction pipe 7 and the sealing device 8 into the deep pipe 4, the volume in the deep pipe 4 is compressed, so that the deep pipe 4 passes throughLifting upStraw 7 discharges and forms one-way air current in straw 7 for outside impurity can't enter into the inside of deep tube 4 through straw 7, has avoided outside volatile gas or other impurity to pollute medicament or the sample in the deep tube 4, has improved the purity of nucleic acid extraction.
Wherein, through accomplishing the extraction operation when the straw 7 and making the straw 7 draw from the deep tube 4 through the motion of sleeve 6 and separating, sealing device 8 moves through the frictional force synchronization of straw 7 this moment wholly, sealing device 8 reaches the stroke limit when support ring 82 takes place to contact with sleeve 6 this moment, straw 7 draws from sealing device 8's inside this moment, when straw 7 breaks away from with one-way via hole 84, one-way via hole 84 resets again and through the top aperture of one-way via hole 84 be greater than the bottom aperture and prevent the inside gas of deep tube 4 from leaking, thereby avoided the gas that volatilizees in the deep tube 4 to enter the inside reagent or the sample that leads to other deep tubes 4 of operation box and receive the pollution, reduced the probability that is polluted by debris in the deep tube 4.
Wherein, it is corresponding with the position of deep tube 4 to go up the position that fixed mounting has holding ring 83 and holding ring 83 through the support ring 82 bottom near the outside for pair through holding ring 83 and deep tube 4 when installing taking out board 2 and make that sealing device 8 can be better go on to it with deep tube 4, avoided appearing taking out the mounted position of board 2 and appearing the deviation and lead to the unable normal condition that enters into deep tube 4 of sealing device 8, improved the device job stabilization nature.
The using method of the invention is as follows:
when the suction pipe is used, the suction pipe 7 firstly passes through the deformation hole 86 and the limiting pipe 85 and contacts with the one-way through hole 84 at the bottom of the sealing sleeve 81 in the downward movement process of the sleeve 6, so that the suction pipe 7 can extrude out the one-way through hole 84 and extend into the deep pipe 4, as the suction pipe 7 continues to move downward, the sleeved part of the tail end of the suction pipe 7 and the sleeve 6 can contact with the deformation hole 86 at the top of the sealing sleeve 81 and make the deformation hole 86 fit with the sleeve 6 and move downward, when the deformation hole 86 and the sleeve 6 move downward, the deformation hole 86 can contact with the top of the limiting pipe 85, at the moment, the deformation hole 86 cannot continuously deform, and simultaneously the sealing device 8 and the suction pipe 7 synchronously move downward through the friction force between the deformation hole 86 and the suction pipe 7, as the suction pipe 7 and the sealing device 8 extend into the deep pipe 4, the volume in the deep pipe 4 is compressed, so that the suction pipe 4 is lifted up and discharged through the suction pipe 7 and forms one-way air flow, so that foreign substances from the outside cannot enter the inside of the deep tube 4 through the suction pipe 7.
After use, the suction pipe 7 is drawn out from the deep tube 4 by the movement of the sleeve 6, the sealing device 8 is moved synchronously by the friction force of the suction pipe 7, the sealing device 8 reaches the stroke limit when the support ring 82 is contacted with the sleeve 6, the suction pipe 7 is drawn out from the inside of the sealing device 8, when the suction pipe 7 is separated from the one-way through hole 84, the one-way through hole 84 is reset again, and the top aperture through the one-way through hole 84 is larger than the bottom aperture so as to prevent the gas inside the deep tube 4 from leaking.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. An automatic nucleic acid extraction method comprises a splint (1), and is characterized in that: the drawing plate (2) is fixedly sleeved at the top of the clamping plate (1), the top of the drawing plate (2) is provided with stroke stopping holes (3) and the number of the stroke stopping holes (3) is six, the bottom of the clamping plate (1) is fixedly provided with deep tubes (4) and the number of the deep tubes (4) is six, a mechanical transmission plate (5) is arranged above the clamping plate (1), the bottom of the mechanical transmission plate (5) is fixedly provided with sleeves (6) and the number of the sleeves (6) is six, the outer surface of each sleeve (6) is fixedly sleeved with a suction pipe (7), and the inner part of each stroke stopping hole (3) is movably sleeved with a sealing device (8);
the automatic nucleic acid extraction method comprises the following steps:
s1: the suction pipe (7) firstly passes through the deformation hole (86) and the limiting pipe (85) and is in contact with the one-way through hole (84) in the bottom of the sealing sleeve (81) in the downward movement process of the sleeve (6), so that the suction pipe (7) can extrude out the one-way through hole (84) and extend into the deep pipe (4), along with the continuous downward movement of the suction pipe (7), the part, sleeved with the sleeve (6), of the tail end of the suction pipe (7) can be in contact with the deformation hole (86) in the top of the sealing sleeve (81), the deformation hole (86) is attached to the sleeve (6) and moves downwards, and when the deformation hole (86) and the sleeve (6) move downwards, the deformation hole (86) can be in contact with the top of the limiting pipe (85).
S2: deformation hole (86) can not continue to take place deformation and simultaneously make sealing device (8) whole and straw (7) synchronous downstream through the frictional force between with straw (7), along with straw (7) and sealing device (8) inside stretching into to deep tube (4), compress in the volume in deep tube (4), make mentioning in the deep tube (4) discharge and form unidirectional air current in straw (7) through straw (7), make outside impurity can't enter into the inside of deep tube (4) through straw (7).
S3: the movement of the sleeve 6 enables the suction pipe (7) to be drawn out of the deep pipe (4), the sealing device (8) moves synchronously through the friction force of the suction pipe (7), when the support ring (82) is in contact with the sleeve (6), the sealing device (8) reaches the stroke limit, the suction pipe (7) is drawn out of the sealing device (8), when the suction pipe (7) is separated from the one-way through hole (84), the one-way through hole (84) resets again, and the top aperture of the one-way through hole (84) is larger than the bottom aperture, so that gas inside the deep pipe (4) is prevented from leaking.
2. The method for automatically extracting nucleic acid according to claim 1, wherein: the inner wall of the stop hole (3) is arranged in a step shape and is matched with the sealing device (8).
3. The method for automatically extracting nucleic acid according to claim 2, wherein: sealing device (8) are including seal cover (81), the fixed cover in bottom of seal cover (81) surface has connect support ring (82), support ring (82) and the echelonment structure that ends journey hole (3) inner wall set up mutually support, the position that fixed mounting has holding ring (83) and holding ring (83) on the position that support ring (82) bottom is close to the outside is corresponding with the position of deep tube (4), one-way clearing hole (84) have been seted up to the bottom of seal cover (81), fixed mounting has spacing pipe (85) on the position that the bottom of seal cover (81) inner chamber is located one-way clearing hole (84) top, deformation hole (86) have been seted up at the top of seal cover (81).
4. The method for automatically extracting nucleic acid according to claim 1, wherein: the axis of the suction pipe (7) and the axis of the sealing device (8) are located on the same central line, and the axis of the suction pipe (7) and the axis of the deep pipe (4) are located on the same central line.
5. The method for automatically extracting nucleic acid according to claim 3, wherein: the top of the sealing sleeve (81) is made of soft rubber.
6. The method for automatically extracting nucleic acid according to claim 3, wherein: the aperture of the deformation hole (86) is larger than the pipe diameter of the suction pipe (7), the aperture of the deformation hole (86) is smaller than the diameter of the sleeve (6), and the inner diameter of the limiting pipe (85) is larger than the diameter of the suction pipe (7) and is the same as the diameter of the sleeve (6).
7. The method for automatically extracting nucleic acid according to claim 3, wherein: the one-way passing hole (84) has a larger top aperture than a bottom aperture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011481353.XA CN112592813A (en) | 2020-12-16 | 2020-12-16 | Automatic nucleic acid extraction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011481353.XA CN112592813A (en) | 2020-12-16 | 2020-12-16 | Automatic nucleic acid extraction method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112592813A true CN112592813A (en) | 2021-04-02 |
Family
ID=75196214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011481353.XA Withdrawn CN112592813A (en) | 2020-12-16 | 2020-12-16 | Automatic nucleic acid extraction method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112592813A (en) |
-
2020
- 2020-12-16 CN CN202011481353.XA patent/CN112592813A/en not_active Withdrawn
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| PB01 | Publication | ||
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| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210402 |
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| WW01 | Invention patent application withdrawn after publication |