CN112586603A - Preparation method of high-nutrition digestion-promoting pet feed - Google Patents

Preparation method of high-nutrition digestion-promoting pet feed Download PDF

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CN112586603A
CN112586603A CN202011478777.0A CN202011478777A CN112586603A CN 112586603 A CN112586603 A CN 112586603A CN 202011478777 A CN202011478777 A CN 202011478777A CN 112586603 A CN112586603 A CN 112586603A
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谭孝花
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Chengdu Jianxiujun Technology Co ltd
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
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    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/22—Compounds of alkali metals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
    • A23K50/42—Dry feed

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Abstract

The invention belongs to the technical field of pet feed, and particularly relates to a preparation method of high-nutrition digestion-promoting pet feed. The high-nutrition digestion-promoting pet feed prepared by the invention can well provide a good carrier due to the composite bone powder contained in the feed, can attract a pet to eat, can be grafted with the surface of dietary fiber, can be better digested by the bone powder and the dietary fiber, and can promote absorption, and can well perform chewing digestion to provide nutrient substances due to the bone powder through high-temperature cooking, the action of the additive A and ultrasonic oscillation treatment, and finally can further perform nutrition supplement by the additive, and polypeptide supplement, so that the self regulation of the pet is promoted, and the immunity is improved.

Description

Preparation method of high-nutrition digestion-promoting pet feed
Technical Field
The invention belongs to the technical field of pet feed, and particularly relates to a preparation method of high-nutrition digestion-promoting pet feed.
Background
The pet food is a food specially provided for pets and small animals, and is a high-grade animal food between human food and traditional livestock and poultry feed. At present, the number of pets in China is increased every year, the quality requirement of pet food is stricter, and the pet owner can select the pet food more carefully. As a new industry, the demand of pet food is gradually rising, and the key point of the research of the pet food is the nutrition collocation and whether the digestibility of the gastrointestinal tract of the pet can be improved. Along with the gradual increase and perfection of the pet food market, the pet food market is more and more valued by people, and many scientific researchers are developing new pet food, so that the development of the pet food has considerable prospects.
At present, domestic pets mainly comprise dogs and cats, and the digestion processes of the dogs and the cats are obviously different, wherein the dogs are omnivores and the cats are carnivores. However, they also have some similar properties, such as lack of salivary amylase and short gastrointestinal tract which cannot synthesize vitamin D.
At present, pet feed on the market is mainly divided into main feed, auxiliary feed, health care feed and the like. Wherein the main feed is required to have good palatability, easy preservation and easy carrying. The auxiliary feed is feed with single nutrient and bright color. Its main function is to regulate appetite, some are for animals to grind teeth or lick plates. The health feed is a feed which is added with specific nutrients, can meet special nutritional requirements of animals and can promote body functions, but the current pet feed has low nutritional value and is easy to cause the problems of indigestion and the like.
Disclosure of Invention
The technical problems to be solved by the invention are as follows: aiming at the problems that the pet feed is low in nutritive value and is easy to cause feed indigestion, the preparation method of the high-nutrition digestion-promoting pet feed is provided.
In order to solve the technical problems, the invention adopts the following technical scheme:
a preparation method of a high-nutrition digestion-promoting pet feed comprises the following steps:
(1) mixing the mixed bone meal, ammonia water and an additive A according to a mass ratio of 9:18: 1-3, cooking, filtering, washing, drying, collecting a dried substance, and performing ultrasonic oscillation on the dried substance and a Tris-HCl buffer solution according to a solid-to-liquid ratio of g/mL1: 13-15 to obtain a mixture;
(2) putting the mixture and dopamine hydrochloride into a reactor according to a mass ratio of 20: 3-8, carrying out oxygen protection, stirring for reaction, discharging, filtering, washing, and drying to obtain a matrix;
(3) uniformly mixing dietary fiber and an ethanol solution according to a mass ratio of 1:3, performing steam explosion, filtering, collecting filter residues, and taking 180-190 parts by weight of an ethanol solvent, 80-90 parts by weight of filter residues, 60 parts by weight of a matrix, 20-30 parts by weight of an additive, 4-6 parts by weight of an emulsifier and 1-3 parts by weight of a cross-linking agent;
(4) firstly, uniformly stirring ethanol solvent, filter residue, additive and emulsifier, adjusting pH, adding cross-linking agent, heating, stirring, cooling, and freeze-drying to obtain the high-nutrition digestion-promoting pet feed.
The preparation method of the mixed bone powder in the step (1) comprises the steps of putting pig bones, ox bones and fish bones into a grinder according to the mass ratio of 5-9: 4-6: 5, grinding, sieving, collecting sieved particles, and obtaining the mixed bone powder.
The additive in the step (1) is formed by mixing sodium bicarbonate and sodium chloride according to a mass ratio of 5: 3-8.
The Tris-HCl buffer solution in the step (1) is a Tris-HCl buffer solution with the pH value of 8.5.
The preparation method of the additive comprises the following steps: crushing edible fly maggots, lemons and water according to a mass ratio of 7:1: 4-7, grinding, collecting a grinding mixture, mixing the grinding mixture and trypsin according to a mass ratio of 9:1 for enzymolysis, and freeze-drying to obtain the additive.
The emulsifier is one of sodium stearyl lactate and calcium stearyl lactate.
The cross-linking agent is formed by mixing glutaraldehyde and genipin according to the mass ratio of 1: 4-6.
Compared with other methods, the method has the beneficial technical effects that:
volatilization of compound bone meal-fibronectin coated essential oil
(1) The invention takes the mixed bone meal as a basic raw material, thereby determining that the invention is suitable for carnivorous animals, the porous bone meal is activated by soaking the bone meal in ammonia water for impurity removal and permeating the additive A and utilizing ultrasonic oscillation treatment, the surface of the bone meal is modified by utilizing dopamine hydrochloride, the surface activity is increased, the bone meal can be well combined with dietary fiber, the high-nutritive-value additive prepared by edible fly maggots and the like is mixed, and the bone meal can be well combined and fixed with the additive by utilizing a cross-linking agent to form the high-nutritive digestion-promoting pet feed;
(2) the high-nutrition digestion-promoting pet feed prepared by the invention can well provide a good carrier due to the composite bone powder contained in the feed, can attract a pet to eat, can be grafted with the surface of dietary fiber, can be better digested by the bone powder and the dietary fiber, and can promote absorption, and can well perform chewing digestion to provide nutrient substances due to the bone powder through high-temperature cooking, the action of the additive A and ultrasonic oscillation treatment, and finally can further perform nutrition supplement by the additive, and polypeptide supplement, so that the self regulation of the pet is promoted, and the immunity is improved.
Detailed Description
The preparation method of the mixed bone powder comprises the steps of putting pig bones, ox bones and fish bones into a grinder according to the mass ratio of 5-9: 4-6: 5, grinding for 30min at the speed of 1100r/min, sieving with a 200-mesh sieve, collecting sieved particles, and obtaining the mixed bone powder.
The additive A is formed by mixing sodium bicarbonate and sodium chloride according to a mass ratio of 5: 3-8.
The Tris-HCl buffer is a Tris-HCl buffer with the pH value of 8.5.
The preparation method of the additive comprises the following steps: crushing edible fly maggots, lemons and water according to a mass ratio of 7:1: 4-7, grinding for 1h at 900r/min, collecting a grinding mixture, mixing the grinding mixture and trypsin according to a mass ratio of 9:1, carrying out enzymolysis, and freeze-drying to obtain the additive.
The emulsifier is one of sodium stearyl lactate and calcium stearyl lactate.
The cross-linking agent is formed by mixing glutaraldehyde and genipin according to the mass ratio of 1: 4-6.
A preparation method of a high-nutrition digestion-promoting pet feed comprises the following steps:
(1) mixing the mixed bone meal, 1.2mol/L ammonia water and an additive A according to a mass ratio of 9:18: 1-3, cooking for 10 hours at 100 ℃, filtering, washing, drying, collecting a dried substance, and ultrasonically oscillating the dried substance and a Tris-HCl buffer solution according to a solid-to-liquid ratio of g/mL1: 13-15 for 20 minutes to obtain a mixture;
(2) putting the mixture and dopamine hydrochloride into a reactor according to a mass ratio of 20: 3-8, stirring for 16 hours under the protection of oxygen, discharging, filtering, washing and drying to obtain a matrix;
(3) uniformly mixing dietary fibers and 1.1mol/L ethanol solution according to a mass ratio of 1:3, maintaining the pressure for 1min at 3.1MPa, performing steam explosion, filtering, and collecting filter residues, wherein 180-190 parts by weight of ethanol solvent, 80-90 parts by weight of filter residues, 60 parts by weight of matrix, 20-30 parts by weight of additive, 4-6 parts by weight of emulsifier and 1-3 parts by weight of cross-linking agent are taken;
(4) firstly, uniformly stirring ethanol solvent, filter residue, additive and emulsifier, adjusting the pH value to 6-7, adding a cross-linking agent, heating to 70-90 ℃, stirring for 8 hours, cooling to room temperature, and freeze-drying to obtain the high-nutrition digestion-promoting pet feed.
Example 1
The preparation method of the mixed bone powder comprises the steps of putting pig bones, ox bones and fish bones into a grinder according to the mass ratio of 5-9: 4-6: 5, grinding for 30min at the speed of 1100r/min, sieving with a 200-mesh sieve, collecting sieved particles, and obtaining the mixed bone powder.
The additive A is formed by mixing sodium bicarbonate and sodium chloride according to the mass ratio of 5: 8.
The Tris-HCl buffer is a Tris-HCl buffer with the pH value of 8.5.
The preparation method of the additive comprises the following steps: pulverizing edible fly maggot, lemon and water at a mass ratio of 7:1:7, grinding for 1h at 900r/min, collecting the grinding mixture, mixing the grinding mixture with trypsin at a mass ratio of 9:1, performing enzymolysis, and freeze drying to obtain the additive.
The emulsifier is one of sodium stearyl lactate and calcium stearyl lactate.
The cross-linking agent is prepared by mixing glutaraldehyde and genipin according to the mass ratio of 1: 6.
A preparation method of a high-nutrition digestion-promoting pet feed comprises the following steps:
(1) mixing the mixed bone meal, 1.2mol/L ammonia water and an additive A according to a mass ratio of 9:18:3, cooking for 10 hours at 100 ℃, filtering, washing, drying, collecting a dried substance, and ultrasonically oscillating the dried substance and a Tris-HCl buffer solution according to a solid-to-liquid ratio of g/mL1:15 for 20 minutes to obtain a mixture;
(2) putting the mixture and dopamine hydrochloride into a reactor according to the mass ratio of 20: 8, stirring for 16 hours under the protection of oxygen, discharging, filtering, washing and drying to obtain a matrix;
(3) uniformly mixing dietary fibers and 1.1mol/L ethanol solution according to a mass ratio of 1:3, maintaining the pressure at 3.1MPa for 1min, performing steam explosion, filtering, collecting filter residues, and taking 190 parts of ethanol solvent, 90 parts of filter residues, 60 parts of matrix, 30 parts of additive, 6 parts of emulsifier and 3 parts of cross-linking agent according to parts by weight;
(4) firstly, uniformly stirring ethanol solvent, filter residue, additive and emulsifier, adjusting pH to 7, adding cross-linking agent, heating to 90 ℃, stirring for 8h, cooling to room temperature, and freeze-drying to obtain the high-nutrition digestion-promoting pet feed.
Example 2
The preparation method of the mixed bone powder comprises the steps of putting pig bones, ox bones and fish bones into a pulverizer according to the mass ratio of 7:5:5, pulverizing for 30min at the speed of 1100r/min, sieving with a 200-mesh sieve, collecting sieved particles, and obtaining the mixed bone powder.
The additive A is formed by mixing sodium bicarbonate and sodium chloride according to the mass ratio of 5: 6.
The Tris-HCl buffer is a Tris-HCl buffer with the pH value of 8.5.
The preparation method of the additive comprises the following steps: pulverizing edible fly maggot, lemon and water at a mass ratio of 7:1:5, grinding for 1h at 900r/min, collecting the grinding mixture, mixing the grinding mixture with trypsin at a mass ratio of 9:1, performing enzymolysis, and freeze drying to obtain the additive.
The emulsifier is one of sodium stearyl lactate and calcium stearyl lactate.
The cross-linking agent is prepared by mixing glutaraldehyde and genipin according to the mass ratio of 1: 5.
A preparation method of a high-nutrition digestion-promoting pet feed comprises the following steps:
(1) mixing the mixed bone meal, 1.2mol/L ammonia water and an additive A according to a mass ratio of 9:18:2, cooking for 10 hours at 100 ℃, filtering, washing, drying, collecting a dried substance, and ultrasonically oscillating the dried substance and a Tris-HCl buffer solution according to a solid-to-liquid ratio of g/mL1:14 for 20 minutes to obtain a mixture;
(2) putting the mixture and dopamine hydrochloride into a reactor according to the mass ratio of 20:6, stirring for 16 hours under the protection of oxygen, discharging, filtering, washing and drying to obtain a matrix;
(3) uniformly mixing dietary fibers and 1.1mol/L ethanol solution according to a mass ratio of 1:3, maintaining the pressure at 3.1MPa for 1min, performing steam explosion, filtering, collecting filter residues, and taking 185 parts of ethanol solvent, 85 parts of filter residues, 60 parts of matrix, 25 parts of additive, 5 parts of emulsifier and 2 parts of cross-linking agent according to parts by weight;
(4) firstly, uniformly stirring ethanol solvent, filter residue, additive and emulsifier, adjusting pH to 7, adding cross-linking agent, heating to 80 ℃, stirring for 8h, cooling to room temperature, and freeze-drying to obtain the high-nutrition digestion-promoting pet feed.
Example 3
The preparation method of the mixed bone powder comprises the steps of putting pig bones, ox bones and fish bones into a pulverizer according to the mass ratio of 5:4:5, pulverizing for 30min at the speed of 1100r/min, sieving with a 200-mesh sieve, collecting sieved particles, and obtaining the mixed bone powder.
The additive A is formed by mixing sodium bicarbonate and sodium chloride according to the mass ratio of 5: 3.
The Tris-HCl buffer is a Tris-HCl buffer with the pH value of 8.5.
The preparation method of the additive comprises the following steps: pulverizing edible fly maggot, lemon and water at a mass ratio of 7:1:4, grinding for 1h at 900r/min, collecting the grinding mixture, mixing the grinding mixture with trypsin at a mass ratio of 9:1, performing enzymolysis, and freeze drying to obtain the additive.
The emulsifier is one of sodium stearyl lactate and calcium stearyl lactate.
The cross-linking agent is formed by mixing glutaraldehyde and genipin according to the mass ratio of 1: 4.
A preparation method of a high-nutrition digestion-promoting pet feed comprises the following steps:
(1) mixing the mixed bone meal, 1.2mol/L ammonia water and an additive A according to the mass ratio of 9:18:1, cooking for 10 hours at 100 ℃, filtering, washing, drying, collecting a dried substance, and ultrasonically oscillating the dried substance and a Tris-HCl buffer solution according to the solid-to-liquid ratio of g/mL1:13 for 20 minutes to obtain a mixture;
(2) putting the mixture and dopamine hydrochloride into a reactor according to the mass ratio of 20:3, carrying out oxygen protection, stirring for reaction for 16 hours, discharging, filtering, washing and drying to obtain a matrix;
(3) uniformly mixing dietary fibers and 1.1mol/L ethanol solution according to a mass ratio of 1:3, maintaining the pressure at 3.1MPa for 1min, performing steam explosion, filtering, collecting filter residues, and taking 180-190 parts by weight of ethanol solvent, 80 parts by weight of filter residues, 60 parts by weight of matrix, 20 parts by weight of additive, 4 parts by weight of emulsifier and 1 part by weight of cross-linking agent;
(4) firstly, uniformly stirring ethanol solvent, filter residue, additive and emulsifier, adjusting pH to 6, adding cross-linking agent, heating to 70 ℃, stirring for 8h, cooling to room temperature, and freeze-drying to obtain the high-nutrition digestion-promoting pet feed.
Comparative example 1
The preparation method of the additive comprises the following steps: pulverizing edible fly maggot, lemon and water at a mass ratio of 7:1:5, grinding for 1h at 900r/min, collecting the grinding mixture, mixing the grinding mixture with trypsin at a mass ratio of 9:1, performing enzymolysis, and freeze drying to obtain the additive.
The emulsifier is one of sodium stearyl lactate and calcium stearyl lactate.
The cross-linking agent is prepared by mixing glutaraldehyde and genipin according to the mass ratio of 1: 5.
A preparation method of a high-nutrition digestion-promoting pet feed comprises the following steps:
(1) uniformly mixing dietary fibers and 1.1mol/L ethanol solution according to a mass ratio of 1:3, maintaining the pressure at 3.1MPa for 1min, performing steam explosion, filtering, collecting filter residues, and taking 185 parts of ethanol solvent, 80-90 parts of filter residues, 25 parts of additive, 5 parts of emulsifier and 2 parts of cross-linking agent according to parts by weight;
(2) firstly, uniformly stirring ethanol solvent, filter residue, additive and emulsifier, adjusting pH to 7, adding cross-linking agent, heating to 80 ℃, stirring for 8h, cooling to room temperature, and freeze-drying to obtain the high-nutrition digestion-promoting pet feed.
Comparative example 2
The preparation method of the mixed bone powder comprises the steps of putting pig bones, ox bones and fish bones into a pulverizer according to the mass ratio of 7:5:5, pulverizing for 30min at the speed of 1100r/min, sieving with a 200-mesh sieve, collecting sieved particles, and obtaining the mixed bone powder.
The additive A is formed by mixing sodium bicarbonate and sodium chloride according to the mass ratio of 5: 6.
The Tris-HCl buffer is a Tris-HCl buffer with the pH value of 8.5.
The preparation method of the additive comprises the following steps: pulverizing edible fly maggot, lemon and water at a mass ratio of 7:1:5, grinding for 1h at 900r/min, collecting the grinding mixture, mixing the grinding mixture with trypsin at a mass ratio of 9:1, performing enzymolysis, and freeze drying to obtain the additive.
The emulsifier is one of sodium stearyl lactate and calcium stearyl lactate.
The cross-linking agent is prepared by mixing glutaraldehyde and genipin according to the mass ratio of 1: 5.
A preparation method of a high-nutrition digestion-promoting pet feed comprises the following steps:
(1) mixing the mixed bone meal, 1.2mol/L ammonia water and an additive A according to a mass ratio of 9:18:2, cooking for 10 hours at 100 ℃, filtering, washing, drying, collecting a dried substance, and ultrasonically oscillating the dried substance and a Tris-HCl buffer solution according to a solid-to-liquid ratio of g/mL1:14 for 20 minutes to obtain a mixture;
(2) putting the mixture and dopamine hydrochloride into a reactor according to the mass ratio of 20:6, stirring for 16 hours under the protection of oxygen, discharging, filtering, washing and drying to obtain a matrix;
(3) 185 parts of ethanol solvent, 60 parts of matrix, 25 parts of additive, 5 parts of emulsifier and 2 parts of cross-linking agent;
(4) firstly, uniformly stirring ethanol solvent, filter residue, additive and emulsifier, adjusting pH to 7, adding cross-linking agent, heating to 80 ℃, stirring for 8h, cooling to room temperature, and freeze-drying to obtain the high-nutrition digestion-promoting pet feed.
The high-nutrition digestion promoting pet feed obtained in the embodiment and the high-nutrition digestion promoting pet feed of the comparative example are detected, and the specific detection is as follows: the pet is selected to be a dog;
feeding tests were conducted on dogs using the high nutrition digestion promoting pet food of the present invention and the high nutrition digestion promoting pet food of the control example. 50 freshly weaned pups with good health status were individually selected as test subjects and randomly assigned to the example group and the control group. Each group was fed continuously with a corresponding high nutrient digestion promoting pet food. Feeding 2 meals at regular time every day, feeding the same amount of dog food every meal, continuously feeding for 30 days, ensuring that each test dog can not contact other food two groups respectively, performing intraperitoneal injection on the test pet dog at 2 months, 4 months and 6 months, performing intraperitoneal injection on 1 mL/intraperitoneal injection of diluted inactivated BCG vaccine after 24 hours, extracting peritoneal fluid after 0.5 hour, quickly coating the peritoneal fluid on a clean sterilized slide, incubating in a wet box for 1.0 hour, slightly washing with normal saline, removing cells which are not attached to the wall, drying, fixing with methanol, and counting 300 macrophages under an oil microscope after acid-fast staining. Phagocytic activity is expressed by the following indices: percent phagocytosis: (number of peritoneal macrophages phagocytosing tubercle bacillus/number of peritoneal macrophages of basal number) × 100%. Phagocytosis index: i.e. the average number of tubercle bacilli phagocytosed per macrophage (number of phagocytosed tubercle bacilli/number of counted abdominal macrophages). At month 6 percent phagocytosis and phagocytosis index were recorded and the experimental data are shown in table 1.
Table 1:
Figure 409115DEST_PATH_IMAGE002
in conclusion, the high-nutrition digestion-promoting pet feed disclosed by the invention is good in feeding effect, higher in resistance after being eaten, high in digestibility and worthy of being popularized vigorously.

Claims (7)

1. A preparation method of a high-nutrition digestion-promoting pet feed is characterized by comprising the following steps:
(1) mixing the mixed bone meal, ammonia water and an additive A according to a mass ratio of 9:18: 1-3, cooking, filtering, washing, drying, collecting a dried substance, and performing ultrasonic oscillation on the dried substance and a Tris-HCl buffer solution according to a solid-to-liquid ratio of g/mL1: 13-15 to obtain a mixture;
(2) putting the mixture and dopamine hydrochloride into a reactor according to a mass ratio of 20: 3-8, carrying out oxygen protection, stirring for reaction, discharging, filtering, washing, and drying to obtain a matrix;
(3) uniformly mixing dietary fiber and an ethanol solution according to a mass ratio of 1:3, performing steam explosion, filtering, collecting filter residues, and taking 180-190 parts by weight of an ethanol solvent, 80-90 parts by weight of filter residues, 60 parts by weight of a matrix, 20-30 parts by weight of an additive, 4-6 parts by weight of an emulsifier and 1-3 parts by weight of a cross-linking agent;
(4) firstly, uniformly stirring ethanol solvent, filter residue, additive and emulsifier, adjusting pH, adding matrix substance and cross-linking agent, heating, stirring, cooling, and freeze-drying to obtain the high-nutrition digestion-promoting pet feed.
2. The preparation method of the high-nutrition digestion-promoting pet feed as claimed in claim 1, wherein the mixed bone powder in the step (1) is prepared by putting pig bones, cattle bones and fish bones into a pulverizer according to a mass ratio of 5-9: 4-6: 5, pulverizing, sieving, and collecting sieved particles to obtain the mixed bone powder.
3. The preparation method of the high-nutrition digestion-promoting pet feed according to claim 1, wherein the additive A in the step (1) is formed by mixing sodium bicarbonate and sodium chloride according to a mass ratio of 5: 3-8.
4. The method for preparing a highly nutritious digestion promoting pet food as claimed in claim 1, wherein the Tris-HCl buffer solution in step (1) is Tris-HCl buffer solution with pH 8.5.
5. The method for preparing a highly nutritious digestion promoting pet food as claimed in claim 1, wherein the method for preparing the additive is: crushing edible fly maggots, lemons and water according to a mass ratio of 7:1: 4-7, grinding, collecting a grinding mixture, mixing the grinding mixture and trypsin according to a mass ratio of 9:1 for enzymolysis, and freeze-drying to obtain the additive.
6. The method for preparing a pet food with high nutrition and digestion promotion functions as claimed in claim 1, wherein the emulsifier is one of sodium stearoyl lactylate and calcium stearoyl lactylate.
7. The preparation method of the high-nutrition digestion-promoting pet feed according to claim 1, wherein the cross-linking agent is formed by mixing glutaraldehyde and genipin according to a mass ratio of 1: 4-6.
CN202011478777.0A 2020-12-16 2020-12-16 Preparation method of high-nutrition digestion-promoting pet feed Pending CN112586603A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115039836A (en) * 2022-06-27 2022-09-13 泉州师范学院 Pet dog teeth grinding rod rich in fishbone paste and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115039836A (en) * 2022-06-27 2022-09-13 泉州师范学院 Pet dog teeth grinding rod rich in fishbone paste and preparation method thereof

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